Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| specific humoral reaction of hemorrhagic fever with renal syndrome (hfrs) patients in china to recombinant nucleocapsid proteins from european hantaviruses. | hemorrhagic fever with renal syndrome (hfrs) is endemic in east asia and europe. this study was initiated to investigate the reactivity of antibodies in sera of chinese hfrs patients with the recombinant nucleocapsid proteins (rnps) of hantaan virus (htnv), dobrava-belgrade virus (dobv), and puumala virus (puuv), which are the prevalent hantavirus strains in europe. forty-eight pairs of acute and convalescent sera were collected from hfrs patients in hubei, china (1985-2002) and tested by indire ... | 2011 | 21222012 |
| time to revise the paradigm of hantavirus syndromes? hantavirus pulmonary syndrome caused by european hantavirus. | hantaviruses have previously been recognised to cause two separate syndromes: hemorrhagic fever with renal syndrome in eurasia, and hantavirus pulmonary syndrome (hps) in the americas. however, increasing evidence suggests that this dichotomy is no longer fruitful when recognising human hantavirus disease and understanding the pathogenesis. herein are presented three cases of severe european puumala hantavirus infection that meet the hps case definition. the clinical and pathological findings we ... | 2011 | 21234633 |
| hantavirus pulmonary syndrome: encephalitis caused by virus andes. | hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome (hps) are rodent-borne emerging diseases caused by members of the genus hantavirus, family bunyaviridae. some species of hantavirus may cause encephalitis, but this is the first report in andes virus associated to hps. | 2011 | 21240581 |
| seroepidemiological survey of hantavirus infection in healthy people in vallès occidental, barcelona. | hantaviruses are the etiological agents of hemorrhagic fever with renal syndrome in europe and asia, and hantavirus pulmonary syndrome in america. approximately 150,000 cases are reported annually worldwide. in spain, some hantavirus infection cases have been described. besides, rodents that have been described as hantavirus reservoirs are present. the aim of the present study was to determinate the seroprevalence of hantavirus in humans in the northeast of spain. | 2011 | 21417923 |
| interview with pedro fernando da costa vasconcelos, md, phd. interview by vicki glaser. | 2011 | 21261512 | |
| serological evidence of thailand virus-related hantavirus infection among suspected leptospirosis patients in kandy, sri lanka. | a cross-sectional study was undertaken to determine the current prevalence of leptospirosis and hantaviral infections, and the socio-demographic characteristics and risk factors of infected patients, in kandy, sri lanka. this report discusses the serological evidence of hantavirus infections among 105 suspected leptospirosis patients, 8 of whom had hantavirus antibodies. serotyping elisa showed that these 8 patients had high optical density values for thailand virus. most of the sera showed that ... | 2011 | 21266762 |
| prevalence of hantavirus infection in wild rodents from five provinces in korea, 2007. | hemorrhagic fever with renal syndrome (hfrs) is an infectious disease caused by hantaviruses of the family bunyaviridae. among them, hantaan virus (htnv) is most widely distributed in korea. the striped field mouse, apodemus agrarius, is the natural host of htnv in rural korea. we trapped 766 small mammals of three species (1 eothenomys regulus, 13 crocidura suaveolens, and 752 apodemus agrarius) in five provinces in korea from january to december 2007. we tested 542 rodent sera for htnv antibod ... | 2011 | 21441196 |
| genetic evidence of hantavirus infections in wild rodents from northwestern colombia. | this report builds on recent serological evidence for the presence of hantavirus in northern colombia by providing sequence-specific and phylogenetic data of hantavirus infections in wild rodents. from august 2007 to august 2008, 354 rodent specimens representing four families were collected in the northwestern antioquia region of colombia. antibodies reactive to sin nombre virus and maciel virus antigens by igg enzyme-linked immunosorbent assay were found in 15 of 109 (14%) cherries cane rats ( ... | 2011 | 21284514 |
| hantavirus ecology in rodent populations in three protected areas of argentina. | in this study, we identified hantavirus genotypes and their reservoirs and evaluated the spatial and temporal distribution of the virus in rodent population in three protected areas of argentina over 3ôçâyears (2007-2010). a total of 837 rodents were captured with an effort of 22ôçâ117 trap-nights. we detected the genotype lechiguanas in oligoryzomys nigripes and o.-áflavescens and pergamino in akodon azarae. there was no correlation between seroprevalence and trap success of the host. the propo ... | 2011 | 21733047 |
| infection of hantaan virus strain aa57 leading to pulmonary disease in laboratory mice. | hantaan virus (htnv) is a causative agent of hemorrhagic fever with renal syndrome (hfrs). the pathogenesis of hfrs has not been fully elucidated, mainly due to the lack of a suitable animal model. in laboratory mice, htnv causes encephalitis. however, that symptom is dissimilar to human hantavirus infections. we found that htnv strain aa57 (isolated from apodemus agrarius in far east russia) caused pulmonary disease in 2-week-old icr mice. the clinical signs of the infected mice were piloerecti ... | 2011 | 22044619 |
| subarachnoid hemorrhage associated with epidemic hemorrhagic fever: a rare case report. | nervous system injuries associated with epidemic hemorrhagic fever (ehf) are not rarely seen. however, cerebrovascular disease arising from ehf is rarely reported in the literature. a 50-year-old male patient suffered from subarachnoid hemorrhage (sah). no abnormal condition was found in intracranial vascular digital subtraction angiography (dsa). but, this patient presented with positive hantavirus-igm and igg, with typical clinical process, which lead to the diagnosis of ehf followed by sah. t ... | 2011 | 22135608 |
| [acute myopia]. | this paper presents a case of ocular involvement during acute renal failure caused by hantavirus. a 34-year-old man suffered from sudden visual loss during acute onset fever with nausea and renal failure. the ophthalmologic examination showed myopia in both eyes and no signs of infection but virological studies revealed an infection with hantavirus. during treatment of the renal failure the myopia improved spontaneously. transient myopia is a typical ocular involvement during infections with han ... | 2011 | 21688053 |
| young male patients are at elevated risk of developing serious central nervous system complications during acute puumala hantavirus infection. | abstract: | 2011 | 21838931 |
| notes from the field: hantavirus pulmonary syndrome --- maine, april 2011. | on april 25, 2011, the maine center for disease control and prevention was notified of a suspected case of hantavirus pulmonary syndrome (hps) in a man aged 70 years with no recent out-of-state travel. the maine resident went to a community hospital in early april with a 5-day history of fatigue, decreased appetite, weakness, chills, myalgias, and progressive shortness of breath. on examination, he was hypoxic and tachypneic. the patient was admitted with laboratory evidence of acute renal insuf ... | 2011 | 21681176 |
| extracorporeal membrane oxygenation support improves survival of patients with hantavirus cardiopulmonary syndrome refractory to medical treatment. | the aim of the study was to evaluate the outcome of extracorporeal membrane oxygenation (ecmo) support in hantavirus cardiopulmonary syndrome (hcps) patients with a predicted mortality of 100%, and the complications associated with this treatment modality and different cannulation techniques. | 2011 | 21900022 |
| comparison of innate immune responses to pathogenic and putative non-pathogenic hantaviruses in vitro. | hantaviruses are human pathogens that cause hemorrhagic fever with renal syndrome or hantavirus cardiopulmonary syndrome. the mechanisms accounting for the differences in virulence between pathogenic and non-pathogenic hantaviruses are not well known. we have examined the pathogenesis of different hantavirus groups by comparing the innate immune responses induced in the host cell following infection by pathogenic (sin nombre, hantaan, and seoul virus) and putative non-pathogenic (prospect hill, ... | 2011 | 21820021 |
| The Andes Hantavirus NSs protein is expressed from the viral Small mRNA by a leaky scanning mechanism. | The small messenger RNA (SmRNA) of all Bunyaviridae encodes the nucleocapsid (N) protein. In 4 out of 5 genera in the Bunyaviridae the SmRNA encodes an additional non-structural protein denominated NSs. In this study we show that Andes hantavirus (ANDV) SmRNA encodes a NSs protein. Data show that the NSs protein is expressed in the context of an ANDV infection. Additionally, our results suggest that translation initiation from the NSs-initiation codon is mediated by ribosomal subunits that have ... | 2011 | 22156529 |
| pathogenic old world hantaviruses infect renal glomerular and tubular cells and induce disassembling of cell-to-cell contacts. | viral hemorrhagic fevers are characterized by enhanced permeability. one of the most affected target organs of hantavirus-induced hemorrhagic fever with renal syndrome is the kidney, and an infection often results in acute renal failure. to study the underlying cellular effects leading to kidney dysfunction, we infected human renal cell types in vitro that are critical for the barrier functions of the kidney and examined kidney biopsies of hantavirus-infected patients. we analyzed the infection ... | 2011 | 21775443 |
| [the expression and bioactivity of s gene in vero-e6 cells using baculovirus vector]. | the s gene of a hanta virus (hv) z10 strain was cloned into a baculovirus shuttle bacmid pdual-cmv contained a cmv promoter to generated a recombinant baculovirus bac-pdual-cmv-hvs, then the recombinant baculovirus was transfected into vero-e6 cell. the cells with recombinant baculovirus were applied to the detection of hv antiserum. | 2011 | 22097607 |
| antigenic characterization of expressed complete and different truncated recombinant nucleocapsid proteins of hantaan virus by monoclonal antibodies. | the nucleocapsid protein (np) of hantavirus has been shown to be highly immunogenic in laboratory animals and humans, and it induces an early and long-lasting humoral immune response during hantavirus infection. in the present study, entire and three partially truncated open reading frames (orf) of np genes of hantaan virus (htnv) strain 76-118, a prototype of hantavirus, were amplified and then cloned in prokaryotic expression vectors. the recombinant whole np and truncated nps were expressed i ... | 2011 | 22008071 |
| t cells are not required for pathogenesis in the syrian hamster model of hantavirus pulmonary syndrome. | andes virus (andv) is associated with a lethal vascular leak syndrome in humans termed hantavirus pulmonary syndrome (hps). in hamsters, andv causes a respiratory distress syndrome closely resembling human hps. the mechanism for the massive vascular leakage associated with hps is poorly understood; however, t cell immunopathology has been implicated based on circumstantial and corollary evidence. here, we show that following andv challenge, hamster t cell activation corresponds with the onset of ... | 2011 | 21775442 |
| application of truncated nucleocapsid protein (n) for serotyping elisa of murinae-associated hantavirus infection in rats. | truncated recombinant nucleocapsid proteins (trns) that lack n-terminally located cross-reactive epitopes of four murinae rodent-associated hantaviruses, seoul virus (seov), thailand virus, hantaan virus (htnv) and dobrava-berglade virus, were produced by using a baculovirus expression system. elisa with the trns as antigens enabled serotyping of immune sera from rats experimentally inoculated with the corresponding hantaviruses as cut-off od values of 60% to those to whole n of htnv. the trn ba ... | 2011 | 21937858 |
| an unusual cause of a usual presentation. hantavirus infection. | 2011 | 21868815 | |
| phylogenetic exploration of hantaviruses in paraguay reveals reassortment and host switching in south america. | abstract: | 2011 | 21838900 |
| a model system for in vitro studies of bank vole borne viruses. | the bank vole (myodes glareolus) is a common small mammal in europe and a natural host for several important emerging zoonotic viruses, e.g. puumala hantavirus (puuv) that causes hemorrhagic fever with renal syndrome (hfrs). hantaviruses are known to interfere with several signaling pathways in infected human cells, and hfrs is considered an immune-mediated disease. there is no in vitro-model available for infectious experiments in bank vole cells, nor tools for analyses of bank vole immune acti ... | 2011 | 22194969 |
| Detection of two morphologically cryptic species from the cursor complex (Akodon spp; Rodentia, Cricetidae) through the use of RAPD markers. | The cursor complex is a group within the Akodon genus of South American rodents, formed by Akodon cursor and A. montensis. Correct distinction between these two species is of great importance since they can harbor different Hantavirus strains. These species are only distinguishable by means of karyotypic or internal anatomic features, requiring dissection; recently, some other genetic methods have become available. We developed RAPD markers capable of distinguishing between A. cursor and A ... | 2011 | 22179960 |
| the delicate pygmy rice rat (oligoryzomys delicatus) is the principal host of maporal virus (family bunyaviridae, genus hantavirus). | abstract choclo virus (chov) and maporal virus (mapv) are enzootic in panama and western venezuela, respectively. the results of previous studies suggested that the fulvous pygmy rice rat (oligoryzomys fulvescens) is the principal host of both viruses. the results of an analysis of nucleotide sequence data in this study indicated that the rodent associated with chov is the costa rican pygmy rice rat (oligoryzomys costaricensis) and that the rodent associated with mapv is the delicate pygmy rice ... | 2011 | 21548760 |
| maporal virus as a surrogate for pathogenic new world hantaviruses and its inhibition by favipiravir. | pathogenic hantaviruses geographically distributed in the old world cause haemorrhagic fever with renal syndrome (hfrs), whereas new world hantaviruses are the aetiological agents of hantavirus cardiopulmonary syndrome (hcps). ribavirin, a drug associated with toxicities, is presently indicated for treatment of hfrs, whereas treatment of the more frequently lethal hcps is limited to supportive care. because of the need for safe and effective antivirals to treat severe hantaviral infections, we e ... | 2011 | 21566265 |
| serological evidence of hantavirus infection in farm and abattoir workers in trinidad--a preliminary study. | hantaviruses are established causative agents of hemorrhagic fevers and renal diseases amongst other clinical manifestations in humans, with most diagnosis based on serological assays. the disease, which is rodent-borne, has been reported in numerous countries worldwide but information about the disease is scanty in the caribbean. the objective of this investigation is to determine the frequency of exposure to hantaviruses in a selected apparently healthy human population associated with abattoi ... | 2011 | 21728870 |
| expression of recombinant araraquara hantavirus nucleoprotein in insect cells and its use as an antigen for immunodetection compared to the same antigen expressed in escherichia coli. | abstract: background: antigens for hantavirus serological tests have been produced using dna recombinant technology for more than twenty years. several different strategies have been used for that purpose. all of them avoid the risks and difficulties involved in multiplying hantavirus in the laboratory. in brazil, the araraquara virus is one of the main causes of hantavirus cardio-pulmonary syndrome (hcps). methods. in this investigation, we report the expression of the n protein of the araraqua ... | 2011 | 21569341 |
| phylogenetic analysis of puumala virus subtype bavaria, characterization and diagnostic use of its recombinant nucleocapsid protein. | puumala virus (puuv) is the predominant hantavirus species in germany causing large numbers of mild to moderate cases of haemorrhagic fever with renal syndrome (hfrs). during an outbreak in south-east germany in 2004 a novel puuv subtype designated bavaria was identified as the causative agent of hfrs in humans [1]. here we present a molecular characterization of this puuv strain by investigating novel partial and almost entire nucleocapsid (n) protein-encoding small (s-) segment sequences and p ... | 2011 | 21598005 |
| sex differences in the incidence and case fatality rates from hemorrhagic fever with renal syndrome in china, 2004-2008. | background. differences between male and female individuals in response to infectious diseases are an overlooked global health problem. methods. the relationship between sex and disease outcome was examined in populations of patients with hemorrhagic fever with renal syndrome (hfrs) in mainland china, where most cases of hantavirus exposure occur. hfrs in china is diagnosed on the basis of symptoms and is confirmed with serological testing. the geographical distribution, incidence, and case fata ... | 2011 | 21628481 |
| presence of activated airway t lymphocytes in human puumala hantavirus disease. | hantaviruses cause two clinical syndromes: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). the clinical spectrum in hfrs also often involves respiratory symptoms. as information about the pulmonary pathogenesis in hfrs is limited, we aimed to further study the local airway immune response in the lower airways. | 2011 | 21436245 |
| serum levels of inflammatory and regulatory cytokines in patients with hemorrhagic fever with renal syndrome. | abstract: background: hantaviruses are the causative agents of two zoonotic diseases: hemorrhagic fever with renal syndrome (hfrs) and hantavirus cardiopulmonary syndrome (hcps). the pathogenesis of hfrs is poorly understood. however, it has been suggested that immune mechanisms, including cytokines, might have an important role in hfrs pathogenesis. thus, the aim of our study was to investigate cytokine profiles in serum samples of hfrs patients from slovenia and explore a possible correlation ... | 2011 | 21605369 |
| environmental factors affecting transmission risk for hantaviruses in forested portions of southern brazil. | using ecological niche modeling approaches, we assessed suites of environmental factors to identify areas of transmission risk in forested regions of southern brazil. we studied all officially confirmed cases from the epidemiological surveillance system of the brazilian health ministry from atlantic forest and araucaria forest areas of southern brazil (n=264), during 1993-2008. among climate-related influences associated with hantavirus cases, winter precipitation and high photosynthetic mass (e ... | 2011 | 21605537 |
| glomerulonephritis emerging shortly after puumala hantavirus infection: a report on 7 patients. | aims: nephropathia epidemica (ne) is mild type of hemorrhagic fever caused by puumala (puu) hantavirus. renal biopsy typically shows acute tubulointerstitial nephritis and complete recovery is the usual outcome. we previously described 5 patients with membranoproliferative glomerulonephritis (mpgn) after acute ne. we now report on 7 more patients who developed biopsy-confirmed glomerulonephritis (gn) during the convalescent phase of ne. material and methods: we present case histories of 7 patien ... | 2011 | 21612760 |
| biogeographic and ecological regulation of disease: prevalence of sin nombre virus in island mice is related to island area, precipitation, and predator richness. | the relative roles of top-down and bottom-up forces in affecting disease prevalence in wild hosts is important for understanding disease dynamics and human disease risk. we found that the prevalence of sin nombre virus (snv), the agent of a severe disease in humans (hantavirus pulmonary syndrome), in island deer mice from the eight california channel islands was greater with increased precipitation (a measure of productivity), greater island area, and fewer species of rodent predators. in findin ... | 2011 | 21508614 |
| shared ancestry between a newfound mole-borne hantavirus and hantaviruses harbored by cricetid rodents. | discovery of genetically distinct hantaviruses in multiple species of shrews (order soricomorpha, family soricidae) and moles (family talpidae) contests the conventional view that rodents (order rodentia, family muridae and cricetidae) are the principal reservoir hosts and suggests that the evolutionary history of hantaviruses is far more complex than previously hypothesized. we now report on rockport virus (rkpv), a hantavirus identified in archival tissues of the eastern mole (scalopus aquatic ... | 2011 | 21632770 |
| Rodent-Borne Hantaviruses in Cambodia, Lao PDR, and Thailand. | In order to evaluate the circulation of hantaviruses present in southeast Asia, a large scale survey of small mammal species was carried out at seven main sites in the region (Cambodia, Lao People's Democratic Republic, and Thailand). Small scale opportunistic trapping was also performed at an eighth site (Cambodia). Using a standard IFA test, IgG antibodies reacting to Hantaan virus antigens were detected at six sites. Antibody prevalence at each site varied from 0 to 5.6% with antibodies detec ... | 2011 | 22124701 |
| pygmy rice rat as potential host of castelo dos sonhos hantavirus. | to study the dynamics of wild rodent populations and identify potential hosts for hantavirus, we conducted an eco-epidemiologic study in campo novo do parecis, mato grosso state, brazil. we detected and genetically characterized castelo dos sonhos virus found in a species of pygmy rice rat (oligoryzomys utiaritensis). | 2011 | 21801642 |
| pulmonary high-resolution computed tomography findings in nephropathia epidemica. | purpose: to evaluate lung high-resolution computed tomography (hrct) findings in patients with puumala hantavirus-induced nephropathia epidemica (ne), and to determine if these findings correspond to chest radiograph findings. materials and methods: hrct findings and clinical course were studied in 13 hospital-treated ne patients. chest radiograph findings were studied in 12 of them. results: twelve patients (92%) showed lung parenchymal abnormalities in hrct, while only 8 had changes in their c ... | 2011 | 21600717 |
| longitudinal analysis of the human t cell response during acute hantavirus infection. | longitudinal studies of t cell immune responses during viral infections in humans are essential for our understanding of how effector t cell responses develop, clear infection, and provide long-lasting immunity. here, following an outbreak of a puumala hantavirus infection in the human population, we longitudinally analyzed the primary cd8 t cell response in infected individuals from the first onset of clinical symptoms until viral clearance. a vigorous cd8 t cell response was observed early fol ... | 2011 | 21795350 |
| seroprevalence study in forestry workers of a non-endemic region in eastern germany reveals infections by tula and dobrava-belgrade hantaviruses. | highly endemic and outbreak regions for human hantavirus infections are located in the southern, southeastern, and western parts of germany. the dominant hantavirus is the bank vole transmitted puumala virus (puuv). in the eastern part of germany, previous investigations revealed tula virus (tulv) and dobrava-belgrade virus (dobv) infections in the respective rodent reservoirs. here, we describe a seroprevalence study in forestry workers from brandenburg, eastern germany, using igg elisa and imm ... | 2011 | 21611907 |
| Communicable diseases prioritized for surveillance and epidemiological research: results of a standardized prioritization procedure in Germany, 2011. | To establish strategic priorities for the German national public health institute (RKI) and guide the institute's mid-term strategic decisions, we prioritized infectious pathogens in accordance with their importance for national surveillance and epidemiological research. | 2011 | 21991334 |
| the cytoplasmic tail of hantavirus gn glycoprotein interacts with rna. | we recently characterized the interaction between the intraviral domains of envelope glycoproteins (gn and gc) and ribonucleoprotein (rnp) of puumala and tula hantaviruses (genus hantavirus, family bunyaviridae). herein we report a direct interaction between spike-forming glycoprotein and nucleic acid. we show that the envelope glycoprotein gn of hantaviruses binds genomic rna through its cytoplasmic tail (ct). the nucleic acid binding of gn-ct is unspecific, as demonstrated by interactions with ... | 2011 | 21807393 |
| detection of dobrava-belgrade hantavirus using recombinant-nucleocapsid-based enzyme-linked immunosorbent assay and sybr green-based real-time reverse transcriptase-polymerase chain reaction. | dobrava (dobv) hantaviruses belong to the genus hantavirus, family bunyaviridae, and are carried by yellow-necked and striped field mice. the goal of this study was to detect dobv using serological and genetic methods in apodemus rodents in hungary and in northern croatia. during the study period, a total of 125 apodemus sp. (67 a. agrarius, 58 a. flavicollis) were tested for the presence of hantaviruses, and 21 rodents (17%) were positive by rrt-pcr and/or elisa. we conclude that the prevalence ... | 2011 | 21573689 |
| puumala virus infection in syrian hamsters (mesocricetus auratus) resembling hantavirus infection in natural rodent hosts. | the mechanism of hantavirus persistent infection in natural hosts is poorly understood due to a lack of laboratory animal models. herein, we report that syrian hamsters (mesocricetus auratus) infected with puumala virus (puuv) at 4weeks old show persistent infection without clinical symptoms for more than 2months. igg and igm antibodies against the viral nucleocapsid protein and neutralizing antibody were first detectable at 14days postinoculation (dpi) and maintained through 70dpi. viral rna wa ... | 2011 | 21672562 |
| molecular evolution of azagny virus, a newfound hantavirus harbored by the west african pygmy shrew (crocidura obscurior) in cote d'ivoire. | abstract: background: tanganya virus (tgnv), the only shrew-associated hantavirus reported to date from sub-saharan africa, is harbored by the therese's shrew (crocidura theresae), and is phylogenetically distinct from thottapalayam virus (tpmv) in the asian house shrew (suncus murinus) and imjin virus (mjnv) in the ussuri white-toothed shrew (crocidura lasiura). the existence of myriad soricid-borne hantaviruses in eurasia and north america would predict the presence of additional hantaviruses ... | 2011 | 21798050 |
| hantavirus pulmonary syndrome: when should you consider this diagnosis? | 2011 | 21670936 | |
| landscape genetics highlights the role of bank vole metapopulation dynamics in the epidemiology of puumala hantavirus. | rodent host dynamics and dispersal are thought to be critical for hantavirus epidemiology as they determine pathogen persistence and transmission within and between host populations. we used landscape genetics to investigate how the population dynamics of the bank vole myodes glareolus, the host of puumala hantavirus (puuv), vary with forest fragmentation and influence puuv epidemiology. we sampled vole populations within the ardennes, a french puuv endemic area. we inferred demographic features ... | 2011 | 21819469 |
| hla associated hemorrhagic fever with renal syndrom disease progression in slovenian patients. | mhc class i and class ii genes regulate the balance between appropriate aggressive responses and invading pathogens while minimising the destruction of host tissue. several studies have shown that in hfrs patients the disease outcome is determined by a complex interaction between the virus, immunopathologic and human genetic factors. in slovenia the severity of the disease caused by puuv is significantly lower than that of hfrs due to dobv. we have determined 23 different hla-b and 12 different ... | 2011 | 21775516 |
| spatial structure of rodent populations and infection patterns of hantavirus in seven villages of shandong province from february 2006 to january 2007. | hemorrhagic fever with renal syndrome (hfrs) is endemic in junan county, shandong province, china. we conducted geographic information system (gis)-based spatial analysis with the objective of estimating the spatial distribution of rodent populations and their hantavirus infection patterns, to describe the spatial relationships of hantavirus strains in small ecological areas and to identify key areas in endemic areas of hfrs for future public health planning and resource allocation. | 2011 | 21740769 |
| dynamics of hantavirus infection in peromyscus leucopus of central pennsylvania. | abstract hantaviruses are distributed throughout the united states and are the etiologic agents for hantavirus pulmonary syndrome and hemorrhagic fever with renal syndrome. hantavirus genotypes and epidemiologic patterns vary spatially across the united states. while several longitudinal studies have been performed in the western united states, little is known about the virus in the eastern united states. we undertook a longitudinal study of hantaviruses in the primary rodent reservoir host in c ... | 2011 | 21756028 |
| puumala virus infection: radiologic findings. | a 33-year-old male patient was admitted to our nephrology department with rapidly deteriorating general health, fever, respiratory difficulties, and acute renal failure. computed tomography of the thorax revealed interstitial edema with thickening of the interlobular septa, peribronchial cuffing, ground-glass opacities, and small pleural and pericardial effusions. polymerase chain reaction tests verified puumala virus infection. the patient recovered with supportive treatment. hantavirus infecti ... | 2011 | 20736852 |
| non-human primates in outdoor enclosures: risk for infection with rodent-borne hantaviruses. | different species of non-human primates have been exploited as animal disease models for human hantavirus infections. to study the potential risk of natural hantavirus infection of non-human primates, we investigated serum samples from non-human primates of three species living in outdoor enclosures of the german primate center (gpc), göttingen, located in a hantavirus endemic region of central germany. for that purpose we used serological assays based on recombinant antigens of the bank vole (m ... | 2011 | 20727685 |
| genetic evidence for the presence of two distinct hantaviruses associated with apodemus mice in croatia and analysis of local strains. | in europe, dobrava-belgrade (dobv), saaremaa (saav), and puumala (puuv) viruses are known to cause hemorrhagic fever with renal syndrome (hfrs). all three hantaviruses are now found in croatia. lung tissue samples of 315 apodemus mice trapped in 2003-2004 were screened for the presence of hantaviral n-ag and 20 mice (6.3%) were found either strongly positive or weak/suspected-positive. partial sequences of hantavirus m and s segments were recovered by rt-pcr from six mice and subjected to (phylo ... | 2011 | 21108346 |
| detection of crimean-congo hemorrhagic fever, hanta, and sandfly fever viruses by real-time rt-pcr. | the development of sensitive and specific nucleic acid diagnostic assays for viral pathogens is essential for proper medical intervention. this chapter describes four fluorescence-based pcr assays to detect the crimean-congo hemorrhagic fever (cchfv), andes (andv), hantaan (hanv), and sandfly fever sicilian (sfsv) viruses. these assays are based on species-specific hydrolysis probes targeting the nucleocapsid protein gene for cchfv and sfsv and the glycoprotein gene for andv and hanv. all four a ... | 2011 | 21116810 |
| a possible case of hantavirus infection in a borneo orangutan and its conservation implication. | natural infection of hantavirus in orangutans has never been reported. | 2011 | 20722771 |
| prospective study on ocular findings in acute puumala hantavirus infection in hospitalised patients. | to appraise the ocular manifestations of puumala hantavirus evoked haemorrhagic fever with renal syndrome nephropathia epidemica (ne) and to clarify the mechanisms of ocular changes in the largest series of patients examined to date. | 2011 | 20679079 |
| hantavirus protein interactions regulate cellular functions and signaling responses. | rodent-borne pathogenic hantaviruses cause two severe and often lethal zoonotic diseases: hemorrhagic fever with renal syndrome (hfrs) in eurasia and hantavirus cardiopulmonary syndrome (hcps) in the americas. currently, no us fda-approved therapeutics or vaccines are available for hfrs/hcps. infections with hantaviruses are not lytic, and it is currently not known exactly why infections in humans cause disease. a better understanding of how hantaviruses interfere with normal cell functions and ... | 2011 | 21171876 |
| environmental risk factors for haemorrhagic fever with renal syndrome in a french new epidemic area. | summaryin france, haemorrhagic fever with renal syndrome (hfrs) is endemic along the belgian border. however, this rodent-borne zoonosis caused by the puumala virus has recently spread south to the franche-comté region. we investigated the space-time distribution of hfrs and evaluated the influence of environmental factors that drive the hantavirus reservoir abundance and/or the disease transmission in this area. a scan test clearly indicated space-time clustering, highlighting a single-year (20 ... | 2011 | 20822577 |
| development and application of a flow cytometric potency assay for dna vaccines. | we have developed a rapid, reliable, and sensitive quantitative flow cytometric assay to measure the in vitro potency and stability of dna vaccines to be delivered either by particle-mediated epidermal delivery (pmed) or by electroporation. the method involves transfecting cells with test dna and comparing the measured antigen expression to that generated with expression from known quantities of reference material dna. the assay was adapted for performance under good laboratory practice (glp) gu ... | 2011 | 21219978 |
| using geographic information system-based ecologic niche models to forecast the risk of hantavirus infection in shandong province, china. | hemorrhagic fever with renal syndrome (hfrs) is an important public health problem in shandong province, china. in this study, we combined ecologic niche modeling with geographic information systems (gis) and remote sensing techniques to identify the risk factors and affected areas of hantavirus infections in rodent hosts. land cover and elevation were found to be closely associated with the presence of hantavirus-infected rodent hosts. the averaged area under the receiver operating characterist ... | 2011 | 21363991 |
| procalcitonin in hantavirus infections. | in hantavirus infections levels of serum leukocytes or c-reactive protein are usually elevated to levels found in serious bacterial infections. however, procalcitonin in patients infected with hantavirus has not yet been discussed in the literature. a total of 29 adult patients with hantavirus infection, 30 with sepsis, and 19 with tick-borne encephalitis were included in this observational retrospective study. the median procalcitonin level in patients with hantavirus infection was 0.53 µg/l (r ... | 2011 | 21344982 |
| rodent community structure and andes virus infection in sylvan and peridomestic habitats in northwestern patagonia, argentina. | modifications of natural habitat in peridomestic rural areas could affect original rodent community composition, diversity, and evenness. in zoonoses such as hantavirus pulmonary syndrome, the presence of a diverse community can dilute the impact of the principal reservoir, reducing risk to humans. the goal of this study was to examine rodent community composition, abundance of andes virus (andv) host (oligoryzomys longicaudatus), andv prevalence, and temporal variability associated with rural p ... | 2011 | 21332352 |
| genetic diversity of imjin virus in the ussuri white-toothed shrew (crocidura lasiura) in the republic of korea, 2004-2010. | recently, imjin virus (mjnv), a genetically distinct hantavirus, was isolated from lung tissues of the ussuri white-toothed shrew (crocidura lasiura) captured near the demilitarized zone in the republic of korea. to clarify the genetic diversity of mjnv, partial m- and l-segment sequences were amplified from lung tissues of 12 of 37 (32.4%) anti-mjnv igg antibody-positive ussuri white-toothed shrews captured between 2004 and 2010. a 531-nucleotide region of the m segment (coordinates 2,255 to 2, ... | 2011 | 21303516 |
| hantavirus outbreak in western europe: reservoir host infection dynamics related to human disease patterns. | within europe, puumala virus (puuv) is the causal agent of nephropathia epidemica (ne) in humans, a zoonotic disease with increasing significance in recent years. in a region of belgium with a historically high incidence of ne, bank voles (the puuv reservoir hosts), were monitored for puuv igg antibody prevalence in nine study sites before, during, and after the highest ne outbreak recorded in belgium in 2005. we found that the highest numbers of puuv igg-positive voles coincided with the peak o ... | 2011 | 20450527 |
| Modification of the adenoviral transfer vector enhances expression of the Hantavirus fusion protein GnS0.7 and induces a strong immune response in C57BL/6 mice. | Hantavirus glycoproteins (Gn and Gc) are significant components of vaccines for haemorrhagic fever with renal syndrome (HFRS); however, they are not effective due to weak immunogenicity and low levels of production in expression systems. To circumvent this problem, a 0.7-kb fragment of the S segment was fused to Gn, and a hybrid CAG promoter/enhancer in conjunction with (or without) the WPRE (Woodchuck hepatitis virus post-transcriptional regulatory element) was used to improve the expression of ... | 2012 | 22015676 |
| [study on comprehensive monitoring of mouse and effect of hemorrhagic fever with renal syndrome vaccine in high prevalence areas of natural focus infectious disease of zhejiang province in 1994--2010]. | to study the comprehensive monitoring mechanism of mouse and the effect of hemorrhagic fever with renal syndrome (hfrs) vaccine in the high prevalence areas of natural focus infectious disease of zhejiang province in 1994 - 2010. | 2012 | 23363865 |
| Severe pulmonary involvement in a case attributed to domestically acquired seoul hantavirus in the United States. | Hantavirus is known to cause 2 distinct clinical syndromes: hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome. Seoul virus is an Old World hantavirus known to cause HFRS. We report a case attributed to domestically acquired Seoul hantavirus with prominent pulmonary involvement and a fatal outcome. | 2012 | 22042876 |
| Migration of norway rats resulted in the worldwide distribution of seoul hantavirus today. | Despite the worldwide distribution, most of the known Seoul viruses (SEOV) are closely related to each other. In this study, the M and the S segment sequences of SEOV were recovered from 130 lung tissue samples (mostly of Norway rats) and from six patient serum samples by reverse transcription-PCR. Genetic analysis revealed that all sequences belong to SEOV and represent 136 novel strains. Phylogenetic analysis of all available M and S segment sequences of SEOV, including 136 novel Chinese strai ... | 2012 | 22090114 |
| Do viral infections mimic bacterial sepsis? The role of microvascular permeability: A review of mechanisms and methods. | A dysregulated immune response and functional immunosuppression have been considered the major mechanisms of the bacterial sepsis syndrome. More recently, the loss of endothelial barrier function and resultant microvascular leak have been found to be a key determinant of the pathogenesis of bacterial sepsis. Whether a similar paradigm applies to systemic viral syndromes is not known. Answering this question has far-reaching implications for the development of future anti-viral therapeutic strate ... | 2012 | 22068147 |
| pathogenicity of a natural reassortant hantavirus cgrn9415 in newborn rats and newborn mice. | to better understand the pathogenicity and infectivity of a natural reassortant cgrn9415 generated from hantaan virus (htnv) and seoul virus (seov), cgrn9415, htnv 76-118, and seov l99 were used to infect newborn kunming (km) mice and newborn wistar rats. in km mice, there was no statistic difference between the death rate with cgrn9415 and that with l99, while 76-118 killed all mice even at low dosage; cgrn9415 killed all infected rats like l99 at the dosage of 105 ffu while no death occurred i ... | 2012 | 22258864 |
| Antiviral escape strategies developed by bunyaviruses pathogenic for humans. | New or re-emerging pathogens for humans have emerged outside of their usual endemic range during the last decade originating severe public health concern and economical losses. Climate changes have played a significant role in the emergence or re-emergence of arboviruses. Among these pathogens, several viruses belong to the Bunyaviridae family. This family is composed of RNA viruses grouped into five genera Orthobunyavirus, Hantavirus, Nairovirus, Phlebovirus and Tospovirus characterized by thei ... | 2012 | 22202109 |
| Isolation of sochi virus from a fatal case of hantavirus disease with fulminant clinical course. | Sochi virus, a novel genetic variant of Dobrava-Belgrade virus, was isolated in cell culture from a fulminant lethal case of hantavirus disease presenting with shock and combined kidney and lung failure. Sochi virus is transmitted to humans from host reservoir Apodemus ponticus and must be considered a life-threatening emerging agent. | 2012 | 22042875 |
| gene expression of estrogen receptors in pbmc from patients with puumala-virus infection. | abstract: the influence of estrogen signaling on infectious diseases is not fully known. males seem to be more susceptible to infections than females. this has also been noted for the scandinavian form of hemorrhagic fever with renal syndrome (hfrs) caused by puumala hantavirus (puuv). to investigate the differences in estrogen receptors in relation to sex and clinical severity, 20 patients (10 males, 10 females) with confirmed puuv infection was studied. real-time pcr was performed for analyzin ... | 2012 | 22266976 |
| [genotype of hantavirus in zhejiang province]. | to identify the genotype and clades of hantavirus (hv) in zhejiang province. | 2012 | 23547455 |
| [viral etiology of community acquired pneumonia among adults admitted to a general hospital]. | there is paucity of information about viral etiology of community acquired pneumonia in adults. | 2012 | 23282770 |
| seroepidemiological study reveals regional co-occurrence of lassa- and hantavirus antibodies in upper guinea, west africa. | to assess the public health relevance of lassa arenavirus and hantavirus infections in a subpopulation of recently febrile patients. | 2012 | 23279760 |
| atypical severe puumala hantavirus infection and virus sequence analysis of the patient and regional reservoir host. | nephropathia epidemica (ne) caused by puumala hantavirus (puuv) is a mild variant of haemorrhagic fever with renal syndrome, which is endemic in germany. we describe the case of a 67-year-old man initially presenting with acute bilateral angle-closure glaucoma, an atypical clinical presentation of puuv infection. subsequently, the patient developed a severe course of disease additionally complicated by profound hepatitis and interstitial nephritis, both phenomena which are rarely described in as ... | 2012 | 22958255 |
| Co-circulation of two Puumala hantavirus lineages in Latvia: A russian lineage described previously and a novel Latvian lineage. | Puumala hantavirus (PUUV) causes a mild form of haemorrhagic fever with renal syndrome in Europe. Seven genetic lineages of PUUV have thus far been recorded, which exhibit geographic structure within the distribution of its natural host, the bank vole (Myodes glareolus). This study presents evidence for two distinct PUUV lineages co-circulating in Latvia: one previously described from Russia and a novel one that appears to be endemic. The Latvian lineage (LAT) is considerably divergent and sever ... | 2012 | 22170553 |
| a unifying hypothesis and a single name for a complex globally emerging infection: hantavirus disease. | 2012 | 22068273 | |
| induction of protective immunity in a syrian hamster model against a cytopathogenic strain of andes virus. | andes virus (andv) is responsible for the hantavirus pulmonary syndrome cases in argentina and neighboring countries, with moderate to high case-fatality rates. andv has some particular features, which make it unique among other members of the hantavirus genus such as person-to-person transmission and causing a disease similar to hantavirus pulmonary syndrome in the hamster as an animal model. the kinetics of replication in vero e6 cells of an andv strain isolated in argentina, called andes/arg, ... | 2012 | 22095538 |
| sin nombre hantavirus decreases survival of male deer mice. | how pathogens affect their hosts is a key question in infectious disease ecology, and it can have important influences on the spread and persistence of the pathogen. sin nombre virus (snv) is the etiological agent of hantavirus pulmonary syndrome (hps) in humans. a better understanding of snv in its reservoir host, the deer mouse, could lead to improved predictions of the circulation and persistence of the virus in the mouse reservoir, and could help identify the factors that lead to increased h ... | 2012 | 22218940 |
| Immunogenic epitopes of Hantaviruses' N protein are restricted to conserved regions. | The Bunyaviridae virus family is composed by five genera, of which the Hantavirus genus is one of the most important representatives. Occasionally, these viruses can be transmitted to humans, giving rise to severe diseases that present high mortality rates. We analyzed the amino acid sequences of the nucleocapsid (N) proteins of 34 different hantaviruses to investigate the potential mechanisms involved in immunogenicity against hantaviruses. Immunogenic epitopes described in the literature throu ... | 2012 | 22201821 |
| hantavirus pulmonary syndrome clinical findings: evaluating a surveillance case definition. | abstract clinical cases of hantavirus pulmonary syndrome (hps) can be challenging to differentiate from other acute respiratory diseases, which can lead to delays in diagnosis, treatment, and disease reporting. rapid onset of severe disease occurs, at times before diagnostic test results are available. this study's objective was to examine the clinical characteristics of patients that would indicate hps to aid in detection and reporting. test results of blood samples from u.s. patients suspect ... | 2012 | 22214273 |