Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| molecular cloning and nucleotide sequence of cdna for rat ornithine carbamoyltransferase precursor. | messenger rna of rat ornithine carbamoyltransferase (ec 2.1.3.3), a mitochondrial matrix enzyme, was enriched by immunoprecipitation of rat liver free polysomes, and recombinant plasmids were prepared from the enriched mrna by a vector-primer method. the cdna clones for ornithine carbamoyltransferase were identified by hybrid-arrested translation and hybrid-selected translation. one of the clones, designated potc-1, contained a 1.6-kilobase insert and hybridized to a mrna of approximately equal ... | 1984 | 6095294 |
| functions of human neutrophilic granulocytes after in vivo exposure to interferon alpha. | the ability of neutrophilic granulocytes to phagocytize yeast particles and to reduce nitro blue tetrazolium at rest and on activation with bacterial stimuli was monitored in 32 patients receiving treatment with human interferon alpha. the ability of these cells to attach to and ingest yeast particles was not altered to any major extent during 1 year of interferon treatment. in most patients, the nitro blue tetrazolium-reducing activity increased after the first injection of interferon. during p ... | 1984 | 6365781 |
| cloning and expression in escherichia coli of a yeast mannosyltransferase from the asparagine-linked glycosylation pathway. | the yeast saccharomyces cerevisiae temperature-sensitive lethal mutant alg1-1, has been previously shown to lack the activity necessary for the addition of the first mannose residue in the synthesis of lipid-linked precursor oligosaccharide. the gene alg1 has been cloned by complementation of the temperature-sensitive mutation alg1-1 with a total genomic dna library. the original dna fragment isolated was 11,300 base pairs and has been subcloned to a 1,500-base pair fragment which is still capab ... | 1984 | 6368538 |
| [morphogenesis of candida albicans in human serum. mycelial and pseudomycelial tubes observed in 250 strains]. | the morphogenesis of candida albicans, more peculiarly the mycelial and pseudomycelial tubes production, was investigated during 24 hours in human serum: on 250 strains at 37 degrees c, and on 24 strains at 25 degrees c. the characteristic of the tubes, their first apparition and maximum production time are reported. the yeast influence (origin, saprophytic or parasitic state, serotype, maximum temperature for growth of the strain) on the tubes production is discussed. | 1984 | 6371674 |
| fungistatic and fungicidal activity of human parotid salivary histidine-rich polypeptides on candida albicans. | human parotid saliva histidine-rich polypeptides exerted antifungal activity against candida albicans at concentrations similar to the known antifungal activity of the imidazole antibiotics. inhibition of both growth and viability could be demonstrated by optical density monitoring and plating assays. inhibition of growth was observed to be greatest when the histidine-rich polypeptides were added to the inoculum before addition to the growth media. however, complete inhibition by these polypepti ... | 1984 | 6373615 |
| human liver alcohol dehydrogenase: amino acid substitution in the beta 2 beta 2 oriental isozyme explains functional properties, establishes an active site structure, and parallels mutational exchanges in the yeast enzyme. | the homodimeric oriental beta 2 beta 2 isozyme of human liver alcohol dehydrogenase, corresponding to an allelic variant at the adh2 gene locus, was studied in order to define the amino acid exchange in relation to the beta 1 beta 1 isozyme, the predominant allelic form among caucasians. sequence analysis reveals that the amino acid substitution occurs at position 7 of the largest cnbr fragment, corresponding to position 47 of the whole protein chain. here, the beta 2 form has a histidine residu ... | 1984 | 6374651 |
| heterogeneity of human neutrophil phagolysosomes: functional consequences for candidacidal activity. | opsonized yeast phase candida albicans, incubated with human neutrophils, are internalized into two classes of phagosomes. one class, termed "unsealed vacuoles," comprises approximately 40% of the total and maintains functional communication to the cell's exterior that is sufficient to permit ingress of dyes, such as trypan or methylene blue. the remaining cell-associated yeasts are contained in "sealed vacuoles," completely sequestered from the external milieu. approximately 71.8% of c albicans ... | 1984 | 6375758 |
| isolation of glucose-containing high-mannose glycoprotein core oligosaccharides. | the total cell wall mannoprotein has been isolated from a mutant of saccharomyces cerevisiae that fails to remove the glucose units of the dolichol-linked precursor after transfer of the oligosaccharide to asparagine units in the protein. the oligosaccharides released from this mannoprotein by endoglucosaminidase h digestion show 1h nmr signals assignable to three alpha-linked glucose units as delta 5.52, 5.27, and 5.17, and a comparison with the chemical shifts of reference compounds shows that ... | 1984 | 6387703 |
| adherence of candida albicans to buccal and vaginal epithelial cells: ultrastructural observations. | the adherence of candida albicans to human buccal and vaginal epithelial cells was studied by transmission electron microscopy. adherence to epithelial cells was confirmed by both a radiometric assay as well as direct microscopic examination of stained cell preparations. ultramicroscopic preparations revealed that yeast cells were closely appressed to epithelial cell surfaces and were often partially enclosed within phagocyticlike invaginations of the epithelial cells. a murine model of vaginiti ... | 1984 | 6388767 |
| saccharomyces cerevisiae infections in man. | saccharomyces cerevisiae is a yeast commonly used to make food products such as bread, beer and wine, or ingested whole as a 'health' food. we report five cases of infections involving s. cerevisiae, including one in which s. cerevisiae alone was implicated, and review the literature on its pathogenicity. | 1984 | 6390738 |
| yeast as protein source for human nutrition (a review). | 1984 | 6393680 | |
| cytochemical and ultrastructural studies of candida albicans. iii. evidence for modifications of the cell wall coat during adherence to human buccal epithelial cells. | ultrastructural modifications of the cell wall coat of candida albicans during adherence to host cells were investigated using various cytochemical techniques. attachment of the fungus to buccal epithelial cells appeared to involve spatial rearrangement of their cell wall surface. in particular adhering yeast developed a fibrogranular surface layer visualized by the periodic acid--thiocarbohydrazide--silver proteinate technique (a polysaccharide detection technique); concanavalin a binding sites ... | 1984 | 6393889 |
| effect of amoxycillin and doxycycline on function of human granulocytes tested in vitro and on chemotaxis of granulocytes from rabbits given the two antibiotics. | the effect of amoxycillin and doxycycline on human granulocyte function was studied in vitro. the antimicrobial agents were added to tests of chemotaxis, random motility, yeast phagocytosis, and killing, at progressing concentrations. the effect was also evaluated in vivo by measuring chemotaxis and random motility (agarose technique) of granulocytes from rabbits injected with these antibiotics. amoxycillin was shown to have a slightly stimulating effect on chemotaxis, demonstrable only at the h ... | 1984 | 6394496 |
| [preventive measures for the control of fungal infections in the clinic]. | hospital acquired infections due to fungi are primarily caused by yeast species of the genus candida and mould species of the genus aspergillus. underlying disease with severely impaired defence mechanisms as well as certain forms of immunosuppressive and aggressive chemotherapy are the most important prerequisites for such secondary fungal infections. aspergillus spec. usually infect man via exogenous routes, whereas candida spec. mostly originate from the patient's own microbial flora. under c ... | 1984 | 6398797 |
| [comparative biological value of protein hydrolysates and their fortified forms]. | experiments on wistar male rats were made to study the biological value (with the use of the "growth" method) of basic and protein- and crystalline amino acids-enriched protein hydrolysates. autolysine and protein hydrolysates obtained from formed elements of the blood of slaughtered animals (the degree of degradation 30 and 70%) and a mixture of crystalline amino acids composed according to the fao/who scale were studied. casein was used as control. the differences in the biological value of ba ... | 1984 | 6147936 |
| plasmid vectors for positive galactose-resistance selection of cloned dna in escherichia coli. | insertion of a hindiii-ecori fragment carrying part of the gal operon from lambda gal+ into pbr322 yields a plasmid (paa3) which confers strong galactose sensitivity on e. coli strains deleted for the gal operon. sensitivity to galactose is caused by the expression of kinase and transferase (but not epimerase) genes from a promoter located in the tet gene of pbr322. insertion of a dna fragment carrying tn9 at the hindiii junction blocks gal expression and produces a galactose-resistant phenotype ... | 1984 | 6329914 |
| purification and characterization of a mannose-containing disaccharide obtained from human pregnancy urine. a new immunoregulatory saccharide. | endogenous mammalian lectin-like sugar-binding molecules have been previously described that have immunoregulatory properties. further, the addition of defined simple saccharides to lymphocyte cultures has been shown to inhibit a variety of in vitro lymphocyte functions, presumably because these sugars are able to compete with the binding of endogenous lectins to critical membrane receptors. in this report, we describe the isolation and characterization of a d-mannose-containing disaccharide in ... | 1984 | 6334714 |
| induction of neutrophilic differentiation of human promyelocytic leukemic cells by branched-chain carboxylic acid anticonvulsant drugs. | the anticonvulsant drug 1-methyl-1-cyclohexanecarboxylic acid ( mcca ) has been shown to cause maturation of murine neuroblastoma cells in vitro at concentrations that are pharmacologically achievable. hl-60 human promyelocytic leukemia cells cultured with this drug underwent a dose-dependent decrease in growth. similarly, neutrophilic differentiation, based on morphologic criteria and the acquisition of the ability to reduce nitroblue tetrazolium and phagocytose yeast, was observed. valproic ac ... | 1984 | 6427413 |
| role of hydrophobicity in adherence of gram negative bacteria to epithelial cells. | agglutination of yeast, human group a and guinea pig erythrocytes by multiple clinical isolates of e. coli, k. pneumoniae, and p. aeruginosa was investigated and correlated with hydrophobicity measurements of each bacterial strain. hydrophobicity of the isolates, as measured by hydrophobic interaction chromatography, was similarly correlated with in vitro adherence of the microorganisms to buccal epithelial cells. agglutination and adherence studies were done in the presence and absence of 0.046 ... | 1984 | 6428296 |
| hyaluronic acid enhances phagocytosis of human monocytes in vitro. | pretreatment of human blood monocytes with hyaluronic acid for 0.5 hr enhanced significantly their ingestion in vitro of yeast particles opsonized with complement. the enhancement of the phagocytic capacity was dose dependent and maximal in the range of 25-50 micrograms/ml. | 1984 | 6429186 |
| peptostreptococcus productus strain that grows rapidly with co as the energy source. | anaerobic bacteria were enriched with a sewage digestor sludge inoculum and a mineral medium supplemented with b-vitamins and 0.05% yeast extract and with a 50% co-30% n2-20% co2 (2 atm [202 kpa]) gas phase. microscopic observation revealed an abundance of gram-positive cocci, 1.0 by 1.4 micron, which occurred in pairs or chains. the coccus, strain u-1, was isolated by using roll tubes with co as the energy source. based on morphology, sugars fermented, fermentation products from glucose (h2, ac ... | 1984 | 6430231 |
| immunodiffusion studies on the antigens of histoplasma capsulatum: comparison of mycelial histoplasmin with the yeast phase reagent histolyn-cyl. | immunodiffusion assays were performed to determine if h and m antigens associated with mycelial histoplasmin were present in the yeast phase reagent, histolyn-cyl (h-cyl). neither h nor m antigens could be detected in h-cyl. however, when h-cyl was reacted against human histoplasmal sera a positive reaction was evidenced in all instances in which a response was elicited with a reference mycelial immunodiffusion reagent. | 1984 | 6441272 |
| phagolysosomal ph of human neutrophils. | human neutrophils sequester yeast cells or zymosan particles in two classes of phagocytic vacuoles: sealed and unsealed. the ph of sealed vacuoles was measured by a newly devised fluorometric procedure that used fluoresceinated zymosan particles, derived from saccharomyces cerevisiae, as a ph probe. five minutes after initiation of phagocytosis, sealed vacuoles were alkaline, exhibiting a ph of 7.80 +/- 0.19 (mean +/- sem). their acidity subsequently increased, so that the ph fell to 7.38 +/- 0. ... | 1984 | 6418236 |
| the biochemical and functional heterogeneity of circulating human plasma fibronectin. | human plasma fibronectin purified by affinity chromatography, consisted of homogeneous 215 kd protein subunits when assessed by sds polyacrylamide gel electrophoresis. on isoelectric focusing however, 5 separate fractions were present, with isoelectric points ranging from 5.6 to 6.1. isoelectric focusing and immunofixation of native plasma produced similar but not identical appearances. only 15% of the total plasma fibronectin opsonically stimulated the ingestion of saccharomyces cerevisiae by h ... | 1984 | 6712671 |
| a reference allergen preparation of the house dust mite d. pteronyssinus, produced from whole mite culture--a part of the das 76 study. comparison with allergen preparations from other raw materials. | a d. pteronyssinus whole culture allergen preparation contained 49 antigens as revealed by crossed immunoelectrophoresis (cie), using polyspecific rabbit antibodies. crossed radio-immunoelectrophoresis ( crie ) with sera from 30 patients revealed nine allergens, antigens 42, x, y and 23 (in rank order) showing the most frequent and intense ige-uptake. nine antigens originated from the culture medium (human dander + yeast), but none of these gave rise to specific ige-uptake. extremely few and wea ... | 1984 | 6731752 |
| pyrvinium pamoate lacks in vivo genotoxicity in the colon. | pyrvinium pamoate, a widely used anthelmintic drug, has been previously tested for genotoxicity with equivocal results. assays with bacterial or yeast test strains yielded positive results while in vitro tests with mammalian cells yielded negative results. in this study, the genotoxicity of pyrvinium was studied in vivo in the mouse colon, the therapeutic site of action of this agent. 1,2-dimethylhydrazine (dmh), a colon carcinogen, was tested simultaneously as a positive control. the colonic nu ... | 1984 | 6740678 |
| agglutination typing of vibrio anguillarum isolates from diseased fish and from the environment. | agglutinating activity was widely distributed among 101 vibrio anguillarum strains of different origin and three vibrio ordalii strains from salmonids. the spectrum of cells which were agglutinated comprised yeast cells and human (type o), poultry, guinea pig, and trout erythrocytes, whereas ovine, bovine, and tanned bovine erythrocytes were not affected. mannose-sensitive hemagglutination, mannose-resistant hemagglutination, and non-agglutinating strains were recognized. the three v. ordalii st ... | 1984 | 16346564 |
| intracellular growth and phagocytosis of blastomyces dermatitidis by monocyte-derived macrophages from previously infected and normal subjects. | blastomyces dermatitidis evokes responses of human cellular immunity typical of other intracellular fungal pathogens. differences in growth rates of intracellular blastomyces yeast and the differences in amounts of yeast phagocytized by macrophages were determined for macrophages derived from peripheral blood monocytes from 11 persons with treated blastomycosis and 11 normal, healthy persons. cellular immunity was examined by lymphocyte uptake of [3h]thymidine in response to a specific antigen o ... | 1985 | 3965594 |
| igg purification to measure the level of an iodinated thyroglobulin peptide, the 3,5,3',5' tetraiodo-l-tyrosyl-l-tyrosine in human serum. | antibodies reacting with 3,5,3',5' tetraiodo-l-tyrosyl-l-tyrosine (i2tyr-i2tyr) were elicited in rabbits by immunization with an oxidized yeast conjugate coupled with i2tyr-i2tyr. ion-exchange chromatography was used to purify immunoglobulins, in order to improve the specificity in measurement of i2tyr-i2tyr level in patient serum. igg binding capacity versus i2tyr-i2tyr was considerably increased after immunoglobulin purification. | 1985 | 3972086 |
| a role for branchpoints in splicing in vivo. | the nucleotides immediately surrounding intron/exon junctions of genes transcribed by rna polymerase b can be derived from 'consensus' sequences for donor and acceptor splice sites by only a few base changes. studies in vivo have underlined the importance of these junction nucleotides for splicing. in higher eukaryotes, no evidence has been found for specific internal intron sequences involved in splicing. however, the recent discovery that, in vitro, introns are excised in a lariat form where t ... | 1985 | 4000270 |
| (at)n is an interspersed repeat in the xenopus genome. | we have observed (at)34 and (at)23 tracts close to the coding sequences of the xenopus laevis tadpole alpha t1 and adult beta 1 globin genes, respectively. we show that (at)n sequences are found as interspersed repeats within the xenopus globin and histone gene loci. using (at)n co-polymer in filter hybridisation experiments we estimate that there are 10(4) (at)n tracts per haploid xenopus genome. hybridisation to genomic blots of dna from yeast, slime mold, trypanosome, fruit fly, salmon, chick ... | 1985 | 4054102 |
| selenium status of exclusively breast-fed infants as influenced by maternal organic or inorganic selenium supplementation. | a longitudinal dietary se supplementation study on lactating mothers was performed to determine the possibilities of improving the se status of exclusively breast-fed infants. a total of 200 mothers randomized into three groups received either no se supplements, 100 micrograms of selenite, or 100 micrograms of yeast-se daily. maternal and infant serum se concentrations showed a linear correlation during exclusive breast-feeding. yeast-se in the dose administered was safe and more effective than ... | 1985 | 4061344 |
| isolation of yersinia enterocolitica and related species from foods in france. | the occurrence of yersinia enterocolitica and related species (y. intermedia, y. frederiksenii, y. kristensenii) in foods from france was investigated by using different enrichment procedures. initially, seven procedures were evaluated with pork products. these methods included a cold preenrichment in yeast extract-rose bengal broth or in phosphate-sorbitol-bile medium, followed by selective enrichment either in pastone-sucrose-tris-azide broth, in modified rappaport broth, or in bile-oxalate-so ... | 1985 | 4083878 |
| inhibition of bone marrow colony formation by human natural killer cells and by natural killer cell-derived colony-inhibiting activity. | incubation of human peripheral blood lymphocytes with bone marrow cells resulted in significant inhibition of colony formation by committed myeloid and erythroid cells. using positively selected homogeneous natural killer (nk) cell preparations and lymphocyte subpopulations depleted of or enriched for nk cells, we definitively characterize as nk cells the cells in normal peripheral blood that are responsible for inhibition of bone marrow colony growth. the inhibitory effect of nk cells on hemato ... | 1985 | 3838767 |
| [study on the presence of yeasts in the feces of the rock pigeon (columba livia gmelin 1789) from rural areas]. | the diffusion of rock-pigeon (domestic form of columba livia gmelin 1789) is greatly increasing owing to its high reproductivity; for this reason it is present in both countries and cities. this bird is considered an important vector of pathogens to man and domestic animals, and today it represents a serious hygienic-sanitary problem. therefore we checked for the presence of yeasts in feces of rock-pigeons nesting in the regional park "la mandria" (near turin), visited mostly by school-children ... | 1985 | 3870648 |
| modulation of human natural killer cell activity by recombinant human interleukin 2. | recombinant human il-2, secreted by yeast harboring a plasmid containing a synthetic il-2 gene, is biologically active in augmenting human natural killer (nk) cell activity. a dose-dependent linear stimulation of nk activity was obtained against the chronic myelogenous leukemia cell line k562 over the range 3 to 300 units/ml of il-2. enhancement of nk activity was similarly demonstrable against the less nk-sensitive carcinoma cell lines lovo and skosc. il-2 could also be demonstrated to augment ... | 1985 | 3871664 |
| modulation of human natural killer cell activity by recombinant human interleukin 2. | recombinant human il-2, secreted by yeast harboring a plasmid containing a synthetic il-2 gene, is biologically active in augmenting human natural killer (nk) cell activity. a dose-dependent linear stimulation of nk activity was obtained against the chronic myelogenous leukemia cell line k562 over the range of 3 to 300 units/ml of il-2. enhancement of nk activity was similarly demonstrable against the less nk-sensitive carcinoma cell lines lovo and skosc. il-2 could also be demonstrated to augme ... | 1985 | 3881192 |
| a flow cytometric method for the measurement of phagocytosis by polymorphonuclear leucocytes. | a new method for the measurement of phagocytosis of candida albicans by human polymorphonuclear leucocytes (pmn) is described using a fluorescence activated cell sorter. we have used acridine orange to discriminate between pmn which have internalised yeast particles and those which have not. this method allows accurate measurement of particle phagocytosis as an event distinct from particle adherence. it also permits detailed examination of the kinetics of phagocytosis, the study of which is like ... | 1985 | 3882836 |
| attempted expression of a human initiator trna gene in saccharomyces cerevisiae. | in attempts to overproduce the wild type and, eventually, mutant human initiator methionine trnas for use in structure-function relationship studies, we have investigated the expression of the wild type human initiator trna gene in the yeast saccharomyces cerevisiae, both in vitro and in vivo. we find that the yeast extract, while capable of accurately transcribing several yeast trna genes, does not transcribe the human initiator trna gene. in addition, when the human initiator trna gene is intr ... | 1985 | 3886659 |
| a novel ras-related gene family. | we have identified a new family of ras genes, the rho genes, which share several properties with the more classical ras gene family consisting of n-, k-, and h-ras. the rho genes, first isolated from a cdna library from the abdominal ganglia of aplysia, encode proteins that share 35% amino acid homology with h-ras. evolutionarily conserved counterparts of rho have been detected in yeast, in drosophila, in rat, and in man. sequence analysis reveals over 85% homology between the human and aplysia ... | 1985 | 3888408 |
| differential activation of yeast adenylate cyclase by wild-type and mutant ras proteins. | in these experiments we demonstrate that purified ras proteins, whether derived from the yeast ras1 or ras2 or the human h-ras genes, activate yeast adenylate cyclase in the presence of guanine nucleotides. these results confirm the prediction of earlier genetic and biochemical data and for the first time provide a complete biochemical assay for ras protein function. furthermore, we observe a biochemical difference between the ras2 and ras2val19 proteins in their ability to activate adenylate cy ... | 1985 | 3891097 |
| studies of human natural killer cytotoxic factor (nkcf): characterization and analysis of its mode of action. | soluble natural killer cytotoxic factors (nkcf) have been detected in the supernatant of normal mouse, rat, and human lymphocytes stimulated in vitro for 1 to 3 days in serum-free medium. stimulation of large granular lymphocytes (lgl) with nk-sensitive targets or mitogens has resulted in high levels of nkcf production. previous studies in the mouse and human systems have analyzed the cells responsible for production, specificity, and general characteristics of nkcf. in the present study, using ... | 1985 | 3898750 |
| photochemotherapy using pyridopsoralens. | aiming to decrease the acute side effects and genotoxic hazards of puva, pyrido (3,4-c) psoralen (pp) and 7-methyl pyrido (3,4-c) psoralen (mpp) were synthesized and studied. their uva maximum absorption lies at 325 and 330 nm, respectively. their photostability is comparable to that of 8-mop. they complex to dna in the dark, and, in the presence of uva, produce only monoadditions to dna, as shown by fluorescence and dna denaturation-renaturation studies. in diploid eukaryotic yeast they are mor ... | 1985 | 3899194 |
| factors governing adherence of candida species to plastic surfaces. | the ability of candida albicans and candida spp. to adhere to inert polymeric surfaces may allow these organisms direct ingress into the human host. biophysical characterization of this adherence shows that the forces responsible for such adherence are attractive london-van der waals forces (or hydrophobic forces) and electrostatic forces. the hydrophobic affinity of yeasts was determined by (i) a water-hydrocarbon two-phase assay and by (ii) measurement of the contact angle (theta) of a liquid ... | 1985 | 3899942 |
| molecular cloning and regulated expression of the human c-myc gene in escherichia coli and saccharomyces cerevisiae: comparison of the protein products. | mrna from human hl-60 cells was used to prepare a cdna library, from which two full-length clones that encompass the complete c-myc coding region were isolated. one clone, pm1-11, contains all three exons of human c-myc. the second clone, pm4-10, represents a relatively rare transcript that initiated in the first intron and includes the coding exons 2 and 3. the cdna insert in pm1-11 was used to express the human c-myc protein in both prokaryotic and eukaryotic cells. insertion of the coding seq ... | 1985 | 3903749 |
| adherence of candida to cultured vascular endothelial cells: mechanisms of attachment and endothelial cell penetration. | to elucidate the pathogenesis of hematogenous candida infections, we developed an in vitro model of candida adherence to and penetration of human endothelial cells. we enhanced or inhibited adherence in order to probe mechanisms of attachment. adherence of candida albicans showed a linear relation to candida inoculum (range, 10(2)-10(5) cfu, r = .99, p less than .01) and exceeded that of less virulent candida species and that of saccharomyces cerevisiae (p less than .01). candida immune serum bl ... | 1985 | 3905987 |
| expression of a synthetic human growth hormone gene in yeast. | a synthetic human growth hormone (hgh) gene was efficiently expressed under the control of the repressible acid phosphatase promoter in yeast (saccharomyces cerevisiae). more than 10(6) molecules of hormone were formed per cell despite the fact that the gene was constructed with codon preference for escherichia coli. | 1985 | 3908222 |
| production of human c-myc protein in insect cells infected with a baculovirus expression vector. | a cdna fragment coding for human c-myc was inserted into the genome of the baculovirus autographa californica nuclear polyhedrosis virus adjacent to the strong polyhedrin promoter. insect cells infected with the recombinant virus produced significant amounts of c-myc protein, which constituted the major phosphoprotein component in these cells. by immunoprecipitation and immunoblot analysis, two proteins of 61 and 64 kilodaltons were detected with c-myc-specific antisera. the insect-derived prote ... | 1985 | 3915537 |
| characterization and developmental expression of a drosophila ras oncogene. | we cloned a drosophila melanogaster ras gene (dmras64b) on the basis of its homology to the ras oncogen from harvey murine sarcoma virus. this gene mapped at chromosomal position 64b on the left arm of the third chromosome. sequencing of dmras64b revealed extensive amino acid homology with the proteins encoded by the human and saccharomyces cerevisiae ras genes. the coding region of the drosophila gene is interrupted by two introns located in different positions with respect to its human counter ... | 1985 | 3921827 |
| limiting role of 6-phosphogluconolactonase in erythrocyte hexose monophosphate pathway metabolism. | the natural product of the glucose-6-phosphate dehydrogenase reaction is 6-phosphoglucono-delta-lactone, which must be hydrolyzed to 6-phosphogluconic acid before it can be further metabolized by 6-phosphogluconate dehydrogenase. because this lactone is very unstable, it has been uncertain whether the enzyme that hydrolyzes it, 6-phosphogluconolactonase, is required for functioning of the hexose monophosphate pathway. we have purified glucose-6-phosphate dehydrogenase, 6-phosphogluconolactonase, ... | 1985 | 3932573 |
| expression of active human uterine tissue plasminogen activator in yeast. | tissue-type plasminogen activator (tpa) cdna derived from human uterine mrna was inserted into different yeast expression vectors. all such expression plasmids carried a yeast acid phosphatase (pho5) promoter, a 2-micron plasmid replication origin, transcription termination signals, and a selectable trp1 gene. plasmid pybdt-10 contained the entire tpa coding region ("pre-pro-tpa"), pybdt-10-pro contained a sequence encoding the putative pro-tpa precusor, and pybdt-6 contained only a mature tpa c ... | 1985 | 3937715 |
| expression of normal and activated human ha-ras cdnas in saccharomyces cerevisiae. | we expressed normal and activated human cellular ha-ras cdnas which encode 21,000-dalton polypeptides (p21s) in saccharomyces cerevisiae by their insertion into a 2 micron-based replicating plasmid vector under 3-phosphoglycerate kinase promoter control. we found that newly synthesized p21 in s. cerevisiae was produced as a soluble precursor (pro-p21) which matured into a form electrophoretically indistinguishable from the processed form (p21) observed in mammalian cells. coincident with the pro ... | 1985 | 3939254 |
| synthesis of small nuclear ribonucleoprotein particles by the malarial parasite plasmodium falciparum. | sera from patients with autoimmune diseases have been used to identify small nuclear ribonucleoprotein particles (snrnps) present in higher eukaryotic cells and also in dinoflagellates. previously these sera have not detected crossreactive snrnp protein antigens of other lower eukaryotes such as yeast, tetrahymena, or dictyostelium. we report that anti-sm, anti-u1-rnp, and anti-la/ss-b human antisera react with specific snrnp protein antigens synthesized by the protozoan plasmodium falciparum, t ... | 1985 | 2582421 |
| deposition of c3b and ic3b onto particulate activators of the human complement system. quantitation with monoclonal antibodies to human c3. | monoclonal antibodies were used to determine the number and molecular form of c3 bound to particulate activators of the complement (c) system by human serum. sheep erythrocytes (e) coated with igm (eigm) and igg (eigg) were used to study activation of the classical pathway (cp). yeast (y), rabbit erythrocytes (er), and five species of bacteria (escherichia coli, staphylococcus aureus, streptococcus pneumoniae type 3, streptococcus pyogenes, and hemophilus influenzae type b) were used to study ac ... | 1985 | 2409200 |
| amino acid sequence of the alpha subunit of transducin deduced from the cdna sequence. | transducin, a gtp-binding protein involved in phototransduction in the vertebrate retina, belongs to a family of homologous coupling proteins that also includes gs and gi, the regulatory proteins of adenylate cyclase. here we report the cdna sequence and deduced amino acid sequence of transducin's alpha subunit (t alpha). the cdna was isolated, by screening with an antibody probe, from a bovine retinal cdna library in the expression vector lambda gt11. the 2.2-kilobase cdna insert hybridized to ... | 1985 | 2409555 |
| virion proteins and the perspectives of gene manipulations in vaccine preparation. | the achievements and perspectives of genetic manipulations are described aiming at preparation of first generation subunit vaccines based on the synthesis in bacterial and eukaryotic cells of full-sized virion proteins expressing the main antigenic determinants. the preparation of such vaccines in bacterial cells seems out of perspective in the case of influenza, human hepatitis b, foot- and - mouth disease and some other viruses due to the peculiarities of structure and synthesis as well as low ... | 1985 | 2413750 |
| characterization of an rna polymerase activity from hela cell mitochondria, which initiates transcription at the heavy strand rrna promoter and the light strand promoter in human mitochondrial dna. | an rna polymerase activity capable of initiating transcription at both the heavy strand rrna promoter and the light strand promoter of human mitochondrial dna has been partially purified from hela cell mitochondria and characterized in its requirements and products. the ratio of the two transcription initiating activities varied considerably from preparation to preparation. the human mtrna polymerase partially purified by deae-cellulose and heparin-agarose chromatography exhibits a great sensiti ... | 1985 | 2981880 |
| [viral hepatitis and immunoprophylaxis]. | today we distinguish 4 forms of viral hepatitis: hepatitis a, hepatitis b, hepatitis non-a, non-b and hepatitis occurring in the course of other viral diseases. the viruses of hepatitis a and hepatitis b have been identified but the agent(s) of hepatitis non-a, non-b remain unknown. inoculation of normal pooled human immunoglobulin provides passive immunity for 2-3 months against hepatitis a but not against hepatitis b or hepatitis non-a, non-b. for passive protection against hepatitis b a speci ... | 1985 | 2986380 |
| human spleen dihydroorotate dehydrogenase: a study of inhibition of the enzyme. | seventy-one pyrimidine analogs have been tested as possible inhibitors of human spleen mitochondrial dihydroorotate dehydrogenase. of these nine were demonstrated to be effective inhibitors of the enzymic activity. two compounds, dihydro-5-azaorotate and 6-thiobarbiturate appeared to be specific inhibitors of the dho-dhase. in addition, three compounds, 5-azaorotate, 5-bromoorotate, and barbiturate were also inhibitory against the two subsequent enzymes of the pathway, orotate phosphoribosyltran ... | 1985 | 2996510 |
| cytochemical study of macrophage lysosomal inorganic trimetaphosphatase and acid phosphatase. | cytochemical investigations have associated acid inorganic trimetaphosphatase (tmpase) activity with the lysosomes of certain cell types. we have used the modified staining technique of berg to show that this enzyme activity is present in normal mononuclear phagocytes and macrophage cell lines. we have found this enzyme activity to be present in murine raw264 macrophages, in human u937 macrophages, in normal human blood monocytes, and in guinea pig peritoneal macrophages. all of the raw264 and u ... | 1985 | 2999416 |
| viral hepatitis: implications to pediatric practice. | a major frontier is the development of effective therapy for patients with chronic type b hepatitis, which currently remains refractory to all known antiviral agents and is associated with severe long-term consequences. future research should focus on the relationship of hbv to hepatocellular carcinogenesis. this might answer the question as to when integration of viral dna into the host cell occurs and whether this is an irreversible step in the progression toward hepatocellular carcinoma. the ... | 1985 | 3002154 |
| the b chain of pdgf alone is sufficient for mitogenesis. | the platelet-derived growth factor (pdgf) is a mitogen derived from human platelets consisting of two related polypeptides termed a and b chains. the entire b chain of pdgf is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus. it has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of pdgf and stimulating the cell in an autocrine manner. we have directly examined the mitogenic potential of p28si ... | 1985 | 3004957 |
| alphaherpesviruses possess a gene homologous to the protein kinase gene family of eukaryotes and retroviruses. | the us3 genes of herpes simplex virus serotypes 1 and 2, and the corresponding gene of varicella-zoster virus, encode proteins whose sequences are clearly homologous to members of the protein kinase family of eukaryotes and retroviruses. similarity is most characteristic, and strongest, in an 80 residue region comprising part of the catalytic structure of the kinases. in this region the herpesvirus proteins are most like a yeast cell division control protein, and least like the retrovirus protei ... | 1986 | 3005981 |
| cloning, sequencing, and species relatedness of the escherichia coli cca gene encoding the enzyme trna nucleotidyltransferase. | the escherichia coli cca gene which encodes the enzyme trna nucleotidyltransferase has been cloned by taking advantage of its proximity to the previously cloned dnag locus. a series of recombinant bacteriophages, spanning the chromosomal region between the dnag and cca genes at 66 min on the e. coli linkage map, were isolated from a lambda charon 28 partial sau3a e. coli dna library using recombinant plasmids containing regions between dnag and cca as probes. two of the recombinant phage isolate ... | 1986 | 3009457 |
| in vitro immunization for the production of antigen-specific lymphocyte hybridomas. | 1986 | 3014268 | |
| a possible competition between 5'-monodeiodination of thyroxine and the respiratory burst in human granulocytes. | thyroxine (t4) pretreatment of a 23187-stimulated human granulocytes in 10(-5)-10(-6) m concentration range inhibited the superoxide anion production of these cells. t4 increased the level of oxidized form of glutathione, whereas the intracellular level of the reduced form decreased. a similar alteration in the ratio of the oxidized to reduced forms of glutathione was detected in granulocytes during yeast cell phagocytosis. in addition, conversion of t4 to triiodothyronine (t3) was also inhibite ... | 1986 | 3015757 |
| mechanism of interaction between ku protein and dna. | the mechanism of interaction between the ku autoantigenic protein, a heterodimer of noncovalently linked 70,000- and 80,000-dalton subunits, and dna was studied using immunoaffinity-purified ku protein and a 300-base pair ecori fragment from hela cell dna. in the nitrocellulose filter-binding assay, the ku protein bound 32p-labeled double-stranded dna, and much less efficiently single-stranded dna. the binding of ku to dna was dependent on ionic strength and prevented by igg from patient sera co ... | 1986 | 3015926 |
| part of the human ribosomal rna locus stabilizes a plasmid in yeast. | most yeast plasmids--particularly those containing chromosomal replicators (ars)--are unstable and do not segregate equally to mother and daughter cells unless they contain centromeric sequences. we have screened a fraction of the human genome for sequences that stabilize yrp7, a plasmid containing ars1. we selected a fraction which we hoped would be enriched in human centromeric sequences--the dna attached to the nucleoskeleton. we obtained one human sequence that partially stabilized a yeast p ... | 1986 | 3016661 |
| construction of expression vectors for secretion of human interferons by yeast. | 1986 | 3020352 | |
| recombinant human granulocyte-macrophage colony-stimulating factor stimulates in vitro mature human neutrophil and eosinophil function, surface receptor expression, and survival. | a purified recombinant human granulocyte-macrophage colony stimulating factor (rh gm-csf) was a powerful stimulator of mature human eosinophils and neutrophils. the purified rh gm-csf enhanced the cytotoxic activity of neutrophils and eosinophils against antibody-coated targets, stimulated phagocytosis of serum-opsonized yeast by both cell types in a dose-dependent manner, and stimulated neutrophil-mediated iodination in the presence of zymosan. in addition, rh gm-csf enhanced n-formylmethionyll ... | 1986 | 3021817 |
| the ral gene: a new ras related gene isolated by the use of a synthetic probe. | we synthesized a set of 20-mer oligonucleotides corresponding to a sequence of seven amino acids strictly conserved in all the different ras proteins, from yeast to man, as well as in rho and ypt, two proteins distantly related to p21 ras (approximately 30% amino acid homology). this oligonucleotide probe was used to search for new members of the ras family. we describe here a new ras related gene named ral, isolated from a cdna library of immortalized simian b-lymphocytes. the ral gene codes fo ... | 1986 | 3023062 |
| saccharomyces cerevisiae secretes and correctly processes human interferon hybrid proteins containing yeast invertase signal peptides. | synthetic oligonucleotides coding for the yeast invertase secretion signal peptide were fused to the gene for the mature form of human interferon (huifn-alpha 2). two plasmids (e3 and f2) were constructed. e3 contained the invertase signal codons in a reading frame with the mature huifn-alpha 2 gene. f2 had a deletion of the codon for alanine at amino acid residue-5 in the invertase signal and an addition of a methionine codon located between the coding sequences for the invertase signal and mat ... | 1986 | 3023906 |
| xrep, a plasmid-stimulating x chromosomal sequence bearing similarities to the bk virus replication origin and viral enhancers. | the human x chromosome-linked fragment, "xrep," was sequenced because it exerts a positive effect on plasmid growth in both e. coli and saccharomyces cerevisiae. the sequence revealed three features similar to the human bk virus replication origin: xrep has a true palindrome, cctcc(t)3cctcc, which is similar to "true" palindrome-like sequences found at the replication origins of polyoma [cctc(t/c)10ctcc], bk [cctc(a/g)8cctcc] and sv40 [cctcc(a)6gcctcc] viruses. twenty nucleotides away from the t ... | 1986 | 3025813 |
| htlv-iii gag protein is processed in yeast cells by the virus pol-protease. | the gag-pol gene of htlv-iii (human t-lymphotropic virus), the virus linked to aids (acquired immune deficiency syndrome), was expressed in yeast, and processing of the gag precursor into proteins of the same size as those in the virion was observed. processing of the gag gene in yeast cells mimics the process that naturally occurs in mammalian cells during maturation of virions. therefore it was possible to perform mutational analysis of the virus genome to localize the gene that codes for the ... | 1986 | 2420008 |
| expression of hepatitis b virus surface antigen p31 gene in yeast. | the hepatitis b virus surface antigen (hbsag) p31 gene has been expressed in yeast saccharomyces cerevisiae. the gene products were shown to be glycoproteins with molecular sizes of 37,000 and 34,000 daltons (gp37 and gp34) containing polymerized albumin receptors. successfully detecting these proteins depended on the extraction procedures. in the extract without protein denaturants and inhibitors, these products were degraded rapidly by proteases to yield smaller size derivatives lacking polyme ... | 1986 | 2427075 |
| a brief overview of the new vaccines against hepatitis b virus infection: immunogenic gene products and peptide analogues of antigenic epitopes. | immunologically recognizable antigens encoded by hepatitis b virus (hbv) dna include: (i) the surface antigen (hbsag), (ii) the pre-s antigen, (iii) the x antigen (hbxag) and (iv) the core/e antigens (hbcag/hbeag). each one of these antigens or their combination may be prepared for the next generation of vaccines against hbv infection. the synthesis of hbsag by expression of recombinant dna in the yeast system has now reached the stage of clinical trials and will certainly provide the first alte ... | 1986 | 2427376 |
| production and immunological analysis of recombinant hepatitis b vaccine. | the synthesis of the hepatitis b surface antigen (hbsag) in cells of saccharomyces cerevisiae and its subsequent isolation, purification and analysis is described. the final, purified hbsag particle exhibits close structural and biochemical similarities to particles derived from the plasma of chronically infected humans. particles of yeast and human origin have been found, by chimpanzee efficacy studies and by various in vitro analyses, to be immunologically equivalent. the antigenic expression ... | 1986 | 2427590 |
| novel hepatitis b vaccines. | development of vaccines against hepatitis b has proceeded along four main lines. human plasma-derived vaccines are safe, effective, and in general use. subunit polypeptide vaccines formulated in micelles have reached the stage of clinical trials. recombinant dna vaccines have been produced in prokaryotic and eukaryotic cells, notably in yeast. the yeast-derived recombinant vaccine has proved safe and effective in extensive clinical trials, eliciting antibodies of equal quantity and quality of sp ... | 1986 | 2427591 |
| [structure and expression of human hepatitis b virus surface antigen]. | in view of the growing occurrence rate of the virus-induced hepatitis b and also of the special role played by this particular virus (hbv) in the application of recombinant genetic techniques to the study of complex biological systems, an attempt was made to survey the available evidence concerning the widely investigated and practically the most important part of the viral genome, viz. the gene coding for the surface antigen (hbsag) and the protein itself. the possible antigenic structure of th ... | 1986 | 2429171 |
| biochemical and cellular determinants of bleomycin cytotoxicity. | bleomycin is a mixture of cytotoxic glycopeptides which function as mininucleases, binding to dna and producing single and double strand breaks by the formation of an activated oxygen complex. bleomycin is an effective agent against a few human cancers, notably lymphomas, testicular and ovarian germ cell cancers and certain squamous carcinomas. most human cancers are resistant to bleomycin a priori, however, and those which are initially sensitive frequently develop resistance to the drug during ... | 1986 | 2439200 |
| immunological cross-reactivity of alcohol dehydrogenase (adh) isozymes with rabbit immune sera against horse and human adh subunits. | rabbit immune sera raised against denatured forms of horse liver alcohol dehydrogenase and of human adh5 isozyme were found to react with the denatured subunits of all the human adh isozymes regardless of their class. the immune serum against the human adh isozyme cross-reacted also with horse adh subunits and, at appropriate dilutions, both the immune sera reacted with denatured yeast adh, suggesting that common structures have been preserved in these molecules over a long evolutionary period. ... | 1986 | 2451467 |
| unscheduled dna synthesis in xeroderma pigmentosum cells after microinjection of yeast photoreactivating enzyme. | photoreactivating enzyme (pre) from yeast causes a light-dependent reduction of uv-induced unscheduled dna synthesis (uds) when injected into the cytoplasm of repair-proficient human fibroblasts (zwetsloot et al., 1985). this result indicates that the exogenous pre monomerizes uv-induced dimers in these cells competing with the endogenous excision repair. in this paper we present the results of the injection of yeast pre on (residual) uds in fibroblasts from different excision-deficient xp-strai ... | 1986 | 3951462 |
| some observations made by intracellular recordings in a primary astrocyte culture and a glioblastoma '138 mg'. | intracellular recordings were performed in a primary astrocyte culture from rat brain and in a human glioblastoma cell line, 138 mg. the technique proved insufficient to verify the heterogeneous composition of the primary astrocyte culture, since this study shows most cells present in the culture to have similar resting membrane potential, membrane impedance, membrane potential/impedance relationship and k+-sensitivity. with the exception of macrophages, identified by their response to externall ... | 1986 | 3962687 |
| aminopeptidase iii activity in normal and cataractous lenses. | aminopeptidase iii activity was demonstrated in extracts from several different mammalian lenses by the hydrolysis of arg-mca at ph 6.0. no more than a two-fold difference was seen in overall specific activity. sections of bovine lenses were removed from the periphery to the center and assayed. a sharp decline in activity was observed in the inner cortical region, and little or no activity was observed in the lens nucleus. this correlated with an increase in the presence of low molecular weight ... | 1986 | 3720344 |
| chromosomal protein hmg-17. complete human cdna sequence and evidence for a multigene family. | antibodies elicited against chromosomal protein hmg-17, purified from calf, were used to screen a human lambda gt11 cdna expression library and isolate the full length cdna coding for this protein. sequence analysis reveals that the nucleotide distribution along this cdna is highly asymmetric. the amino acid sequence, deduced from the reading frame, reveals that the human hmg-17 is, respectively, 96 and 92% homologous with the calf and chicken protein. the amino acid substitution are conservativ ... | 1986 | 3754870 |
| crystallographic characterization of recombinant human cuzn superoxide dismutase. | recombinant human cuzn superoxide dismutase as expressed in yeast has been crystallized in three different crystal forms. hexagonal plates grow from 2.4 m ammonium sulfate, ph 7.5, and belong to the space group p6(3)22, with cell dimensions a = b = 113.5(3), c = 151.5(5) a, and vm = 2.21 a3/dalton for two dimers per asymmetric unit. at 2.0 m ammonium sulfate, ph 7.5, chunky wedges grow in space group c222(1), a = 205.2(6), b = 166.5(4), c = 145.4(4) a with a vm of 2.43 a3/dalton for eight dimers ... | 1986 | 3782115 |
| primary structure of human ribosomal protein s14 and the gene that encodes it. | chinese hamster ribosomal protein s14 cdna was used to recognize homologous human cdna and genomic clones. human and chinese hamster s14 protein sequences deduced from the cdnas are identical. two overlapping human genomic s14 dna clones were isolated from a charon 28 placental dna library. a fragment of single-copy dna derived from an intron region of one clone was mapped to the functional rps14 locus on human chromosome 5q by using a panel of human x chinese hamster hybrid cell dnas. the human ... | 1986 | 3785212 |
| expression of the hepatitis b virus large envelope protein in saccharomyces cerevisiae. | the yeast vector ppv2 has been constructed for inducible expression of non-fused proteins from the pho5 promoter. signals of the ura3 gene are used for transcription termination. the 226-amino-acid 'major' and the 389-amino-acid 'large' envelope protein of hepatitis b virus (hbv) have been produced in saccharomyces cerevisiae following insertion of the s gene or of the entire pre-s region and the s gene, respectively, of hbv into ppv2. although normally only a minor constituent of the viral enve ... | 1986 | 3549460 |
| expression of the human salivary alpha-amylase gene in yeast and characterization of the secreted protein. | recombinant plasmids were constructed in which the human salivary alpha-amylase gene, with or without the n-terminal signal sequence for secretion, was placed under control of the apase (pho5) promoter of saccharomyces cerevisiae. in yeast cells transformed with the alpha-amylase gene having the human signal sequence for secretion, the gene was expressed and the enzyme was secreted into the medium in three different glycosylated forms. the amylase gene without the signal sequence was also expres ... | 1986 | 3556325 |
| a processed chicken pseudogene (cps1) related to the ras oncogene superfamily. | we describe the first polya-containing processed pseudogene reported in the chicken. it includes a 0.52 kb open reading frame which could encode a 175 amino acid protein. the putative protein shows extensive homology to the ras oncogene superfamily, being most closely related to the yeast protein yp2. it is one of the two most divergent members of the ras superfamily yet described and is the most homologous of any ras-related protein to the g-protein alpha-transducin. the chicken genome contains ... | 1986 | 3083400 |
| abnormal response to dna crosslinking agents of fanconi anemia fibroblasts can be corrected by transfection with normal human dna. | primary skin fibroblast cell lines from patients with fanconi anemia were cotransfected with uv-irradiated psv2neo plasmids and high molecular weight dna from normal human cells. restoration of a normal cellular resistance to mitomycin c (mmc) was observed provided that a fanconi anemia cell line is selected for dna-mediated transformation (neo gene) and that at least two successive rounds of transfection are performed. cells were selected by taking advantage of the higher proliferation rate and ... | 1986 | 3092225 |
| potent antagonism of escherichia coli, bacteroides ovatus, fusobacterium varium, and enterococcus faecalis, alone or in combination, for enteropathogens in anaerobic continuous flow cultures. | interactions between representative strains of four predominant resident bacteria of the human colon, escherichia coli, enterococcus faecalis, bacteroides ovatus, and fusobacterium varium, and strains of seven enteropathogens, yersinia enterocolitica, shigella flexneri, salmonella typhimurium, vibrio parahaemolyticus, v. cholerae serogroup non o1, staphylococcus aureus, and clostridium perfringens, were examined in studies with an anaerobic continuous flow culture system and medium resembling th ... | 1986 | 2875188 |
| uncoating atpase is a member of the 70 kilodalton family of stress proteins. | the synthetic peptide, vgidlgttysc, derived from the heat shock-induced genes human hsp70, drosophila hsp70, s. cerevisiae yg100, and e. coli dnak, elicited antibodies that recognized two constitutive proteins in bovine extracts. one of these proteins, 71 kd, has previously been identified as uncoating atpase, an enzyme that releases clathrin from coated vesicles. this immunological data complemented the result that uncoating atpase was indistinguishable from the constitutive mammalian 71 kd str ... | 1986 | 2937542 |
| evidence for the intercalation of thalidomide into dna: clue to the molecular mechanism of thalidomide teratogenicity? | the intercalation of thalidomide (alpha-phthalimidoglutarimide) into nucleic acids was studied by spectrophotometric titrations, displacement of known intercalators, viscosity changes of dna solutions, and by phase partition analysis. specific binding of thalidomide was found to dna of various specimens (human placenta, calf thymus, salmon sperm), but not to rna (from bakers yeast, torula yeast). | 1986 | 2953123 |
| molecular cloning of cdna for the nuclear ribonucleoprotein particle c proteins: a conserved gene family. | the c proteins, c1 and c2, are major constituents of the heterogeneous nuclear rna (hnrna) ribonucleoprotein (hnrnp) complex in vertebrates. c1 and c2 are antigenically related phosphoproteins that are in contact with hnrna in intact cells and bind to rna tightly in vitro. a cdna clone for the c proteins was isolated by immunological screening of a human lambda gt11 expression vector cdna library with monoclonal antibodies. the lacz-cdna fusion protein is recognized by two different anti-c prote ... | 1986 | 3457372 |
| yeast organisms associated with human oral leukoplakia. | oral leukoplakia is a lesion of variable clinical behaviour and a malignant transformation rate of 3-6%. leukoplakia exhibits various histologic features, hyperkeratinization being the most consistent. as yeast organisms have been encountered in a high proportion of leukoplakia cases, a causal role has been suggested for these organisms. candida albicans is by far the most commonly isolated yeast from leukoplakia, but also other candida species as well as torulopsis and saccharomyces species may ... | 1986 | 3459346 |
| cloning and sequence analysis of cdna for human argininosuccinate lyase. | using antibodies specific for argininosuccinate lyase (ec 4.3.2.1), we isolated two cdna clones by screening a human liver cdna library constructed in the lambda gt11 expression vector. the identity of these isolates was confirmed by in vitro translation of plasmid-selected mrna. one of these isolates was used to rescreen the cdna library and a 1565-base-pair (bp) clone was identified. the entire nucleotide sequence of this clone was determined. an open reading frame was identified which encoded ... | 1986 | 3463959 |
| the primary structure of the alpha subunit of human elongation factor 1. structural aspects of guanine-nucleotide-binding sites. | the primary structure of the alpha subunit of elongation factor 1 (ef-1 alpha) from human molt 4 cells was determined by cdna sequencing. the data show that the conservation of the amino acid sequence is more than 80% when compared with yeast and artemia ef-1 alpha. an inventory of amino acid sequences around the guanine-nucleotide-binding site in elongation factor tu from escherichia coli and homologous amino acid sequences in g proteins, initiation and elongation factors and proteins from the ... | 1986 | 3512269 |
| a sensitive and rapid gel retention assay for nuclear factor i and other dna-binding proteins in crude nuclear extracts. | the paper describes a rapid and sensitive assay for dna binding proteins which interact with specific and defined binding sites. it exploits the observation that complexes of proteins and small synthetic dna fragments (40 bp) containing the protein/dna binding site can enter native polyacrylamide gels and remain stably associated during electrophoresis under non-denaturing conditions. the assay was applied to nuclear factor i, to its identification and purification from porcine liver, to an anal ... | 1986 | 3513122 |
| activation of the complement system by cryptococcus neoformans leads to binding of ic3b to the yeast. | the complement system plays a key role in resistance to cryptococcosis. in the present study, we examined several factors that influence the binding of c3 cleavage fragments to cryptococcus neoformans. binding of c3 was determined by using normal human serum supplemented with 125i-labeled c3. incubation of encapsulated cryptococci in 20% serum led to the binding of approximately 3.2 x 10(6) molecules of c3 to each cell. the binding of c3 was markedly inhibited by heating the serum at 56 degrees ... | 1986 | 3514450 |