Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| mosquito transposable elements. | most of the transposons so far characterized from mosquito genomes are retroelements which seem to be distributed worldwide. the juan transposons constitute a family of non-ltr retroelements, or line-retroposons, which are dispersed in the genomes of several mosquito species. three different juan subfamilies have been characterized, each being amplified in the genomes of many strains, if not all, of a given mosquito species. these subfamilies have been designated respectively juan-c in culex pip ... | 1994 | 7813911 |
| salivarectomy effects on the ingestion of brugia pahangi (nematoda: filarioidea) microfilariae by susceptible and refractory strains of aedes aegypti (diptera: culicidae). | ingestion of brugia pahangi buckley & edeson microfilariae by susceptible (liverpool) and refractory (bora-bora) strains of salivary duct-severed aedes aegypti (l.) was investigated. the transection at either the salivary common duct or the two lateral ducts of mosquitoes did not affect the mean number of microfilariae nor the mean amount of blood ingested per mosquito. these results indicated that mosquito salivary secretion did not have a chemotropic effect on microfilariae. | 1994 | 7815410 |
| synergism of mosquitocidal toxicity between cyta and cryivd proteins using inclusions produced from cloned genes of bacillus thuringiensis. | the toxicity to mosquito larvae of the parasporal body produced by bacillus thuringiensis subsp. israelensis and the pg-14 isolate of b. thuringiensis subsp. morrisoni is at least 20-fold greater than any of the four mosquitocidal proteins of which it is composed (cyta, cryiva, b, and d). this high toxicity is postulated to be due to synergistic interactions among parasporal proteins. however, this remains controversial because values reported for the specific toxicity of individual proteins, es ... | 1994 | 7854129 |
| experimental infection of aedes aegypti mosquitoes, suckling mice, and rats with four mosquito spiroplasmas. | four mosquito spiroplasmas, spiroplasma culicicola, spiroplasma sabaudiense, spiroplasma taiwanense, and an isolate designated cuas-1 were nonpathogenic for suckling mice and suckling rats inoculated either intracerebrally or intraperitoneally. s. culicicola persisted as long as 14 days in the brain of intracerebrally inoculated mice but did not replicate. the three other spiroplasmas were not reisolated from inoculated rats or mice. s. culicicola replicated in vitro at 37 degrees c in m1d media ... | 1994 | 7906284 |
| the suitability of restriction fragment length polymorphism markers for evaluating genetic diversity among and synteny between mosquito species. | restriction fragment length polymorphism (rflp) markers derived from the yellow fever mosquito, aedes aegypti, were used in hybridizations to genomic dna of the following mosquito species: ae. albopictus, ae. togoi, armigeres subalbatus, culex pipiens, and anopheles gambiae. interspecific hybridization with ae. aegypti probes varied from 50% (an. gambiae) to 100% (ae. albopictus) under high stringency conditions. we demonstrated the usefulness of using rflp profiles to examine genetic diversity ... | 1994 | 7909414 |
| adult cat flea (siphonaptera: pulicidae) excretion of host blood proteins in relation to larval nutrition. | protein content and ion composition of host blood and feces from blood-fed adult cat fleas, ctenocephalides felis (bouché), were examined. total fecal protein differed slightly from host blood indicating that there was little digestion of host blood by adult fleas. sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels of host blood and flea feces also revealed little digestion, as evidenced by the quantitatively and qualitatively similar protein banding patterns. blood and fecal ion com ... | 1994 | 7910638 |
| efficacy of clostridium bifermentans serovar malaysia on target and nontarget organisms. | clostridium bifermentans serovar malaysia (c.b.m.) is highly toxic to mosquito larvae. in this study, the following aquatic nontarget invertebrates were treated with high c.b.m. concentrations (up to 1,600-fold the toxic concentration for anopheles stephensi) to study their susceptibility towards the bacterial toxin: planorbis planorbis (pulmonata); asellus aquaticus (isopoda); daphnia pulex (cladocera); cloeon dipterum (ephemeroptera); plea leachi (heteroptera); and eristalis sp., chaoborus cry ... | 1994 | 7912261 |
| host range tests with edhazardia aedis (microsporida: culicosporidae) against northern nearctic mosquitoes. | twelve species of northern nearctic mosquitoes representing five genera (aedes, anopheles, culex, culiseta, and psorphora) were bioassayed for susceptibility to edhazardia aedis, an exotic, heterosporous, microsporidian parasite of the yellow fever mosquito, aedes aegypti. infections were achieved in aedes atropalpus, aedes triseriatus, and aedes vexans following oral ingestion of uninucleate spores obtained from patently infected ae. aegypti larvae. the microsporidium underwent normal vegetativ ... | 1994 | 7914904 |
| biochemical basis of ddt-resistance in aedes aegypti population from a dengue affected area in shahjahanpur city. | entomological studies conducted in jalalnagar, shahjahanpur city, uttar pradesh, india, during an outbreak of dengue in 1992, showed that ae. aegypti mosquitoes were resistant to ddt and had some tolerance to malathion in the adults and the larvae. biochemical analysis suggested that ddt resistance was related to elevated glutathione s-transferase and tolerance to malathion was due to a little increase in esterase activity. crosses of ddt-resistant and susceptible strain suggested that resistanc ... | 1994 | 7927578 |
| reinterpretation of the genetics of susceptibility of aedes aegypti to plasmodium gallinaceum. | several studies have demonstrated a genetic basis for variation in susceptibility of aedes aegypti to plasmodium gallinaceum. although 25 yr ago it was reported that p. gallinaceum susceptibility in ae. aegypti is determined primarily by a single autosomal dominant gene, evidence for additional genetic factors has emerged. two sublines, 1 refractory and 1 of intermediate susceptibility to p. gallinaceum, have been selected from the moyo-in-dry strain (moyo) of ae. aegypti. prior to selection, th ... | 1994 | 7931905 |
| reproductive fitness and survivorship of aedes aegypti (diptera: culicidae) maintained on blood, with field observations from thailand. | daily survivorship and fecundity of aedes aegypti mosquitoes were monitored for cohorts provided with five nutritional treatments: water, 2% sucrose, blood from a live chicken plus water, blood plus 2% sucrose, and blood alone. the median mortality time (lt50) for these females was 6, 54, 16, 12, and 29 d, respectively. there was no significant difference in the number of eggs laid by females in any of the treatments containing a host. females maintained on blood alone laid as many or more eggs ... | 1994 | 7932609 |
| mosquito gonotrophic cycle and multiple feeding potential: contrasts between anopheles and aedes (diptera: culicidae). | two aedine and three anopheline species were allowed to blood-feed to repletion, and their host-seeking behavior was measured with an olfactometer at intervals afterward. both aedes aegypti (l.) and ae. albopictus (skuse) were inhibited from subsequently seeking a host during the gonotrophic cycle, but anopheles gambiae giles, an. albimanus wiedemann, and an. freeborni aitken, all showed varying degrees of host-seeking behavior while their eggs matured. continued host-seeking by some anophelines ... | 1994 | 7932610 |
| inheritance of oral susceptibility of aedes aegypti to chikungunya virus. | a colony of rosy eye mutants of aedes aegypti was established. this strain was refractory to chikungunya virus by oral route of infection when compared with the wild-type parent strain. the refractoriness of this strain seems to be due to a mesentronal barrier, since both the mosquito strains supported the multiplication of virus after intrathoracic inoculation. the rosy eye strain was also found to be refractory to sagiyama virus (alphaviridae: getah virus subtype) when compared with wild-type ... | 1994 | 7943547 |
| microscopic visualization of wuchereria and brugia larval stages in intact cleared mosquitoes. | over the past several decades, epidemiologic data from filarial vectors typically has been obtained by mass dissection or by dissection of individual specimens. the former is quick and easy to do on large numbers of insects but provides no information on the frequency distribution of infection, presence of early developmental stages, or larval location; the latter is labor-intensive and tedious. we describe a new technique that can provide data comparable to those obtained by individual dissecti ... | 1994 | 7943576 |
| aedes aegypti: characterization of a hemolymph polypeptide expressed during melanotic encapsulation of filarial worms. | we report on the initial characterization of an 84-kda polypeptide that is differentially expressed in aedes aegypti during melanotic encapsulation immune reactions against filarial worms. [35s]methionine-labeled hemolymph from mosquitoes inoculated with saline, parasites that are melanized, or parasites that are not melanized was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. results show that the level of the 84-kda polypeptide increases considerably in those mosquitoes ... | 1994 | 7957753 |
| densovirus of aedes aegypti as an expression vector in mosquito cells. | we have constructed an infectious dna clone containing the genome of aedes aegypti densovirus (aednv) in a bacterial plasmid. when this clone was transfected into aedes albopictus c6/36 mosquito cells, the aednv genome rescued from the plasmid and replicated as the wild-type virus. to investigate the cloned virus as an expression vector, the reporter gene encoding beta-galactosidase (beta-gal) was inserted into four large open reading frames (orf) observed in the aednv genome. when these recombi ... | 1994 | 7957754 |
| insecticide susceptibility in mosquitoes (diptera: culicidae) from french polynesia. | susceptibility to six organophosphate (op), two pyrethroid (py), and one carbamate (c) insecticides was investigated in culex pipiens quinquefasciatus say, aedes aegypti (l.), and aedes polynesiensis marks larvae from the island of tahiti. cx. p. quinquefasciatus and ae. aegypti were compared with susceptible reference strains treated simultaneously. a low, but significant, resistance to bromophos (4.6x), chlorpyrifos (5.7x), fenthion (2.4x), fenitrothion (5.0x), temephos (4.3x) and permethrin ( ... | 1994 | 7966164 |
| salivary vasodilators of aedes triseriatus and anopheles gambiae (diptera: culicidae). | salivary vasodilators of aedes aegypti (l.) and anopheles albimanus (wiedemann) were characterized previously as a tachykinin peptide and a catechol oxidase/peroxidase activity, respectively. to verify whether these two different vasodilators also were found in other distantly related members of each mosquito genus, we characterized the vasodilators from a. triseriatus and a. gambiae. a. triseriatus salivary gland homogenates produced a reversible, endothelium dependent vasorelaxation of rabbit ... | 1994 | 7966179 |
| [seasonal variations in aedes aegypti populations in monterrey, mexico]. | seasonal variation of the populations of the dengue vector mosquito aedes aegypti, in the city of monterrey, n.l., mexico, showed a bimodal pattern. the first peak is lower and appeared at early june, while the second and higher one was observed in the second week of october. 1,419 female mosquitoes were caught in this study. females with fresh red blood in the stomach were present in all the catches, gravid females (mature eggs in the ovaries) being more abundant than the non-gravid ones. the g ... | 1994 | 7973991 |
| elisa for detecting dengue and japanese encephalitis viral antigen in mosquitoes. | an indirect elisa has been developed for detecting two viruses in triturated, experimentally infected mosquitoes: dengue (den) in aedes aegypti (l.) and ae. albopictus (skuse) and japanese encephalitis (je) viral antigen in culex tritaeniorhynchus giles. den antigen from four strains of the virus, representing each of the four serotypes, and je antigen were captured with a polyclonal anti-flavivirus igg and detected with a monoclonal antibody (4g2) that reacts with all flaviviruses. minimum vira ... | 1994 | 7979627 |
| differential modulation of murine cellular immune responses by salivary gland extract of aedes aegypti. | the ability of salivary gland extract (sge) of aedes aegypti to modulate cellular immune responses was investigated in a mouse model. cytokine production was induced in naive and antigen-primed murine (balb/c) spleen cells in vitro by stimulation with the t cell mitogen concanavalin a or the t cell-dependent antigen ovalbumin (ova), respectively. inclusion of ae. aegypti sge in in vitro culture with naive cells caused significant suppression of the cytokines interleukin-2 (il-2) and interferon g ... | 1994 | 7985763 |
| chromosomal mapping of two loci affecting filarial worm susceptibility in aedes aegypti. | two quantitative trait loci (qtl) affecting susceptibility of the mosquito aedes aegypti to the filarial worm parasite brugia malayi were identified using restriction fragment length polymorphism (rflp) markers. the first locus, fsb[1,lf178], resides within a 10 cm interval on chromosome 1 and exhibits a recessive effect with respect to susceptibility. the second locus, fsb[2,lf98], resides within a 9 cm interval on chromosome 2 and exhibits an additive effect on susceptibility. significant epis ... | 1994 | 7987523 |
| location of a lepidopteran specificity region in insecticidal crystal protein cryiia from bacillus thuringiensis. | the bacillus thuringiensis insecticidal crystal protein cryiia has both high mosquito activity and gypsy moth activity; in contrast cryiib, which is 87% homologous, displays no mosquito activity and has a three-fold lower gypsy moth activity. the regions responsible for specificity against gypsy moth (lymantria dispar) and mosquito (aedes aegypti) larvae were located by introducing mlui and xhoi sites into homologous positions within the putative domain ii of both cryiia and cryiib genes, which ... | 1994 | 7997170 |
| comparative study of hemolymph phenoloxidase activity in aedes aegypti and anopheles quadrimaculatus and its role in encapsulation of brugia malayi microfilariae. | hemolymph phenoloxidase activity of sugar-fed and blood-fed females of anopheles quadrimaculatus and aedes aegypti showed similar characteristics. phenoloxidase was present as an inactive proenzyme in both mosquito species and was partially activated during collection of the hemolymph. in both mosquito species, phenoloxidase activity was modulated by different buffers and activated phenoloxidase did not need ca2+. enzymatic activity was higher in the hemocytes than in the plasma in both mosquito ... | 1994 | 7828033 |
| larvicidal microcystin toxins of cyanobacteria affect midgut epithelial cells of aedes aegypti mosquitoes. | 1994 | 7841498 | |
| dengue vector control: present status and future prospects. | dengue fever (df) and dengue haemorrhagic fever (dhf) have been the most common urban diseases in southeast asia since the 1950s. more recently, the diseases have spread to central and south america and are now considered as worldwide diseases. both aedes aegypti and aedes albopictus are involved in the transmission of df/dhf in southeast asian region. the paper discusses the present status and future prospects of aedes control with reference to the malaysian experience. vector control approache ... | 1994 | 7844836 |
| dengue in the health transition. | the "health transition" describes the medical consequences which accompany the demographic transition and development. in many asian countries, as the infectious diseases of infancy decline, such as diarrhea, acute respiratory disease, measles and malaria, so too, do infant mortality rates. as a consequence of falling infant mortality rates and declines in fertility, the age pyramid has become more rectangular. no longer is nearly half of the population under the age of 15 years. diseases of adu ... | 1994 | 7844846 |
| dengue vector control in the urban environment of taiwan. | an epidemic of dengue occurred in southern taiwan in 1987, and by the end of 1988 there were 10,420 reported cases, of which 60.5% were from kaohsiung municipality. the urbanization and industrialization of the municipality have resulted in a vast increase in the breeding habitats of the dengue vector, aedes aegypti. in the early stage of the epidemic, only space spraying with insecticides was undertaken, but the aedes density gradually increased and reached its peak in the third quarter of 1988 ... | 1994 | 7844847 |
| control of dengue vectors in singapore. | singapore has a well-established system for the surveillance, research and control of aedes aegypti and aedes albopictus. control of these vectors comes under the jurisdiction of the environmental public health division of the ministry of the environment. the strategy for aedes control is an integrated approach incorporating case detection, source reduction, health education and law enforcement. this is done through the quarantine & epidemiology department (dengue surveillance and research), vec ... | 1994 | 7844848 |
| intersectoral approaches to dengue vector control. | medical entomology in the context of urban vector control, especially for dengue, can be likened to the tail of a dog. vertically structured aedes aegypti campaigns such as run by gorgas and soper earlier this century relied on sufficient legislative backing for vector control to ensure that the tail was capable of wagging the dog. under these conditions, especially where individual rights do not intrude, vertical programs will be successful. the global expansion of dengue, dengue hemorrhagic fe ... | 1994 | 7844851 |
| ecology and control of dengue vector mosquitoes in taiwan. | due to rapid urbanization, industrialization and social changes in recent years, the use of packing materials and tires has dramatically increased in the taiwan area. what is more is that some parts of southern taiwan are short of water resources and water preservation with huge containers becomes part of custom in those areas. storage water containers, waste vessels and tires are good habitats for aedes. meanwhile, some persons traveling to dengue endemic countries bring the dengue disease back ... | 1994 | 7844855 |
| surveillance and control of aedes aegypti in epidemic areas of taiwan. | aedes aegypti is the main, if not the only, vector of dengue fever in taiwan. the dengue epidemics that have occurred in taiwan correlate with the distribution of aedes aegypti which is limited to south of the tropic of cancer. during the 1987 outbreak of dengue fever in taiwan, the average larval density for the months july-december in the five cities and counties of southern taiwan was 2,284 larvae per 100 households. after control measures were taken, the average annual larval density in the ... | 1994 | 7844856 |
| [aedes albopictus and aedes aegypti at ile de la réunion]. | aedes albopictus and aedes aegypti are both found on the island. the former, being the probable vector of dengue on the island, is highly anthropophilic. it breeds in domestic environments as well as in natural breeding sites. the latter has a more restricted distribution and is never anthropophilic. larvae of ae. aegypti are only found in natural habitats, always associated with ae. albopictus, but in small proportions. such an ecology and behaviour for the typicus form of ae. aegypti are abnor ... | 1994 | 7726666 |
| antibacterial peptides in insect vectors of tropical parasitic disease. | the induction and characterization of immune peptides in two groups of medically important insects, the mosquitoes and blackflies, is currently an important research area. mosquitoes transmit a variety of viral and parasitic diseases including yellow fever, dengue, malaria and lymphatic filariasis. simuliid black flies are vectors of river blindness. the diseases are together responsible for death and morbidity in millions of people each year. the relationship between inducible peptides and bact ... | 1994 | 7768149 |
| effect of sublethal dosages of insecticides on chikungunya virus susceptible and refractory strains of aedes aegypti. | three strains of aedes aegypti mosquitos viz (i) crs, refractory to chikungunya (chik) virus by oral route of infection but susceptible to ddt (2) css, susceptible to chik virus and also susceptible to ddt (3) css-ddtr, susceptible to chik virus but resistant to ddt, were examined for the effect of sublethal dosages of ddt and deltamethrin on their fecundity. biochemical analysis showed that there was an increase in glutathione s-transferase activity in the css-ddtr strain which was associated w ... | 1994 | 7777922 |
| breeding of aedes aegypti (l.) and aedes albopictus (skuse) in urban housing of sibu town, sarawak. | an aedes survey using various larval survey methods was conducted in 12 urban housing areas and 29 vacant lands in sibu town proper. aedes albopictus larvae were found in all areas surveyed while aedes aegypti larvae were present in 10 localities and 4 vacant lands. there were no significant difference in the house index, breteau and larval density index of these two aedes (stegomyia) species from the survey areas. the proportion of containers positive with ae. aegypti and ae. albopictus in area ... | 1994 | 7777923 |
| evaluation of the laboratory mouse model for screening topical mosquito repellents. | eight commercial repellents were tested against aedes aegypti 0 and 4 h after application in serial dilution to volunteers and laboratory mice. results were analyzed by multiple regression of percentage of biting (probit scale) on dose (logarithmic scale) and time. empirical correction terms for conversion of values obtained in tests on mice to values expected in tests on human volunteers were calculated from data obtained on 4 repellents and evaluated with data obtained on 4 others. corrected v ... | 1994 | 7707065 |
| aedes albopictus in muzquiz city, coahuila, mexico. | aedes albopictus was found in m. muzquiz, coahuila, mexico, in the summer of 1993. aedes albopictus larvae were collected in tires and bottles at low density. aedes aegypti, toxorhynchites theobaldi, culex quinquefasciatus, and culiseta incidens were collected in the same containers. | 1994 | 7707068 |
| population interaction of toxorhynchites splendens and aedes aegypti (diptera: culicidae) in the laboratory. | population interaction of toxorhynchites splendens and aedes aegypti in relation to the complexity of the breeding habitats and their initial number was studied in the laboratory. the predator and the prey were introduced in different ratios in the colony cages (1 m3) with different oviposition structures. predator-prey interaction lasted for 5-9 weeks without structural complexity of the oviposition containers. when there was a structural complexity, their interaction lasted for 18 weeks. durin ... | 1994 | 7667726 |
| larvicidal potential of five philippine plants against aedes aegypti (linnaeus) and culex quinquefasciatus (say). | five species of philippine plants, reported in the literature to have insecticidal properties, were selected for investigation, namely: anona squamosa ("atis" or sugar apple), eucalyptus globulus ("bagras" or olive gum eucalyptus), lansium domesticum ("lansones"), azadirachta indica ("neem") and codiaeum variegatum ("san francisco" or croton). these were screened and assayed for their larvicidal potential against aedes aedes aegypti (linnaeus) and culex quinquefasciatus (say) by exposing 3rd-4th ... | 1994 | 7667727 |
| dietary control of late trypsin gene transcription in aedes aegypti. | in aedes aegypti the levels of midgut trypsin activity after feeding are directly proportional to the protein concentration in the meal. the mechanisms of this up-regulatory event were investigated by analyzing the expression of the late trypsin gene under different dietary conditions. transcription of the gene was dependent on both the quality and quantity of protein in the meal. as measured by northern blot analysis, the levels of late trypsin gene expression increased up to 100-fold 24 h afte ... | 1994 | 7519098 |
| an efficient and easy method of infection of mosquito larvae from virus-contaminated cell cultures. | a new method for efficient infection of aedes aegypti larvae by the aedes albopictus densovirus, aapv is described. it consists of placing first or third instar larvae in culture flasks containing a chronically infected mosquito cell line. after 24 or 48 h of exposure to the contaminated culture, the larvae acquired the virus by feeding on infected cells. using this technique, up to 95% of first instar ae. aegypti larvae were found infected by the aapv. | 1994 | 7822456 |
| seasonality, prevalence and pathogenicity of the gregarine ascogregarina taiwanensis (apicomplexa: lecudinidae) in mosquitoes from florida. | aedes albopictus larvae collected in gainesville, fl, were infected with the gregarine ascogregarina taiwanensis. natural prevalence varied from 68 to 100%. eight mosquito species were tested in the laboratory for susceptibility to a. taiwanensis isolated from field-collected ae. albopictus. aedes aegypti, aedes albopictus, and aedes taeniorhynchus became 100% infected in the larval stage, whereas aedes triseriatus was less susceptible; culex quinquefasciatus, culex nigripalpus, culex territans, ... | 1994 | 7807086 |
| studies on bacillus thuringiensis strains isolated from swedish soils: insect toxicity and production of b. cereus-diarrhoeal-type enterotoxin. | at moderate concentration, 23 of 40 strains of bacillus thuringiensis isolated from sweden were toxic to trichoplusia ni and five were toxic to aedes aegypti. five of the strains were toxic to diabrotica undecimpunctata at high concentration, two were toxic to heliothis virescens at low concentration and five produced thuringiensin (formerly called β-exotoxin). no strain was toxic towards the beet armyworm spodoptera exigua at low concentration. twenty-three of the strains produced a b. cereus-d ... | 1994 | 24421086 |
| parasitism of aedes aegypti and ae. albopictus (diptera: culicidae) by ascogregarina spp. (apicomplexa: lecudinidae) in florida. | aedes aegypti (l.) and ae. albopictus (skuse) from 40 sites in 17 counties of florida were surveyed for gregarine parasites during the spring and autumn of 1993 and in july 1994. larvae collected from containers (mainly tires) were dissected to determine the number of gregarine trophozoites present. ascogregarina spp. were found at 70% of the sites and occurred as frequently in ae. aegypti populations as in ae. albopictus. within sites, parasite distributions were highly variable and aggregated ... | 1995 | 8551508 |
| unique specificity of in vitro inhibition of mosquito midgut trypsin-like activity correlates with in vivo inhibition of malaria parasite infectivity. | synchrony in the egress of plasmodium ookinetes from the food bolus and enzymatic digestion of the blood meal in the mosquito midgut suggests that digestive enzymes play a role in the successful transmission of malaria parasites. previously, we found that parasite-produced chitinase is essential for parasite transmission and can be activated by mosquito midgut protease. to determine the suitability of developing a transmission-blocking vaccine directed against mosquito trypsin-like enzyme(s), ae ... | 1995 | 7534722 |
| hemocyte alterations during melanotic encapsulation of brugia malayi in the mosquito armigeres subalbatus. | the involvement of hemocytes in melanotic encapsulation reactions against brugia malayi was assessed in armigeres subalbatus. hemocyte populations, epitope changes, phenol oxidase (po) activity, and the presence of an 84-kda polypeptide were investigated in mosquitoes exposed to a b. malayi-infective bloodmeal (= immune-activated), in mosquitoes given a noninfective bloodmeal (= controls), in nonbloodfed mosquitoes (= naive), or in some combination of these. total hemocyte populations in immune- ... | 1995 | 7535848 |
| analysis of a vitellogenin gene of the mosquito, aedes aegypti and comparisons to vitellogenins from other organisms. | a genomic clone of the aedes aegypti vitellogenin a1 gene was sequenced including 2015 bp of 5' untranscribed sequence, 6369 bp of open reading frame interrupted by two introns, and a short 3' untranslated region. primer extension was used to identify the transcription initiation site. the amino termini of the large and small subunits were located by n-terminal sequencing of vitellin purified from eggs. the length of the signal sequence and the position of the cleavage site between the two subun ... | 1995 | 7550249 |
| mosquito sensitivity to a scorpion neurotoxin expressed using an infectious sindbis virus vector. | the scorpion, androctonus australis hector, produces an insect-specific toxin (aahit) encoded by the scotox gene. to assess the toxicity of aahit for mosquitoes, we have taken a novel approach to express the scotox gene in vivo. we have engineered a double subgenomic sindbis (dssin) virus that contains the scotox gene in the viral genome and intrathoracically inoculated the virus (te/3'2j/scotox) into mosquitoes (aedes aegypti, ae. triseriatus and culex pipiens), houseflies (musca domestica) and ... | 1995 | 7551198 |
| venezuelan equine encephalitis--colombia, 1995. | an outbreak of venezuelan equine encephalitis (vee) that began in northwestern venezuela in april 1995 has spread westward to the guajira peninsula and to colombia (figure 1), resulting in an estimated minimum of 13,000 cases in humans and an undetermined number of equine deaths. governments of both countries have initiated efforts to control the spread of this outbreak by quarantining and vaccinating equines and applying insecticides. this report summarizes the ongoing investigation of the outb ... | 1995 | 7565549 |
| full sequence and characterization of two insect defensins: immune peptides from the mosquito aedes aegypti. | we report the complete amino acid sequence and biological activity of two immune peptides, from the yellow fever mosquito aedes aegypti, that are induced in response to infection. both peptides display biological activity against the gram positive microbe micrococcus luteus and substantial sequence homology to insect defensins, small heat-stable, antibiotic peptides previously described from several non-vector insects. these mosquito peptides, designated ae. aegypti defensins a and b, are isofor ... | 1995 | 7568275 |
| aminopropeptide of human procollagen type i: a marker for the identification of blood from children in the mosquito blood meal. | a capture enzyme-linked immunosorbent assay (elisa) to distinguish between blood from children and adults in the mosquito blood meal was examined using the alpha 1 chain of the aminopropeptide of human procollagen type i (pinp) as antigenic marker. rabbit anti-human pinp (alpha 1) antibody was used as catching antibody, and either normal serum from 288 african and 58 caucasian children and adults, or blood meals from 93 fed aedes aegypti, were examined. pinp in excess of 40 optical density units ... | 1995 | 7570897 |
| post-inoculation changes in enzyme activity of aedes aegypti infected with chikungunya virus. | levels of acetylcholinesterase, non-specific esterases, glutathione-s-transferase and glucose-6-phosphate dehydrogenase in aedes aegypti (l.) mosquitoes inoculated intrathoracally with chikungunya virus were elevated, as compared to uninoculated control insects. a number of these enzymes are important in the insects defence mechanism against xenobiotics, such as pesticides. malathion bioassays indicated a reduction in the susceptibility of experimentally injected insects with virus or virus-free ... | 1995 | 7572467 |
| laboratory evaluation of a new repellent camouflage face paint. | a combined formulation of the u.s. army's camouflage face paints and the new extended duration topical insect/arthropod repellent was tested on human volunteers to determine repellency and duration of protection (2-12 h) against aedes aegypti and anopheles stephensi. under laboratory conditions (27 degrees c and 80% rh), the face paint/repellent formulation provided > or = 95% repellency or better for up to 6 h for ae. aegypti and 8 h for an. stephensi. an analysis of variance showed no signific ... | 1995 | 7595441 |
| impact of deltamethrin-impregnated bednets on biting rates of mosquitoes in zaire. | in a rural area of zaire, the whole population of a village was protected by deltamethrin-impregnated mosquitoes bednets. a similar village was observed as a control. biting rates for mosquitoes were recorded in both villages. the principal man-biting species were mansonia africana, mansonia uniformis, and aedes aegypti. in the village protected by the impregnated mosquito bednets, the number of mansonia bites was reduced 96% indoors and at a lesser rate outdoors. biting rates of ae. aegypti dro ... | 1995 | 7595444 |
| aedes albopictus and other aedes (stegomyia) species in fiji. | during an assessment of the dengue situation in fiji in early 1992, a house-to-house survey of container-breeding aedes was made. discarded tires and water drums were identified as key breeding sites for the 4 potential dengue vectors: aedes aegypti, aedes albopictus, aedes pseudoscutellaris, and aedes polynesiensis. aedes albopictus were detected on viti levu, vanua levu, and on taveuni. examination of early records and of used tire importation suggests entry into fiji after july 1985 but well ... | 1995 | 7595450 |
| risk factors for dengue infection during an outbreak in yanes, puerto rico in 1991. | in november 1991, during a five-month dengue outbreak, we performed epidemiologic and serologic surveys linked to an earlier entomologic study in a community of 425 houses in yanes (florida), puerto rico. we obtained a household response rate of 95% (98 of 103) and blood samples from 84% (345 of 410) of the participants. dengue incidence, as volunteered by the respondents, was 5% (21 of 410), but serologic diagnosis (immunoglobulin m and igg-enzyme-linked immunosorbent assays [elisa]) indicated ... | 1995 | 7611553 |
| dna sequence and site of mutation of the gaba receptor of cyclodiene-resistant red flour beetle, tribolium castaneum. | using polymerase chain reactions (pcr) on cdna, the dna sequence of a membrane spanning region of a gaba receptor of the red flour beetle, tribolium castaneum was identified. the deduced amino acid sequence indicates that its basic structure is similar to the gaba receptor of rdl type subunits of drosophila melanogaster and of blattella germanica. particularly conserved are m1, m2 and m3 segments. within this 146 amino acid stretch, the gaba receptor from the red flour beetle differed from corre ... | 1995 | 7613764 |
| insect repellent formulations of n,n-diethyl-m-toluamide (deet) in a liposphere system: efficacy and skin uptake. | novel formulations for a deet in liposphere microdispersion in the form of lotion were prepared from natural solid triglycerides and phospholipids dispersed in buffer solution. the formulations containing 6.5, 10, and 20% deet were effective as a repellent against the common aggressive biting mosquitoes, aedes aegypti and anopheles stephensi, for up to 6 h. the acute dermal absorption of the 10% loaded formulation was conducted in rabbits using 14c-labeled deet. 14c-labeled deet, 10% in alcohol ... | 1995 | 7616186 |
| an immunological factor that affects anopheles gambiae survival. | high titers of antibodies against anopheles gambiae midguts were produced in new zealand rabbits to identify midgut targets for an antimosquito vaccine. the serum from one of 8 rabbits (designated r2b6) killed 71.6% (abbott's adjusted % mortality) of an. gambiae within 7 days. mosquitoes ingesting r2b6 serum were unable to absorb their blood meal nutrients, resulting in reduced oviposition and egg hatching rates. anopheles stephensi and anopheles arabiensis were also killed when ingesting r2b6 s ... | 1995 | 7616189 |
| field evaluation of alphacypermethrin and lambda-cyhalothrin against aedes aegypti and aedes albopictus in malaysia. | alphacypermethrin (fendona) and lambda-cyhalothrin (icon) were evaluated against sentinel bloodfed adults and 4th-instar larvae of aedes aegypti in a housing estate in malaysia. the impact of both pyrethroids on field populations of ae. aegypti and aedes albopictus were monitored weekly using oviposition trap samples. both alphacypermethrin and lambda-cyhalothrin showed adulticidal and larvicidal effects. | 1995 | 7616191 |
| mosquito vitellogenin receptor: purification, developmental and biochemical characterization. | vitellogenin receptors (vgrs) play a critical role in egg development of oviparous animals by mediating endocytosis of the major yolk protein precursor, vitellogenin. a modification of the method for extracting the mosquito (aedes aegypti) vgr from ovary membranes resulted in an 11-fold higher yield and 56-fold increase in relative purity of the vgr, in turn permitting purification, antibody production, and microsequencing. a kd of 15 nm was estimated from binding assays for the enriched vgr, in ... | 1995 | 7633468 |
| insect immunity: isolation of three novel inducible antibacterial defensins from the vector mosquito, aedes aegypti. | the injection of escherichia coli and micrococcus luteus into the hemocoel of aedes aegypti induces a potent antibacterial activity in the hemolymph. we have purified and fully characterized three 40-residue antibacterial peptides from the hemolymph of bacteria-challenged mosquitoes that are absent in naive mosquitoes. the peptides are potently active against gram-positive bacteria and against one of the gram-negative bacteria that were tested. the amino acid sequences clearly show that the thre ... | 1995 | 7633471 |
| dengue in french guiana, 1965-1993. | while it seems likely that dengue fever (df) has existed in french guiana for at least one century, data on outbreaks are sketchy before temporary eradication of the dengue vector mosquito aedes aegypti and its reestablishment in the early 1960s. dengue cases were serologically confirmed for the first time in 1965, and since then dengue epidemics have occurred at two to six year intervals, the most important occurring in 1968-1969, 1970, 1972, 1976, 1982, 1986, and 1992. three of the four dengue ... | 1995 | 7640693 |
| age and body size effects on blood meal size and multiple blood feeding by aedes aegypti (diptera: culicidae). | ten-day-old, parous aedes aegypti (l.) had a higher threshold of blood meal size for the inhibition of continued host-seeking than did 5 d-old, nulliparous females of the same size. older females of a smaller-bodied cohort had a higher threshold of blood meal size for the initiation of egg development than did younger females. in contrast, older larger-sized females had a lower blood meal-size threshold for egg development. the blood of paired avian hosts was marked with rubidium and cesium to s ... | 1995 | 7650708 |
| edhazardia aedis (microspora: culicosporidae) effects on the reproductive capacity of aedes aegypti (diptera: culicidae). | the influence of the microsporidium edhazardia aedis (kudo) on the survival and reproduction of its mosquito host, aedes aegypti (l.), was studied in the laboratory. survival, fecundity, egg hatch, and percentage of emergence for 4 gonotrophic cycles were compared for control and infected mosquitoes. control females oviposited an average of 123.1 eggs over 4 gonotrophic cycles, 86.1% of which hatched, whereas infected females laid an average of 38.0 eggs with a 69.3% hatch. emergence in progeny ... | 1995 | 7650718 |
| effects of two insect growth regulators on ecdysteroid production in aedes aegypti (diptera: culicidae). | diflubenzuron and oms 2017 are insect growth regulators that affect larval to adult development in aedes aegypti (l.) by altering ecdysis. when larvae were exposed to sublethal concentrations, surviving adults express reduced reproductive potential. in mosquitoes, ecdysteroids are important in larval and adult ovarian development. we applied 30% emergence reduction concentrations (ei30) of oms 2017 and diflubenzuron to 4th-instar ae. aegypti to determine if changes in ecdysteroid production may ... | 1995 | 7473612 |
| feeding the mosquito aedes aegypti with tmof and its analogs; effect on trypsin biosynthesis and egg development. | female aedes aegypti that were given a blood meal by enema deposited yolk in their oocytes and synthesized trypsinlike enzymes in their midgut. when females were given an enema of aea-tmof (trypsin modulating oostatic factor) (nh2-ydpapppppp-cooh) and blood both egg development and trypsin biosynthesis were inhibited. similar results were observed if tmof was mixed with the blood meal and fed to female mosquitoes through a membrane. renin inhibitor (nh2-phpfhffvyk-cooh) or poly proline given by ... | 1995 | 7480877 |
| aedes aegypti control in the lao people's democratic republic, with reference to copepods. | an evaluation of the lao aedes aegypti control program and of the predatory abilities of copepods from vientiane, lao people's democratic republic was undertaken before a field release of copepods in thongkankam village, vientiane. copepods were transported to australia for evaluation of predatory abilities and their survival under various nutrient and ph conditions. mesocyclops guangxiensis was chosen for release over m. aspericornis due to its higher reproduction rate and its ability to surviv ... | 1995 | 7485682 |
| a simulation model of the epidemiology of urban dengue fever: literature analysis, model development, preliminary validation, and samples of simulation results. | we have developed a pair of stochastic simulation models that describe the daily dynamics of dengue virus transmission in the urban environment. our goal has been to construct comprehensive models that take into account the majority of factors known to influence dengue epidemiology. the models have an orientation toward site-specific data and are designed to be used by operational programs as well as researchers. the first model, the container-inhabiting mosquito simulation model (cimsim), a wea ... | 1995 | 7485707 |
| isolation and identification of novel toxins from a new mosquitocidal isolate from malaysia, bacillus thuringiensis subsp. jegathesan. | a new mosquitocidal bacillus thuringiensis subsp., jegathesan, has recently been isolated from malaysia. parasporal crystal inclusions were purified from this strain and bioassayed against fourth-instar larvae of culex quinquefasciatus, aedes aegypti, aedes togoi, aedes albopictus, anopheles maculatus, and mansonia uniformis. the 50% lethal concentration of crystal inclusions for each species was 0.34, 8.08, 0.34, 17.59, 3.91, and 120 ng/ml, respectively. these values show that parasporal inclus ... | 1995 | 7487029 |
| two genes encoding midgut-specific maltase-like polypeptides from anopheles gambiae. | full-length cdna clones of two genes have been isolated from the african malaria vector mosquito, anopheles gambiae. these genes, designated agm1 and agm2, encode maltase-like polypeptides of 498 and 599 residues, respectively. deduced amino acid sequences contain a putative signal peptide sequence and four potential glycosylation sites. agm1 and agm2 show highest similarities to the mal1 gene from aedes aegypti and three clustered maltase genes from drosophila melanogaster. both genes are locat ... | 1995 | 7498424 |
| plasmodium gallinaceum: sporozoite invasion of aedes aegypti salivary glands is inhibited by anti-gland antibodies and by lectins. | there is evidence which suggests that malaria sporozoites recognize mosquito salivary glands by specific receptor-ligand interactions. we are interested in identifying the putative salivary gland receptor(s) for sporozoite invasion. we used an in vivo bioassay for sporozoite invasion of salivary glands. in this assay, purified sporozoites from mature oocytes of plasmodium gallinaceum were injected into aedes aegypti mosquitoes and salivary glands were dissected at different time points after inj ... | 1995 | 7498430 |
| aedes aegypti: a quantitative trait locus (qtl) influencing filarial worm intensity is linked to qtl for susceptibility to other mosquito-borne pathogens. | because intensity of infection was significantly increased in a substrain of aedes aegypti selected for susceptibility to the filarial worm, brugia malayi, experiments were designed to assess numbers of microfilariae (mf) ingested and midgut penetration by mf in this susceptible substrain as compared to a refractory substrain selected from the same parental stock. refractory mosquitoes ingested significantly fewer mf than susceptible mosquitoes and significantly fewer numbers of mf penetrated th ... | 1995 | 7498432 |
| plasmodium gallinaceum: mosquito peritrophic matrix and the parasite-vector compatibility. | transmission of malaria parasites occurs by relatively few species of mosquitoes. one proposed mechanism of refractoriness is an inability of certain plasmodium spp. to cross the peritrophic matrix (pm) in the midgut of an incompatible mosquito. we have tested this hypothesis by studying sporogonic development of plasmodium gallinaceum in susceptible (aedes aegypti and anopheles gambiae g3) and refractory (anopheles stephensi) mosquito species in the presence and absence of the pm. in the presen ... | 1995 | 7498435 |
| hirsutellin a, a toxic protein produced in vitro by hirsutella thompsonii. | a toxic protein, hirsutellin a, has been purified from the mite fungal pathogen, hirsutella thompsonii, using ammonium sulphate precipitation, ion exchange chromatography and gel filtration on bio-gel p-10. the protein has been characterized as a monomer with a molecular mass of 15 kda and an isoelectric point of 10.5. the amino acid composition and the n-terminal sequence of hirsutellin a (34 amino acids) have been determined. from these results, the toxin appears to be distinct from other know ... | 1995 | 7670635 |
| community participation in dengue prevention and control: a survey of knowledge, attitudes, and practice in trinidad and tobago. | the priorities of public health planners are often at variance with the community's own environmental sanitation priorities and perspectives. public opinion about individual, collective, and governmental responsibilities in addressing these issues and priorities is of particular importance when designing community-based programs. in a study conducted in trinidad and tobago on knowledge, attitudes, and practice regarding dengue, its prevention and control, a high level of awareness about dengue a ... | 1995 | 7677210 |
| the effect of multiple host contacts on the infectivity of dengue-2 virus-infected aedes aegypti. | this study was designed to determine if transmission rates for dengue-2 virus by aedes aegypti are altered by mosquitos probing a host for blood or imbibing blood prior to attempting transmission. aedes aegypti is known to contact multiple hosts during each egg-laying cycle and multiple host contacts might diminish the amount of virus in infected mosquito's salivary glands or ducts and render them functionally uninfective. probing a host 5, 10, or 20 consecutive times did not significantly alter ... | 1995 | 7707190 |
| mosquito bite anaphylaxis: immunotherapy with whole body extracts. | adverse reactions to mosquito bites have been recognized for some time. these usually consist of large local swellings and redness, generalized urticaria, angioedema and less easily definable responses such as nausea, dizziness, headaches, and lethargy. | 1995 | 7719881 |
| blood-feeding behavior of dengue-2 virus-infected aedes aegypti. | this study was designed to determine if infection of aedes aegypti with dengue-2 virus affects the ability of the mosquito to efficiently locate and imbibe blood from an uninfected host. previous studies suggest that some parasites manipulate their arthropod host to increase the probability of transmission by interfering with blood-feeding efficiency. we found no evidence that dengue-2 virus infection by intrathoracic inoculation impaired the blood-feeding efficiency of ae. aegypti. we speculate ... | 1995 | 7694963 |
| proportions of eggs laid by aedes aegypti on different substrates within an ovitrap in trinidad, west indies. | ovitraps were exposed outdoors during wet and dry seasons in trinidad to determine the proportions of eggs laid on three substrates: the widely-used hardboard 'paddle' (p), the water surface (w) and the inside surface of the ovitrap wall (s) in three types of blackened ovitrap: round, glass (rg), round, plastic (rp) and rectangular, plastic (sp). of the 3556 eggs obtained, most (86.4%) were laid during the wet season when statistically significant differences were detected between substrates but ... | 1995 | 7696690 |
| [deficiency in public services and breeding of aedes aegypti in venezuela]. | 1995 | 7786414 | |
| restriction fragment length polymorphism mapping of quantitative trait loci for malaria parasite susceptibility in the mosquito aedes aegypti. | susceptibility of the mosquito aedes aegypti to the malarial parasite plasmodium gallinaceum was investigated as a quantitative trait using restriction fragment length polymorphisms (rflp). two f2 populations of mosquitoes were independently prepared from pairwise matings between a highly susceptible and a refractory strain of a. aegypti. rflp were tested for association with oocyst development on the mosquito midgut. two putative quantitative trait loci (qtl) were identified that significantly ... | 1995 | 7789771 |
| brugia pahangi: the effects of cecropins on microfilariae in vitro and in aedes aegypti. | synthetic cecropins, antibacterial peptides from insect haemolymph, have been tested for their ability to attenuate the motility of microfilariae of the filarial nematode brugia pahangi in an in vitro assay. fifty micromolar concentrations of these peptides, equivalent to physiological concentrations in immune-stimulated insects, cause significant attenuation of motility compared with untreated microfilariae. similar results were obtained with cecropins a and b. this is the lowest concentration ... | 1995 | 7729475 |
| indirect control of yolk protein genes by 20-hydroxyecdysone in the fat body of the mosquito, aedes aegypti. | in response to a blood meal, the fat body of the female mosquito, aedes aegypti, begins massive production of several yolk proteins which are subsequently stored in the developing oocytes. although 20-hydroxyecdysone (20e) is important for initiation and maintenance of expression of the yolk protein genes encoding vitellogenin (vg) and vitellogenic carboxypeptidase (vcp), the exact nature of 20e action has not been clearly defined. a primary question is whether this hormone directly stimulates e ... | 1995 | 7742832 |
| comparison of migration and encapsulation of brugia malayi microfilariae from the midgut to the hemocoel between anopheles quadrimaculatus and aedes aegypti. | comparisons were made of migration and encapsulation of ingested sheathed microfilariae of brugia malayi from the midgut into the hemocoel between anopheles quadrimaculatus (refractory and susceptible strains to b. malayi) and aedes aegypti (black-eyed, liverpool strain susceptible to b. malayi). encapsulation and melanization of microfilarial sheaths and microfilariae occurred in both strains of an. quadrimaculatus and in ae. aegypti. in both strains of an. quadrimaculatus, by 4 hr and by 24 hr ... | 1995 | 7745283 |
| mosquito larvicidal activity of escherichia coli with combinations of genes from bacillus thuringiensis subsp. israelensis. | the genes cryiva and cryivd, encoding 134- and 72-kda proteins, respectively, and the gene for a regulatory 20-kda polypeptide of bacillus thuringiensis subsp. israelensis (serovar h14) were cloned in all seven possible combinations by the escherichia coli expression vectors pt7 and puhe. the four combinations containing cryiva (cryiva alone, with cryivd, with the 20-kda-protein gene, and with both) displayed high levels of mosquito larvicidal activity in puhe. the toxicity of the combination of ... | 1995 | 7751296 |
| dengue serologic survey in ribeirão preto, são paulo, brazil. | the city of ribeirão preto suffered a dengue 1 epidemic that began in november 1990 and ended in march 1991. a serologic survey designed to detect igg antibodies to the four dengue serotypes and other flaviviruses was carried out in ribeirão preto during september and october of 1992. dengue 1 antibodies were detected in 5.4% of the survey participants. significantly higher seropositivity (9.3%) was found among subjects residing in the northwest sector of ribeirão preto than among those living i ... | 1995 | 7757124 |
| isolation of antifungal and larvicidal constituents of diplolophium buchanani by centrifugal partition chromatography. | three phenylpropanoids, myristicin [1], elemicin [2], and trans-isoelemicin [3], together with two furanocoumarins, oxypeucedanin [4] and oxypeucedanin hydrate [5], have been isolated from the leaves of diplolophium buchanani by a separation strategy involving the almost exclusive use of centrifugal partition chromatography. all five compounds were antifungal in a tlc bioautography test using cladosporium cucumerinum. compounds 1-4 exhibited larvicidal activity against aedes aegypti. | 1995 | 7760070 |
| concomitant malaria (plasmodium gallinaceum) and filaria (brugia pahangi) infections in aedes aegypti: effect on parasite development. | mixed infections with malarial (plasmodium gallinaceum) and filarial (brugia pahangi) parasites were carried out in 8 trials with filaria susceptible (refm) and filaria refractory (rep-rr) aedes aegypti strains. a secondary infection with b. pahangi microfilariae (mff) by intrathoracic inoculation, reduced the development rate of a pre-existing p. gallinaceum infection. the level of reduction ranged from 9.5 to 49% in refm and from 50 to 90% in rep-rr. an immune response against oocysts was seen ... | 1995 | 7845706 |
| estimates of population size, dispersal, and longevity of domestic aedes aegypti aegypti (diptera: culicidae) by mark-release-recapture in the village of shauri moyo in eastern kenya. | estimates of the absolute size of a domestic population of aedes aegypti aegypti (l.) were made in the rabai area of kenya, based on a single release, followed by either single or repeated recaptures. from single recapture within 24 h of release, the size of the female ae. a. aegypti population in the shauri moyo village was estimated by the lincoln index to be 365. using a single release and repeated recaptures, population size was estimated by jackson's positive method to be 337. depletion of ... | 1995 | 7869339 |
| growth-restricted dengue virus mutants containing deletions in the 5' noncoding region of the rna genome. | the dengue type 4 virus (den4) rna genome contains a 101-nt 5' noncoding (nc) sequence which is predicted to form a stable secondary structure. den4 cdna from which infectious rna can be transcribed was used to engineer deletions in the 5' nc region for functional analysis of rna structure and for isolation of den4 mutants that could be evaluated as candidates for use in a live attenuated vaccine. eleven distinct deletions in the region of the den4 genome between nts 18 and 98 were constructed; ... | 1995 | 7871753 |
| short report: dispersal of aedes aegypti in an urban area after blood feeding as demonstrated by rubidium-marked eggs. | strategies for the control of aedes aegypti during urban outbreaks of dengue or yellow fever assume that this species has a maximum flight range of 50-100 meters. because ae. aegypti distributes its eggs among several oviposition sites, we postulated that dispersal is driven by the search for oviposition sites, so an ovipositing female may have to fly much further than 50-100 meters to lay all of her eggs. we developed a method for marking ae. aegypti eggs with a rare alkali metal (rubidium) and ... | 1995 | 7872449 |
| 5-hydroxytryptamine in the salivary glands of adult female aedes aegypti and its role in regulation of salivation. | a dense plexus of axons, immunoreactive to antisera against 5-hydroxytryptamine (5-ht, serotonin) and surrounding the proximal medial lobe of the salivary gland of adult female aedes aegypti mosquitoes, was demonstrated by means of whole-mount fluorescence immunocytochemistry. this innervation originates in the stomatogastric nervous system. 5-ht-immunoreactive innervation is absent in male salivary glands, suggesting that 5-ht is involved in blood-feeding. furthermore, female mosquitoes treated ... | 1995 | 7891033 |
| understanding productivity, a key to aedes aegypti surveillance. | the objective of this work was to define criteria that could be applied to achieve faster, more economical, and accurate assessment of vector populations for control of dengue viruses. during 1989-1990, 1,349 premises were surveyed in townsville, charters towers and mingela/ravenswood, queensland, australia. in each locality, 1.9-8.4% of premises contained three or more containers with aedes aegypti immature forms and were designated as key premises. comparison of surveys in townsville from 1989 ... | 1995 | 8561260 |
| infection of aedes albopictus (diptera: culicidae) and ae. aegypti with lambornella stegomyiae (ciliophora: tetrahymenidae). | the transformation of lambornella stegomyiae trophonts to theronts, the distribution of invasion cysts on larval aedes albopictus cuticle, and the virulence of l. stegomyiae to ae. albopictus and aedes aegypti were studied in the laboratory. transformation of trophonts into theronts was induced by a morphogenic agent released from larval ae. albopictus homogenate. the first transformation was observed 4 hr after exposure to larval mosquito homogenate, but most transformations occurred between 12 ... | 1995 | 8568285 |
| towards malaria control in nigeria: a qualitative study on the population of mosquitoes. | malaria is still highly prevalent in many tropical countries and this disease can only survive in areas where mosquitoes and infected human populations are high. relevant information on the species of mosquitoes, their habitats and their population are important in planning preventative strategies in the control of malaria, hence this study. mosquito species and their habitats were investigated in both high and low density areas of benin city, edo state, nigeria. the results showed that culex pi ... | 1995 | 8568784 |
| immunohistological localization of regulatory peptides in the midgut of the female mosquito aedes aegypti. | the midgut of the female mosquito aedes aegypti was studied immunohistologically with antisera to various regulatory peptides. endocrine cells immunoreactive with antisera to perisulfakinin, rfamide, bovine pancreatic polypeptide, urotensin 1, locustatachykinin 2 and allatostatins a1 and b2 were found in the midgut. perisulfakinin, rfamide and bovine pancreatic polypeptide all react with the same, about 500 endocrine cells, which were evenly distributed throughout the posterior midgut, with the ... | 1995 | 8574883 |
| jungle yellow fever: clinical and laboratorial studies emphasizing viremia on a human case. | the authors report the clinical, laboratorial and epidemiological aspects of a human case of jungle yellow fever. the patient suffered from fever, chills, sweating, headaches, backaches, myalgia, epigastric pains, nausea, vomiting, diarrhea and prostration. he was unvaccinated and had been working in areas where cases of jungle yellow fever had been confirmed. investigations concerning the yellow fever virus were performed. blood samples were collected on several days in the course of the illnes ... | 1995 | 8599063 |
| [yellow fever in mexico. the eradication of aëdes aegypti. 1966]. | 1995 | 8599142 |