Publications

determination the usefulness of ahhma4p1::ahhma4 expression in biofortification strategies.	ahhma4 from arabidopsis thaliana encodes zn/cd export protein that controls zn/cd translocation to shoots. the focus of this manuscript is the evaluation of ahhma4 expression in tomato for mineral biofortification (more zn and less cd in shoots and fruits). hydroponic and soil-based experiments were performed. transgenic and wild-type plants were grown on two dilution levels of knop's medium (1/10, 1/2) with or without cd, to determine if mineral composition affects the pattern of root/shoot par ...	2017	27358503
root microbiota dynamics of perennial arabis alpina are dependent on soil residence time but independent of flowering time.	recent field and laboratory experiments with perennial boechera stricta and annual arabidopsis thaliana suggest that the root microbiota influences flowering time. here we examined in long-term time-course experiments the bacterial root microbiota of the arctic-alpine perennial arabis alpina in natural and controlled environments by 16s rrna gene profiling. we identified soil type and residence time of plants in soil as major determinants explaining up to 15% of root microbiota variation, wherea ...	2017	27482927
gene-regulatory networks controlling inflorescence and flower development in arabidopsis thaliana.	reproductive development in plants is controlled by complex and intricate gene-regulatory networks of transcription factors. these networks integrate the information from endogenous, hormonal and environmental regulatory pathways. many of the key players have been identified in arabidopsis and other flowering plant species, and their interactions and molecular modes of action are being elucidated. an emerging theme is that there is extensive crosstalk between different pathways, which can be acc ...	2017	27487457
photochemical activity and the structure of chloroplasts in arabidopsis thaliana l. mutants deficient in phytochrome a and b.	the reduced content of photoreceptors, such as phytochromes, can decrease the efficiency of photosynthesis and activity of the photosystem ii (psii). for the confirmation of this hypothesis, the effect of deficiency in both phytochromes (phy) a and b (double mutant, dm) in 7-27-day-old arabidopsis thaliana plants on the photosynthetic activity was studied in absence and presence of uv-a radiation as a stress factor. the dm with reduced content of apoproteins of phya and phyb and wild type (wt) p ...	2017	27586644
the rnas of rna-directed dna methylation.	rna-directed chromatin modification that includes cytosine methylation silences transposable elements in both plants and mammals, contributing to genome defense and stability. in arabidopsis thaliana, most rna-directed dna methylation (rddm) is guided by small rnas derived from double-stranded precursors synthesized at cytosine-methylated loci by nuclear multisubunit rna polymerase iv (pol iv), in close partnership with the rna-dependent rna polymerase, rdr2. these small rnas help keep transposo ...	2017	27521981
glycosylation of arabinogalactan-proteins essential for development in arabidopsis.	arabinogalactan-proteins (agps) are ubiquitous cell wall components present throughout the plant kingdom. they are extensively post translationally modified by conversion of proline to hydroxyproline (hyp) and by addition of arabinogalactan (ag) polysaccharides to hyp residues. two small gene subfamilies within the cazy gt31 family, referred to as hyp-galactosyltransferases (hyp-galts and hpgts), encode enzymes that specifically add galactose to agp protein backbones as revealed by heterologous ...	2017	27489583
effects of elevated carbon dioxide on stomatal characteristics and carbon isotope ratio of arabidopsis thaliana ecotypes originating from an altitudinal gradient.	stomatal functioning regulates the fluxes of co2 and water vapor between vegetation and atmosphere and thereby influences plant adaptation to their habitats. stomatal traits are controlled by external environmental and internal cellular signaling. the objective of this study was to quantify the effects of co2 enrichment (ce) on stomatal density (sd)-related properties, guard cell length (gcl) and carbon isotope ratio (δ(13) c) of a range of arabidopsis thaliana ecotypes originating from a wide a ...	2017	27514017
the class iii peroxidase prx17 is a direct target of the mads-box transcription factor agamous-like15 (agl15) and participates in lignified tissue formation.	several physiological functions have been attributed to class iii peroxidases (prxs) in plants, but the in planta role of most members of this family still remains undetermined. here, we report the first functional characterization of prx17 (at2g22420), one of the 73 members of this family in arabidopsis thaliana. localization of prx17 was examined by transient expression in nicotiana benthamiana. loss- and gain-of-function mutants in a. thaliana were studied. regulation at the gene and protein ...	2017	27513887
amap: a pipeline for whole-genome mutation detection in arabidopsis thaliana.	detection of mutations at the whole-genome level is now possible by the use of high-throughput sequencing. however, determining mutations is a time-consuming process due to the number of false positives provided by mutation-detecting programs. amap (automated mutation analysis pipeline) was developed to overcome this issue. amap integrates a set of well-validated programs for mapping (bwa), removal of potential pcr duplicates (picard), realignment (gatk) and detection of mutations (samtools, gat ...	2017	27452041
reduced paucimannosidic n-glycan formation by suppression of a specific β-hexosaminidase from nicotiana benthamiana.	plants are attractive hosts for the production of recombinant glycoproteins for therapeutic use. recent advances in glyco-engineering facilitate the elimination of nonmammalian-type glycosylation and introduction of missing pathways for customized n-glycan formation. however, some therapeutically relevant recombinant glycoproteins exhibit unwanted truncated (paucimannosidic) n-glycans that lack glcnac residues at the nonreducing terminal end. these paucimannosidic n-glycans increase product hete ...	2017	27421111
nucleotide sequence of phaseolus vulgaris l. alcohol dehydrogenase encoding cdna and three-dimensional structure prediction of the deduced protein.	common beans (phaseolus vulgaris l.) are widely consumed as a source of proteins and natural products. however, its yield needs to be increased. in line with the agenda of phaseomics (an international consortium), work of expressed sequence tags (ests) generation from bean pods was initiated. altogether, 5972 ests have been isolated. alcohol dehydrogenase (ad) encoding gene cdna was a noticeable transcript among the generated ests. this ad is an important enzyme; therefore, to understand more ab ...	2017	25829796
how does photoreceptor uvr8 perceive a uv-b signal?	uvr8 is the only known plant photoreceptor that mediates light responses to uv-b (280-315 nm) of the solar spectrum. uvr8 perceives a uv-b signal via light-induced dimer dissociation, which triggers a wide range of cellular responses involved in photomorphogenesis and photoprotection. two recent crystal structures of arabidopsis thaliana uvr8 (atuvr8) have revealed unusual clustering of uv-b-absorbing trp pigments at the dimer interface and provided a structural framework for further mechanistic ...	2017	25996910
dynamic crystallography reveals early signalling events in ultraviolet photoreceptor uvr8.	arabidopsis thaliana uvr8 (atuvr8) is a long-sought-after photoreceptor that undergoes dimer dissociation in response to uv-b light. crystallographic and mutational studies have identified two crucial tryptophan residues for uv-b responses in atuvr8. however, the mechanism of uv-b perception and structural events leading up to dimer dissociation remain elusive at the molecular level. we applied dynamic crystallography to capture light-induced structural events in photoactive atuvr8 crystals. her ...	2017	26097745
[the expression profile of arabidopsis thaliana β-1,3-glucanase promoter in tobacco].	well characterized promoters with specific activity only during male gametophyte development of transgenic organism are widely utilized in strategies aimed at the elimination of unwanted transgene escape from the transgenic to the non-transgenic plant population. since the specificity and timing of the applied promoter in the original and transgenic organism don't have to be consistent, here we have tested by promoter-gus fusion analysis the aprs promoter isolated from arabidopsis thaliana in tr ...	2017	26299861
get the jump - do 3'utrs protect transposable elements from silencing?	eukaryotic genomes contain large numbers of transposable elements and repetitive sequences that are subjected to silencing through epigenetic mechanisms. these involve primarily dna methylation, chromatin modifications and small rna. it is known that these transposable elements can affect the expression of neighboring genes; however, little is known about how transposable element silencing depends on the general chromosomal environment at the insertion site. taking advantage of the vast genomic ...	2017	26442184
cell-type specific metabolic profiling of arabidopsis thaliana protoplasts as a tool for plant systems biology.	flow cytometry combined with cell sorting of protoplasts has previously been used successfully for transcript profiling of the arabidopsis thaliana root. we have developed the technique further, and in this paper we present a robust and reliable method for metabolite profiling in specific cell types isolated from arabidopsis roots. the method uses a combination of fluorescence-activated cell sorting and gas chromatography-time of flight-mass spectrometry analysis. cortical and endodermal cells f ...	2017	26491421
conversion of volatile alcohols into their glucosides in arabidopsis.	exposure of tomato plants to volatile chemicals emitted from common cutworm (spodoptera litura)-infested conspecifics led to accumulation of the glycoside, (z)-3-hexenyl vicianoside. accumulation of (z)-3-hexenyl vicianoside in the exposed plants has adverse impacts on the performance of the common cutworms. the aglycon of (z)-3-hexenyl vicianoside is derived from airborne (z)-3-hexenol emitted from infested plants. the ability to incorporate and convert (z)-3-hexenol to its corresponding glycos ...	2017	26629260
arabidopsis thaliana as a model organism in systems biology.	significant progress has been made in identification of genes and gene networks involved in key biological processes. yet, how these genes and networks are coordinated over increasing levels of biological complexity, from cells to tissues to organs, remains unclear. to address complex biological questions, biologists are increasingly using high-throughput tools and systems biology approaches to examine complex biological systems at a global scale. a system is a network of interacting and interde ...	2017	20228888
the heterotrimeric g-protein complex modulates light sensitivity in arabidopsis thaliana seed germination.	release of dormancy and induction of seed germination are complex traits finely regulated by hormonal signals and environmental cues such as temperature and light. the red (r):far-red (fr) phytochrome photoreceptors mediate light regulation of seed germination. we investigated the possible involvement of heterotrimeric g-protein complex in the phytochrome signaling pathways of arabidopsis thaliana seed germination. germination rates of null mutants of the alpha (galpha) and beta (gbeta) subunits ...	2017	19192205
comparative analysis of expressed sequence tag (est) libraries in the seagrass zostera marina subjected to temperature stress.	global warming is associated with increasing stress and mortality on temperate seagrass beds, in particular during periods of high sea surface temperatures during summer months, adding to existing anthropogenic impacts, such as eutrophication and habitat destruction. we compare several expressed sequence tag (est) in the ecologically important seagrass zostera marina (eelgrass) to elucidate the molecular genetic basis of adaptation to environmental extremes. we compared the tentative unigene (tu ...	2017	18239962