Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| kinetic analysis of dehydropeptidase-i and comparative in vitro and in vivo stabilities of ps-5 derivatives modified at the c-3 side chain. | with partially purified kidney dehydropeptidase-i (dhp-i) preparations, the hydrolysis kinetics of glycyldehydrophenylalanine (gly-dph) by dhp-i were found to be completely non-michaelian, whereas those of ps-5 and imipenem were composed of at least two-phase reactions which were also observed using bacillus cereus type ii beta-lactamase. thus the dhp-i stabilities of 34 ps-5 carbapenem derivatives which were synthesized by chemical modification at the c-3 side chain of ps-5 were examined in vit ... | 1991 | 2054858 |
| transduction of certain genes by an autonomously replicating bacillus thuringiensis phage. | a derivative of bacillus thuringiensis subsp. kurstaki hd1 (hd1-9) released transducing phage (tp21) from late exponential cultures. three of seven markers tested were transduced into bacillus cereus, but only two of these (cysc and trpb/f) were transduced at a frequency of more than 100 times the reversion rates. a limited transduction capacity was given further support in that few chromosomal markers were carried in the hd1-9 lysate, as demonstrated by southern hybridization. restriction fragm ... | 1991 | 2059027 |
| toxin production by bacillus pumilus. | two strains of bacillus pumilus (m11 and m38) and one strain each of bacillus cereus (m27), bacillus subtilis (m67), and enterobacter agglomerans (m14) were identified from the air of lancashire cotton mills. these strains were tested for cytopathic effects in vero cells; b pumilus and b cereus strains were also examined for haemolytic activity, lecithinase production, and proteolytic action on casein. rounding and clumping of the vero cells occurred after the addition of supernatants prepared f ... | 1991 | 2066422 |
| purification and characterization of neuron-specific surface antigen defined by monoclonal antibody bm88. | monoclonal antibody bm88 recognizes a neurospecific surface antigen in the cns and the pns. in the present study, the antigen recognized by bm88 was immunopurified from pig brain and shown to be a 22-kda polypeptide by reducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis. under nonreducing conditions a protein of 40 kda was obtained, a result indicating that the antigen is composed of two polypeptide chains of equal molecular weight linked by disulfide bridges. gel filtration of th ... | 1991 | 1704420 |
| crystal structure of penicillium citrinum p1 nuclease at 2.8 a resolution. | p1 nuclease from penicillium citrinum is a zinc dependent glyco-enzyme consisting of 270 amino acid residues which cleaves single-stranded rna and dna into 5'-mononucleotides. the x-ray structure of a tetragonal crystal form of the enzyme with two molecules per asymmetric unit has been solved at 3.3 and refined at 2.8 a resolution to a crystallographic r-factor of 21.6%. the current model consists of 269 amino acid residues, three zn ions and two n-acetyl glucosamines per subunit. the enzyme is ... | 1991 | 1710977 |
| comparative analysis of bacillus anthracis, bacillus cereus, and related species on the basis of reverse transcriptase sequencing of 16s rrna. | the primary structures of the 16s rrnas of bacillus anthracis, bacillus cereus, bacillus mycoides, and bacillus thuringiensis were determined by using the reverse transcription-dideoxy sequencing method. all of the strains exhibited very high levels of sequence similarity (greater than 99%) that were consistent with the close relationships shown by previous dna hybridization studies. the sequences of b. anthracis sterne and b. cereus emetic strain nctc 11143 were found to be identical for a cont ... | 1991 | 1715736 |
| lipid globule staining to aid in differentiating bacillus species. | the use of the lipid globule stain to aid in differentiating the bacillus cereus group (i.e., b. cereus, b. cereus var. mycoides, and b. thuringiensis) from other bacillus species was investigated. smears from colonies grown on suitable agar were made on precleaned slides, stained, and examined microscopically for characteristic deep blue lipid globules. the study included a total of 649 cultures of bacillus species plus 143 incompletely characterized bacillus isolates from food. only b. cereus, ... | 1991 | 1717433 |
| blasticidin s-resistance gene (bsr): a novel selectable marker for mammalian cells. | blasticidin s is a microbial antibiotic that inhibits protein synthesis in both prokaryotes and eukaryotes. the blasticidin s-resistance gene (bsr), isolated from bacillus cereus k55-s1 strain, was inserted into psv2 plasmid vector and introduced into cultured mammalian cells by transfection. the bsr gene was integrated into the genome and conferred blasticidin s resistance on hela cells. the transfection frequency of the bsr gene was as high as that of the aminoglycoside phosphotransferase gene ... | 1991 | 1720391 |
| identification of phosphatidylinositol-specific phospholipase c activity in listeria monocytogenes: a novel type of virulence factor? | a phospholipase c which cleaves phosphatidylinositol and glycosylphosphatidylinositol (gpi) anchors was identified in listeria monocytogenes. this 36 kda protein is encoded by the gene plca, and is homologous to the bacillus cereus, bacillus thuringiensis and eukaryotic phosphatidylinositol-specific phospholipases c (pi-plc). expression of the plca gene in escherichia coli correlates with the appearance of pi-plc activity in the cells. in listeria monocytogenes, the activity is secreted to the c ... | 1991 | 1645839 |
| phosphatidylinositol-specific phospholipases c from bacillus cereus and bacillus thuringiensis. | 1991 | 1646937 | |
| beta-lactamase inhibiting properties of 6-oxopenicillanic and 7-oxo-cephalosporanic acid derivatives. | 1991 | 1649611 | |
| the possible involvement of trypsin-like enzymes in germination of spores of bacillus cereus t and bacillus subtilis 168. | germination of spores of bacillus cereus t and bacillus subtilis 168 was inhibited by the trypsin inhibitors leupeptin and tosyllysine chloromethyl ketone (tlck) and by the substrates tosylarginine methyl ester (tame), benzoyl-l-arginine-p-nitroanilide (l-bapna) and d-bapna. potencies of these inhibitory compounds were estimated by finding the concentration which inhibited 50% germination (id50), as measured by events occurring early (loss of heat resistance), at an intermediate stage [dipicolin ... | 1991 | 1650815 |
| two different aa3-type cytochromes can be purified from the bacterium bacillus cereus. | two aa3-type cytochromes were purified from membranes of sporulating bacillus cereus. one of them, an aa3 complex, was found to be composed of two subunits (51 and 31 kda), two a hemes and three copper atoms, thus being similar to the cytochrome aa3 previously purified from vegetative b. cereus [garcía-horsman, j. a., barquera, b., gonzález-halphen, d. & escamilla, j. e. (1991) mol. microbiol. 5, 197-205]. the second isoform, a caa3 complex, was expressed in sporulating cells only, and was found ... | 1991 | 1651246 |
| role of gtpase activating protein in mitogenic signalling through phosphatidylcholine-hydrolysing phospholipase c. | recent evidence has accumulated showing that activation of plc-catalysed hydrolysis of phosphatidylcholine (pc-plc) is a critical step in mitogenic signal transduction both in fibroblasts and in oocytes from xenopus laevis. the products of ras genes activate pc-plc, bind guanine nucleotides, have intrinsic gtpase activity, and are regulated by a gtpase-activating protein (gap). it has been suggested that, in addition to its regulatory properties, gap may also be necessary for ras function as a d ... | 1991 | 1655413 |
| a surface antigen of giardia lamblia with a glycosylphosphatidylinositol anchor. | since giardia lamblia trophozoites are exposed to high concentrations of fatty acids in their human small intestinal milieu, we determined the pattern of incorporation of [3h]palmitic acid and myristic acid into g. lamblia proteins. the pattern of fatty acylation was unusually simple since greater than 90% of the giardia protein biosynthetically labeled with either [3h]palmitate or myristate migrated at approximately 49 kda (gp49) in reducing sodium dodecyl sulfate-polyacrylamide gel electrophor ... | 1991 | 1657957 |
| glycosyl-phosphatidylinositol anchored acetylcholinesterase as substrate for phosphatidylinositol-specific phospholipase c from bacillus cereus. | we investigated the enzymatic properties of phosphatidylinositol-specific phospholipase c (pi-plc) from bacillus cereus towards glycosyl-phosphatidylinositol anchored acetylcholinesterase (ache) from bovine erythrocytes and torpedo electric organ as substrate. the conversion of membrane from ache to soluble ache by pi-plc depended on the presence of a detergent and of phosphatidylcholine. in presence of mixed micelles containing triton x-100 (0.05%) and phosphatidylcholine (0.5 mg/ml) the rate o ... | 1991 | 1660725 |
| high-level expression in escherichia coli and rapid purification of phosphatidylinositol-specific phospholipase c from bacillus cereus and bacillus thuringiensis. | the construction of four vectors for high-level expression in escherichia coli of the phosphatidylinositol-specific phospholipase c from bacillus cereus or bacillus thuringiensis is described. in all constructs the coding sequence for the mature phospholipase is precisely fused to the e. coli heat-stable enterotoxin ii signal sequence for targeting of the protein to the periplasm. in one set of plasmids expression of the b. cereus or b. thuringiensis enzyme is under control of the e. coli alkali ... | 1991 | 1668269 |
| fried rice from the take-away. | 1991 | 1669788 | |
| purification and characterization of a repressor for the bacillus cereus glnra operon. | we report the overexpression, purification, and properties of the regulatory protein, glnr, for glutamine synthetase synthesis of bacillus cereus. the protein was found to be a dimer with a molecular weight of approximately 30,000, and its subunit molecular weight was 15,000 in agreement with that (15,025) of deduced amino acid sequence of glnr. the purified glnr protein bound specifically to the promoter region of the glnra operon of b. cereus and bacillus subtilis. the binding of the glnr prot ... | 1991 | 1677938 |
| detection of bacillus cereus flagellar antigen by enzyme-linked immunosorbent assay (elisa) | a serological typing scheme of bacillus cereus has been developed by immunochemical analyses of flagellar antigen using an agglutination method. enzyme-linked immunosorbent assay (elisa) for the classification of flagellar serotype of bacillus cereus had greater sensitivity. 10-500 times, than that of agglutination method. the specificity of flagellar antigen and antibody was determined by immunogold electron microscopy and elisa inhibition assay. application of elisa is useful for the detection ... | 1991 | 1678490 |
| deoxyribose 5-phosphate aldolase of bacillus cereus: purification and properties. | deoxyribose 5-phosphate aldolase was purified 41 times from bacillus cereus induced by growth on deoxyribonucleosides. the purification procedure includes ammonium sulphate fractionation, gel filtration on sephadex g-100, ion-exchange chromatography on deae-sephacel and preparative electrophoresis on 10% polyacrylamide gel. the enzyme is stable above ph 6.5, but is rapidly inactivated by sulfhydryl reagents. being insensitive to edta, it may be considered as a class i aldolase. among a number of ... | 1992 | 1730028 |
| critical micelle concentration and hemolytic activity--a correlation suggested by the marine sterol, halistanol trisulfate. | the marine natural product, halistanol trisulfate, has a relatively low critical micelle concentration of 0.001% m/v (14.5 microm) and strong hemolytic potency with an ec50 of 0.00046% m/v (6.67 microm). as expected of a detergent, it inhibits the growth of gram-positive but not gram-negative bacteria. the hemolytic activity of halistanol trisulfate and other detergents has been shown to correlate with critical micelle concentration. this correlation may have important implications in the mechan ... | 1992 | 1731774 |
| studies on kinetic model of vitamin c two-step fermentation process. | this paper aims at analyzing the bioconversion process from l-sorbose to 2-keto-l-gulonic acid with the aid of the growth factor assumption. fermentation mechanism is discussed after making necessary simplifications. a model is established for this second step fermentation process. nonlinear optimization together with runge-kutta method, are used to obtain model parameters. results of computer simulation agree with experimental data to show the reliability of the kinetic model. | 1992 | 1295600 |
| nucleotide sequence of the lecithinase operon of listeria monocytogenes and possible role of lecithinase in cell-to-cell spread. | the lecithinase gene of the intracellular pathogen listeria monocytogenes, plcb, was identified in a 5,648-bp dna fragment which expressed lecithinase activity when cloned into escherichia coli. this fragment is located immediately downstream of the previously identified gene mpl (prta). it contains five open reading frames, named acta, plcb, and orfx, -y, and -z, which, together with mpl, form an operon, since a 5.7-kb-long transcript originates from a promoter located upstream of mpl (j. menga ... | 1992 | 1309513 |
| phospholipase c-mediated hydrolysis of phosphatidylcholine is a target of transforming growth factor beta 1 inhibitory signals. | cell growth and tumor transformation can be restrained in certain cell systems by the action of transforming growth factor beta (tgf-beta). it has been established that the mechanism whereby tgf-beta 1 inhibits cell growth does not interfere with the triggering of early mitogenic signal transduction mechanisms. phospholipase c-catalyzed hydrolysis of phosphatidylcholine (pc) is a relatively late step in the cascade activated by growth factors. therefore, conceivably activation of phospholipase c ... | 1992 | 1309592 |
| bce83i, a restriction endonuclease from bacillus cereus 83 which recognizes novel nonpalindromic sequence 5'-cttgag-3' and is stimulated by s-adenosylmethionine. | 1992 | 1315959 | |
| phospholipids chiral at phosphorus. stereochemical mechanism for the formation of inositol 1-phosphate catalyzed by phosphatidylinositol-specific phospholipase c. | the phosphatidylinositol-specific phospholipase c (pi-plc) from mammalian sources catalyzes the simultaneous formation of both inositol 1,2-cyclic phosphate (icp) and inositol 1-phosphate (ip). it has not been established whether the two products are formed in sequential or parallel reactions, even though the latter has been favored in previous reports. this problem was investigated by using a stereochemical approach. diastereomers of 1,2-dipalmitoyl-sn-glycero-3-(1d- [16o,17o]phosphoinositol) ( ... | 1992 | 1318746 |
| characterization of a zn(2+)-requiring glycerophosphocholine cholinephosphodiesterase possessing p-nitrophenylphosphocholine phosphodiesterase activity. | p-nitrophenylphosphocholine phosphodiesterase activity was purified 5000-fold from mouse brain by treatment of membranes with bacillus cereus phospholipase c preparation and sequential chromatographies on concanavalin a-sepharose and cm-sephadex columns. the phosphodiesterase (zn(2+)-requiring) showed km and vmax. values of 5.5 microm and 4.2 mumol/min per mg respectively in the hydrolysis of p-nitrophenylphosphocholine, and possessed an optimum ph of 10.5 and a molecular mass of approx. 74 kda. ... | 1992 | 1326942 |
| substrate stereospecificity of phosphatidylinositol-specific phospholipase c from bacillus cereus examined using the resolved enantiomers of synthetic myo-inositol 1-(4-nitrophenyl phosphate). | the substrate stereospecificity of phosphatidylinositol-specific phospholipase c from bacillus cereus is examined using the resolved optical isomers of synthetic myo-inositol 1-(4-nitrophenyl phosphate), a chromogenic substrate for the phospholipase. the synthetic route employs mild acid-labile protecting groups and separation of the substituted myo-inositol enantiomers as the (-)-camphanyl ester diastereomers. measurements of the initial rates of cleavage of the d and l enantiomers of the nitro ... | 1992 | 1327107 |
| mammalian cells that express bacillus cereus phosphatidylinositol-specific phospholipase c have increased levels of inositol cyclic 1:2-phosphate, inositol 1-phosphate, and inositol 2-phosphate. | phosphatidylinositol-specific phospholipase c (ptdins-plc) of bacillus cereus catalyzes the conversion of ptdins to inositol cyclic 1:2-phosphate and diacylglycerol. nih 3t3, swiss mouse 3t3, cv-1, and cos-7 cells were transfected with a cdna encoding this enzyme, and the metabolic and cellular consequences were investigated. overexpression of ptdins-plc enzyme activity was associated with elevated levels of inositol cyclic 1:2-phosphate (2.5-70-fold), inositol 1-phosphate (2-20-fold), and inosi ... | 1992 | 1328186 |
| kinetics of bacillus cereus phosphatidylinositol-specific phospholipase c with thiophosphate and fluorescent analogs of phosphatidylinositol. | thiophosphate analogs (c-s-p bond) of phosphatidylinositol (cn-thio-pi: racemic hexadecyl-, dodecyl-, and octylthiophosphoryl-1-myo-inositol) and a fluorescent analog (pyrene-pi: rac-4-(1-pyreno)-butylphosphoryl-1-myo-inositol) were all substrates for phosphatidylinositol-specific phospholipase c from bacillus cereus. hydrolysis of thio-pi was followed by coupling the production of alkylthiol to a disulfide interchange reaction with dithiobispyridine. hydrolysis of pyrene-pi was followed using a ... | 1992 | 1333794 |
| a high level of cell surface phosphatidylinositol-specific phospholipase c activity is characteristic of growth-arrested 3t3 fibroblasts but not of transformed variants. | confluent monolayers of four contact-inhibited mouse fibroblast lines (swiss 3t3, balb/c 3t3, nih 3t3, and c3h10t1/2) were found to have substantial levels of a cell surface phosphatidylinositol-specific phospholipase c (ecto-plc). in contrast, confluent cultures of virally, chemically, or spontaneously transformed variants derived from these cell lines expressed undetectable or negligible levels of this enzyme activity. a simple and rapid assay, using lysophosphatidylinositol radio-labeled in t ... | 1992 | 1338336 |
| [cloning and gene expression of bacillus cereus neutral proteinase in bacillus subtilis cells]. | the neutral proteinase gene of bacillus cereus was cloned. its restriction map and the direction of transcription was determined. it was shown that the neutral proteinase gene could be expressed in bacillus cells. the thermostability of the product coded by the neutral proteinase gene and its natural analogue was explored. the obtained data indicate that the neutral proteinase of bacillus cereus is closely related to the enzyme of bacillus amyloliquefaciens by these parameters. it was found that ... | 1992 | 1339956 |
| organization and nucleotide sequence of the glutamine synthetase (glna) gene from lactobacillus delbrueckii subsp. bulgaricus. | a 3.3-kb bamhi fragment of lactobacillus delbrueckii subsp. bulgaricus dna was cloned and sequenced. it complements an escherichia coli glna deletion strain and hybridizes strongly to a dna containing the bacillus subtilis glna gene. dna sequence analysis of the l. delbrueckii subsp. bulgaricus dna showed it to contain the glna gene encoding class i glutamine synthetase, as judged by extensive homology with other prokaryotic glna genes. the sequence suggests that the enzyme encoded in this gene ... | 1992 | 1359838 |
| differential susceptibility of phosphatidylcholine small unilamellar vesicles to phospholipases a2, c and d in the presence of membrane active peptides. | activities of phospholipases c and d along with a2 were followed on egg phosphatidylcholine small unilamellar vesicles in the presence of membrane active peptides melittin, gramicidin s and alamethicin. decrease in the activity of phospholipase c and d and enhancement of phospholipases a2 activity suggest that these enzymes are sensitive to alterations in the lipid packing in the membranes in the presence of these peptides. phospholipase c and d, which have not been used to study peptide--membra ... | 1992 | 1370889 |
| comparative analysis of 23s ribosomal rna gene sequences of bacillus anthracis and emetic bacillus cereus determined by pcr-direct sequencing. | the primary structures of the 23s ribosomal rna genes of bacillus anthracis and an emetic strain of bacillus cereus were determined by direct sequencing of enzymatically amplified chromosomal dna. the 23s rrna gene sequences of b. anthracis and b. cereus were found to be almost identical and showed only two differences (a single nucleotide change, and a single base insertion in b. cereus). the feasibility of using pcr-direct sequencing for the rapid sequence determination of large-subunit rrna g ... | 1992 | 1381695 |
| a method for the determination of bacterial spore dna content based on isotopic labelling, spore germination and diphenylamine assay; ploidy of spores of several bacillus species. | a reliable method for measuring the spore dna content, based on radioactive dna labelling, spore germination in absence of dna replication and diphenylamine assay, was developed. the accuracy of the method, within 10-15%, is adequate for determining the number of chromosomes per spore, provided that the genome size is known. b subtilis spores were shown to be invariably monogenomic, while those of larger bacilli bacillus megaterium, bacillus cereus and bacillus thuringiensis, often, if not invar ... | 1992 | 1391052 |
| effect of side-chain amide thionation on turnover of beta-lactam substrates by beta-lactamases. further evidence on the question of side-chain hydrogen-bonding in catalysis. | two side-chain-thionated beta-lactams, a penicillin and a cephalosporin, have been prepared and found to be not significantly poorer as substrates of typical serine (classes a and c) beta-lactamases than are their oxo analogues. this result is interpreted to mean that any hydrogen-bonding site on these enzymes for the beta-lactam side-chain amide carbonyl group must be flexible and is more likely to be a passive rather than active or essential feature of the active site. previously, data from cr ... | 1992 | 1417747 |
| [fractal structure of microorganism colonies]. | 1992 | 1425170 | |
| [microbial contamination of twenty drugs of plant origin]. | twenty drugs of plant origin were analysed in order to determine the microbial contamination level. a light homogenizing method was used to avoid an increase in the antimicrobial capacity of certain drugs, which would falsify the analytical results. the total viable aerobic count (tvc) varied from 10(1) to 10(7) cfu/g and in 11 samples out of 21 was equal to or higher than 10(5) cfu/g. the number of fungi varied from one drug to another, but was generally lower than the aerobic tvc to the power ... | 1992 | 1438458 |
| mass sociogenic illness in a youth center. | in july, 1989, 63 (42%) of 150 children ages 4-14 years attending an outreach program at a youth center in florida, but no employees, developed acute and rapidly resolving upper gastrointestinal symptoms 2 to 40 minutes after a prepackaged lunch. all ill children were sent to 3 local hospital emergency departments for evaluation. however, clinical evaluation was normal for all. of 102 children who ate any prepackaged foods, 48 (47%) became ill compared to 1/19 (5%) for children who did not eat ( ... | 1992 | 1439062 |
| structural comparison suggests that thermolysin and related neutral proteases undergo hinge-bending motion during catalysis. | crystal structures are known for three members of the bacterial neutral protease family: thermolysin from bacillus thermoproteolyticus (tln), the neutral protease from bacillus cereus (neu), and the elastase of pseudomonas aeruginosa (pae), both in free and ligand-bound forms. each enzyme consists of an n-terminal and c-terminal domain with the active site formed at the junction of the two domains. comparison of the different molecules reveals that the structure within each domain is well conser ... | 1992 | 1445869 |
| changes in enzymatic and membrane-adsorbing activities of sphingomyelinase from bacillus cereus by modification with a polyethylene glycol derivative. | sphingomyelinase (smplc) from bacillus cereus was modified with a polyethylene glycol (peg) derivative, methoxypolyethylene glycol-succinimidyl succinate (ss-peg). the molecular weight of the ss-peg-modified smplc was calculated to be approx. 150 kda by gel-filtration whereas that of the native enzyme, was 25 kda. by this modification, the enzyme increased its thermostability and retained its hydrolytic activity toward 2-(n-hexadecanoylamino)-4-nitrophenylphosphocholine (hnp) and sphingomyelin ( ... | 1992 | 1446369 |
| cloning of a gene from bacillus cereus with homology to the mreb gene from escherichia coli. | we have cloned and sequenced a gene coding for a putative shape-determining protein (mreb) highly homologous to the mreb gene product of escherichia coli. the amino acid (aa) identity was 53% and the similarity 72%. the gene is expressed early in the logarithmic phase. the aa sequence comparison showed that the protein, like the e. coli mreb, has structural similarity to actin and heat-shock protein hsc70 encoded by a new super-gene family. | 1992 | 1452027 |
| ocular injuries caused by magpies. | this paper presents a series of six patients with ocular injuries resulting from magpie attacks. five cases involved children. in two cases the penetration was overlooked initially. in one case the keratitis was caused by bacillus cereus. full ophthalmic examination, including indirect ophthalmoscopy and microbiological studies, must be undertaken initially to identify unrecognised eye injuries and to prevent the possible sight-threatening complications of vitreal fibrosis with subsequent retina ... | 1992 | 1454001 |
| mechanisms of bacillus cereus enteropathy. | three strains of bacillus cereus isolated from sausages (salami and trekker, ranbac, ranchi) produced enterotoxin which caused vascular permeability in skin and haemorrhage in the ligated ileal loops of rabbits. histopathological studies revealed haemorrhage and congestion in submucosa, mononuclear cell infiltration in lamina propria and submucosa and villous atrophy. histochemical studies ruled out the effect on mitochondrial enzymes of intestinal epithelial cells. purified enterotoxin given in ... | 1992 | 1459602 |
| involvement of calcium in germination of coat-modified spores of bacillus cereus t. | the effect of calcium on germination of coat-modified bacillus cereus t spores was investigated. coat-modified spores produced either by chemical extraction (sds-dtt-treated spores) or by mutagenesis (10ld mutant spores) were unable to germinate in response to inosine. while sds-dtt-treated spores could germinate slowly in the presence of l-alanine, 10ld mutant spores could not germinate at all. the lost or reduced germinability of coat-modified spores was restored when exogenous ca2+ was supple ... | 1992 | 1461151 |
| prospective study of blood culture during transesophageal echocardiography. | to ascertain the incidence and significance of bacteremia associated with transesophageal echocardiography (tee), 132 consecutive patients (aged 17 to 73 years) free of apparent infection who were undergoing 135 transesophageal echocardiographic procedures from october 1990 to august 1991, were prospectively studied. for each procedure, two sets of blood cultures were obtained for culture 30 to 60 minutes before tee, immediately after, and 180 to 240 minutes after the procedure. for each blood c ... | 1992 | 1462911 |
| site-directed mutagenesis and substrate-induced inactivation of beta-lactamase i. | the substrate-induced inactivation of beta-lactamase i from bacillus cereus 569/h has been studied. both the wild-type enzyme and mutants have been used. the kinetics follow a branched pathway of the type recently analysed [waley (1991) biochem. j. 279, 87-94]. the substrate cloxacillin (a penicillin) formed an acyl-enzyme (characterized by m.s.), and it was probably the instability of this intermediate that brought about inactivation. a disulphide bond was introduced into beta-lactamase i (the ... | 1992 | 1471977 |
| studies on the atpase of bacillus cereus. | the membrane atpase (ec 3.6.1.3) of bacillus cereus was solubilized by a 'shock-wash' process and purified. the non-specific phosphatase contaminant was separated by glycerol density gradient centrifugation. the optimum temperature was 39.5 degrees c and the ph optimum at 7.5. on sds-polyacrylamide gel electrophoresis two classes of subunits were observed in equal proportions with molecular weights of 70 k and 83 k. the effect of various compounds on the enzymatic activity was studied. the enzym ... | 1992 | 1473262 |
| bacillus cereus meningitis in two neurosurgical patients: an investigation into the source of the organism. | two patients developed bacillus cereus meningitis following neurosurgery. during the subsequent investigation into the source of the organism, linen was discovered to be heavily contaminated with b. cereus. no other prolific source of the organism was found. it seems probable that lint from contaminated fabric was the vehicle of transmission of the organism during extended surgery. linen should be considered as a possible source of b. cereus infection. | 1992 | 1474265 |
| [studies of cockroachcidal toxin]. | a cockroachcidal bacterial isolate cw-w-90-3 was selected by egg yolk agar plate. the isolate produced phospholipase c (plc) which was pathogenic to the nymph of cockroach. the conditions for production of high level plc indicated that using lb medium supplement tween-80 or minimal medium could effectively increase the activity of plc. the optical phase for production of plc was in the period of 12-18 hours and below ph8.0. the activity of plc was reduced along with the culture time until 48 hou ... | 1992 | 1481534 |
| media for the detection and enumeration of bacillus cereus in foods: a review. | bacillus cereus is an established cause of food poisoning in addition to being a troublesome and persistent contaminant, responsible for a variety of spoilage defects in processed foods and dairy products. a range of diagnostic and selective media has been developed to facilitate the detection and enumeration of b. cereus in routine surveillance situations and food poisoning investigations. these media are reviewed with respect to the selective and diagnostic systems they employ, their ability t ... | 1992 | 1486027 |
| mass production of sphingomyelinase of bacillus cereus by a protein-hyperproducing strain, bacillus brevis 47, and its purification. | sphingomyelinase (sphingomyelin cholinephosphohydrolase) [ec 3.1.4.12] of bacillus cereus was overproduced in a protein-hyperproducing strain, b. brevis 47, by cloning the gene into an expression vector pnu211, which has been developed to express a foreign gene utilizing a promoter and a signal sequence of an outer cell wall protein gene. from 1 liter of culture, about 10 mg of protein was purified to near-homogeneity by two steps of column chromatography; this is almost 500 times higher product ... | 1992 | 1491003 |
| relationship between selected bacteria and the growth of immature house flies, musca domestica, in an axenic test system. | to investigate the relationship between immature (maggot) house flies, musca domestica, and bacteria, we compared the development of sterile first-instar maggots in each of 10 pure blood agar cultures of bacteria with growth on sterile blood agar (negative control) and on standard house fly rearing medium (positive control). nine species of bacteria representing gram-negative and gram-positive rods, coccoid, and micrococcoid cell types supported house fly growth on blood agar. one bacterium, a s ... | 1992 | 1495035 |
| the role of the pro-sequence in the processing and secretion of the thermolysin-like neutral protease from bacillus cereus. | the bacillus cereus cnp gene coding for the thermolysin-like neutral protease (tnp) has been cloned, sequenced, and expressed in bacillus subtilis. the protease is first produced as a pre-pro-protein (m(r) = 61,000); the pro-peptide is approximately two-thirds of the size of the mature protein. the pro-sequence has been compared with those of six other tnps, and significant homologies have been found. additionally, the tnp pro-sequences are shown to be homologous to the pro-sequence of pseudomon ... | 1992 | 1495388 |
| [meningitis caused by bacillus cereus]. | 1992 | 1498184 | |
| early diagnosis of infectious keratitis with in vivo real time confocal microscopy. | the tandem scanning confocal microscope (tsm) was adapted for in vivo examination of the cornea in rabbits with experimental bacterial and fungal keratitis. compared to slit lamp biomicroscopy, the tsm provides superior lateral and axial resolution and serial optical sectioning capability, which may be useful for identification of corneal pathogens in the early stages of infection. we used the tsm to examine normal rabbit eyes infected with bacteria (bacillus cereus) and a filamentous fungus (as ... | 1992 | 1499129 |
| [determining the sensitivity of anthrax bacteria to antibiotics for its differentiation from the antibiotic sensitivity of spore-forming saprophytes]. | 1992 | 1514849 | |
| monoclonal antibody-beta-lactamase conjugates for the activation of a cephalosporin mustard prodrug. | cephalosporin mustard (cm) was designed as an anticancer prodrug that could be activated in a site-specific manner by monoclonal antibody-beta-lactamase conjugates targeted to antigens present on tumor cell surfaces. purified beta-lactamases from bacillus cereus (bc beta l) and escherichia coli (ec beta l) catalyzed the release of phenylenediamine mustard (pdm) from cm through a fragmentation reaction which occurs after the beta-lactam ring of cm is hydrolyzed. the km and vmax values were 5.7 mi ... | 1992 | 1515470 |
| evaluation of the effect of 3 probiotics on experimental escherichia coli enterotoxaemia in weaned piglets. | in this report the efficacy of 3 different probiotics (bacillus cereus "toyoi", lactobacillus spp. and streptococcus faecium) was investigated. they were supplemented in the food of recently weaned piglets that were orally infected with e. coli o141 k85ab. supplementation could not prevent mortality and clinical symptoms nor reduce the faecal excretion of hemolytic e. coli. the possible explanations for the unsatisfying results are various. | 1992 | 1519408 |
| purification of an enterotoxin produced by bacillus cereus by immunoaffinity chromatography using a monoclonal antibody. | a murine monoclonal antibody (mab) with high reactivity to an enterotoxin produced by bacillus cereus was used to prepare an immunoadsorbent for purification of the enterotoxin. by immunoaffinity chromatography using the immunoadsorbent, approximately 25% of crude enterotoxin applied was recovered in the eluate. the purified enterotoxin was found to be electrophoretically and antigenically homogeneous. it also showed vascular permeability activity and mouse lethality, and caused fluid accumulati ... | 1992 | 1521189 |
| effect of 22r-hydroxycholesterol on the action of sphingomyelinase from bacillus cereus toward bovine erythrocytes. | the incorporation of 22r-hydroxycholesterol [(22r)-5-cholestene-3 beta,22-diol] into the bovine erythrocyte membranes remarkably enhanced the degradation of sphingomyelin in erythrocyte membranes by the action of sphingomyelinase from bacillus cereus, causing much faster hemolysis of erythrocytes. the stimulative effect of 22r-hydroxycholesterol on the breakdown of sphingomyelin was maximal in the presence of mg2+. on the other hand, in spite of the presence of 22r-hydroxycholesterol, the breakd ... | 1992 | 1523674 |
| structure and mechanism of alkaline phosphatase. | alkaline phosphatase was the first zinc enzyme to be discovered in which three closely spaced metal ions (two zn ions and one mg ion) are present at the active center. zn ions at all three sites also produce a maximally active enzyme. these metal ions have center-to-center distances of 3.9 a (zn1-zn2), 4.9 a (zn2-mg3), and 7.1 a (zn1-mg3). despite the close packing of these metal centers, only one bridging ligand, the carboxyl of asp51, bridges zn2 and mg3. a crystal structure at 2.0-a resolutio ... | 1992 | 1525473 |
| nucleotide sequence and deduced functions of a set of cotranscribed genes of streptomyces coelicolor a3(2) including the polyketide synthase for the antibiotic actinorhodin. | a 5.3-kb region of the streptomyces coelicolor actinorhodin gene cluster, including the genes for polyketide biosynthesis, was sequenced. six identified open reading frames (orf1-6) were related to genetically characterized mutations of classes acti, vii, iv, and vb by complementation analysis. orf1-6 run divergently from the adjacent actiii gene, which encodes the polyketide synthase (pks) ketoreductase, and appear to form an operon. the deduced gene products of orf1-3 are similar to fatty acid ... | 1992 | 1527048 |
| interaction of energized bacteria cells with particles of colloidal gold: peculiarities and kinetic model of the process. | it is found that the cells of bacillus cereus b-4368 at energized state can concentrate the colloidal gold particles on their surface. it is shown that the process depends on metabolic reactions proceeding on the plasma membrane. the inhibitory analysis permits to suppose that the metal concentration is due to the functioning of atp-dependent generator of the transmembrane potential, apparently, of proton atpase. kinetic characteristics of the process show the presence of an intermediate state i ... | 1992 | 1532513 |
| assignment of bacillus thermoamyloliquefaciens kp1071 alpha-glucosidase i to an exo-alpha-1,4-glucosidase, and its striking similarity to bacillary oligo-1,6-glucosidases in n-terminal sequence and in structural parameters calculated from the amino acid composition. | alpha-glucosidase i of bacillus thermoamyloliquefaciens kp1071 (ferm p8477, facultative thermophile) was purified to homogeneity. the relative molecular mass was estimated to be 62,000 da. from its catalytic properties, the enzyme has been assigned to an exo-alpha-1,4-glucosidase. the enzyme shares its antigenic groups in part with bacillus stearothermophilus atcc12016 (obligate thermophile) exo-alpha-1,4-glucosidase. these exo-alpha-1,4-glucosidases strikingly resemble oligo-1,6-glucosidases fr ... | 1992 | 1555585 |
| adp-ribosylation of small gtp-binding proteins by bacillus cereus. | a human pathogenic strain of bacillus cereus produces an exoenzyme which selectively adp-ribosylates 20-25 kda gtp-binding proteins in platelet membranes. pre-adp-ribosylation of rho proteins of human platelet membranes with clostridium botulinum exoenzyme c3 or clostridium limosum exoenzyme inhibits subsequent adp-ribosylation by the exoenzyme from b. cereus indicating similar substrate specificity of the transferases. the adp-ribosyltransferase from b. cereus reveals no immunological cross-rea ... | 1992 | 1567406 |
| antimicrobial properties of secretions from the metapleural glands of myrmecia gulosa (the australian bull ant). | myrmecia gulosa (australian bull ant) produce secretions from their metapleural exocrine glands which have broad spectrum antimicrobial properties. such secretions are probably of importance in disease control in bull ant communities. these antimicrobial secretions are stable at 100 degrees c, resistant to proteolytic enzymes and are active over a wide ph range. of the organisms tested only endospores of bacillus cereus were found to be resistant. the antimicrobial agent(s) are absorbed by cells ... | 1992 | 1568945 |
| synthetic phospholipids as substrates for phospholipase c from bacillus cereus. | the substrate requirement of phospholipids for hydrolysis with phospholipase c from bacillus cereus was studied with synthetic lipids well-defined in structure and configuration. for optimal activity, the glycerol molecule must contain three substituents: phosphocholine in sn-3-, an ester bond in sn-2- and an ether- or ester bond in sn-1-position. the length of the ester or ether chains is of minor importance. any deviation from these structural requirements results in a large decrease in the hy ... | 1992 | 1571359 |
| formycins a and b and some analogues: selective inhibitors of bacterial (escherichia coli) purine nucleoside phosphorylase. | formycin b (fb), a moderate inhibitor (ki approximately 100 microm) of mammalian purine nucleoside phosphorylase (pnp), and formycin a (fa), which is totally inactive vs. the mammalian enzyme, are both effective inhibitors of the bacterial (escherichia coli) enzyme (ki approximately 5 microm). examination of a series of n-methyl analogues of fa and fb led to the finding that n(6)-methyl-fa, virtually inactive vs. the mammalian enzyme, is the most potent inhibitor of e. coli purine nucleoside pho ... | 1992 | 1576149 |
| bacillus cereus endocarditis: report of a case and review. | bacillus cereus is a ubiquitous organism that infrequently causes serious infections. we report a patient with b. cereus endocarditis involving a mechanical aortic valve. data for 10 cases of b. cereus endocarditis reported in the literature are summarized. b. cereus is resistant to many commonly used antibiotics, a finding that has clinical significance for empirical antibiotic selection in patients with suspected endocarditis. infection in patients with valvular heart disease in the few cases ... | 1992 | 1576291 |
| toxic properties of bacillus cereus strains isolated from different foodstuffs. | toxic activity of 89 culture filtrates of bacillus cereus strains were examined. from the total number of the filtrates tested, 3 were found to cause fluid accumulation in the ligated rabbit ileal loop. 71 samples caused a vascular permeability increase in the rabbit skin corresponding to an intermediate or strong toxin production. 59 of the 89 culture filtrates were cytotoxic on vero cells, 31 had a lethal effect, 81 induced hemolysis of 0.5% rabbit erythrocytes and 55 tested samples showed lec ... | 1992 | 1576401 |
| substrate-enzyme interactions and catalytic mechanism in phospholipase c: a molecular modeling study using the grid program. | based on the high-resolution x-ray crystallographic structure of phospholipase c from bacillus cereus, the orientation of the phosphatidylcholine substrate in the active site of the enzyme is proposed. the proposal is based on extensive calculations using the grid program and molecular mechanics geometry relaxations. the substrate model has been constructed by successively placing phosphate, choline and diacylglycerol moieties in the positions indicated from grid calculations. on the basis of th ... | 1992 | 1579567 |
| role of spore coats in the germinative response of bacillus cereus to adenosine and its analogues. | spores of the strain ncib 8122 of bacillus cereus have been depleted of coats by treatment with 0.1% sodium dodecyl sulfate--200 mm 2-mercaptoethanol--0.5 m nacl (ph 9.6). the coat-depleted spores did not show any decrease in viability, heat resistance, refractility, dipicolinic acid content, or specific activities of several protoplastic enzymes. the germinative response of the coat-depleted spores to adenosine and several analogues thereof was found qualitatively similar to that obtained with ... | 1992 | 1581864 |
| a type-iii dna restriction and modification system in bacillus cereus? | 1992 | 1587478 | |
| physical maps of the genomes of three bacillus cereus strains. | noti restriction maps of the chromosomes from bacillus cereus atcc 10876, atcc 11778, and the b. cereus type strain atcc 14579 have been established and compared with the previously established map of b. cereus atcc 10987. between 10 and 14 noti fragments were observed, ranging from 15 to 1,300 kb, in digests of dna from the various strains. the sizes of the genomes varied between 5.4 and 6.3 mb. the maps were constructed by hybridization of 42 random probes, prepared from b. cereus atcc 10987 l ... | 1992 | 1592826 |
| crystal structures of phosphate, iodide and iodate-inhibited phospholipase c from bacillus cereus and structural investigations of the binding of reaction products and a substrate analogue. | the crystal structure of the complex formed between phospholipase c (plc) from bacillus cereus and inorganic phosphate (pi), which is an inhibitor, has been determined and refined to 2.1 a resolution. the final r-factor is 19.7%. we have also studied the binding of two other inhibitors, iodide and iodate, to plc. x-ray data for these two complexes were collected to 2.8 a resolution during the search for heavy-atom derivatives. a series of screening experiments where plc crystals have been treate ... | 1992 | 1593635 |
| investigation of the substrate binding and catalytic groups of the p-c bond cleaving enzyme, phosphonoacetaldehyde hydrolase. | kinetic studies with substrate analogs and group-directed chemical modification agents were carried out for the purpose of identifying the enzyme-substrate interactions required for phosphonoacetaldehyde (p-ald) binding and catalyzed hydrolysis by p-ald hydrolase (phosphonatase). malonic semialdehyde (ki = 1.6 mm), phosphonoacetate (ki = 10 mm), phosphonoethanol (ki = 10 mm), and fluorophosphate (ki = 20 mm) were found to be competitive inhibitors of the enzyme but not substrates. thiophosphonoa ... | 1992 | 1605625 |
| bacteriological quality of infant feeding bottle-contents and teats in addis abeba, ethiopia. | bacteriological quality of 244 infant feeding-bottles and 61 teats were studied from january 1989 to november 1989. samples were collected from feeding-bottles of babies who were brought by nursing mothers to clinics and hospitals for varying complaints. of the 244 bottle-content samples, 144 (59%) harboured more than 2 x 10(6) colonies of bacteria per ml. of samples. the predominant bacterial isolates both from bottle-contents and teat-swabs were the coliform group of bacteria, such as e. coli, ... | 1992 | 1606947 |
| isolation and characterisation of restriction endonuclease bcuai from bacillus cereus a. | 1992 | 1614885 | |
| rapid identification of viable bacterial spores using a fluorescence method. | a method has been devised to differentiate viable and nonviable bacterial spores. "germination-like" changes are initiated in spores with performic acid and lysozyme. the germinated spores are stained with aqueous acridine orange, a fluorescent dye. nonviable spores fluoresce lemon-green and viable spores orange-red. it is proposed that with the use of a membrane filter resistant to performic acid and lysozyme, the method may be used for spore enumeration in foods in about 4 hr compared to conve ... | 1992 | 1616999 |
| nucleotide sequence and phylogeny of the tet(l) tetracycline resistance determinant encoded by plasmid pste1 from staphylococcus hyicus. | the nucleotide sequence of the tetracycline resistance (tet) gene and its regulatory region, encoded by the plasmid pste1 from staphylococcus hyicus, was determined. the tet gene was inducible by tetracycline and encoded a hydrophobic protein of 458 amino acids. comparisons between the predicted amino acid sequences of the pste1-encoded tet from s. hyicus and the previously sequenced tet k variants from staphylococcus aureus, tet l variants from bacillus cereus, bacillus stearothermophilus, and ... | 1992 | 1622166 |
| campylobacter jejuni non-culturable coccoid cells. | the behaviour of campylobacter jejuni in the environment is poorly documented. rapid loss of viability on culture media is reported. this phenomenon is associated with the development of so-called coccoid cells. it has been suggested that these cells can be infective to animals and man. results obtained with atp-measurements of coccoid cells and direct viable count (dvc) support this hypothesis. introduction of coccoid cells into simulated gastric, ileal and colon environments did not result in ... | 1992 | 1622752 |
| tracking laboratory contamination by using a bacillus cereus pseudoepidemic as an example. | from 1 march to 31 may 1990, bacillus cereus was recovered from 24 of 5,534 (0.49%) blood cultures and 22 of 1,088 (2.02%) other body fluid cultures. the rarity of this organism as a pathogen and comparison with previous baseline rates led to the conclusion that it was a pseudoepidemic involving some form of culture contamination. generalized precautions taken without specific knowledge of the contaminant source reduced the recovery rate of the organism. recovery rates for the organism returned ... | 1992 | 1624565 |
| transfer of a chloroplast-bound precursor protein into the translocation apparatus is impaired after phospholipase c treatment. | we have studied the influence of phospholipase c treatment of intact purified chloroplast on the translocation of a plastid destined precursor protein. under standard import conditions, i.e. in the light in the presence of 2 mm atp translocation was completely abolished but binding was observed at slightly elevated levels. an experimental regime which allowed binding but not import of the precursor protein, i.e. in the dark in the presence of 10 microm atp, demonstrated that translocation interm ... | 1992 | 1628746 |
| quantification of distinct molecular species of the 2-lyso metabolite of platelet-activating factor by gas chromatography-negative-ion chemical ionization mass spectrometry. | the biological activity of platelet-activating factor (paf) is comprised by a few molecular species of phosphatidylcholine which contain a fatty alcohol connected by an ether linkage to the sn-1 position of the glycerol backbone and an acetate ester at the sn-2 position. the various molecular species of paf differ in chain length and degree of unsaturation in the fatty alcohol residue side-chain. paf is rapidly hydrolyzed to lyso-paf by an acetylhydrolase enzyme which is quite active in a number ... | 1992 | 1629294 |
| differential scanning calorimetric study of the thermal unfolding of beta-lactamase i from bacillus cereus. | the irreversible thermal unfolding of the class a beta-lactamase i from bacillus cereus has been investigated at ph 7.0, using differential scanning calorimetry (dsc) and inactivation kinetic techniques. dsc transitions showed a single peak with a denaturation enthalpy of 646 kj.mol-1 and were moderately scan rate dependent, suggesting that the process was partially kinetically controlled. the inactivation kinetics at constant temperature showed that the irreversible denaturation of the enzyme o ... | 1992 | 1633173 |
| the structure of neutral protease from bacillus cereus at 0.2-nm resolution. | the crystal structure of the neutral protease from bacillus cereus has been refined to an r factor of 17.5% at 0.2-nm resolution. the enzyme, an extracellular metalloendopeptidase, consists of two domains and binds one zinc and four calcium ions. the structure is very similar to that of thermolysin, with which the enzyme shares 73% amino-acid sequence identity. the active-site cleft between the two domains is wider in neutral protease than in thermolysin. this suggests the presence of a flexible ... | 1992 | 1633827 |
| a single mode fibre-optic evanescent wave biosensor. | this paper reports experimental developments in the construction and operation of a single-mode fibre-optic evanescent wave biosensor using an exposed core silica single-mode fibre embedded in a silica block. the device was able to monitor the concentration of a blue dye, procion blue mx-g, in overlayers of various refractive indices. the practicality of such a biosensor has been demonstrated with a colorimetric enzyme assay system. penicillin g in the 0-0.4 mm concentration range was monitored ... | 1992 | 1637526 |
| an outbreak of bacillus cereus food poisoning--are caterers supervised sufficiently. | bacillus cereus is an uncommonly reported cause of foodborne illness in the united states. in may 1989, an outbreak of b. cereus gastroenteritis occurred among 140 guests who had attended a catered wedding reception in napa, ca. investigation established cornish game hens served at the event as the vehicle for disease transmission (or = 29, p = 0.0001). although the spores of b. cereus are ubiquitous, large numbers of toxin-producing organisms (more than 10(5) per gram of food) are required for ... | 1992 | 1641447 |
| production of the vacuolation factor of bacillus cereus isolated from vomiting-type food poisoning. | vacuole response in hep-2 cells was induced with culture supernatants of bacillus cereus strains isolated from outbreaks of vomiting- and diarrheal-type food poisoning grown in rice flour and laboratory media. high vacuole response was obtained with culture supernatants of b. cereus strains isolated from vomiting-type food poisoning grown in cooked rice suspension or on a cooked rice plate, whereas no response was obtained with those of the same strains grown in brain heart infusion and trypto-s ... | 1992 | 1643168 |
| an investigation of dye reduction by food-borne bacteria. | the rates of reduction of seven redox dyes by 13 bacterial strains were measured and found to vary greatly between different bacterium/dye combinations. phenazine ethosulphate and toluidine blue were the most rapidly reduced dyes by the majority of bacteria and resorufin and 2-hydroxy-1,4-naphthoquinone were reduced slowly, if at all. there was also considerable variation in the rates of reduction with any single dye/organism combination. glucose stimulated the rates of endogenous dye reduction ... | 1992 | 1644703 |
| distribution, frequency, and diversity of bacillus thuringiensis in an animal feed mill. | bacillus thuringiensis was isolated from 36 of 50 residue samples obtained from an animal feed mill (a stored-product environment). of 710 selected colonies having bacillus cereus-b. thuringiensis morphology isolated from the samples, 477 were classified as b. thuringiensis because of production of parasporal delta-endotoxin crystals. there was a diverse population of b. thuringiensis, as revealed by differentiation of the isolates into 36 subgroups by using (i) their spectra of toxicity to the ... | 1992 | 16348699 |
| inhibited growth of common enteropathogenic bacteria in lactic-fermented cereal gruels. | a natural lactic fermentation of mixtures of water and whole flour of either maize or high-tannin sorghum was obtained either before or after cooking to a weaning gruel: the preparations had a final ph of about 3.8 (range 3.67 to 4.00) and a ratio of lactic acid to acetic acid of 9∶1 (w/w). the growth of added (about 10(7) c.f.u./g gruel) gram-negative intestinal pathogenic bacteria, enterotoxigenicescherichia coli, campylobacter jejuni, shigella flexneri andsalmonella typhimurium, was strongly ... | 1992 | 24425608 |
| interactions of bacteria and microflagellates in sequencing batch reactors exhibiting enhanced mineralization of toxic organic chemicals. | community level interactions were studied in non-axenic sequencing batch reactors (sbrs) being used to treat 2,4-dinitrophenol (dnp). increasing the influent dnp concentrations from 1 to 10 µg ml(-1) eliminated large predatory organisms such as rotifers and ciliated protozoa from the sbrs. under steady-state conditions at a dnp concentration of 10 µg ml(-1), supplemental additions of glucose enhanced dnp degradation and led to the establishment of a microbial community consisting of five species ... | 1992 | 24192859 |
| [tyrosol--the autoregulatory d1 factor of the yeast saccharomyces cerevisiae]. | the autoregulatory d1 factor of the yeast, saccharomyces cerevisiae, that induces the transition of vegetative cells into refractory resting forms, has been isolated from the cell-free culture medium as an individual crystalline compound. it has been shown to be 2-(4-hydroxyphenyl)ethane-1-ol which is also known as tyrosol. when added to the producer culture at 5-15 microm concentration, tyrosol stimulated the endogenous respiration of cells, but inhibited at 20-80 microm concentration. at 200-8 ... | 1993 | 7506347 |
| a study of purified pyocyanine produced by pseudomonas aeruginosa: properties and grouping. | a study of more than one thousand strains of pseudomonas aeruginosa was performed. these were collected from different sources, i.e. humans, animals, environment and food. cumulative results revealed the existence of three different groups of pyocyanine. the biological importance of grouping this pigment is due to its activity against other bacteria in comparison with other antibiotics such as cyanomycin produced by streptomyces cyanoflavus. no such grouping or any other classification was found ... | 1993 | 7516618 |
| phase partitioning detects differences between phospholipase-released forms of alkaline phosphatase--a gpi-linked protein. | a number of enzymes are known to release alkaline phosphatase and other glycan phosphatidylinositol-anchored proteins from membrane surfaces. we describe a novel approach to detect and measure these activities by partitioning in aqueous phase systems. the procedures avoid the complications of micelle-formation involving hydrophobic molecules that may arise with detergent-based partition systems and can clearly distinguish between inositol-specific phospholipase c and d activities. | 1993 | 7678987 |
| protein kinase c zeta isoform is critical for mitogenic signal transduction. | the requirement of protein kinase c zeta (zeta pkc) for maturation of x. laevis oocytes in response to insulin, p21ras, and phosphatidylcholine-hydrolyzing phospholipase c has recently been shown. here we present experimental evidence demonstrating that activation of zeta pkc is not only necessary but also sufficient by itself to activate maturation in oocytes and to produce deregulation of growth control in mouse fibroblasts. furthermore, by using a dominant kinase-defective mutant of zeta pkc, ... | 1993 | 7688666 |