Publications

properties and regulation of c-1-fructose-1,6-diphosphatase from spinach chloroplasts.	chloroplast fructose diphosphatase (ec 3.1.3.11) was purified according to the procedures of racker and schroeder [1] and buchanan et al. [2] and the properties compared. neither preparation contained fructose diphosphatase from the cytoplasm. the preparations had similar molecular weights, ph optima, affinites for fructose diphosphate and mg-2+ and were similarly activated by edta, dithiothreitol and cystamine. mg-2+, fructose diphosphate and dithiothreitol all activate chloroplast fructose dip ...	1985	238625
program-level evaluation using management information.	this paper has summarized the development of a drug abuse treatment program mis, and has shown that one can initially start with a manual system, use it to advantage, and then move into computer programming. in larger programs, it will most likely be necessary to use the computerized system. however, the author has found that implementation costs have not been great, and the system has certainly been worthwhile in light of the dimensions that have been added to the program. the computer mis that ...	1985	262074
[subcutaneous aspergillosis simulating erythema nodosum].		1985	261930
[what is spri? (sjukvårdens planerings- och rationaliseringsinstitut) (nursing care planning and rationalization institute)].		1985	261486
[effects of different postures on left ventricular function during various trimesters of pregnancy].		1985	261924
[prognostic indices of acute lymphoblastic leukemia in the child. proposal for a prognostic classification and case contribution].		1985	271763
resuscitation: a matter of life and death.		1986	260180
sarcoidosis: it strikes at many organs.		1986	260174
is this the face?		1986	260112
[benign vascular tumor of the stomach].		1986	176499
phase ii trial of 7-day continuous 5-fluorouracil infusion in the treatment of advanced colorectal carcinoma.	thirty-eight patients with advanced colorectal adenocarcinoma were entered on a phase ii trial of 5-fluorouracil (5-fu) in continuous infusion, using a portable pump. half of the patients had been pretreated (n = 19) and 16 of them had received intravenous bolus 5-fu alone or in combination. at the first cycle patients received continuous intravenous 5-fu at the dose of 650 mg/m2 per day for 7 consecutive days. doses were escalated during the following cycles and adjusted according to the toxici ...	1986	1542491
the aberdeen formula: revision of nursing workload per patient as a basis for staffing.		1986	260083
potassium pooling in non-rigid parenteral fluid containers.		1986	260935
evaluation of the effectiveness of ammonium bifluoride treatment in the cleaning of parenteral glassware by an automatic particle counting technique.		1986	260934
immunity: autoimmunity, isoimmunity, and immunodeficiency in the foal.		1986	260999
[immunologic studies in polymyositis syndromes].	sixteen cases of polymyositis were submitted to study. out of these sixteen, nine showed no clinical or paraclinical evidences of cancer, and seven presented an associated cancer. in the first group, a normal immune response was observed, whereas in the second group this response was frankly depressed. emphasis is laid on the significance of the immune response, not only in the diagnosis but as a fundamental factor in prognosis and treatment as well. an early treatment of polymyositis without ca ...	1986	262355
selecting staff for hospice work.		1986	260082
nursing care study: industrial burns.		1986	260075
[clinicopathological analysis of fatal cerebellar hematomas].	an analysis is made of the clinical observations and pathological findings in 16 fatal cerebellar hematomas admitted at the instituto de neurología in montevideo, over a period of 23 years. the absence of definitive lesions of the brainstem in the majority of cases, in spite of the rich symptomatology of brainstem disfunction (horizontal gaze palsies, vegetative syndrome decerebrate movements) is stressed, as well as the rapid evolution of those cases with intraventricular haemorrhage. missed cl ...	1986	262357
molecular alteration of the 140-megadalton plasmid associated with loss of virulence and congo red binding activity in shigella flexneri.	a plasmid of about 140 megadaltons has been associated with the invasiveness of shigella flexneri. upon subculturing in liquid media of fully virulent isolates of shigella flexneri 2a ysh6000, which contains only a 230-kilobase-pair (kbp) plasmid in addition to 3.3- and 4.2-kbp cryptic plasmids characteristic to all s. flexneri strains, loss of invasiveness, loss of congo red binding activity (pcr), and complete loss of, or a deletion, or even a single-site is insertion in the plasmid occurred s ...	1986	3002985
[beef-extract agar with sodium nucleinate as a medium for storage and stabilization of biological properties of shigella].		1986	3078175
repression of adenovirus early gene expression by coinfection with a temperature-sensitive mutant in the immediate-early gene of pseudorabies virus.	wild-type adenovirus was coinfected with a mutant temperature sensitive for the immediate-early gene of pseudorabies virus. at the nonpermissive temperature, this mutant, tsg, strongly inhibited the transcription of all adenovirus early genes, including e1a. this inhibition was not observed with wild-type pseudorabies virus coinfection or with tsg coinfection at the permissive temperature. the level of repression was dependent upon the ratio of tsg to adenovirus in the infection. the results sug ...	1986	3001339
intestinal bacterial flora and host defense mechanisms.	the protective mechanisms of intestinal bacterial flora against exogenous infections will be discussed in this paper. experimental data on protective function of escherichia coli, enterococcus faecalis and bacteroides distasonis comprising intestinal flora against oral infection of shigella flexneri which causes localized infection are presented. furthermore, the present investigation deals with non-specific defense mechanism which indicate that protective activity of intestinal flora against pa ...	1986	2968005
different classes of antibody activities to trichosporon cutaneum antigen in summer-type hypersensitivity pneumonitis by enzyme-linked immunosorbent assay.	to assess the diagnostic value of antibody activities to trichosporon cutaneum antigen in patients with summer-type hypersensitivity pneumonitis in japan, we measured the serum igg, iga, and igm antibodies to saline-soluble antigen of homogenized t. cutaneum cells by enzyme-linked immunosorbent assay (elisa). the igg antibody activity was observed in serum samples from 25 (83%) of the 30 patients and in 9 (82%) of the 11 asymptomatic family members. the iga antibody activity was found in 25 (83% ...	1986	3079979
different classes of antibody activities to trichosporon cutaneum antigen in summer-type hypersensitivity pneumonitis by enzyme-linked immunosorbent assay.	to assess the diagnostic value of antibody activities to trichosporon cutaneum antigen in patients with summer-type hypersensitivity pneumonitis in japan, we measured the serum igg, iga, and igm antibodies to saline-soluble antigen of homogenized t. cutaneum cells by enzyme-linked immunosorbent assay (elisa). the igg antibody activity was observed in serum samples from 25 (83%) of the 30 patients and in 9 (82%) of the 11 asymptomatic family members. the iga antibody activity was found in 25 (83% ...	1986	3079979
effect of guinea pig or monkey colonic mucus on shigella aggregation and invasion of hela cells by shigella flexneri 1b and 2a.	the effects of guinea pig and rhesus monkey colonic mucus preparations on shigella aggregation and invasion of hela cell monolayers by shigella flexneri serotype 1b, 2a, and 5 strains were investigated. guinea pig mucus caused agglutination of s. flexneri serotype 1b but not of s. flexneri serotype 2a or 5. guinea pig mucus also inhibited hela cell invasion by s. flexneri serotypes 1b and 2a. monkey mucus neither agglutinated any shigella strain nor inhibited hela cell invasion.	1986	3081449
characterization of the membrane-bound protein kinase c and its substrate proteins in canine cardiac sarcolemma.	cardiac sarcolemma was purified from canine ventricles. enrichment of the sarcolemmal membranes was demonstrated by the high (na+ + k+)-atpase activity of 28.0 +/- 1.5 mumol pi/mg protein per h and the high concentration of muscarinic receptors with the bmax of 8.2 +/- 2.5 pmol/mg protein as determined by [3h]qnb binding. the purified sarcolemma also contains significant levels of a membrane-bound ca2+ and phospholipid-dependent protein kinase (protein kinase c). to elucidate the protein kinase ...	1986	3082370
[use of bladder biopsy in the study of urinary schistosomiasis].	it is common to find schistosoma eggs in urine, faeces, and biopsies from rectal mucus membranes presently in use. it is less common to observe adults in routine bladder biopsies. the authors report such a case and discuss the importance of the bladder biopsy to estimate tissular inflammatory response which could reflect the host-parasite balance.	1986	3084108
disialoganglioside gd2 distributes preferentially into substrate-associated microprocesses on human melanoma cells during their attachment to fibronectin.	human melanoma cells (m21) actively attach and spread on a fibronectin substrate. indirect immunofluorescence assays with specific monoclonal antibodies directed to the disialoganglioside gd2, the major ganglioside expressed on m21 melanoma cells, indicate that during the cell attachment process this molecule redistributes into microprocesses that make direct contact with the fibronectin substrate. scanning and transmission immunoelectron microscopic studies with anti-gd2 monoclonal antibodies a ...	1986	3084502
disialoganglioside gd2 distributes preferentially into substrate-associated microprocesses on human melanoma cells during their attachment to fibronectin.	human melanoma cells (m21) actively attach and spread on a fibronectin substrate. indirect immunofluorescence assays with specific monoclonal antibodies directed to the disialoganglioside gd2, the major ganglioside expressed on m21 melanoma cells, indicate that during the cell attachment process this molecule redistributes into microprocesses that make direct contact with the fibronectin substrate. scanning and transmission immunoelectron microscopic studies with anti-gd2 monoclonal antibodies a ...	1986	3084502
endogenous luteinizing hormone surge in women during induction of multiple follicular development with pulsatile follicle stimulating hormone.	in this study nine consecutive normally cycling women undergoing in-vitro fertilization (ivf) were superovulated with clomiphene citrate followed by pulsatile 'pure' fsh injected s.c. via a pump (28 iu every 3 h). all women displayed an endogenous lh surge, which was markedly attenuated in most of the cases (peak value 44.5 +/- 5.9 u/l, duration 29.2 +/- 1.2 h, mean +/- sem) as compared to spontaneous cycles. an increase in serum progesterone levels before the onset of the lh surge was seen in o ...	1986	3085995
natural and recombinant interferons inhibit epithelial cell invasion by shigella spp.	the effect of natural and recombinant interferons (ifns) on the abilities of shigella flexneri, s. sonnei, and salmonella typhimurium to invade different human and murine cells was examined. pretreatment of cell monolayers with natural and recombinant ifns reduced the number of shigella-infected cells in a dose-dependent manner. establishment of an anti-invasive cellular state was time dependent, requiring 10 h for 50% inhibition of bacterial invasion. the inhibitory effect of ifn was species sp ...	1986	3086234
measurement of phospholipid turnover in cultured hormone responsive pituitary cells.		1986	3086663
genetic basis for altered idiotype expression in the hyperimmune response to (4-hydroxy-3-nitrophenyl)acetyl hapten.	to assess the significance of somatic point mutation in the hyperimmune response to the hapten np, an in vivo enrichment procedure was followed. mice that expressed high titers of b1-8 idiotopic determinants were selected as donors for serial transfer of small numbers of immune spleen cells into syngeneic irradiated recipient mice. cells expressing b1-8 idiotopic determinants were chosen for enrichment because b1-8 cross-reactive determinants constitute a significant portion of the primary respo ...	1986	3088116
the effect of the beta-carboline fg 7142 on the behaviour of male rats in a living cage: an ethological analysis of social and nonsocial behaviour.	the effects of fg 7142, a beta-carboline benzodiazepine receptor partial inverse agonist, on the social behavior of pair-housed rats were investigated. four 6-min dyadic social encounters in a living cage were observed in a paradigm in which one member of a pair of rats was injected. the four injection groups (n = 8) were vehicle control, and fg 7142 at 2.5, 5.0 and 10.0 mg/kg, respectively. all injections were administered 2 min before the start of the first observation trial. compared to the e ...	1986	3088637
study of aldose reductase inhibition in intact lenses by 13c nuclear magnetic resonance spectroscopy.	carbon-13 nuclear magnetic resonance spectroscopy has been used in the study of glucose metabolism, specifically aldose reductase inhibition, in intact rabbit lenses maintained in organ culture. this technique provides an effective method of screening potential inhibitors of aldose reductase under conditions that more closely approximate in vivo conditions than do earlier methods. the aspirin substitutes acetaminophen and ibuprofen were studied as aldose reductase inhibitors and were found to be ...	1986	3088727
disopyramide phosphate effects on slow and depressed fast responses.	we studied the effects of disopyramide phosphate on explanted neonatal rat ventricle cells exhibiting depressed fast responses or naturally occurring slow response action potentials together with automatic activity. disopyramide suppressed the spontaneous activity at a concentration of 2.5 micrograms/ml with a half-maximal value of 10 micrograms/ml. before spontaneous activity was lost, there was an increase in beating rate possibly related to membrane depolarization. in depressed fast and slow ...	1986	3089566
[cell proliferation in the carcinogenetic processes of tongue carcinoma induced by 4-nitroquinoline-1-oxide in rats. 1. relation between morphology and microvascular architecture of the filiform papillae of dorsal surface of the tongue in normal albino rats].		1986	3090703
chloroalanyl antibiotic peptides: antagonism of their antimicrobial effects by l-alanine and l-alanyl peptides in gram-negative bacteria.	a large number of structurally diverse di- and tripeptides containing the alanine racemase inactivator beta-chloro-l-alanine (beta-cl-lala) have been synthesized, and their antibacterial properties in vitro have been evaluated. the dipeptides 1, 3-6, and 8-17 and the tripeptide 20 are all broad-spectrum antibacterial agents with considerable potency against both gram-positive and gram-negative species, but none of these peptides improves dramatically on the antibiotic efficacy of the previously ...	1986	3093682
noradrenergic and opioid mediation of tricyclic-induced reversal of escape deficits caused by inescapable shock pretreatment in rats.	the present study was undertaken to investigate the possible involvement of a noradrenergic and opioid mediation of the reversal by tricyclic antidepressants of escape deficits produced by inescapable shock pretreatment. rats were first exposed to 60 inescapable shocks (15 s duration, 0.8 ma, every min +/- 15 s) and 48 h later subjected, to daily shuttle-box sessions (30 trials/day, iti: 30 s) during 3 consecutive days. twice-daily ip injection of desipramine or clomipramine (total daily dose: 3 ...	1986	3094068
[prevalence of campylobacter jejuni complement fixing antibody in healthy people].		1986	3097197
rash mutants deficient in gtp binding.	single amino acid substitutions were introduced into a region of the rash protein (residues 116, 117, and 119) homologous to a variety of diverse gtp-binding proteins. each of the mutant p21 proteins displayed a significant reduction (10- to 5,000-fold) in gtp binding affinity. activated rash proteins deficient in gtp binding were unaltered in their ability to morphologically transform nih 3t3 cells.	1986	3097518
signals in the phi 29 dna-terminal protein template for the initiation of phage phi 29 dna replication.	the protein-free terminal fragments hindiii b and l, from the left and right ends of phi 29 dna, respectively, but not internal fragments of similar size, were active as templates in the formation of the p3-damp initiation complex in an in vitro system containing purified phi 29 terminal protein p3 and dna polymerase p2, although the activity was lower than that obtained with the phi 29 dna-p3 complex. these results indicate the existence of specific sequences at the ends of phi 29 dna that allo ...	1986	3097958
radioimmunoscintigraphy of human gastric carcinoma xenografts with 125i-labeled monoclonal antibody.	a monoclonal antibody (gc 302) established in our laboratory, which was reactive with gastric carcinoma and other epithelial carcinoma but not with normal gastric mucosa or other malignant tumors of mesenchymal origin, was used to investigate the radioimmunolocalization of tumors. various kinds of target cells (5 x 10(5)) were incubated with 125i-labeled gc 302, and radioactivity was determined with a gamma counter. it was shown that there was a 500- to 1,000-fold increase in counts for gastric ...	1986	3098721
characterization and diagnostic application of a lipopolysaccharide core oligosaccharide-protein conjugate.	shigella sonnei phase ii core oligosaccharide (osphii) was linked covalently with protein (bovine serum albumin or tetanus toxoid) to obtain a conjugate (osphii-protein) of good immunogenicity in rabbits. anti-osphii-protein conjugate sera contained high levels of immunoglobulin g antibodies against the complete core region of sh. sonnei lipopolysaccharide. the antigenic relationships between lipopolysaccharides of r1, r3 and r4 core types using anti-osphii-protein sera were studied. these antis ...	1986	3098854
effect of measles-virus infection and interferon treatment on invasiveness of shigella flexneri in hep2-cell cultures.	the influence of measles-virus infection on the invasiveness of shigella flexneri in hep2-cell cultures was studied. bacterial invasiveness was significantly enhanced in cell cultures incubated with virus before bacterial inoculation. this effect was a function of time after introduction of virus to the cell cultures and of the concentration of virus. the increase in bacterial invasiveness was observed before production of infectious virus particles and before a cytopathic effect was evident. a ...	1986	3098977
separation and quantification of prostaglandins e1 and e2 as their panacyl derivatives using reverse phase high pressure liquid chromatography.	separation and quantification of prostaglandin e1 (pge1) and prostaglandin e2 (pge2) were achieved using reverse phase high performance liquid chromatography (hplc). panacyl bromide (p-(9-anthroyloxy)phenacyl bromide) (pab) derivatives of pge2 and pge1 were prepared. reverse phase hplc using a linear gradient of 56% to 80% acetonitrile in water containing 0.10% acetic acid gave baseline resolution of the two derivatives. a 3 um diameter particle, c18 column provided good resolution and reproduci ...	1986	3099332
a strain of multiform cryptococcus neoformans.		1986	3100152
salicylates inhibit paf-acether-induced rat paw oedema when cyclooxygenase inhibitors are ineffective.	the cyclooxygenase inhibitors indomethacin, piroxicam, ibuprofen, naproxen and flurbiprofen failed to block rat paw oedema induced by paf-acether, whereas aspirin and sodium salicylate were effective. two mixed cyclooxygenase and lipoxygenase inhibitors ndga, bw 755c and dexamethasone reduced oedema in a dose - dependently. the selective paf-acether antagonist, bn 52021, was effective against paf-acether at 5 - 20 mg/kg. the lipoxygenase derivates may be involved in paw oedema induced by paf-ace ...	1986	3103172
[influence of bcg in the control of humoral deficiency induced by mastocytoma p-815].	viable bcg bacilli, in a lipid emulsion, inoculated intravenously, were capable of reverting the profound humoral immunosuppression induced in adult dba/2 mice by the mastocytoma p-815. bcg increased not only the number of hemolytic plaque forming cells, but also the serum titers of hemagglutinating igm antibodies. however, no blocking effect was detected on normal tumor progression.	1986	3108617
immunological relationship among glycogen-protein complexes of different species.	glycogen-protein complexes from the muscles of celerio euphorbiae and horse as well as from the livers of frog, carp and man were isolated and used as antigens. after immunization of rabbits antisera were obtained from which fractions of immunoglobulins were isolated. antibodies were used in agar immunodiffusion tests and in quantitative reaction of immunoprecipitation with glycogens. the immunoprecipitation test proved the occurrence of quantitative differences in cross reaction of complexes wi ...	1986	3109350
hormonal control of polyamine levels in bovine adrenocortical cells.	the implication of polyamines in cellular growth and differentiation processes and the existence of a polyamine-mediated protein phosphorylation system in adrenocortical cells suggest that polyamines may be examined as potential intracellular messengers in the pleiotypic action of acth. bovine adrenocortical cells in culture exhibit a specific, energy-dependent, partly sodium-supported, inward polyamine transport system, independent of the a, l, and n aminoacid uptake systems. steroidogenic conc ...	1986	3004891
studies on the glycoprotein nature of the thyrotropin receptor: interaction with lectins and purification of the bovine protein with the use of bandeiraea (griffonia) simplicifolia i affinity chromatography.	the tsh receptor from triton-solubilized bovine microsomal membranes was found to bind to a substantial extent to columns of the immobilized lectins bandeiraea (griffonia) simplicifolia i, ricinus communis i, wheat germ, and concanavalin a, whereas it was not retained by dolichos biflorus. elution of tsh receptor activity from these lectins could be achieved with the appropriate saccharides in all cases except concanavalin a. the most extensive adsorption of the receptor occurred on b. simplicif ...	1986	3004903
[similar organization of beta,beta'-subunit rna-polymerase genes and adjacent ribosomal protein genes in enterobacteriaceae and pseudomonas putida].	the genes coding for the rna-polymerase beta,beta'-subunits and adjacent ribosomal protein genes in escherichia coli, salmonella typhimurium, shigella flexneri, serratia marcescens, proteus mirabilis and pseudomonas putida are compared by the southern hybridization procedure. in all the species studied close clustering of the genes rplkajl and rpobc is demonstrated. preliminary physical maps for these genes in s. typhimurium, s. flexneri, s. marcescens and p. mirabilis are proposed. rifampicin i ...	1986	3005845
enteropathogens associated with acute diarrhea in hospitalized infants.	thirty-five infants of low socioeconomic status who were living in urban santiago were hospitalized for acute diarrhea were prospectively evaluated for the presence of enteropathogens associated with the episode. some degree of malnutrition was evident in 20 infants (57.1%); 15 of these (75%) were under 6 months of age. mean duration of the hospital stay was 11.8 days for well-nourished patients and 15.7 days for the malnourished patients. one or more enteropathogens were found in 60% of the cas ...	1986	3007718
complete dissociation between the activation of phosphoinositide turnover and of nadph oxidase by formyl-methionyl-leucyl-phenylalanine in human neutrophils depleted of ca2+ and primed by subthreshold doses of phorbol 12,myristate 13,acetate.	evidences have been provided by many laboratories that the activation of the nadph oxidase in neutrophils by formyl-methionyl-leucyl-phenylalanine (fmlp) is strictly linked to a transduction pathway that involves the stimulation, via gtp binding protein, of the phosphoinositide turnover and the increase in [ca2+]i. the results presented in this paper demonstrate that fmlp can activate the nadph oxidase by triggering a transduction pathway completely independent of phosphoinositide turnover and c ...	1986	3008746
identification by antibody to a synthetic peptide of a protein specified by a diploid gene located in the terminal repeats of the l component of herpes simplex virus genome.	in the course of studies on the a sequences located at the termini of and at the junction between the l and s components of herpes simplex virus 1 dna, j. chou and b. roizman (j. virol. 57:629-637, 1986) noted that the a sequence acted as a gamma 1 promoter when fused to the structural sequence of the thymidine kinase gene, the b inverted repeat sequences located in the l component next to the a sequences contained an open reading frame predicted to encode the protein of 358 amino acids with a m ...	1986	3009891
radioreceptor assay of anti-tsh receptor antibody activity: comparison of assays using unextracted serum and immunoglobulin fractions, and standardization of expression of activities.	a radioreceptor assay for tsh receptor antibodies developed by shewring and smith is described in which unextracted serum is used. the assay is simple and reproducible. tsh-binding inhibitor immunoglobulin (tbii) activity determined using unextracted serum correlated well with that determined using the immunoglobulin fraction purified with polyethylene glycol. the assay detected tsh receptor antibodies in 81 of 85 patients (95%) with untreated graves' disease, no patients with graves' disease in ...	1986	3016274
anti-htlv-iii screening of blood donors.	in a survey on 82,383 blood donor specimens from hessen, west germany, the observed percentage of repeatedly positive htlv-iii/lav antibody elisa results was 0.2%, but only 0.018% were confirmed by western blot, immunofluorescence assay or radioimmunoprecipitation assay. these data are consistent with larger epidemiological data compiled from other german blood banks and suggest a high degree of nonspecific false-positive results; one of the causes for false-positives are hla-dr4 antibodies, whi ...	1986	3017001
effects of theophylline and camp on amphiuma small intestine.		1986	3017122
effect of age on the formation of cyclic nucleotides in guinea-pig tracheal smooth muscle in response to pharmacological agents.	the effects of isoproterenol, carbachol and other drugs on the cyclic amp and cyclic gmp levels in tracheal smooth muscles of guinea-pigs of four different ages were investigated. isoproterenol increased the cyclic amp level several-fold in tracheal muscles from newborn (1 week) and young (4-7 weeks) guinea-pigs, but it caused less increase in the level in muscles from middle-aged (12 weeks) and old (20-24 weeks) guinea-pigs, although the basal cyclic amp level at these ages was not significantl ...	1986	3017724
steroid-responsive bronchiolitis after human heart-lung transplantation.	this report describes the clinical course of a patient who developed obliterative bronchiolitis after viral infection on three separate occasions. long-term follow-up is given. it is suggested that the syndrome of late pulmonary deterioration after transplantation may be steroid responsive if treatment is initiated early in the natural history of the syndrome. in addition, it is suggested that increased emphasis should be placed on the documentation of viral infection in transplant recipients to ...	1986	3018383
testing for htlv-iii/lav antibody.		1986	3019171
studies on the antihypertensive effect of single doses of the angiotensin converting enzyme inhibitor ramipril (hoe 498) in man.	the time course of the blood pressure lowering effect and the dose-response relationship of the new angiotensin converting enzyme inhibitor ramipril (hoe 498) were studied in 8 patients, with essential hypertension. as compared with placebo, a single oral dose of 2.5 mg ramipril lowered systolic and diastolic blood pressure. the antihypertensive action of single oral doses of 5, 7.5 and 10 mg ramipril was more pronounced. no change in heart rate occurred. angiotensin converting enzyme activity w ...	1986	3019702
studies on the antihypertensive effect of single doses of the angiotensin converting enzyme inhibitor ramipril (hoe 498) in man.	the time course of the blood pressure lowering effect and the dose-response relationship of the new angiotensin converting enzyme inhibitor ramipril (hoe 498) were studied in 8 patients, with essential hypertension. as compared with placebo, a single oral dose of 2.5 mg ramipril lowered systolic and diastolic blood pressure. the antihypertensive action of single oral doses of 5, 7.5 and 10 mg ramipril was more pronounced. no change in heart rate occurred. angiotensin converting enzyme activity w ...	1986	3019702
animal models for retrovirus-induced immunodeficiency disease.		1986	3019879
animal models for retrovirus-induced immunodeficiency disease.		1986	3019879
isolation, characterization, and mapping of tn5 insertions into the 140-megadalton invasion plasmid defective in the mouse sereny test in shigella flexneri 2a.	using shigella flexneri 2a ysh6000, we isolated 304 independent tn5 insertion mutants in the 230-kilobase invasion plasmid, pmysh6000. the site of each tn5 insertion was assigned to 23 sali fragments on the previously made sali cleavage map of pmysh6000. among the 304 insertions, 150 were negative in expression of four phenotypes examined (mouse sereny test [ser], invasion into epithelial cells [inv], congo red binding [pcr], and inhibition of bacterial growth [igr] ): 12 were ser- inv+ pcr+ igr ...	1986	3019891
immortalization of human t lymphocytes by htlv-i: phenotypic characteristics of target cells and kinetics of virus integration and expression.	in-vitro infection of normal human lymphocytes with htlv-i (human t-cell lymphotropic retrovirus type i) has been carried out to study the target cell specificity and the kinetics of infection. cord blood (cbl) and adult peripheral blood lymphocytes (pbl) have been co-cultivated with irradiated htlv-i donor cells (mt2 and c91pl lines). established ('immortalized') htlv-i positive cell lines were obtained only from cbl: in comparison with pbl, a less mature phenotype of t-cell subsets and a lower ...	1986	3020320
substrate phosphoprotein availability regulates eclosion hormone sensitivity in an insect cns.	the final step in the moulting of all insects is ecdysis, the shedding of the cuticle of the previous instar, which is triggered in lepidoptera by the neurosecretory peptide eclosion hormone. this hormone acts directly on the nervous system to release the appropriate motor patterns for larval, pupal and adult ecdysis, but there are only brief periods near the end of each moult when the nervous system is competent to respond to the hormone. previous experiments have shown that the action of eclos ...	1986	3020427
substrate phosphoprotein availability regulates eclosion hormone sensitivity in an insect cns.	the final step in the moulting of all insects is ecdysis, the shedding of the cuticle of the previous instar, which is triggered in lepidoptera by the neurosecretory peptide eclosion hormone. this hormone acts directly on the nervous system to release the appropriate motor patterns for larval, pupal and adult ecdysis, but there are only brief periods near the end of each moult when the nervous system is competent to respond to the hormone. previous experiments have shown that the action of eclos ...	1986	3020427
differential effects of transforming growth factor type beta on the growth and function of adrenocortical cells in vitro.	transforming growth factor type beta (tgf-beta) suppresses basal as well as corticotropin (acth)-stimulated steroid formation by bovine adrenocortical cells in culture. the effect is dose dependent and is not accompanied by any change in adrenocortical cell growth. the minimum effective dose of tgf-beta is 4 x 10(-13) m (10 pg/ml), and maximal inhibition is observed at a concentration of 4 x 10(-11) m (1 ng/ml). a 16- to 20-hr incubation with tgf-beta is required to decrease steroidogenesis, and ...	1986	3020557
differential effects of transforming growth factor type beta on the growth and function of adrenocortical cells in vitro.	transforming growth factor type beta (tgf-beta) suppresses basal as well as corticotropin (acth)-stimulated steroid formation by bovine adrenocortical cells in culture. the effect is dose dependent and is not accompanied by any change in adrenocortical cell growth. the minimum effective dose of tgf-beta is 4 x 10(-13) m (10 pg/ml), and maximal inhibition is observed at a concentration of 4 x 10(-11) m (1 ng/ml). a 16- to 20-hr incubation with tgf-beta is required to decrease steroidogenesis, and ...	1986	3020557
exposure of sheep to natural aerosols of foot-and-mouth disease virus.	sheep taken individually and allowed to inhale air being drawn along a duct from a cabinet containing pigs acutely infected with foot-and-mouth disease virus for 10 or 15 minute periods were infected by doses as low as 10 tcid50 of virus. the most consistent and reliable indicators of infection were viraemia and seroconversion. the mean times from exposure to onset of viraemia, pyrexia and the appearance of vesicular lesions were 2.5, 3.8 and 4.7 days, respectively. neither the time from exposur ...	1986	3020658
diminishing morbidity and mortality of bone marrow transplantation.	the reasons for the operational failure of bone marrow transplantation (bmt) are familiar and varied. they are the following: nonmarrow toxicity of the preparative regimen, marrow toxicity of the preparative regimen, acute graft-versus-host disease (gvhd), opportunistic infections, especially viral infections and recurrence of hematologic malignancy in the case of transplants for leukemia. this review examines operational failure of bmt and presents measures for its prevention.	1986	3020798
dna sequence and product analysis of the virf locus responsible for congo red binding and cell invasion in shigella flexneri 2a.	the dna sequence of virf, a locus associated with virulence and the ability to bind congo red in shigella flexneri 2a that is located on a 140-megadalton (230-kilobase) plasmid, was determined and analyzed. it was rich in a and t. the direction of transcription of virf was determined by using a chloramphenicol resistance cartridge. an open reading frame readable in this direction was found. three proteins, 30, 27, and 21 kilodaltons, all corresponding to those predicted from the above sequence, ...	1986	3021627
characterization of shigella flexneri sequences encoding congo red binding (crb): conservation of multiple crb sequences and role of is1 in loss of the crb+ phenotype.	the ability to bind congo red (crb+) is associated with virulence of shigella flexneri and is encoded by a large, 220-kilobase plasmid. we cloned fragments of this plasmid to isolate the sequences encoding congo red binding, to determine the degree of conservation of these sequences among s. flexneri strains, and to study the molecular basis for loss of the crb+ phenotype. at least two separate bamhi fragments cloned into plasmid vectors encode congo red binding in e. coli or s. flexneri. one cr ...	1986	3021630
human leukocyte antigens and sister chromatid exchanges in families with multiple adenomatosis coli.	to study human leukocyte antigens (hla) and sister chromatid exchanges (sce) in multiple adenomatosis coli (mac), eight families were evaluated. twenty-five subjects (14 affected members and 11 healthy relatives) were studied with hla a, b, and c typing of peripheral lymphocytes and sce count in cells that had replicated twice. the results of the present immunogenetic study, although limited to a small number of cases, suggest a correlation between hla, sce, and mac. when the results of further ...	1986	3022927
role of leukotrienes in rat reversed passive arthus pleurisy and the effect of aa-861, a 5-lipoxygenase inhibitor.	in studies of the role of leukotrienes in inflammatory reactions, the induction of rat reversed passive arthus pleurisy (a type iii allergic reaction) was employed. increases of exudate volume, vascular permeability, and migration of inflammatory cells in the pleural cavity were observed. the vascular permeability was enhanced biphasically during 0-30 min (early response) and during 3-6 h (late response) after induction of the pleurisy. the infiltration of inflammatory cells, mainly polymorphonu ...	1986	3000949
interaction studies in mice of sch 29851, a potential non-sedating antihistamine, with commonly used therapeutic agents.	sch 29851 was studied for its ability to interact with selected drugs used in man. the non-sedating antihistamine terfenadine and the sedating antihistamine diphenhydramine were also studied for comparison. sch 29851 at 80 mg/kg po in mice, a dose about 50 times its ed50 for blocking histamine-induced paw edema in the same species, potentiated the anticonvulsant effects of diazepam. at the high dose of 320 mg/kg po, about 80 times its ed50 for antihistamine effects, sch 29851 potentiated the abi ...	1986	2945410
effect of mononuclear cell factors on calcium transport in sarcoplasmic reticulum vesicles.	the effect of supernatants from cultures of mitogen-stimulated human mononuclear cells on calcium transport by sarcoplasmic reticulum was examined. calcium transport was assayed by measuring the time course of calcium accumulation by sarcoplasmic reticulum incubated with supernatants from stimulated mononuclear cells was 20% less than that by vesicles exposed to control supernatants (p less than 0.001). in contrast, no difference in calcium-dependent atpase activity was noted between vesicles in ...	1986	2946307
structure-activity relationships of sex steroid analogs determined in vitro in a thyroid hormone-responsive membrane ca2+-atpase model.	physiological concentrations of l-t4 were found previously to stimulate ca2+-atpase activity in vitro in reticulocyte membranes from female rabbits and to inhibit this enzyme in reticulocyte membranes from males. in these previous studies, preincubation of intact cells or ghosts with testosterone (5 x 10(-11) m) converted female-source reticulocyte membranes to male-type responsiveness to thyroid hormone (inhibition of ca2+-atpase activity). preincubation of reticulocyte membranes with 17 beta-e ...	1986	2946570
structure-activity relationships of sex steroid analogs determined in vitro in a thyroid hormone-responsive membrane ca2+-atpase model.	physiological concentrations of l-t4 were found previously to stimulate ca2+-atpase activity in vitro in reticulocyte membranes from female rabbits and to inhibit this enzyme in reticulocyte membranes from males. in these previous studies, preincubation of intact cells or ghosts with testosterone (5 x 10(-11) m) converted female-source reticulocyte membranes to male-type responsiveness to thyroid hormone (inhibition of ca2+-atpase activity). preincubation of reticulocyte membranes with 17 beta-e ...	1986	2946570
docosahexaenoic acid status of preterm infants at birth and following feeding with human milk or formula.	the docosahexaenoic acid (dha) status of preterm infants (less than 32 wk gestation) was measured as the molar percent of dha in individual red blood cell phospholipids: 1) in cord venous blood immediately following delivery, 2) after infants were receiving greater than 60 kcal x kg x day of energy from oral-gastric feedings, and 3) at a mean of 7 wk later. infants on full feeding received either preterm human milk or formula. the dha concentration of all phospholipid classes declined between bi ...	1986	2947455
[effects of my-5116 on experimental animal models of type i-type iv allergic reactions and the formation of ige antibody].	the effects of a new anti-allergic agent, my-5116: isoamyl 5,6-dihydro-7,8-dimethyl-4,5-dioxo-4h-pyrano [3,2-c] quinoline-2-carboxylate, on experimental animal models of type i approximately type iv allergic reactions and the formation of ige antibody were investigated. my-5116 administered intraperitoneally at doses of 30 and 100 mg/kg suppressed significantly the homologous pca in guinea pigs. my-5116 administered intraperitoneally at the dose of 100 mg/kg also suppressed significantly the het ...	1986	2947841
cnbr cleavage of the light chain of human complement factor i and alignment of the fragments.	the light chain and heavy chain of reduced and alkylated human complement factor i were purified by high-pressure gel-permeation chromatography. cnbr cleavage of factor i light chain yielded four major fragments, which were purified by gel filtration. n-terminal sequence analysis of the cnbr-cleavage fragments allowed identification of 200 of the approx. 240 amino acid residues of the light chain. an alignment is proposed, based on sequence analysis of peptides obtained after cleavage at arginin ...	1986	2937401
effects of heparan sulfate removal on attachment and reattachment of fibroblasts and endothelial cells.	human skin fibroblasts and calf aorta endothelial cells were grown as tissue culture monolayers in the presence of [35s]sulfate in order to label the glycosaminoglycan portions of proteoglycans for investigation of their role in cell attachment. the [35s]glycosaminoglycans were then selectively removed from the cell monolayers by the addition of various glycosaminoglycan-degrading enzymes. as previously described, in contrast to trypsin treatment none of these enzymes removed any cells from the ...	1986	2937448
chromophore/protein interaction in bacterial sensory rhodopsin and bacteriorhodopsin.	retinal analogues with altered conjugated double bond systems or altered stereochemistry were incorporated into the phototaxis receptor sensory rhodopsin (sr) and the light-driven proton pump bacteriorhodopsin (br) from halobacterium halobium. wavelength shifts in absorption ("opsin shifts") due to analogue interaction with the protein microenvironment demonstrate that the same overall electrostatic and steric properties of the retinal binding-site structures exist in both proteins despite their ...	1986	2937462
cultured human endothelial cells synthesize a plasma membrane protein complex immunologically related to the platelet glycoprotein iib/iiia complex.	we have previously demonstrated that endothelial cells synthesize a plasma membrane protein indistinguishable from platelet glycoprotein (gp) iia. the present study provides evidence for a further analogy between the platelet and the endothelial cell membrane by showing that cultured endothelial cells also synthesize a membrane protein complex immunologically related to the platelet gp iib/gp iiia complex. this evidence is based on the following observations: (1) c17, a murine monoclonal antipla ...	1986	2937471
a follow up of t-cell subsets and of anti-m. leprae antibody titer as measured by the fla-abs test in melanesian leprosy patients under polychemotherapy.	melanesian leprosy patients from new caledonia were studied for the following parameters during the course of polychemotherapy: peripheral blood t-cell subsets, as identified in an immunofluorescence assay with monoclonal antibodies okt3 ("pan-t"), okt4 ("helper/inducer"), and okt8 ("cytotoxic-suppressor"), and anti-mycobacterium leprae antibodies in the serum, as measured by the fluorescent leprosy antibody absorption test. a group of melanesian healthy subjects with no known exposure to m. lep ...	1986	2940306
[risk factors in the epidemiology of dysentery].	the general tendency of a decrease in the morbidity rates of dysentery induced by shigella sonnei and shigella flexneri (separately) from monday to the end of the week (saturday-sunday) has been revealed, and at the same time the "infection risk" for both kinds of salmonellosis has been found to fall on the last days of the week (friday-sunday).	1986	2940778
dose-related effects of synthetic human beta-endorphin and naloxone on fed gastrointestinal motility.	in humans, plasma beta-endorphin levels rise during application of acute stressful stimuli (vertigo, cold pain, and transcutaneous electrical stimulation) that induce gut motor disturbances. whereas it is possible that circulating beta-endorphin participates in the mediation of these central effects on gut motility, its role cannot be established solely on the basis of changes in plasma levels. therefore, we designed the present study to investigate 1) the dose-related effects of intravenous syn ...	1986	2942039
isolation, characterization and utilization of the fifth component (c5) of porcine complement.	the fifth component (c5) of porcine complement was isolated in immunochemically pure, homogeneous and native form. the isolated component was characterized, it has a molecular weight of 187,000 (under non-reducing conditions) and two non-identical chains of 112,000 and 76,000 under reducing conditions, respectively. the isolated c5 can be used for the detection of the c5b receptors on murine macrophages. our findings are in good agreement with the results obtained using a rabbit and a human mode ...	1986	2943607
ultracytochemical evidence for a proton-pump adenosine triphosphatase in chick osteoclasts.	the distribution of mg++-atpase in osteoclasts along the endosteal surface of the chick tibia was investigated by neutral and alkaline ph cytochemical methods at the electron-microscopic level. reaction product was observed in mitochondria, cytoplasmic vesicles, and ruffled-border membrane. levamisole, ouabain, and vanadate did not affect the enzymatic activity. para-chloromercuribenzoic acid (pcmb) prevented staining of mitochondria, ruffled border, and most cytoplasmic vesicles. tri-n-butyltin ...	1986	2944590
total iridectomy does not alter outflow facility responses to cyclic amp in cynomolgus monkeys.	total outflow facility was measured by two-level constant pressure anterior chamber perfusion in surgically untouched and totally iridectomized cynomolgus monkey eyes receiving bolus intracameral infusions of cyclic amp, dibutyryl cyclic amp, or 5'amp. cyclic amp doses of 50 and 100 micrograms (corresponding to initial intracameral concentrations of 1.5- and 3 mm) increased facility by approximately 20 and 40% respectively in both surgically untouched and aniridic eyes. higher doses produced no ...	1986	3023122
a conserved human germline v kappa gene directly encodes rheumatoid factor light chains.	the full-length gene that encodes the light chain variable regions of an idiotypically related group of human igm kappa rheumatoid factors (rfs) has been cloned and sequenced. the deduced amino acid sequence is identical to four separate rf proteins. these results prove that genes capable of encoding human anti-igg autoantibody light chains without any somatic mutation are present in the kappa gene repertoire of normal people.	1986	3023521
a conserved human germline v kappa gene directly encodes rheumatoid factor light chains.	the full-length gene that encodes the light chain variable regions of an idiotypically related group of human igm kappa rheumatoid factors (rfs) has been cloned and sequenced. the deduced amino acid sequence is identical to four separate rf proteins. these results prove that genes capable of encoding human anti-igg autoantibody light chains without any somatic mutation are present in the kappa gene repertoire of normal people.	1986	3023521
structure of replicating simian virus 40 minichromosomes. the replication fork, core histone segregation and terminal structures.	the structure of replicating simian virus 40 (sv40) minichromosomes was studied by dna crosslinking with trimethyl-psoralen. the procedure was used both in vitro with extracted sv40 minichromosomes as well as in vivo with sv40-infected cells. both procedures gave essentially the same results. mature sv40 minichromosomes are estimated to contain about 27 nucleosomes (error +/- 2), except for those molecules with a nucleosome-free gap, which are interpreted to contain 25 nucleosomes (error +/- 2). ...	1986	3023620
relative activities of 5'-nucleotidase and adenosine deaminase in atrial and ventricular myocardium--the enzyme paradox.	the 5'-nucleotidase and adenosine deaminase activities were determined for tissue extracts of up to 10 defined regions of the normal dog heart. the specific activity of 5'-nucleotidase was very significantly higher in atrial than in ventricular myocardium (4 x) and was also higher in right than in left sites of the heart (2 x). the reason for the markedly higher specific activity in atrial muscle is not clear and appears paradoxical relative to adenine nucleotide content and pattern of atp decay ...	1986	3025011
structurally related bacillus thuringiensis delta-endotoxins display major differences in insecticidal activity in vivo and in vitro.	many strains within the 22 serotypes of bacillus thuringiensis produce crystal delta-endotoxins with slight differences in their insecticidal toxicity spectrum in vivo. since the basis of this specificity is unknown, we chose to compare the activity of delta-endotoxins from three strains: b. thuringiensis var. kurstaki hd-1, var. aizawai hd-249 and var. thuringiensis hd-350, both in vivo and on insect cell lines in vitro. immunoblotting with antisera to activated var. kurstaki p1 lepidopteran to ...	1986	3027111