Publications

evaluation of a commercial elisa kit for the serological diagnosis of helicobacter pylori infection.	h. pylori infection can be diagnosed serologically. we evaluated an elisa kit (helicobacter pylori igg, diesse) prepared using a glycine extract of an autoctonous h. pylori strain which produced the highest biologically active urease titre out of five strains tested. the kit was tested with serum samples from h. pylori-infected and uninfected adults and children. the western blot technique was used as reference method for the h. pylori infective status. based on the results obtained by immunoblo ...	1994	7861988
gastroprotective agents in mucosal defense against helicobacter pylori.	1. convincing evidence now exists that infection with h. pylori plays a major role in the pathogenesis of gastric disease. having a niche bordering two major perimeters of mucosal defenses, the bacterium apparently exerts its detrimental effect on the mucus layer as well as the gastric epithelium. therefore, gastroprotective agents capable of counteracting these detrimental effects of h. pylori are gaining importance in the treatment of gastric disease. 2. the colonization of gastric mucosa by h ...	1994	7835626
inhibition of gastric mucosal mucin receptor by helicobacter pylori lipopolysaccharide: effect of sulglycotide.	1. a receptor for mucin was isolated from the solubilized gastric epithelial cell membrane by affinity chromatography on sepharose-bound wheat germ agglutinin. 2. the receptor protein displayed a molecular weight of 97 kda and exhibited specific affinity towards mucin-coated surfaces. the optimum for mucin binding occurred at 60-100 micrograms/ml, while the values for the receptor were 2.0-3.1 micrograms/ml. 3. the mucin binding to the receptor was susceptible to helicobacter pylori lipopolysacc ...	1994	7835646
observer variation in the assessment of chronic gastritis according to the sydney system.	the main aims of the sydney system for the classification of gastritis are to improve uniformity in histopathological reporting and to provide a flexible matrix of rules for grading the histological features. we sought to determine the level of interobserver agreement between pathologists in the application of the sydney system. three histopathologists independently examined h & e, alcian blue/pas and modified giemsa stained sections of two antral and two corpus gastric biopsies from 69 consecut ...	1994	7835836
a role for cd4+ t cells from orally immunized rats in enhanced clearance of pseudomonas aeruginosa from the lung.	the role of gut-derived cd4+ t cells in clearance of pseudomonas aeruginosa from the lung was studied by cell transfer experiments. mesenteric lymph node cells from unimmunized rats, or rats orally immunized with either killed p. aeruginosa or helicobacter pylori, were transferred to naive rats which were subsequently challenged intra-tracheally with live p. aeruginosa. recipients of unseparated mesenteric lymph node cells, purifed t cells or cd4+ t cells, from p. aeruginosa-immunized donors, al ...	1994	7835960
[analysis of immune response to helicobacter pylori--identification of the protein recognized by anti-helicobacter pylori antibodies from sera of patients with gastroduodenal diseases].	hp-specific antibodies were measured in each collected fraction obtained from gel-filtrated separation method with selected patients sera by enzyme-linked immunosorbent assay (elisa). most of the fractions reacted with the patients sera also responded to hp urease-specific monoclonal antibodies. in addition, the fractions which showed urease activity strongly correlated with the hp antibody positive ones. also, we could not detect any cross-reactivity to ureases of other species. moreover, most ...	1994	7837686
prevalence of helicobacter pylori antibodies in uremic patients.		1994	7838282
quality of life and relapse of duodenal ulcer before and after eradication of helicobacter pylori.	the aim of the study was to compare relapse rates and quality of life before and after eradication of helicobacter pylori in a group of patients with documented recurrent duodenal ulcer disease.	1994	7839092
intravenous omeprazole/amoxicillin and omeprazole pretreatment in helicobacter pylori-positive acute peptide ulcer bleeding. a pilot study.	the aims of this study were to evaluate a helicobacter pylori eradication schedule for h. pylori-positive gastroduodenal ulcer bleeding, which could be commenced intravenously after endoscopic diagnosis, and to assess the effect of omeprazole pretreatment on bacterial eradication.	1994	7839093
distinct profiles of gastritis in dyspepsia subgroups. their different clinical responses to gastritis healing after helicobacter pylori eradication.	a contribution of helicobacter pylori gastritis to the pathogenesis of non-ulcer dyspepsia (nud) remains uncertain.	1994	7839094
[is helicobacter pylori infection the etiology of duodenal ulcer?].		1994	7834710
standardization of disk diffusion test and its clinical significance for susceptibility testing of metronidazole against helicobacter pylori.	susceptibilities of 121 clinical helicobacter pylori strains to metronidazole were determined by both a 5-micrograms metronidazole disk diffusion test and a plate dilution method in duplicate and after different periods of incubation. the distribution of mics of metronidazole against h. pylori among the strains was found to be bimodal. the diameters of inhibitory zones obtained by the disk diffusion test and the mics obtained by the plate dilution method correlated well, especially after 4 days ...	1994	7840570
role of helicobacter pylori infection in gastro-duodenal secretion and in pathogenesis of peptic ulcer and gastritis.	etiologic role for hp appears to be best established in histologically proven gastritis. the major factors mediating gastritis induced by the colonization of the "gastric type" mucosa with hp are probably cytotoxins, cytokines and free radicals activated by this organisms. the deficiency of negative feedback in somatostatin-gastrin link in antral gastritis may result in an excessive gastrin release and increased gastric acid secretion with increased duodenal acid load under basal state and after ...	1994	7841447
antibodies against some bacterial antigens in children.	the prevalence of bacterial antibodies was determined in 173 children aged 0-15 years. the prevalence of igg borrelia burgdorferi antibodies in titres > 500 in children less than 8 years of age was 6% while none of the older children had these antibodies in titres > 400. igg helicobacter pylori antibodies were detected only in children older than 6 years of age, with a prevalence of 6.5%, as were iga h. pylori antibodies, with a prevalence of 3.7%. the prevalence of high-titre igg campylobacter ...	1994	7841725
[malt lymphoma, stomach carcinoma--role of helicobacter pylori. are chances for prevention on the horizon?].	the pathogenesis of gastric carcinoma developing after infection with helicobacter pylori now seems to be clear. the release of urease, alcohol dehydrogenase, enzymes and cytotoxin on the one hand, and chemotactic factors, paf and heat-shock proteins on the other trigger chronic inflammation and epithelial metaplasia and dysplasia in the stomach. under the influence of additional carcinogens, the epithelial changes progress to severe dysplasia and finally carcinoma. as a result of chronic inflam ...	1994	7843683
the enemy within.		1994	7844800
[an enzyme-linked immunosorbent assay for the detection of igg antibodies against urease of helicobacter pylori].	we have developed an enzyme-linked immunosorbent assay (elisa) for the detection of igg antibodies against helicobacter pylori (hp) using purified hp urease as an antigen. the urease was purified from ultrasonicated extract of hp by nacl linear gradient system on deae-sepharose 4b chromatography. two molecular weight bands, 65kd and 27kd were observed on a sds-page gel in the purified urease sample. the urease antigen did not crossreact to rabbit antibodies prepared against campylobacter coli an ...	1994	7844892
[helicobacter pylori eradication therapy with omeprazole and amoxicillin: current status].	combined therapy with the proton pump inhibitor omeprazole and amoxicillin has become an important alternative in the treatment of ulcer disease associated with helicobacter pylori infection. due to the high efficacy in eradicating h.pylori, missing resistance of h.pylori against amoxicillin and high tolerability and digestibility this regimen may be recommended for widespread routine use. in a randomized, double-blind multicenter trial an h.pylori eradication rate of over 90% has been achieved ...	1994	7845167
[cost-benefit analysis of conservative therapy of gastritis and ulcer disease].		1994	7845170
benefits from elimination of helicobacter pylori infection include major reduction in the incidence of peptic ulcer disease, gastric cancer, and primary gastric lymphoma.	it has recently been recognized that gastritis, gastric ulcer, duodenal ulcer, gastric carcinoma, and primary gastric b-cell lymphoma are all associated with gastroduodenal infection with the bacterium helicobacter pylori. ten percent of americans develop a peptic ulcer: one in six of those is infected. four to five million americans have peptic ulcer disease and ulcer disease is responsible for $43 to $44 billion in annual health care costs.	1994	7845945
[celioscopic surgery in duodenal ulcer. the gastroenterologist's point of view].		1994	7847695
perspectives on stomach cancer.		1994	7848574
[colloidal bismuth subcitrate: several pharmacokinetic considerations and of clinical application].		1994	7848694
pharmacokinetic optimisation of the treatment of peptic ulcer in patients with renal failure.	the pathogenesis of peptic ulceration is not yet clear. it could be due to an imbalance between acid secretion and mucosal defensive and/or protective mechanisms, but the association between helicobacter pylori and peptic ulceration has questioned this hypothesis. therefore, drugs inhibiting acid secretion and/or eradicating h. pylori are of major interest. peptic ulcer disease is often associated with renal failure. for the selection of the proper dosage of these agents their pharmacokinetic pr ...	1994	7851056
in vitro activity of cp-99,219, a novel 7-(3-azabicyclo[3.1.0]hexyl) naphthyridone antimicrobial.	the in vitro activity of cp-99,219 was compared with that of ciprofloxacin and sparfloxacin against 814 clinical bacterial isolates using a microdilution method with brain-heart infusion broth. cp-99,219 was the most potent agent tested against methicillin-resistant, ciprofloxacin-susceptible staphylocci (minimum inhibitory concentration [mic]90 < or = 0.25 microgram/ml). cp-99,219 was 32-fold and fourfold more potent than ciprofloxacin and sparfloxacin, respectively, against streptococcus pneum ...	1994	7851087
helicobacter pylori in normal gastric mucosa.	since the discovery of helicobacter pylori (h. pylori) in 1983 several studies have established relationship of h. pylori with gastritis, duodenal ulcer disease and gastric carcinoma. h. pylori infection is widely prevalent and exposure occurs at younger age in our country. several western studies have shown prevalence of h. pylori in normal gastric mucosa to range from 0-25%. as similar information is not available from our country we estimated the prevalence of h. pylori in histologically norm ...	1994	7868485
flagellin gene profiling of helicobacter pylori infecting symptomatic and asymptomatic individuals.	diversity within and around the flagellin (fla) a gene of helicobacter pylori was studied by polymerase chain reaction/restriction fragment length polymorphism (pcr/rflp) analysis and genomic southern blot hybridization profiling. four distinct pattern types were identified by ddei restriction analysis of the 1.5-kb flaa amplicon of 55 strains. most strains (73%) had the same flaa rflp type, but subtypic variation was evident in some strains. no consistent associations were observed for selected ...	1994	7871237
the novel anti-ulcer agent ecabet sodium (ta-2711) eradicates helicobacter pylori colonizing on the gastric mucosa of japanese monkeys.		1994	7871373
positive correlation between h,k-adenosine triphosphatase autoantibodies and helicobacter pylori antibodies in patients with pernicious anemia.	helicobacter pylori is a major cause of gastritis, and the parietal cell h,k-adenosine triphosphatase (atpase) is a major autoantigen in autoimmune atrophic corpus gastritis, which may eventually lead to pernicious anemia and/or neuropathy. whether the bacterium induces the autoimmune response is unknown.	1994	7871374
effects of omeprazole and eradication of helicobacter pylori on gastric and duodenal mucosal enzyme activities and dna in duodenal ulcer patients.	duodenal and gastric content of mucosal enzymes in duodenal ulcer (du) patients differs from that of controls. the purpose of this study has been to examine the effect of omeprazole and eradication of helicobacter pylori on mucosal enzymes in du patients.	1994	7871380
clinically feasible stable isotope technique at a reasonable price: analysis of 13co2/12co2-abundance in breath samples with a new isotope selective-nondispersive infrared spectrometer.	up to now, stable isotope analysis of carbon dioxide in breath samples is carried out with sensitive but very expensive and complex isotope ratio mass spectrometry (irms). aiming at a more widespread application of breath tests in gastroenterological diagnostic routine, we tested a newly developed isotope selective non-dispersive infrared spectrometer (ndirs) in comparison to irms. 13c-urea breath tests were performed in 63 patients as the routine screening method for helicobacter pylori infecti ...	1994	7871857
[low malignancy malt lymphoma of the stomach: h. pyloric eradication as a therapeutic concept?].	a 63 year old patient came to admission because of abdominal pain. a stone disease of the gallbladder was known. gastroscopy showed active gastritis of the antral-mucosa with some erosive lesions. histology revealed surprisingly a low grade malt lymphoma. helicobacter pylori colonization (h. pylori) was found in the mucosa. while additional lymphomas were not found, a therapy aiming at the eradication of h. pylori was started. a subsequent control biopsy showed only slight lymphoplasmacellular i ...	1994	7871859
inhibition of helicobacter pylori urease activity by hydroxamic acid derivatives.	helicobacter pylori (hp) produces strong urease [ec 3.5.1.5], which is considered to play a role in the pathogenesis of gastritis and peptic ulcers. inhibitions against this enzyme have been studied with hydroxamic acid (hxa) derivatives of aliphatic or aromatic carboxylic acids, amino acids and dipeptides. a number of hxas potently inhibited the urease (i50 values were near the order of 10(-6)m), and h-ile-gly-nhoh (i50 = 0.20 x 10(-6)m) was the most potent inhibitor among the derivatives. hp u ...	1994	7874052
[sensitivity of gastric parietal cells to histamine after administration of enprostil and ranitidine during 4 weeks in healthy subjects].	parietal cell sensitivity is increased in patients with active duodenal ulcer. it has been shown to increase in healthy volunteers after a 4-week treatment with h2-receptor blockers. metaanalyses of therapeutic trials have indicated that time to first relapse was longer after ulcer healing with prostaglandins (pg) than after healing with h2-receptor blockers, which might be due to a different effect of the two treatments on parietal cell sensitivity. objectives--to study, in healthy volunteers, ...	1994	7875435
lymphocytic gastritis, gastric adenocarcinoma, and primary gastric lymphoma.	a series of primary gastric lymphomas and adenocarcinomas was reviewed to assess the prevalence of lymphocytic gastritis in these conditions. lymphocytic gastritis was more prevalent in patients with gastric adenocarcinoma (16 of 130 cases; 12.3%) and primary gastric lymphoma (six of 45 cases; 13.7%) than in unselected patients undergoing endoscopy (0.83-2.5%). this suggests that these two disparate gastric tumours may share an immunological dysfunction or a common pathogenesis, and this is of i ...	1994	7876391
gastric heterotopia in the upper esophagus (inlet patch) in endoscopic surveys in northern china.	upper esophageal gastric heterotopia (inlet patch) has not been reported in endoscopic series from china. we describe the occurrence of endoscopically and histologically typical inlet patches in two endoscopic surveys of asymptomatic adults in northern china. because rapid endoscopic protocols were used, the 0.3-0.4% prevalence of inlet patch in these surveys probably represents a minimum figure for adults in this population.	1994	7876515
treatment for helicobacter pylori infection.		1994	7876524
the prevalence of helicobacter pylori positivity in human immunodeficiency virus-infected children.	to investigate the prevalence of helicobacter pylori infection in pediatric patients infected with the human immunodeficiency virus, we sought to detect the presence of antibodies against this organism in 23 human immunodeficiency virus-infected children of central african ethnic origin by means of a second-generation enzyme-linked immunoassay (elisa) test for the detection of immunoglobulin g (igg) antibodies to helicobacter pylori (malakit helicobacter pylori, biolab, limal, belgium). they wer ...	1994	7876996
comparative observations on the types of atrophic gastritis and biopsy pathology of gastric mucosa.	300 cases of chronic atrophic gastritis were classified into 3 types of qi-deficiency of spleen and stomach, yang-deficiency of spleen and stomach, and yin-deficiency of spleen and stomach according to syndrome differentiation of traditional chinese medicine. the biopsy specimens of gastric mucosa were examined histopathologically and for the presence of pyloric spirobacteria. the results showed that the type of yin-deficiency was the most serious in the degree of pathologic changes, or the glan ...	1994	7877344
[helicobacter pylori in patients with gastroduodenal pathology at a young age].		1994	7879558
helicobacter pylori infection in children with recurrent abdominal pain.	forty-eight patients with recurrent abdominal pain were studied endoscopically for the presence of gastritis and helicobacter pylori infection. fifteen of these children had histological evidence of gastritis with eight of them having concomitant h. pylori infection. the majority of those with h. pylori infection had severe degrees of acute following chronic gastritis.	1994	7880086
how helicobacter pylori acquired its name, and how it overcomes gastric defence mechanisms.	at royal perth hospital, western australia in april 1982, spiral bacteria were first cultured from a gastric biopsy specimen. several important taxonomic features were identified which indicated that these bacteria represented a new genus. in october 1989 the new genus name helicobacter was published. helicobacter pylori overcomes gastric defence mechanisms by means of its powerful urease enzyme, by its spiral shape allowing it to penetrate mucus, by adherence to the gastric mucosa and by variou ...	1994	7881010
gastric mucosal defensive factors: the therapeutic strategy.	there are several interesting approaches to augmenting defence or repair mechanisms that can be used already or may find a place in therapy for ulcer disease. factors such as epidermal growth factor and basic fibroblast growth factor show potential. alternative strategies might be to stimulate mucosal blood flow with agents that release nitric oxide (no), or to scavenge free radicals in the inflamed or ischaemic mucosa. if such approaches are to find a role in therapy, it is likely that it will ...	1994	7881011
the therapeutic strategy for peptic ulcer disease.	therapy of acid/peptic disease has evolved since the 1970s with development of: (i) more accurate endoscopes which permit precise examination and documentation of upper gastrointestinal lesions; and (ii) the histamine h2-receptor antagonists. as well, refined standards for clinical investigation have contributed to the clinical study of acid/peptide diseases. initially, ulcer diseases were considered to be principally secondary to increased 'aggressive' factors (acid, pepsin) and the therapeutic ...	1994	7881012
effect of ammonium ion on the hydrophobic and barrier properties of the gastric mucus gel layer: implications on the role of ammonium in h. pylori-induced gastritis.	infection with the bacterium helicobacter pylori is associated with both the development of gastritis and an attenuation in the hydrophobic properties of the stomach. in order to better understand the effect of ammonium, one of the major products of h. pylori urease on these properties, a series of in vivo and in vitro experiments was performed. in the in vivo studies rats were intragastrically administered nh4cl alone and in combination with the mucolytic agent, muco-mist, in various dosing str ...	1994	7881013
helicobacter pylori epidemiology in relation to peptic ulcer and gastric cancer in south and north china.	peptic ulcer disease is twice as common in people living in south china when compared with those in north china. however, the gastric cancer rate in north china is three times that of south china. the overall helicobacter pylori prevalence rate is similar in north and south china populations and it is higher than caucasians living in western societies. however, there is a significantly higher prevalence of h. pylori seropositivity among those who live in the high gastric cancer areas of china. o ...	1994	7881017
how does helicobacter pylori cause duodenal ulcer disease: the bug, the host, or both?	helicobacter pylori infection is the most common cause of duodenal ulcer disease, yet duodenal ulcer is an uncommon outcome of h. pylori infection. we reviewed the possible explanations such as differences in the host or in the strain of h. pylori. host factors reviewed included genetic susceptibility to h. pylori infection and excess gastric acid secretion. the role of potential h. pylori virulence factors not present in all strains such as the caga gene and the results of other molecular metho ...	1994	7881025
aetiological factors of peptic ulcer: perspectives of epidemiological observations this century.	there are five known environmental, causative factors for peptic ulcer, namely, non-steroidal anti-inflammatory drugs (nsaid), helicobacter pylori infection, cigarette smoking, environmental stress and dietary habit. there have been six factual, epidemiological observations on peptic ulcer this century: the rise and fall of ulcer frequency in western societies; geographical variations in ulcer rates; in sex ratios; in duodenal: gastric ulcer ratios; and in placebo healing rates; and seasonal var ...	1994	7881028
clinical relevance of basic research in peptic ulcer disease.	historically, the interplay between basic research and clinical observation has been essential in the development of new therapies for peptic ulcer disease. that histamine is an important regulator of acid secretion emerged from basic research, followed by the clinical development and use of the h2-receptor antagonists. basic research contributed again by defining the importance of h+/k(+)-atpase in acid secretion, resulting in a new class of useful antisecretory agents. basic studies also gave ...	1994	7881029
[helicobacter pylori in the dental plaque].	helicobacter pylori (hp) were detected in the dental plaque in 40 patients with peptic ulcer, chronic gastritis or gastric cancer with rapid urease test, anti-hp fluorescein-labelled antibody staining, bacterial culture and electronic microscopy. at the same time, biopsy specimens from the gastric antrum of these patients were studied with ws staining and rapid urease test to detect hp. the results show that a great lot of hps is present in most of the patients' dental plaque. the morphological, ...	1994	7867441
[healing quality and recurrence of ulcer diseases].		1994	7882737
helicobacter pylori in dental plaque of children and their family members.	a prospective study was undertaken to determine the presence of helicobacter pylori in the dental plaque of children and their family members. 22 children (age range: 2-12 years; males: 16) admitted to the paediatric ward for various disorders and 17 healthy family members (age range: 7-40 years; males: 9) of 13 of these children were screened for presence of helicobacter pylori in the dental plaque by the rapid urease test. h. pylori was detected in dental plaque of 82% (18/22) children and 88% ...	1994	7883668
helicobacter sp. strain mainz isolated from an aids patient with septic arthritis: case report and nonradioactive analysis of 16s rrna sequence.	a campylobacter-like organism was isolated from an effusion of the left knee joint of an aids patient 2 weeks after bacteremia with a morphologically identical organism. amplified genomic 16s rrna sequences were analyzed by a nonradioactive blotting technique. the closest match was found with helicobacter fenelliae (97.7% homology). sequence data and phenotype suggest that the isolate may represent a so far unrecognized species of the genus helicobacter.	1994	7883895
transportation of helicobacter pylori cultures by optimal systems.	cultures of helicobacter pylori on chocolate agar slants in bijou bottles and on chocolate agar plates inside bbl campy pouches were mailed from dublin to galway, ireland; bordeaux, france; and beijing, china. both systems maintained viability of h. pylori for at least 4 days under mailing conditions. ninety percent of the isolates on the slants survived for 6 days, but only 30% of the isolates in the pouches survived. when the slants were stored at 4 degrees c after arrival, 50% of the isolates ...	1994	7883907
micronutrients and cancer aetiology: the epidemiological evidence.	micronutrient deficiencies occur most commonly in poor countries and, therefore, are most likely to be associated with cancers common in these countries. epidemiological studies are hampered by inaccurate measurement of micronutrient intake and by the correlations between intakes of many nutrients. the strongest evidence for a protective effect of micronutrients is for oesophageal cancer. the identity of the micronutrients is not certain, but may include retinol, riboflavin, ascorbic acid and zn ...	1994	7886059
helicobacter heilmannii (formerly gastrospirillum hominis) gastritis: an infection transmitted by animals?	the source of infection with helicobacter heilmannii (formerly gastrospirillum hominis), a relatively rare causative agent of gastritis in humans, is not clear. it has long been known that this organism occurs in the stomach of domestic animals and pets.	1994	7886392
effects of omeprazole and lansoprazole on 24-hour intragastric ph in helicobacter pylori-positive volunteers.	the comparative effects of omeprazole and lansoprazole on gastric acidity in helicobacter pylori-positive subjects and the clearance of h. pylori are unknown.	1994	7886393
omeprazole plus amoxicillin for cure of helicobacter pylori infection. factors influencing the treatment success.	omeprazole plus amoxicillin may cure helicobacter pylori infection. however, the published results vary rather widely, and the factors influencing the treatment success remain unclear.	1994	7886394
use of a receiver operating characteristic in the evaluation of two commercial enzyme immunoassays for detection of helicobacter pylori infection.	two novel commercial igg enzyme immunoassay (eia) systems based on acid-glycine-extracted (pyloriset igg eia, orion diagnostica) or fast protein liquid chromatography-purified (cobas core anti-h. pylori eia, roche diagnostic systems) helicobacter antigens were evaluated in a prospective study involving 127 patients. all patients underwent upper endoscopy with biopsy, and biopsies were examined for the presence of helicobacter pylori by a rapid urease test, microscopy and culture. of the 71 patie ...	1994	7889950
luxuriant growth of helicobacter pylori and campylobacter species in candle jars after primary isolation.		1994	7889954
bacterioferritin: a hemoprotein member of the ferritin family.		1994	7887220
ranitidine bismuth citrate and ranitidine do not affect gastric emptying of a radio-labelled liquid meal.	ranitidine bismuth citrate, a new chemical entity which is a salt complex of ranitidine and bismuth citrate, is being developed for the treatment of relapse of benign gastric and duodenal ulcer and eradication of helicobacter pylori. the aim of the present study was to establish whether ranitidine bismuth citrate (800 mg) or ranitidine hydrochloride (300 mg) have any effect on gastric emptying of a liquid meal using gamma scintigraphy. on three separate occasions, each of twelve subjects receive ...	1994	7888296
non-motile mutants of helicobacter pylori and helicobacter mustelae defective in flagellar hook production.	flagellar hooks were purified from helicobacter pylori and helicobacter mustelae. the 70 x 16 nm h. pylori hook was composed of flge subunits of 78kda, while the 72 x 16 nm h. mustelae hook was composed of 87 kda subunits. n-terminal sequence was obtained for the flge proteins of both species, and for an internal h. mustelae flge peptide. degenerate oligonucleotide primers allowed amplification of a 1.2 kb fragment from the h. mustelae chromosome, which carried part of the flge gene. the corresp ...	1994	7891557
[prognostic significance of helicobacter pylori isolation in patients with peptic ulcer].	the author shows that persistent contamination of the mucosa in ulcer patients noticeable even after ulcer healing with helicobacter pylori leads to disordered coordination of energetic processes in immunocompetent cells. the reserve pathway becomes more active than basic pathway of oxidation, this combining with lymphocyte sensitization to the mucosal tissue. an unfavourable prognostic implication is given to a 1.5-fold inhibition of lymphocyte succinate dehydrogenase activity. if alpha-glycero ...	1994	7898008
coccoid helicobacter pylori not culturable in vitro reverts in mice.	an experimental rodent model was used to demonstrate the viability of the coccoid form of helicobacter pylori. concentrated suspensions were prepared for the two different morphologies: at 2 days incubation for the bacillary forms and at 20 days incubation for the "dormant" forms. the strains used for incubation were two fresh isolates from humans with duodenal ulceration, and two collection strains. five hundred microliters of culture (od550 = 5 mc farland) of helicobacter pylori with bacillary ...	1994	7898382
growth medium containing cyclodextrin and low concentration of horse serum for cultivation of helicobacter pylori.	the growth of helicobacter pylori, a gram-negative microaerophilic bacterium, is often difficult and requires complex media with the supplementation of 5% to 10% blood or blood derivatives. we have found that brucella broth supplemented with 1% heated horse serum and 0.1% beta-cyclodextrin supports the good growth of h. pylori. the degree of growth and production of urease and vacuolating cytotoxin in this medium were equal to those in the medium supplemented with 5% horse serum. this medium was ...	1994	7898389
[the omeprazole treatment of patients with diseases of the upper digestive tract].	effectiveness of the use of omeprazolum (oside) was studied in 24 patients with ulcer disease, 16 patients with chronic primary gastroduodenitis and 18 patients with acid reflux-esophagitis. the preparation oside is a highly effective agent for the treatment of the aforementioned conditions and possesses a powerful antisecretory and antioxidant activity. against the background of the use of the preparation the count of helicobacter pylori gets decreased in the antral part of the stomach. the use ...	1994	7900331
[the pathogenesis of peptic ulcer].		1994	7900339
[defence system of gastroduodenal mucous glands in ulcer disease].	as a result of complex investigation of the dafence mucosal barrier of gastroduodenal zone (studying of glicoproteins and immunoglobulins secretion), the acid-producing stomach function and its colonization with helicobacter pylori bacteria it was determined that side by side with infringement of polysaccharide-albuminous complexes secretion the weakening of humoral and nonspecific local immunity occurs. the infringement degree depends on severity of ulcer disease. on the background of autoimmun ...	1994	7707641
detection of helicobacter pylori in endoscopic biopsies in sudan.	we studied the prevalence of helicobacter pylori in sudanese subjects with gastroduodenal inflammation. h. pylori was looked for in biopsy specimens taken from the antrum by two methods: rapid urease test [campylobacter-like organism (clo) test] and culture using skirrow's selective supplement. one hundred subjects were studied. h. pylori was found in 80% of patients with gastritis, 56% of patients with duodenal ulcer, 60% of patients with duodenitis and 16% of normal control subjects. it was ne ...	1994	7801358
unravelling the pathogenic role of helicobacter pylori in peptic ulcer: potential new therapies and vaccines.	the recognition that peptic ulcer is an infectious disease caused by the bacterium helicobacter pylori has revolutionized the approach to diagnosis and therapy of this condition. treatment of the symptoms of peptic ulcer with drugs that block acid secretion is already being replaced by antibiotic eradication of the causative agent. studies of the molecular events that lead to h. pylori pathogenesis have shown that clinical isolates can be divided into two groups, only one of which produces a cyt ...	1994	7765388
helicobacter pylori and chronic gastritis: anatomico-functional correlations.		1994	7812032
association of alcohol consumption and helicobacter pylori infection in young adulthood and early middle age among patients with gastric complaints. a case-control study on finnish conscripts, officers and other military personnel.	there is growing evidence that helicobacter pylori is responsible for a variety of gastric and duodenal changes which can eventually lead to stomach cancer. little is known about risk factors for h. pylori infection. we re-analyzed the association of alcohol with h. pylori positivity in 451 conscripts, officers and other military personnel endoscoped due to gastric complaints in the central military hospital of finland in 1987 and 1988. serology and culture were done in all patients. alcohol con ...	1994	7813699
helicobacter pylori infection and gastritis in healthy nigerians.	in a study of forty asymptomatic volunteers from northern nigeria; 35 (87.5%) had histological gastritis and 32 (80%) were infected by helicobacter pylori. all but one of the patients infected by helicobacter pylori had histological gastritis. this high prevalence of h. pylori infection in young, asymptomatic subjects, occurs in an area with a low prevalence of duodenal ulcer. the possible reasons for this are discussed.	1994	7813703
[gastric lymphoma--an infectious disease?].		1994	7813934
prospective evaluation of upper gastrointestinal mucosal lesions in children with ulcerative colitis and crohn's disease.	eighty-eight consecutive children with inflammatory bowel disease were studied, and upper gastrointestinal endoscopy was performed in 80 of them as one of the initial investigations before commencing medical or nutritional treatment. forty-one children were found to have crohn's disease and 47, ulcerative colitis. upper gastrointestinal endoscopy revealed pathology in 32 (80%) cases of crohn's disease, esophagitis in 16, and esophageal ulcer in two, nonspecific gastritis in 22, duodenitis or duo ...	1994	7815240
marked gastric mucosal hyperplasia without definite peptic ulceration related to a multifocal hepatic gastrinoma.		1994	7815264
is interstitial cystitis an infectious disease?	interstitial cystitis (ic) is manifest by years of urinary frequency, urgency, and bladder pain and on cystoscopy, is diagnosed by petechial hemorrhages or ulcers. the etiology is unknown; the prominent theories are that ic is an autoimmune disease or is linked to increased permeability of the bladder mucosa. although sought, no infectious agent has ever been identified. the disease has many characteristics of a chronic infection and the author's opinion is that an infectious disease has not bee ...	1994	7815976
helicobacter pylori in patients undergoing upper endoscopy in jamaica.	one hundred and two consecutive patients undergoing upper gastrointestinal endoscopy were tested for h. pylori by a rapid urease test, using antral biopsy specimens. there were 60 men (mean age 54 yrs) and 42 women (mean age 49 yrs). fifty-six patients (55%) were positive for h. pylori. of male patients, 36 (60%) and of female patients, 20 (48%) tested positive. sixty-eight per cent of patients with antral gastritis, 65% with duodenal ulcer and 60% with gastric ulcer had h. pylori. thirty-nine p ...	1994	7817542
helicobacter pylori in patients with systemic sclerosis: detection with the 13c-urea breath test and eradication.	in patients with systemic sclerosis peristaltic abnormalities may delay gastric emptying, giving rise to bacterial overgrowth, including possibly helicobacter pylori (hp). infection with helicobacter is an important risk factor for esophageal and gastric diseases, including esophagitis, gastritis and gastric cancer. the purpose of this prospective study was to assess gastric hp infection in patients with systemic sclerosis. in 12 patients with systemic sclerosis the newly introduced breath test ...	1994	7817672
serum gastrin, pepsinogens, parietal cell and helicobacter pylori antibodies in patients with gastric polyps.	to determine serum concentration of gastrin and pepsinogens (pgs) as markers for the gastric mucosal status and to elucidate the prevalence of serum helicobacter pylori (h pylori) igg antibodies and parietal cell autoantibodies (pcas) in patients with gastric polyps.	1994	7817697
[chronic gastritis associated with helicobacter pylori in children].		1994	7818732
[new types of chronic gastritis].		1994	7818733
eradication of helicobacter pylori should be pivotal in managing peptic ulceration. eradication largely prevents relapse.		1994	7819903
eradication of helicobacter pylori should be pivotal in managing peptic ulceration. helicobacter pylori is not the causative agent.		1994	7819904
helicobacter pylori: the new bug on the (paraffin) block.	a nameless spirillar organism in search of a disease only a few years ago, helicobacter pylori has seen its fortunes suddenly reversed. after a rapid succession of name changes and some acrimonious disputes across continents, in less than a decade h. pylori has been catapulted to the centre stage of gastroenterological and microbiological research and has topped the most-wanted list of the pharmaceutical industry. the discovery of h. pylori has provided the momentum for the formation of the grou ...	1994	7820298
helicobacter pylori: after ten years, still an enigma.		1994	7820889
possible role of helicobacter pylori serology in reducing endoscopy workload.	we validated a commercial enzyme-linked immunosorbent assay (elisa), helico-g, in diagnosing h. pylori in 129 patients (mean age 50 years, range 15-86). we analysed the results of endoscopy against serology to see whether there was a possibility of adopting the strategy of not endoscoping dyspeptic subjects under the age of 45. h. pylori infection was considered present if either histology and/or culture were positive. the elisa had a sensitivity of 88%, specificity of 72%, positive predictive v ...	1994	7824415
helicobacter pylori infection and fasting serum gastrin levels in a series of endoscopically diagnosed gastric polyps.	the occurrence of h. pylori infection and the levels of fasting serum gastrin (sega) were examined in 97 patients with different morphological types of endoscopically diagnosed gastric polyps. according to the histology of the polyps the series was divided into three groups: inflammatory polyps (43 cases), polyps with foveolar hyperplasia (25 cases), and hyperplastic polyps including adenomas (29 cases). the prevalence of h. pylori infection was significantly lower in patients with hyperplastic ...	1994	7826605
prevalence of helicobacter pylori in southern indian controls and patients with gastroduodenal disease.	the spiral organism helicobacter pylori has been causally implicated in the genesis of various gastroduodenal diseases. since these diseases are common in southern india, this study was undertaken to determine the prevalence of h. pylori in the gastric mucosa of asymptomatic adults and patients with various gastroduodenal diseases. h. pylori was detected in the gastric mucosa of 25 of 30 (83.3%) normal volunteers. prevalence rates in the disease groups were also high, and included 38 of 41 patie ...	1994	7827302
colonization by helicobacter pylori and its relationship to histological changes in the gastric mucosa in portal hypertension.	in order to investigate the relationship between helicobacter pylori infection of the gastric mucosa and mucosal changes in portal hypertension, gastric fundic and antral biopsies were obtained from 66 patients with portal hypertension and 49 controls with non-ulcer dyspepsia (nud). gastric mucosa from portal hypertensive patients exhibited typical vascular dilatation and congestion, while mild dilatation of lamina propria blood vessels was not uncommon in nud patients with histological evidence ...	1994	7827303
gastric cytoprotection by amoxycillin in the rat.	amoxycillin in combination with anti-ulcer agents has been shown to prolong duodenal ulcer remission. while this effect can be related to the eradication of helicobacter pylori, it is not known if amoxycillin might possess cytoprotective properties. protection against ethanol-induced gastric mucosal damage by intragastric instillation of amoxycillin suspension (prepared from capsule form) and solution (from injection form, 100% pure amoxycillin), and by intraperitoneal injection of amoxycillin s ...	1994	7827304
carcinogenicity of some biological agents.		1994	7827595
inhibitory effect of polyunsaturated fatty acids on the growth of helicobacter pylori: a possible explanation of the effect of diet on peptic ulceration.	diets high in polyunsaturated fatty acids may protect against duodenal ulcer, possibly through inhibiting the growth of helicobacter pylori. this hypothesis was tested in vitro by incubating h pylori microaerophilically with a range of polyunsaturated fatty acids. omega-3 linolenic acid significantly, but reversibly, inhibited growth at 1.8, 2.5, and 5 x 10(-4) m (p < 0.01), while concentrations of 10(-3) m killed virtually all organisms, with cell lysis observed by electron microscopy. similar ...	1994	7828972
high prevalence of helicobacter pylori metronidazole resistance in migrants to east london: relation with previous nitroimidazole exposure and gastroduodenal disease.	a high prevalence of metronidazole resistance in helicobacter pylori is reported in developing countries. this study examined whether migrants referred for diagnostic gastroscopy at a united kingdom centre (n = 54), had a higher prevalence of metronidazole resistance than subjects born in the united kingdom attending endoscopy (n = 46). records of nitroimidazole treatment prescribed in the united kingdom was obtained in 83 patients to find out if there was an association between h pylori metroni ...	1994	7828973
cytokine gene expression in helicobacter pylori associated antral gastritis.	infection of the gastric antrum by helicobacter pylori is characterised by a cellular inflammatory infiltrate. whether cytokines are involved in the pathogenesis of this gastritis has been investigated by studying the effect of eradicating h pylori on the expression of genes encoding the cytokines interleukin 8 (il-8) and tumour necrosis factor alpha (tnf-alpha) in the antral mucosa. gastric antral biopsy specimens were taken from nine patients with duodenal ulcers and cytokine transcripts were ...	1994	7828974
improved fold width and increased acid secretion after eradication of the organism in helicobacter pylori associated enlarged fold gastritis.	this study examined the effects of eradication of helicobacter pylori (h pylori) infection on gastric mucosal morphology and acid secretion. sixteen h pylori positive patients with enlarged gastric body folds were divided into two groups: (a) patients with moderate enlargement (fold width: 6 to 10 mm, n = 8) and (b) patients with severe enlargement (> 10 mm, n = 8). after successful treatment, gastric body fold width was reduced in both groups (p < 0.01) with an associated decrease in inflammato ...	1994	7828975
helicobacter pylori in eastern european countries: what is the current status?		1994	7829002
the susceptibility of helicobacter pylori to 12 antimicrobial agents, omeprazole and bismuth salts.		1994	7829415
helicobacter pylori eradication: who, why and how in 1994?		1994	7830661