Publications

the a- and b-chains of carboxypeptidase i from germinated barley originate from a single precursor polypeptide.	carboxypeptidase i from germinated barley (hordeum vulgare) grain consists of two peptide chains linked by disulfides; the a- and b-chains contain 266 and 148 amino acid residues, respectively (sorensen, s. b., breddam, k., and svendsen, i. (1986) carlsberg res. commun. 51, 475-485). a cdna library prepared from mrna isolated from scutella of 2-day germinated barley has now been screened with a mixed oligonucleotide encoding a peptide fragment of the a-chain. nucleotide sequence analysis of a 14 ...	1988	3403516
evolution of plague virulence.		1988	3405295
effect of pregnancy on ritodrine pharmacokinetics.	dosing regimens for ritodrine are based in large part on pharmacokinetic studies performed with nonpregnant subjects. pregnancy is characterized by changes in renal blood flow, plasma volume, protein concentration, and hepatic function. these physiologic changes frequently alter drug pharmacokinetics. to define the effect of pregnancy on ritodrine kinetics, we compared ritodrine pharmacokinetics in four pregnant and four nonpregnant rhesus monkeys. significant differences were demonstrated in th ...	1988	3407687
expression of fibronectin on clonally related transformed and control sublines from an epithelial cell strain and a tumor line of mouse alveolus.	the cellular expression of fibronectin and the importance of fibronectin for the morphology of cultured cells were studied in a lung epithelial cell system. the cells used were clonally related control nal1a, spontaneously transformed nal1as cells of the nal1a cell strain, and transformed clonal sublines of the cell line nal1am and the tumor cell line nul1. fibronectin was detected on the surface of nal1a cells by surface iodination; fibronectin synthesis, secretion, and pericellular accumulatio ...	1988	3409226
low-frequency ultrasound absorption in aqueous solutions of hemoglobin, myoglobin, and bovine serum albumin: the role of structure and ph.	new measurements on ultrasound absorption of aqueous solutions of the three globular proteins, bovine hemoglobin, horse heart myoglobin, and bovine serum albumin, at 20 degrees c are reported for the frequency range below 1.2 mhz and, in the case of hemoglobin and bovine serum albumin, to a limited extent for the range 2-30 mhz. the effect of protein conformation has been investigated by use of a range of denaturants and by change of ph. a much more detailed description of protein interactions e ...	1988	3411029
a plague epizootic in the white-tailed prairie dogs (cynomys leucurus) of meeteetse, wyoming.	surveillance for sylvatic plague (yersinia pestis) was conducted near meeteetse, wyoming (usa) from 24 may to 14 june 1985. ten species of fleas were collected from white-tailed prairie dogs (cynomys leucurus), and from their burrows and associated rodents. five of these flea species and two adult prairie dogs were positive for plague. the progression of this plague epizootic appeared to be slower and the intensity was less than in previous epizootics in other prairie dog colonies. the plague ep ...	1988	3411698
glutaraldehyde-preserved venous valve transplantation in the dog.	transplantation of femoral vein grafts was performed on 33 mongrel dogs to assess graft patency and valvular function after storage in glutaraldehyde. the grafts were removed from the donor, flushed with room temperature heparinized lactated ringer's solution, and then stored in a 0.2% glutaraldehyde solution for 16 hr. at the time of grafting, the veins were again flushed with lactated ringer's and anastomosed orthotopically to the recipient. an arteriovenous fistula was also created. postopera ...	1988	3411953
relation of changes in amount and type of dietary fat to fecapentaenes in premenopausal women.	correlation studies suggest that fecal mutagenicity is increased in groups eating high-fat diets, the same groups who are often found to have high colorectal cancer incidence and mortality. the fecapentaenes are the best characterized class of fecal mutagens, but the relationship of dietary fat intake to the excretion of these potent genotoxins is unknown. we studied the effect of changes in amount and type of dietary fat on fecapentaene levels in 31 premenopausal women 20-40 years of age who pa ...	1988	3412371
relation of changes in amount and type of dietary fat to fecapentaenes in premenopausal women.	correlation studies suggest that fecal mutagenicity is increased in groups eating high-fat diets, the same groups who are often found to have high colorectal cancer incidence and mortality. the fecapentaenes are the best characterized class of fecal mutagens, but the relationship of dietary fat intake to the excretion of these potent genotoxins is unknown. we studied the effect of changes in amount and type of dietary fat on fecapentaene levels in 31 premenopausal women 20-40 years of age who pa ...	1988	3412371
effects of temporal frequency on the contrast sensitivity of the human accommodation system.	contrast thresholds were determined for the initiation of accommodative responses to drifting sine-wave stimuli (4.1 c/deg) over a range of temporal frequencies (0-14.0 hz). accommodation was monitored with a dynamic infrared optometer. psychophysical detection thresholds were also determined for the same stimuli. a comparison of the contrast thresholds for detection with those for the initiation of accommodation indicates that these two tasks are mediated by mechanisms of comparable sensitivity ...	1988	3414014
experimental contact sensitization with 3,4,5-trichloropyridazine.	the potential of 3,4,5-trichloropyridazine to induce contact sensitization was assessed in the guinea pig maximization test of magnusson and kligman and also in the closed patch test described by buehler. the test material was a 1% solution of 3,4,5-trichloropyridazine in a highly refined mineral oil. the test material elicited moderate to severe irritation when diluted in mineral oil to concentrations of 15-25% and minimal irritation at concentrations of 1-3%. both tests clearly indicated that ...	1988	3416588
genetic characterization of leishmania isolates at 37 enzyme loci.		1988	3417374
optic disc, cup and neuroretinal rim size, configuration and correlations in normal eyes.	four hundred and fifty-seven unselected normal human optic nerve heads of 319 subjects (163 men, 156 women, mean age 42.7 +/- 19.6 years) were evaluated by magnification-corrected morphometry of optic disc photographs. mean optic disc surface measured 2.69 +/- 0.70 mm2 (0.80-5.54 mm2), mean diameter horizontally 1.76 +/- 0.31 mm (0.91-2.61 mm), and vertically 1.92 +/- 0.29 mm (0.96-2.91 mm). the form was slightly vertically oval. optic cup area averaged 0.72 +/- 0.70 (0.00-3.41 mm2), mean horizo ...	1988	3417404
spinal cord penetration of intrathecally administered cytarabine and methotrexate: a quantitative autoradiographic study.	carcinomatous meningitis is an increasingly common complication of systemic cancer. treatment usually involves the use of intrathecal methotrexate or cytarabine, which have been reported to be toxic to the spinal cord. this study was performed to determine the penetration and distribution of intrathecally administered chemotherapy within the spinal cord. tritiated methotrexate and cytarabine were administered intraventricularly and by lumbar puncture to new zealand white rabbits. the extent and ...	1988	3418727
the effects of aspirin-like drugs on the nutritional status of pregnant rats and offspring.	previous studies have shown that salicylates and protein-calorie malnutrition independently compromise maturation and growth of infants. in the present study, pregnant rats were fed normal-and low-protein diets with and without aspirinlike drug treatments. the effects of a low-protein (8 percent) diet included decreases in total serum proteins in both dams and their offspring. decreases in weight gains, serum albumin and globulin levels, and blood urea nitrogen (bun) concentrations were detected ...	1988	3418736
profile of patients from a veterans administration medical center.	data were analyzed to establish a general patient profile that could be the basis for specific profiles to be utilized for infectious disease and microbiologic studies. the analysis revealed that the average patient in this institution is a 57-year-old, white, married, protestant man residing in oklahoma.a personal-interview survey of 42 inpatients showed that 95 percent had smoked for a number of years, and of the smokers, 60 percent were current smokers while the remaining 40 percent had stopp ...	1988	3418737
virus-pesticide interactions with murine cellular immunity after sublethal exposure to dieldrin and aminocarb.	interaction of two potential immunosuppressive factors, sublethal pesticide exposure and viral inhibition of lymphocyte mitogenesis, was examined in mixed lymphocyte reaction (mlr). inbred (c57bl/6 x a/j)f mice, semisusceptible to mouse hepatitis virus 3 (mhv3) infection were exposed to selected pesticides and subsequently infected with the mhv3 virus. the mortality of animals was examined as a function of pesticide exposure. two pesticides were selected for further studies: the organochlorine p ...	1988	3418741
common genetic pools between human populations.	a further inquiry on modern human origins, based on common genetic pool surveys of rigorously selected population samples and highly informative immunological polymorphisms, provides new evidence of an occidental-oriental population split as the origin of human gene pool divergences. the most likely ancestral genetic profile is discussed in the context of the debate raised by preliminary dna restriction fragment length polymorphism studies, which contradict the conclusions drawn from classical b ...	1988	3422217
native genomic blotting: high-resolution mapping of dnase i-hypersensitive sites and protein-dna interactions.	dnase i-hypersensitive sites are observed in the promoter regions of actively expressed genes, potentially active genes, and genes that were once active. we have developed an approach that greatly increases the resolution for mapping these sites by electrophoresing genomic dna on native polyacrylamide gels prior to electroblotting and hybridization. this improved method has been used to scan the promoter and coding region of a cell-cycle-dependent human histone h4 gene with an accuracy of +/-5-1 ...	1988	3422413
[levels of prostaglandin e1 and f2 alpha in the dynamics of toxic-infectious shock induced by yersinia pestis].	murine toxin of yersinia pestis when injected in the rat tail vein (ld50) caused pronounced alterations in pge1 and pgf2 alpha content in different tissues (lung, heart, spleen, liver, kidney, small intestine) and blood. heat-inactivated toxin has been shown to have the same effects as the intact toxin preparation. the changes in pg content are, probably, due to the lipopolysaccharide component of both preparations. the differences in metabolic effects between yersinia pestis endotoxin and lipop ...	1988	3162386
luteolytic potency of 16-phenoxy-derivatives of prostaglandin f2 alpha.	the binding of 16-phenoxy derivatives of prostaglandin (pg) f2 alpha to rat luteal membranes, and also their abortifacient potency in pregnant rats, have been studied. competitive binding studies with various pg-analogues were performed in ovaries of juvenile rats pretreated with pmsg and hcg, and in parallel studies the abortifacient potency of these substances was tested in pregnant rats. it was observed that this class of derivatives bound to the pgf2 alpha receptor as well as, or even better ...	1988	3162421
mechanisms of conversion of xanthine dehydrogenase to xanthine oxidase in ischemic rat liver and kidney.	previous studies have proposed and supported a role for the proteolytic, irreversible conversion of xanthine dehydrogenase to xanthine oxidase (xo) in postischemic injury in a wide variety of organs. a second mechanism of conversion, due to sulfhydryl modification and reversible with dithiothreitol (dtt), is potentially important but has not been well investigated. in this study rat liver and kidney were found to produce significant amounts of dtt-reversible xo during normothermic global ischemi ...	1988	3163235
a double-blind comparison of ibuprofen, placebo and ibuprofen with meptazinol in soft tissue rheumatism.	the administration of ibuprofen potentiates and prolongs the analgesic effect of meptazinol when the two drugs are given simultaneously to mice. a double-blind three-way crossover study of placebo, ibuprofen (1600 mg/day) and ibuprofen (1600 mg/day) plus meptazinol (400 mg/day) was carried out in 45 patients with soft tissue rheumatism to see if the same potentiation could be demonstrated in man. treatment order was randomized and each regimen was given for 2 weeks preceded by 1 week on paraceta ...	1988	3163538
jamshidi needle biopsy for diagnosis of bone lesions in small animals.	sixty-two bone lesions in 59 small animals were biopsied using a jamshidi-type biopsy needle. in all instances, the jamshidi needle biopsy diagnosis was confirmed or disputed by results of histologic examination of specimens obtained by surgical resection or by amputation or at necropsy. in 57 of 62 bone lesions, biopsy resulted in an accurate diagnosis of tumor vs nontumor, yielding an accuracy rate of 91.9%. the specific tumor type or lesion type was identified accurately by jamshidi needle bi ...	1988	3165373
[an immunocytochemical study on the prostaglandin e2 localization in human gastric mucosa].		1988	3165480
production of tumor necrosis factor in nude mice by muramyl peptides associated with bacterial vaccines.	gram-negative vaccines can elicit the production of tumor necrosis factor (tnf) in mice primed by muramyl dipeptide (mdp) or by its lipophilic derivative mdp-dipalmitoyl glycerol (mdp-gdp). in mice pretreated with mdp and particularly with mdp-gdp, bordetella pertussis vaccine was shown to be more effective than typhoid vaccine. the time course of tnf production in the blood did not indicate any difference between the effect of mdp or of mdp-gdp. in both cases the cytotoxic activity reached maxi ...	1988	3167834
a new approach to the quality control of steroid receptor assays.	lyophilized cytosols prepared from calf uterus and human breast tumor tissue are commonly used to assess the reliability of routine steroid receptor assays. however, preanalytical error (sample preparation, storage, homogenization) cannot be detected in this way. participating laboratories were asked to mail us all their receptor results obtained over a four-month interval, and to include some information about the patients involved (age, menopausal status, nodal status). after verifying the hom ...	1988	3168219
[effect of the entomopathogenic fungus penicillium funiculosum on the reproduction of the causative agent of plague in the body of fleas].		1988	3173243
[qualitative and quantitative changes in the phospholipid composition in experimental brain edema and during treatment with antiedema preparations].		1988	3174245
receptor-like function of heparin in the binding and uptake of neutral lipids.	molecular mechanisms regulating the binding, amphipathic stabilization, and metabolism of the major neutral lipids (e.g., cholesteryl esters, triglycerides, and fatty acids) are well studied, but the details of their movement from a binding compartment to a metabolic compartment deserve further attention. since all neutral lipids must cross hydrophilic segments of plasma membranes during such movement, we postulate that a critical receptor-like site exists on the plasma membrane to mediate a ste ...	1988	3174644
funding of project to map human genes sparks debate.		1988	3177203
phasic activity of human dorsal foot veins.	phasic contractions of the dorsal foot veins were detected in 7 male subjects, aged 23 to 49 years, by means of a linear variable differential transformer (lvdt) after congesting the veins by a pressure of 6.7 kpa (50 torr). this phasic activity (pa) had a frequency of 3 to 7 contractions per minute and led to a periodic diminution of the diameter of the distended veins by 12 to 44%. using a double lvdt, the pa was shown to represent peristaltic waves travelling along the vessel wall concomitant ...	1988	3178793
[identification of the principal species of the genus serratia by a simplified system].	a reduced identification system for the genus serratia is proposed. the usefulness of this system was shown by identification of 720 strains from clinical specimens or natural environment. computation of "diagnosis ability coefficient" and principal component analysis made it possible to reduce this identification system to 9 tests. numerical taxonomy displayed the 9 recognized species.	1988	3179060
localization of the human dopamine beta hydroxylase (dbh) gene to chromosome 9q34.	a human cdna clone for dopamine beta hydroxylase (dbh) has been isolated from a phaeochromocytoma library. in situ hybridization of this probe to replication-banded chromosomes has localized the gene to chromosome 9q34. the structural gene for the enzyme is therefore close to the abo blood group locus. this suggests that the previously described activity variation in levels of serum dbh may reflect alterations in either the structure or regulation of the dbh coding sequences. both biochemical an ...	1988	3180847
some chemical properties of maitotoxin, a putative calcium channel agonist isolated from a marine dinoflagellate.	maitotoxion, a putative ca2+ channel agonist, was isolated from cultures of the marine dinoflagellate gambierdiscus toxicus as a colorless amorphous solid. the toxin reacted positively to dragendorff's reagent but not to ninhydrin reagent. the mouse lethality of maitotoxin determined by intraperitoneal injection was 0.13 micrograms/kg. chemical features of the toxin were elucidated mainly by various spectroscopic measurements. the molecular weight of maitotoxin as a disodium salt was estimated t ...	1988	3182760
some chemical properties of maitotoxin, a putative calcium channel agonist isolated from a marine dinoflagellate.	maitotoxion, a putative ca2+ channel agonist, was isolated from cultures of the marine dinoflagellate gambierdiscus toxicus as a colorless amorphous solid. the toxin reacted positively to dragendorff's reagent but not to ninhydrin reagent. the mouse lethality of maitotoxin determined by intraperitoneal injection was 0.13 micrograms/kg. chemical features of the toxin were elucidated mainly by various spectroscopic measurements. the molecular weight of maitotoxin as a disodium salt was estimated t ...	1988	3182760
a role for glyceraldehyde-3-phosphate dehydrogenase in the development of thermotolerance in xenopus laevis embryos.	during heat shock, xenopus laevis embryos exhibit an increase in the rate of accumulation of lactate and a loss of atp relative to non-heat-shocked control embryos. these results suggest that heat shock stimulates a shift in energy metabolism to anaerobic glycolysis while at the same time causing an increase in the demand for atp. we have evidence indicating that the embryo may meet such demands placed on it by increasing the levels of some glycolytic enzymes. in this report, we show that heat s ...	1988	3182940
organization of a complex movement: fixed and variable components of the cockroach escape behavior.	the escape behavior of the cockroach periplaneta americana was studied by means of high speed filming (250 frames/s) and a computer-graphical analysis of the body and leg movements. the results are as follows: 1. the behavior begins with pure rotation of the body about the posteriorly located cerci, followed by rotation plus forward translation, and finally pure translation (figs. 1, 2). 2. a consistent inter-leg coordination is used for the entire duration of the turn (fig. 3a). at the start of ...	1988	3184004
[effect of the induction of liver cytochrome p-450 by a chemically inert fluorocarbon on the reactions of immediate and delayed hypersensitivity].	the effect of chemically inert perfluorodecalin (pfd) on the development of hypersensitive reactions of immediate and delayed types in guinea pigs and mice was studied. it was found that administration of pfd to animals both before sensitization (mice) and during the development of sensitization (guinea pigs) reduced the degree of the both types of hypersensitivity.	1988	3191983
effect of bromoethylamine hydrobromide on systemic acid-base balance.	intravenous administration of bromoethylamine hydrobromide (bea) has been shown to induce papillary necrosis of the kidney. we used this model to clarify the role of the medullary structure in acid-base homeostasis. bea (25 mg) or vehicle was injected to male sprague-dawley rats. blood specimens and 24 hr urine were collected once a week totaling 4 weeks. blood bicarbonate significantly decreased in bea treated rats with no change in plasma creatinine or creatinine clearance at 3 and 4 weeks, we ...	1988	3194903
treatment of fasciola hepatica infection in horses with triclabendazole.	thirteen thoroughbred foals with an adult infection and a presumed immature infection with fasciola hepatica were treated with 12 mg triclabendazole/kg bodyweight. the absence of eggs from samples of faeces examined at intervals up to 110 days after treatment showed that all the animals were cured. in a second trial the same dose of triclabendazole cured 11 of 12 foals whereas nitroxynil at 7 mg/kg cured only six of 12 foals. no side effects were observed after treatment.	1988	3195012
mutations in the hemagglutinin gene associated with influenza virus resistance to norakin.	the nucleotide sequences of the hemagglutinin genes of four norakin-resistant mutants of influenza a/fpv/weybridge were determined and compared to the wild-type hemagglutinin. all mutants show one or two amino acid substitutions which are discussed to destabilise the ph 7 conformation of hemagglutinin.	1988	3196166
seronegative virus carriers in the infection of rabbits with human t lymphotropic virus type i.	six htlv-i-transformed t cell lines were prepared from pbl of three rabbits each of b/j and chbb:hm strains, and were inoculated into newborn rabbits of these two strains, and of their f1 hybrid. none of three b/j cell lines induced anti-htlv-i antibody response in newborn b/j rabbits, whereas all three chbb:hm cell lines did induce a response in newborn chbb:hm rabbits. these b/j cell lines however could induce antibody response in adult b/j as well as newborn chbb:hm rabbits, and a chbb:hm cel ...	1988	3199075
seronegative virus carriers in the infection of rabbits with human t lymphotropic virus type i.	six htlv-i-transformed t cell lines were prepared from pbl of three rabbits each of b/j and chbb:hm strains, and were inoculated into newborn rabbits of these two strains, and of their f1 hybrid. none of three b/j cell lines induced anti-htlv-i antibody response in newborn b/j rabbits, whereas all three chbb:hm cell lines did induce a response in newborn chbb:hm rabbits. these b/j cell lines however could induce antibody response in adult b/j as well as newborn chbb:hm rabbits, and a chbb:hm cel ...	1988	3199075
complex organization of the length heterogeneous 5' external spacer of mung bean (vigna radiata) ribosomal dna.	restriction enzyme analysis and cloning of the 18s, 5.8s, and 25s ribosomal rna genes (rdna) of the mung bean (vigna radiata = phaseolus aureus) reveal length heterogeneity in the repeating units (10-11 kbp) localized within two different regions in the ribosomal spacer. the 1.5-2.0 kbp region flanking the 3' end of the 25s rrna contains various numbers (8-10) of tandemly arranged 180 bp subrepeats. after dna sequencing a complex organized length heterogeneous 5' external spacer built up of diff ...	1988	3203889
double myelination of axons in the sympathetic nervous system of the mouse. ii. mechanisms of formation.	the phenomenon termed 'double myelination', present in sympathetic nerve of normal adult rats and mice, comprises regions of a myelinated axon which are concentrically ensheathed by additional (outer) myelinating schwann cells. evidence has been presented that in some instances the outer schwann cell fails to make contact with an axon, yet its myelin sheath characteristically remains ultrastructurally intact. the present study has sought to identify and analyse configurations intermediate betwee ...	1988	3204414
diseases of winter housed sheep.		1988	3206810
[changes in the "latent" virulence of a vaccinal strain of yersinia pestis multiplying within macrophages].	the multiplication of y. pestis vaccinal strain inside peritoneal macrophages of guinea pigs and white mice in vitro leads to an essential increase in its latent virulence. this effect is most pronounced when guinea pig macrophages are used. changes in the latent virulence of y. pestis vaccinal strains, occurring in the process of their passage inside macrophages in vitro, correlate with those observed in vivo, i.e. in animal experiments.	1988	3206961
[bactericidal effect of tween 80 on mycobacteria].		1988	3210511
effect of aminooxyacetic acid on the release of preloaded [3h]gaba and radioactive metabolites from slices of developing mouse brain.	the release of [3h]gamma-aminobutyric acid (gaba) and its radioactive metabolites from slices of the cerebral cortex, cerebellum, striatum and brain stem of developing and adult mice was studied. the slices were incubated and superfused in the absence and presence of the gaba aminotransferase (gaba-t) inhibitor aminooxyacetic acid (aoaa). exposure to 100 microm aoaa totally inhibited gaba-t and all radioactivity released from slices was in authentic gaba. in studies on developing brain the 10-mi ...	1988	3216949
[prospects for the use of monoclonal antibodies in yersinia pestis].	industrial production of plague monoclonal antibodies in mice balb/c was shown possible. precipitation of the immunoglobulins with caprylic acid provided the highest purity of the preparations. maintenance of the specific activity by the monoclonal antibodies was stable. the immunoenzymatic conjugate and erythrocytic diagnosticum on the basis of the monoclonal antibodies are sensitive, specific and may be used in antiplague practice.	1988	3218993
morphological variation of echinococcus granulosus protoscoleces from hydatid cysts of human and various domestic animals in jordan.		1988	3220653
observations on commensal rats and their status to plague in bombay.	the distribution pattern of various types of commensal rodents in bombay city reveals that bandicota bengalensis constitutes the predominant commensal rodent species followed by r. rattus and rattus norvegicus. apart from these three types, bandicota indica, m. musculus and an insectivore (suncus murinus) are the three species of commensal small mammals that are frequently encountered in or near human habitations. these small mammals are prevalent throughout the year and their percentage distrib ...	1988	3221091
[behavioral, morphologic and biochemical correlates of the early effect of a neural transplant into the damaged brain of adult rats].	complex study was carried out of the behavioural, morphological and neurobiochemical parameters of the effect of an embryonal neural transplant of the amygdala in the first weeks after its introduction into the area of the left amygdala of adult rats-recipients. the neural transplant promoted normalization of animals food search, disturbed by operative interference. the amygdalar embryonal tissue lowered the proliferative glia reaction, produced by the brain lesion, and intensified the nervous t ...	1988	3223076
beneficial effect of omeprazole in a patient with severe bronchial asthma and gastro-oesophageal reflux.	a 25-yr-old man suffered from severe nocturnal asthma, which was shown to be provoked by pathological gastro-oesophageal reflux. a dramatic, immediate improvement of his pulmonary condition was achieved by treatment with omeprazole after failure of other therapeutic measures, including high doses of ranitidine.	1988	3224694
identification of a yersinia pestis-specific dna probe with potential for use in plague surveillance.	a 900-base-pair dna fragment derived from a 9.5-kilobase plasmid in yersinia pestis hybridized specifically with y. pestis dna. we demonstrated the feasibility of using this dna fragment to detect plague bacilli directly in fleas, suggesting that this y. pestis-specific dna probe may be used for plague surveillance in the field. additional applications for this dna probe may include plague diagnosis and pathogenesis research.	1988	3230131
role of renal prostaglandins in bile-induced diuresis in the dog.	to clarify the possible role of renal prostaglandins (pgs) in the phenomenon of bile-induced diuresis in the dog, we studied the effect of in situ unilateral infusion of bile on kidney function and pgs excretion, before and after pg-synthetase inhibitor administration in anesthetized dogs. the contralateral intact kidney served as control. in the first group of 6 dogs, infusion of bile diluted 1:20 resulted in a significant increase in urinary flow (117%; p less than 0.05), sodium (61%; p less t ...	1988	3231185
importance of a 3-o-sulfate group in a heparin pentasaccharide for antithrombotic activity.	previous theoretical and experimental evidence led to the formulation of a specific pentasaccharide structure which represents the site in heparin for binding to antithrombin iii. this pentasaccharide was subsequently synthesized. a pentasaccharide of the same structure but lacking only the sulfate group on the hydroxyl group of the middle glucosamine (position c-3) was also synthesized to test the structure - activity relationships. previous biochemical studies showed the 3-o-desulfated pentasa ...	1988	3232125
examining the impact of different health levels and management strategies on dairy heifer production through computer simulation.		1988	3232667
magnesium salts exert direct vasodilator effects on rat cremaster muscle microcirculation.	in vivo microscopic observations were made on arterioles and venules (10-20 microns i.d.) of the rat cremaster muscle circulation, using an image-splitting eyepiece and high-resolution tv image-intensification, in order to determine the microvascular effects of inorganic and organic magnesium (mg) salts. mgso4, mgcl2, mg aspartate hcl, and mg acetate were administered perivascularly and intravenously. topical application (10(-2) to 10(1) mumol) and intravenous (i.v.) administration (5-400 mumol/ ...	1988	3233048
effects of acute doses of ethanol administered at pre-implantation stages on fetal development in the mouse.	the objective of the present study was to investigate the effects of maternal administration of acute doses of alcohol at preimplantation stages of pregnancy on the fetus and fetal membranes. single doses of 0.02 ml and 0.03 ml/g body wt. of 25% v/v of absolute alcohol in saline were administered to groups of mf1 mice on one of days 1-6 of gestation. controls were either pair-fed and pair-watered or saline-treated. fetuses and placentae were collected on day 15. resorption and fetal death were i ...	1988	3234238
periodicity coding in the inferior colliculus of the cat. ii. topographical organization.	1. the topographical distributions of single-unit and multiple-unit responses to amplitude-modulated tones--and to other relevant parameters of simple tonal stimuli--were defined across the main frequency representational gradient and within narrow frequency ranges represented in "frequency band laminae" in the principal midbrain auditory nucleus, the central nucleus of the inferior colliculus (icc), in adult, barbiturate-anesthetized cats. 2. responses to amplitude-modulated tones with the carr ...	1988	3236053
periodicity coding in the inferior colliculus of the cat. ii. topographical organization.	1. the topographical distributions of single-unit and multiple-unit responses to amplitude-modulated tones--and to other relevant parameters of simple tonal stimuli--were defined across the main frequency representational gradient and within narrow frequency ranges represented in "frequency band laminae" in the principal midbrain auditory nucleus, the central nucleus of the inferior colliculus (icc), in adult, barbiturate-anesthetized cats. 2. responses to amplitude-modulated tones with the carr ...	1988	3236053
dimetridazole residues in pork tissue. i. assay by liquid chromatography with electrochemical detector.	a liquid chromatographic (lc) method with electrochemical detection in the reductive mode was developed for the quantitative determination of dimetridazole (dmz) and its major metabolite (hmmni) at residue levels in pork tissue. for blood plasma, a sample is precipitated with 2 volumes of acetonitrile and centrifuged, and a diluted aliquot of the supernatant liquid is chromatographed. for muscle, a 10 g sample is extracted 3 times with dichloromethane. after evaporation of the combined extracts, ...	1988	3240970
the effect of methylxanthines on morphine analgesia in mice and rats.	the influence of various doses of caffeine and theophylline on analgesic effect of morphine was tested in mice and rats in the hot plate and tail immersion tests. caffeine in rats potentiated the morphine analgesia in both tests in a dose-dependent manner. in mice the caffeine-morphine interaction was biphasic: lower caffeine doses (10 and 25 mg/kg) inhibited, while higher (75 and 100 mg/kg) potentiated the analgesic effects of morphine. theophylline interacted with morphine in a manner similar ...	1988	3241763
[study of the virulence plasmids of 100 strains of yersinia pestis isolated in madagascar].		1988	3242429
[9,12,13-trihydroxy-10(e)-octadecenoic and 9,12,13-trihydroxy-10,11-epoxy-octadecanoic acids--new antistress substances isolated from licorice].		1988	3243940
purification, partial characterization and biological effects of the xtc mesoderm-inducing factor.	the mesoderm of xenopus laevis is formed through an inductive interaction in which a signal from the vegetal hemisphere of the blastula acts on overlying animal pole cells. we have recently reported that the xenopus xtc cell line secretes a mesoderm-inducing factor (mif) which may resemble the natural signal. in this paper, we describe the purification and biological effects of xtc-mif. xtc-mif is a hydrophobic protein with an isoelectric point of 7.8 and an apparent relative molecular mass (mr) ...	1988	3246228
purification, partial characterization and biological effects of the xtc mesoderm-inducing factor.	the mesoderm of xenopus laevis is formed through an inductive interaction in which a signal from the vegetal hemisphere of the blastula acts on overlying animal pole cells. we have recently reported that the xenopus xtc cell line secretes a mesoderm-inducing factor (mif) which may resemble the natural signal. in this paper, we describe the purification and biological effects of xtc-mif. xtc-mif is a hydrophobic protein with an isoelectric point of 7.8 and an apparent relative molecular mass (mr) ...	1988	3246228
[studies on the nontuberculous lung mycobacteriosis in japan (report of the study in the year 1986 of the mycobacteriosis research group of the japanese national chest hospitals)--the prevalence rate of nontuberculous lung mycobacteriosis in japan is gradually increasing since 1984--the mycobacteriosis research group of the japanese national chest hospitals].		1988	3249525
[studies on the nontuberculous lung mycobacteriosis in japan (report of the study in the year 1986 of the mycobacteriosis research group of the japanese national chest hospitals)--the prevalence rate of nontuberculous lung mycobacteriosis in japan is gradually increasing since 1984--the mycobacteriosis research group of the japanese national chest hospitals].		1988	3249525
[changes in the biological properties of yersinia pestis during long-term intracellular multiplication].		1988	3249571
[the modifying action of normal human blood serum on vaccinal ev76 and virulent 363(1/1479) strains of yersinia pestis].		1988	3249590
[studies on stimulation of immunological responses resulting from intravesical bcg instillation in patients with bladder carcinomas].		1988	3251104
influenza-like syndrome in homosexual men: a prospective diagnostic study.	in the course of a prospective study of the prevalence and incidence of infection with the human immunodeficiency virus (hiv) and risk factors for the acquired immune deficiency syndrome among 961 homosexual men, 97 initially hiv antibody seronegative men reported a febrile period lasting at least three days. in 60 of these men serological evidence for an infection was found: influenza a or b virus (17 men), hiv (14), epstein-barr virus (seven), parainfluenza virus type i, 11 or ill (five), hepa ...	1988	3256668
glutathione transferase activities of cultured human lymphocytes.	we have detected glutathione transferase (gst) activity towards 1-chloro-2,4-dinitrobenzene (cdnb) and benzo[a]-pyrene 4,5-oxide (bpo) in freshly isolated peripheral lymphocytes, phytohaemagglutinin (pha)-stimulated lymphocytes, interleukin-2 (il-2)-dependent t-cells and lymphoblastoid b-cell lines. the detection of conjugating activity with bpo implies the expression of a neutral, 'mu-like' gst form by both resting and cultured lymphocytes. in a sample of 12 unrelated individuals, bpo conjugati ...	1988	3257909
inhibition of the proliferative response of human b lymphocytes to b cell growth factor by transforming growth factor-beta.	the effects of transforming growth factor-beta (tgf-beta) on the proliferative response of human b cells to the low molecular weight b cell growth factor (bcgf) have been investigated in this study. it was found that tgf-beta, at picomolar concentrations, strongly inhibited the bcgf-induced proliferation of anti-mu chain or staphylococcus aureus cowan i-activated human b cells and also of a bcgf-dependent cell line derived from a human lymphocytic nodular lymphoma. this inhibitory effect was det ...	1988	3257917
nonrandom trg gamma variable gene rearrangement in normal human t cells and t cell leukemias.	to estimate the extent of the trg gamma variable (v) gene repertoire used in human t cell ontogeny, we have analyzed the variety of v gamma gene rearrangements in a large series of t and non-t acute and chronic leukemias. a limited heterogeneity of rearranged fragments was observed: only 13 types of differently rearranged fragments, four of which occurred only once, were found among 80 rearranged chromosomes. furthermore, in the leukemic population as a whole, the frequency distribution of the m ...	1988	3257920
a monoclonal antibody for the specific diagnosis of plague.	a stable mouse-cell hybridoma was obtained that secretes an iga monoclonal antibody reactive with the fraction 1 (f1) envelope antigen of yersinia pestis. titres of the antibody typically ranged from 1:32 768 to 1:65 536 in mouse ascitic fluids.the monoclonal antibody formed a line of precipitation when run against f1 antigen in ouchterlony gel diffusion tests. in tests of 235 strains of y. pestis, lines of identity occurred between the precipitates formed with a solution of purified f1 antigen ...	1988	3260145
[a genetically defined model for testing teratogenicity in vivo].		1988	3261883
potent and comparable vasodilator actions of a- and b-calcitonin-gene-related peptides on the superficial subcutaneous vasculature of man.	1. vasoactivity of human a- and b-calcitonin-gene-related peptides was studied in normal subjects, using reflectance plethysmography to measure relative changes in blood flow produced by superficial subcutaneous injections of the peptides. 2. injection of 10(-11) mol of either peptide caused an immediate 200% increase in local blood flow and prolonged hyperaemia lasting up to 3 h. the hyperaemic response to 10(-13) mol of each peptide was significantly (p less than 0.01) smaller and shorter in d ...	1988	3262028
receptor-mediated endocytosis and degradation of bovine growth hormone in rat liver.	receptor-mediated endocytosis of radiolabelled bovine growth hormone (125i-bgh) via somatogenic receptors in the liver was studied following in vivo intraportal injection. at different times after injection, subcellular membrane fractions involved in binding (plasma membranes), endocytosis (endocytic vesicles) and degradation (lysosomes) of peptide hormones were isolated by sucrose density gradient centrifugation. these fractions were evaluated for the time-course accumulation of radiolabelled b ...	1988	3264255
interleukin 1 as a tumor cytostatic mediator released from tumor ascites-treated macrophages.	peritoneal macrophages from dba/2 mice, elicited by injection of corynebacterium parvum (c.p.), were in vitro activated to eb tumor cytostasis by incubation with tumor-induced ascites that was harvested 7 days after intraperitoneal eb injection. the active cytostasis-mediating compound was found to be interleukin 1 (il 1). when tumor ascites was fractionated according to molecular weight size, the most active il 1-inducing fraction was found to comprise molecules of greater than 100,000 daltons. ...	1988	3264264
t-cell subsets in the cerebrospinal fluid and blood of patients with multiple sclerosis.	the absolute numbers and ratios of helper/inducer (t4) and cytotoxic/suppressor (t8) t-cells were determined in cerebrospinal fluid (csf) and blood of patients with multiple sclerosis (ms) and various other neurologic diseases (ond). in patients with ms, the t4:t8 ratio was higher in both blood and csf, and the increase was significantly greater in csf than in blood. these findings were due to an increased proportion of t4-lymphocytes in the csf and to a decreased proportion of t8-cells in blood ...	1988	3264287
suppressive and enhancing effect of t-2 toxin on murine lymphocyte activation and interleukin 2 production.	t-2 toxin, a fungal metabolite shown previously to exert potent immunosuppressive effects, was examined for its effects on activation and interleukin 2 (il 2) production by murine and rat splenocytes. splenocytes (1 x 10(6) cells/well) were incubated with 1 microgram concanavalin a (con a) for 48 h at which time cellular protein and dna synthesis by these cells were ascertained using radiolabeled precursors. il 2 synthesis was determined from the cell supernatant using the il 2-requiring cell li ...	1988	3264300
malaria infection in rats with stimulated splenic red pulp: the blood flow and protective effect in normal and transplanted splenic tissue.	after splenic autotransplantation both weight and blood flow of the regenerated splenic tissue are decreased. in addition, the protective function of the transplant is less compared to that of the normal spleen. in the present study, the red pulp of normal and transplanted splenic tissue was stimulated by injections of phenylhydrazine to increase the weight, the blood flow, and the protective function. after stimulation, the weight of the normal spleen increased to 900 +/- 70 mg (control 530 +/- ...	1988	3265528
optimal conditions for recovery of the human immunodeficiency virus from peripheral blood mononuclear cells.	optimal conditions for demonstrating the presence of infectious human immunodeficiency virus in peripheral blood mononuclear cells (pmcs) from seropositive individuals involved cocultivation of infected cells with phytohemagglutinin-stimulated pmcs from seronegative donors in the presence of 2 micrograms of polybrene per ml. the size of the culture vessel also influenced the results; smaller numbers of infected cells were detected under conditions of increased cell density. in addition, an incre ...	1988	3266220
circular intermediates with missing nucleotides in the conversion of supercoiled or nicked circular to linear duplex dna catalyzed by two species of bal 31 nuclease.	the extracellular nucleases from alteromonas espejiana bal 31 can catalyze the endonucleolytic and/or exonucleolytic hydrolysis of duplex dna in response to a variety of alterations, either covalent or noncovalent, in dna structure. the nuclease can exist as at least two kinetically and molecularly distinct protein species. the two species that have been studied, called the 'fast' (f) and 'slow' (s) nucleases, both readily convert negatively supercoiled dnas to linear duplex molecules and accomp ...	1988	3275464
plasmodium fragile: detection of a ring-infected erythrocyte surface antigen (resa).	a ring-infected erythrocyte surface antigen (resa) has been detected by modified immunofluorescence assay in erythrocytes infected with the simian malaria parasite, plasmodium fragile. this resa, of mr 95,000, shares many characteristics with the resa initially found in the human malaria parasite p. falciparum. both antigens are found in the membrane of erythrocytes infected with young asexual parasite stages, in merozoite-enriched preparations, and in parasite culture supernatant. since the res ...	1988	3276549
the effects of some synthetic prostanoids on the contractility of the human lower uterine segment in vitro.	we have investigated the ability of three synthetic prostanoids to directly influence uterine contractility by studying the effects in vitro. strips of lower uterine segment smooth muscle were obtained from women undergoing elective cesarean section at term. the ability of these strips to develop tension in the presence of cumulative additions of prostanoids or oxytocin was assessed. spontaneous contractions were inhibited by zk 96.480, a stable synthetic analog of prostaglandin i2, with a 50th ...	1988	3277428
[continuous nasal positive pressure respiration (ncpap) as a therapeutic possibility in the sleep apnea syndrome].	sleep apnea syndrome (sas) is a rare disorder which is being diagnosed more often with increasing knowledge among physicians and patients. sas presents with daytime hypersomnolence, intellectual deterioration and personality changes, chiefly in obese men, and is caused by intermittent upper airway obstruction during sleep at the level of the mesopharynx. consecutive repetitive apneas of more than 10 seconds' duration are immediately abolished by pneumatic splinting with continuous positive press ...	1988	3278374
a comparison of culture, direct fluorescent antibody test, and a quantitative indirect immunoperoxidase assay for detection of chlamydia trachomatis in pregnant women.	an indirect immunoperoxidase assay that quantitates antichlamydial immunoglobulin g (igg) and iga antibodies in serum was compared with endocervical culture and direct fluorescent antibody (microtrak) for detection of chlamydia trachomatis in asymptomatic pregnant women. of the 64 women tested by the three methods, 22 (34%) had antichlamydial igg and iga in their serum. the culture was positive in nine patients (14%) and the microtrak was positive in eight (12.5%). all positive cultures were imm ...	1988	3279354
vitamin d binding protein (gc) subtypes by isoelectric focusing and immunoblotting.	the polymorphism of the human vitamin d binding protein (gc system) was investigated in a total of 149 sera from unrelated healthy egyptians residing in tanta city, gharbiya governorate, nile delta of egypt, using isoelectric focusing (ief) in thin-layer polyacrylamide gel followed by immunoblotting. the estimated gene frequencies were gc1s = 0.540, gc1f = 0.242 and gc2 = 0.218.	1988	3280448
biotyping of aerobic actinomycetes by modified killer system.	forty-four yeasts belonging to the genera pichia, candida, saccharomyces and kluyveromyces were tested for their potential killer effect on 13 aerobic actinomycetes (6 nocardia asteroides, 1 n. brasiliensis, 1 n. caviae and 5 actinomadura madurae). only a few yeast strains did not display any killer activity against the aerobic actinomycetes studied, thus confirming that the killer phenomenon is widespread among microorganisms. for epidemiological purposes, a killer system was developed. accordi ...	1988	3281858
[interrelation between the blood neutrophil damage test and the passive hemagglutination reaction in detecting antibodies].	statistical analysis of the results of examinations of vaccinees against plague has revealed that the values of the neutrophil damage index (ndi) and antibody titers as determined in the passive hemagglutination (pha) test qualitatively (but not quantitatively) correlate. the statistical series for the pha test and ndi belong to different variations, i. e. they describe different functions of antibodies with respect to the same antigen. besides, the determination of ndi permits the detection of ...	1988	3284255
the plague pandemics and the discovery of the plague bacillus.		1988	3289591
comparative biology of test species.	this paper assesses the capacity of animal models to predict human response to carcinogenic agents with consideration for the heterogeneity of humans. it is widely accepted that human susceptibility to toxic substances, including carcinogens, is highly variable. conventional rodent models are usually highly inbred and valued for their ability to display characteristic homogeneity. current practice assumes that the homogeneity of response to toxic agents, including carcinogens, in the rodent mode ...	1988	3289908
application of recombinant dna techniques in the development of viral vaccines.	the wide repertoire of methods developed for the construction and manipulation of recombinant dna molecules enable viral genomes to be cloned and their genes expressed, in whole or in part, in microbial or animal cells in culture. this provides a ready source of many viral components that are otherwise difficult to obtain and in some cases were previously unknown. although immediate applications for vaccine development have concentrated principally upon envelope proteins, the immunological impor ...	1988	3291454
sex difference in prolactin and growth hormone cells in mouse adenohypophysis: stereological, morphometric, and immunohistochemical studies by light and electron microscopy.	prl and gh cells in the adenohypophysis of adult mice were studied with immunohistochemistry and stereological morphometry by light and electron microscopy. we compared findings for males and females. in female mice, most prl-immunoreactive cells (43% of all parenchymal cells) and most gh-immunoreactive cells (33% of all parenchymal cells) were classical prl cells (type i prl cells) or gh cells (type i gh cells), respectively. besides these classical types of cells, we found prl- and gh-immunore ...	1988	3293989
deferoxamine inhibition of malaria is independent of host iron status.	the mechanism whereby deferoxamine (df) inhibits the growth of malaria parasites was studied in rats infected with plasmodium berghei. peak parasitemia was 32.6% (day 14) in untreated controls and 0.15% (day 7) in rats receiving 0.33 mg/g in 8 hourly df injections, subcutaneously. df inhibition of parasite growth was achieved without any reduction in transferrin saturation or hemoglobin synthesis and with only a partial (56%) depletion of hepatic iron stores. dietary iron depletion resulted in a ...	1988	3294334