Publications

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loss of dna behind the growing point of thymine-starved bacillus subtilis 168. 19704991007
regulation of competence development in bacillus subtilis. 19704991028
related base sequences in the dna of simple and complex organisms. v. the specificity of interactions between oligonucleotides and denatured dna. 19704991031
related base sequences in the dna of simple and complex organisms. vi. the extent of base sequence divergence among the dnas of various rodents. 19704991032
new ideas and data on competence and dna entry in transformation of bacillus subtilis. 19704991054
[bronchial asthma following occupational exposure to proteolytic enzymes (bacillus subtilis proteases)]. 19704991076
relationship between competence and doubling time in bacillus subtilis cultures. 19704991104
[determination acid and a specific inhibitor]. 19704991157
some aspects of the active transport of amino acids. 19704987442
effect of extraneous particulate matter on airborne bacteria. 19704987500
translational complementarity of rna transcripts of native and denatured b. subtilis dna.previous evidence has suggested that the l and h fractions into which denatured dna of b. subtilis can be separated by chromatography are complementary to each other with regard to base compositon, nucleotide sequence, and template properties, and that they may be regarded as families of fragments of the respective dna strands. the present study tests the proposition that these fractions should also exhibit features of translational complementarity with respect to the dna-dependent or the rna-de ...19704987628
mutations and loss of transforming activity of bacillus subtilis dna after reaction with esters of carcinogenic n-hydroxy aromatic amides. 19704987694
genetic properties of a membrane-bound fraction of dna in the culture of bacillus subtilis with synchronously replicating chromosomes. 19704987736
bacterial flagella: polarity of elongation.newly synthesized regions of bacillus subtilis flagella were labeled with fluorophenylalanine or [(3)h]leucine. the flagella were then examined for altered gross morphology or by radioautography. results of both experiments indicate that flagella elongate in vivo by polymerization of flagellin subunits onto the distal end of the filament.19704987789
respiratory symptoms and washing powders. 19704987802
cell septation during synchronous growth of bacillus subtilis. 19704987876
[antimicrobial contents of algae. 2. presence of acrylic acid in different sea alga]. 19704987880
survey of workers exposed to dusts containing derivatives of bacillus subtilis.in a survey of 121 workers exposed to dusts containing derivatives of bacillus subtilis, mainly proteolytic enzymes, skin tests showed evidence of sentiztation was higher among "atopic" subjects-16 out of tization was higher among "atopic" subjects-16 out of 25 (64%)-than among "non-atopic" subjects-32 out of 96 (33%). reduced ventilatory capacity was found in 44% of sensitized workers compared with 14% of those not sensitized.19704987928
effect of2, 2'-(9,10-anthrylenedimethylene)bis(2-thiopseudourea) on nucleic acid synthesis in bacteria. 19704987957
gene expression after transformation of bacillus subtilis.sensitive assays of histidase activity were used to follow the production of this enzyme as directed by a gene newly introduced into cells of bacillus subtilis by transformation. histidase activity can be detected in histidase-negative recipient cells within 1 hr after the addition of deoxyribonucleic acid extracted from histidase-positive donors. enzyme production continues for one to two additional hours and then ceases. histidase production in the transformed cells is fully sensitive to catab ...19704988038
repair of alkylation damage: stability of methyl groups in bacillus subtilis treated with methyl methanesulfonate.bacillus subtilis was not inactivated and was able to replicate even though approximately 3 x 10(4) methyl groups added by methyl methanesulfonate (mms) were bound to the deoxyribonucleic acid (dna) of each organism. no significant loss of methyl groups from the dna occurred for several generations upon incubation of methylated wild-type or mms-sensitive cells. single-strand breaks were not observed in the dna from cells treated at this low mms dose. higher doses of mms resulted in significant k ...19704988041
intercellular effects on development of competence in bacillus subtilis.the intercellular transfer of competence during growth under the conditions specified by the transformation procedure of spizizen was investigated with bacillus subtilis 168. the rate of competence development as assayed uniformly in medium b was not affected by variations in the cell concentration, although the first appearance of transformants occurred earlier with high cell densities in medium a, approximately in proportion to the onset of the stationary phase in the culture. growth in the pr ...19704988042
effect of miracil d on marker frequency ratio and cytotoxicity in bacillus subtilis.when miracil d was added to mid-log phase bacillus subtilis cells, the rate of growth decreased immediately, and the turbidity of the culture began to decrease within 15 to 20 min after addition of the drug. at this stage, ghostlike cells were observed under phase-contrast microscopy. the viable count also began to decrease rapidly after 15 min in the presence of the drug, and within 60 min there was a 10,000-fold reduction in viability. incorporation of (3)h-thymidine into deoxyribonucleic acid ...19704988044
isolation of l-forms of bacillus subtilis which grow in liquid medium.l-forms of bacillus subtilis can be isolated by treatment of the parent strain sequentially with n-methyl-n'-nitro-n-nitrosoguanidine and lysozyme and selection of the surviving protoplasts on semisolid medium containing 2,000 units of penicillin per ml. some of these clones can be adapted to grow in liquid cultures containing 1.2 m nacl. this method will aid in the isolation of cell wall mutants which require hypertonic medium for growth.19704988045
correlation between pigment production and amino acid requirements in bacillus subtilis.several bacillus subtilis w-23 auxotrophs were unable to produce wild-type pigment normally on minimal agars supplemented sufficiently for growth. this offers a reliable means for scoring genotypes.19704988046
isolation and properties of suppressor-sensitive mutants of bacillus subtilis bacteriophage sp02.suppressor-sensitive mutants of bacillus subtilis phage spo2 were isolated and classified into 18 cistrons. a linear genetic map was constructed. the genes controlling early functions of phage development were clustered near the ci gene which may produce phage repressor.19704988052
symposium on bacterial spores: ii. genetics of sporulation in bacillus subtilis marburg. 19704986700
symposium on bacterial spores: xv. spores in milk: problems associated with uht processing. 19704986701
characterization of proteinases excreted by bacillus subtilis marburg strain during sporulation. 19704986703
physiological studies on early-blocked sporulation mutants of bacillus subtilis. 19704986704
effect of the method of presentation of the medium on the germination and growth of single spores of bacillus subtilis. 19704986705
symposium on bacterial spores: 3. biochemical studies of spore core and coat protein synthesis. 19704986706
pulmonary absorption and retention of kanamycin after repeated inhalation administration of kanamycin aerosol.the absorption and retention of kanamycin by the pulmonary system was evaluated after aerosol administration to rats. tissue level concentrations of the drug were assayed by the agar-plate bioassay method. mean concentrations of 6.58 mug/g and 3.52 mug/g were found in the lung and kidney homogenates, respectively, 26 hr after the final exposure period. no detectable level was found in the plasma.19704986727
formation of protoporphyrin from coproporphyrinogen in extracts of various bacteria.the enzyme system capable of converting coproporphyrinogen to protoporphyrin was demonstrated in the soluble fraction of extracts of pseudomonas fluorescens grown aerobically, of p. denitrificans grown anaerobically under denitrifying conditions, and of escherichia coli grown both aerobically and anaerobically. protoporphyrin accumulation by each of these extracts occurred only if the assay was conducted aerobically. attempts to replace this oxygen requirement with several alternate electron acc ...19704986760
relative affinity of vancomycin and ristocetin for cell walls and uridine diphosphate-n-acetylmuramyl pentapeptide.a determination of the relative affinity of vancomycin and ristocetin for isolated cell walls and for a peptidoglycan precursor was made. these antibiotics had previously been shown to adsorb to cell walls and to complex with peptides containing a d-alanyl-d-alanine c-terminus. by using (14)c-uridine diphosphate (udp)-n-acetylmuramyl pentapeptide, it was shown that the complex which is formed between this peptidoglycan precursor and either vancomycin or ristocetin does not preclude adsorption of ...19704986761
action of mycobacillin on filamentous fungi. 19704986826
bacteriology of fallopian tube in relation to puerperal sterilization. 19704986847
characterization of infectious deoxyribonucleic acid from temperature bacillus subtilis bacteriophage phi105.phenol-extracted, infectious deoxyribonucleic acid (dna) species from phi105 phage particles, from phi105 lysogenic bacteria, and from induced phi105 lysogenic bacteria were sedimented in sucrose gradients. infectious dna from phi105 particles sedimented like the bulk of mature phage dna in neutral sucrose. infectivity of prophage dna was associated with fast-sedimenting material of heterogenous size. infectious vegetative phage dna sedimented somewhat faster than mature phage dna; it was rapidl ...19704986850
threonine metabolism in a strain of bacillus subtilis. 19704986871
studies on the competence-inducing factor of bacillus subtilis.1. aqueous extracts of competent cells of bacillus subtilis 168i(-) were shown to contain a competence-inducing factor. the aqueous extracts were fractionated on deae-cellulose columns. 2. those fractions from deae-cellulose columns containing the competence-inducing factor were shown to exhibit a powerful lytic effect on isolated cell walls of b. subtilis 168i(-). little or no lytic activity was exhibited by the other fractions. 3. the kinetics of the lytic enzyme were investigated and found to ...19704986873
ethylene oxide penetration of the silicone coating used as a lubricant on disposable syringe rubber plunger tips and hypodermic needles. 19704986572
[international standardization of the biological activity of chlortetracycline]. 19704986658
studies of peptide antibiotics. xv. the synthesis of cyclo-(1-valyl-1-ornithyl-1-leucyl-d-phenylalanyl-1-prolyl-glycyl-glycyl). 19704988612
electron microscopy of loosened 50 s ribosomal subunits of bacillus subtilis. 19704988629
chemical modification of tyrosyl residues of the neutral protease obtained from bacillus subtilis var. amylosacchariticus. 19704988645
molecular basis of bacterial sporulation. 19704988654
rna polymerase mutants blocked in sporulation. 19704988656
structural alteration of rna polymerase during sporulation. 19704988657
sequential cleavage of dinucleotides from dna by sp3 dnase. 3. substrate specificity and initiation of the hydrolysis from the 5-terminus of polynucleotides. 19704988526
membrane synthesis in bacillus subtilis. i. isolation and properties of strains bearing mutations in glycerol metabolism. 19704988527
membrane synthesis in bacillus subtilis. ii. integration of membrane proteins in the absence of lipid synthesis. 19704988528
a study of the attachment phase of phagocytosis by murine macrophages. 19704988564
radio-sensitization with iodine compounds. i. examination of damage in deoxyribonucleic acid with bacillus subtilis transformation system by irradiation in the presence of potassium iodide. 19704988171
chromosomal location and properties of radiation sensitivity mutations in bacillus subtilis.transformation and transduction crosses involving reca1, recb2, and urv-1 mutations have shown that these mutations belong to three distinct unlinked genetic loci. the precise position of these loci on the bacillus subtilis chromosome map has been determined. the behavior of recb2 strains in transformation studies suggested a dominance of recb2(+) function over recb2 and an early expression of this phenotype during transformation. strains bearing two ultraviolet sensitivity markers possess a phe ...19704988238
cell wall polymers of bacillus sphaericus 9602. ii. synthesis of the first enzyme unique to cortex synthesis during sporulation.the cell wall peptidoglycan of vegetative cells of bacillus sphaericus 9602 contains l-lysine and d-isoasparagine and is devoid of diaminopimelic acid (dap), whereas the peptidoglycan of its spore cortex is devoid of l-lysine and d-isoasparagine and contains meso-dap. these two structures have a common biosynthetic precursor, uridine-diphospho-n-acetylmuramyl-l- alanyl-d-glutamic acid, which accepts either l-lysine or meso-dap, the latter reaction being the first unique to the synthesis of the s ...19704988239
cell wall binding properties of the bacillus subtilis autolysin(s).cell walls isolated from exponentially growing bacillus subtilis have autolysin(s) attached to them. an autolysin can be released from the walls by incubation at 0 c with 3 m licl. the enzyme can reattach to walls when the salt concentration is reduced. the bound enzyme cannot be removed or destroyed by washing the walls with 8 m urea at 0 c. the binding of free enzyme to walls at 0 c can take place normally in the presence of 2 m urea.19704988245
autolysin(s) of bacillus subtilis as dechaining enzyme.bacillus subtilis bacteria grow exponentially as long filaments in well-aerated cultures at 48 c. these filaments are chains of cells which can become unlinked when a b. subtilis autolysin or egg white lysozyme is added to the culture medium.19704988246
bacteriophage infection: which end of the sp82g genome goes in first?the transfer of deoxyribonucleic acid (dna) from bacteriophage sp82g to its host may be halted by chilling but is affected little by chloramphenicol, actinomycin d, or cyanide. the order of entry of markers on the phage genome was determined by halting the transfer of dna at intervals, removing the untransferred dna by blending, and assaying for the presence of markers in the blended complexes. markers on the phage genome are transferred in a linear, polar fashion consistent with the previously ...19704988268
characterization of inducible bacteriophages in bacillus licheniformis.two morphologically distinct and physically separable defective phages have been found in bacillus licheniformis nrs 243 after induction by mitomycin c. one of them (pblb) is similar to the defective phage pbsx of b. subtilis, which has a density of 1.373 g/cm(3) in cscl and a sedimentation coefficient of 160s. pblb incorporates into its head mainly bacterial deoxyribonucleic acid (dna) which has a sedimentation coefficient of 22s and a buoyant density in cscl of 1.706 g/cm(3). the other phage ( ...19704988269
[enzyme-substrate interaction in zone electrophoresis]. 19704988319
formylation of methionyl-transfer rna from prokaryotes and eukaryotes by bacillus subtilis transformylase. 19704988449
solubilization of dna in bacillus subtilis during recovery from uv-irradiation. 19704988476
[role of nicotinamide adenine dinucleotide dehydrogenases in initial phase of spore germination in bacillus subtilis]. 19704987262
problems in the bio-assay of orally administered cephaloglycin in biological fluids and method for the detection of its metabolite, desacetylcephaloglycin. 19704987304
the inactivation of bacillus subtilis alpha-amylase by n-acetylimidazole and tetranitromethane. 19704987312
ribonucleic acid sulfurtransferase from bacillus subtilis w168. sulfuration with beta-mercaptopyruvate and properties of the enzyme system. 19704987417
utilization of 14c-labelled bacillus subtilis and escherichia coli by sheep. 19704987422
synthesis of tyrocidine b1. 19704987425
a mutant of bacillus subtilis appearing to have a temperature sensitive defect in the ribosomes. 19704987432
low antibiotic potency of isohumulone.isohumulone is 15 to 30 times less inhibitory than lupulone or humulone on the growth of gram-positive bacteria.19704987202
subunit composition of b. subtilis rna polymerase. 19704987217
[isolation and properties of bacteriophage 2 c dna chains]. 19704986303
chemical derivatives of subtilisins with modified proteolytic activities. ii. succinyl- and glutarylsubtilisin type carlsberg. 19704987123
ribonuclease activity in the reticulocyte cell-free system. 19704987147
mode of action of siomycin. 19704984624
reduction in number of airborne bacteria by air cleaning devices in a closed space. 19704984148
inhibition of the amino acid induced initiation of germination of bacterial spores by chlorocresol. 19704984525
problems in purification of a bacillus subtilis autolytic enzyme caused by association with teichoic acid. 19704984551
[the flavonoids of propolis. isolation of a new bacteriostatic substance: pinocembrin (dihydroxy-5, 7 flavanone)]. 19704984610
the use of fluorescein isothiocyanate in the determination of the bacterial biomass of grassland soil. 19704984775
[protein-synthesizing systems of thermophilic and psychrophilic bacteria]. 19704984831
initiation of dna replication in bacillus subtilis. ii. linkage between termini of parental strands and origins of daughter strands. 19704984859
the 5'-ends of the dna of defective bacteriophages of bacillus subtilis. 19704984860
biochemical studies of bacterial sporulation and germination. 18. free amino acids in spores. 19704984698
biochemical studies of bacterial sporulation and germination. xix. phosphate metabolism during sporulation. 19704984699
biochemical studies of bacterial sporulation and germination. xx. phosphate metabolism during germination. 19704984700
antimicrobial polypeptide synthesized by mucor pusillus nrrl 2543. 19704985140
carbamyl phosphate biosynthesis in bacillus subtilis. 19704985262
hemocytes, hemolymph, and defense reactions in crustaceans. 19704985270
relationship between atmospheric temperature and survival of airborne bacteria.effects of temperatures ranging from -40 to 49 c on the behavior of airborne serratia marcescens, escherichia coli, and bacillus subtilis var. niger were investigated. aerosol decay rates of b. subtilis spores were not significantly affected by the temperature and remained approximately constant within the temperature range studied. the survival of airborne s. marcescens and e. coli was closely related to the temperature. an increase in temperature from -18 to 49 c resulted in a progressive incr ...19704985428
procedures for the assay of carbenicillin in body fluids.the assay of carbenicillin in clinical specimens is complicated by the fact that carbenicillin also contains a small amount of benzylpenicillin, thereby precluding the use of conventional penicillin assay organisms. this report gives details of a microbiological assay method involving the use of a strain of pseudomonas aeruginosa which is very sensitive to carbenicillin but insensitive to benzylpenicillin. the outline of a microassay method with this organism is presented, and a method for the a ...19704985430
enzyme induction in the tryptophan biosynthetic pathway in bacillus subtilis. 19704985442
effects of lysozyme on competence for bacillus subtilis transfection. 19704985443
defined conditions for dna extraction from bacillus subtilis spores. 19704984907
edeine. iv. structures of the antibiotic peptides edeines a1 and b1. 19704984939
penetration of gentamicin into the aqueous humor of human eyes. 19704984984
the quantitative measurement of dna hybridization from renaturation rates. 19704984993
the determination of molecular weight of bacterial genome dna from renaturation rates. 19704984994
[relations between physical-chemical properties and antimicrobial effect of beta-amino-ketones. 9. cytostatics]. 19704986468
muscle abscesses in children. 19704986501
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