Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| cleavage specificity of a myofibril-bound serine proteinase from carp (cyprinus carpio) muscle. | previously, we reported the purification and characterization of a myofibril-bound serine proteinase (mbp) from carp muscle (osatomi k, sasai h, cao m-j, hara k, ishihara t. comp biochem physiol 1997;116b:159-66). in the present study, the n-terminal amino acid sequence of the enzyme was determined, which showed high identity with those of other trypsin-like serine proteases. the cleavage specificity of mbp for dibasic and monobasic residues was investigated using various fluorogenic substrates ... | 1999 | 10582314 |
| identification of differentially expressed genes in con a-activated carp (cyprinus carpio l.) leucocytes. | a cdna library was constructed from the message rna (mrna) obtained from con a-induced head kidney (hk) leucocytes of carp (cyprinus carpio l.). differential screening of the cdna was carried out by hybridization against the total cdna probes from normal, con a-uninduced hk leucocytes or con a-induced hk leucocytes of carp. the differential expression patterns of certain cdna clones were confirmed by southern-blot and northern-blot analysis. single-pass of the sequencing analysis and homology se ... | 1999 | 10582319 |
| studies on the effect of growth hormone in vivo and in vitro on lipogenic enzymes and transaminases in a teleost anabas testudineus (bloch). | the specific activities of three lipogenic enzymes, malic enzyme (me), glucose-6-phosphate dehydrogenase (g6pdh) and isocitrate dehydrogenase (icdh), in liver and heart and two transaminases (ast & alt) in liver and muscle, were studied in response to the in vivo and in vitro administration of growth hormone (gh) in a teleost anabas testudineus. ovine growth hormone (ogh) in vivo significantly reduced the activities of lipogenic enzymes, except for heart g6pdh, which showed an increase at the hi ... | 1999 | 10596727 |
| evaluation of eukaryotic promoters for the construction of dna vaccines for aquaculture. | we evaluated fish promoters as an alternative to viral promoters in the construction of dna vaccines for aquaculture. a carp beta-actin promoter drove expression of the luciferase gene in live fish tissue to levels comparable to the cmvtk promoter. | 1999 | 10596750 |
| genetic diversity in crucian carp (carassius auratus). | a survey of restriction fragment polymorphism in mitochondrial dna of three subspecies of carassius auratus throughout four provinces in china was undertaken using 17 restriction enzymes. two carp, cyprinus carpio rubbrofuscus and cyprinus carpio carpio, were included as the outgroup. a total of 16 haplotypes was observed: 5 in tetraploids of c. auratus auratus; 8 in hexaploids of c. auratus auratus; and 2 in c. auratus gibelio and c. auratus cuvieri, respectively. the tetraploids and hexaploids ... | 1999 | 10626035 |
| ahtn and hhcb show weak estrogenic--but no uterotrophic activity. | the ubiquitous presence of the polycyclic musks ahtn (6-acetyl-1,1,2,4,4,7-hexamethyltetraline) and hhcb (1,2,4,6,7,8-hexahydro-4,6,6,7,8-hexamethylcyclopenta-gamma-2-b enzopyreen) in surface waters and their identification in human milk fat together with their polycyclic nature, which makes them potential candidates for interference with estrogen receptors, prompted us to assess these compounds for their potential estrogenic effects. we therefore investigated the effects of ahtn and hhcb in era ... | 1999 | 10630711 |
| expression of salmon corticotropin-releasing hormone precursor gene in the preoptic nucleus in stressed rainbow trout. | the behavior of genes encoding the corticotropin-releasing hormone (crh) precursor in response to stress has not been extensively studied in teleosts. to clarify this problem, crh cdnas were isolated from a hypothalamic cdna library of sockeye salmon, oncorhynchus nerka, by screening with pcr products amplified from the hypothalamic mrna with primers deduced from the sequence of the sucker crh precursor. two types of pcr products with a high degree of sequence homology were identified (crh-i and ... | 1999 | 9882547 |
| tgf-beta3 exists in bony fish. | a partial nucleotide sequence of transforming growth factor-beta3 (tgf-beta3) has been isolated from the siberian sturgeon (acipenser baeri), rainbow trout (oncorhynchus mykiss) and european eel (anguilla anguilla), confirming a ubiquitous presence in the ray-finned (actinopterygian) bony fish. the bony fish tgf-beta3 is highly conserved, with some 83-84% nucleotide identity (coding region) and 90-95% predicted amino acid identity to known homeotherm tgf-beta3's. far lower homologies are apparen ... | 1999 | 10614492 |
| characterization of ampa receptors on isolated amacrine-like cells in carp retina. | in amacrine-like cells freshly dissociated from crucian carp (carassius auratus) retina, we recorded whole-cell responses to rapid application of glutamate and kainate. currents induced by glutamate, but not kainate, usually showed extremely rapid desensitization, and the mean time constant for the decay of the responses to 10 mm glutamate was 2.77 ms. n-methyl-d-aspartate (nmda) failed to induce any current even with coapplication of glycine and removal of extracellular mg2 +. 1-(4-aminophenyl) ... | 1999 | 10594649 |
| cytological and biochemical alterations in carassius auratus hepatocytes from exposure to sediment containing dioxins and related compounds. | cytological and biochemical alterations of crucial carp (carassius auratus) hepatocytes were characterized after exposure to sediments from a lake contaminated with dioxins and other industrial chemicals. carp were exposed in 20 l water containing 25, 50, or 100 g of contaminated sediment for 2 and 4 weeks. ultrastructural changes in the liver were characterized by severe enlargement of hepatocytes. alterations in the cell included formation of condensed and irregular cell nucleus, polynuclei, d ... | 1999 | 10473792 |
| 11-ketotestosterone induces male-type sexual behavior and gonadotropin secretion in gynogenetic crucian carp, carassius auratus langsdorfii. | to determine if a gynogenetic teleost might have a sexually bipotential brain, we tested whether implantation of 11-ketotestosterone (kt) induces male-type sexual behavior and gonadotropin (gth) secretion in adult gynogenetic crucian carp, "ginbuna," carassius auratus langsdorfii. kt-implanted female ginbuna were tested for male spawning behavior by pairing them with a stimulus female in which sexual receptivity and attractivity were induced by prostaglandin f(2alpha) (pg) injection. when kt-imp ... | 1999 | 10417231 |
| modulation of desensitization at glutamate receptors in isolated crucian carp horizontal cells by concanavalin a, cyclothiazide, aniracetam and pepa. | in horizontal cells freshly dissociated from crucian carp (carassius auratus) retina, we examined the effects of modulators of glutamate receptor desensitization, concanavalin a, cyclothiazide, aniracetam and 4-[2-(phenylsulfonylamino)ethylthio]-2,6-difluoro-phenoxyacetam ide (pepa), on responses to rapid application of glutamate and kainate, using whole-cell voltage-clamp techniques. incubation of concanavalin a suppressed the peak response but weakly potentiated the equilibrium response of hor ... | 1999 | 10199629 |
| enantiomeric composition of the polycyclic musks hhcb and ahtn in different aquatic species | synthetic polycyclic musk fragrances are mainly represented by the compounds hhcb (galaxolide(tm)) and ahtn (tonalide(tm)). because of their volume of use and their bioaccumulation potential, there is concern with respect to their environmental safety. hhcb and ahtn are chiral compounds, and gas chromatography using modified cyclodextrins as chiral stationary phases coupled to high-resolution mass spectrometry enabled enantioselective analysis even under unfavorable matrix conditions. the gas ch ... | 1999 | 10561711 |
| energetic consequences of an inducible morphological defence in crucian carp. | crucian carp (carassius carassius) increases in body depth in response to chemical cues from piscivores and the deeper body constitutes a morphological defence against gape-limited piscivores. in the field, deep-bodied individuals suffer a density-dependent cost when competing with shallow-bodied conspecifics. here, we use hydrodynamic theory and swimming respirometry to investigate the proposed mechanism underlying this effect, high drag caused by the deep-bodied morphology. our study confirms ... | 1999 | 28307880 |
| effects of acute anoxia on heart function in crucian carp: importance of cholinergic and purinergic control. | the objective of this study was to characterize the effects of acute anoxia on contractile and electrical activity in the heart of an anoxia-tolerant fish species, the crucian carp (carassius carassius l.). responses of atrial and ventricular tissue or isolated cells to nacn, adenosine, and carbachol were determined to examine the effects of anoxia on cardiac performance and to clarify the possible role of local purinergic modulation and parasympathetic nervous control in the function of the ano ... | 1999 | 10444553 |
| na+/ca2+ exchange current in ventricular myocytes of fish heart: contribution to sarcolemmal ca2+ influx | influx of extracellular ca2+ plays a major role in the activation of contraction in fish cardiac cells. the relative contributions of na+/ca2+ exchange and l-type ca2+ channels to ca2+ influx are, however, unknown. using a physiological action potential as the command pulse in voltage-clamped heart cells, we examined sarcolemmal ca2+ influx through na+/ca2+ exchange and l-type ca2+ channels in crucian carp (carassius carassius l.) ventricular myocytes. when other cation conductances were blocked ... | 1999 | 10359679 |
| suppression of sustained and transient on signals of amacrine cells by gaba is mediated by different receptor subtypes. | intracellular recordings were made from amacrine cells in the isolated, superfused carp retina, and the effects of gamma-aminobutyric acid (gaba) on sustained and transient on signals of these cells were studied. exogenous gaba application partially suppressed the sustained response of on amacrine cells, which could be completely reversed by picrotoxin (ptx), a chloride channel blocker, and by bicuculline (bcc), a specific gaba(a) receptor antagonist. on the other hand, suppression by gaba of th ... | 1999 | 18763130 |
| kinematics and muscle dynamics of c- and s-starts of carp (cyprinus carpio l.). | an analysis is presented of body curvature, acceleration and muscle strain during fast-starts in the common carp (cyprinus carpio l.). c- and s-starts were filmed at 200 frames s-1 at 23 degreesc. curvatures and accelerations of mid-body axes were calculated from digitised outlines. maximum accelerations at 0.3 fl (fork length) from the snout were 54 m s-2 for c-starts and 40 m s-2 for s-starts. the total turning angle was approximately 150 degrees in c-starts. this angle was 70 degrees during e ... | 1999 | 9914147 |
| freshwater fish trypanosomes: definition of two types, host control by antibodies and lack of antigenic variation. | haemoflagellates of the genus trypanosoma are prevalent in freshwater fishes and are transmitted by leeches as vectors. as demonstrated by sequence comparisons of nuclear small subunit rrna genes, trypanosomes isolated from several fish species at different localities can be divided into at least 2 closely related types, designated type a and type b. a clone derived from a type a isolate from carp (cyprinus carpio) was used to study the anti-parasite immune response in specified pathogen-free ou ... | 1999 | 10633921 |
| the anti-estrogenicity of ah receptor agonists in carp (cyprinus carpio) hepatocytes. | cultured hepatocytes of female carp (cyprinus carpio) were coexposed for 4 days to 200 nm 17beta-estradiol (e2), and concentration ranges of nine known ah receptor (ahr) agonists: 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd), 3,3'4,4'5-pentachlorobiphenyl (pcb 126), 2,3'4,4'5-pentachlorobiphenyl (pcb 118), beta-naphthoflavone (bnf), benzo(a)pyrene (bap), benzo(a)anthracene (baa), diindolylmethane (dim), 6-methyl-1,3,8-trichlorodibenzofuran (mcdf) and hexachlorobenzene (hcb). tcdd caused a greater ... | 1999 | 10630570 |
| prevention by carp extract of myelotoxicity and gastrointestinal toxicity induced by 5-fluorouracil without loss of antitumor activity in mice. | the combined effects of 5-fluorouracil (5-fu) and carp (cyprinus carpio l.) extract on antitumor activity, myelotoxicity and gastrointestinal toxicity were examined in sarcoma 180-bearing mice. carp extract was found to prevent the occurrence of myelotoxicity as determined by the reduction of leukocyte number, and of gastrointestinal toxicity, as determined by the reduction of small intestinal weight, induced by 5-fu without loss of the antitumor activity of 5-fu. | 1999 | 10624860 |
| effects of nitric oxide, light adaptation and apb on spectral characteristics of h1 horizontal cells in carp retina. | the spectral characteristics of cone-driven horizontal cells of h1 subtype (h1 hcs) receiving main synaptic input from red-sensitive cones were studied in light- and dark-adapted retinae of carp. the spectral sensitivity profile of h1 hcs in dark-adapted retinae was practically the same as the absorption spectrum of red-sensitive cones. light-adaptation decreased the sensitivity preferentially in the short-wavelength (blue/green) region, resulting in a relative enhancement of the 617 nm peak. ap ... | 1999 | 10617322 |
| purification, characterization, and molecular cloning of carp hyosophorin. | carp hyosophorin (hsp) is purified from oocytes. it is a highly glycosylated protein (10% protein and 90% carbohydrate) of high molecular weight (>100 kda) and is localized in the cortical granules of oocytes. during cortical reaction carp hsp is exocytosed into the perivitelline space and is rapidly cleaved to the low-molecular-weight forms of 20 to 30 kda. the major part of carp hsp cdna is composed of tandem repeats, the repetitive domain. a repeat is 36 base pairs (bp) in length, which encod ... | 1999 | 10206660 |
| identification and expression analysis of two developmentally regulated myosin heavy chain gene transcripts in carp (cyprinus carpio). | whilst developmentally regulated genes for the myosin heavy chain (myohc) have been characterised in mammalian, avian and amphibian species, no developmental myohc gene has previously been characterised in a species of fish. in this study, we identify two developmentally regulated myohc gene transcripts (named eggs22 and eggs24) in carp (cyprinus carpio) and characterise their expression patterns during embryonic and larval development. the transcripts showed an identical temporal pattern of exp ... | 1999 | 10101107 |
| extracellular levels of amino acid neurotransmitters during anoxia and forced energy deficiency in crucian carp brain. | the crucian carp is one of the few vertebrates that has the ability to survive long periods of anoxia. a devastating event in the anoxic mammalian brain is a massive release of excitatory neurotransmitters, particularly glutamate. using microdialysis to measure extracellular levels of several amino acid neurotransmitters and related compounds in the telencephalon of crucian carp in vivo, we show here that this species avoids a release of glutamate during anoxia, which is probably related to its ... | 1999 | 10095011 |
| zinc-induced damage to carp (cyprinus carpio l.) erythrocytes in vitro. | fish erythrocytes were used to elucidate the effect of zinc ions on the cell antioxidant defence system. it was detected that an increase of the zn2+ concentration (0.01-1 mm) leads to a marked decrease (p < 0.05) in the catalase and the glutathione peroxidase activities. we observed a loss of 14-39% activity of glutathione peroxidase, and 16-20% diminution for catalase. no significant changes were found in case of the superoxide dismutase. incubation of red blood cells with zinc brought about a ... | 1999 | 10092948 |
| characterization of the carp myosin heavy chain multigene family. | we isolated partial coding sequences for 29 carp myosin heavy chain genes (myohcs) and determined the nucleotide sequences around the region encoding the loop 2 of the myosin molecule. the predicted amino acid sequences from the isolated genes all showed very high similarity to those of skeletal and cardiac muscles from higher vertebrates, but not to those of smooth and non-muscle counterparts. among all clones isolated, carp myohc10, myohci-1-3 and myohc30 showed exon-nucleotide sequences ident ... | 1999 | 10072772 |
| lethal effects of mexel 432, an antifouling agent, on embryolarval development of common carp (cyprinus carpio l.). | embryolarval lethal effects of mexel 432, an antifouling agent, were determined in the laboratory during continuous exposure, beginning at fertilization, under various experimental conditions in eight different spawns of the common carp (cyprinus carpio l). in synthetic water at 24 degrees c and without larval feeding, correlations between lc50 values and the various experimental conditions tested, except mexel 432 concentration, were low. the mean lc50 values were 1.13+/-0.35 mg/liter after 3 d ... | 1999 | 10051358 |
| zinc modulation of ampa receptors may be relevant to splice variants in carp retina. | with the use of the whole-cell patch clamp technique, we examined effects of zinc on ampa receptors of isolated carp retinal horizontal cells, predominantly consisting of flop splice variants. we found that zinc ranging from 30 microm to 1 mm failed to modulate glutamate-induced currents of these cells, which is clearly distinct from the results previously obtained in superior colliculus neurons and xenopus ooctyes. furthermore, glutamate responses remained unchanged when zinc was co-applied wit ... | 1999 | 10025587 |
| body bending during fast-starts in fish can be explained in terms of muscle torque and hydrodynamic resistance | fish fast-starts are rapid events caused by the simultaneous onset of muscle activity along one side of the body. spine curvature and the strain and electromyographic activity in white muscle were measured for fast-starts in the common carp cyprinus carpio. the first bend of the fast-start was powered by muscle on the concave side: muscle fibres on this side were activated and began shortening simultaneously between the length-specific longitudinal sites 0.3l and 0.56l, where l is total body len ... | 1999 | 10021321 |
| estimated intakes of trans fatty and other fatty acids in the us population. | to estimate mean level of trans fatty acid intakes using a representative sample of the us population. | 1999 | 9972183 |
| sublethal effects of phenol on the phospholipid fatty acid composition of carp erythrocyte plasma membrane. | the effect of different concentrations (5, 10, and 20 ppm) of phenol on the carp erythrocyte plasma membrane was examined following exposures of 48 and 96 h. the results indicated that the high concentrations of phenol pollutant led to an increase in phosphatidylcholine (pc) and eliminated phosphatidylinositol (pi), phosphatidylserine (ps), and phosphatidic acid (pa). the data also revealed that arachidonic acid (20:4 n-6) was present in the greatest amounts; its quantity in both low and high do ... | 1999 | 9931236 |
| white muscle strain in the common carp and red to white muscle gearing ratios in fish | white muscle strains were recorded using sonomicrometry techniques for 70 fast-starts in the common carp cyprinus carpio l. high-speed cine images were recorded simultaneously for 54 of these starts, and muscle strain was calculated independently from the digitized outlines of the fish. sonomicrometry measurements of superficial muscle strain were not significantly different from the strain as calculated from the theory of simple bending of a homogeneous material: superficial muscle strain thus ... | 1999 | 9929455 |
| the impact of rice pesticides on the aquatic ecosystems of the sacramento river and delta (california). | since the early 1980s, when molinate was demonstrated to have killed carp in agricultural drains, an intensive research effort has been undertaken to assess the impact of rice pesticides on aquatic ecosystems in the sacramento river and delta. no impact has been found that can be clearly attributed to rice pesticides. however, the rice insecticides methyl parathion and carbofuran, and probably also bufencarb, reached levels in the river and delta that, based on laboratory bioassays, would have b ... | 1999 | 9921140 |
| zn2+ differentially modulates glycine receptors versus gaba receptors in isolated carp retinal third-order neurons. | it was previously reported that strychnine-sensitive glycine receptors and gabaa receptors co-existed on carp retinal third-order neurons (amacrine/ganglion cells) (li, p. and yang, x.-l., strong synergism between gabaa and glycine receptors on isolated carp third-order neurons. neuroreport, 9 (1998) 2785-2789.). in this study, the effects of the divalent cation zn2+ on these two receptors were studied in amacrine/ganglion cells acutely isolated from carp, with the use of the whole-cell patch cl ... | 1999 | 10430508 |
| a 30-residue fragment of the carp granulin-1 protein folds into a stack of two beta-hairpins similar to that found in the native protein. | upon air oxidation, a peptide corresponding to the 30-residue n-terminal subdomain of carp granulin-1 spontaneously formed the disulfide pairing observed in the native protein. structural characterization using nmr showed the presence of a defined secondary structure within this peptide. the chemical shifts for most of the alphach protons of the peptide and the protein are very similar, and the observed noe contacts of the peptide strongly resemble those in the protein. a structure calculation o ... | 1999 | 10424355 |
| comparative investigation on spindle behavior and mpf activity changes during oocyte maturation between gynogenetic and amphimictic crucian carp. | the spindle behavior and mpf activity changes in the progression of oocyte maturation were investigated and compared with cytological observation and kinase assay between gynogenetic silver crucian carp and amphimictic colored crucian carp. mpf activity was measured by using histone h1 as phosphorylation substrate. there were two similar oscillatory mpf kinase activity changes during oocyte maturation in two kinds of fishes with different reproductive modes, but there existed some subtle differe ... | 1999 | 10418734 |
| trk-neurotrophin receptor-like immunoreactivity in the gut of teleost species. | neurotrophins, acting through their high-affinity signal-transducing trk receptors, are involved in the development, differentiation and maintenance of discrete neuron populations in the higher vertebrates. furthermore, the presence of trk receptors in some non-neuronal tissues, including the endocrine cells of the gut, could indicate an involvement of neurotrophins also in these tissues. recently, neurotrophins and neurotrophin receptor proteins have been identified in the lower vertebrates and ... | 1999 | 10408916 |
| ultrastructural alterations in the liver and intestine of carp cyprinus carpio induced orally by ultra-low doses of endosulfan. | in order to elucidate the importance of food-borne chemical contamination in fish, cytological and ultrastructural alterations in hepatocytes and enterocytes of common carp cyprinus carpio l. exposed for 5 wk to 0.5 microgram endosulfan (6,7,8,9,10,10-hexachloro-1,5,5a,6,9a-hexahydro-6,9-methano-2,4,3-benzo- dioxyanthiepin-3-oxide) kg-1 food dry weight, equivalent to an ultra-low dosis of 15 ng kg-1 fish d-1, were investigated by means of light and electron microscopy. observations on liver alte ... | 1999 | 10401584 |
| characterization of two zebrafish cdna clones encoding egg envelope proteins zp2 and zp3. | two zebrafish cdna clones encoding homologs of mammalian zona pellucida proteins zp2 and zp3 were isolated from a whole adult cdna library. the zp2 clone encodes a protein of 428 amino acids. unlike other teleost zp2s that contain an n-terminal repetitive domain enriched with prolines and glutamines, the zebrafish zp2 has no such repetitive domain. in the c-terminal non-repetitive domain, the zebrafish zp2 shares 55-76% sequence identity with other teleost zp2s. the zp3 cdna clone encodes a prot ... | 1999 | 10395930 |
| a model for circular dichroism monitored dimerization and calcium binding in an ef-hand synthetic peptide. | ef-hand peptides have been shown to bind calcium and dimerize to form an intact protein domain [shaw, g.s., hodges, r.s. & sykes, b.d. (1990). science, 249, 280-283]. a synthetic 33-residue ef-hand peptide with the sequence of carp parvalbumin cd site demonstrated a seven-fold increase in the apparent calcium dissociation constant with a eight-fold decrease in peptide concentration when fit to a single-site calcium-binding model. this observation is consistent with ef-hand dimerization. this pap ... | 1999 | 10395814 |
| effects of trifluralin on carp: biochemical and histological evaluation. | acute and subacute toxicity of the herbicide trifluralin on fish was investigated in laboratory toxicity tests with carp. median lethal concentrations were determined in acute tests. the 96-h lc50 value was 0.045 (0.036-0.051)mg/l. fish were exposed to subacute concentrations of the herbicide (0.005, 0.01, and 0.02 mg/l trifluralin) in the 14-day toxicity tests and the effects on the relative growth rate, some biochemical parameters [alkaline phosphatase (alp), aspartate aminotransferase (ast), ... | 1999 | 10375424 |
| zebrafish cth1, a c3h zinc finger protein, is expressed in ovarian oocytes and embryos. | the zfcth1 gene is, as the previously cloned carp cth1 gene, related to the mammalian tis 11 family of primary response genes and encodes a protein with two putative ccch zinc fingers. this report describes the rna expression of this gene during oogenesis and early embryogenesis up to gastrulation in the zebrafish (danio rerio). maternal cth1 message is present in the ovary of 1-month-old fish and of adult fish in oocytes at all stages of maturation. in the youngest oocytes the message is locali ... | 1999 | 10370129 |
| size limitations in semicircular duct systems | the present article discusses mechanical requirements and limitations which are applicable to the construction of the system of semicircular ducts, especially to its size. the simplified case of a single, uniform duct system has been considered which can be described by a second order equation of motion. the principal functional quantities for this rotation-sensor are: (1) response speed; (2) sensitivity; and (3) regular flow. the response speed of a single, uniform semicircular duct is characte ... | 1999 | 10366494 |
| recovery of acetylcholinesterase activity in the common carp (cyprinus carpio l.) after inhibition by organophosphate and carbamate compounds. | 1999 | 10353999 | |
| kinetics of cadmium accumulation and elimination in carp cyprinus carpio tissues. | carp (cyprinus carpio) were tested for cadmium accumulation and elimination during and after a simulated pollution exposure. fish were distributed in two 1000-l indoor concrete aquaria supplied with a continuous flow (8 l min(-1)) of well water. the cadmium concentration was maintained at 53 microg l(-1) in one aquarium and 443 microg l(-1) in the other aquarium for 127 days. the exposure phase was followed by a 43-day depuration period. the cadmium accumulation in liver, kidney and muscle was m ... | 1999 | 10336095 |
| in vitro vitellogenin production by carp (cyprinus carpio) hepatocytes as a screening method for determining (anti)estrogenic activity of xenobiotics. | the yolk protein precursor vitellogenin (vtg) is secreted by the liver of female as well as male fish, in response to estrogenic compounds. in this study, an in vitro assay was developed for measuring vtg induction, using cultured primary hepatocytes from genetically uniform strains of carp (cyprinus carpio). vtg production was measured by indirect competitive elisa, using a polyclonal antiserum against goldfish vtg that cross-reacts with carp vtg. vtg was dose-dependently induced by 17beta-estr ... | 1999 | 10329509 |
| pore-forming properties and antibacterial activity of proteins extracted from epidermal mucus of fish. | among several biological functions, the epidermal mucus of fish may play an important role in host defense, particularly in the prevention of colonization by parasites, bacteria and fungi. in previous work, two hydrophobic proteins of 27 and 31 kda were isolated from carp mucus. this study identified a strong antibacterial activity (0.16-0.18 microm) well correlated with pore-forming properties. here this work was extended to other fish species, four fresh water fish and one sea water fish. afte ... | 1999 | 10327617 |
| direct electrospray-ionization mass spectrometric analysis of the major ganglioside from crucian carp liver after thin layer chromatography. | ganglioside patterns from crucian carp brain, muscle, and liver as well as liver gangliosides of roach, carp, the cichlid oreochromis mossambicus, pigeon, dwarf hamster, and calf were comparatively analyzed by high performance thin layer chromatography (hptlc). to achieve a rapid estimation on potentially interesting ganglioside compounds, electrospray-ionization mass spectrometry (ms) was directly applied to a chloroform/methanol extract of the major tlc band of crucian carp liver. the spectrum ... | 1999 | 10327597 |
| frontonasal dysplasia, macroblepharon, eyelid colobomas, ear anomalies, macrostomia, mental retardation, and cns structural anomalies. a new syndrome? | we report a brazilian girl, born to normal and non-consanguineous parents and presenting, among other signs, brachyacrocephaly, a wide forehead, hypertelorism, wide palpebral fissures with multiple eyelid colobomas, a broad and high nasal root, an absent nasal tip, a wide columella, a long and smooth philtrum, a carp-like mouth, macrostomia, a thin upper lip with midline notching, submucous cleft of the soft palate, a small and grooved chin, ear anomalies, dandy-walker anomaly, a structural anom ... | 1999 | 10327243 |
| cold-induced ependymin expression in zebrafish and carp brain: implications for cold acclimation. | cold acclimation has been suggested to be mediated by alternations in the gene expression pattern in the cold-adapted fish. to investigate the mechanism of cold acclimation in fish brain at the molecular level, relevant subsets of differentially expressed genes of interest were identified and cloned by the pcr-based subtraction suppression hybridization. characterization of the selected cold-induced cdna clones revealed one encoding ependymin. this gene was shown to be brain-specific. the expres ... | 1999 | 10508924 |
| impact of mixture of nickel and chromium on the protein content of flesh and liver of cyprinus carpio during spawning and post-spawning phases. | 1999 | 10501728 | |
| impact of moderate silver carp biomass gradient on zooplankton communities in a eutrophic reservoir. consequences for the use of silver carp in biomanipulation. | we examined the impacts of moderate gradient silver carp biomass (five levels from 0 to 36 g.m-3, i.e. about 0-792 kg.ha-1) on zooplankton communities of the eutrophic villerest reservoir (france). during our mesocosm experiment changes in zooplankton assemblages were dependent on silver carp biomass. in the fishless and low fish biomass treatments, zooplankton abundance increased through time, owing to a peak in cladoceran density, but decreased (mainly cladocerans) at highest fish biomass. cop ... | 1999 | 10488436 |
| tissue-specific changes in rna synthesis in vivo during anoxia in crucian carp. | the overall energy budget for protein synthesis (i.e., transcription plus translation) is thought to consist of fixed and variable components, with rna synthesis accounting for the former and protein synthesis the latter. during anoxia, the downregulation of protein synthesis (i.e., the variable component), to reduce energetic demand, is an important aspect of survival in crucian carp. the present study examines rna synthesis during anoxia by labeling with [(3)h]uridine. a novel synthesis rate c ... | 1999 | 10484485 |
| rodlet cells in the head and trunk kidney of the domestic carp (cyprinus carpio): enigmatic gland cells or coccidian parasites? | rodlet cells have been found in the head and trunk kidneys of the common carp (cyprinus carpio l). from an experimental sample of 50 carps of various ages, we detected these cells in only seven fishes, contradicting the hypothesis that they constitute a normal component of the fish epithelia. the rodlet cells have a typical structure: 12-16 microns in diameter, with a basal nucleus various in form, and an encasing layer of fibrillar structure. the cells contain rodlets, composed of elongated, op ... | 1999 | 10481827 |
| estrogenic potencies of several environmental pollutants, as determined by vitellogenin induction in a carp hepatocyte assay. | estrogenic potencies of several xenoestrogens were determined in vitro, using cultured hepatocytes from a genetically uniform male carp strain (cyprinus carpio). estrogenicity was measured as induction of the yolk protein precursor vitellogenin (vtg), and compared to vtg induction by 17beta-estradiol (e2). the order of estrogenic potency was: methoxychlor (mxcl) > o,p-ddt > chlordecone approximately/= bisphenol-a approximately/= 4-t-pentylphenol. estrogenic potencies of these compounds varied fr ... | 1999 | 10478856 |
| purification, characterization, and molecular cloning of an outer layer protein of carp fertilization envelope. | an outer layer protein of carp fertilization envelope (fe), feo-1, was purified from carp oocytes. the cdnas encoding feo-1 were cloned. the mature protein of feo-1 is 21 kda in molecular weight and contains 177 amino acid residues whose sequence has 58% identity to the outer layer protein of chick vitelline membrane. in situ hybridization and immunocytochemistry show that feo-1 is expressed in oocytes and liver. in oocytes, feo-1 is stored in the cortical granules. during cortical reaction, it ... | 1999 | 10471479 |
| radionuclides and trace elements in fish collected upstream and downstream of los alamos national laboratory and the doses to humans from the consumption of muscle and bone. | the purpose of this study was to determine radionuclide and trace element concentrations in bottom-feeding fish (catfish, carp, and suckers) collected from the confluences of some of the major canyons that cross los alamos national laboratory (lanl) lands with the rio grande (rg) and the potential radiological doses from the ingestion of these fish. samples of muscle and bone (and viscera in some cases) were analyzed for 3h, 90sr, 137cs, totu, 238pu, 239,240pu, and 241am and ag, as, ba, be, cr, ... | 1999 | 10466108 |
| effects of synthetic pyrethroids and methidation on activities of some digestive enzymes in carp (cyprinus carpio l.). | the effects of pyrethroid pesticides (deltamethrin, permethrin and cypermethrin) and an organophosphate ester (methidation) on the activities of carp trypsin, alpha-chymotrypsin, carboxypeptidase a and lipase were studied. the enzymes were isolated from the gastrointestinal tract and the effects of the pesticides were investigated during incubation for 5 min. the activity of trypsin was influenced only slightly by the presence of deltamethrin and methidation, whereas permethrin and cypermethrin ... | 1999 | 10466103 |
| simultaneous acute renal and hepatic failure after ingesting raw carp gall bladder. | 1999 | 10462287 | |
| changes in the odorants of boiled carp fillet (cyprinus carpio l.) as affected by increasing methionine levels in feed. | sixty common carp in groups of five in four tanks per treatment were given three diets containing different increasing amounts of methionine. the aroma extract dilution analysis (aeda) of the boiled carp fillets resulted in 32 odorants, of which 26 were identified. ten compounds were quantified using an internal standard (is), and the very low concentrations of 2-acetyl-1-pyrroline, (z)-1, 5-octadien-3-one, and methional were calculated by the nasally determined detection limit. the concentratio ... | 1999 | 10606586 |
| [reaction to purchase of infected koi carp]. | 1999 | 10596402 | |
| relationship between the structure of some humic compounds and their inhibitory effects on carp catalase. | 1999 | 10594149 | |
| the biomechanics of fast-starts during ontogeny in the common carp cyprinus carpio | common carp cyprinus carpio l. were reared a constant temperature of 20 degrees c from the larval (7 mm total length) to the juvenile (80 mm) stage. body morphology and white muscle mass distribution were measured. fast-start escape responses were recorded using high-speed cinematography from which the velocities, accelerations and hydrodynamic power requirements were estimated. all three measures of fast-start performance increased during development. white muscle contraction regimes were calcu ... | 1999 | 10539954 |
| in vivo bafilomycin-sensitive na(+) uptake in young freshwater fish | in vivo treatment with external bafilomycin a(1), a selective inhibitor of v-atpase h(+) pumps, reduced whole-body na(+) influx by up to 90 % in young tilapia and 70 % in young carp. the inhibition was rapidly reversible, with whole-body na(+) influx rebounding to 280 % of pre-treatment values within 20 min of removal from the bafilomycin. this rebound effect is consistent with the prior accumulation of protons during the period when the cells were exposed to bafilomycin. bafilomycin also inhibi ... | 1999 | 10574743 |
| zn2+ differentially modulates kinetics of gaba(c) vs gaba(a) receptors in carp retinal bipolar cells. | gaba(c) and gaba(a) receptors co-exist in retinal bipolar cells. in the present study the effects of zinc on the kinetics of currents mediated by gaba(c) and gaba(a) receptors were investigated in isolated carp bipolar cells, using whole-cell patch-clamp technique. we observed for the first time that zinc exerted opposite effects on kinetics of the gaba(c) and gaba(a) responses: zinc significantly slowed down activation and desensitization of the gaba(c) response, but accelerated those of the ga ... | 1999 | 10574375 |
| transcription factor pit-1 expression is modulated upon seasonal acclimatization of eurythermal ectotherms: identification of two pit-1 genes in the carp. | a second pit-1 gene in carp (cyprinus carpio), including the complete structural gene and 1.1 kb of promoter region, was identified and completely sequenced. the exon-intron structure was determined, and reverse transcription-polymerase chain reaction (rt-pcr) experiments suggest that only one pit-1 splice variant is present in carp pituitary. the effect of seasonal acclimatization on the extent of pit-1 gene expression was studied in summer- and winter-acclimatized carp. quantitative rt-pcr ana ... | 1999 | 10572243 |
| effect of zinc on carp (cyprinus carpio l.) erythrocytes. | the effect of zinc exposure on some properties of the carp erythrocyte membrane was studied in vitro. red blood cells plasma membranes were separated from other cellular membranes using a combination of differential and density gradient centrifugation. the purity of obtained plasma membrane preparations was determined by measuring the activity of the marker enzymes. electrophoretic patterns of the main erythrocyte membrane proteins excluded their degradation during the isolation and purification ... | 1999 | 10530892 |
| uncoupling protein 2 from carp and zebrafish, ectothermic vertebrates. | uncoupling protein 1 (ucp1) is of demonstrated importance in mammalian thermogenesis, and early hypotheses regarding the functions of the newly discovered ucp homologues, ucp2, ucp3 and others, have focused largely on their potential roles in thermogenesis. here we report the amino acid sequences of two new ucps from ectothermic vertebrates. ucps from two fish species, the zebrafish (danio rerio) and carp (cyprinus carpio), were identified in expressed sequence tag databases at the european mole ... | 1999 | 10524261 |
| effect of insulin-like growth factor-1 on steroidogenesis in cultured carp ovarian follicles: interactions with estradiol. | the biological action of insulin like growth factor-1 (igf-1) on follicular steroidogenesis during follicular development in common carp was examined. studies were carried out by culturing small (1-2 mm diam.) and large (> 2 mm diam.) follicles. igf-1 (0.3-100 ng/ml) had no effect on progesterone accumulation or aromatase activity during 48 hr culture of small follicles. progesterone accumulation by large follicles was also unaffected by igf-1 over the same period, although aromatase activity wa ... | 1999 | 10641132 |
| mortality of fish fry as a result of specific and aspecific cercarial invasion under experimental conditions. | although the effect exerted on fish by fluke cercariae developing in fish as intermediate hosts is fairly well known, cercariae of flukes requiring intermediate hosts other than fish occur in fish ponds in much higher numbers. to study the effect of these latter cercariae on fish fry, a few days old common carp fry (cyprinus carpio) were exposed to cohorts of xiphidiocercariae or echinocercariae shed by one or two snails acting as intermediate hosts. the observed pathogenicity of these cercariae ... | 1999 | 10641334 |
| caffeine-sensitive ca2+ stores in carp retinal bipolar cells. | high k+- or caffeine-induced ca2+ signal was studied in freshly dissociated carp retinal on-type bipolar cells using a confocal laser-scanning microscope. in response to 35 mm k+ exposure, a rise in [ca2+]i appeared in both the terminal and soma, but was absent after removal of external ca2+ or in the presence of 100 microm nifedipine. it is indicated that, for high k+-induced increase of [ca2+]i, ca2+ influx through voltage-gated l-type ca2+ channels is essential and ca2+ entry through reversed ... | 1999 | 10716230 |
| nasda aquatic animal experiment facilities for space shuttle. | national space development agency of japan (nasda) has been developed aquatic animal experiment facilities for space experiments using nasa space shuttle. vestibular function experiment unit (vfeu) has been firstly designed and developed for spacelab-j mission (sts-47), and 8 days space experiment with carp has been performed. following, the vfeu, aquatic animal experiment unit (aaeu) has been developed to accommodate small aquatic animals second international microgravity laboratory mission (im ... | 1999 | 11542796 |
| hematology and clinical chemistry of cyprinid fish. common carp and goldfish. | evaluation of the clinical status in aquatic species is compromised by the limited diagnostic techniques that can be performed in these species. the hematologic and plasma chemistry parameters can provide predictive information, although these parameters can be highly variable owing to the influence of various intrinsic and extrinsic factors. however, these parameters are fairly stable in acclimated, well-managed fish if stress is reduced during collection and samples are properly collected and ... | 1999 | 11229053 |
| [glycine modulates the erg b-waves and on-type bipolar cells in carp retina]. | in the present work we investigated the effects of glycine on the electroretinograms (erg) and light responses of on-type bipolar cells under dark- and light-adapted conditions in superfused, isolated crucian carp retinas. it was revealed that application of 4 mmol/l glycine significantly suppressed the b-waves and the responses of on-type bipolar cells and the effects were blocked by co-application of 20 mumol/l strychnine. in addition, glycine had no apparent action on the piii component (phot ... | 1999 | 11499004 |
| [light and electron microscopic observation of zinc distribution in the carp retina]. | localization of zinc in the carp retina in both light and dark adaptation was investigated using neo-timm sulfate silver method. the results showed that in light adaptation, zinc was preponderantly present in the outer plexiform layer (opl). both terminals of zinc containing photoreceptors and dendrites of bipolar cells usually extend into zinc staining areas in opl. in dark adaptation, zinc staining in retina is blanched or only appeared in cytoplasmic bodies of few cones. there is no significa ... | 1999 | 11498989 |
| interaction of cone signals on l-type horizontal cell in carp retina: experiments and modeling. | intracellular recordings were made from the luminosity type cone driven horizontal cells (lhcs) in the isolated carp retina and model analysis was performed to investigate possible mechanisms underlying the interaction of different cone signals converging onto these cells. it was observed that a green background light enhanced the lhc's red response, and such enhancement was closely related to the activation of green cones. model analysis showed that the activity of both glutamate- and gaba-rela ... | 1999 | 11498964 |
| comparative study of acetylcholinesterase and butyrylcholinesterase in brain and serum of several freshwater fish: specific activities and in vitro inhibition by ddvp, an organophosphorus pesticide. | a comparative study of specific activities and in vitro inhibition of brain and serum acetylcholinesterase (ache; ec 3.1.1.7) and serum butyrylcholinesterase (bche; ec 3.1.1.8) by ddvp, an organophosphorus pesticide, was conducted in 11 freshwater teleost species belonging to four families (cyprinidae; common carp cyprinus carpio, bream abramis brama, blue bream a. ballerus, white bream blicca bjoerkna, roach rutilus rutilus, bleak alburnus alburnus, ide leuciscus idus; percidae: perch perca flu ... | 2000 | 11246494 |
| effect of the pesticide deltamethrin on the mauthner cells of lake balaton fish. | deltamethrin, a synthetic pesticide [(s)alpha-cyano-3-phenoxybenzyl-(1r)-cis-3-(2.2-dibromovinyl)-2,2-dim ethylcyclopropane-carboxylate] used for extermination of mosquitoes on the shores of lake balaton, has been found to induce severe impairments of the nervous system of several lake balaton fish, such as carp (cyprinus carpio), goldfish (carassius auratus gibelis bloch), eel (anguilla anguilla) and wels (silurus glanis). it has been shown that deltamethrin, in a concentration of 1 microgram/l ... | 2000 | 10894124 |
| organochlorine residues in european eel (anguilla anguilla), crucian carp (carassius carassius) and catfish (ictalurus nebulosus) from vaccarès lagoon (french national nature reserve of camargue) - effects on some physiological parameters. | european eels (anguilla anguilla), crucian carps (carassius carassius) and catfish (ictalurus nebulosus) were collected in three coastal locations of the vaccarès lagoon (french national nature reserve of camargue). the purpose of this paper is to report results of the first biomonitoring investigation in fish living in this protected coastal wetland. residues of organochlorine (oc) contaminants (i.e. sigmapcbs, gammahch, hcb, dieldrin, pp'-dde) were determined in hepatic and muscular tissues, i ... | 2000 | 10794830 |
| horseradish peroxidase binding to intestinal brush-border membranes of cyprinus carpio. identification of a putative receptor. | morphologic studies have shown that the classic endocytosis tracer horseradish peroxidase (hrp) is actively internalized by vesicular transport in the carp intestine, suggesting the existence of specific binding sites in the apical membrane of enterocytes. the aim of the present study was to develop an in vitro binding assay using isolated carp intestinal brush-border membranes (bbm) to demonstrate and characterize these specific hrp binding sites. the results obtained show that hrp binding to b ... | 2000 | 11074599 |
| preparation of neoglycoprotein from carp myofibrillar protein and alginate oligosaccharide: improved solubility in low ionic strength medium. | alginate oligosaccharide (ao) was conjugated with carp myofibrillar protein (mf) by using the controlled maillard reaction, and the change in the solubility of mf in low ionic strength media as affected by the glycosylation was investigated. ao was prepared by degrading sodium alginate using alginate lyase, which was purified from the culture supernatant of pseudoalteromonas elyakovii. when a lyophilized mf and ao mixture was incubated at 40 degrees c and 65% relative humidity, the conjugation o ... | 2000 | 10637043 |
| urocortin is the principal ligand for the corticotrophin-releasing factor binding protein in the ovine brain with no evidence for a sauvagine-like peptide. | to purify novel ligands for the corticotrophin-releasing factor binding protein (crf-bp) from ovine brain, whole brain was homogenised in methanol and the supernatant extracted on sep-pak c18 cartridges followed by a preliminary hplc step. three peaks of ovine crf-bp ligand activity were detected in the hplc fractions, the first two of which were also detected by a specific corticotrophin-releasing factor two-site immunoradiometric assay, the third peak being detected by a human crf-bp ligand as ... | 2000 | 10656997 |
| chronic toxic effects of quinalphos on some biochemical parameters in labeo rohita (ham.). | the effect of exposure to sublethal concentrations of the organophosphate pesticide, quinalphos (1.12, 0.22 mg/l) on biochemical parameters of muscle and enzyme activities in brain, liver and kidney of the indian major carp, labeo rohita was studied after 15, 30 and 45 days. the muscle protein and rna levels decreased whereas dna levels and acid phosphatase were elevated. similarly, alkaline phosphatase was depleted. the brain acetyl cholinesterase activity was decreased most (-75.43%) in 1.12 m ... | 2000 | 10713464 |
| design and solution structure of a well-folded stack of two beta-hairpins based on the amino-terminal fragment of human granulin a. | four amino acid substitutions were introduced into a peptide corresponding to the amino-terminal subdomain (30-31 residues) of human granulin a (hga) in order to assess the contributions of a hydrophobic framework and other interactions to structure stabilization of the stack of two beta-hairpins. the resulting hybrid peptide, hga 1-31 (d1v, k3h, s9i, q20p) with four free cysteines, spontaneously formed a uniquely disulfide-bonded isomer with an expected [1-3, 2-4] disulfide pairing pattern. thi ... | 2000 | 10715107 |
| pathological and biochemical characterization of microcystin-induced hepatopancreas and kidney damage in carp (cyprinus carpio). | mass occurrences of cyanobacteria, due to their inherent capacity for toxin production, specifically of microcystins (mc), have been associated with fish kills worldwide. the uptake of mc-lr and the sequence of pathological and associated biochemical changes was investigated in carp (cyprinus carpio) in vivo over 72 h. carp were gavaged with a single sublethal bolus dose of toxic microcystis aeruginosa (pcc 7806) amounting to an equivalent of 400 microg mc-lr/kg body wt. damage of renal proximal ... | 2000 | 10739746 |
| surface ultrastructure of metagonimus takahashii metacercariae and adults. | a scanning electron microscopic study was performed on the surface ultrastructure of metacercariae and adults of metagonimus takahashii. metacercariae were collected from the scale of crucian carp (carassius auratus), and adult flukes were harvested 1-4 weeks after infection to rats. in excysted metacercariae, the oral sucker had type i (numerous) and type ii (seven in total) sensory papillae. tegumental spines were dense and digitated into 5-7 points on the surface anterior to the ventral sucke ... | 2000 | 10743353 |
| a novel truncated isoform of the mannose-binding lectin-associated serine protease (masp) from the common carp (cyprinus carpio). | the mannose-binding lectin (mbl) and mbl-associated serine proteases (masps) play crucial roles in activation of the lectin pathway of the complement system. mammals and xenopus possess two distinct masps, masp1 and masp2, with different substrate specificity. recently, a truncated form named map19 or smap, composed of n-terminal c1r/c1s/uegf/bone morphogenetic protein (cub)-1 and epidermal growth factor domains of masp2, has been shown to be generated by alternative polyadenylation and splicing ... | 2000 | 10752628 |
| gonadotropin-releasing hormone stimulates thyroid activity in a freshwater murrel, channa gachua (ham.), and carps, catla catla (ham.) and cirrhinus mrigala (ham.). | injections of mammalian gnrh (mgnrh), salmon gnrh (sgnrh), and a homologous murrel, channa punctatus, gnrh (cgnrh) to a murrel, channa gachua, and a carp, catla catla, at a dose of 1 microg/250 g body wt significantly increased plasma thyroxine (t4) levels above control. piscine gnrhs (sgnrh and cgnrh) had significantly greater stimulatory effects compared with mgnrh. to observe whether this stimulatory effect by gnrhs is direct or indirect, thyroid follicles were isolated from hypobranchial mus ... | 2000 | 10764556 |
| viremia-associated ana-aki-byo, a new viral disease in color carp cyprinus carpio in japan. | a new virus disease that displays dermal ulceration and high mortality has been occurring since 1996 in color carp cyprinus carpio reared in warm water in japan. in histological examinations, initial erosive lesions displayed necrosis, hemorrhage and fibrin deposition in the dermal loose connective tissue and were accompanied by the partial destruction of the epidermis. developed ulcerative lesions involved the lateral musculature with bacterial invasions. in visceral organs, necrotic cells were ... | 2000 | 10768286 |
| a study of sequential histopathology of trypanoplasma borreli (protozoa: kinetoplastida) in susceptible common carp cyprinus carpio. | the tissue response of common carp cyprinus carpio to the kinetoplastid blood parasite trypanoplasma borreli laveran & mesnil, 1901 was investigated during a laboratory infection of a highly susceptible carp line. with the development of the parasitaemia an increased proliferation of the lymphoid renal interstitial tissue was induced, which resulted in a progressive depression and deterioration of renal tubules. in heavily infected carp at days 20 to 28 post inoculation (pi), a tubulonephrosis, ... | 2000 | 10768290 |
| up-regulation of natriuretic peptides in the ventricle of csx/nkx2-5 transgenic mice. | a cardiac homeobox-containing gene csx/nkx2-5, which is essential for cardiac development, is abundantly expressed in the adult heart as well as in the heart primordia. targeted disruption of this gene results in embryonic lethality due to abnormal heart morphogenesis. to elucidate the role of csx/nkx2-5 in the adult heart, we generated transgenic mice which overexpress human csx/nkx2-5. the transgene was expressed abundantly in the heart and the skeletal muscle. mrna levels of several cardiac g ... | 2000 | 10772952 |
| biochemical characterization of different types of adherence of vibrio species to fish epithelial cells. | vibrio species are gram-negative bacteria that cause a systemic infection in fish called vibriosis. the authors previously demonstrated that internalization and cytotoxicity are important virulence mechanisms in vibrio-fish epithelial cell interactions. adherence is a prerequisite for successful internalization. in this study, the adherence capability of two invasive strains [v. anguillarum 811218-5w and g/virus/5(3)] was compared with that of two non-invasive strains [v. damselae atcc 33539 and ... | 2000 | 10784057 |
| recombinant extracellular domain of rabbit growth hormone receptor and biological activity of somatogenic hormones. | the cdna of the extracellular domain of rabbit growth hormone receptor (rbghr-ecd) was cloned in the prokaryotic expression vector pmon, to enable its expression in escherichia coli after induction with nalidixic acid. the bacterially expressed rbprlr-ecd protein, contained within the refractile-body pellet, was solubilized in 4.5 m urea, refolded, and purified on a q-sepharose column, ph 8, by stepwise elution with nacl. the bioactive monomeric 28-kda fraction was eluted in 0.15 m nacl, yieldin ... | 2000 | 10794181 |
| molecular cloning of a carp jak3 from activated leukocytes. | janus kinases (jaks) are involved in a signalling pathway leading to tyrosine phosphorylation, which is the key step in transducing cytokine signals from the external environment to the nucleus. we report here the molecular cloning of carp jak3. a partial cdna of the jak homologue was initially identified from a cdna pool obtained by subtracting the cdnas from con a-induced carp head kidney leukocytes with those from uninduced carp head kidney leukocytes. the entire coding sequence was assembled ... | 2000 | 10802290 |
| molecular and functional characterization of a fish inducible-type nitric oxide synthase. | using an oligonucleotide primer based on a partial goldfish inducible nitric oxide synthase (inos) sequence, a complete carp inos cdna was isolated from an activated carp phagocyte cdna library. nucleotide and predicted amino acid sequence analysis indicate that carp inos encodes a 1,127-amino acid protein with 57% sequence identity to human inos. like mammalian noss, carp inos protein contains putative binding sites for heme, tetrahydrobiopterin, calmodulin, flavine mononucleotide, flavine aden ... | 2000 | 10803847 |
| specific cell-mediated cytotoxicity against a virus-infected syngeneic cell line in isogeneic ginbuna crucian carp. | in the present study, we attempted to determine whether virus-specific cell-mediated cytotoxicity occurs in fish. experiments were conducted with clonal ginbuna crucian carp (carassius auratus langsdorfii) (s3n strain), and the syngeneic cell line (cfs). two infectious viruses were used: infectious pancreatic necrosis virus (ipnv) and eel virus from america (eva). peripheral blood leukocytes, which consisted almost entirely (95%) of lymphocytes and thrombocytes, from s3n ginbuna immunized with v ... | 2000 | 10831797 |
| purification of a novel serine proteinase inhibitor from the skeletal muscle of white croaker (argyrosomus argentatus). | a novel serine proteinase inhibitor has been purified to homogeneity from the skeletal muscle of white croaker (argyrosomus argentatus). the purification was carried out by ammonium sulfate fractionation, deae-sephacel, heating treatment followed by column chromatographies on sp-sepharose, sephadex g-150 and gel-filtration high performance liquid chromatography. the molecular mass of the inhibitor was 55 kda as estimated by sds-page and gel filtration. it specifically inhibited a myofibril-bound ... | 2000 | 10833440 |
| properties of cytotoxic peptide-formed ion channels. | cytotoxic peptides are relatively small cationic molecules such as those found 1) in venoms, e.g., melittin in bee, scorpion toxins in scorpion, pilosulin 1 in jumper ant, and lycotoxin i and ii in wolf spider; 2) in skin secretions (e.g., magainin i and ii from xenopus laevis, dermaseptin from frog, antimicrobials from carp) and cells of the immune system (e.g., insect, scorpion, and mammalian defensins and cryptdins); 3) as autocytotoxicity peptides, e.g., amylin cytotoxic to pancreatic beta-c ... | 2000 | 10837335 |
| cytopathological observations on renal tubule epithelium cells in common carp cyprinus carpio under trypanoplasma borreli (protozoa: kinetoplastida) infection. | cytological alterations in renal tubule epithelium cells of carp cyprinus carpio infected with the blood flagellate trypanoplasma borreli laveran & mesnil, 1901 were investigated during the course of a laboratory infection of a highly susceptible carp line. with the development of the parasitaemia, a hyperplasia of the interstitial renal tissue was induced, which resulted in a tubulus necrosis. cytological changes were already seen in tubulus epithelium cells on day 7 post injection (pi) of the ... | 2000 | 10843558 |