Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| effective ileal loop dose of kanagawa-positive vibrio parahaemolyticus. | groups of 16 rabbits per strain were injected with broth culture dilutions of three kanagawa-positive vibrio parahaemolyticus strains. the effective dose required to produce ileal loop dilatation in 50% of rabbits for pure cultures of strains 10136-76 and 553-72 from patients stools and ny 477 from incriminated food was 1.1 x 10(6), 2.6 x 10(5), and 7.7 x 10(6) organisms, respectively. when each of these cultures was admixed with greater than or equal to 10(9) vibrio alginolyticus cells, the 50% ... | 1980 | 7458308 |
| in vitro antibiotic sensitivity testing of vibrio alginolyticus. | v. alginolyticus from environmental sources of different geographical areas and some human pathogenic strains were investigated for their sensitivity to 29 different antimicrobial agents, using the agar diffusion method. all strains were sensitive to gentamicin, neomycin, sulfaisodimidin, sulfamethoxazole-trimethoprim, rifamycin, nalidixan and linco-spectin and all except one to trimethoprim, tetracycline, chloramphenicol, nitrofurantoin and tobramycin. between 80 and 94% of tested strains were ... | 1980 | 7223432 |
| a comparative study of the sugar composition of o-antigenic lipopolysaccharides isolated from vibrio alginolyticus and vibrio parahaemolyticus. | a comparative study of the sugar composition of o-antigenic lipopolysaccharides (lps) isolated from vibrio alginolyticus and those from v. parahaemolyticus was carried out. 3-deoxy-d-mannooctulosonic acid, 2-keto-3-deoxy octonate (kdo), a regular sugar constituent of gram-negative bacterial lps, was totally absent from lps of all v. alginolyticus strains examined as it was from those of v. parahaemolyticus. furthermore, a kdo-like thiobarbituric acid test-positive substance, identical with that ... | 1981 | 7231217 |
| a comprehensive study of environmental and human pathogenic vibrio alginolyticus strains. | an investigation comprising 49 environmental (vae), 7 human isolates (vah) and 3 type culture (vat) strains of vibrio alginolyticus showed a very high similarity among the strains. by primary isolation a swarming activity on blood agar and a yellow, hemispheric relatively large colony type on tcbs were very conspicuous. besides the characteristics of vibrionaceae the most typical and differential diagnostic important features were: sucrose fermentation, lysine decarboxylase activity, acetoine an ... | 1981 | 7200302 |
| incidence of vibrio alginolyticus and bacteria of sanitary significance in the bering sea. | the incidence of bacteria of sanitary significance and isolation of vibrio alginolyticus during a subtidal clam assessment study in the bering sea are described. vibrio parahaemolyticus was not detected in clams, sediment, or seawater. | 1981 | 7259158 |
| occurrence of vibrio parahaemolyticus and vibrio alginolyticus in marine and estuarine bathing areas in danish coast. | from seventeen sites in three marine and two estuarine bathing areas at the danish coast water samples were collected during the period from may to november 1979. total bacterial counts were performed on blood agar. the prevalence of vibrio parahaemolyticus and vibrio alginolyticus in 100 ml water samples using the filter membrane technique and tcbs agar plates was investigated with the possible correlation to water temperature and salinity. only five strains of v. parahaemolyticus were isolated ... | 1981 | 7282164 |
| potassium ion is required for the generation of ph-dependent membrane potential and delta ph by the marine bacterium vibrio alginolyticus. | the electrochemical potential gradient of protons in the marine bacterium vibrio alginolyticus was measured as a function of external ph. in k+-containing cells, the membrane potential (delta psi) and delta ph vary with external ph as reported in other bacteria. on the other hand, k+-depleted cells show little ph dependence in the magnitude of delta psi from ph 6.0 to 8.5. the cytoplasmic ph in these cells varies depending on external ph, resulting in the generation of a small delta ph at acidic ... | 1981 | 7284321 |
| a respiration-dependent primary sodium extrusion system functioning at alkaline ph in the marine bacterium vibrio alginolyticus. | 1981 | 7306152 | |
| isolation and characterization of vibrio alginolyticus and vibrio parahaemolyticus from the norwegian coastal environment. | 1981 | 7344524 | |
| [finding of vibrio parahaemolyticus and vibrio alginolyticus in marine sediment at the mouth of the tiber]. | 1981 | 7345400 | |
| vibrio alginolyticus peritonitis associated with ambulatory peritoneal dialysis. | 1981 | 6788288 | |
| partial purification and properties of respiratory chain-linked l-glycerol 3-phosphate dehydrogenase from a marine bacterium, vibrio alginolyticus. | respiratory chain-linked l-glycerol 3-phosphate (g3p) dehydrogenase [ec 1.1.99.5] of marine bacterium, vibrio alginolyticus, was extracted from the membrane fraction by treatment with tween 20, and fractionation on deae-sephacel and qae-sephadex in the presence of 0.05% liponox dch(alkyl polyoxyethylene ether) yielded a preparation having a specific activity of 22.1 units/mg protein when assayed by phenazine methosulfate (pms)-coupled reduction of thiazolyl blue tetrazolium (mtt). the purified e ... | 1981 | 6796567 |
| regulation of exoprotease production by temperature and oxygen in vibrio alginolyticus. | the production of an extracellular collagenase and alkaline protease by vibrio alginolyticus during stationary phase was inhibited by a temperature shift from 30 to 37 degrees c and by a lack of oxygen. the stability of the exoproteases was unaffected by incubation at 37 degrees c and aeration. the optimum growth temperature for the v. alginolyticus strain was 33.5 degrees c and there was no difference in the growth rate at 30 and 37 degrees c. aeration enhanced the rate of growth of exponential ... | 1981 | 6277266 |
| regulation of extracellular alkaline protease activity by histidine in a collagenolytic vibrio alginolyticus strain. | vibrio alginolyticus synthesized an inducible extracellular collagenase in a peptone medium during the stationary growth phase. these cultures also possessed extracellular alkaline serine protease activity. the alkaline protease activity did not require a specific inducer and it was produced in tryptone or minimal media. the collagenase was not produced in either the tryptone or minimal media. the alkaline protease activity was sensitive to catabolite repression by a number of carbon sources, in ... | 1981 | 6279766 |
| [comment on the review of the nursing encyclopedia]. | 1981 | 243904 | |
| [controlled research of the influence of neoston on tracking performance, heart rate, sinus arrhythmia and on subjective rating of healthy subjects]. | the influence of neoston--a relatively new analgesic, antipyretic, and anti-inflammatory agent--on performance in different tracking tests, on heart rate, on sinus arrhythmia and on subjective rating was objectivated. 15 healthy male and female subjects, aged from 19 to 27 years, took part in the sessions lasting about 2 1/2 hrs each. two times two pills neoston or a placebo were taken orally 2 and 5 hrs before the tests, making a total of 2 g. performance in tracking was poorer on the whole wit ... | 1982 | 242030 |
| histamine-producing bacteria in decomposing skipjack tuna (katsuwonus pelamis). | spoilage in skipjack tuna (katsuwonus pelamis) was studied under controlled conditions by incubating whole, fresh fish in seawater at 38 degrees c, the optimum temperature for histamine formation. bacterial isolates were obtained from the loin tissue of a decomposing tuna containing 134 mg of histamine per 100 g and a total anaerobic count of 3.5 x 10(5)/g after incubation for 24 h. over 92% of the 134 isolates obtained were facultatively or obligately anaerobic bacteria. eighteen isolates produ ... | 1982 | 6289747 |
| the mode of action of nanaomycins d and a on a gram-negative marine bacterium vibrio alginolyticus. | nanaomycin (nnm) d had a higher growth inhibitory activity than nnm-a against a gram-negative marine bacterium, vibrio alginolyticus. these quinone antibiotics were reduced by the respiratory chain-linked flavin dehydrogenase of the organism and the reduced forms of nnms were quickly autoxidized by molecular oxygen to produce superoxide radicals (o2-). nnm-d was more effective than nnm-a both in the induction of kcn-insensitive oxygen consumption with the intact cells and in the production of o2 ... | 1982 | 6292148 |
| market fish hygiene in kenya. | vibrio parahaemolyticus was isolated from 53 out of 584 samples (9.1%) of market fish. all strains were kanagawa negative and were distributed as follows: sea fish 5 out of 370 samples (1.4%), shellfish 48 out of 214 samles (22.4%). other fish spoilage microflora recovered were: alcaligenes faecalis, pseudomonas aeruginosa, proteus vulgaris, aeromonas spp. and vibrio alginolyticus. total aerobic counts and coliform counts per gram for the lake fish ranged from 2.6 x 10(2) to 6.6 x 10(7) and 10 t ... | 1982 | 6808058 |
| regulation of hut enzymes and intracellular protease activities in vibrio alginolyticus hut mutants. | the production of alkaline protease, collagenase and histidine utilization (hut) enzymes by vibrio alginolyticus wild-type, huth1 and hutu1 strains was investigated. alkaline protease synthesis was stimulated by histidine and urocanic acid in the wild-type and hutu1 strains. the huth1 mutant alkaline protease production was stimulated by urocanic acid and not by histidine. the hut enzymes in the wild-type strain were coordinately induced by histidine. urocanase and formimino-hydrolase were induc ... | 1982 | 6129284 |
| roles of na+ and k+ in alpha-aminoisobutyric acid transport by the marine bacterium vibrio alginolyticus. | effects of monovalent cations on alpha-aminoisobutyric acid (aib) transport were examined in the marine bacterium vibrio alginolyticus. in k+-containing cells, aib was actively accumulated only in the presence of na+, and the addition of k+ had essentially no effect. on the other hand, k+-depleted and na+-loaded cells required k+ as well as na+ for the accumulation of aib against its concentration gradient. the characterization of the roles of na+ and k+ in aib transport was performed by manipul ... | 1982 | 7054182 |
| characterization of the respiration-dependent na+ pump in the marine bacterium vibrio alginolyticus. | 1982 | 7107593 | |
| [vibrio alginolyticus as a cause of otitis media]. | 1982 | 7170691 | |
| microcalorimetric measurements of glucose metabolism by marine bacterium vibrio alginolyticus. | microcalorimetric measurements of heat production from glucose by vibrio alginolyticus were made to assess the viability of calorimetry as a technique for studying the metabolism of marine bacteria at organic nutrient concentrations found in marine waters. the results show that the metabolism of glucose by this bacterium can be measured by calorimetry at submicromolar concentrations. a linear correlation between glucose concentration and total heat production was observed over a concentration ra ... | 1982 | 16346131 |
| cellulitis caused by vibrio alginolyticus. | a case of cellulitis, resulting in a recurrence of a leg ulcer is presented due to the unique association of its bacterial flora, consisting exclusively of v. alginolyticus and of its clinically rapid expansion after seabathing. | 1983 | 6198855 |
| vibrios on the half shell: what the walrus and the carpenter didn't know. | at least nine vibrio species have been associated with disease in the united states. vibrio fluvialis, v. hollisae, v. mimicus, and v. parahaemolyticus cause diarrheal diseases, but may also be encountered in extraintestinal infections such as wound and ear infections, septicemia, and cholecystitis. vibrio alginolyticus, v. damsela, v. metschnikovii, and v. vulnificus primarily cause extraintestinal disease. toxigenic v. cholerae o1 is the cause of epidemic cholera, whereas nontoxigenic v. chole ... | 1983 | 6625388 |
| bacterial flora of marine penetrating injuries. | bacteriologic and clinical features of 28 cases of traumatic marine injuries are described. the most common bacterial isolates were normal skin bacteria, staphylococcus aureus and, in 11% of cases, vibrio alginolyticus. all injuries responded to local wound care, and only half received antibiotic therapy. | 1983 | 6673897 |
| medium for isolation and differentiation of vibrio parahaemolyticus and vibrio alginolyticus. | trypticase soy agar supplemented with sucrose, sodium chloride, bile salts, and triphenyltetrazolium chloride is an improved plating medium for the isolation of vibrio parahaemolyticus from samples of seawater, permitting better differentiation of this organism from vibrio alginolyticus and other bacteria. | 1983 | 6824320 |
| isolation of vibrio alginolyticus mutants defective in the respiration-coupled na+ pump. | when the respiration-coupled na+ pump functions, v. alginolyticus is able to grow in the presence of an extremely high concentration of proton conductor, carbonylcyanide m-chlorophenylhydrazone. the mutants which became sensitive to the proton conductor were isolated and examined in regard to the na+ pump activity. although the activity of a respiration-dependent h+ extrusion by the mutants is comparable to that by the wild type, the na+ pump activity of the mutants is significantly reduced. fur ... | 1983 | 6882417 |
| characterization of extracellular alkaline proteases and collagenase induction in vibrio alginolyticus. | the number and approximate molecular weights of extracellular alkaline proteases produced by vibrio alginolyticus were determined by gelatin-page. three major bands of protease activity with apparent molecular weights of approximately 28 000, 22 500 and 19 500 (proteases 1, 2 and 3, respectively) and two minor bands of protease activity with apparent molecular weights of approximately 15 500 and 14 500 (proteases 4 and 5, respectively) were obtained after gelatin-page. the activities of the five ... | 1983 | 6310026 |
| growth of a marine vibrio alginolyticus and moderately halophilic v. costicola becomes uncoupler resistant when the respiration-dependent na+ pump functions. | the growth of vibrio alginolyticus and v. costicola, which possess respiration-dependent na+ pumps, was highly resistant to the proton conductor carbonyl cyanide-m-chlorophenyl hydrazone (cccp), in alkaline growth media, even though the membrane was rendered permeable to h+. the ph dependence of cccp-resistant growth was similar to that of the na+ pump. in contrast, escherichia coli ml308-225 showed neither na+ pump activity nor cccp-resistant growth, even when grown in alkaline, na+-rich media. ... | 1983 | 6313611 |
| the absence of structural relationship between mitochondrial and mitochondrial and cytoplasmic leucyl-trna synthetases from tetrahymena pyriformis. | 1983 | 242273 | |
| kinetic investigation of unfolding and partial refolding of a crab satellite (da-dt)n. | crab (da-dt)n was isolated from the testes of cancer borealis by a procedure involving separation of dna and segregation of the satellite fraction by hg2+ binding/cs2so4 density gradient ultracentrifugation. the titration of crab (da-dt)n samples at 10 degrees indicated a sharp absorbance change at ph 11.98 in agreement with the phm value observed for synthetic poly(da-dt) under identical conditions. the reversal of the titration, however, resulted only in about 50% recovery of the original abso ... | 1984 | 240385 |
| in situ identification of bacterial species in marine microfouling films by using an immunofluorescence technique. | an immunofluorescence technique was developed for the in situ identification of specific bacteria in marine microfouling films. microorganisms adherent to glass plates after 30 days of immersion in a synthetic seawater system were cultured and classified by biochemical tests, flagellar arrangement, and the api 20e system. all isolates were gram-negative aerobic or facultative motile rods, predominantly pseudomonas spp. rabbit antisera to the five dominant organisms including achromobacter spp., ... | 1984 | 6393875 |
| the impact of terrestrial and estuarial factors on the density of environmental bacterial (vibrionaceae) and faecal coliforms in coastal water. | a study of the densities of faecal coliforms, vibrio anguillarum, vibrio alginolyticus and aeromonas hydrophila in danish bathing areas was carried out over the period may-october, 1981 with special reference to specific terrestrial and estuarial determinants. significant correlations were found between v. anguillarum and v. alginolyticus (r = 0.57) and a. hydrophila and faecal coliforms (r = 0.43). the densities of the vibrios were highly influenced by temperature and distance from pollution so ... | 1984 | 6395571 |
| halophilic vibrio species from seafish in senegal. | sucrose-positive and sucrose-negative halophilic vibrio species at counts of up to 10(7)/100 g were isolated from muscles tissue in 27 and 43%, respectively, of 128 seafish from coastal waters in senegal. vibrio parahaemolyticus, including 21% urease-positive strains, was the most common isolate, followed by vibrio alginolyticus, vibrio vulnificus, vibrio damsela, and vibrio fluvialis. | 1984 | 6476829 |
| k+/h+ antiporter functions as a regulator of cytoplasmic ph in a marine bacterium, vibrio alginolyticus. | the marine bacterium, vibrio alginolyticus, regulates the cytoplasmic ph at about 7.8 over the ph range 6.0-9.0. by the addition of diethanolamine (a membrane-permeable amine) at ph 9.0, the internal ph was alkalized and simultaneously the cellular k+ was released. following the k+ exit, the internal ph was acidified until 7.8, where the k+ exit leveled off. the k+ exit was mediated by a k+/h+ antiporter that is driven by the outwardly directed k+ gradient and ceases to function at the internal ... | 1984 | 6477914 |
| bacteriology of the teeth from a great white shark: potential medical implications for shark bite victims. | bacteria were cultured for the first time from the teeth of a great white shark (carcharodon carcharias). isolates included vibrio alginolyticus, vibrio fluvialis, vibrio parahaemolyticus, and other genera. all are common in the marine environment and some may be associated with wound infections in humans. shark bite lacerations may serve as a source of these potentially infectious bacteria, particularly vibrio spp., and should be treated immediately. antibiotic susceptibility patterns are shown ... | 1984 | 6511869 |
| solubilization and reconstitution of the na+-motive nadh oxidase activity from the marine bacterium vibrio alginolyticus. | the na+-motive nadh oxidase activity from vibrio alginolyticus was extracted with octylglucoside and reconstituted into liposomes by dilution. on the addition of nadh, the reconstituted proteoliposomes generated delta psi (inside positive) and delta ph (inside alkaline) in the presence of a proton conductor cccp, and accumulated na+ in the presence of valinomycin. these results indicate that the nadh oxidase activity, reconstituted in opposite orientation, leads to the generation of an electroch ... | 1984 | 6092131 |
| na+ is translocated at nadh:quinone oxidoreductase segment in the respiratory chain of vibrio alginolyticus. | the coupling site of the na+ pump to the respiratory chain of vibrio alginolyticus was examined using membrane fractions prepared from the wild type, na+ pump-deficient mutants, and spontaneous revertant. nadh oxidase of the wild type and revertant specifically required na+ for maximum activity, whereas na+ was not essential for the nadh oxidase of mutants. similar to the na+ pump in whole cells, the na+-dependent nadh oxidase in membranes had a ph optimum in the alkaline region. a respiratory i ... | 1984 | 6736026 |
| electrolyte effects on attachment of an estuarine bacterium. | the effect of electrolyte concentration on attachment of vibrio alginolyticus to hydroxyapatite was determined. bacterial affinity for attachment to the surface and surface capacity were derived from linearization of bacterial adsorption isotherms. at low concentrations (<0.1 m) the affinity of the bacteria for the surface increased with increasing ionic strength, in agreement with the d.l.v.o. theory of colloid interaction. at higher concentrations, bacterial affinity for the surface decreased ... | 1984 | 16346489 |
| sympathetically-induced development of tension in jaw muscles: the possible contraction of intrafusal muscle fibres. | in rabbits, cats and rats anaesthetized, curarized, with the skull fixed in a stereotaxic apparatus, the peripheral stump of the cervical sympathetic nerve (c.s.n.) was electrically stimulated at frequencies within the physiological range and the isometric tension was recorded at the lower jaw. in a group of experiments the afferent discharge from the jaw elevator muscle spindles was also recorded, in the mesencephalic nucleus of the fifth cranial nerve. unilateral stimulation of the c.s.n. indu ... | 1985 | 2934686 |
| plaque autoradiography assay for the detection and quantitation of thymidine kinase-deficient and thymidine kinase-altered mutants of herpes simplex virus in clinical isolates. | a plaque autoradiography assay to detect and quantitate thymidine kinase (tk) mutants of herpes simplex virus type 1 (hsv-1) and hsv-2 in clinical samples is described. this method utilizes the selective incorporation of [125i]iododeoxycytidine, a pyrimidine analog selectively phosphorylated by the hsv tk. only cells infected with tk-competent virus will efficiently incorporate iododeoxycytidine and are the only cells detected by autoradiography. furthermore, this assay discriminates between tk+ ... | 1985 | 3010836 |
| the na(+)-motive respiratory chain of marine bacteria. | the respiratory chain of the marine bacterium vibrio alginolyticus pumps out na+ at the nadh:quinone oxidoreductase segment. the respiratory na+ pump plays an important role in the bioenergetics of this bacterium by generating a sodium-motive force as a direct result of respiration in alkaline na(+)-rich environments. | 1985 | 2856376 |
| purification and regulation of glutamine synthetase in a collagenolytic vibrio alginolyticus strain. | glutamine synthetase (ec 6.3.1.2) has been purified from a collagenolytic vibrio alginolyticus strain. the apparent molecular weight of the glutamine synthetase subunit was approximately 62,000. this indicates a particle weight for the undissociated enzyme of 744,000, assuming the enzyme is the typical dodecamer. the glutamine synthetase enzyme had a sedimentation coefficient of 25.9 s and seems to be regulated by adenylylation and deadenylylation. the ph profiles assayed by the gamma-glutamyltr ... | 1985 | 2859007 |
| blood lipid variability in relation to relative weight and biochemical markers of tobacco and alcohol consumption. | carboxyhaemoglobin (cohb%) and gamma-glutamyl-transferase (ggt) are today frequently used as objective indicators of tobacco and alcohol consumption. the relationships between cohb%, ggt and relative body weight, cholesterol, triglyceride and apolipoprotein ai (apo-ai) were studied in middle-aged men attending a preventive medical programme in malmö, sweden. although statistically significant the influence of cohb% on cholesterol and triglyceride was found to be clinically insignificant. ggt and ... | 1985 | 2861602 |
| membrane-linked energy transductions. bioenergetic functions of sodium: h+ is not unique as a coupling ion. | the concept is developed according to which na+, like h+, can play the role of a coupling ion in energy-transducing biomembranes. this idea is based on observations that (i) na+ can be extruded from the cell by primary pumps (na-motive nadh-quinone reductase, decarboxylase or atpase), and (ii) the downhill na+ flux into the cell can be coupled with the performance of all the three types of membrane-linked work i.e. chemical (atp synthesis), osmotic (accumulation of solutes) and mechanical (motil ... | 1985 | 2863140 |
| [humoral factors of local and systemic immunity in the multiple peroral administration of a corpuscular pertussis vaccine]. | multiple oral immunization with pertussis corpuscular vaccine was shown to lead to the considerable stimulation of local and systemic humoral immunity. the data on the titers of specific and normal secretory antibodies, on the levels of iga in washings from the oral cavity, the small intestine and the lungs, on the titers of agglutinins and hemagglutinins in the blood serum, as well as on the morpho-functional transformation of the mucous membrane and the associated lymphoid tissue in the digest ... | 1985 | 2868593 |
| [humoral factors of local and systemic immunity in the multiple peroral administration of a corpuscular pertussis vaccine]. | multiple oral immunization with pertussis corpuscular vaccine was shown to lead to the considerable stimulation of local and systemic humoral immunity. the data on the titers of specific and normal secretory antibodies, on the levels of iga in washings from the oral cavity, the small intestine and the lungs, on the titers of agglutinins and hemagglutinins in the blood serum, as well as on the morpho-functional transformation of the mucous membrane and the associated lymphoid tissue in the digest ... | 1985 | 2868593 |
| [determination of antibodies to streptococcus group a polysaccharide in human sera by an immunoenzyme method]. | use was made of the elisa to develop a highly sensitive quantitative method for detection of antibodies against streptococcus group a polysaccharide (polysaccharide a) in human sera. the main advantage is that one can use only one optimal dilution of the sera together with the reference serum. sera of 53 healthy volunteers and 77 patients with a history of streptococcus group a infections were screened for the presence of polysaccharide a antibodies. highly reproducible results were obtained in ... | 1985 | 2578835 |
| generation of the electrochemical potential of na+ by the na+-motive nadh oxidase in inverted membrane vesicles of vibrio alginolyticus. | inverted membrane vesicles prepared from vibrio alginolyticus generated a membrane potential (positive inside) and accumulated na+ by the oxidation of nadh. generation of the membrane potential required na+ and was inhibited by 2-heptyl-4-hydroxyquinoline n-oxide, a specific inhibitor of the na+-dependent nadh oxidase. collapse of the membrane potential by valinomycin stimulated the uptake of na+. in contrast, accumulation of h+ was not detected under all the conditions tested. these results sug ... | 1985 | 2579856 |
| renal apical membrane cholesterol and fluidity in regulation of phosphate transport. | renal proximal tubule cells adapt to dietary phosphate (pi) restriction by increasing pi transport independent of parathyroid hormone, vitamin d metabolites, or serum ca2+. to determine the underlying cellular mechanism(s), brush border (bbm) and basolateral membranes (blm) were isolated from growing male rats fed a synthetic diet containing variable levels of pi (0.1-1.4%). dietary pi restriction was without effect on either bbm or blm total lipid phosphorus, individual phospholipid species, or ... | 1985 | 3160247 |
| oxidative metabolism of neonatal and adult rabbit lung macrophages stimulated with opsonized group b streptococci. | we compared the oxidative metabolism of alveolar macrophages (am) from adult and neonatal (1- and 7-day-old) rabbits before and after their in vitro exposure to type ia group b streptococci (gbs) opsonized with immune rabbit serum. nonstimulated am from 1-day-old, 7-day-old, or adult rabbits consumed o2 at a rate of 17 to 20 nmol/10(6) am per 10 min under basal conditions and released minimal amounts of superoxide (o2-) into the medium. approximately 80% of this basal respiration was of mitochon ... | 1985 | 2981197 |
| the biologic significance of the mixed lymphocyte kidney culture in humans. | the mixed lymphocyte kidney culture (mlkc) in humans has been studied in normal and abnormal clinical conditions. human renal cortical cells were extracted by collagenase treatment from the kidneys of "normal" heart-beating cadaver organ donors (n = 13), patients with end-stage renal disease (esrd) at pretransplant bilateral nephrectomy and splenectomy (n = 13), and from irreversibly rejected renal allografts at the time of graft nephrectomy (n = 5). proliferation of peripheral blood t lymphocyt ... | 1985 | 2996186 |
| [role of virus-induced autoreactive t-lymphocytes, t-suppressors and the serum factor regulating their activity in the pathogenesis of experimental infection caused by the langat virus in mice]. | the precursors of autoreactive t-lymphocytes (partl) have been detected in the spleen of mice infected with langat virus. when introduced into syngeneic recipients, partl differentiate in their lymph nodes into autoreactive t-lymphocytes (artl) causing a fatal autoimmune disease in the syngeneic recipients in vivo and capable of destroying syngeneic cell cultures in vitro. in the thymus of mice infected with langat virus t-suppressors (ts) inhibiting the differentiation of partl into artl have b ... | 1985 | 2998127 |
| [effect of titanium phosphate, zirconium oxide and a mixture of ammonium phosphotungstenate with zirconium phosphate on the elimination of radioactive cesium from the bodies of rats]. | 1985 | 3835674 | |
| isolation and characterization of neuropeptide y from porcine intestine. | the isolation and primary structure of intestinal neuropeptide y (npy) is described. the peptide was purified from porcine intestinal extracts using a chemical assay and radioimmunoassay for npy. the amino acid sequence of this peptide is: tyr-pro-ser-lys-pro-asp-asn-pro-gly-glu-asp-ala-pro-ala-glu-asp-leu-ala- arg-tyr-tyr- ser-ala-leu-arg-his-tyr-ile-asn-leu-ile-thr-arg-gln-arg-tyr-nh2. this the structure of intestinal npy is identical to the npy of brain origin. | 1985 | 3838090 |
| is raised plasma peptide yy after intestinal resection in the rat responsible for the trophic response? | the relationship between the adaptive response and plasma pyy concentrations after small bowel resection has been investigated. seventy five per cent proximal small bowel resection resulted in a rise in plasma pyy at six days from 28 +/- 3.1 to 85 +/- 12.3 pmol/l (p less than 0.001) and this difference was maintained to 48 days. plasma pyy correlates both with crypt cell production rate (ccpr) in the ileum and with plasma enteroglucagon levels. in a second study, pyy or saline was infused over a ... | 1985 | 3841330 |
| sequence analysis of peptide fragments from the intrinsic membrane protein of calf lens fibers mp26 and its natural maturation product mp22. | calf lens fiber plasma membranes, containing only the intrinsic membrane protein mp26 and its maturation product mp22 were treated with proteolytic enzymes such as trypsin, protease v8 from s. aureus or with chemical agents as cnbr in formic acid. the cleavage products, purified by electrophoresis, were analysed for their amino acid composition and n-terminal sequences. proteolysis gave rise to peptides which were mainly shortened at the c-terminal end of the molecules. while the v8 protease pro ... | 1985 | 3882455 |
| cardioprotective effects of defibrotide in acute myocardial ischemia in the cat. | we investigated the effects of defibrotide (d), a natural polydeoxyribonucleotide, on acute myocardial ischemia (ami) in anesthetized cats. a permanent ligature was placed around the left anterior descending coronary artery (lad) 12-14 mm from its origin. ventricular fibrillation and death were exceptional and when they occurred the cats were not included in the evaluation. pretreatment of cats with d, 32 mg kg-1 h-1, i.v. infusion, maintained throughout the 5 h occlusion period, reduced ami-st ... | 1985 | 3890260 |
| cardioprotective effects of defibrotide in acute myocardial ischemia in the cat. | we investigated the effects of defibrotide (d), a natural polydeoxyribonucleotide, on acute myocardial ischemia (ami) in anesthetized cats. a permanent ligature was placed around the left anterior descending coronary artery (lad) 12-14 mm from its origin. ventricular fibrillation and death were exceptional and when they occurred the cats were not included in the evaluation. pretreatment of cats with d, 32 mg kg-1 h-1, i.v. infusion, maintained throughout the 5 h occlusion period, reduced ami-st ... | 1985 | 3890260 |
| [root submergence: prevention of jaw resorption. review of the literature]. | 1985 | 3909247 | |
| growth of bacteria on chitin, fungal cell walls and fungal biomass, and the effect of extracellular enzymes produced by these cultures on the antifungal activity of amphotericin b. | vibrio alginolyticus, streptomyces griseus, arthrobacter g12, bacillus sp. and cytophaga sp. were grown on solid and liquid media containing soluble and insoluble carbon sources. arthrobacter g12, bacillus sp. and cytophaga sp. grew well on media which contained fungal cell walls or fungal biomass as the main carbon source. all bacteria produced extracellular proteases and all bacteria except arthrobacter g12 produced extracellular chitinases. growth of cytophaga sp. on colloidal chitin was para ... | 1985 | 3929028 |
| effect of melanin concentrating hormone on pigment and adrenal cells in vitro. | highly purified synthetic salmonid melanin concentrating hormone (mch) and some analogs were investigated for their ability to concentrate the pigment in scale melanophores of the chinese grass carp, ctenopharyngodon idellus, to produce melanin dispersion in frog or lizard melanophores and to inhibit alpha-msh in its action on mouse melanoma and rat adrenal glomerulosa cells in vitro. in the grass carp, mch produced half-maximal pigment aggregation at 6 x 10(-11) m and its oxidized form at 7 x 1 ... | 1985 | 3938841 |
| identical chromosome translocations involving the region of the c-myb oncogene in four metastases of a mediastinal teratocarcinoma. | a 39-year-old white male presented with a disseminated mediastinal teratocarcinoma. karyotyping was performed on two mature residual metastatic lesions in the lungs immediately following chemotherapy, on a recurring lung lesion after 5 months, and on a metastasis in the right thigh 5 months after salvage chemotherapy. all four lesions were pseudoeuploid and showed identical chromosomal abnormalities: a translocation with the two chromosomes #6 and one chromosome #11 involved, resulting in 46, xy ... | 1985 | 3967222 |
| lesioning of the rat basal forebrain leads to memory impairments in passive and active avoidance tasks. | effects of the bilateral electrolytic lesioning of the basal forebrain (bf), including the ventral globus pallidus, on passive or active avoidance tasks, were studied in male wistar rats. a severe deficit in acquisition of passive avoidance response was produced by the lesioning in the posterior level of bf. the retention of the passive avoidance response was markedly disrupted with post-training lesioning. time-dependent but only slight recovery from the memory impairments was observed in the p ... | 1985 | 3971182 |
| vibrio alginolyticus conjunctivitis. first reported case. | a gravely ill patient had vibrio alginolyticus conjunctivitis develop, possibly from contact with seashell fragments. it is believed to be the first reported instance of ocular infection by this organism. marine vibrio organisms must be considered as potential sources of ocular infection in patients with direct or indirect exposure to salt water habitats, or in patients who are immunocompromised. | 1985 | 3977694 |
| lateral flagellar antigen of vibrio alginolyticus and vibrio harveyi: existence of serovars common to the two species. | the antigenicity of lateral (l-) flagella of two marine vibrios, vibrio alginolyticus and v. harveyi, was studied, and the two species were found to have common antigenicity of their flagella. antisera against l-flagella were prepared by immunizing rabbits with highly purified l-flagellar filaments. h-agglutination tests with the anti-l-flagella antisera showed that four h-serovars existed in these species and that two of them were shared by the two species. cross reactivity between h-serovars o ... | 1985 | 4010543 |
| effect of stilboestrol on adrenal steroidogenesis in albino rats. | 1985 | 4018847 | |
| sucrose uptake is driven by the na+ electrochemical potential in the marine bacterium vibrio alginolyticus. | na+ was found to be essential for the accumulation of sucrose by vibrio alginolyticus. sucrose uptake was completely inhibited by the addition of proton conductor at neutral ph, but not at alkaline ph, where the primary electrogenic na+ pump generates the na+ electrochemical gradient. we therefore conclude that sucrose transport is driven by the electrochemical potential of na+ in this organism. | 1985 | 4030698 |
| vibrio alginolyticus conjunctivitis. | 1985 | 4038119 | |
| effect of ca2+ on the binding characteristics of muscarinic receptors in rat adenohypophysis--variation during the estrous cycle. | the effect of ca2+ on the biochemical characteristics of muscarinic receptors in the adenohypophysis of male and female rats at the various stages of the estrous cycle was investigated in binding experiments using the specific muscarinic antagonist n-methyl-4-piperidyl benzylate ( [3h]-4nmpb) and the muscarinic agonist oxotremorine. by using ca2+ chelators such as egta, and ca2+ channel blockers such as d-600, we showed that ca2+ profoundly alters the binding characteristics of both antagonists ... | 1985 | 4040223 |
| local cerebral glucose utilization in normal female rats: variations during the estrous cycle and comparison with males. | the quantitative 2-[14c]deoxyglucose autoradiographic method was used to study the fluctuations of energy metabolism in discrete brain regions of female rats during the estrous cycle. a consistent though statistically nonsignificant cyclic variation in average glucose utilization of the brain as a whole was observed. highest levels of glucose utilization occurred during proestrus and metestrus, whereas lower rates were found during estrus and diestrus. statistically significant fluctuations were ... | 1985 | 4040921 |
| [early penicillin treatment can prevent cns symptoms in tick-transmitted borrelia meningitis]. | 1985 | 4046663 | |
| comparison of api 20e, api rapid e, and api rapid nft for identification of members of the family vibrionaceae. | sixty isolates, from nine species of the family vibrionaceae, were tested by the api 20e, api rapid e, and api rapid nft systems. results were compared with those obtained with standard biochemicals. included were 7 aeromonas caviae isolates, 27 aeromonas hydrophilia isolates, 10 aeromonas sobria isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolat ... | 1985 | 4056003 |
| an infection by vibrio alginolyticus in an atlantic bottlenose dolphin housed in an open ocean pen. | 1985 | 4078981 | |
| growth of vibrio costicola and other moderate halophiles in a chemically defined minimal medium. | a simple chemically defined minimal medium consisting of sodium glutamate, glucose, vitamins, and salts was devised to support growth of the moderate halophile, vibrio costicola, over as wide a range of nacl concentrations as the complex medium, proteose peptone + tryptone. the lag period at higher nacl concentrations was longer in the chemically defined minimal medium than in proteose peptone + tryptone. chemically defined minimal medium also supported the growth of an unidentified moderate hal ... | 1985 | 4084863 |
| characterization and distribution of vibrio alginolyticus and vibrio parahaemolyticus isolated in indonesia. | previous studies have shown that vibrio alginolyticus and vibrio parahaemolyticus can be isolated from similar types of marine samples. in this report, the results of an examination of 567 v. alginolyticus and v. parahaemolyticus strains, isolated from seawater in jakarta bay and from more than 30 types of seafood from markets in jakarta, indonesia, are presented. most isolates were from mackerel, shrimp, or squid. numerical taxonomic analyses clustered 337 isolates and three v. alginolyticus re ... | 1985 | 4091566 |
| the ada conference on increasing access to dental care. a report. | 1985 | 268525 | |
| [the implication of renal cortical blood flow detected by 99mtc-dimercaptosuccinic acid (dmsa) renal scintigraphy (author's transl)]. | 1985 | 219279 | |
| different effects of the ionophore a-23187 and d-glucose on 45ca2+ fluxes in isolated islets of ob/ob-mice. | fluxes of 45ca2+ were studied in beta-cell rich islets of non-inbred ob/ob-mice, using lacl3 to wash out extra-cellular and superficially bound 45ca2+. the ionophore a-23187 (10 microm) increased the 45ca2+ uptake in islets both at 3 and 20 mm d-glucose, the effect being more pronounced after 10 min than after 120 min of incubation. in incubations for 120 min, 20 mm d-glucose induced a higher uptake of 45ca2+ than did a-23187. the ionophore enhanced the unidirectional efflux of 45ca2+ from prelo ... | 1986 | 362823 |
| phospholipase a2-treated human high-density lipoprotein and cholesterol movements: exchange processes and lecithin: cholesterol acyltransferase reactivity. | human hdl3 (d 1.125-1.21 g/ml) were treated by an exogenous phospholipase a2 from crotalus adamenteus in the presence of albumin. phosphatidylcholine hydrolysis ranged between 30 and 90% and the reisolated particle was essentially devoid of lipolysis products. (1) an exchange of free cholesterol was recorded between radiolabelled erythrocytes at 5-10% haematocrit and hdl3 (0.6 mm total cholesterol) from 0 to 12-15 h. isotopic equilibration was reached. kinetic analysis of the data indicated a co ... | 1986 | 3942760 |
| an nadh:quinone oxidoreductase of the halotolerant bacterium ba1 is specifically dependent on sodium ions. | the rate of nadh oxidation by inverted membrane vesicles prepared from the halotolerant bacterium ba1 of the dead sea is increased specifically by sodium ions, as observed earlier in whole cells. the site of this sodium effect is identified as the nadh: quinone oxidoreductase, similarly to the other such system known, vibrio alginolyticus (h. tokuda and t. unemoto (1984) j. biol. chem. 259, 7785-7790). sodium accelerates quinone reduction severalfold, but oxidation of the quinol, with oxygen as ... | 1986 | 3947089 |
| specialized contacts between sarcolemma and sarcoplasmic reticulum at the ends of muscle fibers in the diaphragm of the rat. | an electron-microscopic study of the myotendinous portion of the diaphragm in the wistar rat has shown that at the ends of muscle fibers, longitudinally oriented invaginations and peripheral furrows of the sarcolemma establish specialized contacts with individual sacs of the sarcoplasmic reticulum. the construction of these terminal contacts is similar to that of contacts between sarcolemmic t-tubules and terminal cisternae of the sarcoplasmic reticulum, characterized by formation of triads. the ... | 1986 | 3948240 |
| [etiological role of genital mycoplasma in non-gonococcal urethritis and complications]. | 1986 | 3963999 | |
| evidence for the presence of a novel biosynthetic pathway for norspermidine in vibrio. | enzymatic studies of the cell extracts of vibrio alginolyticus and v. parahaemolyticus provided evidence that there exists a novel biosynthetic pathway for norspermidine (nh2(ch2)3nh(ch2)3nh2), a major polyamine species. in this pathway, the schiff base formed between aspartic beta-semialdehyde and 1,3-diaminopropane is first reduced by a nadph-dependent enzyme to yield "carboxynorspermidine" (nh2(ch2)3nh(ch2)2ch(nh2)cooh), which is in turn decarboxylated by a pyridoxal phosphate dependent enzym ... | 1986 | 3697846 |
| intracranial infection by vibrio alginolyticus following injury in salt water. | a 20-year-old man presented with an epidural abscess 3 months after a seawater diving accident. cultures of the abscess cavity obtained by surgical drainage revealed a pure culture of vibrio alginolyticus. marine vibrios may produce serious intracranial infection after head injury in salt water. | 1986 | 3700619 |
| comparison of gastrointestinal ph in dogs and humans: implications on the use of the beagle dog as a model for oral absorption in humans. | gastrointestinal ph as a function of time was recorded for 4 beagle dogs and 10 human subjects using radiotelemetric ph measuring equipment. results indicated that in the quiescent phase, gastric ph in the dogs (mean = 1.8 +/- 0.07 sem) was significantly (p less than 0.05) higher than in humans (1.1 +/- 0.15). no significant difference in the time for the ph monitoring device to empty from the stomach was noted for the two species (99.8 +/- 27.2 min for dogs, 59.7 +/- 14.8 min for humans, p grea ... | 1986 | 3701609 |
| further studies on pu metabolism by swine. | 1986 | 3710797 | |
| sodium translocation by nadh oxidase of vibrio alginolyticus: isolation and characterization of the sodium pump-defective mutants. | 1986 | 3713539 | |
| n-ethylmaleimide desensitizes ph-dependence of k+/h+ antiporter in a marine bacterium, vibrio alginolyticus. | the k+/h+ antiporter of a marine bacterium, vibrio alginolyticus, is strongly dependent upon the cytoplasmic ph and functions only at an internal ph above 7.7. in alkaline buffer with an outwardly directed chemical gradient of k+ (delta pk), the internal ph was maintained at about 7.7. addition of n-ethylmaleimide (nem) released cellular k+ and acidified the cytosol below ph 7.7. the nem effect was reversed by the addition of 2-mercaptoethanol: k+ efflux ceased, and the internal ph returned to a ... | 1986 | 3718495 |
| anticandidal activities of terconazole, a broad-spectrum antimycotic. | terconazole is a new triazole ketal derivative with broad-spectrum in vitro and in vivo antifungal activities. this study further characterizes the effects of terconazole in vitro on yeast cell growth, viability, and morphology. terconazole inhibited the growth of candida albicans atcc 44859 in a concentration-related manner, but with modest effects noted at levels from 10(-8) to 10(-5) m when the yeast was grown on media favoring the cell form. the inhibitory potency of terconazole on yeast cel ... | 1986 | 3729366 |
| purification and initial characterization of the 71-kilodalton rat heat-shock protein and its cognate as fatty acid binding proteins. | the major rat heat-shock (stress) protein and its cognate were purified to electrophoretic homogeneity from livers of heat-shocked rats. both proteins exhibited similar behavior on a variety of column chromatography matrices but were separable by preparative isoelectric focusing under nondenaturing conditions by virtue of a 0.2 ph unit difference in isoelectric point. both purified proteins had similar physical properties, suggesting the possibility that they may have similar biological function ... | 1986 | 3730359 |
| the sodium cycle. iii. vibrio alginolyticus resembles vibrio cholerae and some other vibriones by flagellar motor and ribosomal 5s-rna structures. | an electron microscopic study of the basal bodies of the vibrio albinolyticus flagellum revealed a four-disc structure. the diameters of the two discs localized closer to the cytoplasmic membrane proved to be about 2-fold shorter than those of the two others. in this respect the basal body of v. alginolyticus resembles very much that of v. cholerae described by ferris and co-workers. the sequence of the v. alginolyticus ribosomal 5s-rna showed that it is similar to those of v. cholerae, v. harve ... | 1986 | 3730372 |
| a method for repeated csf sampling in the freely moving rat. | with the use of a holder mounted on the skull of rats, a cannula can be implanted so that one end rests on the atlanto-occipital membrane overlying the cisterna magna. needles introduced through the cannula allow repeated access to the cisterna magna in the freely moving animal. this system is stable for periods of up to 4 weeks and is suited for repeated sampling of the cerebrospinal fluid. | 1986 | 3747592 |
| morphological changes in rat striatal boutons after chronic methamphetamine and haloperidol treatment. | dopaminergic (da) synaptic boutons were identified in rat striatum with an electron microscopic histochemical method. in rats which developed behavioral hypersensitivity after treatment with methamphetamine (map) for about 2 weeks, significantly fewer da boutons were found. this effect was specific to da boutons without mitochondria and was not seen in boutons with mitochondria. the density of granular synaptic vesicles in da boutons, however, did not change significantly. these morphological ch ... | 1986 | 3748472 |
| the chemical and enzymatic oxidation of hematohemin ix. identification of hematobiliverdin ix alpha as the sole product of the enzymatic oxidation. | oxidative cleavage of hematohemin ix in pyridine solution in the presence of ascorbic acid (coupled oxidation), followed by esterification of the products with boron trifluoride/methanol produced the four possible hematobiliverdin dimethyl esters in 11.1% overall yield. transetherifications took place simultaneously with the esterification reaction and resulted in the formation of the dimethyl ester of hematobiliverdin ix gamma 8a,13a-dimethyl ether (1.8%), the dimethyl ester of hematobiliverdin ... | 1986 | 3754208 |
| the respiratory burst oxidase of human neutrophils. further studies of the purified enzyme. | a superoxide-forming oxidase from activated human neutrophil membranes was solubilized by two slightly different methods, then purified by "dye-affinity" chromatography. kinetic studies of the purified preparations gave vmax values of 5-10 mumol of o-2/min/mg of protein, and km values for nadh and nadph that were in reasonable agreement with values determined previously using particulate and crude solubilized preparations of the respiratory burst oxidase. sodium dodecyl sulfate-polyacrylamide ge ... | 1986 | 3759962 |
| migration of fish leucocytes in vitro: the effect of factors which may be involved in mediating inflammation. | plaice (pleuronectes platessa l.) neutrophils were isolated from the kidney on a discontinuous percoll gradient and from the peritoneal cavity at the peak of a glycogen-elicited inflammatory response. the migratory ability of neutrophils was assessed using a 48-well microchemotaxis chamber, with an incubation of 1.5 h at 12 degrees c. the two neutrophil populations showed different responses to n-formylmethionyl-leucyl-phenylalanine (fmlp). whereas kidney neutrophils only showed a significant en ... | 1986 | 3765366 |
| duchenne muscular dystrophy in a girl with an (x;15) translocation. | this is a report of a girl with duchenne muscular dystrophy (dmd) associated with an 46,x,t (x;15) (p21; q 26) chromosome constitution. although in the eight published cases of girls with dmd and a t(x;aut) different autosomes were involved in the translocation, the breakpoint was always at xp21. the present case supports the hypothesis that the dmd gene must be located at xp21. in this study, involvement of the father's chromosomes in the translocation was detected. | 1986 | 3777020 |