Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| changes of anti-oxidative enzymes and mda content under soil water deficits among 10 wheat (triticum aestivum l.) genotypes at maturation stage. | drought is a world-spread problem seriously influencing grain production and quality, the loss of which is the total for other natural disasters, with increasing global climate change making the situation more serious. wheat is the staple food for more than 35% of world population, so wheat anti-drought physiology study is of importance to wheat production and biological breeding for the sake of coping with abiotic and biotic conditions. much research is involved in this hot topic, but the pace ... | 2005 | 16102947 |
| a qtl located on chromosome 4a associated with dormancy in white- and red-grained wheats of diverse origin. | improved resistance to preharvest sprouting in modern bread wheat (triticum aestivum. l.) can be achieved via the introgression of grain dormancy and would reduce both the incidence and severity of damage due to unfavourable weather at harvest. the dormancy phenotype is strongly influenced by environmental factors making selection difficult and time consuming and this trait an obvious candidate for marker assisted selection. a highly significant quantitative trait locus (qtl) associated with gra ... | 2005 | 16133305 |
| mapping of a major qtl for pre-harvest sprouting tolerance on chromosome 3a in bread wheat. | quantitative trait loci (qtl) analysis was conducted for pre-harvest sprouting tolerance (phst) in bread wheat for a solitary chromosome 3a, which was shown to be important for this trait in earlier studies. an inter-varietal mapping population in the form of recombinant inbred lines (rils) developed from a cross between spr8198 (a phs tolerant genotype) and hd2329 (a phs susceptible cultivar) was used for this purpose. the parents and the ril population were grown in six different environments ... | 2005 | 16133317 |
| genetic characterization and molecular mapping of a hessian fly-resistance gene transferred from t. turgidum ssp. dicoccum to common wheat. | a gene (temporarily designated hdic) conferring resistance to the hessian fly (hf) [mayetiola destructor (say)] was previously identified from an accession of german cultivated emmer wheat [triticum turgidum ssp. dicoccum (schrank ex schübler) thell] pi 94641, and was transferred to the hf-resistant wheat germplasm ks99wgrc42. the inheritance of hdic resistance exhibited incomplete penetrance because phenotypes of some heterozygous progenies are fully resistant and the others are fully susceptib ... | 2005 | 16136351 |
| evidence for the involvement of arabinoxylan and xylanases in refrigerated dough syruping. | the relationship between syruping in refrigerated doughs upon prolonged storage and different aspects of arabinoxylan (ax) hydrolysis was investigated using triticum aestivum xylanase inhibitor (taxi) and different xylanases in the dough formula. dough characteristics were evaluated with strong emphasis on the ax population and its fate as a function of storage time. selective reduction of part of the flour endogenous xylanase activity in dough by added taxi reduced dough syruping after 12 and 2 ... | 2005 | 16159195 |
| [identification of an alien chromosome in the common wheat line multi 6r]. | the chromosome of agropyron intermedium (host) beauv. substituting chromosome 6d has been identified in the karyotype of the triticum aestivum l. line multi 6r with the use of c-banding. the alien chromosome, temporarily designated 6ag1, contains gene(s) of the resistance to the saratov population of puccinia recondita rob. ex. desm. f. tritici. it has been demonstrated that the resistance gene(s) is(are) highly efficient and that chromosome 6ag1 is preferably transferred through gametes. | 2005 | 16161629 |
| mutations in wheat starch synthase ii genes and pcr-based selection of a sgp-1 null line. | wheat (triticum aestivum l.) starch synthase ii, which is also known as starch granule protein 1 (sgp-1), plays a major role in endosperm starch synthesis. the three sgp-1 proteins, sgp-a1, b1 and d1, are produced by three homoeologous ssii genes, wssii-a, b, and d. lines carrying null alleles for each sgp-1 protein have previously been identified. in this report, the mutations occurring in each wssii gene were characterized, and pcr-based dna markers capable of detecting the mutations were deve ... | 2005 | 16172895 |
| expression of transgenic stilbene synthases in wheat causes the accumulation of unknown stilbene derivatives with antifungal activity. | the expression of foreign phytoalexins in a new host is thought to increase fungal resistance, since host-specific pathogens have not experienced selection for detoxifying or metabolising the novel antifungal compounds. two resveratrol synthase genes vst1 and vst2 from grapevine (vitis vinifera l.) and the pinosylvin synthase gene pss from pine (pinus sylvestris l.) were stably transformed into bread wheat. the expression of the target genes is regulated by stress-inducible grapevine promoters. ... | 2005 | 16173460 |
| allelic series of four powdery mildew resistance genes at the pm3 locus in hexaploid bread wheat. | at the pm3 locus in hexaploid wheat (triticum aestivum), 10 alleles conferring race-specific resistance to powdery mildew (blumeria graminis f. sp. tritici) are known. a cluster of genes encoding coiled-coil-nucleotide-binding site-leucine-rich repeat proteins spans the pm3 locus on wheat chromosome 1a, and one member of this gene family has recently been identified as the pm3b resistance gene. using molecular markers closely linked to pm3b, we performed haplotype analysis of 10 lines carrying d ... | 2005 | 16183849 |
| localization of ptr toxa produced by pyrenophora tritici-repentis reveals protein import into wheat mesophyll cells. | the plant pathogenic fungus pyrenophora tritici-repentis secretes host-selective toxins (hsts) that function as pathogenicity factors. unlike most hsts that are products of enzymatic pathways, at least two toxins produced by p. tritici-repentis are proteins and, thus, products of single genes. sensitivity to these toxins in the host is conferred by a single gene for each toxin. to study the site of action of ptr toxa (toxa), toxin-sensitive and -insensitive wheat (triticum aestivum) cultivars we ... | 2005 | 16199615 |
| [activity of lectins of winter wheat seedlings infected with mycoplasma and treated with salicylic acid]. | changes in activity of soluble and cell wall lectins have been revealed in seedlings of winter wheat triticum aestivum l. cultivar mironovskaya 808 after infection with mycoplasma acholeplasma laidlawii 118. the protective effect of salicylic acid was manifested as negating the bursts of lectin activity induced by mycoplasma infection. | 2005 | 16212262 |
| characteristics of thiol:protein disulfide oxidoreductase from wheat (triticum aestivum l.) grain. | biochemical properties of a homogenous preparation of thiol:protein disulfide oxidoreductase (tpdo, ec 1.8.4.2) isolated for the first time from mature wheat (triticum aestivum l.) grain were studied. according to polyacrylamide gel electrophoresis data, the molecular weight of tpdo is around 167 kd, the enzyme consisting of two subunits of 77 and 73 kd, which differentiates tpdo from known enzymes of sh/ss-metabolism of wheat caryopses. in substrate specificity and enzymatic characteristics (ph ... | 2005 | 16212551 |
| structure of ptr toxa: an rgd-containing host-selective toxin from pyrenophora tritici-repentis. | tan spot of wheat (triticum aestivum), caused by the fungus pyrenophora tritici-repentis, has significant agricultural and economic impact. ptr toxa (toxa), the first discovered proteinaceous host-selective toxin, is produced by certain p. tritici-repentis races and is necessary and sufficient to cause cell death in sensitive wheat cultivars. we present here the high-resolution crystal structure of toxa in two different crystal forms, providing four independent views of the protein. toxa adopts ... | 2005 | 16214901 |
| classification of wheat varieties based on structural features of arabinoxylans as revealed by endoxylanase treatment of flour and grain. | arabinoxylans (ax) are cell wall polysaccharides of complex structure involved in many aspects of wheat flour end uses. the study of the variations of ax structure can lead to the identification of genes involved in their biosynthesis, and thus in the control of the various aspects of grain quality related to their presence. a method is proposed to identify ax variations directly in whole grain by enzymatic degradation. an endoxylanase from trichoderma viride was used to extract ax from a collec ... | 2005 | 16218687 |
| cloning, characterization and expression of wheat edr1 (enhanced disease resistance) gene. | to investigate if there is an edr1 pathway in wheat (triticum aestivum l.), a pair of degenerate primers was designed according to the cdnas of arabidopsis thaliana edr1 gene and its homologs were used to isolate edr1 gene homologs from wheat. rt-pcr was conducted on the cdna template synthesized with rna of wheat leaves. a 627-bp cdna fragment representing an edr1 gene (named as taedr1) was isolated (genbank accession number: ay743662). subsequently, the 3050-bp full-length cdna sequence of tae ... | 2005 | 16222089 |
| wheat leaf photosynthesis loss due to leaf rust, with respect to lesion development and leaf nitrogen status. | in wheat (triticum aestivum cv. soissons) plants grown under three different fertilisation treatments, we quantified the effect of leaf rust (puccinia triticina) on flag leaf photosynthesis during the whole sporulation period. bastiaans' model: y = (1 - x)beta was used to characterize the relationship between relative leaf photosynthesis (y) and disease severity (x). the evolution of the different types of symptoms induced by the pathogen (sporulating, chlorotic and necrosed tissues) was evaluat ... | 2005 | 15720636 |
| the application of bioassays as indicators of petroleum-contaminated soil remediation. | bioremediation has proven successful in numerous applications to petroleum contaminated soils. however, questions remain as to the efficiency of bioremediation in lowering long-term soil toxicity. in the present study, the bioassays spirotox, microtox, ostracodtoxkit f, umu-test with s-9 activation, and plant assays were applied, and compared to evaluate bioremediation processes in heavily petroleum contaminated soils. six higher plant species (secale cereale l., lactuca sativa l., zea mays l., ... | 2005 | 15722101 |
| induction of wheat defense and stress-related genes in response to fusarium graminearum. | fusarium head blight (fhb), caused by species of the fungus fusarium, is a worldwide disease of wheat (triticum aestivum l.). the chinese t. aestivum 'ning7840' is one of few wheat cultivars with resistance to fhb. to identify differentially expressed genes corresponding to fhb resistance, a cdna library was constructed using pooled mrna isolated from glumes of 'ning7840' harvested at 2, 6, 12, 24, 36, 72, and 96 h after inoculation (hai) with a conidia spore suspension of fusarium graminearum. ... | 2005 | 15729394 |
| mapping of genes expressed in fusarium graminearum-infected heads of wheat cultivar 'frontana'. | the isolation, physical, and genetic mapping of a group of wheat genes expressed in infected heads of triticum aestivum 'frontana' resistant to fusarium head blight is reported. a cdna library was built from heads of 'frontana' through suppressive subtractive hybridization, to enrich for sequences induced by the pathogen fusarium graminearum during infection. a group of 1794 clones was screened by dot blot hybridization for differential gene expression following infection. twenty of these clones ... | 2005 | 15729400 |
| inheritance and qtl analysis of durable resistance to stripe and leaf rusts in an australian cultivar, triticum aestivum 'cook'. | an f4-derived f6 recombinant inbred line population (n = 148) of a cross between the durable stripe (yellow) rust (caused by puccinia striiformis) and leaf (brown) rust (caused by puccinia triticina) resistant cultivar, triticum aestivum 'cook', and susceptible genotype avocet-yra was phenotyped at several locations in canada and mexico under artificial epidemics of leaf or stripe rusts and genotyped using amplified fragment length polymorphism (aflp) and microsatellite markers. durable adult pl ... | 2005 | 15729401 |
| manganese toxicity thresholds for restoration grass species. | manganese toxicity thresholds for restoration plants have not been established. as a result, ecological risk assessments rely on toxicity thresholds for agronomic species, which may differ from those of restoration species. our objective was to provide mn toxicity thresholds for grasses commonly used in restoration. we used a greenhouse screening study where seedlings of redtop, slender wheatgrass, tufted hairgrass, big bluegrass, basin wildrye, and common wheat were grown in sand culture and ex ... | 2005 | 15734591 |
| a novel dwarfing mutation in a green revolution gene from brassica rapa. | mutations in the biosynthesis or signaling pathways of gibberellin (ga) can cause dwarfing phenotypes in plants, and the use of such mutations in plant breeding was a major factor in the success of the green revolution. della proteins are ga signaling repressors whose functions are conserved in different plant species. recent studies show that ga promotes stem growth by causing degradation of della proteins via the ubiquitin-proteasome pathway. the most widely utilized dwarfing alleles in wheat ... | 2005 | 15734906 |
| first report of phoma sorghina (sacc.) boerema dorenbosch & van kest on wheat leaves (triticum aestivum l.) in argentina. | a new disease caused by phoma sorghina has been detected for the first time on wheat plants in the province of buenos aires, argentina. the pathogen was isolated from wheat leaves growing under field conditions, cultured on pda and identified by its morphobiometric and cultural characters. the disease symptoms and morphological characters of the pathogen are described. pathogenicity of the isolate was confirmed by inoculating 10 wheat cultivars under greenhouse conditions. | 2005 | 15750734 |
| wheat cytogenetics in the genomics era and its relevance to breeding. | hexaploid wheat is a species that has been subjected to most extensive cytogenetic studies. this has contributed to understanding the mechanism of the evolution of polyploids involving diploidization through genetic restriction of chromosome pairing to only homologous chromosomes. the availability of a variety of aneuploids and the ph mutants (ph1 and ph2) in bread wheat also allowed chromosome manipulations leading to the development of alien addition/substitution lines and the introgression of ... | 2005 | 15753592 |
| modeling carbon and nitrogen transformations for adjustment of compost application with nitrogen uptake by wheat. | environmentally sound management of the use of composts in agriculture relies on matching the rate of release of available n from compost-amended soils to the crop demand. to develop such management it is necessary to (i) characterize the properties of composts that control their rates of decomposition and release of n and (ii) determine the optimal amount of composts that should be applied annually to wheat (triticum aestivum l.). carbon and n mineralization were measured under controlled condi ... | 2005 | 15758119 |
| expression of fission yeast cdc25 driven by the wheat adp-glucose pyrophosphorylase large subunit promoter reduces pollen viability and prevents transmission of the transgene in wheat. | cell number was to be measured in wheat (triticum aestivum) endosperm expressing spcdc25 (a fission yeast cell-cycle regulator) controlled by a supposedly endosperm-specific promoter, agp2 (from the large subunit of adp glucose pyrophosphorylase). wheat was transformed by biolistics either with agp2::gus or agp2::spcdc25. pcr and rt-pcr checked integration and expression of the transgene, respectively. in cv. chinese spring, agp2::gus was unexpectedly expressed in carpels and pollen, as well as ... | 2005 | 15760362 |
| evidence for the plasma membrane localization of al-activated malate transporter (almt1). | aluminum (al)-activated malate transporter (almt1) was recently identified from wheat (triticum aestivum). heterologous expression of almt1 led to higher malate exudation that is associated with enhanced al tolerance in transgenic plants. here, we show the first direct evidence that almt1 is localized in the plasma membrane of al-tolerant wheat. phase partitioning experiments showed that this transporter was associated with the plasma membrane fraction. almt1 was detected in an al-tolerant wheat ... | 2005 | 15769806 |
| antioxidant activity of commercial soft and hard wheat (triticum aestivum l.) as affected by gastric ph conditions. | phenolic compounds from soft and hard wheat and their milling fractions were extracted into distilled deionized water, and their in vitro antioxidant activities were evaluated. wheat samples were used as such (nontreated) or subjected to ph adjustment (treated) in order to simulate gastrointestinal ph conditions. the total phenolic content (tpc) was determined using folin-ciocalteu's procedure. the total antioxidant activity (taa) was determined using trolox equivalent antioxidant capacity assay ... | 2005 | 15796575 |
| developmental changes in the metabolic protein profiles of wheat endosperm. | a combined two-dimensional gel electrophoresis-mass spectrometry approach was utilized to identify over 250 proteins of wheat (triticum aestivum l., cv. butte 86) starchy endosperm that participate in 13 biochemical processes: atp interconversion reactions, carbohydrate metabolism, cell division, cytoskeleton, lipid metabolism, nitrogen metabolism, protein synthesis/assembly, protein turnover, signal transduction, protein storage, stress/defense, transcription/translation, and transport. endospe ... | 2005 | 15800972 |
| identification and molecular tagging of a gene from pi 289824 conferring resistance to leaf rust (puccinia triticina) in wheat. | host-plant resistance is the most economically viable and environmentally responsible method of control for puccinia triticina, the causal agent of leaf rust in wheat (triticum aestivum l.). the identification and utilization of new resistance sources is critical to the continued development of improved cultivars as shifts in pathogen races cause the effectiveness of widely deployed genes to be short lived. the objectives of this research were to identify and tag new leaf rust resistance genes. ... | 2005 | 15815925 |
| soft wheat instead of hard wheat in pelleted diets results in high starch digestibility in broiler chickens. | (1) the aim of the experiment was to re-examine variations in digestibilities of food components in 3-week-old broiler chickens fed on pelleted diets based on wheats differing in lipase activity and hardness. fourteen wheat (triticum aestivum) samples, each from a different cultivar, were included at 550 g/kg in 14 different diets given to male ross broiler chicks from 7 d of age. the other main ingredients consisted of soyabean meal (353 g/kg) and rapeseed oil (55 g/kg). a 15th diet containing ... | 2005 | 15835254 |
| allelic reduction and genetic shift in the canadian hard red spring wheat germplasm released from 1845 to 2004. | analysis of genetic diversity changes in existing gene pools of cultivated crops is important for understanding the impact of plant breeding on crop genetic diversity and developing effective indicators for genetic diversity of cultivated plants. the objective of this study was to assess genetic diversity changes in 75 canadian hard red wheat (triticum aestivum l.) cultivars released from 1845 to 2004 using 31 simple sequence repeats (ssrs) markers. a total of 267 ssr alleles were detected, and ... | 2005 | 15841359 |
| genetic analysis of durable resistance to yellow rust in bread wheat. | yellow rust, caused by puccinia striiformis, is one of the most damaging diseases affecting bread wheat in temperate regions. although resistance to yellow rust is frequently overcome by new virulent races, a durable form of resistance in the french bread wheat camp remy (cr) has remained effective since its introduction in 1980. we used 217 f7 recombinant inbred lines (rils) derived from the cross between cr and the susceptible cultivar recital to identify and map quantitative trait loci (qtls) ... | 2005 | 15841362 |
| retention and phytoavailability of radioniobium in soils. | radioniobium is present in long-lived nuclear waste as a result of the activation of zirconium pellets associated with the nuclear fuel. the behaviour of niobium (nb) in the environment and especially its fate in the soil-plant system has not been thoroughly investigated so far. in safety assessment of french long-lived nuclear waste disposal, data concerning the mobility and the bioavailability of nb in soils are needed as well as general trends of its fate in the specific environment around th ... | 2005 | 15511567 |
| scanning electron microscopy of fusarium damaged kernels of spring wheat. | kernels of five wheat cultivars (triticum aestivum) of different bread-making quality were examined. grown under field conditions, heads of wheat were inoculated in the flowering stage with an aqueous suspension of fusarium culmorum conidia. wheat heads were collected from the control and inoculated plots at full maturity. control (non-inoculated) kernels without any symptoms of disease and fusarium damaged kernels (fdk) were examined under scanning electron microscopy (sem). examination of the ... | 2005 | 15681039 |
| long oligonucleotide microarrays in wheat: evaluation of hybridization signal amplification and an oligonucleotide-design computer script. | a computer script was written in the perl language to design equal-length long oligonucleotides from dna sequences. the script allows the user to specify g + c content, melting temperature, self-complementarity, the maximum number of contiguous duplicate bases, whether to start with the first start codon and whether to report reverse complements. microarrays were fabricated with 95 oligonucleotides (60 mers) representing 41 genes. the microarray was interrogated with cdna from roots and shoots o ... | 2005 | 15682265 |
| drought controls on h2o2 accumulation, catalase (cat) activity and cat gene expression in wheat. | plants co-ordinate information derived from many diverse external and internal signals to ensure appropriate control of gene expression under optimal and stress conditions. in this work, the relationships between catalase (cat) and h2o2 during drought in wheat (triticum aestivum l.) are studied. drought-induced h2o2 accumulation correlated with decreases in soil water content and co2 assimilation. leaf h2o2 content increased even though total cat activity doubled under severe drought conditions. ... | 2005 | 15569704 |
| a comparative risk assessment of genetically engineered, mutagenic, and conventional wheat production systems. | wheat (triticum aestivum l.) varieties produced using modern biotechnologies, such as genetic engineering and mutagenic techniques, have lagged behind other crop species, but are now being developed and, in the case of mutagenic wheat, commercially grown around the world. because these wheat varieties have emerged recently, there is a unique opportunity to assess comparatively the potential environmental risks (human health, ecological, and livestock risks) associated with genetically engineered ... | 2005 | 16315092 |
| impact of land use practices on faunal abundance, nutrient dynamics and biochemical properties of desert pedoecosystem. | increased dependence of resource-poor rural communities on soils of low inherent fertility are the major problem of desert agroecosystem. agrisilviculture practices may help to conserve the soil biota for maintaining essential soil properties and processes in harsh climate. therefore, the impacts of different land use systems on faunal density, nutrient dynamics and biochemical properties of soil were studied in agrisilviculture system of indian desert. the selected fields had trees (zizyphus ma ... | 2005 | 16335596 |
| aggregation and mortality of agriotes obscurus (coleoptera: elateridae) at insecticide-treated trap crops of wheat. | agriotes obscurus (l.) wireworms assembled in increasing numbers at rows of treated (agrox dl plus seed treatment) and untreated wheat, triticum aestivum l., planted at increasing densities (0, 0.15, 0.30, and 0.60 seeds/cm). in treated wheat plots at all planting densities, no wireworm damage to seedlings was apparent, and total wireworms taken in core samples in wheat rows increased according to the asymptotic equation y = b0(1 -e(-blx)), where b0 is the asymptote, b1 is the slope of the initi ... | 2005 | 16539125 |
| influence of aphid species and barley yellow dwarf virus on soft red winter wheat yield. | yield loss in soft red winter wheat, triticum aestivum l., caused by aphid-transmitted barley yellow dwarf virus (family luteoviridae, genus luteovirus, bydv) was measured over a 2-yr period in central missouri. rhopalosiphum padi (l.) was the most common and economically important species, accounting for > 90% of the total aphids. schizaphis graminum (rondani), rhopalosiphum maidis (fitch), and sitobion avenae (f.) made up the remainder of the aphids. aphid numbers peaked at wheat stem elongati ... | 2005 | 16539127 |
| insect infestations, incidence of viral plant diseases, and yield of winter wheat in relation to planting date in the northern great plains. | planting date effects on arthropod infestation and viral plant disease are undocumented for winter wheat, triticum aestivum l., in south dakota and the northern great plains. winter wheat was planted over three dates (early, middle, and late; generally from late august to late september) to determine the effect on abundance of insect pests, incidence of plant damage, incidence of viral plant disease, and grain yield. the study was conducted simultaneously at two sites in south dakota over three ... | 2005 | 16539128 |
| effect of plant a-amylase inhibitors on sunn pest, eurygaster integriceps puton (hemiptera: scutelleridae), alpha-amylase activity. | plant-insect interaction is a dynamic system, subjected to continual variation and change. in order to reduce insect attack, plants developed different defence mechanisms including chemical and physical barriers such as the induction of defensive proteins, volatiles that attract predators of the insect herbivores and secondary metabolites. proteinaceous inhibitors of alpha-amylase and proteases are widely distributed in cereals, legumes and some other plants. because of the possible importance o ... | 2005 | 16628930 |
| nematicides increase grain yields in spring wheat cultivars and suppress plant-parasitic and bacterial-feeding nematodes. | grain yields of spring wheat (triticum aestivum l. cvs. ac barrie, ac walton, ac wilmot, belvedere, glenlea) in field plots over a 3-year period were increased (p < 0.001) by an average of 0.56 (25.1%) and 1.17 (52.5%) tonnes/ha in comparison to untreated check plots when aldicarb at 2.24 kg or fosthiazate at 13.5 a.i./ha, respectively, were broadcast and incorporated into the soil to suppress nematodes. the planned f test using orthogonal coefficients indicated that the mean response of grain y ... | 2005 | 19262893 |
| forensic pathology of canadian bread wheat: the case of tan spot. | abstract pyrenophora tritici-repentis causes necrosis and chlorosis in its wheat host. susceptibility to races 2 (necrosis) and 5 (chlorosis) of the pathogen is known to be mediated by ptr toxa and ptr toxb, respectively. sensitivity to each toxin is controlled by a single dominant and independently inherited gene. we used sensitivity to ptr toxa and ptr toxb as two genetic markers to investigate the origin and the state of tan spot susceptibility in canadian western red spring (cwrs) wheat over ... | 2005 | 18943983 |
| resistance of wheat line kavkaz-k4500 l.6.a.4 to septoria tritici blotch controlled by isolate-specific resistance genes. | abstract the international maize and wheat improvement center (cimmyt), mexico, germplasm-derived wheat (triticum aestivum) kavkaz-k4500 l.6.a.4 (kk) is one of the major sources of resistance to septoria tritici blotch (stb). kk is resistant to stb in field conditions in the uk even though a large majority of mycosphaerella graminicola isolates are virulent to it. the genetics of the resistance of kk to four isolates of m. graminicola were investigated. kk has at least five isolate-specific resi ... | 2005 | 18943783 |
| molecular basis of evolutionary events that shaped the hardness locus in diploid and polyploid wheat species (triticum and aegilops). | the hardness (ha) locus controls grain hardness in hexaploid wheat (triticum aestivum) and its relatives (triticum and aegilops species) and represents a classical example of a trait whose variation arose from gene loss after polyploidization. in this study, we investigated the molecular basis of the evolutionary events observed at this locus by comparing corresponding sequences of diploid, tertraploid, and hexaploid wheat species (triticum and aegilops). genomic rearrangements, such as transpos ... | 2005 | 15749759 |
| complementation of sugary-1 phenotype in rice endosperm with the wheat isoamylase1 gene supports a direct role for isoamylase1 in amylopectin biosynthesis. | to examine the role of isoamylase1 (isa1) in amylopectin biosynthesis in plants, a genomic dna fragment from aegilops tauschii was introduced into the isa1-deficient rice (oryza sativa) sugary-1 mutant line em914, in which endosperm starch is completely replaced by phytoglycogen. a. tauschii is the d genome donor of wheat (triticum aestivum), and the introduced fragment effectively included the gene for isa1 for wheat (taisa1) that was encoded on the d genome. in taisa1-expressing rice endosperm ... | 2005 | 15618430 |
| association mapping of kernel size and milling quality in wheat (triticum aestivum l.) cultivars. | association mapping is a method for detection of gene effects based on linkage disequilibrium (ld) that complements qtl analysis in the development of tools for molecular plant breeding. in this study, association mapping was performed on a selected sample of 95 cultivars of soft winter wheat. population structure was estimated on the basis of 36 unlinked simple-sequence repeat (ssr) markers. the extent of ld was estimated on chromosomes 2d and part of 5a, relative to the ld observed among unlin ... | 2005 | 16079235 |
| hfr-2, a wheat cytolytic toxin-like gene, is up-regulated by virulent hessian fly larval feedingdouble dagger. | summary both yield and grain-quality are dramatically decreased when susceptible wheat (triticum aestivum) plants are infested by hessian fly (mayetiola destructor) larvae. examination of the changes in wheat gene expression during infestation by virulent hessian fly larvae has identified the up-regulation of a gene, hessian fly responsive-2 (hfr-2), which contains regions similar to genes encoding seed-specific agglutinin proteins from amaranthus. hfr-2, however, did not accumulate in developin ... | 2005 | 20565667 |
| metabolic response to treatment with cold, paraquat, or 3-amino-1,2,4-triazole in leaves of winter wheat. | we treated leaves of winter wheat (triticum aestivum l.) with cold, paraquat, or 3-amino-1,2,4-triazole and compared the responses. we assayed the activities of glucose-6-phosphate dehydrogenase, catalase, dehydroascorbate reductase and ascorbate free radical reductase and levels of hydrogen peroxide, glucose-6-phosphate, fructose-6-phosphate, ascorbate, dehydroascorbate, reduced and oxidized glutathione. with any of the three treatments, contents of cellular peroxides and hexose phosphates were ... | 2005 | 1369090 |
| endocytosis and vesicle trafficking during tip growth of root hairs. | the directional elongation of root hairs, "tip growth", depends on the coordinated and highly regulated trafficking of vesicles which fill the tip cytoplasm and are active in secretion of cell wall material. so far, little is known about the dynamics of endocytosis in living root hairs. we analyzed the motile behaviour of vesicles in the apical region of living root hairs of arabidopsis thaliana and of triticum aestivum by live cell microscopy. for direct observation of endocytosis and of the fa ... | 2005 | 16231100 |
| plant-based microarray data at the european bioinformatics institute. introducing atmiamexpress, a submission tool for arabidopsis gene expression data to arrayexpress. | arrayexpress is a public microarray repository founded on the minimum information about a microarray experiment (miame) principles that stores miame-compliant gene expression data. plant-based data sets represent approximately one-quarter of the experiments in arrayexpress. the majority are based on arabidopsis (arabidopsis thaliana); however, there are other data sets based on triticum aestivum, hordeum vulgare, and populus subsp. atmiamexpress is an open-source web-based software application f ... | 2005 | 16219923 |
| wheat cultivar-specific proteins in grain revealed by 2-de and their application to cultivar identification of flour. | wheat flour proteins were studied to identify the cultivar-specific proteins and use them to identify cultivars in flours. proteins extracted from flours of japanese wheat (cultivars hokushin, horoshirikomugi, kitanokaori and kachikei 33) and canadian wheat (canada western red spring wheat no. 1; 1cw) were analyzed by 2-de with ief gels over three ph ranges: ph 4-7, ph 5-8, and ph 6-11. this system enabled detection of more than 1600 protein spots. we recognized that among 50 protein spots showi ... | 2005 | 16152659 |
| regulation of vrn-1 vernalization genes in normal and transgenic polyploid wheat. | vernalization, the requirement of a long exposure to low temperatures to accelerate flowering, is an essential adaptation of plants to cold winters. the vernalization gene vrn-1 plays an important role in this process in diploid (triticum monococcum) and polyploid wheat (triticum aestivum). we have recently shown that the diploid wheat vrn-a(m)1 gene was similar to the arabidopsis (arabidopsis thaliana l. heynh.) apetala1 meristem identity gene. we also showed that dominant vrn-a(m)1 alleles wer ... | 2005 | 16055679 |
| systematic spatial analysis of gene expression during wheat caryopsis development. | the cereal caryopsis is a complex tissue in which maternal and endosperm tissues follow distinct but coordinated developmental programs. because of the hexaploid genome in wheat (triticum aestivum), the identification of genes involved in key developmental processes by genetic approaches has been difficult. to bypass this limitation, we surveyed 888 genes that are expressed during caryopsis development using a novel high-throughput mrna in situ hybridization method. this survey revealed novel di ... | 2005 | 16006577 |
| comparative studies on codon usage pattern of chloroplasts and their host nuclear genes in four plant species. | a detailed comparison was made of codon usage of chloroplast genes with their host (nuclear) genes in the four angiosperm species oryza sativa, zea mays, triticum aestivum and arabidopsis thaliana. the average gc content of the entire genes, and at the three codon positions individually, was higher in nuclear than in chloroplast genes, suggesting different genomic organization and mutation pressures in nuclear and chloroplast genes. the results of nc-plots and neutrality plots suggested that nuc ... | 2005 | 15876584 |
| structure-activity relationships (sar) studies of benzoxazinones, their degradation products and analogues. phytotoxicity on standard target species (sts). | benzoxazinones 2,4-dihydroxy-7-methoxy-(2h)-1,4-benzoxazin-3(4h)-one (dimboa) and 2,4-dihydroxy-(2h)-1,4-benzoxazin-3(4h)-one (diboa) have been considered key compounds for understanding allelopathic phenomena in gramineae crop plants such as corn (zea mays l.), wheat (triticum aestivum l.), and rye (secale cereale l.). the degradation processes in the environment observed for these compounds, in which soil microbes are directly involved, could affect potential allelopathic activity of these pla ... | 2005 | 15686399 |
| degradation studies on benzoxazinoids. soil degradation dynamics of (2r)-2-o-beta-d-glucopyranosyl-4-hydroxy-(2h)- 1,4-benzoxazin-3(4h)-one (diboa-glc) and its degradation products, phytotoxic allelochemicals from gramineae. | wheat (triticum aestivum l.) has been found to possess allelopathic potential and studies have been conduced to apply wheat allelopathy for biological weed control. 2,4-dihydroxy-(2h)-1,4-benzoxazin-3(4h)-one (diboa) is a common product found in wheat, corn, and rye exudates and it can be released to the environment by that way. in this report, the stability of diboa is studied in two soils from crop lands of wheat cv. astron and cv. ritmo. these varieties were selected by their concentrations o ... | 2005 | 15686401 |
| uptake of sodium in quince, sugar beet, and wheat protoplasts determined by the fluorescent sodium-binding dye benzofuran isophthalate. | the uptake of sodium into protoplasts of quince (cydonia oblonga mill, clone ba29), sugar beet (beta vulgaris l. cv. monohill), and wheat (triticum aestivum l. cv. kadett) was determined by use of the acetoxy methyl ester of the fluorescent sodium-binding benzofuran isopthalate (sbfi-am). in the presence of 1 mm cacl2, little sodium was taken up in the cytosol of quince mesophyll cells compared to cytosols of sugar beet and wheat. upon addition of 40 mm nacl, approximately the same amount of sod ... | 2005 | 15900884 |
| imidazolinone-tolerant crops: history, current status and future. | imidazolinone herbicides, which include imazapyr, imazapic, imazethapyr, imazamox, imazamethabenz and imazaquin, control weeds by inhibiting the enzyme acetohydroxyacid synthase (ahas), also called acetolactate synthase (als). ahas is a critical enzyme for the biosynthesis of branched-chain amino acids in plants. several variant ahas genes conferring imidazolinone tolerance were discovered in plants through mutagenesis and selection, and were used to create imidazolinone-tolerant maize (zea mays ... | 2005 | 15627242 |
| graingenes 2.0. an improved resource for the small-grains community. | graingenes (http://wheat.pw.usda.gov) is an international database for genetic and genomic information about triticeae species (wheat [triticum aestivum], barley [hordeum vulgare], rye [secale cereale], and their wild relatives) and oat (avena sativa) and its wild relatives. a major strength of the graingenes project is the interaction of the curators with database users in the research community, placing graingenes as both a data repository and information hub. the primary intensively curated d ... | 2005 | 16219925 |
| competitive interactions among four pest species of earth mites (acari: penthaleidae). | earth mites are major winter pests of a variety of crops and pastures in southern australia. competition between four earth mite species was investigated using field and shadehouse experiments. the influence of different plant hosts on the frequency and intensity of competitive interactions also were examined. this information is important, because control attempts that eradicate one species of mite could be directly followed by an increase in abundance of another earth mite species. there were ... | 2005 | 15889718 |
| [effects of cultured astragalus adsurgens root on other plants growth]. | by using orthogonal design, this paper investigated the growth of cultured prairie milk vetch (astragalus adsurgens) root on the b5 medium with different contents of sucrose, macro-and microelements and organic supplements, and adopting the method of filter paper-culture in petri-dish, studied the effects of a. adsurgens culture filtrate on the seedling growth of radish (raphanus sativus) and wheat (triticum aestivum). the results showed that the effect of a. adsurgens culture filtrate on r. sat ... | 2005 | 16011183 |
| plant-parasitic nematodes associated with reduced wheat yield in oregon: heterodera avenae. | heterodera avenae is widely distributed in the western united states, where most wheat is grown in non-irrigated winter wheat/summer fallow rotations in low rainfall regions. economic and social pressures have motivated growers to pursue a transition from winter wheat/summer fallow rotation to no-till annual spring cereals. annual cereals are also planted in some irrigated fields. the impact of h. avenae on spring wheat yield in the pacific northwest had been observed but not quantified. spring ... | 2005 | 19262877 |
| impact of cre1, cre8 and cre3 genes on cereal cyst nematode resistance in wheat. | the cereal cyst nematode (ccn; heterodera avenae), a root disease of cereal crops, is a major economic constraint in many wheat (triticum aestivum)-growing areas of the world. the objective of this study was to assess the impact of the cre1, cre8 and cre3 genes on ccn resistance. a population of 92 doubled-haploid (dh) lines derived from a cross between wheat cvs. frame and silverstar as well as 1,851 wheat breeding lines were screened for ccn resistance at the primary industries research victor ... | 2005 | 15655664 |
| pratylenchus thornei associated with reduced wheat yield in oregon. | pratylenchus thornei reaches high population densities in non-irrigated annual cropping systems in low-rainfall regions of the pacific northwest. two spring wheat varieties with different levels of tolerance and susceptibility to p. thornei were treated or not treated with aldicarb in three experiments. grain yield was inversely correlated (p < 0.05) with pre-plant populations of p. thornei in soil and with p. thornei density in mature roots. as population of p. thornei increased, yield of the m ... | 2005 | 19262842 |
| resistance to rhopalosiphum padi (homoptera: aphididae) in three triticale accessions. | experiments were conducted to identify and characterize host plant resistance to bird cherry-oat aphid, rhopalosiphum padi (l.), in various wheat and wheat-grass hybrids. initial tests screened for resistance to r. padi among 12 grass accessions (eight wheat [triticum aestivum l.], three triticale [xtriticosecale wittmack], and 1xelytricum [elytrigia elongata [host] nevski x triticum aestivum hybrid]). r. padi had less population growth on triticale accessions '8ta5l' (pi 611760) and 'stniism 3' ... | 2005 | 15889754 |
| biofumigant compounds released by field pennycress (thlaspi arvense) seedmeal. | defatted field pennycress (thlaspi arvense l.) seedmeal was found to completely inhibit seedling germination/emergence when added to a sandy loam soil containing wheat (triticum aestivum l.) and arugula [eruca vesicaria (l.) cav. subsp. sativa (mill.) thell.] seeds at levels of 1.0% w/w or higher. covering the pots with petri dishes containing the soil-seedmeal mixture decreased germination of both species at the lowest application rate (0.5% w/w), suggesting that the some of the phytotoxins wer ... | 2005 | 15839488 |
| interactions among three species of cereal aphids simultaneously infesting wheat. | interactions among greenbug, schizaphis graminum (rondani), russian wheat aphid, diuraphis noxia (mordvilko), and bird cherry-oat aphid rhopalosiphum padi (l.) were examined on wheat plants (triticum aestivum l., cultivar tam 107). nymphs were released on the plants as conspecific and heterospecific pairs of either first or fourth instars and evaluated for survival, developmental time, fecundity, intra-plant movement, and affinity to plant tissues. survival from first instar to onset of reproduc ... | 2005 | 16341245 |
| comparison of dn4- and dn7-carrying spring wheat genotypes artificially infested with russian wheat aphid (homoptera: aphididae) biotype 1. | genetic resistance is a useful control strategy for managing russian wheat aphid, diuraphis noxia (mordvilko), in wheat, triticum aestivum l. in 2003, a russian wheat aphid population (denoted as biotype 2) identified in colorado was virulent to genotypes carrying the dn4 russian wheat aphid resistance gene, necessitating the rapid identification and deployment of new sources of resistance. although the dn7 gene had shown excellent resistance to russian wheat aphid biotypes 1 and 2 in evaluation ... | 2005 | 16334342 |
| effect of four cropping systems on variant western corn rootworm (coleoptera: chrysomelidae) adult and egg densities and subsequent larval injury in rotated maize. | the cultural practice of rotating corn, zea mays l., with soybean, glycine max (l.) merrill, to manage larval injury by the western corn rootworm, diabrotica virgifera virgifera leconte, was used extensively throughout east central illinois and northern indiana until the mid-1990s. the effectiveness of this management tactic diminished due to a shift in the ovipositional behavior of the western corn rootworm. the variant western corn rootworm has since spread as far as northwestern illinois, sou ... | 2005 | 16334328 |
| aerobic stability of wheat and orchardgrass round-bale silages during winter. | using recently developed technology, balage is often stored in large (1.2 x 1.2 m) round bales that are wrapped in plastic film with an in-line wrapper. the aerobic stability of this fermented forage is important, particularly during winter months when it is fed to livestock or sold as a cash crop. two types of forage, orchardgrass [dactylis glomerata l.; 54.4% dry matter (dm)] and wheat (triticum aestivum l.; 62.4% dm), were packaged in large round bales and wrapped with an in-line wrapper duri ... | 2005 | 15829675 |
| [allelopathic effects of cultured cucurbita moschata root exudates]. | by using the techniques of tissue culture, bio-assay and laboratory analysis, this paper studied the effects of the allelopathic chemicals from pumpkin (cucurbita moschata) roots on the seed germination and seedling growth of pumpkin, wheat (triticum aestivum), and radish (raphanus sativus). the pumpkin root was cultured on a sterile b5 media, and the concentrations of macro- and microelements, organic supplements and hormones in the media were adjusted by using an orthogonal design. after cultu ... | 2005 | 16011180 |
| diversification in substrate usage by glutathione synthetases from soya bean (glycine max), wheat (triticum aestivum) and maize (zea mays). | unlike animals which accumulate glutathione (gamma-glutamyl-l-cysteinyl-glycine) alone as their major thiol antioxidant, several crops synthesize alternative forms of glutathione by varying the carboxy residue. the molecular basis of this variation is not well understood, but the substrate specificity of the respective gss (glutathione synthetases) has been implicated. to investigate their substrate tolerance, five gs-like cdnas have been cloned from plants that can accumulate alternative forms ... | 2005 | 16008521 |
| cereal phosphate transporters associated with the mycorrhizal pathway of phosphate uptake into roots. | a very large number of plant species are capable of forming symbiotic associations with arbuscular mycorrhizal (am) fungi. the roots of these plants are potentially capable of absorbing p from the soil solution both directly through root epidermis and root hairs, and via the am fungal pathway that delivers p to the root cortex. a large number of phosphate (p) transporters have been identified in plants; tissue expression patterns and kinetic information supports the roles of some of these in the ... | 2005 | 16133217 |
| chemical alteration of the rhizosphere of the mycorrhizal-colonized wheat root. | plexiglass pot growth chamber experiments were conducted to evaluate the chemical alterations in the rhizosphere of mycorrhizal wheat roots after inoculation with glomus intraradices [arbuscular mycorrhizal fungus (amf)]. exchange resins were used as sinks for nutrients to determine whether the inoculated plant can increase the solubility and the uptake of p and micronutrients. treatments included: (1) soil (bulk soil); (2) amf inoculation no p addition (i-p); (3) no inoculation with no p additi ... | 2005 | 15503187 |
| microsatellite dna polymorphism divergence in chinese wheat (triticum aestivum l.) landraces highly resistant to fusarium head blight. | genetic differences between 20 chinese wheat (triticum aestivum l.) landraces highly resistant to fusarium head blight (fhb) and 4 wheat lines highly susceptible to fhb were evaluated by means of microsatellite markers, in order to select suitable parents for gene mapping studies. thirty-nine out of 40 microsatellite markers (97.5%) were polymorphic among the 24 wheat genotypes. a total of 276 alleles were detected at the 40 microsatellite loci. the number of alleles per locus ranged from 1 to 1 ... | 2005 | 15741658 |
| [ecological toxicity of aluminum-based coagulant on representative corps in neutral environment]. | by the terrestrial ecotoxicological methods of seed germination and root elongation, and with aluminum-based coagulant alcl3 which was widely used in wastewater- and water treatment as an example, this work studied the toxicological effects of aluminum-based coagulants on representative crops, including radish raphanus sativus, cabbage brassica chinensis and wheat triticum aestivum, over a range of exposure concentrations under the neutral condition (ph= 7.0), with contrast to an acid condition ... | 2005 | 16471362 |
| real-time polymerase chain reaction based assays for quantitative detection of barley, rice, sunflower, and wheat. | quality assurance is a major issue in the food industry. the authenticity of food ingredients and their traceability are required by consumers and authorities. plant species such as barley (hordeum vulgare), rice (oryza sativa), sunflower (helianthus annuus), and wheat (triticum aestivum) are very common among the ingredients of many processed food products; therefore the development of specific assays for their specific detection and quantification are needed. furthermore, the production and tr ... | 2005 | 16131102 |
| physiological and genetic analyses of aluminium tolerance in rice, focusing on root growth during germination. | aluminium (al) ion limits root growth of plants in acidic soils, and rice exhibits the highest level of al-tolerance among graminous crops. to elucidate al-tolerance mechanisms in rice, response to al was compared between rice (oryza sativa l., cv. nipponbare) and wheat (triticum aestivum l., cv. et8), focusing on seminal root growth at seedling stage and germination stage. at seedling stage, rice and wheat were similarly sensitive to al in both dose- and time-dependent manner during a 24-h al e ... | 2005 | 16095709 |
| large intraspecific haplotype variability at the rph7 locus results from rapid and recent divergence in the barley genome. | to study genome evolution and diversity in barley (hordeum vulgare), we have sequenced and compared more than 300 kb of sequence spanning the rph7 leaf rust disease resistance gene in two barley cultivars. colinearity was restricted to five genic and two intergenic regions representing <35% of the two sequences. in each interval separating the seven conserved regions, the number and type of repetitive elements were completely different between the two homologous sequences, and a single gene was ... | 2005 | 15659632 |
| [effects of various steroids of corticotropin (acth) on the processes of germination & growth of seeds of triticum vulgare & phaseolus vulgaris]. | 2005 | 13591741 | |
| intracellular fitc-derivatization with peg. | in order to investigate the amino acids (aas) in plant cells, we explore an avenue for intracellular derivatization with fitc. in this method, fitc was used to mark aas in living protoplasts derived from embryogenic calli of common wheat (triticum aestivum l. c.v. jinan 177) mediated by peg. after fitc-derivatization, the aas in the lysate were determined by ce. the result reveals that this peg method can be used to transfer fitc into plant cells efficiently, which provides a good method for aa ... | 2005 | 16217827 |
| nsltp1 and nsltp2 isoforms in soft wheat (triticum aestivum cv. centauro) and farro (triticum dicoccon schrank) bran. | isoforms of nonspecific lipid-transfer protein 1 (nsltp1) and nonspecific lipid-transfer protein 2 (nsltp2) were investigated in bran tissues isolated from caryopses of two cereal crops quite relevant for the italian market, the cultivar centauro of soft wheat (triticum aestivum) and italian emmer or farro (triticum dicoccon schrank). by sequential separation of the bran extracts on cation-exchange and gel filtration chromatographies, fractions containing only proteins belonging to the nsltp1 an ... | 2005 | 16190659 |
| n-acylanilines, herbicide-cha chimera, and amino acid analogues as novel chemical hybridizing agents for wheat (triticum aestivum l.). | in the absence of viable alternative technology of hybrid wheat development, chemical induction of male sterility mediated technology based on chemical hybridizing agents (chas) holds a great potential. n-acylaniline derivatives, namely, ethyl 4'-fluoro oxanilate (1) and ethyl 4'-trifluoromethyl oxanilate (2) containing halogen atoms in the para position of the aryl ring and substituted amide linkage (-co-nh-) in the acyl side chain induced >98% spikelet sterility on three genotypes of wheat, na ... | 2005 | 16190648 |
| molecular mapping of a quantitative trait locus for aluminum tolerance in wheat cultivar atlas 66. | genetic improvement of aluminum (al) tolerance is one of the cost-effective solutions to improve wheat (triticum aestivum) productivity in acidic soils. the objectives of the present study were to identify quantitative trait loci (qtl) for al-tolerance and associated pcr-based markers for marker-assisted breeding utilizing cultivar atlas 66. a population of recombinant inbred lines (rils) from the cross atlas 66/century was screened for al-tolerance by measuring root-growth rate during al treatm ... | 2005 | 16189660 |
| production of fertile transgenic wheat plants by laser micropuncture. | a modified, non-damaging, protocol for the production of fertile transgenic wheat (triticum aestivum l. cultivar giza 164) plants by laser micropuncture was developed. the new homemade setup secures the transformation of as many as 60 immature embryo-derived calli (10000 cells each) in less than one hour using a uv excimer laser with two dimensional translation stages, a suitable computer program and a proper optical system. five-day-old calli were irradiated by a focused laser microbeam to punc ... | 2005 | 16189555 |
| isolation and characterization of tadof1 transcription factor in wheat (triticum. aestivum. l). | the dof (dna binding with one finger) proteins are plant specific transcription factors. dof proteins are apparently encoded by a multiple gene family in higher plants. however, only one dof gene, wpbf, was reported in wheat. in this study, a member of dof gene family, tadof1, was cloned from wheat. tadof1 encode 291 amino acids, with a predicted molecular mass of 30.348 kda. at its n-terminal end, a 52 amino acid stretch typical of dof domain and two serine-rich stretches were observed. sequenc ... | 2005 | 16243726 |
| [molecular analysis of the triticale lines with different vrn gene systems using microsatellite markers and hybridization in situ]. | hexaploid triticale (x triticosecale wittmack) lines were examined using molecular markers and the hybridization in situ technique. triticale lines were generated based on wheat varieties differing by the vrn gene systems and the earing times. molecular analysis was performed using xgwm and xrms microsatellite markers with the known chromosomal localization in the common wheat triticum aestivum, and rye secale cereale genomes. comparative molecular analysis of triticale lines and their parental ... | 2005 | 16240635 |
| two plant puroindolines colocalize in wheat seed and in vitro synergistically fight against pathogens. | puroindolines, for years largely investigated for their involvement in wheat kernel hardness, have recently attracted attention thanks to their possible role as antimicrobial proteins. with the aim to enhance our knowledge of these proteins we studied their localization in the kernel, and their antimicrobial activity in vitro against six different bacterial strains. immunolocalization showed that both the pins are strongly concentrated in the aleurone layer, but also highly present in the endosp ... | 2005 | 16240178 |
| dgge fragments oscillate with or counter to fluctuations in cultivable bacteria along wheat roots. | previously, we showed that bacterial populations oscillate in response to a moving substrate source such as a root tip, resulting in moving wavelike distributions along roots. for this article, we investigated if bacterial communities fluctuate as a whole or if there is a succession in bacterial composition from peak to peak or within peaks. rhizosphere microbial communities along roots of wheat triticum aestivum l. were studied in detail (20-25 rhizosphere and bulk soil samples along the total ... | 2005 | 16307384 |
| expression profile of two storage-protein gene families in hexaploid wheat revealed by large-scale analysis of expressed sequence tags. | to discern expression patterns of individual storage-protein genes in hexaploid wheat (triticum aestivum cv chinese spring), we analyzed comprehensive expressed sequence tags (ests) of common wheat using a bioinformatics technique. the gene families for alpha/beta-gliadins and low molecular-weight glutenin subunit were selected from the est database. the alignment of these genes enabled us to trace the single nucleotide polymorphism sites among both genes. the combinations of single nucleotide p ... | 2005 | 16306141 |
| [relationship of microtubule and [ca2+]cyt in wheat mesophyll protoplast]. | by using immunofluorescence labeling technique and incubating the fluo-3am of ca2+ fluorescein, wheat (triticum aestivum) cultivars lovrin 10 was selected as mesophyll protoplast material in this paper for studying the relationship between the pattern of microtubule and [ca2+]cyt. laser scanning confocal microscopy(lscm) was used and pharmacologic examination was set. the results indicated there was a connection between calcium signaling and microtubule organization in wheat mesophyll protoplast ... | 2005 | 16231700 |
| an alternative to radiation hybrid mapping for large-scale genome analysis in barley. | the presence of a monosomic gametocidal chromosome (gc) in a barley chromosome addition line of common wheat generates structural aberrations in the barley chromosome as well as in the wheat chromosomes of gametes lacking the gc. a collection of structurally aberrant barley chromosomes is analogous to a panel of radiation hybrid (rh) mapping and is valuable for high-throughput physical mapping. we developed 90 common wheat lines (gc lines) containing aberrant barley 7h chromosomes induced by a g ... | 2005 | 16231150 |
| novel puroindoline and grain softness protein alleles in aegilops species with the c, d, s, m and u genomes. | dna from six hexaploid, tetraploid and diploid species of aegilops with the c, d, s, m and u genomes was amplified with specific pcr primers to identify sequences encoding puroindolines (pins) a and b and grain softness protein (gsp), all of which are encoded by genes at the ha (hardness) locus, with ae. tauschii (dd) and bread wheat (t. aestivum) (aabbdd) cv hiline being studied as controls. seven new allelic forms of pin a and pin b were identified, including forms with mutations within or clo ... | 2005 | 16133313 |
| introgression of wheat dna markers from a, b and d genomes in early generation progeny of aegilops cylindrica host x triticum aestivum l. hybrids. | introgression from allohexaploid wheat (triticum aestivum l., aabbdd) to allotetraploid jointed goatgrass (aegilops cylindrica host, ccdd) can take place in areas where the two species grow in sympatry and hybridize. wheat and ae. cylindrica share the d genome, issued from the common diploid ancestor aegilops tauschii coss. it has been proposed that the a and b genome of bread wheat are secure places to insert transgenes to avoid their introgression into ae. cylindrica because during meiosis in ... | 2005 | 16133306 |
| a 'chinese spring' wheat (triticum aestivum l.) bacterial artificial chromosome library and its use in the isolation of ssr markers for targeted genome regions. | a bacterial artificial chromosome (bac) library was constructed from the bread wheat (triticum aestivum l.) genotype 'chinese spring' ('cs'). the library consists of 395,136 clones with an estimated average insert size of 157 kb. this library provides an estimated 3.4-fold genome coverage for this hexaploid species. the genome coverage was confirmed by rflp analysis of single-copy rflp clones. the cs bac library was used to develop simple sequence repeat (ssr) markers for targeted genome regions ... | 2005 | 16187119 |
| genetic and physiological architecture of early vigor in aegilops tauschii, the d-genome donor of hexaploid wheat. a quantitative trait loci analysis. | plant growth can be studied at different organizational levels, varying from cell, leaf, and shoot to the whole plant. the early growth of seedlings is important for the plant's establishment and its eventual success. wheat (triticum aestivum, genome aabbdd) seedlings exhibit a low early growth rate or early vigor. the germplasm of wheat is limited. wild relatives constitute a source of genetic variation. we explored the physiological and genetic relationships among a range of early vigor traits ... | 2005 | 16183848 |
| assembling complex genotypes to resist fusarium in wheat (triticum aestivum l.). | fusarium head blight of wheat is a major deterrent to wheat production world-wide. the genetics of fhb resistance in wheat are becoming clear and there is a good understanding of the genome location of fhb resistance qtl from different sources such as sumai3, wuhan, nyubai and frontana. all the components needed for assembling complex genotypes through large-scale molecular breeding experiments are now available. this experiment used high throughput microsatellite genotyping and half-seed analys ... | 2005 | 16179996 |