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new invasive escherichia coli strain.a new invasive escherichia coli serogroup is described. its o antigen is identical to the o antigen of shigella boydii 3. we propose the designation of e. coli são paulo for this serogroup until the situation of its o antigen is settled.19806989859
[formation of iron-binding metabolites by bacteria of the enterobacteriaceae family].the capacity of enterobacteriaceae strains cultivated in a synthetic medium for the biosynthesis of catechols and hydroxamates has been studied. among the strains under study all strains of the genera salmonella (8 strains), escherichia (102 strains), citrobacter (5 strains), enterobacter (2 strains), serratia (1 strain) synthesize catechols. in the genus shigella (128 strains) all sh. flexneri serovars (79 strains) do not synthesize catechols, other representatives of this genus synthesize iron ...19806998212
faecal excretion of entero-pathogens in a pakistani family returning to the u.k. after a visit to rawalpindi.the family consisted of two parents and five children. while the father remained in cardiff, the mother and all the children visited rawalpindi, pakistan, for 6 weeks to stay with relatives. travel was by flight from heathrow airport to pakistan and by a short road journey to rawalpindi. mrs m. - the mother - as a guest, did no cooking on the holiday. the house which they were living in had a piped water supply, thought to be treated. there was no flush toilet but a commode was available and was ...19807005329
survival of shigellae in soil.the survival of four shigella strains in soil (sh. sonnei, sh. boydii, sh. flexneri and sh. dysenteriae) was studied under various conditions. their survival period was tested in two different types of sterile soil at 18-20 degrees c and in one type of soil at 4 and 37 degrees c. this latter type of soil, after enrichment with casaminoacids or (nh4)2hpo4 was also used for testing again the survival of the strains at 18-20 degrees c. though the initial number of the inoculated microorganisms was ...19807006270
resistance to trimethoprim and other antibiotics in shigellae isolated in the province of ontario.antimicrobial susceptibility determinations for three commonly used and seven seldom used antibacterial drugs against 482 strains of shigellae isolated in ontario during 1977 and 1978 were carried out. resistance to the first-line treatment drugs, ampicillin and tetracycline, occurred in 20.5 and 39.2% of strains, respectively. the emergence of trimethoprim-sulfamethoxazole resistance was noted for the first time in 3.2% of the strains in 1978. all strains retained their susceptibility to gentam ...19807006767
[shigella: microbiological aspects and incidence in our environment]. 19806755638
chemotherapy for solid tumours in adults. 1980273416
imported shigellosis: aerogenic shigella boydii 74 (sachs a 12) in a traveller followed by two cases of laboratory-associated infections.during their holiday in tunesia two members of a family developed severe dysentery which prompted hospitalization of one of them after their return to germany. shigella boydii 4 was isolated from a stool culture. from another, healthy family member an aerogenic, mannitol negative strain of s. boydii 14 (formerly sachs a 12) was isolated which subsequently infected a laboratory technician when she was handling the fecal specimen. a second laboratory infection due to this organism occurred while p ...19817015640
[etiological factors in bacillary dysentery in poland 1965-1979]. 19817041196
increase in drug resistance among shigella dysenteriae, sh flexneri, and sh boydii.two thousand three hundred and seventy strains of shigella dysenteriae, sh flexneri, and sh boydii isolated in england and wales from 1974 to 1978 were tested for resistance to 12 antimicrobial drugs. eighty per cent of strains were resistant to one or more drugs, with sulphonamide resistance occurring most frequently. resistance to streptomycin, tetracycline, ampicillin, and chloramphenicol increased during the period, as did the incidence of multiple resistance. most infections due to sh dysen ...19816790085
structural studies on a carbohydrate chain isolated from the lipopolysaccharide of shigella boydii type 8.the lipopolysaccharide isolated from the cells of shigella boydii type 8 bacteria gave precipitin bands against homologous antisera on ouchterlony plates, whereas the carbohydrate-containing fractions obtained from it did not. one of the fractions was obtained in major proportion and contained 23.5% of sugars. a structure was assigned to the carbohydrate chain in this material by using the results of methylation, periodate oxidation, and deamination studies.19826816467
[a familial case of bacillary dysentery caused by gas producing shigella boydii 14]. 19826818307
confirmation of aerogenic strains of shigella boydii 13 and further study of shigella serotypes by dna relatedness.shigella boydii 13 strains are separable from other shigella and escherichia coli strains on the basis of dna relatedness. from this observation, it was possible to confirm the existence of aerogenic s. boydii 13 strains. dna relatedness studies also showed that strains of e. coli and strains representing all other serotypes of shigella, including provisional strains, belong to the same genetic species.19826752183
[bacillary dysentery - 1980]. 19826755564
[epidemiology and prevention of dysentery]. 19827047217
[use of coagglutination for serotyping shigella dysenteriae and shigella boydii].the coagglutination test was used to identify shigella boydii and shigella dysenteriae. a trial was carried out with 13 native rabbit antisera to s. boydii and 10 antisera to s. dysenteriae, as well as with coagglutinating reagents prepared from these antisera. the use of coagglutinating reagents was shown to ensure the complete specificity of the results, to prevent the adsorption of diagnostic antisera and to decrease their consumption 50 times. the importance of the coagglutination test for t ...19826760638
new provisional serovar of shigella boydii.ten bacterial strains are described that give biochemical reactions characteristic of the genus shigella but do not belong to any of the established or provisional serovars. the strains ferment mannitol and are antigenically indistinguishable. they do not possess any of the group antigens of shigella flexneri, and it is therefore proposed that they be regarded as members of a new provisional serovar of shigella boydii. strain e16553 is designated as the test strain for the new serovar.19826761353
clinical study of dental cements. vi. a study of zinc phosphate, eba-reinforced zinc oxide eugenol and polyacrylic acid cements as luting agents in fixed prostheses.a clinical trial for the final cementation of crowns and bridges with a reinforced zinc oxide and eugenol cement, a polyacrylic acid cement and a zinc phosphate cement was made over a 3-year period. the study involved 441 patients for whom 547 bridges and 162 single restorations were cemented. the patients were recalled at 6-month intervals for the duration of the study and the restorations were examined for looseness. of the 547 bridges 520 remained firmly cemented to the abutment teeth. of 1,0 ...1982272385
[bacterial antigenic polysaccharides. 12. structure and 13c nmr spectrum of the polysaccharide chain of shigella boydii type 8 lipopolysaccharide].a specific acidic polysaccharide was isolated from sh. boydii type 8 antigenic lipopolysaccharide after mild hydrolysis followed by chromatography on sephadex g-50. the polysaccharide consists of d-glucuronic acid, d-galacturonic acid, 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-galactose and 2-amino-1,3-propanediol residues in 1:1:1:1:1 ratio. from the results of methylation analysis, partial acid hydrolysis and smith degradation, the structure of the repeating unit of the specific pol ...19836207837
cell-wall lipopolysaccharide of escherichia coli 0114:h2. structure of the polysaccharide chain.the o-specific polysaccharide of the 0114 antigen (lipopolysaccharide) of escherichia coli 0114 and oligosaccharides obtained from it by smith degradation and hydrogen fluoride solvolysis were analyzed, using proton and 13c nuclear magnetic resonance spectroscopy and methylation. the results indicated that the 0114 polysaccharide has the tetrasaccharide repeating unit alpha-n-acetylglucosamine(1 leads to 4) beta-3,6-dideoxy-3-(n-acetyl-l-seryl)aminoglucose(1 leads to 3) beta-ribofuranose(1 leads ...19836309515
enterotoxigenic and enteroinvasive escherichia coli strains belonging to a new o group, o167.a new escherichia coli o group, o167, is described. its o antigen is related to that of shigella boydii 3. a total of 29 strains belonging to the new o group were examined. three strains were determined to be invasive by the sereny test and by tissue culture tests and were non-enterotoxigenic. all three were nonmotile and failed to decarboxylate lysine. a total of 26 strains were noninvasive. of these, 25 were motile, 24 decarboxylated lysine, and 19 were enterotoxigenic, 17 producing heat-stabl ...19836341404
[epidemiology of bacillary dysentery in algeria. i. the epidemiological aspects of dysentery in algeria].bacterial dysentery plays an important role among infectious diseases in algeria. a tendency to the growth of the morbidity rate has been observed. dysentery is irregularly spread in different zones of the country. the highest morbidity rate is registered in the sahara zone and in the eastern part of the country. the morbidity rate among the urban population is higher than among the rural population. s. flexneri prevail in the etiological structure of dysentery infection. of all age groups, the ...19836342313
[trends in the spread of boyd 4 dysentery and the characteristics of its clinical picture and of the biology of its causative agent].the data on the proportion of s. boydii 4 in the general structure of the causative agents of dysentery, as well as in the intraspecific structure of s. boydii, in some areas of the ussr in 1977-1981 are presented. s. boydii strains 4, circulating in one of the areas where their proportion considerably increased in 1980-1981, corresponded to their taxonomic position in their biological properties, while forming a single biochemical variant according to the character of the fermentation of sorbit ...19836356722
molecular comparison of virulence plasmids in shigella and enteroinvasive escherichia coli.virulent isolates of shigella dysenteriae and shigella boydii harboured a 140 mdal plasmid which was either absent or deleted in spontaneously avirulent strains. together with previous data concerning s. sonnei, s. flexneri and enteroinvasive escherichia coli, the present results established the general role of extrachromosomal elements in the virulence of such enteroinvasive species. among different species, these virulence plasmids showed unrelated endonuclease cleavage patterns, whereas hybri ...19836357023
somatic antigens of shigella: the structure of the polysaccharide chain of shigella boydii type 2 lipopolysaccharide.the structure of the polysaccharide chain of shigella boydii type 2 lipopolysaccharide was established using mainly 13c-n.m.r. spectroscopy, partial hydrolysis, smith degradation, and methylation analysis. the repeating unit of the polysaccharide was concluded to be a branched hexasaccharide, as follows: (formula in text). acetaldehyde was detected in the hyrolysate of the lipopolysaccharide, but no evidence was obtained to indicate that acetaldehyde is located in the polysaccharide moiety.19836362879
[shigellosis abroad]. 19836379992
[bacillary dysentery 1982]. 19846390523
[clinical and treatment characteristics of acute dysentery caused by shigella boydii and newcastle]. 19846390958
[conditions and factors determining the etiological structure of dysentery and its alterations. ii. concepts, hypotheses and points of view yesterday and today]. 19846391040
[species of shigella isolated in a 4-year period and antimicrobial sensitivity]. 19846395307
[structure of the causative agents of shigellosis in areas of the ussr 1979-1981].summarized data on the specific structure of shigella strains isolated in different territories of the ussr in 1979-1981 are presented. these data indicate that in the intra-specific structure of s. flexneri strains the proportion of serological subvariant 2a increased and that of serological variant 6 and subvariants 16 and 4a decreased during this period. using one of the base territories of the all-union shigellosis center as an example, the spread of an unusual serological variant of s. flex ...19846395590
drug resistance in shigella dysenteriae, s flexneri and s boydii in england and wales: increasing incidence of resistance to trimethoprim.a total of 2753 strains of shigella belonging to subgroups a, b, and c that were isolated from patients in england and wales during the period from 1979 to mid-1983 were studied. of these, 1690 (61%) were from patients recently returned from abroad or in contact with recent travellers, and 760 (45%) of these affected travellers from the indian subcontinent. the number of strains resistant to sulphonamides and streptomycin remained at a high level throughout (average 76% and 72% respectively). re ...19846423079
sialic acids of a new type from the lipopolysaccharides of pseudomonas aeruginosa and shigella boydii. 19846437679
[a strain of shigella boydii serovar 18 isolated in japan from a diarrhea patient after a trip to southeast asia]. 19846442723
shigella dysentery in tripoli, libya. 19846371260
an outbreak of shigellosis in an ultra-orthodox jewish community.an outbreak of shigellosis due to shigella sonnei, s. flexnerii and s. boydii in an ultra-religious jewish community in southern israel is described. the source of epidemic was traced to vegetables bought from a single source. the importance of epidemic and endemic modes of spread of shigella is discussed.19846379888
[clinical characteristics of dysentery in relation to the oxidation-reduction features of isolated shigella]. 19846379895
effects of saliva and alpha-amylase on antibiotic sensitivity of bacteria.two hundred and ninety-six bacterial isolates were investigated for the effects of saliva and alpha-amylase on their susceptibility to ampicillin, tetracycline, chloramphenicol and gentamicin. when the test organisms were primed with normal and 'diseased' saliva there were no observable differences in the mics of ampicillin and chloramphenicol for group-a streptococci, but alpha-amylase significantly reduced the mic of tetracycline from 2 to 0.25 mg/1. with staphyloccus aureus, priming with sali ...19846087634
percutaneous transluminal coronary angioplasty. 1985373562
structural analysis of the o-glycosidically linked core-region oligosaccharides of human meconium glycoproteins which express oncofoetal antigens.glycoproteins were extracted from meconium samples of group o neonates of secretor type by pronase digestion followed by precipitation in 67% aqueous ethanol and separated into ii antigen enriched and depleted fractions by affinity chromatography. the latter fraction strongly expressed the oncofoetal antigens recognised by natural antibodies in mouse sera and the hybridoma antibody fc 10.2, and this activity was enhanced after mild acid hydrolysis to remove sialic acid and fucose residues. oligo ...19852580706
immunocytochemical studies of somatostatin neurons in brain.immunohistochemical studies with antisera to somatostatin have, in many instances, led the way to our present understanding of the peptidergic nervous system. somatostatin was among the first of the hypophysiotropic hormones shown to be contained in diverse neuronal circuits outside of the hypothalamus. for example, somatostatin is found within neurons ranging in location from the cerebral cortex to primary sensory neurons to enteric neurons within the gut wall. somatostatin was also the first n ...19852863929
the biologic significance of the mixed lymphocyte kidney culture in humans.the mixed lymphocyte kidney culture (mlkc) in humans has been studied in normal and abnormal clinical conditions. human renal cortical cells were extracted by collagenase treatment from the kidneys of "normal" heart-beating cadaver organ donors (n = 13), patients with end-stage renal disease (esrd) at pretransplant bilateral nephrectomy and splenectomy (n = 13), and from irreversibly rejected renal allografts at the time of graft nephrectomy (n = 5). proliferation of peripheral blood t lymphocyt ...19852996186
protein loss of bovine dental enamel during in-vitro subsurface demineralization.a chemical system based on the dialysis principle was used to study protein loss of dental enamel during demineralization with an acetic-acid buffer solution containing calcium and phosphate, in which the fluoride-ion activity was kept constant. this resulted in a subsurface lesion, with a depth of about 130 microns. after demineralization, protein material was isolated from the demineralization solution. u.v. spectra of the protein showed strong absorbance between 240 and 300 nm. amino-acid com ...19853865639
role of the posterior capsule in the prevention of postoperative bacterial endophthalmitis: experimental primate studies and clinical implications.the posterior capsule has an important effect on the risk of postoperative bacterial endophthalmitis. in order to investigate whether the posterior capsule inhibited the spread of infection into the vitreous we performed extracapsular cataract extraction in both eyes of 10 primates. in one eye of each primate the posterior capsule was left intact and in the other eye a large posterior capsulectomy was performed. when the anterior chambers were challenged with equivalent inocula of staphylococcus ...19853877524
addition of three new serotypes of shigella boydii to the shigella schema.no new serotypes have been added to the shigella schema since 1958, although several provisional serotypes have been described. we conducted biochemical and serological studies on three provisional shigella boydii serotypes. four strains of serotype 2710-54 from four widely separated countries, 7 strains of serotype 3615-53 from three different countries, and 31 strains of serotype 1344-78 (e10163) from six different countries were included. reactions of all three serotypes were consistent with ...19853881469
plaque formation by virulent shigella flexneri.an in vitro tissue culture plaque assay was developed to investigate the intracellular replication and intercellular spread of virulent shigellae. shigella plaques were formed in hela cell monolayers in the presence of an agarose overlay containing tissue culture medium and gentamicin, which eliminated extracellular bacterial growth. microscopically, the plaques were characterized by a central area of dead host cells surrounded by cells infected with shigellae. cells further away from the plaque ...19853884506
studies of metabolites in diarrheal stool specimens containing shigella species by frequency-pulsed electron capture gas-liquid chromatography.eleven diarrheal stool specimens and 10 control stool specimens from cairo, egypt, were studied by frequency-pulsed electron capture gas-liquid chromatography (fpec-glc). four cases involving shigella sonnei, three cases involving shigella boydii, and four cases involving shigella flexneri were studied. the aqueous stools were centrifuged, extracted with organic solvents, and derivatized to form specific electron-capturing derivatives of carboxylic acids, alcohols, hydroxy acids, and amines. ana ...19853886695
[shigella serotypes isolated july-october 1983 and their antibiotic sensitivity].twenty four strains of shigella were isolated from the stool of patients who had been admitted to the hospital with the complaint of diarrhea. of these, 17 were found to be sh. flexneri, and 7 sh. boydii. antibiotic susceptibility tests indicated that all of the 24 isolates probably had multiple resistance factors. twenty three of 24 strains were found to be resistant to ampicillin. this may be important from a therapeutic point of view.19853892249
[detection of shigella antigens in the feces of patients with acute intestinal diseases using a coagglutination reaction].a total of 113 patients with acute intestinal diseases have been examined with the use of the coagglutination test. 84.95% of the patients showed the presence of different shigella antigens. in patients with bacteriologically confirmed dysentery the corresponding shigella antigens were detected in 96.97% of cases in s. sonnei dysentery, in 90% of cases in s. flexneri dysentery, in 75% of cases in s. newcastle dysentery and in 100% of bases in s. boydii dysentery. in 81.6% of patients with acute ...19853892986
epidemiology of shigella boydii serotype 2, a strain indigenous to spain. 19853899639
shigella septicemia in elderly patients. 19853899990
[3-year study of shigellosis epidemic in rwanda, central africa. problems of public health and bacteriological aspects].since 1979, the lakes region (kivu in zaïre, western uganda, rwanda, burundi) has been stricken by a bacillary dysentery epidemic at s. dysenteriae type 1. supposed to be extinct since the 1950s., this bacillus has revealed a very noticeable virulence as well by the number of registered cases (524/100.000 in 1983 at ruhengeri-rwanda) as its fast break through. demographic density (from 400 to 600/km2) has played an important role and has facilitated the transition toward epidemicity. systematic ...19853906345
determination of nanogram quantities of vanadium in biological material by isotope dilution thermal ionization mass spectrometry with ion counting detection. 19853907412
n-methyl-l-glutamate synthase. 19853911004
mechanism of sbcb-suppression of the recbc-deficiency in postreplication repair in uv-irradiated escherichia coli k-12.the mechanism by which an sbcb mutation suppresses the deficiency in postreplication repair shown by recb recc mutants of escherichia coli was studied. the presence of an sbcb mutation in uvra recb recc cells increased their resistance to uv radiation. this enhanced resistance was not due to a suppression of the minor deficiency in the repair of dna daughter-strand gaps or to an inhibition of the production of dna double-strand breaks in uv-irradiated uvra recb recc cells; rather, the presence o ...19853911022
photochemical modifications of lac repressor: effect of effectors binding on tryptophan photooxidation. 19853911222
[significance of isolating shigella under epidemiological circumstances]. 19853911318
bactericidal properties of a new water disinfectant.the n-chloramine compound 3-chloro-4,4-dimethyl-2-oxazolidinone (agent i) has been compared with calcium hypochlorite as to its efficacy as a bactericide for the treatment of water. the study included concentration, contact time, ph, temperature, and water quality as controlled variables. the species of bacteria tested were staphylococcus aureus, pseudomonas aeruginosa, and shigella boydii. in general, for highly pure, demand-free water, calcium hypochlorite was the more rapid disinfectant at a ...19853922300
studies on microsomal azoreduction. n,n-dimethyl-4-aminoazobenzene (dab) and its derivatives.the azoreduction of n,n-dimethyl-4-aminoazobenzene (dab) and n-methyl-4-amino-azobenzene (mab) by rat liver microsomes was investigated. it was shown that measurement of azoreduction of dab and structurally related azo dyes by the conventional method of substrate disappearance required an anaerobic environment since n-demethylated and ring-hydroxylated metabolites formed aerobically interfered with the assay system, producing quantitatively inaccurate results. oxygen partially, but not totally, ...19853929790
identification of 5-acetamido-3,5,7,9-tetradeoxy-7-[(r)-3-hydroxybutyramido]-l- glycero-l-manno-nonulosonic acid as a component of bacterial polysaccharides. 19853933831
initiation and promotion in young and old animals. implications for human tumour formation.the significance of ageing in experimental skin carcinogenesis and on the manifestation of tumour formation in man was studied. initiation with the carcinogenic hydrocarbon 7,12-dimethylbenz [a]anthracene and promotion with croton oil in mouse skin showed that tumour formation was unchanged when promotion was delayed 3, 10, 23 or 43 weeks in comparison with that in untreated animals of the same age. the sensitivity to carcinogen treatment of animals over 71 weeks of age was less than that of ani ...19853938448
structural identification of platelet activating factor in psoriatic scale.platelet activating factor was isolated from the lesional scale of psoriatic patients using the method described by bligh and dyer (8). the extract was subjected to thin layer chromatography, and the region of the plate co-migrating with platelet activating factor removed. a portion of each sample was assayed for aggregating activity using washed guinea-pig platelets and the remainder treated with phospholipase c, derivatised, and subjected to reversed phase high performance liquid chromatograph ...19853970690
spatial contrast sensitivity deficits in monkeys produced by optically induced anisometropia.an anisometropia was simulated in infant rhesus monkeys by securing a high-powered minus lens (-10 d) in front of one eye. the anisometropia rearing procedure was initiated at 30 days of age and was continued for durations of 30, 60, or 90 days. behavioral measurements of spatial contrast sensitivity obtained when the animals were 9 months of age indicated that the monkeys treated for 30 days had equal or nearly equal contrast sensitivities and cut-off spatial frequencies in the two eyes. the 30 ...19853972513
noninvasive measurement of pressure/volume characteristics in human limb veins by electrical admittance plethysmography. 19853974317
[persistent chlamydia infection in cell culture]. 19853993192
[mechanism of the anti-arrhythmic action of phosphocreatine in acute myocardial ischemia].the association between phosphocreatine's antifibrillatory action and its effect on the excitement propagation processes in the ischemic area was investigated under acute coronary arterial occlusion in dogs. ischemia considerably reduced the amplitude, and increased the duration and time of onset, in local electrograms, and provoked cardiac fibrillation at the time of occlusion or during the recovery of coronary flow. a single intravenous injection of 300 mg/kg phosphocreatine eliminated cardiac ...19854021280
[ultrastructural aspects of the wound healing process of the skin of albino rats]. 19854089424
the anatomy of a smile.the anatomy of the smile is an integral part of dentistry. its understanding involves close scrutiny of all elements of the oral region. it is not enough to establish the size of teeth based on the high and low lip lines, size of the mouth, and a shade to blend with the age and complexion. to create a harmonious smile the dentist must maintain or create the normal curvature of the lips, proper exposure of the red zone of the lips, an undistorted philtrum, and undisturbed nasolabial grooves. thes ...1985271728
[effects of different postures on left ventricular function during various trimesters of pregnancy]. 1985261924
[evaluation of various radiographic technics for the diagnosis of periodontal diseases with special reference to the status x unilateral technic].various x-ray techniques (panorama views and intraoral plates) were contrasted and evaluated for diagnostic use in periodontal lesions. in addition the status x-unilateral technique (durner, meyer) was compared to intraoral right angle roentgen states (updergrave) in the examination of 20 patients.1985271590
comparison of minimum inhibitory concentration of water soluble extracts of eugenia jambolana lam. (fam. myrtaceae) barks of different ages on dysentery and diarrhoea forming micro - organisms.a preliminary investigations was carried out to study the antibacterial activity of the water soluble extracts of five and ten years old barks of eugenia jambolana lam. (fam. myrtaceae) on dysentery and diarrhoea forming micro organisms. it was observed that the barks of young plants have a better inhibitory effect on micro - organisms like salmonella viballerup, shigella dysenteriae 10, shigella boydii 5, sgigella dysenteriae 2.198522557509
somatotopic organization of motoneurons innervating the pronators, carpal and digital flexors and forepaw muscles in the dog: a retrograde horseradish peroxidase study.central distribution of motor neurons innervating the individual forearm and forepaw muscle through the median and ulnar nerves was studied in the dog using the retrograde horseradish peroxidase (hrp) method. all hrp-labeled cells were seen ipsilaterally in restricted parts within the dorsolateral (dl) and retrodorsolateral nucleus (rdl) of the ventral horn at levels from the cranial tip of c7 to the cranial third of t2. the results showed that motoneurons situated more cranially supply the more ...19863708348
[fasciculation of the median nerve in adults and in the human fetus]. 19863726653
establishment of passive hemagglutination assay (pha) system for anti-hbc in plasma.a sensitive and specific assay system for anti-hbc, the passive hemagglutination (pha) method, has been established. the reactivity of pha cells prepared by conjugating purified recombinant hbcag-particles with fixed sheep blood cells (srbc) was highly specific to monoclonal- and polyclonal anti-hbc iggs. the sensitivity of pha method was higher than that of radioimmunoassay (ria). however, uncertainty for the positivity of anti-hbc still remained in plasma with the pha titers lower than 2(5). a ...19863738912
1,25-dihydroxyvitamin d3 treatment results in increased choline acetyltransferase activity in specific brain nuclei.to better understand the role of 1,25-dihydroxyvitamin d3 [1,25-(oh)2d3] in brain function, the level of calcium-binding protein (cabp) and the activities of choline acetyltransferase (cat) and monoamine oxidase were measured in discrete brain nuclei of vitamin d-deficient and -replete male rats. the nuclei sampled were those in which receptors for 1,25-(oh)2d3 and/or vitamin d-dependent cabp have been localized. significant elevations in cat activity were found in the arcuatemedian eminence and ...19863753932
[establishment of a spontaneously transformed human fetal lung cell line and its biological characteristics].a spontaneously transformed human fetal lung cell line (hflt) was derived from a human fetal lung diploid fibroblast cell line (2bs) by continuous culture. the biological characteristics of this cell line were studied and compared with those of 2bs cells. accompanying the morphological alteration, the growth rate of the transformed cell was accelerated and the maximum cell density was increased. the anchorage-independent growth was shown by its ability to form colonies in soft agar. the collagen ...19863769747
laser-assisted vasal anastomosis in the rat and man.in order to study the efficacy of laser-assisted vasal anastomosis (lava), a microscopic carbon dioxide surgical laser (xanar) was utilized in the anastomoses of human vas deferens in vitro and the sprague-dawley rat vas deferens in vivo. the longitudinal tensile and internal hydrostatic pressure strengths of laser-assisted vasal anastomoses were compared to conventional microsurgical suture anastomoses in the human vas deferens. the lava group had a greater mean internal hydrostatic pressure st ...19863773082
[an attempt to create a factorial model of the epizootic process in sheep infected with s. abortus ovis].experiments were carried out to build up a factorial model with a group of sheep at different age in an infected region. studied were the aspects of the disease as caused by salmonella abortus ovis alone and in combination with chl. ps. var. ovis. it was found that the factorial model can explain sufficiently well (77.22 per cent) the discussed system--the epizootic process with year-old females and sheep affected with pure and mixed infection. the epizootic process with a mixed infection in the ...19863811206
a follow up of t-cell subsets and of anti-m. leprae antibody titer as measured by the fla-abs test in melanesian leprosy patients under polychemotherapy.melanesian leprosy patients from new caledonia were studied for the following parameters during the course of polychemotherapy: peripheral blood t-cell subsets, as identified in an immunofluorescence assay with monoclonal antibodies okt3 ("pan-t"), okt4 ("helper/inducer"), and okt8 ("cytotoxic-suppressor"), and anti-mycobacterium leprae antibodies in the serum, as measured by the fluorescent leprosy antibody absorption test. a group of melanesian healthy subjects with no known exposure to m. lep ...19862940306
dose-related effects of synthetic human beta-endorphin and naloxone on fed gastrointestinal motility.in humans, plasma beta-endorphin levels rise during application of acute stressful stimuli (vertigo, cold pain, and transcutaneous electrical stimulation) that induce gut motor disturbances. whereas it is possible that circulating beta-endorphin participates in the mediation of these central effects on gut motility, its role cannot be established solely on the basis of changes in plasma levels. therefore, we designed the present study to investigate 1) the dose-related effects of intravenous syn ...19862942039
structure-activity relationships of sex steroid analogs determined in vitro in a thyroid hormone-responsive membrane ca2+-atpase model.physiological concentrations of l-t4 were found previously to stimulate ca2+-atpase activity in vitro in reticulocyte membranes from female rabbits and to inhibit this enzyme in reticulocyte membranes from males. in these previous studies, preincubation of intact cells or ghosts with testosterone (5 x 10(-11) m) converted female-source reticulocyte membranes to male-type responsiveness to thyroid hormone (inhibition of ca2+-atpase activity). preincubation of reticulocyte membranes with 17 beta-e ...19862946570
structure-activity relationships of sex steroid analogs determined in vitro in a thyroid hormone-responsive membrane ca2+-atpase model.physiological concentrations of l-t4 were found previously to stimulate ca2+-atpase activity in vitro in reticulocyte membranes from female rabbits and to inhibit this enzyme in reticulocyte membranes from males. in these previous studies, preincubation of intact cells or ghosts with testosterone (5 x 10(-11) m) converted female-source reticulocyte membranes to male-type responsiveness to thyroid hormone (inhibition of ca2+-atpase activity). preincubation of reticulocyte membranes with 17 beta-e ...19862946570
on dying more than one death.death (with a capital d) can best be understood as a series of distinct but related deaths. for example, a pregnant woman was found to be brain-dead but her vital functions were artificially sustained for nine weeks until her fetus could be delivered, after which the machines were removed and she died a second, conceptually distinct death. this procedure is probably justifiable, but any legislation or policy regarding such cases should be flexible and should require consent.19863957650
[development of the classification of bacteria in the genus shigella]. 19863518304
the relation between production of cytotoxin and clinical features in shigellosis.the relation between in vitro production of hela cell cytotoxin by strains of shigella and clinical symptomatology was determined for 35 travelers from the united states who developed shigellosis in guadalajara, mexico. there were 25 patients with shigella sonnei, eight with shigella flexneri, one with shigella boydii, and one with shigella dysenteriae. these strains were evaluated for in vitro production of cytotoxin. the amount of cytotoxin did not correlate with the number of stools passed, t ...19863519788
[conjugative r-plasmids simultaneously belonging to the 2 incompatibility groups inc i zeta and inc b].five plasmids detected in the clinical strains of shigella were studied. the plasmids had similar phenotypes of antibiotic resistance and allotted streptomycin and tetracycline resistance to the host cell. none of them allotted the bacterial cells with sensitivity to phages specific for the inc groups f, n, p and i. the molecular weights of the plasmids ranged from 47 to 50 md. the studies on the incompatibility showed that pkmr 227, pkmr 228-1, pkmr 231, pkmr 242-1 and pkmr 245 plasmids simulta ...19863521469
comparison of dna probes and the sereny test for identification of invasive shigella and escherichia coli strains.forty-two shigella and 29 escherichia coli strains were screened for invasiveness in the sereny test and for hybridization with two recently described dna probes for the invasiveness plasmid. both probes produced identical results. all sereny-positive strains hybridized with both dna probes. three sereny-negative strains also hybridized with the probes, suggesting that there are strains containing the invasiveness plasmid that are not pathogenic in animal models.19863531233
production of shiga toxin and other cytotoxins by serogroups of shigella.we measured the cytotoxic activity of 119 strains of shigella by using a quantitative [3h]thymidine-labeled hela cell assay. we assayed 13 strains of shigella dysenteriae 1; 18 strains of s. dysenteriae types 2 and 3; and 88 strains of shigella sonnei, shigella flexneri, and shigella boydii. strains of s. dysenteriae 1 demonstrated high levels of cytotoxicity (geometric mean, 10(5.04) cd50/mg of protein; range, 10(3.95)-10(6.10). cytotoxic activities of the non-type 1 strains of s. dysenteriae a ...19863537154
shigella from ovine faeces. 19863541356
isotope exchange at equilibrium studies with rat muscle adenylosuccinate synthetase.the kinetic mechanism of rat muscle adenylosuccinate synthetase was studied by determining the rates of isotope exchange at equilibrium. a random sequential binding mechanism was indicated for both the forward and reverse reactions. aspartate, adenylosuccinate, gdp, and pi were determined to bind in rapid equilibrium. gtp exchanges with both gdp and pi at the same rate, which is similar to the exchange rate of imp with adenylosuccinate. aspartate exchanges with adenylosuccinate at a higher rate ...19863542024
[bacteriological study of shigellosis in the lake kivu area (central africa). developments in the last 15 years (1968-1983)].1913 shigella strains were isolated from 1968 to 1983 in lake kivu area (central africa). distribution and evolution of serotypes, biotypes and antibiotypes were studied. the majority of serotypes and biotypes were stable. however, few of these strains showed variations, among them shigella dysenteriae serotype 1, causing epidemic dysenteriae since 1981. epidemiologic survey of shigellosis in kivu seems to be rather underdeveloped (4 bacteriology laboratories for an area of 10 million inhabitant ...19863542256
[beef-extract agar with sodium nucleinate as a medium for storage and stabilization of biological properties of shigella]. 19863078175
occurrence of small hsd plasmids in salmonella typhi, shigella boydii, and escherichia coli.the natural occurrence of small hsd (host specificity for dna) plasmids was demonstrated in restriction endonuclease-producing strains of salmonella typhi, shigella boydii, and escherichia coli. the five hsd plasmids isolated were between 5.0 and 12.2 kilobases long. the copy number of all the hsd plasmids was high (more than 10 copies per cell). introduction of these small plasmids into e. coli strain 0 drastically lowered the efficiency of plating of the lambda.0 phages (the efficiency of plat ...19863003023
differential effects of transforming growth factor type beta on the growth and function of adrenocortical cells in vitro.transforming growth factor type beta (tgf-beta) suppresses basal as well as corticotropin (acth)-stimulated steroid formation by bovine adrenocortical cells in culture. the effect is dose dependent and is not accompanied by any change in adrenocortical cell growth. the minimum effective dose of tgf-beta is 4 x 10(-13) m (10 pg/ml), and maximal inhibition is observed at a concentration of 4 x 10(-11) m (1 ng/ml). a 16- to 20-hr incubation with tgf-beta is required to decrease steroidogenesis, and ...19863020557
differential effects of transforming growth factor type beta on the growth and function of adrenocortical cells in vitro.transforming growth factor type beta (tgf-beta) suppresses basal as well as corticotropin (acth)-stimulated steroid formation by bovine adrenocortical cells in culture. the effect is dose dependent and is not accompanied by any change in adrenocortical cell growth. the minimum effective dose of tgf-beta is 4 x 10(-13) m (10 pg/ml), and maximal inhibition is observed at a concentration of 4 x 10(-11) m (1 ng/ml). a 16- to 20-hr incubation with tgf-beta is required to decrease steroidogenesis, and ...19863020557
exposure of sheep to natural aerosols of foot-and-mouth disease virus.sheep taken individually and allowed to inhale air being drawn along a duct from a cabinet containing pigs acutely infected with foot-and-mouth disease virus for 10 or 15 minute periods were infected by doses as low as 10 tcid50 of virus. the most consistent and reliable indicators of infection were viraemia and seroconversion. the mean times from exposure to onset of viraemia, pyrexia and the appearance of vesicular lesions were 2.5, 3.8 and 4.7 days, respectively. neither the time from exposur ...19863020658
production of restriction endonucleases using multicopy hsd plasmids occurring naturally in pathogenic escherichia coli and shigella boydii.a convenient procedure has been devised for detection of restriction endonucleases in the escherichia coli-shigella group. with this procedure, two restriction endonucleases, designated sbo 13 and eco t22, were found and later were identified as isoschizomers of nrui and avaiii, respectively. these endonucleases were shown to be produced from small multicopy plasmids. they were isolated from nonpathogenic e. coli into which the plasmids had been introduced by transformation, and purified from co ...19863021586
relative activities of 5'-nucleotidase and adenosine deaminase in atrial and ventricular myocardium--the enzyme paradox.the 5'-nucleotidase and adenosine deaminase activities were determined for tissue extracts of up to 10 defined regions of the normal dog heart. the specific activity of 5'-nucleotidase was very significantly higher in atrial than in ventricular myocardium (4 x) and was also higher in right than in left sites of the heart (2 x). the reason for the markedly higher specific activity in atrial muscle is not clear and appears paradoxical relative to adenine nucleotide content and pattern of atp decay ...19863025011
excitatory amino acid transmitters in neuronal circuits of the cat visual cortex.to test a possibility that glutamate (glu) and aspartate (asp) are transmitters in the visual cortex and to locate their operating sites in the cortical circuitry, we studied effects of microiontophoretic application of glu/asp antagonists on visual responses of cortical neurons in the cat. the antagonists tested were kynurenic acid (kyna), cis-2,3-piperidine dicarboxylic acid, and gamma-d-glutamylglycine. among these antagonists, kyna was most effective in blocking visual responses of cortical ...19862870137
integrating treatments in schizophrenia.schizophrenia is a common and severe illness found throughout the world. onset is usually in adolescence and young adulthood, and many individuals have illness-related impairments for life. schizophrenia assaults the very essence of what is distinctly human. the personality is devastated, subjective experience and thought distorted, and the psychological building blocks of everyday life torn asunder. this article discusses diagnostic and conceptual considerations, pharmacotherapy, and interperso ...19862870478
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