Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| sequence homology within the morbilliviruses. | double-stranded cdna synthesized from total polyadenylate-containing mrna extracted from monkey kidney cells infected with canine distemper virus (cdv) was cloned into the psti site of escherichia coli plasmid pbr322. clones containing cdv dna were identified by hybridization to a cdv-specific 32p-labeled cdna. a cdna clone containing an insert 1,700 base pairs (cdv 364) has been identified as the reverse transcript of the mrna coding for the nucleocapsid protein. the size of the mrna species co ... | 1985 | 3838193 |
| use of antibodies directed against synthetic peptides for identifying cdna clones, establishing reading frames, and deducing the gene order of measles virus. | a number of cdna clones complementary to measles virus mrna and 50s genome rna have been generated. these clones have been mapped by restriction enzyme analysis and were subsequently sequenced by the method of maxam and gilbert (a. m. maxam and w. gilbert, methods enzymol. 65:499-560, 1980). computer analysis of these dna sequences revealed open reading frames which potentially could code for a number of gene products. portions of these putative polypeptides were synthesized, and rabbit antibodi ... | 1985 | 3838350 |
| isolation and characterization of the measles virus f1 polypeptide: comparison with other paramyxovirus fusion proteins. | measles virus fusion (f) protein has been isolated by immunoadsorption to a complex of monoclonal antibodies bound to protein a-sepharose. the 41-kda f1 component of the fusion protein was obtained pure in high yield by preparative sds-polyacrylamide gel electrophoresis. the amino acid composition of the f1 chain was determined and the n-terminal sequence was analyzed for 40 residues. the structure determined is largely hydrophobic, with 24 residues of val, ile, leu, met, phe, or ala. comparison ... | 1985 | 3840623 |
| optimum age for measles immunization in malaysia. | 1985 | 3842727 | |
| [the hla antigen system and the immune response in measles vaccination]. | 1985 | 3843366 | |
| [determination of the biological activity of the measles virus by plaque formation]. | 1985 | 3843369 | |
| isolation of measles virus in vero cell cultures from the washed lymphocytic fraction of peripheral blood. | 1985 | 3855154 | |
| natural history of restricted synthesis and expression of measles virus genes in subacute sclerosing panencephalitis. | subacute sclerosing panencephalitis (sspe) is a slow infection caused by measles virus in which several years separate recovery from typical acute measles and the development of a slowly progressive neurological disease. we have investigated replication of measles virus in brain tissue obtained after the onset of neurological disease and in the terminal phase. with a hybridization tomographic technique that combines in situ hybridization with macroradioautographic screening of large areas of tis ... | 1985 | 3857631 |
| cytotoxic lymphocytes generated in vivo with acute measles virus infection. | we studied the generation of cytotoxic lymphocytes in adults during an outbreak of acute measles virus infection. nine patients were studied determining in particular whether virus-specific cytotoxic t lymphocytes could be directly detected in peripheral blood during this acute infection. the cytotoxicity of pbl was assayed against measles virus-infected and uninfected phytohemagglutinin-induced blast cells of matched and mismatched hla, a, b, and c types, in a standard 4-h 51cr release assay. t ... | 1985 | 3871183 |
| persistent viral infection affects tumorigenicity of a neuroblastoma cell line. | a mouse neuroblastoma cell line (clone ns20y) is highly tumorigenic in syngeneic a/j mice. when this clone was persistently infected with measles virus (ns20y/ms) it failed to grow or form tumors in conventional a/j or nude mice, even when large numbers of cells were inoculated. as doubling time, serum dependence, and anchorage-independent growth on agar did not differ significantly between ns20y and ns20y/ms, lack of tumorigenicity of the persistently infected cells is unlikely to be due to an ... | 1985 | 3871358 |
| improvement of atopic dermatitis following natural measles virus infection. four case reports. | five children with atopic dermatitis were studied during natural measles virus infection. four of the five patients showed temporary clearing of their skin lesions for 3 weeks following the infection. the reduction of cell-mediated immune responses caused by the measles virus may have played a role in the transient improvement of skin conditions in these children. evidence for t cell involvement in the clinical entity of atopic dermatitis is discussed. | 1985 | 3876791 |
| measles virus p gene codes for two proteins. | the entirety of the phosphoprotein gene of measles virus has been sequenced. the gene is composed of 1,657 nucleotides and specifies a 507-amino-acid protein (p). a second overlapping reading frame was predicted from the sequence and specifies a 186-amino-acid protein (c). through the use of antisynthetic peptide antibodies, we show that both proteins are expressed in virally infected cells. both proteins are expressed from a functionally bicistronic mrna through independent initiation of riboso ... | 1985 | 3882996 |
| demonstration of immunoglobulin in brains of ferrets inoculated with an sspe strain of measles virus: use of protein a conjugated to horseradish peroxidase. | measles virus-specific immunoglobulin g (igg) has been found in the brains of patients with subacute sclerosing panencephalitis (sspe), a slowly progressing central nervous system (cns) disease affecting children. igg/albumin ratios indicate that the antibodies are probably synthesized in the cns. in a ferret model system, protein a conjugated to horseradish peroxidase (prapx) was used to localize ig's in brains of animals inoculated with a cell associated strain of sspe. ig's were found in plas ... | 1985 | 3883686 |
| slow virus diseases of the central nervous system. | 1985 | 3893956 | |
| localization of measles virus antigens in subacute sclerosing panencephalitis in ferrets. | young adult male ferrets were inoculated intracerebrally (i.c.) with a cell-associated encephalitogenic subacute sclerosing panencephalitis (sspe) virus strain to study the pathogenesis of the disease at the ultrastructural level. most became acutely ill in 8-13 days. areas of the brain were examined with indirect immunoperoxidase labeling techniques to detect measles antigen. none of these animals showed the characteristic viral nucleocapsids or marked inflammatory response associated with sspe ... | 1985 | 3894788 |
| comparison of measles antihemolysin test, enzyme-linked immunosorbent assay, and hemagglutination inhibition test with neutralization test for determination of immune status. | sera were collected from 238 high-school students in prince edward island for the determination of immune status before an anticipated measles outbreak. in addition, history of vaccination status and measles infection was obtained. in the subsequent outbreak, 28 students did contract measles. specificity for hemagglutination inhibition (hi), antihemolysin (ah), and enzyme-linked immunosorbent assay (elisa) was 100%, compared with the neutralization test. corresponding sensitivity values for the ... | 1985 | 3897272 |
| measles virus matrix protein detected by immune fluorescence with monoclonal antibodies in the brain of patients with subacute sclerosing panencephalitis. | brain materials from four cases of subacute sclerosing panencephalitis were examined by immune fluorescence with monoclonal antibodies against five structural components of measles virus. all five antigens including the matrix component were present in the brain tissues of all cases. a defective vero cell-associated virus isolate from one of the cases produced all of the structural components except the matrix protein. | 1985 | 3897574 |
| [immunofluorescence reaction in studying humoral immunity in parotitis]. | 1985 | 3904211 | |
| antibodies specific for measles virus envelope antigens and autoantibodies in patients with chronic active hepatitis. | distinctly increased levels of antibodies to measles virus envelope antigens haemolysin and haemagglutinin were found in the sera of patients with chronic active hepatitis compared with a normal control group, using immunofluorescence and functional tests. similarly, a higher incidence of smooth muscle antibody of both igg and igm classes was observed in the patients and an important correlation was found between haemolysin antibodies specific for measles virus and smooth muscle antibody of igg ... | 1985 | 3905870 |
| replication of measles virus and localization of the viral antigens in long-term infection in organotypic cultures of hamster dorsal-root ganglion. | to investigate measles virus replication and expression of the individual viral proteins in differentiated various types of cells in neural tissues free from immunological effects organotypic cultures of hamster dorsal-root ganglion were employed. measles virus caused long-term infection up to 100 days in these cultures to which the virus was inoculated after they had been well-organized with myelination. electron microscopy and immunofluorescence technique demonstrated that the infection procee ... | 1985 | 3910882 |
| human mononuclear phagocytic cell interaction with some paramyxoviridae. | in consideration of the peculiar relationship between paramyxoviridae and immunocompetent system, we studied interactions of measles virus, newcastle-disease virus and respiratory syncytial virus with human monocytes and macrophages differentiated "in vitro". mononuclear cells were challenged with three different paramyxovirus at different multiplicities of infection. infected cells were then checked for expression of specific viral antigens by immunofluorescent test (fa). moreover, we have eval ... | 1985 | 3916331 |
| induction of chronic measles encephalitis in c57bl/6 mice. | chronic measles encephalitis was induced in c57bl/6 mice with the hamster neurotropic strain of measles virus when virus which had been passaged at low dilution in the brains of suckling c57bl/6 mice was inoculated intracerebrally into 1- to 6-month-old mice. one-third to two-thirds of mice surviving the acute infection were consistently found to develop chronic neurologic dysfunction within 3 weeks to 1 year post-infection. the acute mortality was higher in males than in females and showed a sl ... | 1985 | 3928806 |
| further data on the antigenic drift in subacute sclerosing panencephalitis (sspe). | serum and cerebrospinal fluid samples from 43 sspe patients were investigated by double radial immunodiffusion against a measles virus cell pack antigen. the pattern of immune precipitates suggested that antigenic drift may be involved in the pathogeny of persistent virus infection. | 1985 | 3934839 |
| malnutrition and seroconversion following measles immunization. | 1985 | 3937902 | |
| [induction of specific antibodies by measles vaccine. study in normal and undernourished infants 6 to 24-months old]. | 1985 | 3939164 | |
| severe measles: a reappraisal of the role of nutrition, overcrowding and virus dose. | the factors predisposing to severe measles, which accounts for a considerable fraction of the high infant and child mortality in the third world, are re-examined. the conviction that protein-energy-malnutrition (pem) aggravates measles is challenged by findings of prospective community-based studies. it was found that severe measles was not associated with pem but frequently accompanied overcrowding in guinea-bissau. secondary cases fared worse than index patients. among adequately nourished hos ... | 1985 | 3939698 |
| measles immunity in children before one year of age: a pilot study. | 1985 | 3965282 | |
| declining incidence of multiple sclerosis in the orkney islands. | the incidence of ms in the orkney islands has been updated from 1941 to september 21, 1983. since 1965, ms incidence rates have fallen significantly when compared with those for 1941 to 1964. alterations in age-specific prevalence, mean duration of illness, and mean age of the ms population are consistent with the decline in incidence of ms in recent years. although the reason for the decreasing incidence is uncertain, it is consistent with the hypothesis that ms may be caused by canine distempe ... | 1985 | 3982640 |
| measles outbreak in a pediatric practice: airborne transmission in an office setting. | in february 1981, a measles outbreak occurred in a pediatric practice in dekalb county, ga. the source case, a 12-year-old boy vaccinated against measles at 11 1/2 months of age, was in the office for one hour on the second day of rash, primarily in a single examining room. on examination, he was noted to be coughing vigorously. seven secondary cases of measles occurred due to exposure in the office. four children had transient contact with the source patient as he entered or exited through the ... | 1985 | 3982900 |
| [critical titre of hemagglutination inhibition antibodies in apparent measles infection]. | 1985 | 3995578 | |
| [measles & rubella hemagglutination inhibition antibodies in women of child-bearing age and the time of decline of maternally acquired antibodies among neonates and infants]. | 1985 | 3995579 | |
| measles virus matrix protein synthesized in a subacute sclerosing panencephalitis cell line. | measles virus generally produces acute illness. rarely, however, persistent infection of brain cells occurs, resulting in a chronic and fatal neurological disease, subacute sclerosing panencephalitis (sspe). evidence indicates that expression of the measles virus matrix protein is selectively restricted in this persistent infection, but the mechanism underlying this restriction has not been identified. defective translation of matrix messenger rna has been described in one sspe cell line. this r ... | 1985 | 4001938 |
| replication of measles virus in human lymphocytes. | replication of measles virus was restricted in human peripheral blood mononuclear cells (pbmc). however, in in vitro-infected, unstimulated cells, active synthesis of viral rna and proteins occurred, while the release of infectious virus could not be detected. stimulation with pha caused a productive infection cycle comparable to the lytic infection. replication of viral rna was demonstrated in both t and b cells, and in both okt4+- and okt8+-depleted t cell subsets. the presence of measles viru ... | 1985 | 4009115 |
| comparison of messenger rnas induced in cells infected with each member of the morbillivirus group. | virus-specific mrnas radiolabelled with [32p]orthophosphate in the presence of actinomycin d were extracted from the cytoplasm of vero cells infected with each of the known morbilliviruses: measles virus, canine distemper virus, rinderpest virus, and peste des petits ruminants virus. when analysed on denaturing agarose-formaldehyde gels the major rna species from all viruses in the group were identical, except for canine distemper virus where one of the virus-specific mrnas (mrna 5), which proba ... | 1985 | 4013082 |
| effect of measles virus antibodies on a measles sspe virus persistently infected c6 rat glioma cell line. | maintenance of measles (sspe-lec) virus persistently infected c6 rat glioma cells in medium containing polyclonal measles antiserum resulted in the loss of detectable expression of all measles virus proteins. removal of these cells from antiserum, however, led to a re-expression of virus proteins and the production of infectious virus. cloning of antibody-modulated non-expressing cells in the presence of antiserum showed that re-expression of virus proteins was not due to an incomplete curing pr ... | 1985 | 4020346 |
| measles virus encephalitis in ferrets as a model for subacute sclerosing panencephalitis. | young adult ferrets were used as experimental animals to study subacute sclerosing panencephalitis (sspe). when cells infected with cell-associated measles virus strains isolated from sspe patients were inoculated intracerebrally (i.c.) into ferrets, they developed an acute encephalitis and died within 1 to 3 weeks without detectable antibody formation. immunization with live measles vaccine 5 weeks before i.c. inoculation changed the course of the infection in about 50% of the ferrets. these an ... | 1985 | 4021436 |
| response to measles vaccine in haitian infants 6 to 12 months old. influence of maternal antibodies, malnutrition, and concurrent illnesses. | to study the factors affecting the serologic response to measles vaccination, we evaluated 595 haitian infants from 6 through 12 months of age, and their mothers, at the beginning of an immunization program. thirty-four per cent of the infants had preexisting serologic evidence of measles infections by 11 months of age. among infants more than nine months of age, those who had had measles had a significantly lower nutritional status than those who had not (p less than 0.01). after vaccination, s ... | 1985 | 4022091 |
| possible association of measles virus infection with rheumatic fever. | 1985 | 4022093 | |
| rna sequence and transcriptional properties of the 3' end of the newcastle disease virus genome. | the 3' end of the genomic rna of newcastle disease virus (ndv) has been sequenced and the leader rna defined. using hybridization to a 3'-end-labeled genome, leader rna species from in vitro transcription reactions and from infected cell extracts were found to be 47 and 53 nucleotides long. in addition, the start site of the 3'-proximal mrna was determined by sequence analysis of in vitro [beta-82p]gtp-labeled transcription products. the genomic sequence extending beyond the leader region demons ... | 1985 | 4024452 |
| maturation of measles virus hemagglutinin glycoprotein. | the processing of measles virus hemagglutinin glycoprotein (h) in infected cells was studied by pulse-chase method and two-dimensional isoelectric focusing and sds-polyacrylamide slab gel electrophoresis. h glycoprotein was synthesized initially as polypeptides smaller than h glycoprotein present in the virions. they were then processed into a cohort of polypeptides of larger molecular size and with reduced charge. the change was associated with the expression of h glycoprotein on the cell surfa ... | 1985 | 4026582 |
| detection of measles virus rna in lymphocytes from peripheral-blood and brain perivascular infiltrates of patients with subacute sclerosing panencephalitis. | to clarify the relation between lymphocytes and measles virus in subacute sclerosing panencephalitis, we used in situ hybridization and a cloned measles virus dna probe, specific for nucleocapsid protein, to detect measles virus rna sequences in circulating lymphocytes and brain perivascular cuffs of patients with subacute sclerosing panencephalitis. seventy to 90 per cent of peripheral mononuclear cells from three such patients were found to contain measles virus rna sequences. in contrast, onl ... | 1985 | 4033727 |
| subacute sclerosing panencephalitis and lymphocytes. | 1985 | 4033729 | |
| measles outbreak in a day nursery. | 1985 | 4034911 | |
| antineurofilament antibody: an increased incidence in murine chronic measles encephalitis and the use of rat sciatic nerve sections as substratum. | virus-associated autoimmune phenomena were investigated in the hnt-c57bl/6 chronic measles encephalitic mouse system. the incidence of serum antineurofilament autoantibodies was studied by an indirect immunofluorescence procedure using a fitc-conjugated goat anti-mouse immunoglobulin. transversely cut cryostat sections of rat sciatic nerve post-treated with 1% triton x-100 was used as substratum and had the advantages of increased reliability and reproducibility of neurofilament (nf) antibody ti ... | 1985 | 4040532 |
| viral antibodies in twins with multiple sclerosis. | viral antibodies to measles, rubella, corona, vaccinia, and mumps viruses in serum and csf (and to epstein-barr virus in serum only) were studied in 24 twin pairs, both discordant and concordant for clinical ms. in pairs, csf antibody titers for rubella in ms monozygotic and dizygotic twins and for vaccinia in dizygotic twins were higher than for unaffected twins. increased csf titers among ms twins existed for measles, rubella, and vaccinia when pairing was ignored. among ms twins, serum rubell ... | 1985 | 4040615 |
| a rapid method for the purification of cytoplasmic paramyxoviral nucleocapsids. | a rapid method for the purification of cytoplasmic paramyxoviral nucleocapsids using a tabletop ultracentrifuge is described. the method yields materials equivalent in purity to materials purified by previously published procedures but requires only a fraction of the time (65 min vs 7-41.5 h). when used in conjunction with modern hypersensitive analytical techniques, the procedure provides equivalent or greater amounts of nucleocapsid for subsequent analysis or study. | 1985 | 4040923 |
| subacute sclerosing panencephalitis: measles virus matrix protein nucleic acid sequences detected by in situ hybridization. | subacute sclerosing panencephalitis (sspe) is characterized by a hyperimmune state toward the polypeptides of measles virus except the matrix (m) protein. using cloned (3h)-labeled complementary dna probes for in situ hybridization, we found the m protein and nucleocapsid (np) protein nucleotide sequences in glial cells and neurons of cryostat sections from two sspe brains. in one sspe brain, m protein was lacking, but the other measles polypeptides were present. igg and igm antibodies eluted fr ... | 1985 | 4058750 |
| m protein instability and lack of h protein processing associated with nonproductive persistent infection of hela cells by measles virus. | persistent infections such as subacute sclerosing panencephalitis (sspe) which do not produce infectious virus particles (nonproductive persistence) are often accompanied by a reduced steady-state amount of the viral matrix (m) protein and/or reduced hemadsorption activity. the possible causes of these aberrations associated with nonproductive persistence were investigated by following changes in the viral proteins with time in pulse-chase experiments. three hela cell lines persistently infected ... | 1985 | 4060585 |
| round cell variant of measles virus: spontaneous conversion from productive to cell-associated state of infection. | a subacute sclerosing panencephalitis strain of measles virus was found to be composed of two viral variants distinguishable by their cytopathic effects in vero cells. one of the variants was similar to defective cell-associated measles virus strains, whereas the other was highly productive of viral progeny but did not induce cell fusion. cloning of the variants by an agarose plaque procedure revealed a consistent and rapid interconversion of the variants into one another. while the mechanism of ... | 1985 | 4060592 |
| [hemolytic activity of the measles virus]. | 1985 | 4060703 | |
| measles infection and parkinson's disease. | a case-control analysis of parkinson's disease and infections in childhood was conducted in a cohort of 50,002 men who attended harvard college (cambridge, ma) or the university of pennsylvania (philadelphia, pa) between 1916 and 1950 and who were followed in adulthood for morbidity and mortality data. cases of parkinson's disease were identified from responses to mailed questionnaires and death certificates through 1978. four controls from the same population were selected for each case. a redu ... | 1985 | 4061437 |
| acute and delayed neurologic reaction to inoculation with attenuated live measles virus. | a japanese boy developed a high fever and a prolonged convulsion 11 days after inoculation with live measles vaccine. he had 4 more seizures during the next 6 mos. his eeg became transiently abnormal 14 mos later. antimeasles complement fixation, hemagglutination, and neutralization titers were elevated. | 1985 | 4061779 |
| detection of antibody to m protein of measles virus in patients with subacute sclerosing panencephalitis: a comparative study on immunoprecipitation. | consistent results have not been obtained yet on the presence of antibody to the m protein of measles virus in the sera of patients with subacute sclerosing panencephalitis (sspe). we performed a comparative study on various immunoprecipitation systems which appeared in the literature and found that the difference in the composition of the solubilizing buffer produced a large variety of results on the immunoprecipitation. [35s]methionine-labeled vero cells infected with the edmonston strain of m ... | 1985 | 4069017 |
| two common neuro-ophthalmic problems. optic neuritis and transient visual disturbances. | optic neuritis and transient visual disturbances are common and challenging neuro-ophthalmic problems. optic neuritis may occur during the course of several neurologic and systemic disorders and is characterized by reversible central visual loss. in many patients, signs and symptoms of multiple sclerosis occur after an episode of optic neuritis. although several risk factors for development of multiple sclerosis have been identified, the relationship between optic neuritis and multiple sclerosis ... | 1985 | 4070095 |
| [clinico-laboratory and seroepidemiological examinations of children immunized with live measles vaccine prepared from genetically homogeneous strain moscow-5]. | the results of the clinico-laboratory and epidemiological study of a newly developed live measles vaccine obtained from strain moscow-5, genetically homogeneous and cloned from strain ji-16, are presented. the data indicate that the vaccine obtained from strain moscow-5 is safe and possesses low reactogenicity and high immunological potency, thus meeting all requirements for vaccinal preparations. | 1985 | 4072497 |
| [indices of antimeasles humoral immunity in newborn infants in relation to the anamnesis of the puerperae]. | 1985 | 4080487 | |
| cell sonicates used in the analysis of how measles and herpes simplex type 1 virus infections influence vero cell mitochondrial calcium uptake. | brief sonication was used to rapidly prepare an in situ mitochondrial ca2+ uptake system from vero cells. this method yielded intact, functional mitochondria capable of accumulating and retaining approximately 50% of ca2+ available in the reaction mix. mitochondria in cells infected with herpes simplex type 1 virus exhibited a gradual decline in the initial ca2+ uptake rate, dropping to 65% of the control rate at the end of the 12-h lytic cycle. influence of a lytic measles virus infection on th ... | 1985 | 4084857 |
| [measles: optimum age and number of doses recommended for vaccination in brazil]. | 1985 | 4089457 | |
| sspe: epidemiological and serological features in a series of cases recorded between 1975 and 1984. | a number of 632 new cases of subacute sclerosing panencephalitis (sspe) could be detected during a 10-year survey. the continuous increase in the number of annual cases suggests a possible correlation with the changes induced in measles epidemiology by the initiation of compulsory vaccination. on the other hand, a comparison of epidemiological and serological characteristics revealed significant differences between the series of sspe cases recorded in the period preceding and in that following m ... | 1985 | 4090270 |
| spontaneous cytotoxicity against measles virus infected and k562 cells in normal subjects and subacute sclerosing panencephalitis. | spontaneous cytotoxicity towards lu106 (heteroploid human lung cell line) cells persistently and acutely infected with measles virus was studied in measles immune and nonimmune healthy subjects and sspe patients. k562 (myeloid cell line) cells were also used to detect the nk activity. specific cytotoxic responses for measles immune and nonimmune donors were not significantly different for both persistently and acutely measles infected lu106 cells indicating that our assay system detected nk acti ... | 1985 | 4091977 |
| [search for the detection of matrix (m) protein nucleotide sequences in subacute sclerosing panencephalitis (sspe) frozen brain tissue by in situ hybridization]. | 1985 | 4092388 | |
| sensorineural hearing loss following live measles virus vaccination. | infection with measles virus is a well-known cause of acquired hearing loss in children. although measles virus vaccination on occasion may result in many of the complications seen with naturally occurring measles, the occurrence of hearing loss following measles virus vaccination has yet to be reported. this is a case report of encephalitis and severe sensorineural hearing loss following a measles virus vaccination. | 1985 | 4093255 |
| in vitro effect of haemagglutination inhibition antibodies on measles virus replication. | children between the ages of 6 and 15 years were studied for the presence of serum levels of measles haemagglutination inhibition antibodies in relation to the continual measles epidemics in nigeria. the neutralization of edmonston b strain of live measles virus by such antibodies was also studied in vero cells. the results indicate that in vero cells, replication of the virus could be inhibited for up to 3 days by haemagglutination inhibition antibody at a concentration of 1:16. | 1985 | 4094569 |
| optimum age for measles immunization in malaysia. | a study was carried out at the university hospital, kuala lumpur, malaysia to determine the age-specific prevalence of measles infection by serology and the age specific-seroconversion rates following measles vaccination. the results show that the percentage of children with passively acquired measles antibodies decreased with increasing age till 3 to 5 months of age. from 12 months of age, the percentage of positivity increased sharply due probably to natural infection. the geometric mean antib ... | 1985 | 4095612 |
| measles virus infection without rash in childhood is related to disease in adult life. | the presence of measles specific antibodies is usually taken as evidence of typical measles in the past; in the present study it was regarded as evidence of infection with measles virus, but not necessarily of the common disease accompanied by a typical rash. the association between a negative history of measles in childhood and certain diseases later in life was investigated by a historical prospective method, based on school health records combined with self-reporting in adulthood, and tests f ... | 1985 | 2856946 |
| intranasal administration of chick embryo fibroblast edmonston-zagreb measles vaccine. | 1985 | 2857883 | |
| mixing measles vaccine with dpt and dptp. | 1985 | 2860353 | |
| infectious oral necrosis (cancrum oris) in nigerian children: a review. | the devastating orofacial gangrenous disease known as cancrum oris (noma) is still commonly seen in underprivileged nigerian children. these children are usually victims of such stressors as chronic malnutrition, numerous endemic communicable diseases and severe adverse physical conditions which may lead to depletion of their adaptive resources or produce physiological maladaptation to additional stressors. measles is the most common infection preceding the development of noma in nigerian childr ... | 1985 | 2861938 |
| the anticholinergic anti-parkinson drug norakin selectively inhibits influenza virus replication. | 1985 | 2417553 | |
| [effectiveness of the selective revaccination of schoolchildren against measles]. | the revaccination of 70% of schoolchildren, previously immunized with measles vaccines of 26 different batches with insufficient immunogenic potency, has led to the pronounced and stable increase of immunity in the corresponding groups of children and has decreased measles morbidity among them 20 times. | 1985 | 2930972 |
| the unchanging epidemiology and toll of measles in burma. | an evaluation of the public health importance of measles in burma was carried out in 1983 to formulate recommendations regarding the feasibility of incorporating measles vaccine into the country's expanded program on immunization (epi). a review of available medical, serological, and epidemiological data as well as a sample study of childhood deaths indicated measles is a major killer of infants and young children in burma. since 1978, there have been over 20,000 cases of measles per year, and ... | 1985 | 2931207 |
| membrane markers, target cell specificity, and sensitivity to biological response modifiers distinguish human natural cytotoxic from human natural killer cells. | in the present report, we provide evidence for the distinct existence of a human natural cytotoxic (hnc) cell. this hnc cell can be identified by the monoclonal antibody hnc-1a3 and by the absence of the t10 antigen, other antigenic markers being shared, at least in part, with natural killer (nk) cells, t cells, or monocytes. in addition, the hnc cell preferentially kills the ma-160 target, the herpes simplex virus-1-infected ma-160 cell line, and the two human tumor cell lines hep-2 and hf-2. i ... | 1985 | 2932473 |
| detection of two viral genomes in single cells by double-label hybridization in situ and color microradioautography. | double labeling and color microradioautography were used in a new method of hybridization in situ to identify different genes in individual cells. the method is based on the unequal penetration of 3h and 35s into two layers of nuclear track emulsion separated by a thin barrier film. hybridization of a 35s-labeled probe specific for one kind of gene results in silver grains over cells in both layers of emulsion; a 3h-labeled probe for a second gene provides grains only in the first layer of emuls ... | 1985 | 2981430 |
| growth of defective subacute sclerosing panencephalitis viruses in human neural cells and their neurovirulence in mice. | a defective subacute sclerosing panencephalitis (sspe) virus which had been passaged in human embryonic lung cells was transferred to cultures of three neural cell types: neuroblastoma, oligodendroglioma and glioblastoma. the growth characteristics of the virus in these cells were essentially similar to those in non-neural cells. on the other hand, a marked difference in neurovirulence was noticed for the virus grown in neural cells when examined by intracerebral inoculation into mice. the virus ... | 1985 | 2981973 |
| adherent cells suppress measles and herpes simplex i virus-induced blastogenesis of multiple sclerosis lymphocytes. | viral antigen-induced blastogenesis of lymphocytes from multiple sclerosis (ms) patients was investigated to determine if the responses were actively suppressed. we found that depletion of adherent cells increased measles and herpes simplex i virus antigen-induced transformation of ms lymphocytes. addition of indomethacin to cultures of unfractionated ms lymphocytes also caused an increase in viral antigen-induced responses. these two facts, plus finding that the cell type mediating the immunosu ... | 1985 | 2982912 |
| antiviral potency of adenosine analogues: correlation with inhibition of s-adenosylhomocysteine hydrolase. | for a series of adenosine analogues a close correlation (r = 0.986) was found between their antiviral potency (against vesicular stomatitis virus) and their inhibitory effects (ki/km) on s-adenosylhomocysteine (adohcy) hydrolase; thus, in order of increasing inhibitory potency for both virus replication and adohcy hydrolase activity: (s)-9-(2,3-dihydroxypropyl)adenine less than (rs)-3-adenin-9-yl-2-hydroxypropanoic acid (isobutyl ester) less than carbocyclic 3-deazaadenosine less than neplanocin ... | 1985 | 2988550 |
| enzyme immunoassay versus plaque neutralization and other methods for determination of immune status to measles and varicella-zoster viruses and versus complement fixation for serodiagnosis of infections with those viruses. | results by an enzyme immunoassay method (eia) performed at one serum dilution and results by indirect immunofluorescence (ifa) and hemagglutination inhibition (hi) tests performed at step dilutions were correlated with results by a neutralization test (50% plaque neutralization [pn]) performed at step dilutions on single serum samples for serologic evaluation of immunity status to measles virus. pn results were taken as true indicators of immunity, and the other tests were evaluated on that basi ... | 1985 | 2989325 |
| efficiency of in situ hybridization as a function of probe size and fixation technique. | in an attempt to improve fixation technique for viral rna detection by in situ hybridization, we have quantitatively compared the hybridization signal obtained when measles virus or visna virus infected cell cultures were fixed with eight different fixatives and hybridized with 35s-labeled virus-complementary dna probes of several size ranges. small probes (mean length, 70 bases) gave higher signals than larger probes (mean lengths 140, 350, and 780 bases) with all fixatives. this increase in si ... | 1985 | 2991313 |
| antivirus antibodies in myasthenia gravis. | serum antibodies to influenza a, measles, rubella, cytomegalovirus, varicella zoster, herpes simplex type 1, and mumps have been assayed in 104 patients with myasthenia gravis, grouped according to clinical features plus thymus pathology, and compared with matched controls. no significant differences in incidence or antibody titer were detected. in 37 patients with recent onset of symptoms, the incidence of antibody to coxsackieviruses b1-b6 was less than in controls. juvenile-onset cases also d ... | 1985 | 2991819 |
| a simple method to detect intrathecal production of specific antimeasles antibodies in cerebrospinal fluid during subacute sclerosing panencephalitis. | the intrathecal production of antimeasles antibodies was studied using the enzyme-linked immunosorbent assay in eight specimens of serum and cerebrospinal fluid (csf) from patients with clinical signs of subacute sclerosing panencephalitis (sspe). the test was performed using a 1:5 dilution of csf and a 1:2000 dilution of serum (ratio 1:400) in order to nullify the physiological gradient of immunoglobulins across the blood brain barrier (bbb). this procedure allowed a rapid and accurate assessme ... | 1985 | 2992867 |
| enhanced intranuclear expression of measles virus following exposure of persistently infected cells to cyclic amp. | the intracellular distribution of measles virus inclusion bodies in persistently infected human cells (av3a1/mv) changed markedly following continuous exposure to 3', 5' cyclic adenosine monophosphate (camp). when assayed by immunofluorescence, the number of cells with intranuclear virus inclusions increased from 5 to 10% to 80 to 90% after exposure to 1 mm-camp for 4 days. exposure of cells to camp also resulted in a twofold increase in the average number of inclusions in invaded nuclei. simila ... | 1985 | 2993491 |
| a survey of serum antibodies to eight common viruses in psychiatric patients. | serum antibody titres to eight neurotropic viruses were measured by enzyme immunoassay in 450 psychiatric in-patients and 143 controls. a seasonal variation in schizophrenic births was observed, with a peak incidence between march and april. both herpes simplex virus and cytomegalovirus antibody titres correlated with age and, when this was controlled for, no significant differences emerged between any patient group and the controls. mumps antibody titres were significantly lower in patients wit ... | 1985 | 2994791 |
| serum and csf antibody titres to seven common viruses in schizophrenic patients. | csf and matched serum antibody titres to seven common viruses were measured in 20 chronic schizophrenic patients, and 17 of these were age and sex-matched with orthopaedic controls. ct scans were carried out in patients and age and sex-matched radiological controls. there was a trend for csf viral antibody titres (except cmv, hsv and vzv) to be decreased in the patients compared to controls, statistically significant for mumps and igg. the csf/serum ratios showed a reduction in the patients, com ... | 1985 | 2994792 |
| serum free cultivation of lymphoid cells for virus and interferon production. | minor modifications of standard media have given a defined serum free medium for the production of polio virus and measles virus in lymphoblastoid cells and of namalva interferon in lymphoma cells. by usage of slightly modified standard roller equipment in the order of 2 x 10 12tcid-50 of polio virus and 4 x 10(9) iu of interferon can be produced on one roller rack. the produced poliovirus does comply with requirements for inactivated poliovaccine. | 1985 | 2995174 |
| discordance between transferrin receptor expression and susceptibility to lysis by natural killer cells. | expression of the transferrin receptor on target cell lines has recently been implicated as a determinant of susceptibility to cytolysis by natural killer (nk) lymphocytes. we have examined this proposed relationship in several ways. first, k562 (a cell line highly vulnerable to nk lysis) cells were grown for 24 h in the iron chelator desferrioxamine. under these conditions, the cells doubled their surface transferrin receptor expression as determined both by radioligand binding and surface bind ... | 1985 | 2995450 |
| paramyxovirus antigens in osteoclasts from paget's bone tissue detected by monoclonal antibodies. | the fluorescent antibody technique using both monoclonal and specific polyclonal virus antibodies was applied to investigate the nature of the inclusions seen in the abnormal osteoclasts associated with paget's bone disease. the results show that antigens of measles virus, simian virus 5 (sv5) and human parainfluenza virus type 3 (pf3) could be detected in the osteoclasts but not in control bone cells. measles and sv5 nucleoprotein (np) and haemagglutinin-neuraminidase (hn) antigens were apparen ... | 1985 | 2995550 |
| synthesis and biological activity of certain 6-substituted and 2,6-disubstituted 2'-deoxytubercidins prepared via the stereospecific sodium salt glycosylation procedure. | a number of 6-substituted and 2,6-disubstituted pyrrolo[2,3-d]pyrimidine 2'-deoxyribonucleosides were prepared by the direct stereospecific sodium salt glycosylation procedure. reaction of the sodium salt of 4-chloro-6-methyl-2-(methylthio)pyrrolo[2,3-d]pyrimidine (6a) or 4,6-dichloro-2-(methylthio)pyrrolo[2,3-d]pyrimidine (6b) with 1-chloro-2-deoxy-3,5-di-o-p-toluoyl-alpha-d-erythro-pentofuranose (9) provided the corresponding n7 2'-deoxy-beta-d-ribofuranosyl blocked derivatives (8a and 8c) whi ... | 1985 | 2995666 |
| a viral antigen-bearing cell line derived from culture of paget's bone cells. | this study documents the characteristics of a viral antigen-bearing cell line derived from co-culture of the bone from a 71-year-old woman with paget's disease of bone and hep-2 cells. the cell line has survived in continuous culture for 3 1/2 years and 185 subcultures. the cells are epithelioid in appearance, produce alkaline and acid phosphatase, increase alkaline phosphatase activity in response to 1,25-(oh)2-d3 and contain receptors for 1,25-(oh)2-d3. immunofluorescent studies utilizing anti ... | 1985 | 2996574 |
| specific identification of human papillomavirus type in cervical smears and paraffin sections by in situ hybridization with radioactive probes: a preliminary communication. | cervical papanicolaou smears and paraffin sections of biopsy specimens obtained from women attending dysplasia clinics were examined for viral dna sequences by in situ hybridization technique using 35s-labeled cloned recombinant dna probes of human papillomavirus (hpv) types 6, 11, and 16. these and one unrelated dna probe complementary to measles virus rna were labeled by nick translation using either one or two 35s-labeled nucleotides. the radiolabeled probes were reduced in size with dnase to ... | 1985 | 2997054 |
| nucleotide sequence of the entire protein coding region of canine distemper virus polymerase-associated (p) protein mrna. | the entire coding region of the polymerase-associated (p) protein gene of canine distemper virus has been sequenced. a single cdna clone which represents 98% of the mrna encoding this protein was used to determine the nucleotide sequence. the sequence predicts a major protein of 507 amino acids and a molecular weight of 54 936. there is also a second, overlapping, open reading frame with a start signal 21 bases downstream of the first aug which could code for a protein of 174 amino acids with a ... | 1985 | 3000106 |
| a simple method for increased recovery of purified paramyxovirus virions. | a simple method involving vigorous agitation of infected cell monolayers prior to collection of culture medium is described to greatly increase the recovery of purified virions of measles virus, respiratory syncytial virus and human parainfluenza virus. vigorous agitation of the flasks containing monolayers of infected cells increased the recovery of purified virions by at least 3- to 10-fold as judged by the intensity of [35s]methionine labeled viral proteins on sodium dodecyl sulphate-polyacry ... | 1985 | 3001119 |
| virus-specific polymeric immunoglobulin a antibodies in serum from patients with rubella, measles, varicella, and herpes zoster virus infections. | more than 85% of the immunoglobulin a (iga) antibodies in normal adult serum are monomeric (m-iga). by contrast, virus-specific iga is mainly polymeric (p-iga) in sera from patients with rubella, measles, and varicella. specific m-iga antibodies only reach quantitative significance in late convalescence. in patients with herpes zoster, on the other hand, a varying response was observed: in three of six sera, specific iga was absent or at a very low titer, whereas in the remaining three cases, a ... | 1985 | 3001129 |
| dna-mediated gene transfer efficiency is enhanced by ionizing and ultraviolet irradiation of rodent cells in vitro. i. kinetics of enhancement. | the enhancement effects of ionizing and ultraviolet radiation on the efficiency of dna-mediated gene transfer were studied. the established cell line, rat-2, consists of cells that are density-dependent contact-inhibited and produce flat monolayers in vitro. when these cells are infected with sv40 virus, a small fraction of cells becomes morphologically "transformed" due to the stable expression of the viral a-gene. rat-2 cells are competent for dna-mediated gene transfer, deficient in thymidine ... | 1985 | 3001817 |
| cerebrospinal fluid virus antibodies. a diagnostic indicator for multiple sclerosis? | specific antibody activities (antibody per weight unit igg) of serum and csf against a broad variety of viruses were compared in multiple sclerosis and certain inflammatory diseases of the central nervous system, e.g. neurosyphilis, as well as herpes simplex and zoster encephalitis. no "unspecific" antiviral activities within the csf compartment were found in the non-ms diseases. the most frequent antibodies locally produced were directed against measles, rubella and zoster antigens. a diagnosti ... | 1985 | 3003256 |
| rapid diagnosis of viral infections with fluorescent antisera. | viral immunofluorescence tests were performed with commercial antisera on 1046 specimens of respiratory, conjunctival corneal and dermal origin to compare the diagnostic effectiveness of direct examination by immunofluorescence with culture. the fluorescence assays were found to be highly sensitive for respiratory syncytial and measles virus in nasopharyngeal aspirates from children, but less satisfactory for other respiratory viruses. the test detected 38% of the adenovirus positives from conju ... | 1985 | 3005952 |
| the immunosuppressive effects of measles virus on t cell function--failure to affect il-2 release or cytotoxic t cell activity in vitro. | measles virus (mv) is known to depress t cell function. in order to determine whether this results from alteration in the production of, or response to, interleukin-2 (il-2) we studied the effect of in vitro infection with mv on human il-2 dependent t cell lines. mv produced a cytopathic productive infection in these cells. class i allospecific cytotoxic t cells retained their cytotoxic activity 48 h after infection. both cytotoxic and leu 3a/4a positive t cell lines continued to respond to il-2 ... | 1985 | 3156014 |
| coincidental fluctuations of humoral immunity and clinical progression in a patient with subacute sclerosing panencephalitis. | serum and cerebrospinal fluid were obtained from a 6-year-old male with subacute sclerosing panencephalitis (sspe). specimens were collected over a 9-month period beginning in the unusually acute phase and ending in a more quiescent phase of the disease. immune complexes, auto-antibodies and viral antibodies were measured by radio-immunoassays. fluctuations in these humoral immune parameters coincided with cessation of the acute phase of this disease. the results show that neurological changes i ... | 1985 | 3158724 |
| changes in plasma ige levels during complicated and uncomplicated measles virus infections. | plasma ige levels were measured in 214 samples from 182 peruvian patients with acute measles virus infections. plasma ige levels were significantly elevated early in infection compared to later time points. plasma levels of igg from the same patients rose during the same time period, whereas levels of iga and igm did not change. in patients with postmeasles encephalomyelitis, ige remained elevated longer than it did in patients either with uncomplicated measles or measles complicated by pneumoni ... | 1985 | 3160758 |
| [the strength of postvaccinal measles immunity in schoolchildren]. | 1985 | 2954275 | |
| [clinico-laboratory indices of children revaccinated against measles]. | 1985 | 2954276 |