Publications

acetification of rice wine by acetobacter aceti using loofa sponge in a low-cost reciprocating shaker.	to maximize acetification rate (eta) by adsorption of acetic acid bacteria (aab) on loofa sponge matrices (lsm).	2014	25176134
mitochondrially-targeted bacterial phosphatidylethanolamine methyltransferase sustained phosphatidylcholine synthesis of a saccharomyces cerevisiae δpem1 δpem2 double mutant without exogenous choline supply.	in eukaryotic cells, phospholipids are synthesized exclusively in the defined organelles specific for each phospholipid species. to explain the reason for this compartmental specificity in the case of phosphatidylcholine (pc) synthesis, we constructed and characterized a saccharomyces cerevisiae strain that lacked endogenous phosphatidylethanolamine (pe) methyltransferases but had a recombinant pe methyltransferase from acetobacter aceti, which was fused with a mitochondrial targeting signal fro ...	2014	24832487
acetobacter sicerae sp. nov., isolated from cider and kefir, and identification of species of the genus acetobacter by dnak, groel and rpob sequence analysis.	five acetic acid bacteria isolates, awk9_3, awk9_4 ( = lmg 27543), awk9_5 ( = lmg 28092), awk9_6 and awk9_9, obtained during a study of micro-organisms present in traditionally produced kefir, were grouped on the basis of their maldi-tof ms profile with lmg 1530 and lmg 1531(t), two strains currently classified as members of the genus acetobacter. phylogenetic analysis based on nearly complete 16s rrna gene sequences as well as on concatenated partial sequences of the housekeeping genes dnak, gr ...	2014	24763601
utilization of elongation factor tu gene (tuf) sequencing and species-specific pcr (ss-pcr) for the molecular identification of acetobacter species complex.	the aim of this study was to use tuf gene as a molecular target for species discrimination in the acetobacter genus, as well as to develop species-specific pcr method for direct species identification of acetobacter aceti. the results showed that most acetobacter species could be clearly distinguished, and the average sequence similarity for the tuf gene (89.5%) among type strains was significantly lower than that of the 16s rrna gene sequence (98.0%). a pair of species-specific primers were des ...	2014	23969032
sbng, a citrate synthase in staphylococcus aureus: a new fold on an old enzyme.	in response to iron deprivation, staphylococcus aureus produces staphyloferrin b, a citrate-containing siderophore that delivers iron back to the cell. this bacterium also possesses a second citrate synthase, sbng, that is necessary for supplying citrate to the staphyloferrin b biosynthetic pathway. we present the structure of sbng bound to the inhibitor calcium and an active site variant in complex with oxaloacetate. the overall fold of sbng is structurally distinct from tca cycle citrate synth ...	2014	25336653
structure and functional characterization of pyruvate decarboxylase from gluconacetobacter diazotrophicus.	bacterial pyruvate decarboxylases (pdc) are rare. their role in ethanol production and in bacterially mediated ethanologenic processes has, however, ensured a continued and growing interest. pdcs from zymomonas mobilis (zmpdc), zymobacter palmae (zppdc) and sarcina ventriculi (svpdc) have been characterized and zmpdc has been produced successfully in a range of heterologous hosts. pdcs from the acetobacteraceae and their role in metabolism have not been characterized to the same extent. examples ...	2014	25369873
metagenome-wide association of microbial determinants of host phenotype in drosophila melanogaster.	animal-associated bacteria (microbiota) affect host behaviors and physiological traits. to identify bacterial genetic determinants of microbiota-responsive host traits, we employed a metagenome-wide association (mgwa) approach in two steps. first, we measured two microbiota-responsive host traits, development time and triglyceride (tag) content, in drosophila melanogaster flies monoassociated with each of 41 bacterial strains. the effects of monoassociation on host traits were not confined to pa ...	2014	25271286
effects of aromatic compounds on the production of bacterial nanocellulose by gluconacetobacter xylinus.	bacterial cellulose (bc) is a polymeric nanostructured fibrillar network produced by certain microorganisms, principally gluconacetobacter xylinus. bc has a great potential of application in many fields. lignocellulosic biomass has been investigated as a cost-effective feedstock for bc production through pretreatment and hydrolysis. it is well known that detoxification of lignocellulosic hydrolysates may be required to achieve efficient production of bc. recent results suggest that phenolic comp ...	2014	24884902
molecular detection of bacteria in plant tissues, using universal 16s ribosomal dna degenerated primers.	highly specific, sensitive and rapid tests are required for the detection and identification of covert bacterial contaminations in plant tissue cultures. current methods available for this purpose are tedious, time consuming, highly error prone, expensive, require advanced technical expertise and are sometimes ineffective. we report here the development of a sensitive polymerase chain reaction (pcr) based method for the rapid detection and identification of bacteria occurring in plant tissue cul ...	2014	26019546
evolution of mitochondria reconstructed from the energy metabolism of living bacteria.	the ancestors of mitochondria, or proto-mitochondria, played a crucial role in the evolution of eukaryotic cells and derived from symbiotic α-proteobacteria which merged with other microorganisms - the basis of the widely accepted endosymbiotic theory. however, the identity and relatives of proto-mitochondria remain elusive. here we show that methylotrophic α-proteobacteria could be the closest living models for mitochondrial ancestors. we reached this conclusion after reconstructing the possibl ...	2014	24804722
antioxidant and antibacterial activity of the beverage obtained by fermentation of sweetened lemon balm (melissa officinalis l.) tea with symbiotic consortium  of bacteria and yeasts.	kombucha is a fermented tea beverage which is traditionally prepared by fermenting sweetened black or green tea (camellia sinensis l.) with symbiotic consortium of bacteria and yeasts (scoby). in this study, lemon balm (melissa officinalis l.) was used as the only nitrogen source for kombucha fermentation. during the seven-day fermentation process, ph value, titratable acidity (ta), total phenolic content, phenolic compounds, and antioxidant activity against hydroxyl (˙oh) and 1,1-diphenyl-2-pic ...	2014	27904315
attachment of asaia bogorensis originating in fruit-flavored water to packaging materials.	the objective of this study was to investigate the adhesion of isolated spoilage bacteria to packaging materials used in the food industry. microorganisms were isolated from commercial fruit-flavored mineral water in plastic bottles with flocks as a visual defect. the gram-negative rods were identified using the molecular method through the amplification of a partial region of the 16s rrna gene. based on the sequence identity (99.6%) between the spoilage organism and a reference strain deposited ...	2014	25295262
the key to acetate: metabolic fluxes of acetic acid bacteria under cocoa pulp fermentation-simulating conditions.	acetic acid bacteria (aab) play an important role during cocoa fermentation, as their main product, acetate, is a major driver for the development of the desired cocoa flavors. here, we investigated the specialized metabolism of these bacteria under cocoa pulp fermentation-simulating conditions. a carefully designed combination of parallel 13c isotope labeling experiments allowed the elucidation of intracellular fluxes in the complex environment of cocoa pulp, when lactate and ethanol were inclu ...	2014	24837393
draft genome sequence of acetobacter aceti strain 1023, a vinegar factory isolate.	the genome sequence of acetobacter aceti 1023, an acetic acid bacterium adapted to traditional vinegar fermentation, comprises 3.0 mb (chromosome plus plasmids). a. aceti 1023 is closely related to the cocoa fermenter acetobacter pasteurianus 386b but possesses many additional insertion sequence elements.	2014	24903876
enzyme structure captures four cysteines aligned for disulfide relay.	thioredoxin superfamily proteins introduce disulfide bonds into substrates, catalyze the removal of disulfides, and operate in electron relays. these functions rely on one or more dithiol/disulfide exchange reactions. the flavoenzyme quiescin sulfhydryl oxidase (qsox), a catalyst of disulfide bond formation with an interdomain electron transfer step in its catalytic cycle, provides a unique opportunity for exploring the structural environment of enzymatic dithiol/disulfide exchange. wild-type ra ...	2014	24888638
acetic acid bacteria genomes reveal functional traits for adaptation to life in insect guts.	acetic acid bacteria (aab) live in sugar rich environments, including food matrices, plant tissues, and the gut of sugar-feeding insects. by comparing the newly sequenced genomes of asaia platycodi and saccharibacter sp., symbionts of anopheles stephensi and apis mellifera, respectively, with those of 14 other aab, we provide a genomic view of the evolutionary pattern of this bacterial group and clues on traits that explain the success of aab as insect symbionts. a specific pre-adaptive trait, c ...	2014	24682158
structural snapshots along the reaction pathway of yersinia pestis ripa, a putative butyryl-coa transferase.	yersinia pestis, the causative agent of bubonic plague, is able to survive in both extracellular and intracellular environments within the human host, although its intracellular survival within macrophages is poorly understood. a novel y. pestis three-gene rip (required for intracellular proliferation) operon, and in particular ripa, has been shown to be essential for survival and replication in interferon γ-induced macrophages. ripa was previously characterized as a putative butyryl-coa transfe ...	2014	24699651
draft genome sequence of gluconobacter thailandicus nbrc 3257.	gluconobacter thailandicus strain nbrc 3257, isolated from downy cherry (prunus tomentosa), is a strict aerobic rod-shaped gram-negative bacterium. here, we report the features of this organism, together with the draft genome sequence and annotation. the draft genome sequence is composed of 107 contigs for 3,446,046 bp with 56.17% g+c content and contains 3,360 protein-coding genes and 54 rna genes.	2014	25197448
effects of growth medium on matrix-assisted laser desorption-ionization time of flight mass spectra: a case study of acetic acid bacteria.	the effect of the growth medium used on the matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectra generated and its consequences for species and strain level differentiation of acetic acid bacteria (aab) were determined by using a set of 25 strains. the strains were grown on five different culture media that yielded a total of more than 600 mass spectra, including technical and biological replicates. the results demonstrate that the culture medium can have a profoun ...	2014	24362425
a distinct model of synergism between a processive endocellulase (tfcel9a) and an exocellulase (tfcel48a) from thermobifida fusca.	lignocellulosic biomass is digested in nature by the synergistic activities of enzymes with complementary properties, and understanding synergistic interactions will improve the efficiency of industrial biomass use for sustainable fuels and chemicals. cel9a and cel48a from a model bacterium, thermobifida fusca (tfcel9a and tfcel48a, respectively), are two cellulases with different properties and have previously been shown to synergize well with each other. tfcel9a is a processive endocellulase w ...	2014	24162578
molecular discrimination and identification of acetobacter genus based on the partial heat shock protein 60 gene (hsp60) sequences.	to identify the acetobacter species using phenotypic and genotypic (16s rdna sequence analysis) technique alone is inaccurate. the aim of this study was to use the hsp60 gene as a target for species discrimination in the genus acetobacter, as well as to develop species-specific polymerase chain reaction and mini-sequencing methods for species identification and differentiation.	2014	23681743
the rep20 replication initiator from the pag20 plasmid of acetobacter aceti.	in the previously isolated pag20 plasmid from the acetobacter aceti ccm3610 strain, the rep20 protein was characterized as a main replication initiator. the pag20 plasmid origin was localized in the vicinity of the rep20 gene and contained two 21-nucleotide-long iteron sequences, two 13-nucleotide-long direct repeats, and a dnaa-binding site. electrophoretic mobility shift assay and nonradioactive fragment analysis confirmed that the rep20 protein interacted with two direct repeats (5'-tccaaattt ...	2014	23839792
chronic administration of the probiotic kefir improves the endothelial function in spontaneously hypertensive rats.	the beverage obtained by fermentation of milk with kefir grains, a complex matrix containing acid bacteria and yeasts, has been shown to have beneficial effects in various diseases. however, its effects on hypertension and endothelial dysfunction are not yet clear. in this study, we evaluated the effects of kefir on endothelial cells and vascular responsiveness in spontaneously hypertensive rats (shr).	2015	26715471
bacterial ecology of fermented cucumber rising ph spoilage as determined by nonculture-based methods.	fermented cucumber spoilage (fcs) characterized by rising ph and the appearance of manure- and cheese-like aromas is a challenge of significant economical impact for the pickling industry. previous culture-based studies identified the yeasts pichia manshurica and issatchenkia occidentalis, 4 gram-positive bacteria, lactobacillus buchneri, lactobacillus parrafaraginis, clostridium sp., and propionibacterium and 1 gram-negative genus, pectinatus, as relevant in various stages of fcs given their ab ...	2015	26605993
bacterial ecology of fermented cucumber rising ph spoilage as determined by nonculture-based methods.	fermented cucumber spoilage (fcs) characterized by rising ph and the appearance of manure- and cheese-like aromas is a challenge of significant economical impact for the pickling industry. previous culture-based studies identified the yeasts pichia manshurica and issatchenkia occidentalis, 4 gram-positive bacteria, lactobacillus buchneri, lactobacillus parrafaraginis, clostridium sp., and propionibacterium and 1 gram-negative genus, pectinatus, as relevant in various stages of fcs given their ab ...	2015	26605993
milk kefir: composition, microbial cultures, biological activities, and related products.	in recent years, there has been a strong focus on beneficial foods with probiotic microorganisms and functional organic substances. in this context, there is an increasing interest in the commercial use of kefir, since it can be marketed as a natural beverage that has health promoting bacteria. there are numerous commercially available kefir based-products. kefir may act as a matrix in the effective delivery of probiotic microorganisms in different types of products. also, the presence of kefir' ...	2015	26579086
transcriptional modulation of squalene synthase genes in barley treated with pgpr.	phytosterol contents and food quality of plant produce is directly associated with transcription of gene squalene synthase (ss). in current study, barley plants were treated with different rhizobacterial strains under semi controlled (27 ± 3°c) greenhouse conditions in order to modulate expression of ss gene. plant samples were analyzed through semi-quantitative pcr to evaluate effect of rhizobacterial application on transcriptional status of ss. results revealed that among four ss genes (i.e., ...	2015	26388880
breeding strategy to generate robust yeast starter cultures for cocoa pulp fermentations.	cocoa pulp fermentation is a spontaneous process during which the natural microbiota present at cocoa farms is allowed to ferment the pulp surrounding cocoa beans. because such spontaneous fermentations are inconsistent and contribute to product variability, there is growing interest in a microbial starter culture that could be used to inoculate cocoa pulp fermentations. previous studies have revealed that many different fungi are recovered from different batches of spontaneous cocoa pulp fermen ...	2015	26150457
bacterial cellulose production from industrial waste and by-product streams.	the utilization of fermentation media derived from waste and by-product streams from biodiesel and confectionery industries could lead to highly efficient production of bacterial cellulose. batch fermentations with the bacterial strain komagataeibacter sucrofermentans dsm (deutsche sammlung von mikroorganismen) 15973 were initially carried out in synthetic media using commercial sugars and crude glycerol. the highest bacterial cellulose concentration was achieved when crude glycerol (3.2 g/l) an ...	2015	26140376
microbially influenced corrosion communities associated with fuel-grade ethanol environments.	microbially influenced corrosion (mic) is a costly problem that impacts hydrocarbon production and processing equipment, water distribution systems, ships, railcars, and other types of metallic infrastructure. in particular, mic is known to cause considerable damage to hydrocarbon fuel infrastructure including production, transportation, and storage systems, often times with catastrophic environmental contamination results. as the production and use of alternative fuels such as fuel-grade ethano ...	2015	26092755
glucosylceramide contained in koji mold-cultured cereal confers membrane and flavor modification and stress tolerance to saccharomyces cerevisiae during coculture fermentation.	in nature, different microorganisms create communities through their physiochemical and metabolic interactions. many fermenting microbes, such as yeasts, lactic acid bacteria, and acetic acid bacteria, secrete acidic substances and grow faster at acidic ph values. however, on the surface of cereals, the ph is neutral to alkaline. therefore, in order to grow on cereals, microbes must adapt to the alkaline environment at the initial stage of colonization; such adaptations are also crucial for indu ...	2015	25795678
genomic and metabolic analysis of fluoranthene degradation pathway in celeribacter indicus p73t.	celeribacter indicus p73(t), isolated from deep-sea sediment from the indian ocean, is capable of degrading a wide range of polycyclic aromatic hydrocarbons (pahs) and is the first fluoranthene-degrading bacterium within the family rhodobacteraceae. here, the complete genome sequence of strain p73(t) is presented and analyzed. besides a 4.5-mb circular chromosome, strain p73(t) carries five plasmids, and encodes 4827 predicted protein-coding sequences. one hundred and thirty-eight genes, includi ...	2015	25582347
a molecular description of cellulose biosynthesis.	cellulose is the most abundant biopolymer on earth, and certain organisms from bacteria to plants and animals synthesize cellulose as an extracellular polymer for various biological functions. humans have used cellulose for millennia as a material and an energy source, and the advent of a lignocellulosic fuel industry will elevate it to the primary carbon source for the burgeoning renewable energy sector. despite the biological and societal importance of cellulose, the molecular mechanism by whi ...	2015	26034894
a novel pyrroloquinoline quinone-dependent 2-keto-d-glucose dehydrogenase from pseudomonas aureofaciens.	a gene encoding an enzyme similar to a pyrroloquinoline quinone (pqq)-dependent sugar dehydrogenase from filamentous fungi, which belongs to new auxiliary activities (aa) family 12 in the cazy database, was cloned from pseudomonas aureofaciens. the deduced amino acid sequence of the cloned enzyme showed only low homology to previously characterized pqq-dependent enzymes, and multiple-sequence alignment analysis showed that the enzyme lacks one of the three conserved arginine residues that functi ...	2015	25645559
contribution to the prediction of the fold code: application to immunoglobulin and flavodoxin cases.	folding nucleus of globular proteins formation starts by the mutual interaction of a group of hydrophobic amino acids whose close contacts allow subsequent formation and stability of the 3d structure. these early steps can be predicted by simulation of the folding process through a monte carlo (mc) coarse grain model in a discrete space. we previously defined mirs (most interacting residues), as the set of residues presenting a large number of non-covalent neighbour interactions during such simu ...	2015	25915049
influence of calcium chloride in the high temperature acetification by strain acetobacter aceti wk for vinegar.	to improve the thermotolerant properties (ttp) of acetic acid bacteria (aab) cells for high temperature acetification.	2015	26268669
identification of microbial metabolites elevating vitamin contents in barley seeds.	the current investigation analyzes metabolites of acetobacter aceti to explore chemical compounds responsible for the induction of vitamins in barley seeds. a bioactivity guided assay of bacterial extracts and chromatographic analyses of barley produce revealed 13 chemical compounds, which were subjected to principal component analysis (pca). pca determined four chemical compounds (i.e., quinolinic acid, pyridoxic acid, p-aminobenzoate, and α-oxobutanoic acid) highly associated with increased qu ...	2015	26173019
acetobacter bacteria are found in zhenjiang vinegar grains.	zhenjiang vinegar, the grains of which contain a unique microbial flora, is one of the four famous traditional chinese vinegars. we investigated the components of zhenjiang vinegar grains. unique acetic acid bacteria were randomly isolated from zhenjiang vinegar grains, and the obtained strains were qualitatively analyzed to compare their capacities for acetate decomposition and acid production. acetic acid bacteria with a high acid-producing rate were identified by 16s rdna sequencing, and furt ...	2015	26125697
antimicrobial properties and mechanism of volatile isoamyl acetate, a main flavour component of japanese sake (ginjo-shu).	to evaluate the antimicrobial properties of the main ginjo-flavour components of sake, volatile isoamyl acetate and isoamyl alcohol.	2015	25626919
acetobacter musti sp. nov. isolated from bobal grape must.	an acetic acid bacterium (bo7t), obtained during a study of the microbial diversity of spontaneous fermentations of bobal grape must, was subjected to a polyphasic taxonomic study. phylogenetic analysis based on 16s rrna gene sequences allocated bo7t in the genus acetobacter, and revealed acetobacter aceti and acetobacter oeni to be nearest neighbours (99.57 % sequence similarity between bo7t and a. oeni cect 5830t, and 98.76 % between bo7t and a. aceti cect 298t). the cells are gram-negative mo ...	2015	26637821
application of the kombucha 'tea fungus' for the enhancement of antioxidant and starch hydrolase inhibitory properties of ten herbal teas.	ten herbal teas (acacia arabica, aegle marmelos flower, a. marmelos root bark, aerva lanata, asteracantha longifolia, cassia auriculata, hemidesmus indicus, hordeum vulgare, phyllanthus emblica, tinospora cordifolia) were fermented with the kombucha 'tea fungus'. the ph values of the fermented beverages ranged from 4.0 to 6.0 by day 7, while the titratable acidity ranged from 2.5 to 5.0g/ml (p<0.05). gallic acid had statistically significantly increased (p<0.05) in almost all the samples by day ...	2016	26471559
anticorrosive influence of acetobacter aceti biofilms on carbon steel.	microbiologically influenced corrosion (mic) of carbon steel infrastructure is an emerging environmental and cost issue for the ethanol fuel industry, yet its examination lacks rigorous quantification of microbiological parameters that could reveal effective intervention strategies. to quantitatively characterize the effect of cell concentration on mic of carbon steel, numbers of bacteria exposed to test coupons were systematically controlled to span four orders of magnitude throughout a seven-d ...	2016	28082824
surface-engineered saccharomyces cerevisiae displaying α-acetolactate decarboxylase from acetobacter aceti ssp xylinum.	to convert α-acetolactate into acetoin by an α-acetolactate decarboxylase (aldc) to prevent its conversion into diacetyl that gives beer an unfavourable buttery flavour.	2016	27623795
effect of composites based nickel foam anode in microbial fuel cell using acetobacter aceti and gluconobacter roseus as a biocatalysts.	this study explores the use of materials such as chitosan (chit), polyaniline (pani) and titanium carbide (tc) as anode materials for microbial fuel cells. nickel foam (nf) was used as the base anode substrate. four different types of anodes (nf, nf/pani, nf/pani/tc, nf/pani/tc/chit) are thus prepared and used in batch type microbial fuel cells operated with a mixed consortium of acetobacter aceti and gluconobacter roseus as the biocatalysts and bad wine as a feedstock. a maximum power density o ...	2016	26970695
review: diversity of microorganisms in global fermented foods and beverages.	culturalable and non-culturable microorganisms naturally ferment majority of global fermented foods and beverages. traditional food fermentation represents an extremely valuable cultural heritage in most regions, and harbors a huge genetic potential of valuable but hitherto undiscovered strains. holistic approaches for identification and complete profiling of both culturalable and non-culturable microorganisms in global fermented foods are of interest to food microbiologists. the application of ...	2016	27047484
fermented chinese formula shuan-tong-ling protects brain microvascular endothelial cells against oxidative stress injury.	fermented chinese formula shuan-tong-ling (stl), composed of fourteen medicinal herbs, was an experiential formula by dr. zhigang mei for treating vascular encephalopathy, but the underlying mechanisms remained unknown. in this study, we aimed to investigate the protective effects of fermented stl on hydrogen peroxide- (h2o2-) induced injury in rat brain microvascular endothelial cells (bmecs) and the possible mechanisms. cultured bmecs were treated with h2o2, stl, or nicotinamide (nam, a sirt1 ...	2016	28096886
vinegar production from jabuticaba (myrciaria jaboticaba) fruit using immobilized acetic acid bacteria.	cell immobilization comprises the retention of metabolically active cells inside a polymeric matrix. in this study, the production of jabuticaba (myrciaria jaboticaba) vinegar using immobilized acetobacter aceti and gluconobacter oxydans cells is proposed as a new method to prevent losses of jabuticaba fruit surplus. the pulp of jabuticaba was processed and saccharomyces cerevisiae ccma 0200 was used to ferment the must for jabuticaba wine production. sugars, alcohols (ethanol and glycerol) and ...	2016	27956867
microbial production of short chain fatty acids from lignocellulosic biomass: current processes and market.	biological production of organic acids from conversion of biomass derivatives has received increased attention among scientists and engineers and in business because of the attractive properties such as renewability, sustainability, degradability, and versatility. the aim of the present review is to summarize recent research and development of short chain fatty acids production by anaerobic fermentation of nonfood biomass and to evaluate the status and outlook for a sustainable industrial produc ...	2016	27556042
pulque, a traditional mexican alcoholic fermented beverage: historical, microbiological, and technical aspects.	pulque is a traditional mexican alcoholic beverage produced from the fermentation of the fresh sap known as aguamiel (mead) extracted from several species of agave (maguey) plants that grow in the central mexico plateau. currently, pulque is produced, sold and consumed in popular districts of mexico city and rural areas. the fermented product is a milky white, viscous, and slightly acidic liquid beverage with an alcohol content between 4 and 7° gl and history of consumption that dates back to pr ...	2016	27446061
effects of kefir on the cardiac autonomic tones and baroreflex sensitivity in spontaneously hypertensive rats.	it has been previously shown that the probiotic kefir (a symbiotic matrix containing acid bacteria and yeasts) attenuated the hypertension and the endothelial dysfunction in spontaneously hypertensive rats (shr). in the present study, the effect of chronic administration of kefir on the cardiac autonomic control of heart rate (hr) and baroreflex sensitivity (brs) in shr was evaluated.	2016	27375490
design and performance testing of a dna extraction assay for sensitive and reliable quantification of acetic acid bacteria directly in red wine using real time pcr.	although strategies exist to prevent aab contamination, the increased interest for wines with low sulfite addition leads to greater aab spoilage. hence, there is a real need for a rapid, specific, sensitive, and reliable method for detecting these spoilage bacteria. all these requirements are met by real time polymerase chain reaction (or quantitative pcr; qpcr). here, we compare existing methods of isolating dna and their adaptation to a red wine matrix. two different protocols for isolating dn ...	2016	27313572
functional dissection of the bipartite active site of the class i coenzyme a (coa)-transferase succinyl-coa:acetate coa-transferase.	coenzyme a (coa)-transferases catalyze the reversible transfer of coa from acyl-coa thioesters to free carboxylates. class i coa-transferases produce acylglutamyl anhydride intermediates that undergo attack by coa thiolate on either the internal or external carbonyl carbon atoms, forming distinct tetrahedral intermediates <3 å apart. in this study, crystal structures of succinyl-coa:acetate coa-transferase (aarc) from acetobacter aceti are used to examine how the asn347 carboxamide stabilizes th ...	2016	27242998
functional properties of microorganisms in fermented foods.	fermented foods have unique functional properties imparting some health benefits to consumers due to presence of functional microorganisms, which possess probiotics properties, antimicrobial, antioxidant, peptide production, etc. health benefits of some global fermented foods are synthesis of nutrients, prevention of cardiovascular disease, prevention of cancer, gastrointestinal disorders, allergic reactions, diabetes, among others. the present paper is aimed to review the information on some fu ...	2016	27199913
bacteria isolated from korean black raspberry vinegar with low biogenic amine production in wine.	a high concentration of histamine, one of the biogenic amines (bas) usually found in fermented foods, can cause undesirable physiological side effects in sensitive humans. the objective of this study is to isolate indigenous acetobacter strains from naturally fermented bokbunja vinegar in korea with reduced histamine production during starter fermentation. further, we examined its physiological and biochemical properties, including ba synthesis. the obtained strain mba-77, identified as acetobac ...	2016	26991285
16s rrna in situ hybridization followed by flow cytometry for rapid identification of acetic acid bacteria involved in submerged industrial vinegar production.	acetic acid bacteria are involved in many biotechnological processes such as vitamin c, gluconic acid, miglitol or acetic acid production, and others. for a technologist trying to control the industrial process, the ability to follow the microbiological development of the process is thus of importance. during the past few years hybridization in a combination with flow cytometry has often been used for this purpose. since vinegar is a liquid, it is an ideal matrix for flow cytometry analysis. in ...	2016	27904400
comparison of cultivable acetic acid bacterial microbiota in organic and conventional apple cider vinegar.	organic apple cider vinegar is produced from apples that go through very restricted treatment in orchard. during the first stage of the process, the sugars from apples are fermented by yeasts to cider. the produced ethanol is used as a substrate by acetic acid bacteria in a second separated bioprocess. in both, the organic and conventional apple cider vinegars the ethanol oxidation to acetic acid is initiated by native microbiota that survived alcohol fermentation. we compared the cultivable ace ...	2016	27904401
metabolite profiling and volatiles of pineapple wine and vinegar obtained from pineapple waste.	vinegar is an inexpensive commodity, and economic considerations require that a relatively low-cost raw material be used for its production. an investigation into the use of a new, alternative substrate - pineapple waste - is described. this approach enables the utilization of the pineapple's (ananas comosus) peels and core, which are usually discarded during the processing or consumption of the fruit. using physical and enzymatic treatments, the waste was saccharified, and the resulting substra ...	2017	28372238