Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| augmented immunogenicity of tumor cell membranes produced by surface budding viruses: parameters of optimal immunization. | membranes prepared from tumor cells infected with surface budding viruses are much more immunogenic than membranes from uninfected tumor cells. factors affecting immunization with membranes from virus-infected tumor cells were studied. preparations made with influenza virus were clearly superior to those prepared with vesicular stomatitis virus (vsv). membranes infected with vsv were maximally immunogenic at a dose equivalent to a 10% cell pack whereas influenza-virus-infected membranes were imm ... | 1976 | 182646 |
| inflammatory cells in solid murine neoplasms. i. tumor disaggregation and identification of constituent inflammatory cells. | mechanical and enzymatic methods of disaggregating tumors were studied with the goals of (1) minimizing cell losses while (2) maintaining functional and surface membrane markers needed to objectively identify inflammatory cells (ic)1 in resultant suspensions. application of the principles and methods described makes accurate estimation of the percentage of each ic type present in neoplasms possible for the first time. compared to purely mechanical means of disaggregating tumors, all enzyme mixtu ... | 1976 | 182647 |
| comparison of jc and bk human papovaviruses with simian virus 40: dna homology studies. | studies were performed to ascertain the relationship of human papovavirus jc to bk virus and to simian virus 40 (sv40) by further restriction endonuclease analysis and by dna-dna competition hybridization on membrane filters. form i dna extracted from two new isolates from cases of progressive multifocal leukoencephalopathy of human papovaviruses that were jc-like in their antigenic properties were found to yield restriction endonuclease fragmentation patterns similar to those of prototypic jc v ... | 1976 | 183019 |
| redistribution of immunoglobulin receptors on human neutrophils and its relationship to the release of lysosomal enzymes;. | release of the lysosomal enzyme beta-glucuronidase from human neutrophils was induced by igg or its fc fragment, aggregated by immune precipitation or by coating on latex particles. such release was inhibited when the cells were preincubated with free igg or fc fragments; f(ab')2 fragments were ineffective in both inducing and inhibiting beta-glucuronidase release. neutrophils incubated with igg or fc fragments, when challenged with anti-igg antibody, released lysosomal enzymes without the relea ... | 1976 | 183051 |
| increase of glucagon receptors in hyperthyroidism. | 1976 | 183145 | |
| cyclic amp and growth of ehrlich ascites tumor cells. lack of cyclic amp elevation in nutritionally deprived cells and mechanism of retardation of growth by dibutryl cyclic amp. | cyclic amp levels in ehrlich ascites tumor cells changed little after deprivation of cells of essential nutrients, serum, glucose and amino acids, deprival of each of which leads to marked inhibition of growth and protein synthesis. cyclic amp levels also changed little after the addition of these nutrients to deprived cells. thus cyclic amp is not likely to be the intracellular mediator for growth regulation by these three nutrients. elevation of cyclic amp levels for short periods by exposure ... | 1976 | 183828 |
| [adenylate cyclase system of the liver]. | 1976 | 183838 | |
| cytomegalovirus hepatitis in an artificial kidney unit. | serum hepatitis is a dreaded risk in connection with regular dialysis treatment (rdt). liver damage, however, can be cuased by other diseases, such as infection with cytomegalovirus (cmv). two cases in our artificial kidney unit revealed signs of liver damage with increased liver enzyme activity. case 1, a woman, was on rdt after an unsuccessful renal transplantation, and case 2, a man, belonged to the staff. serum hepatitis was initially suspected in both cases, but repeated examinations of the ... | 1976 | 184060 |
| effect of disinfectants on variola virus in cell culture. | twenty kinds of disinfectants were examined for ability to inactivate variola virus. cytopathic effect and plaque formation on monolayer cultures of an established monkey kidney cell line were used as indicators of virus inactivation. a micromethod using microplate cultures, and not requiring a co2 incubator, was adopted. the procedures were straightforward, showing good reproducibility. among the compounds tested, several were found to be superior because of the minimum concentrations required ... | 1976 | 184735 |
| surface antigens on hela cells persistently infected with hvj (sendai virus). | surface antigens of helahvj cells, a cell line persistently infected with hvj, were studied by fluorescent antibody staining. after absorption with concentrated hvj virions and hela cells, anti-helahvj antiserum was able to demonstrate specific surface fluorescence on helahvj cells, while this serum no longer reacted with original hela cells nor with hvj virions. during cytolytic infection of hela cells with hvj, this specific surface antigen appeared at an early stage of infection prior to the ... | 1976 | 184762 |
| a cyclic adenosine 3':5'-monophosphate-mediated effect of cholera toxin on high-molecular-weight glycoprotein species of malignant cells. | a comparison of the pronase-sensitive glycosylated species detectable under permissive and nonpermissive conditions by normal rat kidney cells transformed by a temperature-sensitive derivative of rous sarcoma virus reveals relative decreased labeling of high-molecular-weight glycosylated species under conditions that allow the expression of transformation, in medium supplemented either with 0.5% calf serum or with human alpha2-macroglobulin, 100 mug/ml. exposure of the cultures to cholera toxin ... | 1976 | 184943 |
| msh stimulates adenylate cyclase and tyrosinase in cultivated melanoma cells in the presence of cytochalasin b. | 1976 | 185075 | |
| the syrian hamster: a reproducible model for studying changes in intestinal fluid secretion in response to enterotoxin challenge. | syrian hamsters respond in a predictable and reproducible manner to intragastric administration of purified cholera enterotoxin by intraluminal accumulation of fluid in the small bowel, cecum, and proximal colon. in the majority of animals this process is self limiting, and recovery occurs with full reabsorption of intestinal fluid by 30 to 35 h. the secretory response to 75 mug of cholera toxin has been defined, and the model was utilized to study the inhibitory effects of indomethacin, polymyx ... | 1976 | 185149 |
| biosynthesis and function of gangliosides. | gangliosides are unique acidic glycolipids that are selectively concentrated in the plasma membrane of cells. surface labeling studies have demonstrated that at least a portion of the oligosaccharde chain of gangliosides extends beyond the hydrophe) is imbedded in the membrane bilayer. it is becoming increasingly apparent that gangliosides participate in the internalization of environmental signals elicited by cholera toxin and glycoprotein hormones such as thyrotropic hormone and chorionic gona ... | 1976 | 185697 |
| stimulation of adenylate cyclase in washed pigeon erythrocyte membrane with cholera toxin and its subunits. | 1976 | 185963 | |
| subunit structure of cyclic gmp-dependent protein kinase from guinea pig fetal lung: dissociation of holoenzyme by cyclic gmp and histone. | 1976 | 186069 | |
| [effect of propranolol and trimepranol on the activity of carboxylic esterase, acid and alkaline phosphomonoesterases and adenosinetriphosphatase in the human thyroid gland]. | 1976 | 186198 | |
| the activation of adenylate cyclase by cholera toxin: possible interaction with the nucleotide regulatory site. | the application of cholera toxin to intact cells causes a stimulation of adenylate cyclase activity. the effect is characterized by a lag period followed by a progressive rise in enzyme activity over several hours. only a few minutes' exposure to the toxin is required to produce effects lasting over several days. stimulation of adenylate cyclase by cholera toxin in broken cell preparations requires the presence of nicotinamide-adenine dinucleotide (nad) and an unidentified component of the cytos ... | 1976 | 186240 |
| adenylate cyclase in foetal rat testes and its stimulation by lh and naf. | 1976 | 186308 | |
| cyclic amp control by prostaglandin e1 in non-parenchymal liver cells. | 1976 | 186314 | |
| pancytopenia and "caillary leak syndrome" with infectious mononucleosis. | a 19-year-old man with documented infectious mononucleosis presented with pancytopenia and a megaloblastic bone marrow. he developed a "capillary leak syndrome" with an expanded plasma volume of 9,290 ml and normal right heart and pulmonary artery pressures. the patient had a dramatic recovery after corticosteroid therapy. | 1976 | 175508 |
| increased cyclic adenosine 3',5'-monophosphate content in guinea pig ileum after exposure to staphylococcus aureus delta-toxin. | to compare staphylococcus aureus delta-toxin with cholera toxin, which is known to increase cellular cyclic adenosine 3',5'-monophosphate (camp), studies were undertaken to determine the effect of delta-toxin on the camp content of guinea pig ileum maintained in vitro. concentrations of delta-toxin as low as 0.40 mug/ml increased camp levels in guinea pig ileum after 2 h of incubation. histological damage was seen in ileum exposed for 2 h to delta-toxin concentrations of 100 mug/ml. as little as ... | 1976 | 175015 |
| immunological properties of bovine serum lipoproteins and chemical analysis of their protein moieties. | four classes of bovine serum lipoproteins were isolated by precipitation with dextran sulfate, ultracentrifugation, and preparative electrophoresis on polyacrylamide gel. very low density lipoprotein (d less than 1.019 g/ml) was related immunologically to low density lipoprotein-two (d 1.039 to 1.060 g/ml) and high density lipoprotein (d 1.060 to 1.210 g/ml) was related immunologically to low density lipoprotein-one (d 1.019 to 1.039 g/ml), but the two pairs were immunologically distinct. the ma ... | 1976 | 175102 |
| ultrastructure of mycoplasma-like organisms purified from clover phyllody-affected plants. | 1976 | 175171 | |
| translation of oncogenic virus rna in xenopus laevis oocytes. | 1976 | 175294 | |
| interaction of ultrasound with neoplastic tissue. ii. systemic effects after local sonic irradiation. | local sonic irradiation was applied to subcutaneously implanted furth-columbia rat wilms' tumor. the weight and the rate of tritiated thymidine uptake were measured in host organs distal to the application field. kidney and spleen weights were inhibited by the wilms' tumor, and sonication of the tumor removed all or part of this inhibition. liver weight was increased after sonication of tumor-bearing rats but not in nontumor-bearing rats. this may have been a response to tumor-specific substance ... | 1976 | 174264 |
| association of herpes simplex virus type 1 dna with host chromosomal dna during productive infection. | 1976 | 174293 | |
| effect of estrogen on gene expression in the chick oviduct. correlation between nuclear-bound estrogen receptor and chromatin initiation site for transcription. | the [3h]estradiol exchange assay was used to characterize the nuclear estrogen receptor from the chick oviduct. after diethylstilibestrol (des) treatment (14 days), the oviduct nuclei contained estrogen receptors that manifested high affinity (kd = 2 x 10(-9)m) and low capacity binding (4000 to 5000 sites/cell) for estradiol. des and estradiol competed significantly for [3h]estradiol binding, while testosterone and progesterone were ineffective. these binding sites were found in the oviduct and ... | 1976 | 173720 |
| antibody-dependent cell-mediated cytotoxicity to target cells infected with type 1 and type 2 herpes simplex virus. | the phenomenon of antibody-dependent cell-mediated cytoxicity (adcc) has been extended to include target cells acutely infected with herpes simplex type 1 virus (hsv-1) or herpes simplex type 2 virus (hsv-2) in an in vitro system that employs immune human serum and human blood mononuclear cells. the cytotoxic reaction was detectable after 1 hr of incubation and was complete between 4 and 8 hr. the amount of adcc noted was directly proportional to the logarithm(10) of the effector: target cell ra ... | 1976 | 173757 |
| effects of chronic uremia, hemodialysis, and renal transplantation on plasma lipids and lipoproteins in man. | since quantitative and qualitative alterations in plasma lipoproteins may provide insights into mechanism(s) of altered lipid transport in renal failure, whole plasma triglyceride (tg) and cholesterol (chol) concentrations and lipoprotein neutral lipids and composition were examined in patients with chronic renal failure (undialyzed and dialyzed) and following successful renal transplantation. both uremic groups demonstrated increased tg (p less than 0.001) and normal chol in whole plasma and in ... | 1976 | 173766 |
| activation of renal erythropoietic factor by phosphorylation. | renal erythropoietic factor (ref) serves as a substrate for a cyclic nucleotide-dependent protein kinase. the phosphorylation of ref is associated with increased erythropoietic activity. conversely, ref isolated from hypoxic rats is a poor phosphate acceptor. these findings and the presence of a positive correlation between erythropoietin levels and urinary excretion of adenosine 3',5' monophosphate (cyclic-amp) in anemic individuals suggest that the cyclic-amp-protein kinase system plays an imp ... | 1976 | 173768 |
| [age dependent frequency of cytoplasmic antibodies]. | in a random sample of so-called healthy blood donors under and over 60 years of age (n=196 of 10,593 donations by about 4254 donors) the frequency of antibodies (antibodies against smooth muscles, mitochondria, microsomes, brush border of proximal rat renal tubuli, connective tissue, and parietal cells) detectable by immuno-fluorescence was determined and compared with the incidence thereof in various groups of diseases. in general, two frequency peaks (up to 30 and over 50 years of age) were ob ... | 1976 | 139091 |
| dynamics of sulfonylurea-induced insulin release from the isolated perfused rat pancreas. | in the isolated perfused rat pancreas various sulfonylurea drugs were tested with a basal glucose level of 1 mg/ml in the perfusion buffer and were found to cause a biphasic insulin response. novocs 476, a new and potent sulfonylurea, and glibenclamide qualitatively differed from tolbutamide, glibornuride, glipizide, and glisoxepide, which were all alike in terms of the relationship between first and second phases of insulin release. | 1976 | 134920 |
| [bdellovibrio bacteriovorus as a factor in the self-purification of river water]. | 1976 | 134929 | |
| dysplasia of the tricuspid valve in the dog and cat. | dysplasia of the tricuspid valve in 14 dogs and 13 cats was studied. the clinical, electrocardiographic, radiographic, hemodynamic, angiocardiographic, and pathologic findings were reviewed in each species. alterations of the tricuspid valve complex included long, thick septal leaflets adhered to the septum; absent or short, stout fused chordae tendineae; hypertrophic fused papillary muscles; insertion of papillary muscles directly into the lateral leaflets; incomplete development of the valvula ... | 1976 | 134984 |
| [on the problem of atypical dysentery bacilli (author's transl)]. | 1976 | 135237 | |
| transplantation of hymenolepis diminuta into naive, immune and irradiated mice. | almost 100% of 7- to 10-day-old hymenolepis diminuta became established when surgically transplanted from donor mice into the duodenum of naive recipient mice. transplanted tapeworms survived 8-12 days, by which time they had survived much longer in total than they would have done in the donor. mice previously immunized by a primary infection rejected transplants within 4 days. sub-lethal irradiation (550 rad) delayed rejection by immune mice but such mice still rejected worms much more quickly ... | 1976 | 135242 |
| liver plasma membrane enzyme activities following glutaraldehyde fixation. | the wachstein-meisel atpase histochemical method has been previously used to demonstrate the ultrastructural localization of this enzyme in both whole liver and isolated plasma membranes following fixation in glutaraldehyde. in the present study biochemical assay, of liver plasma membrane enzymes following fixation in cold 2.5% glutaraldehyde showed that approximately 40% of mg2+-atpase, but only 4% of (na+-k+)-atpase activity remained in membranes from either control or anit-treated rats. in ad ... | 1976 | 135243 |
| cerium nitrate: a new topical antiseptic for extensive burns. | the wounds of 60 burned patients were treated topically with cerium nitrate, which was applied either as a cream or in aqueous solution. cerium nitrate has a potent antiseptic effect in human burn wounds, especially against gram negative bacteria and fungi. pseudomonas aeruginosa was recovered from the wounds infrequently and never predominated. fungi were practically never found. no patient treated with cerium developed a necrotizing wound infection. analysis of the detailed bacteriological dat ... | 1976 | 135364 |
| suppressor mechanisms in neonatally acquired tolerance to a gross virus-induced lymphoma in rats. | tolerance to a highly immunogenic gross virus-induced tumour in wistar/furth rats (c58nt)d was produced by neonatal infection of the rats with the virus. these rats failed to reject the tumours when challenged 8 weeks after virus inoculation and to mount the appropriate cell-mediated immune response to the tumour. the mechanisms involved were studied in vivo by adoptive transfers into sub-lethally irradiated rats of tumour cells mixed with spleen cells and/or sera from normal, tolerant, or tumou ... | 1976 | 135385 |
| effect of testosterone administration on the epiphyseal cartilage of hypophysectomized rats. | for the study of the mechanism of action of testosterone histological, carbohydrate-, and enzyme histochemical investigations were carried out on the epiphyseal cartilage of (1) hypophysectomized rats treated with testosterone [(thx group); (2)] hypophysectomized rats without hormonal treatment (hx group), and (3) intact, untreated control rats. the results were compared with the data obtained in a previous experiment in which intact rats were treated with testosterone (t group). the experiments ... | 1976 | 135467 |
| effect of vibrio cholerae neuraminidase on the generation of cell-mediated cytotoxicity in vitro. | vibrio cholerae neuraminidase (vcn))12.5 units/2 x 10(6) cells/ml) continuously present for a standard 5-day mlc will significant (p less than 0.02) increase the cytotoxic activity generated by a given number of responding spleen cells without reducing the specificity. heat-inactiviated vcn produced no such augmentation. this augmented cytotoxicity could be reproduced by preincubating (1 hr) the responding spleen cells with vcn (25 units/2.5 x 10(6) cells/ml) before addition of stimulating splee ... | 1976 | 136474 |
| tobacco, auxiliary etiologic factor of cancer of the larynx and hypopharynx]. | 1976 | 136491 | |
| induction of an autologous immune-complex glomerulonephritis in the rat by intravenous injection of heterologous anti-rat kidney tubular antibody iv: effect of injection of hgcl2 prior to the antibody. | autologous immune-complex glomerulonephritis developed in rats injected s.c. with hgcl2 2 days before the injection of anti-tubular fraction 3 antibody. the glomerulonephritis was progressive and characterized by granular deposition of igg and c-3, with proteinuria from the eighth week onwards. granular densities and severe glomerular basement membrane changes were observed when the experiment was terminated after 9 months. a possible mechanism of the glomerular lesion is discussed. | 1976 | 136979 |
| [reversal of the depressive power of cyclophosphamide on the anti-infectious defense of the mouse by means of a somatic antigen from bacillus subtilis]. | in the experimental conditions reported the cyclophosphamide increases the pathogenic effect of escherichia coli in mice. treating the animals with a somatic antigen of bacillus subtilis, reverses the aggravating effect of cyclophosphamide on the experimental infection. similar results are obtained through the parenteral and the rectal routes. this antigen does not limit either the leucopenient effect of cyclophosphamide nor its blocking effect on the synthesis of sheep red blood cells antibody ... | 1976 | 137039 |
| hyperkeratosis of the palms and soles associated with internal malignancy and elevated levels of immunoreactive human growth hormone. | 1976 | 137091 | |
| studies of the histochemistry, ultrastructure, motor innervation, and regeneration of mammalian intrafusal muscle fibres. | 1976 | 137430 | |
| [newly detected relationships between antibacterial activity and structure of some chalocones]. | 1976 | 130785 | |
| crosslinking of membranes: the effect of dimethylsuberimidate, a bifunctional alkylating agent, on mitochondrial electron transport and atpase. | 1976 | 130833 | |
| cleavage of thrombosthenin a by thrombin. evidence for the existence of two types of bovine platelet actin. | bovin platelet actin prepared by spudich's method (spudich, j. a. (1972) cold spring harbor symp. quant. biol. 27, 585-594) separated into two peaks on a sephadex g-200 column. the actin of both peaks had a mol. wt. of 42 000 on sodium dodecyl sulfate-polyacrylamide gel and activated myosin atpase, although in a quantitatively different manner. actin eluted in the first peak (probably an oligomeric form) was not polymerized in 2 mm mgcl2 and 0.05 m kcl, while that of the second peak went through ... | 1976 | 130929 |
| [spontaneous peripheral proteolysis/1st communication: method and results of clinical examinations (author's transl)]. | with a modificated astrup-fibrin plate-method also an inhibition of proteolysis can be registrated. in various medical areas a spontaneous peripheral proteolysis had been found, especially so in chronical bacterial infections. | 1976 | 132940 |
| [study of the incorporation of radioactive zinc in saccharomyces cerevisiae aldolase]. | saccharomyces cerevisiae aldolase concentrates from the culture medium containing znso4 a large amount of zn which becomes a component part of the enzyme molecular structure. this was made evident by adding to the culture medium 65znso4 and measuring the radioactivity of the aldolase extracted by a phillips single channel analyzer. | 1976 | 133445 |
| evidence of a (na+, k+) atpase activity in human circulating lymphocytes. | 1976 | 134750 | |
| [afferent projections to the locus coeruleus nucleus in the cat. study by the horseradish peroxidase technic]. | using a retrograde tracer technique with horseradish peroxidase, we have revealed some afferent projections to the locus coeruleus complex from the contralateral pontine tegmentum, raphe nuclei, substantia nigra, nucleus of the solitory tract, dorsal motor nucleus of the vagus and other regions of the ponto-bulbar reticular formation as well as from hypothalamic and preoptic areas. | 1976 | 134760 |
| [turnover of free adenine nucleotides of the isolated and perfused rabbit heart]. | the turnover of free adenine nucleotides was studied in the perfused rabbit heart by the decline in radioactivity 3 h 30 after administration of a single pulse of tritiated adenine. the radioactivity of the perfusate was followed during the experiments. about 7% of the labelled adenine nucleotides were renewed by hour and 1,5% of the radioactivity was lossed by hour in the perfusion fluid. | 1976 | 134762 |
| [motricity of the female genital apparatus in mammals. hormonal correlations]. | the motility and the pharmacological reactivity of the uterine tract of mammals are linked with the ovarian cycle and the gestation. in the birds, the study of general scheme of the hormonal mechanism and the uterine motility involued in the ovulation, the egg formation and the uterine dilatation can be used as an experimental model of the mammalian gestation. | 1976 | 134763 |
| [influence of microflora on the metabolism of l-threonine at the level of the rat digestive tract]. | intestinal tissues are found to incorporate l threonine 14cu at faster rate in the absence of microflora. most of the radioactivity of the digestive contents occurs in the tca soluble compounds, except in the coecum and large intestine of the conventional rats where the microflora synthetized its own proteins from the labelled digestive material. free threonine is the main labelled soluble compound in the conventional rats digestive contents. in the other hand, in the germfree rats digestive con ... | 1976 | 134804 |
| [blood on filtration paper for determination of rubella haemagglutination-inhibiting antibodies (author's transl)]. | 1976 | 134837 | |
| glucocorticoid and starvation effect on glycosaminoglycans in vascular connective tissue. biochemical studies on repair processes in rabbit aorta. | male rabbits were injured by a single mechanical dilatation injury of aorta and then injected with prednisone 2 mg/kg or saline for 14 days or subjected to starvation. the biosynthesis of the sulfated glycosaminoglycans as evaluated by the uptake of 35s-sulfate and the content of the glycosaminoglycans were measured on the intima-media layer of the descending thoracic aorta. the results indicate that prednisone may inhibit the biosynthesis of heparan and/or dermatan sulfate while starvation incr ... | 1976 | 134864 |
| a stabilised tris(hydroxymethyl)aminomethane adduct in reduced collagen. | the reduction of collagen with sodium [3h] borohydride in the presence of tris buffer results in the stabilization of a schiff base adduct which is formed between allysine residues and tris(hydroxymethyl)aminomethane. the reduced, radioactive derivative of this adduct has been identified in hydrolyzates or reduced collagen. it elutes before hydroxylysine on an amino acid analyzer column close to the position of dihydroxylysinonorleucine. similar artifacts may occur when aldehydes present in or a ... | 1976 | 134871 |
| the subunits of rabbit-muscle phosphofructokinase. a search for sequence repetition. | rabbit muscle phosphofructokinase uniformly carboxymethylated with iodo[2-14c]acetate consists of subunits with a molecular weight of 80 000 +/- 5000. the subunit polypeptide chain contains 16 and 52 residues respectively of cysteine and arginine and, contrary to previous results, peptide mapping experiments gave no indication that phosphofructokinase chains yield fewer than the expected numbers of cysteine and arginine containing peptides. to test further for the possible occurrence of repeat s ... | 1976 | 134894 |
| cryopreservation of lymphocytes for use in in vitro assays of cellular immunity. | human lymphocytes can be cryopreserved for the purpose of performing in vitro tests of cellular immunity. a systematic study of the conditions for freezing and recovering cells has shown that there is wide flexibility in cell concentrations of cryoprotective agent and the temperature and rate of dilution however, have definable optima. cells frozen and thawed under optimal conditions retain their reactivity in mlc and can be used for sequential studies of immune responsiveness. approximately 70% ... | 1976 | 130427 |
| differential mechanisms of increased alpha 1-fetoprotein production in rats following carbon tetrachloride injury and partial hepatectomy. | possible differences in the mechanisms of increased alpha1-fetoprotein (afp) production following carbon tetrachloride (ccl4) intoxication and partial hepatectomy were studied with 5-week-old rats at the time of sacrifice. the maximum level of serum afp reached in 4 days after a single dose of ccl4 was much higher than that after partial hepatectomy, although the incorporation of [3h]thymidine into liver dna increased nearly to the same extent by either of these treatments. in the remnant after ... | 1976 | 58715 |
| induction of tryptophanase in nitrofurantoin resistant vibrio el tor. | 1976 | 59523 | |
| amino acid metabolism and protein synthesis in streptomycin resistant vibrio el tor. | metabolic activities in relation to protein synthesis and amino acid utilization are altered in vibrio el tor after development of resistance towards streptomycin. efficiency of in vivo and in vitro protein synthesis is markedly reduced in streptomycin resistant vibrio el tor. the rate of incorporation of 14c-amino acids into protein, uptake of 14c-valine and oxidation of certain amino acids are also altered. | 1976 | 59524 |
| small fragments from the a subunit of cholera toxin capable of activating adenylate cyclase. | exposure of cholera toxin to membrane particles prepared from sarcoma 180 cells gives rise to a variety of fragments which are capable of activating adenylate cyclase [atp:pyrophosphate-lyase (cyclizing), ec 4.6.1.1]. a major component of these fragments has an apparent molecular weight in the 8,000-10,000 range. the smallest stimulatory fragment has a molecular weight of approximately 1400. the small size of the fragments is confirmed by sephadex gel filtration, in the presence of either sodium ... | 1976 | 60760 |
| [production and use of a cholera antigenic erythrocyte diagnostic agent]. | 1976 | 60852 | |
| macrophage plasminogen activator: modulation of enzyme production by anti-inflammatory steroids, mitotic inhibitors, and cyclic nucleotides. | plasminogen activator production by cultured mouse peritoneal macrophages can be modulated in vitro by low concentrations of various pharmacologically active molecules. glucocorticoid hormones and their synthetic derivatives, as well as cholera toxin, colchicine, and vinblastine markedly inhibit production of this enzyme without affecting other important macrophage functions. the effect of glucocorticoids is of particular interest, both because their relative in vivo anti-inflammatory potencies ... | 1976 | 61067 |
| reverse plaque formation by hog cholera virus of the gpe-strain inducing heterologous interference. | a simple and rapid plaque procedure was developed for the assay of hog cholera virus (hcv) of a particular strain, gpe-, based on its intrinsic interference with vesicular stomatitis virus (vsv) on the primary swine testicle cells and on an established swine kidney cell line; the procedure is called the reverse plaque formation (rpf) method. the plaques were produced as colonies of hcv-infected cells which were vsv-sensitive, disintegrated cell sheet. these plaques became visible after 15 to 20 ... | 1976 | 61176 |
| [rapid method of determination of sensitivity of vibrio cholerae to antibiotics]. | 1976 | 61330 | |
| is cholera primarily water-borne. | 1976 | 62178 | |
| cholera, copepods, and chitinase. | 1976 | 62179 | |
| [antigenic and serologic properties of yersinia enterocolitica]. | 1976 | 62475 | |
| cholera is primarily waterborne in bangladesh. | 1976 | 63781 | |
| [the effect of increased intracranial pressure and subarachnoid hemorrhage on the sympathetic vasoconstrictor nerve activity (author's transl)]. | 1976 | 64942 | |
| [methodical recommmendations for conduction of the hemagglutination test with erythrocytic cholera enterotoxic diagnostic agent (eceda)]. | 1976 | 66381 | |
| experimental cancer immunotherapy: modification of tumor cells to increase immunogenicity. | firmly established transplantable c3h/hej mammary carcinomas can be inhibited by host challenge with vibrio cholerae neuraminidase (vcn)-treated tumor cells. the effect is totally immunospecific, even vcn-treated tumors bearing shared mammary tumor virus (mtv) antigen cannot induce the regression. thus, vcn is capable of increasing the immunogenicity of the private, unique-unshared tumor antigens on mammary carcinomas; vcn is incapable of increasing the immunogenicity of the shared mtv-associate ... | 1976 | 68699 |
| a serial study of pregnancy proteins in primigravidae. | the plasma concentrations from four 'pregnancy proteins' and three steroid hormones have been measured throughout pregnancy in 15 primigravidae. two of the proteins, human placental lactogen (hpl) and pregnancy-specific beta1-glycoprotein (psbetag), are specific for pregnancy and correlate well with the stage of gestation. it is suggested that measurement of psbetag may be useful in assessing feto-placental wellbeing. neither of the pregnancy-associated proteins, steriod-binding beta-globulin (s ... | 1976 | 57799 |
| fetal syphilis in the first trimester. | evidenc of first-trimester fetal syphilis was sought in the products of conception in a therapeutic abortion clinic. during two collection periods of one week, five patients with serologic and clinical data consistent with recent syphilitic infection were identified. their conceptuses were carefully examined by silver and immunofluorescent stains for the presence of treponema pallidum. two of these five conceptuses (9 and 10 weeks' gestation) were found to contain t. pallidum by these methods. t ... | 1976 | 56895 |
| [ultrastructural analysis of preparations impregnated with silver salts]. | the work was devoted to the ultrastructural analysis of the neurohistological preparations. sections of the tissue from the precardial parts of the pulmonary and caval dog veins were impregnated with silver salts after campos and embedded in the araldite by a special method. electronmicroscopi studies showed reduced silver adsorbed by the tissue of the impregnated preparations to exhibit a granular structure (the granules were 30-400 a in size). the largest of them were revealed in the axoplasm ... | 1976 | 55281 |
| interaction of cholera toxin and toxin derivatives with lymphocytes. ii. modulating effects of cholera toxin on in vivo humoral and cellular immune responses. | the in vivo effects of cholera toxin on lymphoid organ structure and function in mice were investigated. it was found that within a day following intravenous injection of 1 mug of toxin, thymus as well as spleen weight decreased but the animals remained healthy. histological studies suggested that the involution of lymphoid organs was due to cell death. injection of cholera toxin into adrenalectomized mice was lethal within 36 h. in these animals no decrease in lymphoid organ weight was noted. t ... | 1976 | 5378 |
| comparative study of the proteinograms of vibrios and of closely related microorganisms by means of polyacrylamide gel disc electrophoresis. | proteinograms of 112 strains of vibrios and closely affiliated microorganisms were studied by disc electrophoresis in polyacrylamide gel. up to 25 protein peaks with definite mobility coefficients were revealed. the influence of the culture medium on the protein spectrum of the microbes was found. the frequency of peak formation was of great significance for the differentiation of the microbes under study. the quantitative characteristics of the peak area could not be used for differentiation. | 1976 | 7908 |
| molecular weight analysis of the polypeptides of cholera phage pl 163/10 by polyacrylamide gel electrophoresis. | cholera phage pl 163/10, belonging to mukerjee's group i, was purified by alternate cycles of low and high speed centrifugation. gel electrophoresis revealed the presence of four polypeptide chains of respective molecular weights of 10,370+/-515 (a), 30,000+/-1,303 (b), 40,000+/-1,049(c) and 64,000+/-2,433 (d) daltons. electrophoresis of the sample alkylated with iodoacetic acid resolved the presence of only one polypeptide chain of an average molecular weight of 10,310+/-565 daltons. the polype ... | 1976 | 9816 |
| use of thyrotropin and cholera toxin to probe the mechanism by which interferon initiates its antiviral activity. | thyrotropin (10 mum) inhibited the antiviral activity of interferon. when added after interferon, thyrotropin (tsh) had no effect on antiviral activity. there was also no inhibition of interferon action in cells washed with medium between incubations with tsh and interferon. 125i-labeled tsh and 125i-labeled cholera toxin could bind to preparations of mouse l-cell plasma membranes. the binding was specific in that it was prevented by unlabeled thyrotropin or cholera toxin, but not by insulin, gl ... | 1976 | 10573 |
| antibacterial activity of ambodryl and benadryl. | 1976 | 11202 | |
| effect of caffeine on the motility, viability, oxygen consumption and glycolytic rate of ejaculated human normokinetic and hypokinetic spermatozoa. | in this report the effect of caffeine, ibmx and cholera toxin on ejaculated human spermatozoa was tested. it was found that caffeine was the most effective drug, and that cholera toxin was ineffective. caffeine stimulated motility, preserved viability and increased the glucose, fructose utilization and lactate production of sperm in whole semen and washed sperm. oxygen consumption however was decreased. these results of the normokinetic ejaculates were defined as a basis for comparison with the ... | 1976 | 12119 |
| gas levels and biochemical changes in cerebrospinal fluid after experimental brain injury: its effect on cerebral circulation and cerebral metabolism as well as its management. | 1. after brain damage experimentally induced by balloon compression, liquor of the cistern shows a metabolic acidosis with significantly increased lactate level and l/p quotient, and statistically distinct decrease of ph, oxygen tension, and bicarbonate level. 2. there is a considerable disproportion between the changes of acid-base metabolism, gas tension, and metabolic products in cerebrospinal fluid compared with much less expressed disturbances in the blood. 3. the advanced edematous phase s ... | 1976 | 15353 |
| effect of cholera enterotoxin preparations on cutaneous response in rabbit under varied conditions. | enterotoxic activity of two preparations obtained from vibrio cholerae, b-53-6 inaba and b-53-10 ogawa was tested in ligated ileal loops of rabbit. the biologically active enterotoxic preparations were further used to study the permeability reaction in rabbit skin. cutaneous response tended to be linear only with higher concentrations of the toxin and showed maximum blueing intensity between 16 and 24 hrs of intracutaneous inoculation. exposure of enterotoxin preparations to elevated temperature ... | 1976 | 13591 |
| the arrangement of subunits in cholera toxin. | cholera toxin consists of five similar b subunits of apparent molecular weight about 10 600 and one a subunit (29 000) consisting of two peptides (a1 23 000-24 000 and a2 about 5500) linked by a single disulfide bond. each b subunit also contains one internal disulfide bond which is readily reduced but is protected from carboxymethylation unless the reduced subunits are heated in urea. tyrosine residues in a1 and in b subunits are readily iodinated, but the intact b assembly does not react with ... | 1976 | 3214 |
| properties of cholera toxin- and naf-stimulated adenylate cyclase from mouse thymocytes. | kinetic parameters of mouse thymocyte adenylate cyclase activity were determined. naf and cholera toxin stimulated adenylate cyclase. stimulation by either agent did not change the ph or mg2+ optima relative to control (unstimulated cyclase). the km value for atp of adenylate cyclase stimulated by naf was significantly reduced from control. by contrast, cholera toxin treatment did not change the km relative to control. adenylate cyclase, when stimulated by naf, had an optimum for mn2+ alone, or ... | 1976 | 3217 |
| secretory iga against enterotoxins in breast-milk. | a pool of colostrum from guatemalan mothers (guatemalan colostrum)) obtained 2-4 days post partum inhibited the induced fluid accumulation in rabbit ileal loops when incubated with vibrio cholerae or escherichia coli enterotoxin. there was a linear relationship between the quantity of colostrum used and the protection achieved. pools of guatemalan breast-milk obtained 15-30 days post partum and north american breast-milk had the same effect when tested with e. coli and v. cholerae enterotoxins, ... | 1976 | 73692 |
| effect of neurotensin, substance p and morphine sulfate on the secretion of prolactin and growth hormone in the rat. | neurotensin (nt), substance p (sp) and morphine sulfate (ms) elevate plasma prolactin and growth hormone levels in both normal or estrogen-progesterone pretreated male rats. by contrast, steroid priming is required for trf to exhibit prl-releasing activity. naloxone, an opiate receptor blocker, reverses the stimulatory effect of ms only. diphenhydramine, a histamine antagonist, inhibits the response to nt, sp and ms without affecting the response to trf. these results suggest the involvement of ... | 1977 | 95701 |
| group f, a new vibrio? | 1977 | 95779 | |
| the response of experimental animal tumors to neutron radiation therapy. | 1977 | 96056 | |
| a limited serological survey of north island cattle for antibody to a number of endemic viruses. | 1977 | 96390 | |
| hemostasis in schistosomiasis mansoni. | hemostasis was studied in 18 patients with the hepato-intestinal form of schistosomiasis mansoni and in 23 with the hepato-splenic form. both groups are compared referring to alterations found. the relations between plasmatic coagulation factors and hepatocytic function tests are studied. in 6 patients the explorations were repeated after administration of hycanthone. in the patients with the hepato-splenic form, the presence of chronic consumptive coagulophathy was found. the coagulopathy disap ... | 1977 | 97124 |
| [treatment of acute pancreatitis by glucagon. experimental studies in dogs]. | the authors made a comparative study of the use of glucagon (4 mg/day iv) in 30 dogs with acute pancreatitis. they found that the mortality and amylasemia were significantly lower in the glucagon-treated group than in the control groups (saline and glucose solution 5%). in the glucagon group, the areas of necrosis were smaller (1.5 cm) and rarely found; microscopically, the areas of necrosis and the inflammatory reaction were much smaller than in the other groups. these findings lead to the conc ... | 1977 | 97132 |
| [serological survey of animal rickettsioses in cameroon. i. evaluation of the threshold of positivity and specificity of the micro-agglutination reaction as related to the size of the animal]. | 1977 | 96947 | |
| tropical diseases. | 1977 | 97727 | |
| biochemistry and immunochemistry of membrane-bound enzymes. | membrane-bound enzymes have certain specific differences compared with soluble enzymes. membrane-binding often enables greater catalytic activity of associated enzymatic reactions, their regulation by low molecular weight substances (substrates and allosteric effectors, hormones) and compartmentation, etc. on the other hand, the binding of enzymes to membranes causes considerable difficulties as regards their isolation and the determination of their homogeneity and substrate specificity. membran ... | 1977 | 97930 |