Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| neutralization of lymphocyte immortalization by different strains of epstein-barr virus with a murine monoclonal antibody. | a murine monoclonal antibody was raised against the b95-8 strain of epstein-barr virus (ebv), which was isolated from a case of mononucleosis after blood transfusion (hoffman et al. proc. natl. acad. sci. u.s.a. 77:2979, 1980). we provide evidence that neutralization of immortalization by this monoclonal antibody is virus specific, since its potency was inversely related to the dose of challenge virus. furthermore, the monoclonal antibody recognized antigens on viruses grown in human as well as ... | 1982 | 6182100 |
| immunochemical characterization of ebv antigens detected by double immunodiffusion. | double immunodiffusion (ouchterlony) tests gave regularly positive reactions when extracts of [35s]-methionine-labeled, n-butyrate-induced p3hr-1 cells were allowed to react with serum pools with high epstein-barr virus (ebv) antibody titers from burkitt lymphoma or nasopharyngeal carcinoma patients, but not with ebv antibody-negative sera. similarly prepared extracts of noninduced p3hr-1 cells gave no precipitation lines with the antibody-positive sera. the composition of the precipitates was a ... | 1982 | 6182123 |
| autoantibodies to nuclear antigens (ana): their immunobiology and medicine. | 1982 | 6182766 | |
| detection of antibodies to epstein-barr virus antigens by enzyme-linked immunosorbent assay. | an enzyme-linked immunosorbent assay (elisa) is described for the serologic analysis of antibodies to epstein-barr virus (ebv)-associated nuclear antigen (ebna), viral capsid antigen (vca), and early antigen (ea). the specificity of each of the elisas was demonstrated by the use of well-characterized human sera shown by immunofluorescence assay to be variously reactive for antibodies to one or more of the three viral antigens studied. the elisa for ebna was four to 256 times more sensitive than ... | 1982 | 6183369 |
| interaction of measles and epstein-barr viruses in transformed human lymphocytes. | 1982 | 6184957 | |
| differential expression and serologically distinct subpopulations of human ia antigens detected with monoclonal antibodies to ia alpha and beta chains. | studies with two monoclonal antibodies (da6.147 and da6.231) which react, respectively, with isolated human ia alpha and beta chains are reported. both antibodies detect epitopes expressed on all dr-heterozygous and dr-homozygous cell lines tested (n = 17) and bind to the epstein-barr virus-negative cell line ramos. ia subunit specificity of the antibodies was determined by an adaptation of an electroblot technique which transfers separated ia chains from polyacrylamide gels to nitrocellulose pa ... | 1982 | 6185348 |
| influence of ataxia telangiectasia gene dosage on bleomycin-induced chromosome breakage and inhibition of replication in human lymphoblastoid cell lines. | 1982 | 6173245 | |
| heterogeneity of allogeneic restriction of human cytotoxic t cell clones specific for epstein barr virus. | clones of human cytotoxic t cells (tc) specific for epstein barr virus (ebv) were isolated from peripheral blood lymphocyte (pbl) cultures stimulated repeatedly with autologous ebv-transformed lymphoblastoid cell line (lcl) cells in vitro. the method employed to clone ebv-specific tc was a limiting dilution technique utilizing t cell growth factor (tcgf). the ebv specificity of tc clones was determined by showing that they were significantly cytotoxic for autologous lcl cells but not for either ... | 1982 | 6173428 |
| production and characterization of marmoset lymphoblastoid interferon. | we describe the production of marmoset lymphoblastoid cell interferon (ifn). optimal yields of ifn were obtained when ebv-transformed lymphoblastoid cell lines (lcl) at a cell density of 1 x 10(6)/ml were incubated with 100 hau sendai virus/ml for 24 h. sendai-virus-induced marmoset lymphoblastoid cell ifn was acid-stable and exerted antiviral activity on both homologous and heterologous human cells, thus allowing its classification as ifn alpha. marmoset lymphoblastoid cell ifn did not inhibit ... | 1982 | 6174462 |
| nasopharyngeal carcinoma in american children: epstein-barr virus-specific antibody titers and prognosis. | seven american juvenile patients with undifferentiated or nonkeratinizing nasopharyngeal carcinoma (npc) were examined serially for epstein-barr virus (ebv)-specific antibody spectra and titers in sera. at diagnosis, all showed antibody patterns characteristic of npc: i.e., high titers of igg antibodies to viral capsid antigen (vca) and to the diffuse (d) component of the early antigen complex. six patients had iga antibodies to vca, and four to the d component. in the patients who responded to ... | 1982 | 6174465 |
| interferon production potentials of various human lymphoblastoid cell lines. | a number of human lymphoblastoid cells were examined concerning their ability to produce spontaneously liberated and virus-induced interferon (ifn). it was found that, in addition to b cells, various t and nont-nonb lymphoblastoid cells responded well to sendai virus infection to form ifn, the characterization of which has been recently reported (20). one b lymphoblastoid cell line from an infectious mononucleosis (im) patient produced a large amount of ifn-alpha and might become an alternative ... | 1982 | 6188944 |
| diagnosis of cutaneous t cell lymphoma by use of monoclonal antibodies reactive with tumor-associated antigens. | two murine monoclonal antibodies (be1 and be2), produced by using leukemic helper t cells from a patient with cutaneous t-cell lymphoma (ctcl) as immunogens, reacted selectively with ctcl lymphocytes and some transformed cultured lymphocytes, as determined by radioimmunoassay (ria) and indirect immunofluorescence (iif). be1 reacted significantly (p less than or equal to 0.001) with leukemic ctcl lymphocytes and with ctcl cells from infiltrated lymph nodes (ria, mean +/- sd = 776 +/- 275 cpm), as ... | 1982 | 6217214 |
| helper cell-independent cytotoxic clones in man. | we report here a class of helper cell-independent cytotoxic t cell (hitc) clones in man that can proliferate in response to antigenic stimulation as well as mediate cytotoxicity. hitc appear to be rare among clones derived from primary in vitro allosensitized culture, but constitute the majority of clones derived from cells sensitized to autologous epstein-barr virus-transformed lymphoblastoid cell lines. the implications of the derivation and function of hitc clones are discussed. | 1982 | 6217275 |
| transformation-associated tumor antigens. | 1982 | 6190362 | |
| reactivation of epstein-barr virus in pregnant women: social factors, and immune competence as determinants of lymphoproliferative diseases-a hypothesis. | in normal pregnant women, reactivation of epstein-barr virus (ebv) frequently occurs. cellular immune responses are apparently suppressed, but high titer ebv-specific antibodies of igg class may compensate. the antibodies cross the placenta and protect the infant against primary infection for many months. reactivation of ebv in pregnancy and oral excretion of virus by normal pregnant women could be one explanation why young children in large sibships (among families of low social classes) become ... | 1982 | 6285155 |
| epstein-barr virus vca igm and ebna igg antibodies titered by immunofluorescence in microplates. a semi-automated method based on microtiter system. | we describe methods of immunofluorescence in microplates for titration of ebv vca-igm and ebna-igg antibodies and compare the sensitivity of the methods with glass slides and with microplates, showing the reproducibility of the methods. results of vca-igm and -igg, ea and ebna titers obtained in four groups of patients are given, comprising cases of infectious mononucleosis, renal graft recipients, lymphoproliferative diseases and controls. | 1982 | 6285158 |
| in vitro transcription of two epstein-barr virus specified small rna molecules. | cloned dna from the ecori j fragment of ebv has been used as template for in vitro transcription experiments using cell-free extracts prepared from hela or kb cells. two ebv specific rnas each about 175 bases in length were synthesised and nuclease 51 mapping experiments determined that these in vitro products corresponded precisely to the in vivo species obtained from raji cells. these two rna molecules are transcribed by rna polymerase iii and in common with other pol iii-synthesised rnas the ... | 1982 | 6285300 |
| a prospective study of oropharyngeal excretion of epstein-barr virus in renal homograft recipients. | this prospective study was undertaken to examine the incidence of oropharyngeal excretions of epstein-barr virus (ebv) in the period after transplantation in human renal allograft recipients. throat gargles from 23 recipients were tested for ebv. five patients were already ebv excretors when the first specimens were tested. of the remaining 18 patients 15 converted to positive within the first 7 months following transplantation. three patients never excreted ebv in their throat gargles. this pro ... | 1982 | 6285453 |
| metronidazole in the treatment of anginose infectious mononucleosis. | 1982 | 6285454 | |
| infection of the human t-cell-derived leukemia line molt-4 by epstein-barr virus (ebv): induction of ebv-determined antigens and virus reproduction. | 1982 | 6285596 | |
| changes in the expression of fc receptor produced by induction of epstein-barr virus in lymphoma cell lines. | 1982 | 6285610 | |
| spurious rapid infections mononucleosis test results in non-infectious mononucleosis sera. the role of high-titer horse agglutinins. | the present report-describes serologic data from five individuals without infectious mononucleosis (im) who had very high levels of horse red blood cell agglutinins (1:896-1:7168) that were unusual in that they were minimally absorbed by either guinea pig kidney or beef red blood cell suspensions. agglutination of sheep red blood cells was negative, and epstein-barr virus (ebv)-specific serodiagnostic tests revealed evidence for long past primary infections. in one of the five cases, serologic f ... | 1982 | 6285691 |
| epstein-barr virus antibody in childhood hodgkin's disease. | fifteen patients with childhood onset of hodgkin's disease were studied for prevalence and quantity of epstein-barr virus (ebv) antibody to learn about the relationship between infection with ebv and hodgkin's disease. findings indicated that, compared with normal child control subjects, prevalence of ebv antibody is not increased in hodgkin's disease, but the quantity of antibody increases as the duration of hodgkin's disease increases. it seems that ebv plays no role in the cause of hodgkin's ... | 1982 | 6285693 |
| acyclovir in mouse cytomegalovirus infections. | a virus-specified thymidine kinase appears to be a general requirement for herpes virus susceptibility to the antiviral effect of acyclovir. surprisingly, mouse cytomegalovirus (mcmv), which does not encode for a thymidine kinase, is exquisitely sensitive to the drug both in vitro and in vivo. the drug is active against the virus in the absence of a cellular thymidine kinase and the antiviral activity is not diminished in the presence of excess thymidine or a variety of nucleosides and deoxynucl ... | 1982 | 6285701 |
| perspectives on interactions of acyclovir with epstein-barr and other herpes viruses. | acyclovir [9-(2-hydroxyethoxymethyl)guanine] inhibits epstein-barr virus (ebv) replication in lymphoblastoid cells at concentrations nontoxic to cellular growth. the mode of action of the drug against ebv differs from the mechanism described in herpes simplex virus systems. due to the absence of virus-specified thymidine kinase, the drug is poorly phosphorylated in ebv-infected cells. the extent of monophosphorylation is similar both in mock-infected and ebv-infected cells. despite weak phosphor ... | 1982 | 6285710 |
| treatment of life-threatening epstein-barr virus infection with acyclovir. | two pediatric patients with life-threatening epstein-barr virus infections were studied immunologically and treated with acyclovir [9-(2-hydroxyethoxymethyl) guanine]. the patient with chronic active epstein-barr virus infection who experienced massive hepatosplenomegaly, pancytopenia, and failure to thrive demonstrated abnormalities of t and b lymphocytes. a second patient, with the x-linked lymphoproliferative syndrome, experienced a rapidly fatal course of acute epstein-barr virus infection w ... | 1982 | 6285718 |
| effect of acyclovir on herpes simplex virus replication in a persistently infected human lymphoblastoid cell line (p3hr-1). | 1982 | 6285737 | |
| replication of epstein-barr virus dna in lymphoblastoid cells treated for extended periods with acyclovir. | 1982 | 6285738 | |
| expression of epstein-barr virus genome in nude-mouse-growth nasopharyngeal carcinoma cells. | 1982 | 6292325 | |
| effects of n-butyrate on epstein-barr virus-carrying lymphoma lines. | n-butyrate has been shown to induce epstein-barr virus (ebv) antigen synthesis in certain ebv-carrying lymphoma lines (luka et al., 1979). we have studied the effect of n-butyrate on two ebv-positive burkitt lymphoma lines by immuno-fluorescence and electron microscopy. in the producer line p3hr-1, the drug induced not only early antigen (ea) and virus capsid antigen (vca) synthesis, as shown before, but also increased the number of cells containing virus particles. the transition from vca expre ... | 1982 | 6292341 |
| hypomethylation of epstein-barr virus dna in the nonproducer b-cell line ebr. | the conversion of the epstein-barr virus-negative ramos cell line has previously been shown to result in an epstein-barr virus-positive non-virus-producer cell line, ebr. we report here that epstein-barr virus dna from ebr alone among several cell lines examined was totally unmethylated at three of four sites containing guanine plus cytosine which were tested. this is in direct contrast to reports of high degrees of methylation in the dnas of other animal viruses, including herpesviruses, isolat ... | 1982 | 6292457 |
| deletion of the nontransforming epstein-barr virus strain p3hr-1 causes fusion of the large internal repeat to the dsl region. | the nontransforming epstein-barr virus (ebv) strain p3hr-1 is known to have a deletion of sequences of the long unique region adjacent to the large internal repeats. the deleted region is believed to be required for initiation of transformation. to establish a more detailed map of the deletion in p3hr-1 virus, sali-a of the transforming strain m-aba and of p3hr-1 virus was cloned into the cosmid vector phc79 and multiplied in escherichia coli. the cleavage sites for bamhi, bglii, ecori, psti, sa ... | 1982 | 6292473 |
| epstein-barr virus dna xii. a variable region of the epstein-barr virus genome is included in the p3hr-1 deletion. | the p3hr-1 subclone of jijoye differs from jijoye and from other epstein-barr virus (ebv)-infected cell lines in that the virus produced by p3hr-1 cultures lacks the ability to growth-transform normal b lymphocytes (heston et al., nature (london) 295:160-163, 1982; miller et al., j. virol. 18:1071-1080, 1976; miller et al., proc. natl. acad. sci. u.s.a. 71:4006-4010, 1974; ragona et al., virology 101:553-557, 1980). the p3hr-1 virus was known to be deleted for a region which encodes rna in laten ... | 1982 | 6292475 |
| long internal direct repeat in epstein-barr virus dna. | the nucleotide sequence of the long internal reiteration. ir1, of epstein-barr virus dna has been determined. the repeat unit is 3,071 base pairs which are 66.8% guanine plus cytosine. there is a ccaat sequence 39 nucleotides 5' to a tataa, which could indicate a promotor for transcription. the longest open reading frame is 1,124 base pairs. also within ir1 is a sequence homologous to the papovavirus origin of dna replication. the ori-like sequence is within a long palindromic region which is 50 ... | 1982 | 6292491 |
| simple repeat sequence in epstein-barr virus dna is transcribed in latent and productive infections. | the bamhi k region of epstein-barr virus dna is transcribed in latently infected cells from burkitt tumors and in growth-transformed b-lymphocytes latently infected with epstein-barr virus. we determined the nucleotide sequence of a 1,153-base pair hinfi fragment in bamhi fragment k from the b95-8 epstein-barr virus isolate. the fragment contains a remarkable 708-base pair simple sequence repeat array, designated ir3, which is composed of only three nucleotide triplet elements: ggg, gca, and gga ... | 1982 | 6292493 |
| acute polyarthritis associated with active epstein-barr virus infection. | nine patients with an initial onset of symptoms of acute arthritis within the preceding four weeks were enrolled in a prospective serological study with clinical follow-up for six months to two years. four adults with chronic rheumatoid arthritis and ten healthy adults were similarly studied. serial titers measured included antibodies to epstein-barr virus (ebv) antigens, group b coxsackieviruses, rubella virus, cytomegalovirus, and herpes simplex virus. serological evidence of active ebv infect ... | 1982 | 6292536 |
| human hybridomas constructed with antigen-specific epstein-barr virus-transformed cell lines. | a 6-thioguanine-resistant, human lymphoblastoid b-cell line (gm1500 6tg a-11; igg secreting) was mutagen-treated with low-level gamma-irradiation and selected for ouabain resistance. one line showing 10,000-fold higher drug resistance, designated kr-4, was fused with an epstein-barr virus-transformed, cloned, b-lymphocyte cell line (b6) producing antitetanus toxoid (tt) antibody (igm), and the hybrids were selected in hypoxanthine/aminopterin/thymidine medium containing 10 microm ouabain. surviv ... | 1982 | 6292911 |
| epstein-barr virus: a human pathogen inducing lymphoproliferation in vivo and in vitro. | epstein-barr virus (ebv) is a pathogen that is associated with several diseases in humans. it causes most heterophile-positive cases of infectious mononucleosis. the virus is associated with another lymphoproliferative disease, african burkitt's lymphoma, and with a malignancy of the nasopharynx, nasopharyngeal carcinoma. ebv appears to be a causative agent for some fatal lymphoproliferations in congenitally immunodeficient people. one proliferating cell type in infectious mononucleosis is an eb ... | 1982 | 6293034 |
| the paul-bunnel test revisited. | 1982 | 6293035 | |
| non-a, non-b hepatitis after open-heart surgery in sweden. | in recent years evidence has emerged that most post-transfusion hepatitis is caused by one or more previously unknown agents named non-a, non-b. a prospective investigation was made of 74 patients who underwent open-heart surgery. only volunteer blood was used for transfusions. transfusion-associated hepatitis appeared in 15 (20%) of the patients 4-12 weeks after the operation. in no case was the hepatitis found to be caused by hepatitis b, a or epstein-barr virus. one patient had a cytomegalovi ... | 1982 | 6293045 |
| etiology of childhood infectious mononucleosis syndrome in singapore. | 1982 | 6293096 | |
| transformation of lymphocytes by epstein-barr virus requires only one-fourth of the viral genome. | 1982 | 6293180 | |
| a fraction of epstein--barr virus virion dna is methylated in and around the ecori-j fragment. | 1982 | 6293192 | |
| human sessile b lymphocyte marker defined by a monoclonal antibody. | 1982 | 6293283 | |
| mechanisms of viral tumorigenesis. | 1982 | 6293289 | |
| some factors of immunopathogenesis of infectious mononucleosis in children. | sixty-eight children with the symptoms of infectious mononucleosis were examined. in 25 of them anti-ebv antibodies were found and in 10 heterophilic antibodies. in serum of 16 children sero-positive to ebv a, m, g, d immunoglobulins were determined as well as t and b lymphocytes. a statistically significant increase in the number of b-iga, b-igg and b-igm lymphocytes, augmentation of t lymphocyte number and polyclonal hypergammaglobulinemia were detected. | 1982 | 6293402 |
| epidemiologic approaches to cancer etiology. | 1982 | 6293517 | |
| epstein-barr virus in nasopharyngeal and salivary gland carcinomas of greenland eskimoes. | biopsy specimens from nasopharyngeal carcinomas (npc) or salivary-gland carcinomas (sgc) in greenland eskimoes were examined for the presence of epstein-barr virus (ebv) dna and sera from the patients were tested for ebv-specific antibody titres. six out of 7 npcs and one from an undifferentiated sgg were positive for ebv dna. the ebv-specific antibody spectra and titres of the patients with npc or undifferentiated sgg conformed to the results of earlier studies in other high-incidence areas. | 1982 | 6293523 |
| antibodies from eb-virus-transformed lymphocytes of lymph nodes adjoining lung cancer. | 1982 | 6293524 | |
| a kinetics model of abrin binding in a virus transformed lymphocyte cell culture. | 1982 | 6293634 | |
| [antibodies to the epstein-barr virus in pregnant women with suspected teratogenic virus infection]. | 1982 | 6293726 | |
| lymphocyte subsets in hypogammaglobulinaemia. | 1982 | 6293743 | |
| clonal analysis of a human lymphoblastoid cell line (b17) secreting antibody to n-acetyl-d-glucosamine. | in this paper we analyse the clonal composition of a human lymphoblastoid b-cell line secreting igm/k antibody to n-acetyl-d-glucosamine, the immunodominant sugar of group-a-streptococcal carbohydrate. besides non-antibody secreting cells, the line consists of two clonotypes of antibody-secreting cells: b17 cells producing over 90% and f6 cells producing less than 10% of the antibody in the supernatant. the proportions of b17 and f6 cells in the cell line seem to be similar to the proportion of ... | 1982 | 6293967 |
| in vitro induction of anti-intermediate filament antibody in lymphocyte cultures by epstein-barr virus. | serum antibodies reactive with intermediate filaments of the cytoskeleton (anti-if antibodies) are often present in infectious mononucleosis, some other viral diseases, and rheumatoid arthritis. the mechanism of their production is not known, but it is possible that the formation of this and other autoantibodies result from polyclonal activation of b-cells. peripheral blood mononuclear cells from subjects with or without serum anti-if antibody were therefore cultured in the presence or absence o ... | 1982 | 6293970 |
| [a rapid method for the determination of virus-specific igm antibodies]. | a newly developed ion exchange chromatography is reported; it is a simple and quick method to separate a highly purified igm fraction from blood serum. the immunofluorescence test was used to demonstrate that these fractions contain only virus-specific igm antibodies, but no demonstrable igg antibodies, which can often block the specific igm reactivity by competitive inhibition. the specificity of the reaction was tested on sera from 145 persons: the igm test was always positive in clinically pr ... | 1982 | 6293973 |
| epidemiological aspects of acute viral hepatitis in drug abusers. | the epidemiological features of acute symptomatic viral hepatitis were examined in 151 consecutive, hospitalized drug abusers. hepatitis b was diagnosed in 101 patients (66.8%), hepatitis a in 13 (8.6%) and non-a, non-b hepatitis in 35 (23.1%). non-a, non-b hepatitis was significantly more prevalent among drug abusers than in an age-matched control population of non-drug abusers. moreover, the mean duration of parenteral drug abuse was significantly lower among non-a, non-b cases than in patient ... | 1982 | 6293974 |
| a comparative study of complex mitochondrial dna in human lymphocytes transformed by epstein-barr virus and pha. | mitochondrial dna (mt dna) molecules, isolated from normal human lymphocytes, lymphocytes stimulated with phytohemagglutinin (72 h) and a cultured burkitt's lymphoma cell line, were examined by electron microscopy. only monomeric forms of mt dna were found in normal human lymphocytes, whereas in the other cell types, catenated forms were also observed. unicircular complex forms were apparently absent in all the cell types studied, suggesting that these forms are not a common malignant transforma ... | 1982 | 6289593 |
| epstein-barr virus antigens-a challenge to modern biochemistry. | 1982 | 6289637 | |
| new oncologic associations for the epstein-barr virus. | 1982 | 6289684 | |
| a new nucleoside analog, 9-[[2-hydroxy-1-(hydroxymethyl)ethoxyl]methyl]guanine, highly active in vitro against herpes simplex virus types 1 and 2. | a novel nucleoside analog, 9-[[2-hydroxy-1-(hydroxymethyl)ethoxy]methyl]-guanine (biolf-62), was found to have potent antiviral activity against herpes simplex virus types 1 and 2 at concentrations well below cytotoxic levels. for example, the patton strain of herpes simplex virus type 1 was susceptible at concentrations 140- to 2,900-fold below that which inhibited cell division by 50%, depending upon the cell line used for assay. different herpesvirus strains varied considerably in their susce ... | 1982 | 6289741 |
| [rheumatoid synovitis (problems of immunomorphology and immunopathogenesis)]. | the immunopathogenesis of rheumatoid synovitis (rs) is assumed to be as follows: antigen x interacts with the organism of subjects genetically predisposed to rheumatoid arthritis (ra) as a result of which the immune response regulation is disturbed ("immunological discomfort") leading to changes in proliferation and differentiation of immunocompetent cells immunologically manifested by disturbed ratios of lymphocyte subpopulations and morphologically by immune inflammation of synovial membranes, ... | 1982 | 6289782 |
| activation of epstein-barr virus expression in human lymphoblastoid p3hr-1 and raji cells with propionic acid and with culture fluids of propionic acid-producing anaerobes. | epstein-barr virus (ebv)-associated early (ea) and virus capsid antigens (vca) were efficiently induced in the viral genome-carrying human lymphoblastoid cells, p3hr-1 and raji, by the culture fluids of propionibacterium acnes, p. avidum, p. lymphophilum and arachnia propionica, the anaerobes which are commonly seen among the normal flora of man. the active principle for ebv-induction in the 2 cell lines was the propionic acid produced by the microbes and such activity was shown to correlate wit ... | 1982 | 6290028 |
| [immunoenzymatic assay of igg and igm to ebv and cmv in sera of patients with virus hepatitis and normal control]. | 1982 | 6290092 | |
| humoral immune response in epstein-barr virus infections. i. elevated serum concentration of the igg1 subclass in infectious mononucleosis and nasopharyngeal carcinoma. | using radial immunodiffusion we measured igg subclass concentrations and studied their distribution in serum samples from patients with infectious mononucleosis (im) and nasopharyngeal carcinoma (npc), two epstein-barr virus (ebv)-associated diseases, in comparison with two control groups [completely anti-ebv negative persons and subjects carrying antibodies to the viral capsid antigen (vca)]. antibody titres to vca and to the early antigen (ea) were determined by indirect immunofluorescence and ... | 1982 | 6290114 |
| quantitative comparison of epstein-barr virus receptor expression on sigm and sigg cell lines and b-cell lymphoma biopsies. | over 50 b-cell derived lines and b-cell lymphoma and leukemia biopsies were screened for expression of the epstein-barr virus (ebv) receptor. the 13 sigm-positive lines bound more than five times as much virus as the six igg lines. among the biopsies, the 17 sigm, 11 sigm and sigd, and seven sigg expressing biopsies were further divided according to expression of the c3 receptor. c3 receptor-positive biopsies, which expressed sigm alone or along with sigd, had the largest subpopulation of cells ... | 1982 | 6290303 |
| neutralization of epstein-barr virus by nonimmune human serum. role of cross-reacting antibody to herpes simplex virus and complement. | these studies were carried out to investigate the mechanism of neutralization of purified epstein-barr virus (ebv) by fresh human serum from normal individuals lacking antibody to the ebv viral capsid (vca) and nuclear antigens (ebna). such individuals thus lack serological evidence of immunity to ebv. although an enzyme-linked immunosorbent assay (elisa) with highly purified immobilized ebv detected low levels of igg antibody reactive with ebv in these normal nonimmune sera, this antibody faile ... | 1982 | 6290536 |
| immune response to alien histocompatibility antigens on epstein-barr virus transformed cells. | expression of alien histocompatibility antigens on epstein-barr virus transformed, cultured lymphoblastoid cell lines (lcl), which were established from normal peripheral blood lymphocytes (pbl), was studied by means of mixed lymphocyte reaction (mlr), cell mediated lysis (cml) and primed lymphocyte typing (plt). stimulation of pbl by autologous lcl resulted in some mlr responses and generation of cytotoxic effector cells against autologous lcl. restimulation of pbl by 16 individual allogeneic p ... | 1982 | 6290715 |
| expression of target antigen for epstein-barr virus-specific cytotoxic t cells on bjab cells freshly infected with ebv. | the target antigen for epstein-barr virus (ebv)-specific cytotoxic t cells (tc) was expressed on bjab cells exposed to the b95-8 strain of ebv for at least one hour. ultraviolet-light (uv)-irradiated noninfectious b95-8 virus also induced the target antigen on bjab cells. cold target competition tests suggested that the target antigen expressed on ebv-infected bjab cells was distinct from the lymphocyte-detected membrane antigen (lydma) which was also recognized by the ebv-specific tc and expres ... | 1982 | 6290853 |
| epstein-barr virus is not associated with head and neck neoplasms other than nasopharyngeal carcinoma. | 1982 | 6290911 | |
| epstein-barr virus (ebv)-antibody profile in an infant congenitally infected by the ebv. | 1982 | 6290976 | |
| human antibody to ofa-i, a tumor antigen, produced in vitro by epstein-barr virus-transformed human b-lymphoid cell lines. | we established two long-term human b-lymphoblastoid cell lines (l55 and l72) transformed by epstein-barr virus that produced igm kappa antibodies to the human tumor antigen, ofa-i. periphral blood lymphocytes obtained from melanoma patients were used as the source of the b lymphocytes. antibody specificity was determined by the immune adherence assay using various human cancer and noncancer tissues as targets. l55 antibody (designated anti-ofa-i-1) reacted with a variety of human tumor types whe ... | 1982 | 6291057 |
| stable expression in mouse cells of nuclear neoantigen after transfer of a 3.4-megadalton cloned fragment of epstein-barr virus dna. | all cells that harbor the epstein-barr virus (ebv) genome contain a neoantigen in the nucleus (ebna). by transfection we located a segment of the genome that encodes or induces an antigen serologically related to ebna. the responsible genes are found in the 3.4-megaldalton bamhi fragment k of ebv dna, specifically in the left 1.9 megadaltons represented by hindiii fragment i1. mouse ltk- cells were cotransformed with recombinant plasmids, containing the herpes simplex virus thymidine kinase gene ... | 1982 | 6291059 |
| mapping of polypeptides encoded by the epstein-barr virus genome in productive infection. | over 30 viral-specified polypeptides are translated in vitro from rna of cells productively infected with epstein-barr virus (ebv). the polypeptides map to sites in ebv dna by hybrid selection. almost all of the polypeptides are reactive with ebv immune human serum. several of the polypeptides are part of the early antigen complex. two others are likely to be major structural components of the virus. genes encoding persistent early and late polypeptides are intermixed through most of the ebv gen ... | 1982 | 6291060 |
| electron microscopic detection of periodate reactive complex carbohydrates in human t and b lymphocytes. | the periodic acid-thiocarbohydrazide-silver proteinate (pa-tch-sp) technique, which has the same cytochemical significance as pas staining of light microscopy, was undertaken to reveal the distribution of complex carbohydrates with vicinal glycols in human lymphocytes in several conditions at the ultra-structural level. the pa-tch-sp method stained the clustered and the scattered cytoplasmic granules, golgi apparatus and glycogen particles of lymphocytes. in the buffy coat, the lymphocytes with ... | 1982 | 6291141 |
| inhibition of epstein-barr virus-associated nuclear antigen (ebna) induction by (2',5')oligoadenylate and the cordycepin analog: mechanism of action for inhibition of ebv-induced transformation. | 1982 | 6291233 | |
| epstein-barr virus in normal pregnant women. | acquired immune suppression accompanying normal pregnancy may be associated with reactivation of epstein-barr virus (ebv). pregnant women with reactivated ebv having anti-ea antibodies show high titers of antiviral capsid antigen (vca) geometric mean titers (gmt) of 522 versus 170 in those lacking anti-early antigen (ea). among twenty-seven seropositive women at parturition, 17 (63%) had generated antibody to ea, and all 27 (100%) demonstrated significant increases in antibody to vca (p less tha ... | 1982 | 6291416 |
| infectious mononucleosis in singapore. | 1982 | 6291444 | |
| mode of action of acyclovir triphosphate on herpesviral and cellular dna polymerases. | the effect of 5'-triphosphate of acyclovir (acv) on dna polymerases of two human herpes-viruses, herpes simplex virus type-1 (hsv-1) and epstein-barr virus (ebv) as well as human cellular dna polymerases alpha and beta has been examined. of the enzymes tested, hsv-1 dna polymerase was the most sensitive to inhibition by acyclovir triphosphate (acvtp). the ebv dna polymerase and dna polymerase beta were less sensitive. acvtp inhibition was competitive with dgtp with ki values of 0.03, 0.15, 9.8 a ... | 1982 | 6291456 |
| epstein-barr virus superinduces a new human b cell differentiation antigen (b-last 1) expressed on transformed lymphoblasts. | we have developed a monoclonal antibody that detects the first human b-cell-specific differentiation antigen expressed on transformed b lymphoblasts. the antigen is termed b-last 1 and is found on b cells transformed in vitro with epstein-barr virus (ebv) and pokeweed mitogen; in vivo with ebv and antigen; and on neoplastic b cells from chronic lymphocytic leukemia and poorly differentiated lymphoma. the antigen has a molecular weight of 45,000 and was not found on cells of t, null or myeloid li ... | 1982 | 6291768 |
| human ia-like-antigen-expressed cutaneous t cell lymphoma associated with epstein-barr virus infection. | 2 cases of cutaneous t cell lymphoma are reported. in both cases immunological studies revealed that the tumor cells from the cutaneous lesions had human ia-like antigen on the cell surface, which was considered to be alloantigen primarily present on b lymphocytes. serum examination showed an extremely high titer of two types of antibody against epstein-barr (eb) virus in the 1st case and a relatively high titer of one type of antibody in the 2nd case, suggesting that the patients had eb virus i ... | 1982 | 6292010 |
| studies on the toxicity and binding kinetics of abrin in normal and epstein-barr virus-transformed lymphocyte culture-i. experimental results - 4. | the effects of treatment with varying doses of abrin, a d-galactose binding lectin, on dna and protein synthesis of normal and epstein-barr virus (ebv)-transformed lymphocytes have been previously investigated. using data on ebv-transformed lymphocyte cell density as a function of both time and dose of abrin, the authors introduced the concept of self- and cross-coupling metabolic variables as a means of understanding how abrin affected dna and protein uptake. in this paper, the self-coupling co ... | 1982 | 6292014 |
| cytotoxic effector cells from infectious mononucleosis patients in the acute phase do not specifically kill epstein-barr virus genome-carrying lymphoid cell lines. | we describe a study in which we investigated the cytotoxic activities of thymusderived (t) lymphocytes and natural killer cells against epstein-barr virus (ebv) genome-carrying lymphoid cell lines. purified subpopulations of lymphocytes from eight patients with infectious mononucleosis and six healthy normal ebv-seropositive donors were tested. enriched t-cells were obtained by passing purified whole blood lymphocyte preparations through human immunoglobulin-anti-immunoglobulin-coated glass bead ... | 1982 | 6292093 |
| the interaction of non-transforming epstein-barr virus (ebv) with cell-associated ebv dna in superinfected lymphoblastoid cell lines. | two human lymphoblastoid cell lines were established by the transformation of human cord-blood lymphocytes with transforming epstein-barr virus (ebv). one cell line (hlb-r1) was established with ebv obtained after the superinfection of raji cells with hr-1 ebv and the other (hlb-bl) was established from b95-8 ebv-infected human cord-blood lymphocytes. both the hlb-r1 and hlb-b1 lines were susceptible to superinfection with hr-1 ebv. we found that ebv dna was replicated in the superinfected cell ... | 1982 | 6292115 |
| epstein-barr virus strain-specific differences in transformed cell lines demonstrated in growth characteristics, induction of viral antigens and adcc susceptibility. | 1982 | 6292116 | |
| epstein-barr virus induction by a serum factor: iv. ubiquitous occurrence of the factor within vertebrates and its interaction with defined lymphoid cell lines. | we have recently reported the existence of a serum factor that induces epstein-barr virus antigens in raji cells (bauer et al., 1982b, c). here we demonstrate the ubiquitous presence of this factor in sera of vertebrates of all five classes. furthermore, we tested 48 ebv-genome-positive lymphoid cell lines of different origins for responsiveness to the serum factor. only cells from lines of two groups, i.e. burkitt's lymphoma and ebv-transformed marmoset lines, responded to the factor, whereas h ... | 1982 | 6292119 |
| atp-dependent lysosomal cystine efflux is defective in cystinosis. | lysosomes containing large amounts of the amino acid, cystine, were obtained from transformed, cultured, human lymphoblasts which had been exposed to cystine dimethyl ester. lysosomal cystine efflux was greatly enhanced by exogenous atp in cell lines from normal individuals. cystine efflux was unresponsive to atp in lysosomes from individuals with the disorder, cystinosis. efflux of cystine from normal cell lysosomes was inhibited by both the atp analog, 5-adenylylimidodiphosphate, and the proto ... | 1982 | 6292178 |
| quantification of an epstein-barr virus-associated membrane antigen component. | a method is described for the preparation of a 125i-labelled membrane antigen (ma) component (gp340) from b95-8 cell membranes using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page). good yields of antigenic material were obtained when renaturation of the [125i]gp340 was carried out by removal of sds in the presence of urea and subsequent removal of the urea. the availability of purified, radiolabelled gp340 has provided the essential basis for the development of a radioimmu ... | 1982 | 6292251 |
| immune deficiency in the x-linked lymphoproliferative syndrome. i. epstein-barr virus-specific defects. | eleven males with xlp were evaluated for ebv-specific antibodies during periods of 2 to 7 yr. variable responses to ebv-specific antigens were found. all 11 patients had subnormal anti-ebna titers, which probably reflected a t cell deficiency. the patients showed four different patterns in their anti-vca response: 1) two boys who had experienced malignant lymphoma mounted no antibodies at all; 2) two patients showed intermittent anti-vca titers; 3) four males had persistently elevated anti-vca t ... | 1982 | 6292290 |
| immune deficiency in the x-linked lymphoproliferative syndrome. ii. immunoregulatory t cell defects. | surface phenotypic markers and the function of lymphocytes in patients affected with the x-linked lymphoproliferative syndrome (xlp) were studied. this syndrome is characterized by a defective response to infection with epstein barr virus (ebv). normal numbers of b and t cells were detected with anti-ig and monoclonal okt3 antisera, respectively. t cell subset values, however, were persistently altered: cells reacting with okt8 were significantly elevated in five of nine patients, accompanied by ... | 1982 | 6292291 |
| [detection of iga serum antibodies to eb virus vca in patients having survived nasopharyngeal cancer for over 15 years (author's transl)]. | 1982 | 6286059 | |
| burkitt's lymphoma with 2/8 translocation: a case report with special reference to the clinical features. | a case of japanese burkitt's lymphoma (bl) with a t(2; 8) (p11; q24), a variant translocation in bl, is reported. the patient was a 45-year-old woman in whom a subcutaneous right axillary tumor first occurred. remission was not achieved despite extensive chemotherapy. of the four nonendemic bl, two endemic bl, and one nonendemic burkitt-type acute lymphocytic leukemia (all-l3) cases with a t(2; 8) reported so far, including the present case, four (two nonendemic and two endemic) were positive fo ... | 1982 | 6286099 |
| molecular interactions in human t-cell-mediated cytotoxicity to epstein-barr virus. i. blocking of effector cell function by monoclonal antibody okt3. | 1982 | 6286147 | |
| suppressor t cells activated by autologous b-cell derived lines inhibit blastogenic responses by peripheral lymphocytes to mitogens or autologous lymphoblastoid cell lines. | 1982 | 6286150 | |
| suppressor t-cell function in man: suppression of immunoglobulin production by the direct action of immunoregulatory t cells on the b cell in four separate, distinct systems. | 1982 | 6286193 | |
| [virus etiology of hodgkin's disease]. | 1982 | 6286265 | |
| various phenotypes of diabetes mellitus at ultimate outcome of acutely developed diabetic state induced by viral infection. | for 4 to 8 years we followed up 3 diabetic patients in whom the onset of diabetes seemed to be closely related to the well-documented epstein-barr virus infection (case 1) or coxsackie b4 virus infection (case 2, 3). although all developed acute ketosis-prone diabetes in the convalescent stage of the viral infections, the subsequent clinical courses were quite different from each other. case 1 has remained consistently insulin-dependent and associated with positive islet cell antibody, gastric p ... | 1982 | 6286296 |
| immunoglobulin secretion by human b lymphocytes exposed to herpes simplex type i antigen. | herpes simplex type i (hsv i) antigen was studied for its capacity to induce immunoglobulin(ig)-secreting cells in human peripheral-blood lymphocytes. ig-secreting cells were detected using an indirect hemolytic plaque assay. these results were compared to those obtained by using pokeweed mitogen (t-dependent) and epstein-barr virus (t-independent) to induce ig-secreting cells. the hsv i induction of ig synthesis was t-dependent, similar to the pokeweed-mitogen system. peripheral-blood mononucle ... | 1982 | 6286468 |
| a prospective study on infectious mononucleosis in childhood--symptoms, serology, epstein-barr-virus specific leukocyte migration inhibition. | the clinical course, serological changes and the development of the specific cell-mediated immune response to epstein-barr-virus (ebv), measured in terms of leukocyte migration inhibition, were followed in 40 children suffering from an ebv infection. the patients were followed for between six and 24 months. although the majority of the children were under six years of age, they presented a typical clinical course; heterophil antibodies could only be demonstrated in 60% of the cases. anti-vac-igm ... | 1982 | 6286500 |
| a monoclonal antibody with anti-burkitt lymphoma specificity. i. analysis of human haematopoietic and lymphoid cell lines. | 38-13 is a hybridoma-produced monoclonal rat igm which appears to define a burkitt's lymphoma-associated antigen (bla). in this paper, we described the reactivity of 38-13 with a panel of human haematopoietic and lymphoid cell lines. in indirect immunofluorescence (if) assays, 15 of 26 burkitt's lymphoma (bl) lines studied were clearly stained with 38-13 (from 13 to 100% positive cells) by microscope, with varying numbers of heavily labelled cells. in these positive cell lines, fluorescence-acti ... | 1982 | 6286514 |
| parotitis and pancreatitis complicating infectious mononucleosis. | 1982 | 6286529 |