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in africa, clues to a connection. 19816261474
epstein-barr viral dna: infectivity for human placental cells.purified dna of epstein-barr virus (ebv) is regularly infectious by means of the "calcium" method of transfection. cultured human placental cells exposed to ebv dna of two transforming strains, ff41 and b95, produce virus that is capable of converting normal b lymphocytes into established cell lines. after treatment with ebv (ff41) dna and ebv (hr-1) dna the placental cells display antigens associated with the productive viral cycle. the placental cells have not developed foci or other signs of ...19816259735
infection of human b lymphocytes with high multiplicities of epstein-barr virus: kinetics of ebna expression, cellular dna synthesis, and mitosis. 19816259815
collinear relationships of herpesvirus papio dna to epstein--barr virus dna. 19816259833
purification and characterization of human lysosomes from eb-virus transformed lymphoblasts. 19816258947
thymidine and hypoxanthine requirements of normal and malignant human cells for protection against methotrexate cytotoxicity. 19816257387
[epstein-barr virus in the environment of patients with hodgkin's disease]. 19816258815
[effect of age and sex on antibody formation]. 19816258817
herpes encephalitis after infectious mononucleosis. 19816258252
epstein-barr virus-induced polypeptides: a comparative study with superinfected raji, iudr-treated, and n-butyrate-treated p3hr-1 cells. 19816258314
primary epstein-barr virus infection in early childhood.in a prospective study 43 consecutive children in hospital, aged between 6 months and 7 years and displaying at least one of the clinical signs of infectious mononucleosis (im), were investigated for epstein-barr (eb) virus-specific igm antibodies by an indirect immunofluorescence test. on this basis eb virus infection was considered confirmed in 8 patients, each of whom had igm antibodies in the initial serum sample. in one additional patient, igm antibodies were only detected in a second sampl ...19816258487
fisher's syndrome associated with epstein-barr virus. 19816258542
development of suppressor t lymphocytes for epstein-barr virus-induced b-lymphocyte outgrowth during acute infectious mononucleosis: assessment by two quantitative systems.a system of 3h-thymidine incorporation by lymphocytes in culture for 3 wk has been utilized for quantitative assessment of the ability of t lymphocytes to inhibit outgrowth of autologous epstein-barr virus (ebv) transformed b lymphocytes. lymphocytes from ebv-seronegative individuals lack the ability to suppress outgrowth of autologous ebv-transformed b lymphocytes. this capability appears during the course of primary ebv-induced infectious mononucleases (im) as the atypical lymphocytosis is sub ...19816257313
epstein-barr-virus-carrying lymphoma in a patient with ataxia-telangiectasia.an undifferentiated lymphocytic lymphoma of mesenteric lymph nodes occurred in a young boy with ataxia-telangiectasia. two independent tests, epstein-barr virus (ebv)-crna/dna hybridisation and ebv dna/dna reassociation kinetic analysis, showed 53 and 68 ebv genome equivalents per cell respectively, which was compatible with an ebv-genome-carrying tumour. whether this was a polyclonal lymphoproliferation or a monoclonal tumour could not be determined owing to lack of suitable material. the prese ...19816257325
cyclosporin a promotes spontaneous outgrowth in vitro of epstein-barr virus-induced b-cell lines.cyclosporin a (csa) is a fungal metabolite which exerts profound effects on the immune system and has potential as a selective immuno-suppressive agent. clinical trials with human renal allograft recipients have confirmed this potential but there have been disturbing reports of lymphoma in a significant number of patients. despite extensive animal studies, the specificity of this drug for human lymphocyte subpopulations is largely unknown. we demonstrate here that in vitro csa has no apparent ef ...19816256660
cell-surface immunoglobulin and insulin receptor expression in an ebv-negative lymphoma cell line and its ebv-converted sublines.membrane ig and insulin receptors were assayed in the ebv-negative ramos lymphoma line and its ebv-converted sublines by surface fluorescence. 125i-protein a-binding assay, immunoprecipitation, and insulin receptor assay. the original ramos line expressed surface igm but not igd and had a low concentration of insulin receptors. ten of 10 converted lines expressed igd and a variable but usually high insulin-binding capacity. molecular weight analysis of immunoprecipitated ig showed the presence o ...19816256436
human b cell activation in vitro: augmentation and suppression by monocytes of the immunoglobulin production induced by various b cell stimulants. 19816256442
fulminant hepatic failure in childhood. 19816256033
childhood social environment and hodgkin's disease.the epidemiologic similarities between hodgkin's disease in the young and paralytic poliomyelitis suggest that hodgkin's disease may be a rare consequence of a common infection, with the probability of oncogenesis increasing with age at the time of infection. in a study of 225 cases and 447 controls 15 to 39 years of age, we investigated the association of hodgkin's disease with factors in childhood that influence age of exposure to infectious agents. risk among persons with five or more sibling ...19816255329
in search of hodgkin's disease virus. 19816255330
cytotoxic t cell recognition of epstein-barr virus-infected b cells. i. specificity and hla restriction of effector cells reactivated in vitro.the experiments show that the phenomenon of regression, seen exclusively in epstein-barr (eb) virus-infected cultures of mononuclear cells from eb virus antibody-positive donors, is mediated by cytotoxic t cells reactivated in vitro and specifically recognizing an eb virus-induced lymphocyte-detected membrane antigen lydma. thus, effector t cells from regressing cultures kill autologous eb virus-transformed cells but not autologous pokeweed mitogen-stimulates lymphoblasts nor any of a range of e ...19816170517
stimulation with autologous lymphoblastoid cell lines: lysis of epstein-barr virus-positive and -negative cell lines by two phenotypically distinguishable effector cell populations.human lymphocytes, stimulated in vitro for 6 days with x-irradiated or glutaraldehyde-treated autologous epstein-barr (eb) virus-transformed lymphoblastoid cell lines (lcl), are cytotoxic for autologous and allogeneic eb+ lcls as well as for several eb- cell lines that are also susceptible to lysis by interferon-activated natural killer (nk) cells. to determine whether the apparent nonspecific lysis mediated by lcl-stimulated cells is due to a mixture of effector cells directed against different ...19816170690
the transformation of adult but not newborn human lymphocytes by epstein barr virus and phytohemagglutinin is inhibited by interferon: the early suppression by t cells of epstein barr infection is mediated by interferon.in a previous study, it was demonstrated that t cells from adult individuals were able to suppress the transformation of b cells after infection by ebv. in this paper, it is shown that this suppression is mediated by interferon. thus, the suppression is abrogated by anti-interferon serum and mimicked by human leukocyte interferon. furthermore, the interferon is released in response to the virus-infected b cell, not the virus alone. the relevance of these results to previous clinical evidence, in ...19816161964
ecto-adenosine triphosphatase deficiency in cultured human t and null leukemic lymphocytes. a biochemical basis for thymidine sensitivity.cultured leukemic t and null lymphocytes are highly sensitive to growth inhibition by thymidine, as well as the other deoxynucleosides, deoxyguanosine and deoxyadenosine. by contrast, epstein-barr virus-transformed b lymphocytes are relatively resistant to deoxynucleosides. growth inhibition is associated with the development of high deoxyribotriphosphate pools after exposure to the respective deoxynucleotides. we show that malignant t and null lymphocytes are deficient in ecto-atpase activity. ...19816114965
epstein-barr virus rna vii: size and direction of transcription of virus-specified cytoplasmic rnas in a transformed cell line.at least three separate regions of the epstein-barr virus (ebv) genome encode rna in a cell line that is growth transformed and nonpermissively infected with ebv. six polyadenylylated cytoplasmic rnas have been identified from these three regions. an abundant rna 3.0-3.1 kilobases (kb) long is encoded by dna of the internal reiteration, ir, and dna that maps at 25.7-30 megadaltons. a second, abundant, 2.9-kb rna is primarily encoded by dna at 110-03 megadaltons but probably has a 3' end to the l ...19816112750
presence of elevated antibody titres to epstein-barr virus before hodgkin's disease.sera taken 12 and 21 months before the diagnosis of hodgkin's disease in two patients showed higher antibody levels to several epstein-barr-virus (ebv) antigens than did sera from eight matched controls. in one case the titres of all eb antibodies except that to nuclear antigen were raised in a serum sample obtained 45 months after the diagnosis was made. antibody levels to herpes simplex, cytomegalovirus, and varicella zoster viruses were normal in both cases. a third patient with histiocytic l ...19816112530
iga antibody, antibody-dependent cellular cytotoxicity and prognosis in patients with nasopharyngeal carcinoma.immunoglobulin fractions containing antibodies to the epstein-barr virus (ebv) were isolated from different sera and tested in the antibody-dependent cellular cytotoxicity (adcc) assay. all cytotoxic activity resided in the igg fraction. iga antibodies were not cytotoxic in this assay against cells expressing the ebv-induced membrane antigen complex. however, iga antibodies were able to block the igg-mediated adcc reaction, indicating that the iga and igg antibodies recognized the same evb-speci ...19816169655
interferon production and activation of non-specific effector cells by stimulation with lymphoblastoid cell lines in vitro.a population of non-specific effector lymphocytes is generated in response to stimulation with lymphoblastoid cell lines (lcl). these cytotoxic cells have an activity analogous to that exhibited by natural killer (nk) cells. when lymphoid cells lines established by transformation with epstein-barr virus (ebv) are used to stimulate autochthonous t-cell-enriched lymphocytes, the activity against the nk-sensitive target, k562, is increased up to 14-fold. the stimulated t lymphocytes produce interfe ...19816169656
cellular immune defects to epstein-barr virus-determined antigens in young males.three males with the x-linked lymphoproliferative syndrome (xlp) with hypo- or agammaglobulinemia following epstein-barr virus (ebv) infection and two males with the chronic mononucleosis syndrome were investigated for immune responses to ebv-determined antigens. males with xlp showed profound cellular immune defects. markedly diminished responses of natural killer cell and interferon-activated killer cell activities and impaired leukocyte migration inhibition responses to phytohemagglutinin wer ...19816171337
usages of interferon and interferon inducers in man: the second half of 1980. 19816171689
core (2'-5')oligoadenylate and the cordycepin analog: inhibitors of epstein--barr virus-induced transformation of human lymphocytes in the absence of interferon.the 3'-deoxyadenosine (cordycepin) analog of (2'-5')oligo(a) [(2'-5')oligoadenylate with a triphosphate at the 5' end], synthesized enzymatically from cordycepin 5'-triphosphate in lysed rabbit reticulocytes or l-cell extracts was (i) inhibitory to translation in lysed rabbit reticulocytes and (ii) metabolically stable in extracts of either l cells or c85-5c lymphoblasts. the 5' dephosphorylated (core) (2'-5')oligo(a) and the core cordycepin analog can replace human fibroblast interferon in prev ...19816171822
conditions for cytomegalovirus stimulation of lymphocytes.lymphocytes from healthy donors or from patients with chronic lymphocytic leukaemia were subjected to live or inactivated cytomegalovirus (cmv) or the mitogen phytohaemagglutinin. no early or late cmv antigens could be demonstrated in the lymphocytes, indicating that neither abortive nor replicative cmv infection takes place. only cells from cmv antibody-positive leukaemic and non-leukaemic donors were stimulated by cmv to dna synthesis, with a maximum on day 5. cells from all individuals respon ...19816171871
cytolytic human t-cell clones expressing epstein-barr virus specificity and hla restriction. 19816173309
[immunologic protection against epstein-barr virus induced oncogenesis: cooperative effect of lymphocytes, antibodies and interferon in healthy individuals (author's transl)].immunologic cytotoxicity against lymphocytes transformed or infected by epstein-barr virus (ebv) was mainly studied in an autologous in vitro system by 51cr release assay and ebv-determined nuclear antigen (ebv)-specific trypan blue exclusion method. the following results were obtained. 1. the ebv-transformed or ebv-infected lymphocytes were killed with high frequency, when they were incubated with autologous lymphocytes in the presence of ebv-positive autologous serum. exposure to lymphocytes a ...19816175556
staphylococcal protein a enhances natural killing activity against lymphoid tumor cell lines.protein a (pa), was found to augment the natural killing (nk) activity of peripheral blood lymphocytes against burkitt's lymphoma-derived raji and daudi cells. this pa-enhanced nk activity was determined using the 51chromium release microcytotoxicity assay. it was found that the increased nk activity was dependent on the concentration of pa used. in addition, the pa-enhanced nk activity was dependent on the time of incubation of pa with the effector and target cell mixture. pretreatment of effec ...19816172391
epstein-barr virus strain- and group-specific antigenic determinants detected by monoclonal antibodies.in order to differentiate epstein-barr virus (ebv) strains by serological markers we prepared hybridomas producing monoclonal antibodies against polypeptides of the qimr-wil ebv strain. these monoclonal antibodies were screened by indirect immunofluorescence techniques. immunoprecipitation using ebv-positive monoclonal antibodies and the protein a method, of 125i-labelled polypeptides from purified qimr-wil ebv particles, revealed that a series of monoclonal antibodies against the four main surf ...19816172392
reactivation of epstein-barr virus-specific cytotoxic t cells by in vitro stimulation with the autologous lymphoblastoid cell line.unfractionated mononuclear (um) cells and t cells freshly prepared from the blood of adult donors were co-cultivated in microtest plate wells with progressively lower numbers of cells from the autologous eb-virus-transformed b-cell line. the fresh cells present in co-cultures from eb virus antibody-negative (seronegative) donors regularly facilitated autologous cell line outgrowth, monitored after 4 weeks, whereas outgrowth was markedly inhibited in the corresponding co-cultures from seropositiv ...19816169663
in vitro induction of hla-restricted cytotoxic t lymphocytes against autologous epstein-barr virus transformed b lymphoblastoid cell line.cytotoxic t lymphocytes (ctl) against autologous ebv-transformed b lymphoblastoid cell line (lcl) were induced in vitro by culturing peripheral blood lymphocytes (pbl) of healthy donors together with mitomycin c-treated autologous lcl for 6 days. the cytotoxic cells developed only from the e-rosette-positive fraction but not from the negative fraction of pbl. these ctl killed autologous lcl but not pwm-stimulated autologous pbl. in addition, the ctl killed allogeneic lcl when at least 1 of the h ...19816163815
t lymphocyte-mediated cytotoxicity against autologous ebv-genome-bearing b cells.we have stimulated human peripheral blood lymphocytes in vitro with autologous ebv-infected or noninfected b cells. a cytotoxic response was obtained only when virally infected cells were used. the activity of the effector cells was restricted by the major histocompatibility complex and was directed against ebv-genome-bearing targets. the highest cytolytic response was obtained when lymphocytes of individuals previously exposed to the virus (ebv-vca positive) were used. lymphocytes of noninfecte ...19816163816
epstein-barr virus rna. vi. viral rna in restringently and abortively infected raji cells.nuclear and polyadenylated rna fractions of raji cells are encoded by larger fractions of epstein-barr virus dna (35 and 18%, respectively) than encode polyribosomal rna (10%). polyribosomal rna is encoded by dna mapping at 0.05 x 10(8) to 0.29 x 10(8), 0.63 x 10(8) to 0.66 x 10(8), and 1.10 x 10(8) to 0.03 x 10(8) daltons. an abundant, small (160-base), non-polyadenylated rna encoded by ecori fragment j (0.05 x 10(8) to 0.07 x 10(8) daltons) is also present in the cytoplasm of raji cells. after ...19816165836
epstein-barr virus and herpesvirus saimiri: sensitivity to interferons and interferon inducers.the in vitro sensitivity of oncogenic herpesviruses, epstein-barr virus (ebv), and herpesvirus saimiri (hvs) to human interferon produced by normal human leukocytes (le), lymphoblastoid cell lines (lyi), and diploid fibroblasts (fi) was studied. four virus strains were used: hvs s295c, the highly oncogenic hvs s396-o, the transforming b95-8 strain of ebv, and the nontransforming p3hr1 strain of ebv. all interferons were active when applied to the cells after absorption of hvs and p3hr1-ebv, alth ...19816165852
induction of several new antigens, including a t cell-associated antigen, following conversion of the ebv-negative b cell line ramos to ehrb-ramos by epstein-barr virus. 19816166685
human lupus inclusions and interferon.raji cells, a human b lymphoblastoid cell line of burkitt lymphoma origin, formed lupus inclusions when grown in a medium conditioned by the growth of raji cells whose dna thymidine residues had been unifilarly (single-strandedly) substituted with bromodeoxyuridine. ultracentrifugation of this medium in excess of that required to remove epstein-barr virus and all other known mammalian viruses did not prevent the formation of the inclusions, and treatment of the conditioned medium with pronase de ...19816166984
antigenic differences between the membrane antigen polypeptides determined by different eb virus isolates.the antigenic specificities of the membrane antigen (ma) complex of three epstein-barr (eb) virus-producing cell lines have been compared by complete absorption of an anti-ma antiserum with p3hr-1 cells, followed by testing for residual anti-ma antibody activity against b95-8 and qimr-wil cells. indirect membrane immunofluorescence showed that the absorbed serum, which had lost the capacity to bind p3hr-1 cells, nevertheless gave bright staining on a small proportion of b95-8 cells. sds-polyacry ...19816167667
epstein-barr virus and chromosomal defects: a family study. 19816168880
interferon production by a human lymphoblastoid cell line (dg-75) free of the epstein-barr genome.a new lymphoblastoid cell line, dg-75, was investigated for its ability to produce interferon. dg-75 cells, previously shown to be free of epstein-barr virus genome and receptors, could be grown in submerged culture and could produce interferon in titers comparable to interferon produced by namalva cells. the interferon produced was similar in size to the namalva interferon as determined by gel filtration in ultrogel aca54. the dg-75 cells present a new source of large quantities of interferon w ...19816169305
abnormal responsiveness of rheumatoid b cells to epstein-barr virus infection in vitro. 19816118742
the distribution of a specific hla-b27-associated cell surface component on the tissues of patients with ankylosing spondylitis.in this paper, we report the presence on epstein-barr virus-transformed lymphoblastoid cell lines on platelets and on fibroblasts of an hla-b27-associated cell surface complex (antigenically related to some antigens of klebsiella k43 and k21) which is identical to or cross-reactive with the determinant present on the peripheral blood lymphocytes (pbl) of b27-positive patients with ankylosing spondylitis (as). by contrast, no klebsiella k43 markers could be demonstrated on the spermatozoa of b27+ ...19816171370
natural cell-mediated immunity during viral infections. 19816171387
lysis of human b-lymphocyte-derived lymphoma/leukemia cells of established cell lines by interferon-activated natural killer (nk) cells.cultured cell lines derived from malignant epstein-barr virus (ebv)-negative b cells, and representative of the more common types of naturally occurring non-hodgkin lymphomas and related leukemias, were found to be sensitive to lysis by human natural killer (nk) cells. the observed lysis of such cell lines was mediated by a population of interferon-augmentable, fcr-positive, non-adherent lymphoid cells, which were also able to kill the "standard" nk targets k562 and molt-4. when the nk susceptib ...19816171529
beta 2-microglobulin serum levels in infectious mononucleosis in childhood.a material of fifteen children aged 10 months to 15 years with infectious mononucleosis (im) was investigated. the diagnosis was established by demonstration of epstein-barr virus (ebv) specific igm antibodies. the serum concentrations of beta 2-microglobulin (beta 2-m) were significantly increased (p less than 0.01) in the first sample after onset of disease, compared to control groups of children with other infectious diseases and children without diseases. the serum concentration of beta 2-m ...19816171995
activation of the epstein-barr virus genome by 5-aza-cytidine in latently infected human lymphoid lines.recent studies indicate that gene expression in higher eukaryotes is accompanied by a decrease in the frequency of 5-methyl cytosine residues around the activated site (razin and riggs, 1980). 5-aza-cytidine (5-aza-c) is an analogue that reduces cytidine methylation in dna (jones and taylor, 1980) and has been reported to change the differentiation pattern of cultured mouse embryo cells (taylor and jones, 1979). we have tested its ability to activate the epstein-barr virus cycle in latently ebv- ...19816172387
antigen-specific antibody response induced in cultures of human blood lymphocytes in presence of polyethylene glycol.human peripheral blood lymphocytes respond in vitro to sheep red blood cells (srbc) with the appearance of numerous specific plaque-forming cells, if the epstein-barr virus (ebv) is added to the cultures together with the antigen. however, it was found that when polyethylene glycol (mol. wt. 6000) is included in the culture medium at a final concentration of 4%, antigen alone is able to induce an anti-srbc response, with clear hemolytic plaques of regular size. addition of ebv causes a further i ...19816173234
long-term t-cell-mediated immunity to epstein-barr virus in renal-allograft recipients receiving cyclosporin a.peripheral-blood mononuclear cells from renal-allograft patients receiving cyclosporin a (csa) were tested for their ability to produce t cells cytotoxic for eb-virus-infected b-cell targets in culture and compared with those from healthy seropositive subjects. whereas in the control cultures the proliferating foci of eb-virus-transformed b cells regressed after 2 weeks, no such regression was seen in cultures from csa-treated patients. these results indicate that patients receiving csa cannot m ...19816109048
what we could do with an ebv vaccine. 19816110958
kawasaki disease and epstein-barr virus. 19816112339
is histiocytic medullary reticulosis a fatal form of infectious mononucleosis? 19816113417
hodgkin's disease and epstein-barr virus. 19816113478
malaria, sex, and place of residence as factors in antibody response to epstein-barr virus in ghana, west africa.both epstein-barr virus and malaria have been implicated as possible factors in burkitt's lymphoma. to examine this implication a cross-sectional serological survey of an urban and a rural population in ghana was conducted. malaria was more prevalent in the rural areas, but there was no difference in antiviral capsid antigen (vca) antibody titres between urban and rural dwellers. there was also no correlation between anti-malaria antibody titres and anti-vca antibody titres. thus malaria does no ...19816113482
treatment of childhood combined epstein-barr virus/cytomegalovirus infection with oral bovine transfer factor.an illness lasting for two years, with recurrent fever, rash, abdominal pain, and arthralgia, developed in a four year old boy. he was found to have a combined epstein-barr virus and cytomegalovirus (cmv) infection. his symptoms, cmv in his urine, and an absent in vitro lymphocyte response to cmv antigen persisted for two years. after treatment with orally administered bovine transfer factor clinical symptoms and viruria disappeared and specific immunity to cmv developed. evaluation of this trea ...19816113484
cyclosporin a immunosuppression and epstein-barr antibody. 19816114256
epstein-barr virus and hodgkin's disease. 19816115129
effects of hydrocortisone on the synthesis of epstein-barr virus antigens in p3hr-1 cells.hydrocortisone was capable of enhancing the synthesis of epstein-barr virus antigens in producer p3hr-1 cells which had been induced with a 24-hr pulse of 5-iodo-2-deoxy-uridine. the effect of hydrocortisone was time dependent, suggesting that an early step in the induction process, and not the antigen synthesis itself, was influenced by the drug.19816115566
multiple sclerosis and epstein-barr virus. 19816118702
lack of regression in epstein-barr virus infected leucocyte cultures of nasopharyngeal carcinoma patients. 19816118752
presence of epstein-barr virus dna in tonsillar tissues.sera from 48 tonsillar carcinoma (tc) patients, 48 matched controls and 16 recurrent exudative tonsillitis (ret) patients were examined for the presence of epstein-barr virus (ebv) associated nuclear antigen (ebna), early antigen (ea) and virus capsid antigen (vca). higher prevalence and significantly higher antibody titres against all three ebv-associated antigens were observed in tc patients in comparison with controls and ret patients. patients suffering from anaplastic tc had higher titres o ...19816120637
striking similarities are exhibited by two small epstein-barr virus-encoded ribonucleic acids and the adenovirus-associated ribonucleic acids vai and vaii.the nucleotide sequence of the region of the epstein-barr virus genome that specifies two small ribonucleic acids (rnas), eber 1 and eber 2, has been determined. both of these rnas are encoded by the right-hand 1,000 base pairs of the ecori j fragment of ebv deoxyribonucleic acid. eber 1 is 166 (167) nucleotides long and eber 2 is 172 +/- 1 nucleotides long; the heterogeneity resides at the 3' termini. the eber genes are separated by 161 base pairs and are transcribed from the same deoxyribonucl ...19819279391
no activation of new initiation points for deoxyribonucleic acid replication in balb/c 3t3 cells transformed by kirsten sarcoma virus.balb/c 3t3 cells were transformed by kirsten sarcoma virus, and five clones were isolated in soft agar. average replicon sizes of the transformed cell lines were estimated by the method of fiber-autoradiography (j. a. huberman and a. d. riggs, j. mol. biol.32:327-341, 1968) and found to be the same size as the nontransformed 3t3 cells, analyzed in parallel. the results indicate that, unlike simian virus 40 and epstein-barr virus, kirsten sarcoma virus does not activate new initiation points for ...19819279389
initiation points for cellular deoxyribonucleic acid replication in human lymphoid cells converted by epstein-barr virus.replicon size was estimated in two epstein-barr virus (ebv)-negative human lymphoma lines, bjab and ramos, and four ebv-positive lines derived from the former ones by infection (conversion) with two viral strains, b95-8 and p3hr-1. logarithmic cultures were pulse-labeled with [3h]thymidine, and the deoxyribonucleic acid was spread on microscopic slides and autoradiographed by the method of huberman and riggs. after developing, replication forks were visualized as silver grain tracks on the autor ...19819279388
prolonged atypical illness associated with serological evidence of persistent epstein-barr virus infection.seven patients with prolonged atypical illness were followed up for more than a year. sera taken during that period showed significantly increased titres of igm antibodies against the viral capsid antigen (vca) of epstein-barr virus (ebv). in four of the patients antibodies to the r component of the early antigen (ea) complex of ebv were clearly detectable. only one of these seven patients had presented with symptoms of classic infectious mononucleosis. serological and clinical observations in t ...19826119490
serum antibody profile against herpesviruses in renal transplant recipients.forty-eight renal allograft recipients who had received a kidney graft 2 months to 8 years previously, were followed for the presence of serum antibody against cytomegalovirus (cmv), epstein-barr virus (ebv), herpes simplex virus (hsv), and varicella-zoster virus (vzv). these investigations were performed over a 6 months period and in at least two serum samples from each patient, obtained at intervals of 2-3 months. the presence of serological signs of recent or active infection were observed in ...19826100358
influence of ataxia telangiectasia gene dosage on bleomycin-induced chromosome breakage and inhibition of replication in human lymphoblastoid cell lines. 19826173245
immunity to epstein-barr virus in cyclosporin a-treated renal allograft recipients. 19826123746
stimulation of human lymphocytes with irradiated cells of the autologous epstein-barr virus-transformed cell line. i. virus-specific and nonspecific components of the cytotoxic response. 19826176332
stimulation of human lymphocytes with irradiated cells of the autologous epstein-barr virus-transformed cell line. ii. cytotoxic response to repeated stimulation. 19826176333
the increased natural killer sensitivity of epstein-barr virus (ebv)-superinfected raji cells is not due to the recognition of serologically determined ebv antigens by the effectors. 19826176334
epstein-barr virus-specific cytotoxic t-cell clones restricted through a single hla antigen. 19826176881
identification of epstein-barr virus strain differences with monoclonal antibody to a membrane glycoprotein.two monoclonal antibodies directed against epstein--barr virus (ebv)-induced membrane antigens (ma) were isolated in this study. on of the monoclonal antibodies, designated 2f5.6, was an igg2 which, as detected by membrane and fixed cell immunofluorescence, reacted with ma-positive lymphoblastoid cell lines that produced transforming ebv but not with the ma-positive p3hr-1 cell line that produced the lytic, nontransforming strain of this virus. this antibody precipitated the mr 320,000/350,000 g ...19826176998
antiviral effects of interferon on a somatic cell hybrid between two burkitt's lymphoma cell lines of different interferon sensitivities.a somatic cell hybrid between two human burkitt's lymphoma cell lines, raji and daudi, was infected with either epstein-barr virus or vesicular stomatitis virus after interferon treatment. raji cells are resistant to the antiviral effects of exogenously added interferon, whereas daudi cells are interferon sensitive. the raji-daudi hybrid showed an interferon sensitivity that was intermediary to that of the parental cells against both viruses.19826177642
production and characterization of monoclonal antibodies against the epstein-barr virus membrane antigen.five murine hybridoma lines that produce monoclonal antibodies against epstein-barr virus membrane antigen (ma) were established. immunoprecipitation experiments demonstrated that three of the antibodies precipitated both the 236,000 (236k) ma and the 212k ma. the other two antibodies precipitated the 86k ma. antibodies against the 236k-212k ma and the 86k ma mediated complement-dependent cytolysis of epstein-barr-virus-infected cells. the antibodies against the 86k ma neutralized both the b95-8 ...19826177865
interferon enhances the expression of epstein-barr virus early antigen in daudi cells.treatment of the burkitt lymphoma-derived cell line daudi with highly purified human interferon-alpha (ifn-alpha) increased up to 100-fold the number of cells expressing epstein-barr virus (ebv)-determined early antigen (ea) without inducing the synthesis of virus capsid antigen (vca), a late virus function. the increase in the number of ea-positive cells was proportional to interferon concentration up to 10(4) international units (iu)/ml. treatment of daudi cells with the same number of units o ...19826178801
evolution of infectious mononucleosis into epstein-barr virus carrying monoclonal malignant lymphoma.a monoclonal malignant lymphoma was diagnosed in a 30-month-old japanese boy, who, since the age of 12 months, had had chronic, recurrent infectious mononucleosis manifested by repeated episodes of severe cough and high fever accompanied by marked lymphadenopathy and hepatosplenomegaly and high serum epstein-barr virus (ebv) antibody titres. the diagnosis of burkitt's lymphoma was made when a cervical lymph-node biopsy specimen revealed massive proliferation of immature b-cells with starry-sky h ...19826123020
two distinct human cloned t cell lines that exhibit natural killer-like and anti-human effector activities.cloned t cell lines from mixed lymphocyte cultures stimulated with autologous epstein barr virus- (ebv) transformed lymphoblastoid cell line (lcl) cells were established using a limiting dilution technique in the presence of t cell growth factor (tcgf). the t cell lines included two distinct clones of cytotoxic t cells (tc) in addition to ebv-specific tc. a cytotoxic profile of one cloned line was similar to that of endogenous nk cells in peripheral blood. the other cloned tc line showed an anti ...19826120975
defective rheumatoid lymphocyte function assessed by epstein-barr virus infection in vitro. 19826128620
increased (2'-5')-oligo-a synthetase activity in patients with prolonged illness associated with serological evidence of persistent epstein-barr virus infection. 19826122038
epstein-barr-virus-specific t-cell memory in renal-allograft recipients under long-term immunosuppression.the level of long-term responsiveness of cytotoxic t cells to epstein-barr (eb) virus has been measured in peripheral-blood mononuclear-cell cultures from symptom-free renal-allograft recipients under conventional immunosuppression. 8 of 22 patients showed a profoundly impaired responsiveness when compared with health control donors. the allograft recipients as a group showed raised antibody titres to eb-virus capsid and early antigens, but within the group there was no clear relation between th ...19826122768
infectious mononucleosis and systemic lupus erythematosus. 19826122807
epstein-barr-virus-carrying lymphoma in cardiac transplant recipient. 19826123868
anti-human cytomegalovirus nuclear antigen antibodies of different immunoglobulin classes.igg, igm and iga immunoglobulin classes of antibodies to human cytomegalovirus nuclear antigens (cmna) were studied by the acid-fixed nuclear binding technique (afnb) and combined anti-complement immunofluorescence (combined acif). in acute cases of infectious mononucleosis (im) of human cytomegalovirus (hcmv) origin and in the so-called double virus infections (hcmv + epstein-barr virus), anti-cmna igm antibodies were detected. they were absent from both anti-hcmv positive sera of healthy donor ...19826124112
outbreak of burkitt's-like lymphoma in homosexual men.four cases of burkitt's-like lymphoma (undifferentiated, monoclonal, b-cell tumours) in homosexual men were seen in a 9-month period in san francisco. one tumour contained both epstein-barr-virus nuclear antigen (ebna) and cytomegalovirus (cmv) antigen. another tumour contained ebna, and a third contained no viral antigen, but ebna and cmv antigens were detected in the overlying epithelium. this outbreak widens the array of neoplasms affecting immunosuppressed homosexual men and provides further ...19826125777
rheumatoid lymphocyte defect revealed by cyclosporin a. 19826126656
no indication of active epstein-barr virus infection in two fatal cases of histiocytic medullary reticulosis. 19826126683
new clinical manifestations of epstein-barr virus infection. 19826128552
human anti-tetanus toxoid monoclonal antibody secreted by ebv-transformed human b cells fused with murine myeloma.an epstein-barr virus (ebv)-transformed human b cell line (b6) producing anti-tetanus toxoid (tt) antibody was fused with a nonimmunoglobulin (ig)-producing murine myeloma and selected in hypoxanthine-aminopterin-thymidine (hat) medium containing 10(-5) m ouabain. surviving cells were cloned by limiting dilution and confirmed as hybrids by karyotype analysis and g-11 staining. hybridomas were stable and secreted 10-fold more anti-tt antibody (igm kappa) than the human parental cell line. in addi ...19826092264
neutralization of lymphocyte immortalization by different strains of epstein-barr virus with a murine monoclonal antibody.a murine monoclonal antibody was raised against the b95-8 strain of epstein-barr virus (ebv), which was isolated from a case of mononucleosis after blood transfusion (hoffman et al. proc. natl. acad. sci. u.s.a. 77:2979, 1980). we provide evidence that neutralization of immortalization by this monoclonal antibody is virus specific, since its potency was inversely related to the dose of challenge virus. furthermore, the monoclonal antibody recognized antigens on viruses grown in human as well as ...19826182100
autoantibodies to nuclear antigens (ana): their immunobiology and medicine. 19826182766
detection of antibodies to epstein-barr virus antigens by enzyme-linked immunosorbent assay.an enzyme-linked immunosorbent assay (elisa) is described for the serologic analysis of antibodies to epstein-barr virus (ebv)-associated nuclear antigen (ebna), viral capsid antigen (vca), and early antigen (ea). the specificity of each of the elisas was demonstrated by the use of well-characterized human sera shown by immunofluorescence assay to be variously reactive for antibodies to one or more of the three viral antigens studied. the elisa for ebna was four to 256 times more sensitive than ...19826183369
heterogeneity of allogeneic restriction of human cytotoxic t cell clones specific for epstein barr virus.clones of human cytotoxic t cells (tc) specific for epstein barr virus (ebv) were isolated from peripheral blood lymphocyte (pbl) cultures stimulated repeatedly with autologous ebv-transformed lymphoblastoid cell line (lcl) cells in vitro. the method employed to clone ebv-specific tc was a limiting dilution technique utilizing t cell growth factor (tcgf). the ebv specificity of tc clones was determined by showing that they were significantly cytotoxic for autologous lcl cells but not for either ...19826173428
differential expression and serologically distinct subpopulations of human ia antigens detected with monoclonal antibodies to ia alpha and beta chains.studies with two monoclonal antibodies (da6.147 and da6.231) which react, respectively, with isolated human ia alpha and beta chains are reported. both antibodies detect epitopes expressed on all dr-heterozygous and dr-homozygous cell lines tested (n = 17) and bind to the epstein-barr virus-negative cell line ramos. ia subunit specificity of the antibodies was determined by an adaptation of an electroblot technique which transfers separated ia chains from polyacrylamide gels to nitrocellulose pa ...19826185348
[liver diseases in children]. 19826175880
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