Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| factors associated with nucleic acids related to human immunodeficiency virus type 1 in cervico-vaginal secretions. | to assess hiv-related nucleic acids in cervico-vaginal secretions and the factors associated with them. | 2001 | 11426900 |
| antimicrobial activity of cassia alata. | the methanol extracts of leaves, flowers, stem and root barks of cassia alata showed a broad spectrum of antibacterial activity. the activity was increased on fractionation (petrol, dichloromethane, ethyl acetate), the dichloromethane fraction of the flower extract being the most effective. no activity was shown against tested moulds. | 2001 | 11429256 |
| antimicrobial activity of clematis papuasica and nauclea obversifolia. | the methanol extracts of clematis papuasica leaves and stem bark and of nauclea obversifolia leaves, stem and root barks showed awide spectrum of antibacterial activity which was increased on fractionation (petrol, dichloromethane, ethyl acetate), the ethyl acetate fractions being in all cases the most effective. none of the extractives was active against tested moulds. | 2001 | 11429260 |
| ornidazole-induced autoimmune hepatitis. | ornidazole is a commonly prescribed antiparasitic drug for parasitic infestations, including amoebiasis, giardiasis and trichomonas vaginalis. several cases of antibiotic-induced autoimmune hepatitis (aih) or aih-like syndrome have been reported recently. in this report, we describe a 35-year-old woman with two relapses of aih induced by ornidazole prescribed for diarrhoea and vaginal infection. | 2001 | 11434605 |
| prevalence of metronidazole-resistant trichomonas vaginalis in a gynecology clinic. | to determine the prevalence of in vitro resistance to metronidazole among unselected isolates of trichomonas vaginalis and correlate in vitro findings with response to metronidazole therapy. | 2001 | 11441678 |
| methionine regeneration and aspartate aminotransferase in parasitic protozoa. | aspartate aminotransferases have been cloned and expressed from crithidia fasciculata, trypanosoma brucei brucei, giardia intestinalis, and plasmodium falciparum and have been found to play a role in the final step of methionine regeneration from methylthioadenosine. all five enzymes contain sequence motifs consistent with membership in the ia subfamily of aminotransferases; the crithidial and giardial enzymes and one trypanosomal enzyme were identified as cytoplasmic aspartate aminotransferases ... | 2001 | 11443076 |
| transcriptome meets metabolome: hierarchical and metabolic regulation of the glycolytic pathway. | the fact that information flows from dna to rna to protein to function suggests that regulation is 'hierarchical', i.e. dominated by regulation of gene expression. in the case of dominant regulation at the metabolic level, however, there is no quantitative relationship between mrna levels and function. we here develop a method to quantitate the relative contributions of metabolic and hierarchical regulation. applying this method to the glycolytic flux in three species of parasitic protists, we c ... | 2001 | 11445079 |
| sexually transmitted infections and reproductive health in azerbaijan. | little is known about the prevalence of sexually transmitted infections (stis) and about sexual and reproductive health in central and eastern europe. however, it is clear that major epidemics of stis currently exist. | 2001 | 11460020 |
| metronidazole-resistant strains of trichomonas vaginalis display increased susceptibility to oxygen. | susceptibility to oxygen and properties relative to oxygen metabolism were compared in metronidazole-resistant and susceptible strains of trichomonas vaginalis. the study involved clinical isolates displaying the aerobic type of resistance, as well as resistant strains developed in vitro, both with aerobic (mr-3) and anaerobic (mr-5, mr-100) resistance. elevated sensitivity to oxygen of the resistant clinical isolates was observed. progressive increase of susceptibility to oxygen also accompanie ... | 2001 | 11467782 |
| phylogenetic relationships of class ii fumarase genes from trichomonad species. | class ii fumarase sequences were obtained by polymerase chain reaction from five trichomonad species. all residues known to be highly conserved in this enzyme were present. nuclear run-on assays showed that one of the two genes identified in tritrichomonas foetus was expressed, whereas no fumarase transcripts were detected in the related species trichomonas vaginalis. these findings corroborate previous biochemical data. fumarase genes were also expressed in monocercomonas sp. and tetratrichomon ... | 2001 | 11470849 |
| validity of self-reported sexually transmitted diseases among african american female adolescents participating in an hiv/std prevention intervention trial. | studies assessing the validity attributed to self-reported measures of sexually transmitted diseases (stds) clearly are needed, particularly those used for high-risk populations such as female adolescents, in whom std prevention is a priority. | 2001 | 11473220 |
| immunolocalization of tubulin isoforms and post-translational modifications in the protists tritrichomonas foetus and trichomonas vaginalis. | in the present report we show the distribution of multiple tubulin isoforms in trichomonas vaginalis and tritrichomonas foetus, flagellated parasitic protists of the urogenital tracts of human and cattle, respectively, using immunofluorescence and immunoelectron microscopy. we used several monoclonal and polyclonal anti-tubulin antibodies from different sources and recognizing variant tubulin isoforms. our results demonstrate that: (1) there is a heterogeneous distribution of the different tubul ... | 2001 | 11479719 |
| subsequent sexually transmitted infection in urban adolescents and young adults. | to compare the rates of subsequent infection with chlamydia trachomatis, neisseria gonorrhoeae, or trichomonas vaginalis in a group of high-risk adolescents and young adults. | 2001 | 11483124 |
| midtrimester vaginal mycoplasma genitalium in women with subsequent spontaneous preterm birth. | we sought to determine the midtrimester prevalence of mycoplasma genitalium in women who had subsequent spontaneous preterm birth. | 2001 | 11483922 |
| evaluation of two self-care treatments for prevention of vaginal candidiasis in women with hiv. | vaginal candidiasis (vc) is a common concern for women living with hiv infection. the authors evaluated the effectiveness of two self-care approaches to prophylaxis of vc among hiv-infected women, weekly intravaginal application of lactobacillus acidophilus or weekly intravaginal application of clotrimazole tablets, in a randomized, double-blind, placebo-controlled trial. vc was defined as a vaginal swab positive for candida species in the presence of signs/symptoms of vaginitis and the absence ... | 2001 | 11486720 |
| sexually transmitted infections in estonia--syndromic management of urethritis in a european country? | sexually transmitted infections (stis) are considered a major public health problem, globally. in particular, increasing sti rates have been documented throughout eastern europe and central asia. the russian federation and adjacent countries have, traditionally, managed stis on an aetiological basis. this approach is expensive in terms of laboratory costs and it may lead to delayed diagnosis and treatment. to overcome the limitations of the aetiological management of stis, the world health organ ... | 2001 | 11487388 |
| binding of fibronectin by trichomonas vaginalis is influenced by iron and calcium. | we have reported that trichomonas vaginalis, the causative agent of the most common, non-viral sexually transmitted disease, bound to cover slips coated with fibronectin (fn) (crouch & alderete, microbiol 1999 145: 2835-43). in this study, we extend that observation by showing that fn binding is specific, and we present data on the requirements of fn binding by t. vaginalis. immunofluorescence and immuno-gold labelling readily detected fn throughout the trichomonal surface. parasites bound to(12 ... | 2001 | 11500098 |
| prevalence of sexually transmitted infections (neisseria gonorrhoeae, chlamydia trachomatis, trichomonas vaginalis and human papillomavirus) in female attendees of a sexually transmitted diseases clinic in ulaanbaatar, mongolia. | epidemiological data suggest that the prevalence of syphilis, gonorrhea and trichomoniasis has increased in both urban and rural areas of mongolia. these data are primarily substantiated by notifications of cases of clinically apparent disease in both rural and urban areas, plus laboratory diagnoses from the aids/std reference center, ulaanbaatar. in the past 5 years, however, there has been a marked decline in the total number of patients being screened for sexually transmitted infections (stis ... | 2001 | 11516062 |
| metronidazole treatment of women with a positive fetal fibronectin test result. | eighty-nine women with either bacterial vaginosis, trichomonas vaginalis, or both, who also had a positive fetal fibronectin test result were randomized to two courses of metronidazole treatment as part of a maternal-fetal medicine network units study of the national institute of child health and human development. in this subgroup analysis, compared with the placebo group, women who were treated with metronidazole had a nonsignificant reduction in spontaneous preterm birth from 14.6% to 8.3%. | 2001 | 11518914 |
| failure of metronidazole to prevent preterm delivery among pregnant women with asymptomatic trichomonas vaginalis infection. | infection with trichomonas vaginalis during pregnancy has been associated with preterm delivery. it is uncertain whether treatment of asymptomatic trichomoniasis in pregnant women reduces the occurrence of preterm delivery. | 2001 | 11519502 |
| evaluation of the affirm ambient temperature transport system for the detection and identification of trichomonas vaginalis, gardnerella vaginalis, and candida species from vaginal fluid specimens. | the objective of this study was to measure the performance of the affirm ambient temperature transport system (atts) over time and to estimate the length of time the system can preserve a vaginal specimen containing the three common organisms causing vaginitis: trichomonas vaginalis, candida species, and gardnerella vaginalis (one of the causative agents of bacterial vaginosis). women with symptoms of vaginitis presenting to one of three clinical centers were evaluated over a 4- to 8-week period ... | 2001 | 11526150 |
| correlation between urine and cervical specimens for the detection of cervical chlamydia trachomatis and neisseria gonorrhoeae using ligase chain reaction in a cohort of hiv infected and uninfected adolescents. | (a) to examine the concordance between ligase chain reaction (lcr) results from urine and cervical samples for chlamydia trachomatis and neisseria gonorrhoeae in hiv infected and uninfected adolescent women, and (b) to examine factors that may influence the concordance of lcr results in this population. | 2001 | 11530308 |
| anti-microbial activity of bidens pilosa, bischofia javanica, elmerillia papuana and sigesbekia orientalis. | the ethanol extracts of bidens pilosa (whole plant), bischofia javanica (leaves), elmerillia papuana (root bark) and sigesbekia orientalis (whole plant) were partitioned (petrol, dichloromethane, ethyl acetate). the crude ethanolic extracts and all the obtained fractions showed a broad spectrum of antibacterial activity, the ethyl acetate fractions and the petrol fraction of e. papuana being the most effective. no activity was observed against the tested moulds. | 2001 | 11543964 |
| antimicrobial activity of lithocarpus celebicus. | the methanol extracts of leaves, stem and root barks of lithocarpus celebicus showed a broad spectrum of antibacterial activity which was increased on fractionation (petrol, dichloromethane, ethylacetate), particularly in the ethyl acetate fraction of the stem bark and petrol fraction of the root bark. none of the extractives was active against tested moulds. | 2001 | 11543975 |
| mitochondrial type iron-sulfur cluster assembly in the amitochondriate eukaryotes trichomonas vaginalis and giardia intestinalis, as indicated by the phylogeny of iscs. | pyridoxal-5'-phosphate-dependent cysteine desulfurase (iscs) is an essential enzyme in the assembly of fes clusters in bacteria as well as in the mitochondria of eukaryotes. although fes proteins are particularly important for the energy metabolism of amitochondrial anaerobic eukaryotes, there is no information about fes cluster formation in these organisms. we identified and sequenced two iscs homologs of trichomonas vaginalis (tviscs-1 and tviscs-2) and one of giardia intestinalis (giiscs). tv ... | 2001 | 11557797 |
| tinidazole therapy for metronidazole-resistant vaginal trichomoniasis. | treatment of patients with metronidazole-refractory vaginal trichomoniasis constitutes a major therapeutic challenge, and treatment options are extremely limited. although the majority of patients infected with trichomonads, who demonstrate reduced in vitro susceptibility to metronidazole, respond to high-dose but poorly tolerated regimens of metronidazole, clinical failure is by no means uncommon. we report a cure rate of 22 (92%) of 24 patients with refractory trichomoniasis treated with high ... | 2001 | 11565074 |
| evaluation of use of a single intravaginal swab to detect multiple sexually transmitted infections in active-duty military women. | the accuracy and suitability of use of a single intravaginal swab (sis) for polymerase chain reaction detection of neisseria gonorrhoeae, chlamydia trachomatis, trichomonas vaginalis, and human papillomavirus infection was assessed in a cross-sectional study of 841 active-duty military women. the sis, compared with standard diagnostic tests, allowed detection of more gonorrhea, more chlamydial infection, and more trichomoniasis. sensitivity and specificity of sis detection compared with adjudica ... | 2001 | 11568849 |
| [spectrum of microflora isolated from various areas of the female reproductive tract]. | the composition of microflora in different sections of the reproductive tract of women with disturbances of reproductive function was studied. the study revealed that the spectrum of microorganisms isolated from a bioptic specimen taken from the cavity of the small pelvis was narrower in comparison with such spectrum in material aspirated from the uterine cavity. the latter was narrower in comparison with that in the microflora of the lower sections of the reproductive tract. the possibility for ... | 2001 | 11569275 |
| a historic photomicrograph of a parasite (trichomonas vaginalis). | knowing that alfred donné was the discoverer of an important human parasite, and finding that he was also a pioneer of photomicrography, it occurred to me that his parasite might well have become a subject of his photography. it was a simple matter to confirm that this was indeed the case. the parasite he discovered was trichomonas vaginalis; and, in collaboration with foucault, donné made a photomicrograph showing several protozoan parasites lying among vaginal epithelial cells. his publication ... | 2001 | 11587965 |
| [frequency of gardnerella vaginalis vaginosis and its association with other pathogens causing genital infections in the female]. | in order to determine the presence of unspecific vaginosis and their causes, 700 vaginal smears were obtained from patients assisting to the clinical laboratory of the familiar medicine unit no. 28 "gabriel mancera" of the instituto mexicano del seguro social, during 6 months. the patients age was from 18 to 55 years old. to establish the infectious etiology of these pathologies the vaginal smears were observed freshly and stained by gram's method. specific test for differentiate gardnerella vag ... | 2001 | 11588810 |
| [metronidazole]. | metronidazole was first introduced for the treatment of trichomoniasis. now, its therapeutics use has subsequently been expanded to include protozoal and anaerobic infections. oral administration is recommended: rosacea, perioral dermatitis, helicobacter pylori, trichomonas vaginalis and giardia lamblia infections and bacterial vaginosis. metronidazole given orally is absorbed almost completely. metronidazole has limited plasma protein binding but can reach very favourable tissue distribution, i ... | 2001 | 11590342 |
| initiator recognition in a primitive eukaryote: ibp39, an initiator-binding protein from trichomonas vaginalis. | while considerable progress has been made in understanding the mechanisms of transcription in higher eukaryotes, transcription in single-celled, primitive eukaryotes remains poorly understood. promoters of protein-encoding genes in the parasitic protist trichomonas vaginalis, which represents one of the deepest-branching eukaryotic lineages, have a bipartite structure with gene-specific regulatory elements and a conserved core promoter encompassing the transcription start site. core promoters in ... | 2001 | 11604521 |
| structure and division of the golgi complex in trichomonas vaginalis and tritrichomonas foetus. | we present observations on the fine structure and the division process of the golgi complex in the protists trichomonas vaginalis and tritrichomonas foetus, parasites of the urogenital tract of humans and cattle, respectively. the golgi in trichomonads is a prominent structure, associated with striated parabasal filaments to which this organelle seems to be connected. we followed by immunofluorescence and electron microscopy the golgi in interphasic and mitotic cells. ultrastructural studies wer ... | 2001 | 11675935 |
| taqman-based detection of trichomonas vaginalis dna from female genital specimens. | a double-labeled fluorescent probe was designed and evaluated for detecting trichomonas vaginalis dna in a 5' nuclease (taqman) assay. the t. vaginalis-specific probe contains a 5'-fluorescein (5'-fam) and a 3'-rhodamine (tamra) derivative. female genital secretions were collected on amplicor (roche molecular, indianapolis, ind.) swabs and by a transport system used for chlamydia trachomatis and/or neisseria gonorrhoeae dna detection by pcr. five hundred fifty-two female genital specimens, of wh ... | 2001 | 11682492 |
| ecological and individual level analysis of risk factors for hiv infection in four urban populations in sub-saharan africa with different levels of hiv infection. | to identify factors that could explain differences in rate of spread of hiv between different regions in sub-saharan africa. | 2001 | 11686462 |
| the epidemiology of gonorrhoea, chlamydial infection and syphilis in four african cities. | to compare the epidemiology of gonorrhoea, chlamydial infection and syphilis in four cities in sub-saharan africa; two with a high prevalence of hiv infection (kisumu, kenya and ndola, zambia), and two with a relatively low hiv prevalence (cotonou, benin and yaoundé, cameroon). | 2001 | 11686469 |
| the epidemiology of trichomoniasis in women in four african cities. | to describe the epidemiology of trichomonas vaginalis infection and its association with hiv infection, in women in four african cities with different levels of hiv infection. | 2001 | 11686470 |
| high rate of bacterial vaginosis among women with intrauterine devices in manado, indonesia. | recent research reported that bacterial vaginosis (bv) might enhance the acquisition and transmission of hiv. bv is also associated with an increased risk of pelvic inflammatory disease, a disease also associated with intrauterine device (iud) insertion. to measure the magnitude of this problem, we conducted a prevalence survey of bv and sexually transmitted diseases (stds; defined as current infections with neisseria gonorrhoeae, chlamydia trachomatis, and/or trichomonas vaginalis) among all pa ... | 2001 | 11704096 |
| randomized trial of presumptive sexually transmitted disease therapy during pregnancy in rakai, uganda. | the purpose of this study was to assess presumptive sexually transmitted disease treatment on pregnancy outcome and hiv transmission. | 2001 | 11717659 |
| nongonococcal and nonchlamydial cervicitis. | cervicitis encompasses a variety of gynecologic conditions. although inflammation on a papanicolaou smear may be indicative of true cervical inflammation, establishing a diagnosis of mucopurulent cervicitis (mcp) is more clinically useful. mcp seems to be analogous to nongonococcal urethritis in men. many cases of mcp are related to chlamydia trachomatis infection, but the majority of cases have other potential causes. other possible pathogens include neisseria gonorrhoeae, trichomonas vaginalis ... | 2001 | 11722812 |
| frequency of trichomonas vaginalis, candida sp and gardnerella vaginalis in cervical-vaginal smears in four different decades. | vaginitis is one of the principal motives that lead women to seek out an obstetrician or gynecologist. bacterial vaginosis, candidiasis and trichomoniasis are responsible for 90% of the cases of infectious vaginitis. | 2001 | 11723534 |
| phagocytosis of rod-shaped bacteria and cocci by trichomonas vaginalis: light microscopic observations. | 2001 | 11726111 | |
| concordance between genetic relatedness and phenotypic similarities of trichomonas vaginalis strains. | despite the medical importance of trichomoniasis, little is known about the genetic relatedness of trichomonas vaginalis strains with similar biological characteristics. furthermore, the distribution of endobionts such as mycoplasmas or trichomonas vaginalis virus (tvv) in the t. vaginalis metapopulation is poorly characterised. | 2001 | 11734059 |
| synthesis and in vitro trichomonacidal activities of some new dialkylperoxides and 1,2,4-trioxanes. | two series of three trioxanes and 18 disubstituted peroxides were synthesised and evaluated for their in vitro trichomonacidal activity against trichomonas vaginalis. the most active compound, 2-methylprop-2-yl 2-methoxyeth-1-yl peroxide exhibited an ic(50) value of 1.0+/-0.2 microm whereas other dialkyl peroxides had various ic(50) values which could not be correlated to their molecule structure. the best compound was about five times more active than metronidazole. the amount of generated oxyg ... | 2001 | 11738490 |
| survey of urinary schistosomiasis and trichomoniasis in a rural community in edo state, nigeria. | survey of urinary schistosomiasis and trichomoniasis was conducted among 830 inhabitants of ikao village, in owan local government area of edo state, nigeria between october, 1999 and february, 2000. of these, 178 (21.4%) excreted schistosoma haematobium ova in their urine. school children were more infected than the farmers and petty traders. males were more infected than the female counterparts, these differences were statistically significant using chi-square test analysis (chi 2). most of th ... | 2001 | 12170944 |
| bacterial vaginosis and other asymptomatic vaginal infections in pregnancy. | preterm birth is a common cause of neonatal morbidity and mortality. many asymptomatic genital infections have been associated with preterm birth, but attempts to determine a causal relationship between specific infections and preterm birth have been disappointing. treatment trials of specific infections have generally failed to show a positive effect, and in some trials have shown a deleterious effect. although there is a strong association between the presence of bacterial vaginosis and tricho ... | 2001 | 12112946 |
| techniques of testing for sexually transmitted diseases. | adolescent and young adult women are the highest-risk group for nearly all sexually transmitted infections. this article reviews diagnostic methods for the most common bacterial and viral sexually transmitted infections, with special attention to the use of nucleic acid amplification methods, as well as the utilization of nontraditional clinical specimens. these new modalities should help the care provider identify and manage the large asymptomatic pool of infected patients, and further lower th ... | 2001 | 12112957 |
| prevalence of trichomonas vaginalis in patients with vaginal discharge in lagos, nigeria. | trichomonas vaginalis is the most prevalent, non-viral sexually transmitted infection worldwide and probably the most commonly treatable in africa and worldwide. the purpose of this study was to determine the prevalence of t. vaginalis among women with vaginal discharge attending a gynaecologic clinic in lagos and to identify the common characteristics of the vaginal discharge in t vaginalis. high vaginal swabs were taken from consenting adults with vaginal discharge. the quantity, colour and od ... | 2001 | 11922025 |
| meeting report: anaerobic protozoan parasites, prague, czech republic, july 15-19, 2001. | 2001 | 11822654 | |
| mechanism and clinical significance of metronidazole resistance in helicobacter pylori. | metronidazole was introduced in 1959 for the treatment of trichomonas vaginalis, but was subsequently shown to be active against anaerobic and some micro-aerophilic bacteria as well. in anaerobic microorganisms with their low redox potential, metronidazole is reduced to its active metabolite by a one-electron transfer step. metronidazole is often used in treatment regimens for helicobacter pylori, a microaerophilic bacterium, but resistance to this drug is frequently encountered. the metabolism ... | 2001 | 11768554 |
| tubulins in trichomonas vaginalis: molecular characterization of alpha-tubulin genes, posttranslational modifications, and homology modeling of the tubulin dimer. | we have isolated and analysed an alpha-tubulin-encoding gene (atub1) in an early-diverging eukaryote, trichomonas vaginalis. the complete atub1 open reading frame included 1.356 bp encoding a polypeptide of 452 amino-acyl residues. a second alpha-tubulin gene (atub2) was amplified by pcr using primers derived from consensus alpha-tubulin amino acid sequences. both t. vaginalis alpha-tubulin sequences showed high identity to those described in other parabasalids (94.4%-97.3%), and exhibited a hig ... | 2001 | 11831773 |
| trichomonas vaginalis, hiv, and african-americans. | trichomonas vaginalis may be emerging as one of the most important cofactors in amplifying hiv transmission, particularly in african-american communities of the united states. in a person co-infected with hiv, the pathology induced by t. vaginalis infection can increase hiv shedding. trichomonas infection may also act to expand the portal of entry for hiv in an hiv-negative person. studies from africa have suggested that t. vaginalis infection may increase the rate of hiv transmission by approxi ... | 2001 | 11747718 |
| unique phylogenetic relationships of glucokinase and glucosephosphate isomerase of the amitochondriate eukaryotes giardia intestinalis, spironucleus barkhanus and trichomonas vaginalis. | glucokinase (gk) and glucosephosphate isomerase (gpi), the first two enzymes of the glycolytic pathway of the diplomonads giardia intestinalis and spironucleus barkhanus, type i amitochondriate eukaryotes, were sequenced. gpi of the parabasalid trichomonas vaginalis was also sequenced. the diplomonad gks belong to a family of specific gks present in cyanobacteria, in some proteobacteria and also in t. vaginalis, a type ii amitochondriate protist. these enzymes are not part of the hexokinase fami ... | 2001 | 11750134 |
| a clinical study on the association of trichomonas vaginalis and mycoplasma hominis infections in women attending a sexually transmitted disease (std) outpatient clinic. | swabs from the posterior vaginal fornix were obtained from 804 consecutive female patients visiting a large dutch sexually transmitted diseases (std) outpatient clinic. a detailed clinical history was obtained and complaints concerning the lower genital tract, such as vaginal discharge or vulval and vaginal irritation, were recorded. patients were examined and the presence of non-physiological vaginal secretions was established by speculum examination. the swabs were monitored for bacterial vagi ... | 2001 | 11750218 |
| in-pouch tv culture system in diagnosis of trichomonas vaginalis infection. | a newly simplified culture method, the in-pouch tv culture system, the wet mount (wm) examination and the acridine orange stain were compared with the oxoid culture as a standard technique in the diagnosis of trichomoniasis. out of 70 symptomatic cases enrolled in this study, 28 specimens were positive by all methods. among these positive specimens, 21 were positive by the oxoid culture (75%) and 24 (85.7%) with sensitivity of 85.7% by the in-pouch system. both wet mount preparation and acridine ... | 2001 | 11775092 |
| evaluation of the effect of a plant alkaloid (berberine derived from berberis aristata) on trichomonas vaginalis in vitro. | berberine is a quaternary alkaloid derived from the plant berberis aristata having antibacterial, antiamoebic, antifungal, antihelminthic, leishmanicidal and tuberculostatic properties. the effect of berberine sulphate salt on the growth of trichomonas vaginalis in vitro was compared to the efficacy of metronidazole as a reference drug. results showed that berberine sulphate was comparable to metronidazole as regards potency with the advantage of being more safe and possible replacement in metro ... | 2001 | 11775115 |
| trichomonas vaginalis in vaginal smears of women using intrauterine contraceptive device. | to detect the association between the use of an intrauterine contraceptive device (iucd) and the presence of trichomonas vaginalis (tv), vaginal smears of 1110 women were examined cytologically. among 45 iucd users, 10 women were positive for tv (22.22%). forty six of 1065 non-users were also positive for tv (4.32%). there was a significant correlation between the use of iucd and the presence of tv (p < 0.05). the data also indicated that the prolonged use (> or = 3 years) of copper-iucd may pro ... | 2001 | 11787243 |
| antimicrobial activity of castanopsis acuminatissima. | the methanol extracts of castanopsis acuminatissima leaves, stem and root barks were partitioned (petrol, dichloromethane, ethyl acetate). though all of the crude methanolic extracts and obtained fractions from them, showed a broad spectrum of antibacterial activity, in most cases the activity was decreased on fractionation. none was active against tested moulds. | 2001 | 11223229 |
| screening for sexually transmitted diseases in human immunodeficiency virus-positive patients in peru reveals an absence of chlamydia trachomatis and identifies trichomonas vaginalis in pharyngeal specimens. | to determine the prevalence of sexually transmitted diseases (stds), we screened 107 human immunodeficiency virus-positive patients in peru, where the virus is predominantly sexually transmitted. patients had multiple risk factors for stds, and 38% of women and 50% of men had at least 1 std (gonorrhea, trichomoniasis, herpes simplex, anogenital warts, or syphilis seropositivity). no chlamydial infection was detected, even though infection rates in the general population are 5%-12%. patients rece ... | 2001 | 11229850 |
| differences between coding and non-coding regions in the trichomonas vaginalis genome: an actin gene as a locus model(1). | the sequence of a cloned genomic fragment of trichomonas vaginalis containing a complete actin gene was determined. an uninterrupted open reading frame of 1128 nucleotides was found that codes for an actin gene. two overlapped consensus promoter sequences for t. vaginalis were found 12 nucleotides upstream the actin initiation codon. in addition to actin, two incomplete open reading frames were found at the 5' and 3' ends of the clone. these two sequences are expressed and showed similarity to a ... | 2001 | 11230824 |
| a rural hiv epidemic in zimbabwe? findings from a population-based survey. | the aim of the study was to use population-based data from 689 adults to describe the socio-demographic, behavioural and biomedical correlates of hiv infection and aid identification of effective hiv control strategies for rural zimbabwe. dried blood spot and urine samples were collected for hiv and sexually transmitted disease (std) testing and participants were interviewed on socio-demographic characteristics, sexual behaviour and experience of std symptoms. hiv seroprevalence was 23.3% and wa ... | 2001 | 11231873 |
| prevalence of 5-nitroimidazole-resistant trichomonas vaginalis in oviedo, spain. | 2001 | 11234785 | |
| drug targets and mechanisms of resistance in the anaerobic protozoa. | the anaerobic protozoa giardia duodenalis, trichomonas vaginalis, and entamoeba histolytica infect up to a billion people each year. g. duodenalis and e. histolytica are primarily pathogens of the intestinal tract, although e. histolytica can form abscesses and invade other organs, where it can be fatal if left untreated. t. vaginalis infection is a sexually transmitted infection causing vaginitis and acute inflammatory disease of the genital mucosa. t. vaginalis has also been reported in the ur ... | 2001 | 11148007 |
| urine proves a poor specimen for culture of trichomonas vaginalis in women. | 2001 | 11158705 | |
| reproductive tract infections among married women in upper egypt. | in a house-to-house survey using cluster sampling, 1344 married women from urban and rural areas of upper egypt (minia, assiut and sohag) were interviewed and examined to study the magnitude and determinants of reproductive tract infections. overall prevalence was found to be 52.8%, with the most prevalent forms being candida albicans (28.0%), trichomonas vaginalis (8.7%), aspergillus species (7.4%), streptococci (4.6%) and chlamydia trachomatis (4.2%). multivariate analysis identified certain g ... | 2001 | 12596963 |
| [detection of dna of toxoplasma gondii in rat by using polymerase chain reaction]. | to study the significance of dna of toxoplasma gondii in peripheral blood. | 2001 | 12571947 |
| [antigen analysis of trichomonas vaginalis trophozoite by sds-page and two-dimensional gel electrophoresis]. | to analyze soluble antigens of trichomonas vaginalis. | 2001 | 12571968 |
| [a modified method for preparation of trichomonas vaginalis specimen]. | 2001 | 12572028 | |
| [studies on ultrastructure and histochemistry of trichomonas vaginalis adhering to vaginal mucosa of rats]. | to study interaction between trichomonas vaginalis and epithelium of genital tract of host as well as the pathogenesis of t. vaginalis. | 2001 | 12572044 |
| knowledge, attitude and practice about sexually transmitted diseases among university students in kampala. | sexually transmitted diseases (stds) remain an important cause of morbidity and mortality among women in the child-bearing age. in order to institute appropriate preventive measures there is need to establish the profile of knowledge of the predisposing factors and causation of stds, attitude to sexual practice and sexual patterns among the susceptible young people, such as university students. | 2001 | 12789128 |
| [systematic search for parasites among leprosy patients in mali]. | practice of multidrug therapy in leprosy (combination dapsone + rifampicine + clofazimine) established since 1981, has significantly reduced the incidence of the disease. however, immunosuppression due to treatment of multi-drugs therapy induced adverse reactions with glucocorticoid and the change in host immune response due to the leprosy itself, might increase the risk of parasitic infections. to test this hypothesis, we carried out a case-control study at the "institut marchoux" in bamako. st ... | 2002 | 12616942 |
| prevalence of asymptomatic genital infection among pregnant women in benin city, nigeria. | the prevalence of asymptomatic genital infection among pregnant women and their susceptibility to antibacterial agents was investigated to provide baseline data on common asymptomatic genital microorganisms and identify potentials for development of clinical disease among this cohort of patients. high vaginal swabs were obtained from five hundred consecutive and consenting pregnant women attending the antenatal clinic of the university of benin teaching hospital (ubth) and the central hospital, ... | 2002 | 12685413 |
| optimization of random amplified polymorphic dna techniques for its use in genetic studies of trichomonas vaginalis isolates. | the random amplified polymorphic dna (rapd) technique is a simple method to detect dna polymorphism. several factors can affect the amplification profiles causing the presence of false bands and assay non-reproducibility. in this study, we analyzed the effect of changing concentrations of the primer, template dna and taq dna polymerase with the goal of determining their optimum concentration for the standardization of the rapd technique for genetic studies of trichomonas vaginalis, a parasite th ... | 2002 | 12798003 |
| [evaluation of direct microscopic examination, acridine orange staining and culture methods for studies of trichomonas vaginalis in vaginal discharge specimens]. | trichomonas vaginalis is the causative agent of human trichomoniasis which is a sexually transmitted disease mainly in women. the infection may be asymptomatic or symptomatic such as severe vaginitis and cervicitis. the aim of this study was to compare direct microscopic examination, acridine orange stained examination and culture in modified diamond medium, for the detection of t. vaginalis from the vaginal swab samples of 310 patients (age ranges: 17-45 years old) who were complaining from vag ... | 2002 | 12838668 |
| role of the vaginal microbiological ecosystem and cytokine profile in the promotion of cervical dysplasia: a case-control study. | to identify alterations in the cytokine profile and microbial ecosystem of the vagina in association with cervical dysplasia. | 2002 | 12648311 |
| synthesis, antiprotozoal and anticancer activity of substituted 2-trifluoromethyl- and 2-pentafluoroethylbenzimidazoles. | the synthesis of several halogenated benzimidazoles substituted in position 2 with trifluoromethyl, pentafluoroethyl and 2-thioethylaminodimethyl group is reported. antiprotozoal and anticancer activity of series of newly synthesized and previously obtained compounds was studied. all of tested bezimidazoles showed remarkable antiprotozoal activity against giardia intestinalis, entamoeba histolytica and trichomonas vaginalis. of the studied collection of halogenated benzimidazoles the most antica ... | 2002 | 12660022 |
| trichomonas vaginalis: microtubule cytoskeleton distribution using fluorescent taxoid. | trichomonas vaginalis is a flagellated parasitic protist of the human urogenital tract. the parasite has a poorly known cytoskeleton formed by an axostyle and a pelta, which are formed by stable structures such as microtubules, essential for the maintenance of cell shape and organization. flutax-2 is an active fluorescent derivative of taxol, binds to alphabeta-tubulin dimer polymerized. in this paper we present the analysis of microtubule distribution in living trophozoites of t. vaginalis usin ... | 2002 | 12706747 |
| [association between mycoplasma genitalium and preterm labor]. | m. genitalium was identified by using polymerase chain reaction (pcr). the samples were obtained from the posterior vaginal fornix between 21-25 gestation weeks and investigated for ph, gram stain for bacterial vaginosis; chlamidia trachomartis; neisseria gonorrhoeae; trichomonas vaginalis. of 102 pregnant women, only five had a pcr-positive for m. genitalium. the occurrence of m. genitalium in posterior vaginal fornix at midtrimester is infrequent in pregnant women and unlikely to be a contribu ... | 2002 | 12577501 |
| [comparative effect of different media in in vitro cultivation of trichomonas vaginalis]. | to investigate the optimal condition for in vitro cultivation of trichomonas vaginalis for obtaining a better harvest of t. vaginalis. | 2002 | 12567545 |
| self-collected vaginal swabs for the detection of multiple sexually transmitted infections in adolescent girls. | to evaluate the use of self-collected vaginal swabs to test for neisseria gonorrhoeae, chlamydia trachomatis, and trichomonas vaginalis, and to describe the acceptability of this technique to adolescents. | 2002 | 12547662 |
| the chitinase system from trichomonas vaginalis as a potential target for antimicrobial therapy of urogenital trichomoniasis. | chitinolytic activities in trichomonas vaginalis membrane extracts were assessed by assays of three enzyme systems: n-acetyl-beta-d-hexosaminidase (nahase), chitobiosidase and chitotriosidase. n-acetyl-beta-d-hexosaminidase was the enzyme that showed the highest specific activity. after successive subcutaneous inoculations into mice and parasite recovery in culture, the enzyme activities increased significantly with the number of inoculations for up to eight passages. in addition, enzyme activit ... | 2002 | 12504272 |
| effect of the iron chelator deferoxamine on trichomonas vaginalis in vitro. | the effect of 10 micromol, 15 micromol, 30 micromol, and 60 micromol concentrations of deferoxamine (dfo), a clinically approved iron chelator, was determined on viability and multiplication of trichomonas vaginalis grown in tym axenic culture medium at 24 hours interval. dfo killed all t. vaginalis isolates with a minimum lethal concentration of 30 micromol after 48 hours culture incubation with the drug. a potent and persistent inhibitory effect of dfo on the parasite viability and multiplicat ... | 2002 | 12512803 |
| 1,3-diphenylpyrazoles: synthesis and antiparasitic activities of azomethine derivatives. | 1,3-diphenylpyrazole-4-carboxaldehyde and 1-(4-nitrophenyl)-3-phenylpyrazole-4-carboxaldehyde were obtained from the appropriated phenylhydrazones via the vilsmeier-haack reaction. these two aldehydes were functionalized by various substituted anilines or substituted benzylamines. antiparasitic activities of the corresponding azomethines were assessed. in the most cases, nitrated compounds were found to be more efficient than non-nitrated ones against plasmodium falciparum, trichomonas vaginalis ... | 2002 | 12161064 |
| trichomonas vaginalis infection in a premature newborn. | trichomonas vaginalis is a vaginal pathogen associated with an increased incidence of adverse outcomes of pregnancy. data are limited regarding the effects of trichomonas infections in the newborn. we report a symptomatic t. vaginalis infection in a 26-week premature infant and a review of the literature. | 2002 | 12168131 |
| improved detection by dna amplification of trichomonas vaginalis in males. | trichomoniasis is a sexually transmitted infection that is highly prevalent worldwide and has been linked to preterm birth and human immunodeficiency virus acquisition. in females, trichomoniasis causes vaginitis, while in males, it is frequently asymptomatic but can be a cause of urethritis. control efforts have been hampered by the lack of a sensitive diagnostic technique for this infection in males. men attending a sexually transmitted disease (std) clinic for a new complaint were screened fo ... | 2002 | 12354865 |
| epidemiological features of women with trichomoniasis in auckland sexual health clinics: 1998-99. | to determine some epidemiological features of female sexually transmitted disease (std) clinic attendees with trichomonas vaginalis infection. | 2002 | 12362164 |
| resistance of trichomonas vaginalis infections to metronidazole in auckland sexual health clinics: report of two cases. | 2002 | 12362204 | |
| polyamine metabolism as chemotherapeutic target in protozoan parasites. | polyamines are essential cell constituents for all organisms. the present review highlights important differences in the synthesis, degradation, and interconversion of polyamines between the protozoan parasites (trypanosoma brucei, trypanosoma cruzi, cryptosporidium parvum and trichomonas vaginalis) and their mammalian hosts. approaches include development of mono- and di-substituted polyamine analogs targeting polyamine interconversion, as well as more traditional targeting of synthetic enzymes ... | 2002 | 12370040 |
| characterization of an ecto-phosphatase activity in the human parasite trichomonas vaginalis. | we have characterized phosphatase activity present on the external surface of trichomonas vaginalis, using intact living parasites. this enzyme hydrolyzes the substrate p-nitrophenylphosphate (p-npp) at a rate of 134.3+/-14.8 nmol pi/h per 10(7) cells. this phosphatase activity decreased by increasing the ph from 6.8 to 8.4, a ph range in which cell viability was maintained for at least 1 h. experiments using classical inhibitors of acid phosphatases, such as ammonium molybdate and sodium fluori ... | 2002 | 12375165 |
| the epidemiology of vaginal colonisation with group b streptococci in a sexually transmitted disease clinic. | to determine whether group b streptococcus (gbs) infection is sexually transmitted and whether colonisation with gbs could be related to vaginal symptoms or signs. | 2002 | 12381483 |
| biological variability in clinical isolates of trichomonas vaginalis. | eighteen clinical isolates of trichomonas vaginalis were obtained from women who attended health centers of the government of madrid. a total of 1,848 vaginal specimens recovered during the gynaecological examination were seeded in culture tubes containing liquid diamond medium. pathogenicity to mice was determined after intraperitoneal inoculation of mice by quantification of mortality and gross damage to abdominal organs. as could be expected, a broad variability was obtained, being some of th ... | 2002 | 12386717 |
| demonstration of trichomonas vaginalis in tracheal aspirates in infants with early respiratory failure. | trichomonas vaginalis was isolated from the tracheal aspirates of two premature newborns with early respiratory failure who were delivered vaginally by mothers with t. vaginalis infection. the babies were treated successfully with antiparasitic drugs. | 2002 | 12389679 |
| trichomonas vaginalis: observation of coexistence of multiple viruses in the same isolate. | trichomonas vaginalis is a flagellated, parasitic protozoan that inhabits the urogenital tract of humans. some isolates of t. vaginalis are infected with a double-stranded rna (dsrna) virus, which was described in the literature as homogeneous icosahedral viral particles with an isometric symmetry and 33 nm in diameter. this study examined in detail the viral particles in t. vaginalis isolate 347 and describes a heterogeneous population of viral particles. the different dsrna viruses were only o ... | 2002 | 12399035 |
| antimicrobial activity of dequalinium chloride against leading germs of vaginal infections. | dequalinium chloride (cas 522-51-0) and povidone iodine (cas 25655-41-8) are known as antiseptic agents and used in the local treatment of vaginal infections. clotrimazole (cas 23593-75-1) is an anti-fungal drug and applied primarily in the therapy of vulvo-vaginal candidiasis and to a lesser extent in bacterial vaginosis and trichomoniasis. however, antimicrobial activities of those three agents in comparison to each other have not been reported so far. to address this issue the antimicrobial a ... | 2002 | 12404886 |
| predictors of infection with trichomonas vaginalis: a prospective study of low income african-american adolescent females. | to identify psychosocial predictors of trichomonas vaginalis infection among low income african-american adolescent females living in a high risk urban area of the united states. | 2002 | 12407241 |
| post-treatment sexual and prevention behaviours of adolescents with sexually transmitted infections. | to evaluate sexual behaviour (including abstinence), sex partner change, and condom use during the 3 month period following treatment for neisseria gonorrhoeae, chlamydia trachomatis, trichomonas vaginalis, or non-gonococcal urethritis. | 2002 | 12407242 |
| does contraceptive method change the pap smear finding? | the aim of the present study was to investigate the effect of various types of contraceptives on morphological finding of pap smear results. pap smear results of 7,753 fertile women who presented to 15 different family planning services in fars province (south of iran) were analyzed according to their methods of contraception. the population consisted of 2241 women who used intrauterine device (iud) as a contraceptive method (group 1), 2521 women who were oral contraceptive (ocp) users (group 2) ... | 2002 | 12413619 |
| gang involvement and the health of african american female adolescents. | to examine the association between gang involvement and female adolescents' health. | 2002 | 12415063 |
| synthesis and in vitro antiprotozoal activity of 5-nitrothiophene-2-carboxaldehyde thiosemicarbazone derivatives. | several thiosemicarbazone derivatives of 5-nitrothiophene-2-carboxaldehyde were prepared by the simple process in which n(4)-thiosemicarbazone moiety was replaced by aliphatic, arylic and cyclic amine. among these thiosemicarbazones compound 11 showed significant antiamoebic activity whereas compound 3 was more active antitrichomonal than the reference drug. | 2002 | 12419387 |
| 'in vitro' study of chemotherapeutic activity of sulphimidazole on some sensitive and metronidazole-resistant trichomonas vaginalis strains. | trichomonacidal treatment based on 5-nitroimidazoles is problematic both when metronidazole, the drug of choice, is ineffective owing to the presence of resistant strains and when bacterial aerobic infections are present. sulphimidazole (siz) possesses two distinct functional groups: one sulphonamide, the other 5-nitroimidazole. since siz is active against aerobic and anaerobic bacteria, we set out to discover whether, in view of the presence of the 5-nitroimidazole group, it could also be effec ... | 2002 | 12419652 |