Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| degradation of chloroaromatics: purification and characterization of maleylacetate reductase from pseudomonas sp. strain b13. | maleylacetate reductase of pseudomonas sp. strain b13 was purified to homogeneity by chromatography on deae-cellulose, butyl-sepharose, blue-sepharose, and sephacryl s100. the final preparation gave a single band by polyacrylamide gel electrophoresis under denaturing conditions and a single symmetrical peak by gel filtration under nondenaturing conditions. the subunit m(r) value was 37,000 (determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis). estimation of the native m(r) va ... | 1993 | 8407778 |
| purification and properties of the physically associated meta-cleavage pathway enzymes 4-hydroxy-2-ketovalerate aldolase and aldehyde dehydrogenase (acylating) from pseudomonas sp. strain cf600. | the final two steps in the dmp operon-encoded meta-cleavage pathway for phenol degradation in pseudomonas sp. strain cf600 involve conversion of 4-hydroxy-2-ketovalerate to pyruvate and acetyl coenzyme a (acetyl-coa) by the enzymes 4-hydroxy-2-ketovalerate aldolase and aldehyde dehydrogenase (acylating) [acetaldehyde:nad+ oxidoreductase (coa acetylating), ec 1.2.1.10]. a procedure for purifying these two enzyme activities to homogeneity is reported here. the two activities were found to copurify ... | 1993 | 8419288 |
| oxidation of biphenyl by a multicomponent enzyme system from pseudomonas sp. strain lb400. | pseudomonas sp. strain lb400 grows on biphenyl as the sole carbon and energy source. this organism also cooxidizes several chlorinated biphenyl congeners. biphenyl dioxygenase activity in cell extract required addition of nad(p)h as an electron donor for the conversion of biphenyl to cis-2,3-dihydroxy-2,3-dihydrobiphenyl. incorporation of both atoms of molecular oxygen into the substrate was shown with 18o2. the nonlinear relationship between enzyme activity and protein concentration suggested t ... | 1993 | 8419290 |
| degradation of 2-chloroallylalcohol by a pseudomonas sp. | three pseudomonas strains capable of utilizing 2-chloroallylalcohol (2-chloropropenol) as the sole carbon source for growth were isolated from soil. the fastest growth was observed with strain jd2, with a generation time of 3.6 h. degradation of 2-chloroallylalcohol was accompanied by complete dehalogenation. chloroallylalcohols that did not support growth were dechlorinated by resting cells; the dechlorination level was highest if an alpha-chlorine substituent was present. crude extracts of str ... | 1993 | 8434917 |
| characterization of the structural gene encoding a copper-containing nitrite reductase and homology of this gene to dna of other denitrifiers. | a copper-containing nitrite reductase gene (niru) from pseudomonas sp. strain g-179 was found in a 1.9-kb ecori-bamhi dna fragment. the coding region contained information for a polypeptide of 379 amino acids. the encoded protein had 78% identity in amino acid sequence to the nitrite reductase purified from achromobacter cycloclastes. the ligands for type 1 copper- and type 2 copper-binding sites found in a. cycloclastes were also found in pseudomonas sp. strain g-179, suggesting that these bind ... | 1993 | 8439151 |
| cloning and nucleotide sequence of the gene encoding the positive regulator (dmpr) of the phenol catabolic pathway encoded by pvi150 and identification of dmpr as a member of the ntrc family of transcriptional activators. | the catabolic plasmid pvi150 of pseudomonas sp. strain cf600 encodes all the genetic information required for the regulated metabolism of phenol and some of its methyl-substituted derivatives. the structural dmp genes of the pathway are clustered in a single operon that lies just downstream of a -24 tggc, -12 ttgc nif/ntr-like promoter sequence. promoters of this class are recognized by a minor form of rna polymerase utilizing sigma 54 (ntra, rpon). primer extension analysis demonstrated that th ... | 1993 | 8449869 |
| metabolism of 2,2'-dihydroxybiphenyl by pseudomonas sp. strain hbp1: production and consumption of 2,2',3-trihydroxybiphenyl. | cells of pseudomonas sp. strain hbp1 grown on 2-hydroxy- or 2,2'-dihydroxybiphenyl contain nadh-dependent monooxygenase activity that hydroxylates 2,2'-dihydroxybiphenyl. the product of this reaction was identified as 2,2',3-trihydroxybiphenyl by 1h nuclear magnetic resonance and mass spectrometry. furthermore, the monooxygenase activity also hydroxylates 2,2',3-trihydroxybiphenyl at the c-3' position, yielding 2,2',3,3'-tetrahydroxybiphenyl as a product. an estradiol ring cleavage dioxygenase a ... | 1993 | 8449871 |
| cloning and characterization of pseudomonas sp. strain dnt genes for 2,4-dinitrotoluene degradation. | the degradation of 2,4-dinitrotoluene (dnt) by pseudomonas sp. strain dnt is initiated by a dioxygenase attack to yield 4-methyl-5-nitrocatechol (mnc) and nitrite. subsequent oxidation of mnc by a monooxygenase results in the removal of the second molecule of nitrite, and further enzymatic reactions lead to ring fission. initial studies on the molecular basis of dnt degradation in this strain revealed the presence of three plasmids. mitomycin-derived mutants deficient in either dnt dioxygenase o ... | 1993 | 8449889 |
| construction of a pseudomonas hybrid strain that mineralizes 2,4,6-trinitrotoluene. | a bacterium, pseudomonas sp. strain c1s1, able to grow on 2,4,6-trinitrotoluene (tnt), 2,4- and 2,6-dinitrotoluene, and 2-nitrotoluene as n sources, was isolated. the bacterium grew at 30 degrees c with fructose as a c source and accumulated nitrite. through batch culture enrichment, we isolated a derivative strain, called pseudomonas sp. clone a, which grew faster on tnt and did not accumulate nitrite in the culture medium. use of tnt by these two strains as an n source involved the successive ... | 1993 | 8468288 |
| selection of pseudomonas sp. strain hbp1 prp for metabolism of 2-propylphenol and elucidation of the degradative pathway. | a mutant of pseudomonas sp. strain hbp1, originally isolated on 2-hydroxybiphenyl, was selected for the ability to grow on 2-propylphenol as the sole carbon and energy source. in the mutant strain, which was designated as pseudomonas sp. strain hbp1 prp, the cellular induction mechanism involved in the synthesis of the nadh-dependent monooxygenase is changed. 2-propylphenol, which is known to be a substrate of the monooxygenase, does not induce formation of the monooxygenase in the wild type but ... | 1993 | 8481010 |
| n.m.r. spectroscopic studies of fucose-containing oligosaccharides derived from keratanase digestion of articular cartilage keratan sulphates. influence of fucose residues on keratanase cleavage. | keratan sulphate chains from bovine articular cartilage were fully digested with keratanase from pseudomonas sp. and the products were reduced with alkaline borohydride. the resultant fragments were fractionated on a nucleosil 5sb column and the earliest eluting fucose-containing oligosaccharides were isolated. structural analysis using 1h n.m.r. spectroscopy (600 mhz) showed the two least-charged species to have the following structure: glcnac(6s) beta 1-3gal beta 1-4(fuc alpha 1-3)glcnac(6s) b ... | 1993 | 8489515 |
| developmental regulation of the gene for formate dehydrogenase in neurospora crassa. | we have isolated and characterized a gene, fdh, from neurospora crassa which is developmentally regulated and which produces formate dehydrogenase activity when expressed in escherichia coli. the gene is closely linked (less than 0.6 kb apart) to the leu-5 gene encoding mitochondrial leucyl-trna synthetase; the two genes are transcribed convergently from opposite strands. the expression patterns of these genes differ: fdh mrna is found only during conidiation and early germination and is not det ... | 1993 | 8509325 |
| microbial oxidation of dimethylnaphthalene isomers. | three bacterial strains, identified as alcaligenes sp. strain d-59 and pseudomonas sp. strains d-87 and d-186, capable of growing on 2,6-dimethylnaphthalene (2,6-dmn) as the sole source of carbon and energy were isolated from soil samples. 2,6-naphthalene dicarboxylic acid was formed in the culture broths of these three strains grown on 2,6-dmn. in addition, 2-hydroxymethyl-6-methylnaphthalene and 6-methylnaphthalene-2-carboxylic acid were detected in the culture broth of strain d-87. strain d-8 ... | 1993 | 8517744 |
| physiological factors affecting carbon tetrachloride dehalogenation by the denitrifying bacterium pseudomonas sp. strain kc. | pseudomonas sp. strain kc was grown on a medium with a low content of transition metals in order to examine the conditions for carbon tetrachloride (ct) transformation. several carbon sources, including acetate, glucose, glycerol, and glutamate, were able to support ct transformation. the chelators 2,2'-dipyridyl and 1,10-phenanthroline stimulated ct transformation in a rich medium that otherwise did not support this activity. low (< 10 microm) additions of dissolved iron(ii), iron(iii), and cob ... | 1993 | 8517754 |
| kinetics of bacterial growth on chlorinated aliphatic compounds. | with the pure bacterial cultures ancylobacter aquaticus ad20 and ad25, xanthobacter autotrophicus gj10, and pseudomonas sp. strain ad1, monod kinetics was observed during growth in chemostat cultures on 1,2-dichloroethane (ad20, ad25, and gj10), 2-chloroethanol (ad20 and gj10), and 1,3-dichloro-2-propanol (ad1). both the michaelis-menten constants (k(m)) of the first catabolic (dehalogenating) enzyme and the monod half-saturation constants (k(s)) followed the order 2-chloroethanol, 1,3-dichloro- ... | 1993 | 16348981 |
| cloning, sequence analysis, and expression of the flavobacterium pentachlorophenol-4-monooxygenase gene in escherichia coli. | the pcpb gene of flavobacterium sp. strain atcc 39723 was cloned by using a degenerate primer designed from the n-terminal sequence of the purified enzyme. the nucleotide sequence of pcpb was determined and found to encode an open reading frame of 1,614 nucleotides, yielding a predicted translation product of 538 amino acids, in agreement with the estimated size of the purified protein analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the transcriptional start of pcpb was fo ... | 1993 | 7678243 |
| pseudomonas sp. strain hbp1 prp degrades 2-isopropylphenol (ortho-cumenol) via meta cleavage. | pseudomonas sp. strain hbp1 prp grew on 2-isopropylphenol as the sole carbon and energy source with a maximal specific growth rate of 0.14 h-1 and transient accumulation of isobutyric acid. oxygen uptake experiments with resting cells and enzyme assays with crude-cell extracts showed that 2-isopropylphenol was catabolized via a broad-spectrum meta cleavage pathway. these findings were confirmed by experiments with partially purified enzymes. identification of 3-isopropylcatechol and 2-hydroxy-6- ... | 1994 | 7811094 |
| use of molecular techniques to evaluate the survival of a microorganism injected into an aquifer. | a pcr primer set and an internal probe that are specific for pseudomonas sp. strain b13, a 3-chlorobenzoate-metabolizing strain, were developed. using this primer set and probe, we were able to detect pseudomonas sp. strain b13 dna sequences in dna extracted from aquifer samples 14.5 months after pseudomonas sp. strain b13 had been injected into a sand and gravel aquifer. this primer set and probe were also used to analyze isolates from 3-chlorobenzoate enrichments of the aquifer samples by sout ... | 1994 | 7912498 |
| regiospecific and stereoselective hydroxylation of 1-indanone and 2-indanone by naphthalene dioxygenase and toluene dioxygenase. | the biotransformation of 1-indanone and 2-indanone to hydroxyindanones was examined with bacterial strains expressing naphthalene dioxygenase (ndo) and toluene dioxygenase (tdo) as well as with purified enzyme components. pseudomonas sp. strain 9816/11 cells, expressing ndo, oxidized 1-indanone to a mixture of 3-hydroxy-1-indanone (91%) and 2-hydroxy-1-indanone (9%). the (r)-3-hydroxy-1-indanone was formed in 62% enantiomeric excess (ee) (r:s, 81:19), while the 2-hydroxy-1-indanone was racemic. ... | 1994 | 7944365 |
| comparative studies of genes encoding thermostable l-2-halo acid dehalogenase from pseudomonas sp. strain yl, other dehalogenases, and two related hypothetical proteins from escherichia coli. | we have determined the nucleotide sequence of the gene encoding thermostable l-2-halo acid dehalogenase (l-dex) from the 2-chloroacrylate-utilizable bacterium pseudomonas sp. strain yl. the open reading frame consists of 696 nucleotides corresponding to 232 amino acid residues. the protein molecular weight was estimated to be 26,179, which was in good agreement with the subunit molecular weight of the enzyme. the gene was efficiently expressed in the recombinant escherichia coli cells: the amoun ... | 1994 | 7944368 |
| biodegradation of 2-nitrotoluene by pseudomonas sp. strain js42. | a strain of pseudomonas sp. was isolated from nitrobenzene-contaminated soil and groundwater on 2-nitrotoluene as the sole source of carbon, energy, and nitrogen. bacterial cells growing on 2-nitrotoluene released nitrite into the growth medium. the isolate also grew on 3-methylcatechol, 4-methylcatechol, and catechol. 2-nitrotoluene, 3-methylcatechol, and catechol stimulated oxygen consumption by intact cells regardless of the growth substrate. crude extracts from the isolate contained catechol ... | 1994 | 7944378 |
| initial reactions in the anaerobic oxidation of toluene and m-xylene by denitrifying bacteria. | pseudomonas sp. strain t and pseudomonas sp. strain k172 grow with toluene under denitrifying conditions. we demonstrated that anaerobic degradation of toluene was initiated by direct oxidation of the methyl group. benzaldehyde and benzoate accumulated sequentially after toluene was added when cell suspensions were incubated at 5 degrees c. strain t also grows anaerobically with m-xylene, and we demonstrated that degradation was initiated by oxidation of one methyl group. in cell suspensions inc ... | 1994 | 7993091 |
| oxidative release of nitrite from 2-nitrotoluene by a three-component enzyme system from pseudomonas sp. strain js42. | pseudomonas sp. strain js42 utilizes 2-nitrotoluene (2nt) as the sole source of carbon and energy for growth. intact cells catalyze the oxidation of 2nt to 3-methylcatechol and nitrite in a reaction that requires molecular oxygen. cell extracts oxidized 2nt to 3-methylcatechol and nitrite in the presence of nad(p)h and ferrous iron. ion-exchange chromatography yielded three protein fractions (a, b, and c) which were all required for the oxidation of 2nt to 3-methylcatechol and nitrite. component ... | 1994 | 8002568 |
| an aromatic effector specificity mutant of the transcriptional regulator dmpr overcomes the growth constraints of pseudomonas sp. strain cf600 on para-substituted methylphenols. | the pvi150 catabolic plasmid of pseudomonas sp. strain cf600 carries the dmp system, which comprises the divergently transcribed dmpr gene and the dmp operon coding for the catabolic enzymes required for growth on (methyl)phenols. the constitutively expressed dmpr transcriptional activator positively controls the expression of the rpon-dependent dmp operon promoter in the presence of the aromatic effector in the growth medium. however, the magnitude of the transcriptional response differs depend ... | 1994 | 8002579 |
| organization and evolution of naphthalene catabolic pathways: sequence of the dna encoding 2-hydroxychromene-2-carboxylate isomerase and trans-o-hydroxybenzylidenepyruvate hydratase-aldolase from the nah7 plasmid. | the sequence of a 2,437-bp dna segment from the naphthalene upper catabolic pathway operon of plasmid nah7 was determined. this segment contains three large open reading frames designated nahq', nahe, and nahd. the first of these is the 3' end of an open reading frame that has no known function, the second (993 bp) encodes trans-o-hydroxybenzylidenepyruvate hydratase-aldolase (deduced molecular weight, 36,640), and the third (609 bp) encodes 2-hydroxychromene-2-carboxylate isomerase (deduced mol ... | 1994 | 8002605 |
| genetic organization of the mau gene cluster in methylobacterium extorquens am1: complete nucleotide sequence and generation and characteristics of mau mutants. | the nucleotide sequence of the methylamine utilization (mau) gene region from methylobacterium extorquens am1 was determined. open reading frames for 11 genes (maufbedacjglmn) were found, all transcribed in the same orientation. the maub, maua, and mauc genes encode the periplasmic methylamine dehydrogenase (madh) large and small subunit polypeptides and amicyanin, respectively. the products of maud, maug, maul, and maum were also predicted to be periplasmic. the products of mauf, maue, and maun ... | 1994 | 8021187 |
| nucleotide sequence and functional analysis of the meta-cleavage pathway involved in biphenyl and polychlorinated biphenyl degradation in pseudomonas sp. strain kks102. | pseudomonas sp. strain kks102 is able to degrade biphenyl and polychlorinated biphenyls via the meta-cleavage pathway. we sequenced the upstream region of the bpha1a2a3bcd (open reading frame 1 [orf1]) a4 and found four orfs in this region. as the deduced amino acid sequences of the first, second, and third orfs are homologous to the meta-cleavage enzymes from pseudomonas sp. strain cf600 (v. shingler, j. powlowski, and u. marklund, j. bacteriol. 174:711-724, 1992), these orfs have been named bp ... | 1994 | 8021212 |
| inability of muconate cycloisomerases to cause dehalogenation during conversion of 2-chloro-cis,cis-muconate. | the conversion of 2-chloro-cis,cis-muconate by muconate cycloisomerase from pseudomonas putida prs2000 yielded two products which by nuclear magnetic resonance spectroscopy were identified as 2-chloro- and 5-chloromuconolactone. high-pressure liquid chromatography analyses showed the same compounds to be formed also by muconate cycloisomerases from acinetobacter calcoaceticus adp1 and pseudomonas sp. strain b13. during 2-chloro-cis,cis-muconate turnover by the enzyme from p. putida, 2-chloromuco ... | 1994 | 8021223 |
| cross-regulation by xylr and dmpr activators of pseudomonas putida suggests that transcriptional control of biodegradative operons evolves independently of catabolic genes. | the pu promoter of the toluene degradation plasmid pww0 of pseudomonas putida drives expression of an operon involved in the sequential oxidation of toluene and m- and p-xylenes to benzoate and toluates, respectively. similarly, the po promoter of plasmid pvi150 controls expression of an operon of pseudomonas sp. strain cf600 which is required for the complete catabolism of phenol and cresols. these promoters, which both belong to the sigma 54-dependent class, are regulated by their cognate acti ... | 1994 | 8051017 |
| degradation of alkylphenol ethoxylates by pseudomonas sp. strain tr01. | an alkylphenol ethoxylate-degrading bacterium was isolated from activated sludge of a municipal sewage treatment plant by enrichment culture. this organism was found to belong to the genus pseudomonas; since no corresponding species was identified, we designated it as pseudomonas sp. strain tr01. this strain had an optimal temperature and ph of 30 degrees c and 7, respectively, for both growth and the degradation of triton n-101 (a nonylphenol ethoxylate in which the average number of ethylene o ... | 1994 | 8074508 |
| purification and characterization of thermostable and nonthermostable 2-haloacid dehalogenases with different stereospecificities from pseudomonas sp. strain yl. | two novel hydrolytic dehalogenases, thermostable l-2-haloacid dehalogenase (l-dex) inducibly synthesized by 2-chloropropionate (2-cpa) and nonthermostable dl-2-haloacid dehalogenase (dl-dex) induced by 2-chloroacrylate, were purified to homogeneity from pseudomonas sp. strain yl. dl-dex consisted of a monomer with a molecular weight of about 36,000 and catalyzed the dehalogenation of l and d isomers of 2-cpa to produce d- and l-lactates, respectively. it acted on 2-haloalkanoic acids with a carb ... | 1994 | 8074519 |
| evidence for a novel pathway in the degradation of fluorene by pseudomonas sp. strain f274. | a fluorene-utilizing microorganism, identified as a species of pseudomonas, was isolated from soil severely contaminated from creosote use and was shown to accumulate six major metabolites from fluorene in washed-cell incubations. five of these products were identified as 9-fluorenol, 9-fluorenone, (+)-1,1a-dihydroxy-1-hydro-9-fluorenone, 8-hydroxy-3,4-benzocoumarin, and phthalic acid. this last compound was also identified in growing cultures supported by fluorene. fluorene assimilation into ce ... | 1994 | 8074523 |
| azoreductase activity in bacteria associated with the greening of instant chocolate puddings. | pseudomonas sp. strain azr1 and klebsiella sp. strain azr2 were isolated from reconstituted instant chocolate puddings that had turned green and were found to have azoreductase activity. this activity was inducible and nadh dependent. differences in dye reduction rates between the two strains were apparent, and substrate specificity related to dye structure was observed. | 1994 | 8085839 |
| insertion sequence is6100 on plasmid poad2, which degrades nylon oligomers. | the nucleotide sequence of repeated sequence i, which appears in five regions on nylon oligomer-degrading plasmid poad2, harbored in flavobacterium sp. strain k172, was determined. the five regions of repeated sequence i had 880 bp of identical sequence, and the sequence was identical to that of is6100, an insertion sequence classified in the is6 family, initially found in mycobacterium fortuitum. sequences homologous to that of is6100 were found for another nylon oligomer-degrading plasmid, pna ... | 1994 | 8106333 |
| identification and characterization of the treponema pallidum tpn50 gene, an ompa homolog. | treponema pallidum is a pathogenic spirochete that has no known genetic exchange mechanisms. in order to identify treponemal genes encoding surface and secreted proteins, we carried out tnphoa mutagenesis of a t. pallidum genomic dna library in escherichia coli. several of the resulting clones expressed enzymatically active t. pallidum-alkaline phosphatase fusion proteins. the dna sequence of the 5' portion of a number of the treponemal genes was obtained and analyzed. a recombinant clone harbor ... | 1994 | 8112835 |
| sensing of aromatic compounds by the dmpr transcriptional activator of phenol-catabolizing pseudomonas sp. strain cf600. | the dmp operon of the pvi150 catabolic plasmid of pseudomonas sp. strain cf600 encodes the enzymes involved in the catabolism of phenol and methylphenols. the regulator of this dmp pathway, dmpr, is a member of the ntrc family of transcriptional activators and controls transcription of the dmp operon in response to aromatic effector compounds (v. shingler, m. bartilson, and t. moore, j. bacteriol. 175:1596-1604, 1993). using a lux gene fusion reporter system, in which the dmpr-regulated operon p ... | 1994 | 8132448 |
| identification of the bpha4 gene encoding ferredoxin reductase involved in biphenyl and polychlorinated biphenyl degradation in pseudomonas sp. strain kks102. | the nucleotide sequence of the downstream region of the bph operon from pseudomonas sp. strain kks102 was determined. two open reading frames (orf1 and orf2) were found in this region, and the deduced amino acid sequence of orf2 showed homology with the sequences of four ferredoxin reductases of dioxygenase systems. when this region was inserted just upstream of the bph operon, which does not contain a gene encoding ferredoxin reductase, biphenyl dioxygenase activity was detected. the 24- and 44 ... | 1994 | 8132464 |
| analysis of the binding site of the lysr-type transcriptional activator tcbr on the tcbr and tcbc divergent promoter sequences. | the tcbr transcriptional activator protein, which is encoded by the tcbr gene of pseudomonas sp. strain p51 (j. r. van der meer, a. c. j. frijters, j. h. j. leveau, r. i. l. eggen, a. j. b. zehnder, and w. m. de vos, j. bacteriol. 173:3700-3708, 1991), was purified from overproducing escherichia coli cells by using a two-step chromatographic procedure. subsequent use of tcbr in gel mobility shift assays with progressively shortened portions of a dna fragment containing the divergent promoter seq ... | 1994 | 8144450 |
| metabolism of both 4-chlorobenzoate and toluene under denitrifying conditions by a constructed bacterial strain. | t1, a dentrifying bacterium originally isolated for its ability to grow on toluene, can also metabolize 4-hydroxybenzoate and other aromatic compounds under denitrifying conditions. a cosmid clone carrying the three genes that code for the 4-chlorobenzoate dehalogenase enzyme complex isolated from the aerobic bacterium pseudomonas sp. strain cbs3 was successfully conjugated into strain t1. the cloned enzyme complex catalyzes the hydrolytic dechlorination of 4-chlorobenzoate to 4-hydroxybenzoate. ... | 1994 | 8161190 |
| biodegradation of 4-methyl-5-nitrocatechol by pseudomonas sp. strain dnt. | pseudomonas sp. strain dnt degrades 2,4-dinitrotoluene (dnt) by a dioxygenase attack at the 4,5 position with concomitant removal of the nitro group to yield 4-methyl-5-nitrocatechol (mnc). here we describe the mechanism of removal of the nitro group from mnc and subsequent reactions leading to ring fission. washed suspensions of dnt-grown cells oxidized mnc and 2,4,5-trihydroxytoluene (tht). extracts prepared from dnt-induced cells catalyzed the disappearance of mnc in the presence of oxygen an ... | 1994 | 8195105 |
| a biological sensor for iron available to bacteria in their habitats on plant surfaces. | a sensor responsive to iron was constructed by fusing a promoterless ice nucleation activity gene (inaz) to an iron-regulated promoter of a genomic region involved in pyoverdine (fluorescent siderophore) (pvd) production in pseudomonas syringae. cells of pseudomonas fluorescens and p. syringae that contained the pvd-inaz fusion expressed iron-responsive ice nucleation activity in the bean rhizosphere and phyllosphere, respectively, and in culture. addition of fe(iii) to leaves or soil reduced th ... | 1994 | 16349283 |
| quantification of frankia strains and other root-associated bacteria in pure cultures and in the rhizosphere of axenic seedlings by high-performance liquid chromatography-based muramic acid assay. | application of a high-performance liquid chromatography-based muramic acid assay with precolumn fluorescence derivatization to quantification of root-associated bacteria was studied both in pure cultures and in the rhizosphere of axenic festuca rubra seedlings. quantities of muramic acid from acid-hydrolyzed cells of frankia strains, streptomyces griseoviridis, enterobacter agglomerans, klebsiella pneumoniae, pseudomonas sp., and bacillus polymyxa were mostly proportional to the respective cell ... | 1994 | 16349413 |
| localization and characterization of the carbon tetrachloride transformation activity of pseudomonas sp. strain kc. | previous research has established that pseudomonas sp. strain kc rapidly transforms carbon tetrachloride (ct) to carbon dioxide (45 to 55%), a nonvolatile fraction (45 to 55%), and a cell-associated fraction ((equiv)5%) under denitrifying, iron-limited conditions. the present study provides additional characterization of the nonvolatile fraction, demonstrates that electron transfer plays a role in the transformation, and establishes the importance of both extracellular and intracellular factors. ... | 1995 | 16534941 |
| isolation and characterization of a pseudomonas sp. that mineralizes the s-triazine herbicide atrazine. | a bacterium that was capable of metabolizing atrazine at very high concentrations (>1,000 ppm) was isolated from a herbicide spill site. the organism was differentiated by observing clearing zones on indicator agar plates containing 1,000 ppm atrazine. detailed taxonomic studies identified the organism as a pseudomonas sp., designated adp, that was dissimilar to currently known species. pseudomonas sp. strain adp metabolized atrazine as its sole nitrogen source. nongrowing suspended cells also m ... | 1995 | 16534995 |
| transformation of substituted fluorenes and fluorene analogs by pseudomonas sp. strain f274. | pseudomonas sp. strain f274, previously shown to catabolize fluorene via fluorenone and its angular dioxygenation, 2(prm1),3(prm1)-dihydroxy-2-carboxybiphenyl, phthalate, and protocatechuate, was examined for its ability to transform substituted fluorenes and s- and n-heterocyclic analogs. halogen- and methyl-substituted fluorenes were metabolized to correspondingly substituted phthalates via attack on the unsubstituted ring. in the case of 1-methylfluorene, initial oxidation of the methyl group ... | 1995 | 16535132 |
| uptake of benzoic acid and chloro-substituted benzoic acids by alcaligenes denitrificans bri 3010 and bri 6011. | the mechanism of uptake of benzoic and 2,4-dichlorobenzoic acid (2,4-dcba) by alcaligenes denitrificans bri 3010 and bri 6011 and pseudomonas sp. strain b13, three organisms capable of degrading various isomers of chlorinated benzoic acids, was investigated. in all three organisms, uptake of benzoic acid was inducible. for benzoic acid uptake into bri 3010, monophasic saturation kinetics with apparent k(infm) and v(infmax) values of 1.4 (mu)m and 3.2 nmol/min/mg of cell dry weight, respectively, ... | 1995 | 16535175 |
| dihydroxylation and dechlorination of chlorinated biphenyls by purified biphenyl 2,3-dioxygenase from pseudomonas sp. strain lb400. | oxidation of biphenyl and nine chlorinated biphenyls (cbs) by the biphenyl 2,3-dioxygenase from pseudomonas sp. strain lb400 was examined. the purified terminal oxygenase required the addition of partially purified electron transport components, nad(p)h, and ferrous iron to oxidize biphenyl and cbs. cis-biphenyl 2,3-dihydrodiol was produced with biphenyl as the substrate. dihydrodiols were produced from all cbs, and more than one compound was produced with most substrates. catechols were produce ... | 1995 | 8002618 |
| maleylacetate reductase of pseudomonas sp. strain b13: specificity of substrate conversion and halide elimination. | maleylacetate reductase (ec 1.3.1.32) plays a major role in the degradation of chloroaromatic compounds by channelling maleylacetate and some chlorinated derivatives into the 3-oxoadipate pathway. several substituted maleylacetates were prepared in situ by alkaline or enzymatic hydrolysis of dienelactones as the precursor. the conversion of these methyl-, chloro-, fluoro-, and bromo-substituted maleylacetates by malelacetate reductase from 3-chlorobenzoate-grown cells of pseudomonas sp. strain b ... | 1995 | 7814320 |
| lipase modulator protein (liml) of pseudomonas sp. strain 109. | plasmids containing a pseudomonas sp. strain 109 extracellular lipase gene (lipl) lacking nh2-terminal sequence and a lipase modulator gene (liml) lacking the nh2-terminal hydrophobic region were constructed and expressed independently in escherichia coli by using the t7 promoter expression vector system. recombinant lipl (rlipl) was produced as inclusion bodies, whereas recombinant liml (rliml) was present as a soluble protein. during in vitro renaturation of the purified rlipl inclusion bodies ... | 1995 | 7868599 |
| aromatic effector activation of the ntrc-like transcriptional regulator phhr limits the catabolic potential of the (methyl)phenol degradative pathway it controls. | pseudomonas putida p35x (ncib 9869) metabolizes phenol and monomethylphenols via a chromosomally encoded meta-cleavage pathway. we have recently described a 13.4-kb fragment of the chromosome that codes for the first eight genes of the catabolic pathway and a divergently transcribed positive regulator, phhr. the eight structural genes lie in an operon, the phh operon, downstream of a -24 tggc, -12 ttgc promoter sequence. promoters of this class are recognized by rna polymerase that utilizes the ... | 1995 | 7883704 |
| transformation of carbon tetrachloride via sulfur and oxygen substitution by pseudomonas sp. strain kc. | pseudomonas sp. strain kc transforms carbon tetrachloride into carbon dioxide and nonvolatile products, without chloroform as an intermediate. to define the pathway for hydrolysis, nonvolatile products were analyzed. condensation products containing the carbon atom of carbon tetrachloride as carbonyl and thioxo moieties were identified, indicating the intermediacy of phosgene and thiophosgene in the pathway. | 1995 | 7721711 |
| desaturation, dioxygenation, and monooxygenation reactions catalyzed by naphthalene dioxygenase from pseudomonas sp. strain 9816-4. | the stereospecific oxidation of indan and indene was examined with mutant and recombinant strains expressing naphthalene dioxygenase of pseudomonas sp. strain 9816-4. pseudomonas sp. strain 9816/11 and escherichia coli jm109(de3)[pdtg141] oxidized indan to (+)-(1s)-indanol, (+)-cis-(1r,2s)-indandiol, (+)-(1s)-indenol, and 1-indanone. the same strains oxidized indene to (+)-cis-(1r,2s)-indandiol and (+)-(1s)-indenol. purified naphthalene dioxygenase oxidized indan to the same four products formed ... | 1995 | 7751268 |
| characterization of biphenyl catabolic genes of gram-positive polychlorinated biphenyl degrader rhodococcus sp. strain rha1. | rhodococcus sp. strain rha1 is a gram-positive polychlorinated biphenyl (pcb) degrader which can degrade 10 ppm of pcb48 (equivalent to aroclor1248), including tri-, tetra-, and pentachlorobiphenyls, in a few days. we isolated the 7.6-kb ecori-bamhi fragment carrying the biphenyl catabolic genes of rha1 and determined their nucleotide sequence. on the basis of deduced amino acid sequence homology, we identified six bph genes, bpha1a2a3a4, bphb, and bphc, that are responsible for the initial thre ... | 1995 | 7793929 |
| survival and activity of pseudomonas sp. strain b13(fr1) in a marine microcosm determined by quantitative pcr and an rrna-targeting probe and its effect on the indigenous bacterioplankton. | genetically engineered pseudomonas sp. strain b13(fr1) was released into laboratory-scale marine ecosystem models (microcosms). survival of the introduced population in the water column and the sediment was determined by plating on a selective medium and by quantitative competitive pcr. the activity of the released bacteria was determined by in situ hybridization of single cells with a specific rrna-targeting oligonucleotide probe. two microcosms were inoculated with 10(6) cells ml-1, while an u ... | 1995 | 7538271 |
| bacterial degradation of m-nitrobenzoic acid. | pseudomonas sp. strain js51 grows on m-nitrobenzoate (m-nba) with stoichiometric release of nitrite. m-nba-grown cells oxidized m-nba and protocatechuate but not 3-hydroxybenzoate, 4-hydroxy-3-nitrobenzoate, 4-nitrocatechol, and 1,2,4-benzenetriol. protocatechuate accumulated transiently when succinate-grown cells were transferred to media containing m-nba. respirometric experiments indicated that the conversion of m-nba to protocatechuate required 1 mol of oxygen per mol of substrate. conversio ... | 1995 | 7574625 |
| nucleotide sequence analysis of genes encoding a toluene/benzene-2-monooxygenase from pseudomonas sp. strain js150. | it was previously shown by others that pseudomonas sp. strain js150 metabolizes benzene and alkyl- and chloro-substituted benzenes by using dioxygenase-initiated pathways coupled with multiple downstream metabolic pathways to accommodate catechol metabolism. by cloning genes encoding benzene-degradative enzymes, we found that strain js150 also carries genes for a toluene/benzene-2-monooxygenase. the gene cluster encoding a 2-monooxygenase and its cognate regulator was cloned from a plasmid carri ... | 1995 | 7574644 |
| cloning, characterization, and expression of a gene region from pseudomonas sp. strain adp involved in the dechlorination of atrazine. | we previously identified a pseudomonas sp. strain, adp, which rapidly metabolized atrazine in liquid culture, agar plates, and soils (r. t. mandelbaum, d. l. allan, l. p. wackett, appl. environ. microbiol. 61:1451-1457, 1995). in this study, we report the cloning and partial characterization of a gene region from pseudomonas sp. strain adp that encodes atrazine degradation activity. a 22-kb ecori genomic dna fragment, designated pmd1, was shown to encode atrazine dechlorination activity in esche ... | 1995 | 7574646 |
| induction of toluene oxidation activity in pseudomonas mendocina kr1 and pseudomonas sp. strain envpc5 by chlorinated solvents and alkanes. | toluene oxidation activity in pseudomonas mendocina kr1 and pseudomonas sp. strain envpc5 was induced by trichloroethylene (tce), and induction was followed by the degradation of tce. higher levels of toluene oxidation activity were achieved in the presence of a supplemental growth substrate such as glutamate, with levels of activity of up to 86% of that observed with toluene-induced cells. activity in p. mendocina kr1 was also induced by cis-1,2-dichloroethylene, perchloroethylene, chloroethane ... | 1995 | 7574658 |
| desaturation and oxygenation of 1,2-dihydronaphthalene by toluene and naphthalene dioxygenase. | bacterial strains expressing toluene and naphthalene dioxygenase were used to examine the sequence of reactions involved in the oxidation of 1,2-dihydronaphthalene. toluene dioxygenase of pseudomonas putida f39/d oxidizes 1,2-dihydronaphthalene to (+)-cis-(1s,2r)-dihydroxy-1,2,3,4-tetrahydronaphthalene, (+)-(1r)-hydroxy-1,2-dihydronaphthalene, and (+)-cis-(1r,2s)-dihydroxy-1,2-dihydronaphthalene. in contrast, naphthalene dioxygenase of pseudomonas sp. strain ncib 9816/11 oxidizes 1,2-dihydronaph ... | 1995 | 7592326 |
| purification and characterization of the oxygenase component of biphenyl 2,3-dioxygenase from pseudomonas sp. strain lb400. | the iron-sulfur protein of biphenyl 2,3-dioxygenase (ispbph) was purified from pseudomonas sp. strain lb400. the protein is composed of a 1:1 ratio of a large (alpha) subunit with an estimated molecular weight of 53,300 and a small (beta) subunit with an estimated molecular weight of 27,300. the native molecular weight was 209,000, indicating that the protein adopts an alpha 3 beta 3 native conformation. measurements of iron and acid-labile sulfide gave 2 mol of each per mol of alpha beta hetero ... | 1995 | 7592331 |
| evidence that operons tcb, tfd, and clc encode maleylacetate reductase, the fourth enzyme of the modified ortho pathway. | the maleylacetate reductase from pseudomonas sp. strain b13 functioning in the modified ortho pathway was purified and digested with trypsin. the polypeptides separated by high-performance liquid chromatography were sequenced. alignments with the polypeptides predicted from the tfdf and tcbf genes located on plasmids pjp4 of the 2,4-dichlorophenoxyacetate-degrading alcaligenes eutrophus jmp134 and pp51 of the 1,2,4-trichlorobenzene-degrading pseudomonas sp. strain p51 as well as polypeptides pre ... | 1995 | 7601858 |
| conversion of chlorobiphenyls into phenylhexadienoates and benzoates by the enzymes of the upper pathway for polychlorobiphenyl degradation encoded by the bph locus of pseudomonas sp. strain lb400. | metabolism of 21 chlorobiphenyls by the enzymes of the upper biphenyl catabolic pathway encoded by the bph locus of pseudomonas sp. strain lb400 was investigated by using recombinant strains harboring gene cassettes containing bphabc or bphabcd. the enzymes of the upper pathway were generally able to metabolize mono- and dichlorinated biphenyls but only partially transform most trichlorinated congeners investigated: 14 of 15 mono- and dichlorinated and 2 of 6 trichlorinated congeners were conver ... | 1995 | 7618878 |
| transformation of low concentrations of 3-chlorobenzoate by pseudomonas sp. strain b13: kinetics and residual concentrations. | the transformation of 3-chlorobenzoate (3cb) and acetate at initial concentrations in the wide range of 10 nm to 16 mm was studied in batch experiments with pseudomonas sp. strain b13. transformation rates of 3cb at millimolar concentrations could be described by michaelis-menten kinetics (k(infm), 0.13 mm; v(infmax), 24 nmol (middot) mg of protein(sup-1) (middot) min(sup-1)). experiments with nanomolar and low micromolar concentrations of 3cb indicated the possible existence of two different tr ... | 1996 | 16535232 |
| atropine metabolism by pseudomonas sp. strain at3: evidence for nortropine as an intermediate in tropine breakdown and reactions leading to succinate. | pseudomonas strain at3, isolated by elective culture with atropine, hydrolyzed atropine and grew diauxically, first on the tropic acid and then on the tropine. tropine was also used as a sole carbon and energy source. the methyl group of tropine was eliminated as formaldehyde, and the nortropine thus formed was a precursor of 6-hydroxycyclohepta-1,4-dione. ammonia was detected as a product of nitrogen elimination. 6-hydroxycyclohepta-1,4-dione was oxidized to cyclohepta-1,3,5-trione by an induce ... | 1996 | 16535398 |
| maleylacetate reductases in chloroaromatic-degrading bacteria using the modified ortho pathway: comparison of catalytic properties. | the maleylacetate reductases from pseudomonas aeruginosa rho1 and alcaligenes eutrophus jmp134 were tested for activity and affinity to various maleylacetates as well as dechlorinating properties. the dechlorinating activity and the kcat/km values revealed high-level similarity of these reductases to that of pseudomonas sp. strain b13. | 1996 | 8550433 |
| genetic structures of the genes encoding 2,3-dihydroxybiphenyl 1,2-dioxygenase and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase from biphenyl- and 4-chlorobiphenyl-degrading pseudomonas sp. strain dj-12. | the pcbc and pcbd genes of pseudomonas sp. strain dj-12, a natural isolate degrading biphenyl and 4-chlorobiphenyl, encode the 2,3-dihydroxybiphenyl 1,2-dioxygenase and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase, respectively. the two genes were sequenced and appear to be present in the order pcbd-pcbc as an operon. | 1996 | 8572703 |
| a novel dipeptidyl aminopeptidase from pseudomonas sp. strain wo24. | an activity similar to that of dipeptidyl aminopeptidase i (dap i) which releases dipeptide from gly-arg-p-nitroanilide (gly-arg-pna) was detected in a pseudomonas sp. an enzyme was isolated and purified about 400-fold by a series of column chromatographies. the enzyme, named dap bi (dap from bacteria, type i), was revealed to be homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and isoelectric focusing. the molecular mass was estimated to be 82 kda by sds-page ... | 1996 | 8631703 |
| catechol 2,3-dioxygenases functional in oxygen-limited (hypoxic) environments. | we studied the degradation of toluene for bacteria isolated from hypoxic (i.e., oxygen-limited) petroleum-contaminated aquifers and compared such strains with other toluene degraders. three pseudomonas isolates, p. pickettii pko1, pseudomonas sp. strain w31, and p. fluorescens cfs215, grew on toluene when nitrate was present as an alternate electron acceptor in hypoxic environments. we examined kinetic parameters (k(m) and vmax) for catechol 2,3-dioxygenase (c230), a key shared enzyme of the tol ... | 1996 | 8633871 |
| stereospecific dihydroxylation of the styrene vinyl group by purified naphthalene dioxygenase from pseudomonas sp. strain ncib 9816-4. | naphthalene dioxygenase (ndo) from pseudomonas sp. strain ncib 9816-4 adds both atoms of the dioxygen molecule to styrene to form (r)-l-phenyl-1,2-ethanediol. product formation is tightly coupled to dioxygen consumption and nadh oxidation. ndo oxidizes styrene-d8 at almost the same initial rate as styrene. the results indicate that dioxygen activation by ndo is different from that by cytochrome p-450 and other monooxygenases, which oxidize styrene to styrene 1,2-oxide. | 1996 | 8655521 |
| chloroform mineralization by toluene-oxidizing bacteria. | seven toluene-oxidizing bacterial strains (pseudomonas mendocina kr1, burkholderia cepacia g4, pseudomonas putida f1, pseudomonas pickettii pko1, and pseudomonas sp. strains envpc5, envbf1, and env113) were tested for their ability to degrade chloroform (cf). the greatest rate of cf oxidation was achieved with strain envbf1 (1.9 nmol/min/mg of cell protein). cf also was oxidized by p. mendocina kr1 (0.48 nmol/min/mg of cell protein), strain envpc5 (0.49 nmol/min/mg of cell protein), and escheric ... | 1996 | 8702263 |
| atrazine chlorohydrolase from pseudomonas sp. strain adp: gene sequence, enzyme purification, and protein characterization. | pseudomonas sp. strain adp metabolizes atrazine to carbon dioxide and ammonia via the intermediate hydroxyatrazine. the genetic potential to produce hydroxyatrazine was previously attributed to a 1.9-kb avai dna fragment from strain adp (m. l. de souza, l. p. wackett, k. l. boundy-mills, r. t. mandelbaum, and m. j. sadowsky, appl. environ. microbiol. 61:3373-3378, 1995). in this study, sequence analysis of the 1.9-kb avai fragment indicated that a single open reading frame, atza, encoded an acti ... | 1996 | 8759853 |
| toluene and ethylbenzene oxidation by purified naphthalene dioxygenase from pseudomonas sp. strain ncib 9816-4. | purified naphthalene dioxygenase (ndo) from pseudomonas sp. strain ncib 9816-4 oxidized toluene to benzyl alcohol and benzaldehyde by reactions involving benzylic monooxygenation and dioxygen-dependent alcohol oxidation, respectively. xylene and nitrotoluene isomers were also oxidized to substituted benzyl alcohol and benzaldehyde derivatives. ndo oxidized ethylbenzene sequentially through (s)-1-phenethyl alcohol (77% enantiomeric excess) and acetophenone to 2-hydroxyacetophenone. in addition, n ... | 1996 | 8795196 |
| phenotypic and genotypic characterization of phenanthrene-degrading fluorescent pseudomonas biovars. | a total of 41 phenanthrene degraders were isolated from a former coal gasification site by using pseudomonas-selective gould's s1 medium. all isolates were found to belong to the fluorescent pseudomonas group and were subjected to characterization by phenotypic methods, including classical taxonomic tests, api 20ne, and biolog gn, and the strains were further characterized by the genotypic method repetitive extragenic palindromic pcr (rep-pcr). by using classical tests, the population was found ... | 1996 | 8837438 |
| genetic exchange in soil between introduced chlorobenzoate degraders and indigenous biphenyl degraders. | pseudomonas aeruginosa jb2, a chlorobenzoate degrader, was inoculated into soil having indigenous biphenyl degraders but no identifiable 2-chlorobenzoate (2cba) or 2,5-dichlorobenzoate (2,5dcba) degraders. the absence of any indigenous chlorobenzoate degraders was noted by the failure to obtain enrichment cultures with the addition of 2cba, 3cba, or 2,5dcba and by the failure of soil dna to hybridize to the tfdc gene, which encodes ortho fission of chlorocatechols. in contrast, dna extracted fro ... | 1996 | 8837452 |
| two types of novel dipeptidyl aminopeptidases from pseudomonas sp. strain wo24. | two kinds of dipeptidyl aminopeptidase i (dap i [cathepsin c])-like activities which hydrolyze gly-phe-p-nitroanilide (gly-phe-pna) were detected in pseudomonas sp. strain wo24. they were purified and characterized. the isolated enzymes, named dap bii and dap biii, were revealed to be homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and isoelectric focusing. dap bii was estimated to have a molecular mass of 150,000 da by gel filtration and a subunit size of 73, ... | 1996 | 8892831 |
| cloning, sequencing, and expression of isopropylbenzene degradation genes from pseudomonas sp. strain jr1: identification of isopropylbenzene dioxygenase that mediates trichloroethene oxidation. | pseudomonas sp. strain jr1, recently isolated with isopropylbenzene (ipb) as the inducer substrate for trichloroethene (tce) oxidation (b. dabrock, j. riedel, j. bertram, and g. gottschalk, arch. microbiol 158:9-13, 1992), is able to degrade ipb via the meta-cleavage pathway. the genes encoding the first three enzymes in the catabolism of isopropylbenzene were isolated from a genomic library with the broad-host-range cosmid vector pwe15. a 7.6-kb fragment from a 37.7-kb primary cosmid clone was ... | 1996 | 8899984 |
| regio- and stereospecific oxidation of fluorene, dibenzofuran, and dibenzothiophene by naphthalene dioxygenase from pseudomonas sp. strain ncib 9816-4. | the regio- and stereospecific oxidation of fluorene, dibenzofuran, and dibenzothiophene was examined with mutant and recombinant strains expressing naphthalene dioxygenase from pseudomonas sp. strain ncib 9816-4. the initial oxidation products were isolated and identified by gas chromatography-mass spectrometry and nuclear magnetic resonance spectrometry. salicylate-induced cells of pseudomonas sp. strain 9816/11 and isopropyl-beta-d-thiogalactopyranoside-induced cells of escherichia coli jm109( ... | 1996 | 8899998 |
| oxidation of 6,7-dihydro-5h-benzocycloheptene by bacterial strains expressing naphthalene dioxygenase, biphenyl dioxygenase, and toluene dioxygenase yields homochiral monol or cis-diol enantiomers as major products. | bacterial strains expressing naphthalene, biphenyl, and toluene dioxygenase were examined for their abilities to oxidize 6,7-dihydro-5h-benzocycloheptene (benzocyclohept-1-ene). the major oxidation products were isolated, and their absolute configurations were determined by chiral 1h nuclear magnetic resonance analysis of diastereomeric boronate esters, chiral stationary-phase high-pressure liquid chromatography, and stereo-chemical correlation. pseudomonas sp. strain 9816/11 and sphingomonas ya ... | 1996 | 8919798 |
| measurement of minimum substrate concentration (smin) in a recycling fermentor and its prediction from the kinetic parameters of pseudomonas strain b13 from batch and chemostat cultures. | the minimum substrate concentration required for growth, smin, was measured for pseudomonas sp. strain b13 with 3-chlorobenzoate (3cb) and acetate in a recycling fermentor. the substrates were provided alone or in a mixture. smin values predicted with kinetic parameters from resting-cell batches and chemostat cultures differed clearly from the values measured in the recycling fermentor. when 3cb and acetate were fed as single substrates, the measured smin values were higher than the individual s ... | 1996 | 8967775 |
| comparison of factors influencing trichloroethylene degradation by toluene-oxidizing bacteria. | the degradation of trichloroethylene (tce) by toluene-oxidizing bacteria has been extensively studied, and yet the influence of environmental conditions and physiological characteristics of individual strains has received little attention. to consider these effects, the levels of tce degradation by strains distinguishable on the basis of toluene and nitrate metabolism were compared under aerobic or hypoxic conditions in the presence and absence of nitrate and an exogenous electron donor, lactate ... | 1996 | 8975612 |
| purification of an extracellular d-(-)-3-hydroxybutyrate oligomer hydrolase from pseudomonas sp. strain a1 and cloning and sequencing of its gene. | an extracellular d-(-)-3-hydroxybutyrate oligomer hydrolase was purified from a poly(3-hydroxybutyrate)-degrading bacterium, pseudomonas sp. strain a1. the purified enzyme hydrolyzed the d-(-)-3-hydroxybutyrate dimer and trimer at similar rates. the enzyme activity was inhibited by a low concentration of diisopropylfluorophosphate. the molecular weight of the hydrolase was estimated to be about 70,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. a 10-kbp dna fragment of a1 was d ... | 1997 | 8981982 |
| enzymology of oxidation of tropic acid to phenylacetic acid in metabolism of atropine by pseudomonas sp. strain at3. | pseudomonas sp. strain at3 grew with dl-tropic acid, the aromatic component of the alkaloid atropine, as the sole source of carbon and energy. tropic acid-grown cells rapidly oxidized the growth substrate, phenylacetaldehyde, and phenylacetic acid. crude cell extracts, prepared from dl-tropic acid-grown cells, contained two nad+-linked dehydrogenases which were separated by ion-exchange chromatography and shown to be specific for their respective substrates, dl-tropic acid and phenylacetaldehyde ... | 1997 | 9023182 |
| the atzb gene of pseudomonas sp. strain adp encodes the second enzyme of a novel atrazine degradation pathway. | we previously reported the isolation of a 21.5-kb genomic dna fragment from pseudomonas sp. strain adp, which contains the atza gene, encoding the first metabolic step for the degradation of the herbicide atrazine (m. de souza, l. p. wackett, k. l. boundy-mills, r. t. mandelbaum, and m. j. sadowsky, appl. environ. microbiol. 61:3373-3378, 1995). in this study, we show that this fragment also contained the second gene of the atrazine metabolic pathway, atzb. atzb catalyzed the transformation of h ... | 1997 | 9055410 |
| heterologous expression of biphenyl dioxygenase-encoding genes from a gram-positive broad-spectrum polychlorinated biphenyl degrader and characterization of chlorobiphenyl oxidation by the gene products. | the bpha1a2a3a4 gene cluster, encoding a biphenyl dioxygenase from rhodococcus globerulus p6, a gram-positive microorganism able to degrade a wide spectrum of polychlorobiphenyls (pcbs), was expressed in pseudomonas putida, thereby allowing characterization of chlorobiphenyl oxidation by this enzyme. while p6 biphenyl dioxygenase activity was observed in p. putida containing bpha1a2a3a4, no activity was detected in escherichia coli cells containing the same gene cluster. in e. coli, transcriptio ... | 1997 | 9068637 |
| molecular characterization of genes of pseudomonas sp. strain hr199 involved in bioconversion of vanillin to protocatechuate. | the gene loci vdh, vana, and vanb, which are involved in the bioconversion of vanillin to protocatechuate by pseudomonas sp. strain hr199 (dsm 7063), were identified as the structural genes of a novel vanillin dehydrogenase (vdh) and the two subunits of a vanillate demethylase (vana and vanb), respectively. these genes were localized on an ecori fragment (e230), which was cloned from a pseudomonas sp. strain hr199 genomic library in the cosmid pvk100. the vdh gene was identified on a subfragment ... | 1997 | 9098058 |
| preparation of gm1 ganglioside with sialidase-producing marine bacteria as a microbial biocatalyst. | this paper describes the preparation of monosialoganglioside gm1 with sialidase-producing marine bacteria as a microbial biocatalyst. a new sialidase-producing bacterium, identified tentatively as pseudomonas sp. strain yf-2, was isolated from seawater by enrichment culture with ganglioside as the sole source of carbon. when yf-2 was cultured in a synthetic medium containing crude bovine brain gangliosides at 25 degrees c for 3 days, 80 to 90% of the gangliosides were converted to gm1. gm1 was t ... | 1997 | 9143118 |
| stereospecific oxidation of (r)- and (s)-1-indanol by naphthalene dioxygenase from pseudomonas sp. strain ncib 9816-4. | a recombinant escherichia coli strain which expresses naphthalene dioxygenase (ndo) from pseudomonas sp. strain ncib 9816-4 oxidized (s)-1-indanol to trans-(1s,3s)-indan-1,3-diol (95.5%) and (r)-3-hydroxy-1-indanone (4.5%). the same cells oxidized (r)-1-indanol to cis-1,3-indandiol (71%), (r)-3-hydroxy-1-indanone (18.2%), and cis-1,2,3-indantriol (10.8%). purified ndo oxidized (s)-1-indenol to both syn- and anti-2,3-dihydroxy-1-indanol. | 1997 | 9143136 |
| structure and expression of a pyrimidine gene cluster from the extreme thermophile thermus strain zo5. | on a 4.7-kbp hindiii clone of thermus strain zo5 dna, complementing an aspartate carbamoyltransferase mutation in escherichia coli, we identified a cluster of four potential open reading frames corresponding to genes pyrr, and pyrb, an unidentified open reading frame named bbc, and gene pyrc. the transcription initiation site was mapped at about 115 nucleotides upstream of the pyrr translation start codon. the cognate thermus pyr promoter also functions in heterologous expression of thermus pyr ... | 1997 | 9171389 |
| cloning, characterization, and sequence analysis of the clce gene encoding the maleylacetate reductase of pseudomonas sp. strain b13. | a 3,167-bp psti fragment of genomic dna from pseudomonas sp. strain b13 was cloned and sequenced. the gene clce consists of 1,059 nucleotides encoding a protein of 352 amino acids with a calculated mass of 37,769 da which showed maleylacetate reductase activity. the protein had significant sequence similarities with the polypeptides encoded by tcbf of pp51 (59.4% identical positions), tfdf of pjp4 (55.1%), and tfte of burkholderia cepacia ac1100 (53.1%). the function of tcbf as maleylacetate red ... | 1997 | 9171435 |
| gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase. | the gene encoding 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (mhpco; ec 1.14.12.4) was cloned by using an oligonucleotide probe corresponding to the n terminus of the enzyme to screen a dna library of pseudomonas sp. ma-1. the gene encodes for a protein of 379 amino acid residues corresponding to a molecular mass of 41.7 kda, the same as that previously estimated for mhpco. mhpco was expressed in escherichia coli and found to have the same properties as the native enzyme from pseudom ... | 1997 | 9207074 |
| bacterial dl-2-haloacid dehalogenase from pseudomonas sp. strain 113: gene cloning and structural comparison with d- and l-2-haloacid dehalogenases. | dl-2-haloacid dehalogenase from pseudomonas sp. strain 113 (dl-dex) catalyzes the hydrolytic dehalogenation of both d- and l-2-haloalkanoic acids to produce the corresponding l- and d-2-hydroxyalkanoic acids, respectively, with inversion of the c2 configuration. dl-dex is a unique enzyme: it acts on the chiral carbon of the substrate and uses both enantiomers as equivalent substrates. we have isolated and sequenced the gene encoding dl-dex. the open reading frame consists of 921 bp corresponding ... | 1997 | 9209038 |
| cloning of genes involved in carbazole degradation of pseudomonas sp. strain ca10: nucleotide sequences of genes and characterization of meta-cleavage enzymes and hydrolase. | the dna fragment encoding meta-cleavage enzymes and the meta-cleavage compound hydrolase, involved in carbazole degradation, was cloned from the carbazole-utilizing bacterium pseudomonas sp. strain ca10. dna sequence analysis of this 2.6-kb smai-sphi fragment revealed that there were three open reading frames (orf1, orf2, and orf3, in this gene order). orf1 and orf2 were indispensable for meta-cleavage activity for 2'-aminobiphenyl-2,3-diol and its easily available analog, 2,3-dihydroxybiphenyl, ... | 1997 | 9244273 |
| identification and characterization of genes encoding carbazole 1,9a-dioxygenase in pseudomonas sp. strain ca10. | nucleotide sequence analysis of the flanking regions of the carbc genes of pseudomonas sp. strain ca10 revealed that there were two open reading frames (orfs) orf4 and orf5, in the upstream region of carbc. similarly, three orfs, orf6 to orf8, were found in the downstream region of carbc. the deduced amino acid sequences of orf6 and orf8 showed homologies with ferredoxin and ferredoxin reductase components of bacterial multicomponent dioxygenase systems, respectively. orf4 and orf5 had the same ... | 1997 | 9244274 |
| use of a promoterless lacz gene insertion to investigate chitinase gene expression in the marine bacterium pseudoalteromonas sp. strain s9. | sequence data for genes encoding 16s rrna indicated that the marine strain previously named pseudomonas sp. strain s9 would be better identified as a pseudoalteromonas sp. by use of transposon mutagenesis, a chitinase-negative mutant of s9 with a lacz reporter gene insertion was isolated. part of the interrupted gene was cloned and sequenced. the deduced amino acid sequence had homology to sequences of bacterial chitinases. expression of the chitinase gene promoter was quantified by measuring th ... | 1997 | 9251187 |
| cross-regulation of toluene monooxygenases by the transcriptional activators tbmr and tbut. | the toluene-3-monooxygenase from burkholderia pickettii pko1 and the toluene/benzene-2-monooxygenase from burkholderia (pseudomonas) sp. strain js150 are distinct enzymes which differ not only in catalytic specificity and substrate range but also in the arrangement and sequence of the genes within the operons that encode the enzymes, tbua1ubva2c and tbmabcdef, respectively. in the present study, we examined the transcriptional activation of the ptbua1 and ptbma promoters by their cognate regulat ... | 1997 | 9293027 |
| multiple pathways for toluene degradation in burkholderia sp. strain js150. | burkholderia (pseudomonas) sp. strain js150 uses multiple pathways for the metabolism of catechols that result from degradation of aromatic compounds. this suggests that the strain also uses multiple upstream pathways for the initial hydroxylation of aromatic substrates. two distinct dna fragments that allowed pseudomonas aeruginosa pao1c to grow with benzene as a sole carbon source were cloned from strain js150. one of the recombinant plasmids containing the initial steps for the degradative pa ... | 1997 | 9327568 |
| chemotaxis of pseudomonas spp. to the polyaromatic hydrocarbon naphthalene. | two naphthalene-degrading bacteria, pseudomonas putida g7 and pseudomonas sp. strain ncib 9816-4, were chemotactically attracted to naphthalene in drop assays and modified capillary assays. growth on naphthalene or salicylate induced the chemotactic response. p. putida g7 was also chemotactic to biphenyl; other polyaromatic hydrocarbons that were tested did not appear to be chemoattractants for either pseudomonas strain. strains that were cured of the naphthalene degradation plasmid were not att ... | 1997 | 9327579 |
| comparative in vitro activities of trovafloxacin (cp-99,219) against 221 aerobic and 217 anaerobic bacteria isolated from patients with intra-abdominal infections. | four hundred thirty-eight bacteria cultured from specimens of patients with serious intra-abdominal infections were tested by agar dilution against trovafloxacin and other quinolones and antimicrobial agents. trovafloxacin inhibited 435 strains (99.3%) at < or =2 microg/ml. all the quinolones had similar activities against enterobacteriaceae and pseudomonas sp., but trovafloxacin showed superior activities against streptococci, enterococci, and anaerobic organisms. because of its excellent in vi ... | 1997 | 9333074 |
| microbiologically proven bacterial infections in aids. | we have reviewed the incidence, type and site of microbiologically proven bacterial infection occurring in 52 patients with the acquired immunodeficiency syndrome (aids) who presented to southmead hospital, bristol between 1990 and 1994. a total of 30 (58%) patients had significant bacterial isolates. the majority of infections were community acquired. overall, more infections were caused by gram-negative organisms but gram-positive organisms predominated in bacteraemia. mycobacterium avium intr ... | 1997 | 9373597 |
| effect of selected polysaccharide-producing soil bacteria on hyperhydricity control in oregano tissue cultures. | hyperhydricity, or vitrification, is a physiological malformation affecting tissue culture-generated plants. this malformation is associated with excessive hydration and poor lignification and results in poor regeneration of plants. we have tested hyperhydricity prevention in oregano by several nonspecific polysaccharide-producing rhizosphere bacteria. among these bacteria, pseudomonas mucidolens and another pseudomonas sp. prevented hyperhydricity and improved acclimation of oregano clones. the ... | 1997 | 16535525 |