Publications

effective method for activity assay of lipase from chromobacterium viscosum.	a method was devised for activity assay of the lipase [triacylglycerol acyl-hydrolase, ec 3.1.1.3] excreted from chromobacterium viscosum into the culture medium; olive oil emulsified with the aid of adekatol 45-s-8 (a non-ionic detergent, the ethoxylate of linear sec-alcohols having chain lengths of 10--16 carbon atoms) was used as the substrate. this method was specifically effective for chromobacterium lipase acitvity assay, and was approximately twice as sensitive as the conventional method, ...	1976	1002673
[microbiological studies of the wanda lake (antarctica)].	the deep-water (68 m) wanda lake in the antarctica is noticeable by three characteristics: a relatively high temperature in the bottom layer, elevated salinity, and the presence of h2s. only several (less often, dozens) saprophytic microorganisms per 40 ml of water are encountered in the lake. the total number of bacteria varies from 6-10(3) to 172-10(3) cells/ml. the highest content of the total bacterial population, and saprophytes, is found in intermediate layers, 30 and 40-50 m deep, respect ...	1976	1012048
immunological relationships of bacterial l-asparaginases.	rabbit antisera against l-asparaginase preparations from escherichia coli, erwinia carotovora, citrobacter sp. and chromobacterium violaceum showed on immunoelectrophoresis that only the enzymes from e. coli and citrobacter are immunologically related. purified preparations had to be used to determine the immunological cross-reactions. immunoelectrophoresis at different ph values yielded the zero mobility points of the enzymes. the activity of the er. carotovora preparation was enhanced up to fo ...	1976	54404
antibodies to bacterial l-asparaginases.	complement fixation has been shown to occur when asparaginase reacts with specific antibody. this is as a result of an antigenic determinant common to the four bacterial asparaginases. patients on asparaginase therapy may produce antibodies and these could be demonstrated by complement fixation.	1977	75527
the chemical structure of the lipid a component of lipopolysaccharides from chromobacterium violaceum nctc 9694.	the chemical structure of the lipid a component of lipopolysaccharides from chromobacterium violaceum nctc9694 was studied. sequential treatment of lipopolysaccharide with alkali, acid, sodium borohydride and hydrazine allowed the isolation of a reduced glucosamine disaccharide. according to methylation studies and enzymic analysis with beta-n-acetylglucosaminidase the d-glucosamine residues are beta(1 leads to 6) linked. the disaccharide carries two phosphate groups, one being linked glycosidic ...	1977	862618
pathogenicity of nonpigmented cultures of chromobacterium violaceum.	nonpigmented cultures of chromobacterium violaceum have been found to be similar to pigmented cultures in their virulence for mice and the pathology of their infections. clinicians and microbiologists should be prepared to consider nonpigmented c. violaceum in their differential diagnoses of infections caused by gram-negative bacteria. the laboratorian who is not aware of this possibility is likely to erroneously identify nonpigmented strains of c. violaceum as members of closely associated gene ...	1977	874071
group ve-2 (chromobacterium typhiflavum) bacteremia.	group ve-2 (chromobacterium typhiflavum) bacteremia occurred in a severely traumatized, post-neurosurgical patient. a discussion of the clinical occurrence of the organism, bacteriological characteristics, and antibiotic sensitivities is presented.	1977	914996
studies on the lipase of chromobacterium viscosum. v. physical and chemical properties of the lipases.		1977	922980
study of the nature of the associated microflora in the prolonged substrateless cultivation of beet plants.	the purpose of the present investigation was to study variations in the population density of microorganisms, their qualitative and quantitative composition during substrate-free cultivation of beats. the results obtained show that the microbial number in the nutrient solution increased by the time of intensive growth of beats and decreased by the time of harvesting. the number of root microorganisms was much greater than the total number of microorganisms in the nutrient solution. oligonitrophi ...	1977	17774
purification of lipase from chromobacterium viscosum by chromatography on palmitoyl cellulose.	palmitoyl cellulose was used to adsorb the extracellular lipase triacylglycerol acyl-hydrolase ec 3.1.1.3 of chromobacterium viscosum from crude enzyme solution, and the adsorbed enzyme was eluted with a suitable detergent, such as adekatol 45-s-8 or triton x-100. the enzyme was then purified by chromatography on a palmitoylated gauze column with an overall recovery of 71% and an increase in the specific activity of 11-fold from the supernatant fluid of bacterial cultures. further purification p ...	1977	19442
[biochemical tests and nutritional requirements of chromobacterium violaceum].		1977	279056
studies on the production of extracellular proteinases by a non-pigmented strain of chromobacterium lividum isolated from abattoir effluent.		1978	29882
[infections due to chromobacterium, serratia, erwinia and flavobacterium (the so-called chromobacterioses)].		1978	361381
stimulation of chromobacterium lipase activity and prevention of its adsorption to palmitoyl cellulose by hydrophobic binding of fatty acids.	fatty acids prevented adsorption of purified chromobacterium lipase [triacylglycerol acylhydrolase, ec 3.1.1.3] onto palmitoyl cellulose (pal-c) and also increased the activity of the purified lipase. these effects increased with increase in the concentration and chainlength (up to 16 carbon atoms) of the fatty acids, and long-chain unsaturated fatty acids, such as oleic acid, linoleic acid and erucic acid, were most effective. when the lipase was adsorbed (immobilized) on pal-c, its activity wa ...	1978	659403
microbiology problem: chromobacterium violaceum infection.		1979	386795
[combination of 5-fluorocytosine and amphotericin b in the treatment of chromomycosis].	the authors discuss the problem of resistance of chromomycosis to 5-fluorocytosine (5-fc), observed in a previous study in 7 patients initially treated with the drug and subsequently treated with 5-fc associated with other medicaments. all the associations, including that with amphotericin b, failed to overcome resistance. experiments in mice and "in vitro" show a synergism between 5-fc and amphotericin b against "c. albicans", "c. neoformans" and "fonsecaeae pedrosoi" already confirmed in huma ...	1979	398931
fatal infection by chromobacterium violaceum--clinical and bacteriological study.		1979	457214
osteomyelitis associated with chromobacterium violaceum sepsis. a case report.		1979	479248
phenylalanine hydroxylase from chromobacterium violaceum. purification and characterization.	phenylalanine hydroxylase was purified approximately 3000-fold to apparent homogeneity with a 13% yield and crystalized from l-phenylalanine-induced cells of chromobacterium violaceum. the enzyme was shown to be composed of a single polypeptide chain with an estimated molecular weight of approximately 32,000. some of the physical properties of the enzyme include: a stokes radius of 26.0 a, a sedimentation coefficient of 2.71 s, a diffusion coefficient of 8.20 x 10(-7) cm2/s, a frictional ratio o ...	1979	33985
chromobacterium violaceum infections. report of a fatal case.	a case of systemic chromobacterium violaceum infection, believed to be the first reported in a papua new guinean, is described. autopsy disclosed multiple liver abscesses and the diagnosis was made by recovery of the organism from one of the abscesses. aspects of management of this uncommon infection are discussed.	1979	299336
septicemia and death caused by chromobacterium violaceum.		1980	7403928
chromobacteriosis rare, but early diagnosis vital.		1981	7463651
the laboratory diagnosis of opportunistic infections caused by uncommon bacteria in papua new guinea.	flavobacterium meningosepticum, chromobacterium violaceum and listeria monocytogenes are opportunistic pathogens of man and are occasional but important causes of infections in neonates, infants and adults in papua new guinea. mortality is high. flavobacterium meningosepticum should be suspected when a weak indole producing oxidase positive gram-negative bacillus producing a discrete yellow non-diffusing pigment at room temperature and showing a resistant antibiotic sensitivity pattern is isolat ...	1981	6951339
monobactams: isolation and structure determination.		1981	7199043
influence of methylheptenone and related phytoplankton norcarotenoids on heterotrophic aquatic bacteria.	certain norcarotenoids, which have recently been found as excretion products of freshwater cyanobacteria and algae, are potent inhibitors of different metabolic functions in heterotrophic bacteria. 6-methylhept-5-en-2-one showed the strongest effects and acted as a noncompetitive inhibitor of both glucose uptake and respiration by aquatic isolates of chromobacterium lividum and arthrobacter sp. inhibition of the heterotrophic potential of glucose uptake by 6-methylhept-5-en-2-one was characteriz ...	1981	7214229
attempts at induction of phenylalanine hydroxylase in thermophilic bacteria and induction of thermophily in mesophiles.	some moderate and extreme thermophilic bacteria grew well on media other than the recommended basic media. attempts to induce phenylalanine hydroxylase (phe h) in the various thermophiles as well as mild thermophily in the mesophiles pseudomonas sp atcc 11299a and chromobacterium violaceum atcc 12540 were unsuccessful. evidence is presented indicating that the enzyme in the latter two organisms may be membrane bound. the level of phe h activity induced was not always consistent with the level of ...	1981	7244367
chromobacterium violaceum presenting as a surgical emergency.		1981	7280766
fulminating chromobacterial septicaemia presenting as respiratory distress syndrome.		1981	7314030
[lipases of gram-negative bacteria: a review].	the review surveys current concepts concerning properties of lipases from gram-negative bacteria. it discusses the effects of cultivation conditions (composition, temperature and ph of the medium) of bacteria-producers on the biosynthesis of lipases.	1981	7017672
numerical taxonomy analysis of bacteria isolated from the completed 'most probable numbers' test for coliform bacilli.		1981	7024232
monobactams-antibiotics from the marshes.		1981	6116095
chromobacteriosis--florida.		1981	6789054
microbiological quality of selected recreational waters.		1981	6797820
the effect of some varying lipid a structures on the inhibition of fibrillogenesis in basement membrane collagen.	acid soluble basement membrane collagen (abmc) was prepared by extraction of the anterior lens capsules from bovine calf eyes in 0.5 m acetic acid in the presence of the protease inhibitors leupeptin and pepstatin. thermal aggregates formed from soluble basement membrane collagen were facilitated by heating (28 degree c) the collagen solutions in 0.15 m phosphate buffer. the effects of endotoxins derived from salmonella minnesota r595, chromobacterium violaceum an rhodopseudomonas viridis on the ...	1982	6801242
biosynthesis of monobactam compounds: origin of the carbon atoms in the beta-lactam ring.	the biosynthesis of monobactams by strains of chromobacterium violaceum, acetobacter sp., and agrobacterium radiobacter was studied. monobactams were produced during logarithmic growth by c. violaceum and acetobacter sp. and during late log growth on glycerol and in stationary phase by a. radiobacter. the addition of various amino acids failed to significantly stimulate monobactam production in any of the producing organisms. several 14c-amino acids and pyruvate were incorporated in vivo into mo ...	1982	6805424
dna:rrna hybridization studies of chromobacterium fluviatile.	hybrids were prepared between 14c-labelled rrna from each of nine species of gram-negative bacteria and the dna of chromobacterium fluviatile. two parameters were determined for each hybrid - the t m(e), which is the temperature at which 50% of the hybrid was denatured, and the percentage of rrna bound under defined stringent conditions. the former gives a measure of the stability of the duplex once formed and the latter probability reflects the amount of the genome involved in the coding of rrn ...	1982	6181184
sq 26,180, a novel monobactam. ii isolation, structure determination and synthesis.		1982	7076565
chronic granulomatous disease of childhood and chromobacterium violaceum infections in the southeastern united states.	patients with chronic granulomatous disease are predisposed to infections by catalase-positive organisms in the environment. chromobacterium violaceum is a catalase-positive bacterium whose saprophytic source in this country is the subtropical soil and water of the southeastern united states. two patients with chronic granulomatous disease, followed at the national institutes of health in maryland, acquired c. violaceum infections in florida. all 10 cases previously reported were acquired in flo ...	1982	7092006
complete hematologic and hepatic recovery in a patient with chloramphenicol hepatitis-pancytopenia syndrome.		1982	7143157
isolation of chromobacterium spp. from foods, soil, and water.	chromobacterium violaceum, a soil and water inhabitant, has been implicated in human disease with a high mortality rate, particularly in the southeastern united states. the psychrotrophic chromobacterium lividum has been isolated from foods, water, and soil, but is not considered pathogenic. to determine the distribution of chromobacterium spp. in soil, water, and foods in the gainesville area, we evaluated bennett, ryalls and moss, and aeromonas membrane agars for their ability to recover these ...	1982	7159087
distribution of beta-lactam and beta-lactone producing bacteria in nature.	over one million bacteria were isolated from a large variety of soil, plant and water samples collected from different environments and examined in an extremely sensitive and highly specific screen for beta-lactam production. a group of seven related monocyclic beta-lactams (monobactams) were isolated from strains representing four genera-agrobacterium, chromobacterium, gluconobacter and pseudomonas. monobactam-producing strains of agrobacterium and pseudomonas were isolated only rarely. produci ...	1982	7174535
the similarities between pseudomonas paucimobilis and allied bacteria derived from analysis of deoxyribonucleic acids and electrophoretic protein patterns.	the chromosomal dna was isolated and purified from 17 strains of pseudomonas paucimobilis, and from the type or reference strains of flavobacterium capsulatum, f. devorans, f. multivorum, 'chromobacterium lividum', xanthomonas campestris and seven species of pseudomonas. the dna base compositions (mol% g + c) of p. paucimobilis strains were between 62.2 and 68.6%, and typical strains had a mean value of 65.3 +/- 0.4 mol%, determined from thermal denaturation temperature. dna-dna molecular hybrid ...	1982	7183747
chromobacterium violaceum septicemia in new jersey.		1982	6953253
sq 26,180, a novel monobactam. i taxonomy, fermentation and biological properties.	strains of chromobacterium violaceum, isolated from various environments, were found to produce a novel monocyclic beta-lactam antibiotic. the antibiotic, sq 26,180 was weakly active against gram-positive and gram-negative bacteria with the exception of mutants hypersensitive to beta-lactam antibiotics. the compound was highly stable to beta-lactamases and acted as an inhibitor of the p99 enzyme from enterobacter cloacae. sq 26,180 showed affinity for penicillin-binding proteins 1a, 4 and 5/6 of ...	1982	6978877
diversity dynamics of marine bacteria studied by immunofluorescent staining on membrane filters.	of 34 strains of marine bacteria isolated on a general seawater medium, 5 were selected for detailed studies of their population dynamics in the plankton. the isolates were characterized as aeromonas sp., chromobacterium cf. lividum, vibrio sp., and two pseudomonas spp. specific antibodies were produced by immunization of rabbits, and bacterial cells were stained on black uni-pore membrane filters by an indirect immunofluorescent staining procedure. the method proved to be very specific and prac ...	1982	16345920
chromobacterium violaceum septicaemia in a dog.		1983	6314965
chromobacterium violaceum infectious and chronic granulomatous disease.		1983	6824267
a new antibiotic, 3,6-dihydroxyindoxazene.		1983	6853374
effect of bestatin analogues and other compounds on enkephalin hydrolysis by an aminopeptidase from the mesophiles pseudomonas sp atcc 11299a and chromobacterium violaceum atcc 12540.	in our studies on newly synthesized compounds for their potential analgesic effect, we decided for purposes of convenience and economy to investigate non-mammalian sources for the presence of enkephalin degrading enzymes. an aminopeptidase that catalyzes the hydrolysis of the tyrosylglycyl bond of leucine- and methionine enkephalin was purified from the mesophiles pseudomonas sp atcc 11299a (ps) and chromobacterium violaceum atcc 12540 (cv). each preparation also hydrolyzed to varying extents ne ...	1983	6856949
sulfur metabolism in the biosynthesis of monobactams.	we studied the biosynthesis of monobactams with respect to sulfur metabolism in chromobacterium violaceum, acetobacter sp., and agrobacterium radiobacter. all three organisms used inorganic sulfur for monobactam production. when sulfur-containing amino acids were assayed as a source of sulfur for monobactam production, c. violaceum used cystine but not cysteine or methionine, acetobacter sp. used all three compounds, and a. radiobacter used none. 35s from cysteine, methionine, and sodium sulfate ...	1983	6859838
a probable case of chromobacterium violaceum infection in australia.	a case of a north-queensland aboriginal child, who died of a septicaemic illness probably caused by chromobacterium violaceum, is reported. this organism is a gram-negative bacillus found in soil and water. human and animal infections are very rare, but have been reported from various tropical and subtropical areas of the world. most of these have been fatal, although survival after recognition, appropriate antibiotic therapy, and surgical intervention has also been reported.	1983	6865828
a new antibiotic y-t0678h produced by a chromobacterium species.		1983	6615590
microbial growth associated with granular activated carbon in a pilot water treatment facility.	the microbial dynamics associated with granular activated carbon (gac) in a pilot water treatment plant were investigated over a period of 16 months. microbial populations were monitored in the influent and effluent waters and on the gac particles by means of total plate counts and atp assays. microbial populations between the influent and effluent waters of the gac columns generally increased, indicating microbial growth. the dominant genera of microorganisms isolated from interstitial waters a ...	1983	6625567
arphamenines a and b, new inhibitors of aminopeptidase b, produced by bacteria.		1983	6654760
isolation and identification of ropy bacteria in raw milk.	approximately 4.2% of 4,000 maryland-virginia raw milk tanker samples developed ropiness when incubated at 10 degrees c. of the 56 bacterial isolates 30 were identified by species. klebsiella oxytoca and pseudomonas aeruginosa were isolated most frequently. other ropy isolates were identified as pseudomonas spp., chromobacterium, flavobacterium multivorum, presumptive yersinia pestis, enterobacter agglomerans, klebsiella pneumoniae, and pasteurella-actinobacter spp. six of the klebsiella oxytoca ...	1983	6685140
[qualitative and quantitative determination of bacterial populations in aquatic habitats. 5. comparison of 2 rapid sand filters].	the bacterial examinations of raw water and the effluent of two rapid sand filters showed differences with respect to the colony count, the identified species and the activity of the isolated bacteria. the raw waters of the treatment plants si and me are originated from an oligotrophic artificial lake and from ground-water respectively. the two raw waters contained only low numbers of bacteria, whereas the effluents of the filters showed a considerable increase of the bacterial counts. the resul ...	1983	6367299
nature and location of amide-bound (r)-3-acyloxyacyl groups in lipid a of lipopolysaccharides from various gram-negative bacteria.	it has previously been demonstrated [eur. j. biochem. 124, 191-198 (1982) and 137, 15-22 (1983)] that the lipid a component of salmonella and proteus lipopolysaccharides contains amide-linked (r)-3-acyloxyacyl residues. in the present study lipid a of other gram-negative bacteria was analysed for the presence of amide-bound 3-acyloxyacyl residues. it was found that such residues are constituents of all lipid a tested (agrobacterium tumefaciens, chromobacterium violaceum, pseudomonas aeruginosa, ...	1984	6437812
specificity and mechanism of ferrioxamine-mediated iron transport in streptomyces pilosus.	although the ferrioxamines are an important and well-characterized class of siderophores produced by several species of nocardia, streptomyces, micromonospora, arthrobacter, chromobacterium, and pseudomonas, no studies of the mechanism of ferrioxamine-mediated iron uptake have been performed for an organism which produces the siderophore. this is the first report of metal transport in streptomyces pilosus mediated by the native ferrioxamines b, d1, d2, and e. 55fe accumulation in these ferrioxam ...	1984	6480557
prosthetic valve endocarditis caused by group ve-1 bacteria (chromobacterium typhiflavum).	a case of fatal prosthetic valve endocarditis was caused by group ve-1 bacteria. bacteriological characteristics and antibiotic susceptibilities are presented, as well as a brief discussion of the isolates of the ve-1 organism in ontario.	1984	6511882
chromobacterium violaceum as a cause of periorbital cellulitis.		1984	6514595
hepatic drug-metabolizing enzyme system and endotoxin tolerance: structural requirement of lps in induction of an early tolerance.	the alteration of hepatic drug-metabolizing enzyme activities in mice given salmonella endotoxin by single or multiple intraperitoneal injections was investigated. an essentially the same biphasic, early and late phase, endotoxin tolerance was observed in the animals receiving a single injection of endotoxin or repetitive daily injections. the results of reciprocal cross tolerance tests using lipopolysaccharide and free lipid a preparations derived from salmonella minnesota, salmonella typhimuri ...	1984	6521669
[growth rate of antibiotic-producing gram-negative bacteria on liquid nutrient media].	glycerol-yeast medium no. 3 may be used as a seed medium in screening of antibiotic-producing strains among acetobacter, gluconobacter, chromobacterium, agrobacterium, and other genera. the medium is transparent. it provides visual instrumental control of the growth rate of the seed material and estimation of biomass augmentation. the period of the exponential phase growth of the strains tested on medium no. 3 was 2-8 hours. when no growth on medium no. 3 is observed media nos. 1 and 2 can be us ...	1984	6524878
human infection due to chromobacterium violaceum: a report from ilorin, nigeria.		1984	6535708
heterotrophic nitrification among denitrifiers.	twelve denitrifying bacteria representing six genera were tested for an ability to nitrify pyruvic oxime heterotrophically. six of these bacteria exhibited appreciable nitrification activity, yielding as much as 5.8 mm nitrite and little or no nitrate when grown in a mineral salts medium containing 7 mm pyruvic oxime and 0.05% yeast extract. of the six active bacteria, four (pseudomonas denitrificans, pseudomonas aeruginosa, and two strains of pseudomonas fluorescens) could grow on yeast extract ...	1984	6721486
chromobacterium violaceum infection of the eye. a report of two cases.	we report what we believe to be the first two known cases of chromobacterium violaceum infection of the eye. one patient had conjunctivitis as the initial manifestation of an ultimately fatal infection. in the second patient, who suffered from chronic granulomatous disease of childhood, orbital cellulitis developed as part of a disseminated c violaceum infection from which he subsequently recovered. infections caused by c violaceum may involve the eye and orbit. these infections are frequently f ...	1984	6721759
the effects of the insecticide acephate on the growth and nutrient uptake of an aquatic bacterium.	the effects of high (1000 ppm) and low (1 ppm) concentrations of acephate on the rate of growth and nutrient uptake by an aquatic bacterium (identified as chromobacterium lividum ) were investigated. this insecticide increased doubling time, decreased maximum cellular yield, and reduced cell size when c. lividum was grown in the presence of high acephate concentrations. total [14c]glucose and 14c-labeled amino acids uptake rates were reduced by the high acephate concentration. the high acephate ...	1984	6722663
biosynthetic studies of arphamenines a and b.	the biosynthetic pathways of arphamenines a and b were studied. arphamenine a was derived from acetic acid, l-arginine and l-phenylalanine, and arphamenine b from acetic acid, l-arginine and l-tyrosine.	1984	6735925
[effect of solid substrates of microbial colony development].	bacteria have been found to grow differently at contact sites to both organic and anorganic solids as on pure agar media. of 16 ad hoc selected bacterial strains, the following species (arranged in decreasing order) responded with stronger colony formation: bacillus subtilis, b. cereus, streptococcus sanguis, s. saprophyticus, s. faecalis, escherichia coli, micrococcus flavus, actinomyces viscosus, veillonella sp., enterobacter liquefaciens, yersinia enterocolitica. lactobacillus casei behaved n ...	1984	6326398
[bacteria and dietary fiber].	the presence of cellulose particles, so in-vitro studies have shown, influences the growth rate and metabolic activity of the bacteria escherichia coli, bifidobacterium adolescentis and chromobacterium violaceum. after the homogenization in waring blendor, the growth rate has been found to be highest in a medium containing 10% cellulose particles and to show a decreasing tendency in media containing 5%, 1% and 0% cellulose. the biochemical intensity, judged by glucose consumption and intensity o ...	1984	6326399
recurrent infection with chromobacterium violaceum: first case report from south america.	a case of chromobacteriosis in a young brazilian with toxaemia and multiple skin abscesses is described. the infection responded to treatment with chloramphenicol and cotrimoxazole but recurred 18 months later following insect bites received while fishing in a river. chromobacterium violaceum was subsequently isolated from the river water. this is the first case of this kind to be reported from south america.	1984	6334119
novel potentiators of beta-lactam antibiotics. isolation of sq28,504 and sq28,546 from chromobacterium violaceum.	two novel compounds, sq28,504 and sq28,546 are produced by chromobacterium violaceum. these compounds enhance the antibacterial activity of beta-lactam antibiotics against gram-negative organisms. both sq28,504 and sq28,546 induce morphological changes in the presence of beta-lactam antibiotics. only sq28,546 has weak antimicrobial activity against several gram-negative organisms.	1985	3839229
quantitative studies of heat-stable proteinase from pseudomonas fluorescens p1 by the enzyme-linked immunosorbent assay.	pseudomonas fluorescens p1 is a psychrotrophic bacterium isolated from milk. proteinase p1, the main extracellular heat-stable proteinase fraction of p. fluorescens p1, has been purified to homogeneity. a procedure with a sandwich enzyme-linked immunosorbent assay, using microplates and alkaline phosphatase conjugate was shown to detect 0.25 ng of proteinase p1 in 1 ml of reconstituted skim milk or defatted cream. the method offers the combination of sensitivity and specificity for the detection ...	1985	3920965
chromobacterium violaceum adenitis acquired in the northern united states as a complication of chronic granulomatous disease.		1985	4080593
unusual mucocutaneous infections in immunosuppressed patients with leukemia--expansion of an earlier study.		1986	3513146
microbial cyanide metabolism.		1986	3532718
cell-free biosynthesis of arphamenine a.	arphamenine a was synthesized in a cell-free system obtained from the arphamenine-producing strain, chromobacterium violaceum bmg361-cf4. l-[14c]-phenylalanine was converted to beta-phenylpyruvic acid by phenylalanine amino-transferase obtained from the 10,000 x g supernatant (s10 fraction). [14c]-benzylmalic acid was synthesized from beta-phenylpyruvic acid with [14c]-acetyl-coa in the s10 fraction. [14c]-benzylsuccinic acid was formed from beta-phenylpyruvic acid with [14c]-acetyl-coa and atp ...	1986	3707594
first case report from argentina of fatal septicemia caused by chromobacterium violaceum.	a case of chromobacterium violaceum fatal septicemia is reported. the microorganism was isolated from six blood cultures and two suppurated skin lesions. it is the first such case described in argentina, and it reinforces the need for prolonged treatment and careful clinical evaluation to ensure complete remission of human infections caused by this bacterium.	1986	3711283
nonfatal chromobacterial sepsis.	we have described a case of multiple liver abscesses caused by chromobacterium violaceum. this organism should be considered in any case of unexplained liver abscess occurring in the southeastern united states. successful treatment of liver abscesses caused by this organism may be accomplished with appropriate antibiotic therapy.	1986	3750002
isolation and characterization of aerobic heterotrophic bacteria from natural spring waters in the lanjaron area (spain).	aerobic, heterotrophic bacteria were isolated from nine natural mineral water springs in the lanjaron area of spain over the period july 1980 to may 1981. the mineral waters contained few bacteria (mean counts 26-5275 cfu per 100 ml) and the bacterial flora of all nine springs was very similar. most of the isolates were gram-negative rods (90%), and among these pseudomonas spp. and members of the flavobacterium-cytophaga-flexibacter group were numerically dominant. aeromonas-vibrio and enterobac ...	1986	3781942
[fatal septicemia due to chromobacterium violaceum].	a 2-year old boy was suffered from an infection caused by chromobacterium violaceum. this is the first case of c. violaceum infection reported in taiwan. the boy was treated with chloramphenicol and gentamicin but died after one month of illness due to the incooperation of patient's family. the causative agent was isolated from patient's blood and scar. complete bacteriologic characteristics and antimicrobial susceptibility pattern of this isolate are reported.	1986	3816371
[chromobacterium violaceum infection. presentation of a case with fatal outcome].		1986	3299554
the mechanism of action of phenylalanine hydroxylase.		1986	3014980
phenylalanine hydroxylase from chromobacterium violaceum is a copper-containing monooxygenase. kinetics of the reductive activation of the enzyme.	pterin-dependent phenylalanine hydroxylase from chromobacterium violaceum contains a stoichiometric amount of copper (cu2+, 1 mol/mol of enzyme). electron paramagnetic resonance spectroscopy of the enzyme indicates that it is a type ii copper-containing protein. the oxidized enzyme must be reduced by a single electron to be catalytically active. dithiothreitol was found to be an effective reducing agent for the enzyme. electron paramagnetic resonance data and kinetic results indicate the formati ...	1986	3024714
variability of the influence of physicochemical factors affecting bacterial adhesion to polystyrene substrata.	the role of electrostatic and hydrophobic interactions and solid and liquid surface tensions in the adhesion of four bacterial species (pseudomonas fluorescens, enterobacter cloacae, chromobacterium sp., and flexibacter sp.) to hydrophobic polystyrene petri dishes and to more hydrophilic polystyrene tissue culture dishes was investigated. the effect of electrostatic interactions was investigated by determining the effects of different electrolyte solutions on attachment to and of different elect ...	1986	16347145
denitrification by chromobacterium violaceum.	one host (rana catesbiana)-associated and two free-living mesophilic strains of bacteria with violet pigmentation and biochemical characteristics of chromobacterium violaceum were isolated from freshwater habitats. cells of each freshly isolated strain and of strain atcc 12472 (the neotype strain) grew anaerobically with glucose as the sole carbon and energy source. the major fermentation products of cells grown in trypticase soy broth (bbl microbiology systems, cockeysville, md.) supplemented w ...	1986	16347164
adduct formation between the cupric site of phenylalanine hydroxylase from chromobacterium violaceum and 6,7-dimethyltetrahydropterin.	the interaction of pterin-dependent phenylalanine hydroxylase from chromobacterium violaceum with the cofactor analogue 5-deaza-6-methyltetrahydropterin and the cofactor 6,7-dimethyltetrahydropterin (dmph4) has been investigated by multifrequency electron spin resonance (esr) spectroscopy. 5-deaza-6-methyltetrahydropterin, which lacks the n-5 nitrogen present in the pyrazine ring of dmph4, binds tightly to the cupric form of the enzyme; however, no changes are observed in the esr parameters of t ...	1987	2822093
natural microbiologic hazards of the aquatic environment.		1987	3311341
[role of halophilic and halotrophic bacteria in the chronic problems of long-term dialysis].		1987	3319061
phenylalanine 4-monooxygenase from chromobacterium violaceum.		1987	2885717
[use of chromobacterin to control the larvae of blood-sucking mosquitoes].		1987	3448483
chromobacterium violaceum meningitis--a case report.		1987	3449453
mercury-resistance and mercuric reductase activity in chromobacterium, erwinia, and bacillus species.		1987	3580613
chromobacterium violaceum phenylalanine 4-monooxygenase.		1987	3600378
[mobilization of granulocyte-macrophage colony-forming cells (cfu-gm) into the peripheral blood of the mouse by ch. violaceum endotoxin].		1987	3603064
bacteremia caused by cdc group ve-1 in previously healthy patient with granulomatous hepatitis.	this is a case report of cdc group ve-1 bacteremia in an otherwise healthy patient with granulomatous hepatitis.	1987	3667927
glucose-6-phosphate dehydrogenase deficiency, neutrophil dysfunction and chromobacterium violaceum sepsis.		1987	3681551
aerocavin, a new antibiotic produced by chromobacterium violaceum.	a new antibiotic, aerocavin, has been isolated from fermentation broths of a non-pigmented strain of chromobacterium violaceum. the structure 1 was deduced from its spectroscopic properties and x-ray diffraction analysis. aerocavin exhibits activity in vitro against gram-positive and gram-negative bacteria.	1988	3372350
aerocyanidin, a new antibiotic produced by chromobacterium violaceum.	aerocyanidin, a new antibiotic containing an isonitrile group, has been isolated from fermentations of chromobacterium violaceum atcc 53434. structure 1 was assigned on the bais of spectroscopic characterization of the antibiotic and of a degradation product that results from treatment with base. the antibiotic is primarily active against gram-positive bacteria.	1988	3372351
a comparative study of virulent and avirulent strains of chromobacterium violaceum.	a clinical isolate and a soil isolate of chromobacterium violaceum were compared to determine differences in virulence-related characteristics. purified lipopolysaccharide (endotoxin) from the virulent, clinical strain was more reactive than that from the avirulent soil strain as determined by the limulus amebocyte lysate assay. there were no differences in hemolysin or cyanide production between the two strains. the virulent strain was more resistant to phagocytosis and intracellular killing by ...	1988	2843271
partial purification and characterization of adp sulfurylase from the purple sulfur bacterium thiocapsa roseopersicina.	high activities of adp and atp sulfurylase were found in the soluble protein fraction of thiocapsa roseopersicina strain 6311 (dsm 219). adp sulfurylase was partially purified and characterized. it was a very labile soluble enzyme with a molecular weight of 250,000. the optimum ph was 7.5 and the optimal temperature 35 degrees c. under test conditions the apparent km values were determined to be 0.33 mm for adenylylsulfate and 13 mm for phosphate.	1988	2855979