Publications

tumor necrosis factor is partly responsible for the differentiation of mouse myeloid leukemia cells.	when mouse myeloid leukemia m1 cells were incubated with 0.1% tns(serum containing tumor necrosis factor, tnf), they were induced to differentiate into macrophage-like cells within 14 h. the stability of the differentiation activity to heat treatment was very similar to that of the cytotoxicity of tnf for l929 cells. the differentiation activity could not be separated from the tnf activity by sequential chromatography procedures using deae-sephadex and sephacryl s-200 or by isoelectric focusing. ...	1987	3440028
[use of erythrocyte diagnostic agents in the universal system of serological reactions].	a method of parallel serologic studies of the material for the detection of specific antigen or antibodies is proposed. this method is not inferior to the known systems of serologic tests (passive hemagglutination-antigen neutralization tests, passive hemagglutination--antibody neutralization tests, etc) in sensitivity and economy, but exceeds all of them in informative value. the method has been tested both experimentally and in the epizootic study of plague infection used as a model. a serolog ...	1987	3445733
immunohistochemical localization of metallothionein in cell nucleus and cytoplasm of fetal human liver and kidney and its changes during development.	the distribution of metallothionein (mt) during human development was investigated using both immunohistochemical and biochemical methods. the level of mt in the fetal liver was higher than the adult liver levels. higher levels of zinc (zn) and copper (cu) were also detected in the fetal liver compared to the adult liver. although cadmium (cd) was present in detectable levels in the human adult liver, none was detected in the human fetal liver. mt was localized in the nucleus and the cytoplasm o ...	1987	3324023
[serological reaction systems using enzyme-labelled immunospecific reagents].	different serological test systems, based on the use of enzyme-labeled immunospecific reagents and intended for testing the material under study for the presence of yersinia pestis capsular antigen and antibodies to it, are described. comparative data on the evaluation of their sensitivity to the antigen and antibodies to it in different schemes of enzyme immunoassays (eia) are presented. as shown in this investigation, eia systems for the detection of the antigen and antibodies to it can compri ...	1987	3318229
[structural and functional properties and the biological activity of yersinia pestis capsular antigen, murine toxin and endotoxin].		1987	3300112
[dependence of the antimicrobial effect on the antibiotic concentration in modelling pharmacokinetic profiles in vitro: a comparison of 2 regimens for the single intravenous administration of sisomicin].	kinetics of sisomicin antimicrobial effect on e. coli was studied in an in vitro dynamic model. pharmacokinetic profiles observed in blood of humans treated with different polar dosing schedules i. e. bolus administration and continuous infusion were simulated. the first set of the experiments included simulating the profiles observed at short-term maintenance (0.25-20 min) of relatively high antibiotic levels after bolus administration (cmax = 0.125-10 micrograms/ml). the second set included si ...	1987	3324953
[dependence of the antimicrobial effect on the antibiotic concentration in modelling pharmacokinetic profiles in vitro: a comparison of 2 regimens for the single intravenous administration of sisomicin].	kinetics of sisomicin antimicrobial effect on e. coli was studied in an in vitro dynamic model. pharmacokinetic profiles observed in blood of humans treated with different polar dosing schedules i. e. bolus administration and continuous infusion were simulated. the first set of the experiments included simulating the profiles observed at short-term maintenance (0.25-20 min) of relatively high antibiotic levels after bolus administration (cmax = 0.125-10 micrograms/ml). the second set included si ...	1987	3324953
[chromosome stability in saccharomycete yeasts].	mutants with high instability of chromosome iii designated chl+ (chromosome loss) were obtained after irradiation with uv the z4221-3c1 haploid disomic for chromosome iii. the chl+ mutants can be divided into two classes: 1) cl2, cl3, cl7, cl9, cl11, cl12, cl13 with elevated level of spontaneous inter- and intragenic recombination; 2) cl4, cl8 which unstable maintenance of chromosome iii not accompanied with elevation of mitotic recombination frequency. the cl4 and cl8 mutants also reveal, in co ...	1987	3326785
fetal heart rate, arterial pressure, and blood volume responses to cortisol infusion.	the purpose of this study was to determine whether physiological amounts of cortisol affect the fetal cardiovascular system. cortisol (4 micrograms/min) or the vehicle was infused intravenously for 5 h into six chronically catheterized sheep fetuses at 127-143 days gestation (term = 145-150 days). in the cortisol-infused animals, plasma cortisol concentration increased from 2.0 +/- 0.6 (se) to 8.3 +/- 0.9 ng/ml. there was a concomitant decrease in fetal heart rate of 38 beats/min (p less than 1 ...	1987	3322047
[frequency of appearance of persistent bvd virus infections and their effects on the cattle population].		1987	3322777
mobilization of intracellular calcium by methacholine and inositol 1,4,5-trisphosphate in rat parotid acinar cells.	in the rat parotid acinar cell, methacholine caused an increase in [ca2+]i as determined by quin-2 fluorescence. the increase in [ca2+]i was initially independent of, and subsequently dependent on, the presence of extracellular ca2+, indicating mobilization of intracellular ca2+, as well as activation of ca2+ entry. methacholine mobilization of the internal ca2+ pool and stimulation of the initial transient phase of k+ efflux have similar concentration dependencies; the ec50 value for ca2+ mobil ...	1987	3305630
a simple method to eliminate the antigenicity of surface class i mhc molecules from the membrane of viable cells by acid treatment at ph 3.	we describe here a simple, reproducible method which specifically eliminates the antigenicity of surface class i major histocompatibility complex (mhc) molecules by acid treatment at ph 3 from the membrane of viable cells. when fresh mononuclear cells (mnc) or established cultured cell lines were treated at 4 degrees c for 2 min with citric acid buffer at ph 3 containing 1% bovine serum albumin, the antigenicity of class i mhc molecules, but not those of class ii mhc and the other non-mhc antige ...	1987	3298442
epinephrine, glucose, and lactate infusion in exercising adrenodemedullated rats.	the purpose of this study was to determine the metabolic function of the marked increase in plasma epinephrine which occurs in fasted rats during treadmill exercise. fasted adrenodemedullated (adm) and sham-operated (sham) rats were run on a rodent treadmill (21 m/min, 15% grade) for 30 min or until exhaustion. adm rats were infused with saline, epinephrine, glucose, or lactate during the exercise bouts. adm saline-infused rats showed markedly reduced endurance, hypoglycemia, elevated plasma ins ...	1987	3298192
antigenic relatedness between glycoproteins of human respiratory syncytial virus subgroups a and b: evaluation of the contributions of f and g glycoproteins to immunity.	the degree of antigenic relatedness between human respiratory syncytial virus (rsv) subgroups a and b was estimated from antibody responses induced in cotton rats by respiratory tract infection with rsv. glycoprotein-specific enzyme-linked immunosorbent assays of antibody responses induced by rsv infection demonstrated that the f glycoproteins of subgroups a and b were antigenically closely related (relatedness, r approximately 50%), whereas the g glycoproteins were only distantly related (r app ...	1987	3305988
bubonic plague.		1987	3308795
[a personal view of the history of the genus yersinia].	the first recorded experience australia had of the genus yersinia was the arrival in 1889 of a french expedition led by pasteur's nephew, dr. adrien loir. at that time australia was in the grips of an epidemic of rabbits, and loir's purpose was to eradicate the rabbits by means of fowl plague (pasteurella multocida). sadly, bureaucratic and political obstacles prevailed, and loir was never granted permission to release his biological control agent. alexander yersin had been tempted to join loir' ...	1987	3311629
measurement of regional myocardial perfusion and mass by subselective hydrogen infusion and washout techniques: a validation study.	a technique was developed for measuring regional myocardial perfusion by intracoronary infusions of hydrogen (h2)-saturated saline. h2 concentration was detected during washout in the pulmonary artery by means of the voltage response of a platinum-tipped electrode. regional myocardial perfusion was calculated from the h2 exponential desaturation curve according to the kety-schmidt principle. in 16 anesthetized open-chest dogs, validation of this technique was performed at baseline, reduced (sten ...	1987	3315296
diisopropanolnitrosamine (dipn) induced rat thyroid lesions. iii. an immunohistochemical study on their functional aspects.	the functional aspects of dipn induced rat thyroid lesions classified as in our previous studies were analyzed by immunohistochemical detection of tg, t4, and t3 in serial or semi-serial paraffin sections. the type 1 lesions, i.e., foci of cellular alteration of individual follicles, showed the same pattern of immunostaining as seen in the surrounding hyperplastic follicles and in normal controls. on the other hand, the type 3 lesions, i.e., carcinomas, were mostly negative for t4 and t3 with on ...	1987	3318273
radioimaging of malignant melanoma xenografts in nude mice using 111in-labeled monoclonal antibody against human malignant melanoma.		1987	3312335
concentrations of antibodies in paired maternal and infant sera: relationship to igg subclass.	previous studies comparing igg subclass concentrations in cord and maternal sera have indicated that igg1 is transported across the placenta to a greater extent than is igg2. the purpose of our study was to examine the relationship between the transport of igg1 and igg2 and the transport of specific antibodies that are relatively restricted to a particular subclass, either igg1 or igg2. the concentrations of total serum igg1 and igg2 and those of igg-anti-tetanus toxoid (tt) and anti-group a str ...	1987	3312554
measurement of regional myocardial perfusion and mass by subselective hydrogen infusion and washout techniques: a validation study.	a technique was developed for measuring regional myocardial perfusion by intracoronary infusions of hydrogen (h2)-saturated saline. h2 concentration was detected during washout in the pulmonary artery by means of the voltage response of a platinum-tipped electrode. regional myocardial perfusion was calculated from the h2 exponential desaturation curve according to the kety-schmidt principle. in 16 anesthetized open-chest dogs, validation of this technique was performed at baseline, reduced (sten ...	1987	3315296
evidence that dna involved in the expression of nodulation (nod) genes in rhizobium binds to the product of the regulatory gene nodd.	in rhizobium leguminosarum biovar viciae, the regulatory nodulation nodd gene has at least two functions. it constitutively represses its own transcription and in the presence of inducer flavonoid molecules, it activates the expression of two other nod gene transcriptional units, nodabcij and nodfe. upstream of noda and nodf is a conserved sequence, the nod box, which has been implicated in nodd-mediated transcriptional activation of these genes. dna fragments spanning the nod boxes that precede ...	1987	3320955
the aetiology of jaundice in adult patients at port moresby general hospital, papua new guinea.		1987	3321750
cellular and subcellular distribution of exogenously administered renal renin in rat liver and kidney.	using highly purified 125i-labeled rat renal renin, we have demonstrated that both the liver and kidney are responsible for clearance of renal renin. in the present study, light and electron microscope autoradiography of intravenously administered 125i-labeled rat renal renin was performed in rat liver and kidney to observe the cellular and subcellular distribution of the renin. fifteen and 60 min after the injection, the liver and kidneys were removed and fixed with 0.1 m sodium phosphate buffe ...	1987	3322039
nonenzymatic oxidative cleavage of peptide bonds in apoprotein b-100.	incubation of low density lipoprotein (ldl) with endothelial cells converts it to a form that is avidly degraded by macrophages via the acetyl ldl receptor. this modification has previously been shown to be accompanied by extensive breakdown of the major ldl protein (apob-100) to smaller peptides. apob-100 is known to undergo partial degradation during isolation and purification which is commonly attributed to proteolytic enzymes derived from plasma or to contaminant bacteria. in the present stu ...	1987	3323390
mode of action of nitro-heterocyclic compounds on escherichia coli.	the inhibitory and bactericidal activities of several different nitro-heterocyclic compounds, such as nitrofuran, nitronaphthofuran, nitrobenzofuran, nitroimidazole and nitrothiazole, were assessed in vitro. all these substances except nitroimidazole were active against escherichia coli, though to different degrees. under anaerobic test conditions the antibacterial activity increased slightly. nitroreductase-deficient mutants, however, were highly resistant to all nitro-compounds, indicating tha ...	1987	3322751
[medico-genetic studies of human provisional organs].		1987	3318529
anti-candida drugs--the biochemical basis for their activity.	the past years have seen a continuous effort toward the synthesis of new antifungal agents. most of them belong to the n-substituted imidazoles and triazoles. another interesting series of antifungals are the allylamines. biochemically, both the azole derivatives and the allylamines belong to the class of ergosterol biosynthesis inhibitors and thus differ from the polyene macrolide antibiotics. indeed, it is now believed that the antifungal action of the polyenes, nystatin and amphotericin b, is ...	1987	3319421
[experience with homemade substrates for mycology].	the meaning of the words "home-made", "medium" and "soil" is defined. the formulae are given and the manner for preparing lactritmel, lacmel and tritmel is described. the same for horse and cow dung agar soils is done. the lactritmel (20 fold diluted in water) is recommended for the specific diagnosis of candida albicans in its nascent culture. the first results are communicated, as obtained by slide culturing fungi on banana (musa paradisiaca) fruit pulp without previous sterilization. the amus ...	1987	3320629
production losses and control of helminths in ruminants of tropical regions.		1987	3294655
evaluation of the skin disinfecting activity and cumulative effect of chlorhexidine and triclosan handwash preparations on hands artificially contaminated with serratia marcescens.	the initial and cumulative efficacy of two antiseptic handwash preparations in eliminating serratia marcescens from hands was evaluated on volunteers. two antiseptics with persistent skin antibacterial activity, 4% chlorhexidine gluconate in detergent and 1.5% triclosan in natural soap, were studied in a new protocol designed according to food and drug administration guidelines. after a single handwash, both preparations exhibited a degerming action statistically superior to the mechanical elimi ...	1987	3294700
[field trial of an immunoenzyme method using beta-lactamase as the monoclonal antibody marker for the capsular antigen of the plague microbe].	beta-lactamase (penicillinase) has been used as a marker of monoclonal antibodies in the enzyme immunoassay made with a view to the detection of y. pestis capsular antigen and antibodies to it. the trial of the enzyme immunoassay with the use of the beta-lactamase conjugate in laboratories and under field conditions has revealed the advantage of this assay over hemagglutination tests commonly used for this purpose.	1987	3296577
purification and analysis of rat hematopoietic stem cells by flow cytometry.	the monoclonal antibody ox7 recognizes an epitope expressed on the thy-1 glycoprotein, ox22 recognizes the high molecular weight form(s) on leukocyte common antigen, and w3/13 recognized determinants found on certain sialoglycoproteins. recently, the rat colony-forming unit spleen (cfu-s) was characterized as being ox7 upper 20% positive (ox7u20%), ox22 negative (ox22-), and w3/13 weakly positive (w3/13+). in the present study these observations have been extended to include the hematopoietic st ...	1987	3297565
relevant problems to the epidemiology of mansonic schistosomiasis.		1987	3151110
[solid phase radioimmunoassay for the quantitative determination of antigen-specific igg-antibodies in the serum of patients with farmer's lung].	in this study we have investigated a number of technical aspects of a sensitive solid-phase radioimmunoassay (microtitre-plate ria) for the quantitative determination of antigen-specific igg antibodies in the sera of patients suffering from farmer's lung. microtitre-plates were coated with antigens of aspergillus fumigatus, micropolyspora faeni, aureobasidium pullulans, alternaria tenuis, thermoactinomyces vulgaris and hay. optimal antigen concentration and serum dilution were determined, the te ...	1987	3121779
[effect of bacterial toxins on microcirculation (experimental research)].	histological and biomicroscopic methods were used to study the effects of toxic lemic and meningococcal microbial fractions on mesenteric blood microcirculation in rats. aggregation ability of red blood cells and thrombocytes was investigated. the sublethal dose of meningococcal toxins was shown to induce marked impairment in the blood microcirculation 30 minutes after intravenous administration. the lethal dose of lemic toxins manifests itself later (in 2-4 hours). meningococcal toxins are char ...	1987	3105520
[transport of basic amino acids into yersinia pestis cells].	the transport of the basic amino-acids into the plague microbe cells has been shown to proceed by means of two transfer systems, the common transfer system and the system specific for lysine. besides that, arginine can be transported into the plague microbe cells by the histidine transport system. the vaccine strain ev niieg was used as a model to study the amino-acid transport systems.	1987	3106795
effects of neonatal estrogenization on rat bone development: a histomorphometric study.	the effects of a single dose of 500 micrograms of estradiol benzoate, administered on the first day of life, on rat bone development have been histomorphometrically studied at 15 days of age. estrogenized animals presented decreased total tibial length (16.55 +/- 0.50 vs. 17.84 +/- 0.73 mm, p less than 0.05) and increased thickness of the cartilage growth plate (528.92 +/- 13.30 vs. 382.77 +/- 37.85 micron, p less than 0.01). this increase was mostly due to the presence of a wider (p less than 0 ...	1987	3107771
reflex regulation of atrioventricular conduction.	we evaluated the time course of baroreflex modulation of atrioventricular (av) nodal conduction in anesthetized dogs (n = 28). beat-by-beat changes in heart rate (hr) and av interval (avi) evoked by transient alterations in arterial pressure (ap) were recorded in the intact state, after vagotomy, and following stellectomy. under each experimental condition, alterations in ap induced parallel changes in hr and avi with maximum hr and avi responses occurring simultaneously. in three animals, ap al ...	1987	3109256
effect of blood pressure on medial medulla-induced muscle atonia.	stimulation of the medial medullary reticular formation (mmrf) has long been reported to produce generalized inhibition of skeletal muscle activity. however, several studies have reported that in most cases mmrf stimulation produces only increases in muscle tone. in the present investigation we have found that blood pressure is a critical variable, determining whether mmrf stimulation will produce muscle excitation or inhibition. when mean arterial pressure (map) was greater than 80 mmhg but les ...	1987	3109262
the effect of sodium valproate on the subcellular particles of the rat liver.	sodium valproate, 200 mg/kg, i.p., for three weeks produced an increase in the mean sedimentation coefficient of liver mitochondria, while no changes were observed in lysosomes and peroxisomes. as the mean density of the mitochondria, the lysosomes and the peroxisomes of the control and treated animals were not different, it was concluded that the size of the mitochondria had increased in the liver of the treated rats. the mean sedimentation coefficient of the mitochondria returned to normal aft ...	1987	3109433
differences between saccharomyces cerevisiae and bacillus subtilis in secretion of human lysozyme.	saccharomyces cerevisiae secreted human lysozyme in the medium as an active form when the signal peptides of chicken lysozyme and a chicken lysozyme-aspergillus awamori glucoamylase hybrid were used, whereas it did not synthesize any human lysozyme protein by using the signal peptide of a. awamori glucoamylase. the secreted lysozyme was easily purified and crystallized. on the other hand, bacillus subtilis secreted an inactive human lysozyme, which seemed to have incorrect disulfide bonds, with ...	1987	3109419
differences between saccharomyces cerevisiae and bacillus subtilis in secretion of human lysozyme.	saccharomyces cerevisiae secreted human lysozyme in the medium as an active form when the signal peptides of chicken lysozyme and a chicken lysozyme-aspergillus awamori glucoamylase hybrid were used, whereas it did not synthesize any human lysozyme protein by using the signal peptide of a. awamori glucoamylase. the secreted lysozyme was easily purified and crystallized. on the other hand, bacillus subtilis secreted an inactive human lysozyme, which seemed to have incorrect disulfide bonds, with ...	1987	3109419
effects of inhibitors of the cytoplasmic structures and functions on the early phase of infection of cultured cells with simian virus 40.	to obtain information about cytoplasmic structures and functions involving the entry of simian virus 40 virions into cells, we examined whether the inhibitors that affect the functions and/or structure of lysosomes, cell membrane, and cytoskeletons inhibit expression of nuclear t antigen in the sv40-inoculated rat 3y1 and monkey cv-1 cells. chloroquine, methylamine, and butylamine did not inhibit t-antigen expression, suggesting that lysosomal acidification is not required for establishment of i ...	1987	3033894
plasmid-mediated virulence in yersiniae.		1987	3078833
detection of antigen to herpes simplex virus in cerebrospinal fluid from patients with herpes simplex encephalitis.	cerebrospinal fluid (csf) specimens were obtained from patients with presumed herpes simplex encephalitis who underwent brain biopsy for diagnostic confirmation. coded csf specimens were fixed on nitrocellulose filter paper and probed with a pool of monoclonal antibodies directed against four herpes simplex virus glycoproteins (gb, gc, gd, and ge). herpes simplex virus antigen was detected in 35 of 40 specimens obtained from 26 biopsy-positive patients. in contrast, only three of 25 specimens fr ...	1987	3033092
a factor discriminating between the wild-type and a mutant polyomavirus enhancer.	enhancers increase the frequency of transcription initiation from linked promoter elements, most probably as a result of the binding of specific proteins to the enhancer. the polyomavirus early region is expressed in differentiated mouse cells but not in undifferentiated embryonal carcinoma (ec) cells. this host range is a function of the enhancer because polyomavirus mutants selected for growth in ec cells have mutations in the enhancer and the host range is reproduced in transfection assays us ...	1987	3037380
cloning in escherichia coli of the gene specifying the dna-entry nuclease of bacillus subtilis.	with the aim of cloning genes involved in transformation of bacillus subtilis, a set of transformation-deficient mutants was isolated by means of insertional mutagenesis with plasmid phv60 (vosman et al., 1986). analysis of these mutants showed that those mapping in the aroi region lacked the dna-entry nuclease activity. plasmid phv60 derivatives, containing flanking chromosomal dna fragments, were isolated from these mutants and were used to screen a library of b. subtilis chromosomal dna in ph ...	1987	3038682
cloning in escherichia coli of the gene specifying the dna-entry nuclease of bacillus subtilis.	with the aim of cloning genes involved in transformation of bacillus subtilis, a set of transformation-deficient mutants was isolated by means of insertional mutagenesis with plasmid phv60 (vosman et al., 1986). analysis of these mutants showed that those mapping in the aroi region lacked the dna-entry nuclease activity. plasmid phv60 derivatives, containing flanking chromosomal dna fragments, were isolated from these mutants and were used to screen a library of b. subtilis chromosomal dna in ph ...	1987	3038682
fulminant leukemic polyradiculoneuropathy in a case of b-cell prolymphocytic leukemia. a clinicopathologic report.	a 52 year old man with a 10-month history of b-cell prolymphocytic leukemia (pll) died of an apparent acute fulminant polyradiculoneuropathy, a condition generally attributed to paraneoplastic complication. the pathologic examination disclosed diffuse leukemic infiltrations of the peripheral nervous system. it is suggested that this particularly aggressive form of b-cell chronic prolymphocytic leukemia presented a constellation of features that promoted the invasion of the peripheral nervous sys ...	1987	3040217
analysis of transfer genes and gene products within the trab-trac region of the escherichia coli fertility factor, f.	a series of plasmids that carry overlapping segments of f dna encoding the genes in the trab-trac interval was constructed, and a restriction enzyme map of the region was derived. plasmids carrying deletions that had been introduced at an hpai site within this interval were also isolated. the ability of these plasmids to complement transfer of f lac plasmids carrying mutations in trab, trav, and traw, and trac was analyzed. the protein products of the plasmids were labeled in uv-irradiated cells ...	1987	3040671
doctors against nuclear war in turkey.		1987	3120914
evaluation of the 11co2 positron emission tomographic method for measuring brain ph. i. ph changes measured in states of altered pco2.	the 11co2 method for measuring local brain ph with positron emission tomography (pet) has been experimentally evaluated, testing the adequacy of the kinetic model and the ability of the method to measure changes in brain ph. plasma and tissue time/activity curves measured during and following continuous inhalation of 11co2 were fit with a kinetic model that includes effects of tissue ph, blood flow, and fixation of co2 into compounds other than dissolved gas and bicarbonate ions. for each of ten ...	1987	3121647
[the structure of the variable gene for the kappa chains of antibodies produced by hybridoma ptf-02].	the genome of hybridoma ptf-02 has two genes for the kappa chains, and only one of these codes for the synthesis of the antibody light chains. the nucleotide sequences corresponding to the leader peptide and to the variable region of this gene were determined. an amino acid sequence corresponding to exons has been proposed on the basis of the nucleotide sequence. a nucleotide sequence adjacent to the gene at the 5'-end has also been determined, in particular, the precise localization of tata- an ...	1987	3116399
rescue of menotrophin cycles prone to develop ovarian hyperstimulation.	in an attempt to prevent the loss of 'overstimulated cycles' associated with human menopausal gonadotrophin (hmg)-induced ovulation, oestradiol levels and ovarian follicular state were monitored in 12 women with 'overstimulated cycles' after withholding hmg for several days. human chorionic gonadotrophin (hcg) was administered when oestradiol levels were less than or equal to 1700 pg/ml and the leading follicles between 17 and 22 mm in diameter. during the withholding period follicular growth co ...	1987	3122819
[studies on the antitumor effects of human interferon (2). effects of massive pulsatile administration of rifn-gamma on advanced renal cell carcinoma].		1987	3127620
a potential mechanism of dl-beta-hydroxybutyrate-induced malformations in mouse embryos.	dl-beta-hydroxybutyrate (dl-bohb) is teratogenic to rodent embryos in culture, but the biochemical mechanism(s) responsible for the induction of malformations has not been elucidated. thus, to delineate a potential mechanism, the interaction of the ketone body with embryonic glucose metabolism was investigated. mouse embryos were exposed in whole embryo culture to dl-bohb or each isomer separately during the period of neurulation. the results demonstrate that dl-bohb inhibits glucose oxidation b ...	1987	3111272
implication of ornithine decarboxylase and polyamines in pancreatic growth of neonatal rats.	the current study examines the effects of alpha-difluoromethylornithine (dfmo), an irreversible ornithine decarboxylase inhibitor, on pancreatic growth and development of rat neonates. newborn rats were given daily subcutaneous injections of 300 or 500 mg kg-1 dfmo and killed after 7, 14, 21, and 28 days of treatment. pancreatic weights and dna, rna, protein, amylase and chymotrypsin total contents (per pancreas) and concentrations were evaluated at the end of each period. inhibition of body wei ...	1987	3114741
parenteral antischistosomal therapy: a potential risk factor for hepatitis b infection.	to study the association between hepatitis b and schistosomiasis, 1,234 egyptian males, ages 18 to 24, were interviewed, examined, and tested for schistosoma mansoni infection and hbsag. sera from 91 (7.4%) of the study subjects were positive for hbsag, and s. mansoni was found in the stools of 26.3%. there was no correlation between s. mansoni infection, with or without hepatosplenomegaly, or a history of schistosomiasis, and hbsag. an association was found between hbsag positivity and a previo ...	1987	3119770
stereoselective fungal metabolism of 7,12-dimethylbenz[a]anthracene: identification and enantiomeric resolution of a k-region dihydrodiol.	syncephalastrum racemosum ut-70 and cunninghamella elegans atcc 36112 metabolized 7,12-dimethylbenz[a]anthracene (7,12-dmba) to hydroxymethyl metabolites as well as 7-hydroxymethyl-12-methylbenz[a]anthracene trans-3,4-, -5,6-, -8,9-, and -10,11-dihydrodiols. the 7,12-dmba metabolites were isolated by reversed-phase high-performance liquid chromatography and identified by their uv-visible absorption, mass, and nuclear magnetic resonance spectral characteristics. a comparison of the circular dichr ...	1987	3122656
[professional course for nurse instructors].		1987	260376
looking through the glass darkley.		1988	259286
through the eyes of continuing education. the icn.		1988	259614
sucking in the newborn infant during the first hour of life.		1988	259639
"essentially normal"--a vanishing phenomenon?		1988	259638
obstetric anesthesia and analgesia.		1988	2491117
[qualitative and quantitative changes in the phospholipid composition in experimental brain edema and during treatment with antiedema preparations].		1988	3174245
suppressive and enhancing effect of t-2 toxin on murine lymphocyte activation and interleukin 2 production.	t-2 toxin, a fungal metabolite shown previously to exert potent immunosuppressive effects, was examined for its effects on activation and interleukin 2 (il 2) production by murine and rat splenocytes. splenocytes (1 x 10(6) cells/well) were incubated with 1 microgram concanavalin a (con a) for 48 h at which time cellular protein and dna synthesis by these cells were ascertained using radiolabeled precursors. il 2 synthesis was determined from the cell supernatant using the il 2-requiring cell li ...	1988	3264300
malaria infection in rats with stimulated splenic red pulp: the blood flow and protective effect in normal and transplanted splenic tissue.	after splenic autotransplantation both weight and blood flow of the regenerated splenic tissue are decreased. in addition, the protective function of the transplant is less compared to that of the normal spleen. in the present study, the red pulp of normal and transplanted splenic tissue was stimulated by injections of phenylhydrazine to increase the weight, the blood flow, and the protective function. after stimulation, the weight of the normal spleen increased to 900 +/- 70 mg (control 530 +/- ...	1988	3265528
circular intermediates with missing nucleotides in the conversion of supercoiled or nicked circular to linear duplex dna catalyzed by two species of bal 31 nuclease.	the extracellular nucleases from alteromonas espejiana bal 31 can catalyze the endonucleolytic and/or exonucleolytic hydrolysis of duplex dna in response to a variety of alterations, either covalent or noncovalent, in dna structure. the nuclease can exist as at least two kinetically and molecularly distinct protein species. the two species that have been studied, called the 'fast' (f) and 'slow' (s) nucleases, both readily convert negatively supercoiled dnas to linear duplex molecules and accomp ...	1988	3275464
plasmodium fragile: detection of a ring-infected erythrocyte surface antigen (resa).	a ring-infected erythrocyte surface antigen (resa) has been detected by modified immunofluorescence assay in erythrocytes infected with the simian malaria parasite, plasmodium fragile. this resa, of mr 95,000, shares many characteristics with the resa initially found in the human malaria parasite p. falciparum. both antigens are found in the membrane of erythrocytes infected with young asexual parasite stages, in merozoite-enriched preparations, and in parasite culture supernatant. since the res ...	1988	3276549
the effects of some synthetic prostanoids on the contractility of the human lower uterine segment in vitro.	we have investigated the ability of three synthetic prostanoids to directly influence uterine contractility by studying the effects in vitro. strips of lower uterine segment smooth muscle were obtained from women undergoing elective cesarean section at term. the ability of these strips to develop tension in the presence of cumulative additions of prostanoids or oxytocin was assessed. spontaneous contractions were inhibited by zk 96.480, a stable synthetic analog of prostaglandin i2, with a 50th ...	1988	3277428
[continuous nasal positive pressure respiration (ncpap) as a therapeutic possibility in the sleep apnea syndrome].	sleep apnea syndrome (sas) is a rare disorder which is being diagnosed more often with increasing knowledge among physicians and patients. sas presents with daytime hypersomnolence, intellectual deterioration and personality changes, chiefly in obese men, and is caused by intermittent upper airway obstruction during sleep at the level of the mesopharynx. consecutive repetitive apneas of more than 10 seconds' duration are immediately abolished by pneumatic splinting with continuous positive press ...	1988	3278374
a comparison of culture, direct fluorescent antibody test, and a quantitative indirect immunoperoxidase assay for detection of chlamydia trachomatis in pregnant women.	an indirect immunoperoxidase assay that quantitates antichlamydial immunoglobulin g (igg) and iga antibodies in serum was compared with endocervical culture and direct fluorescent antibody (microtrak) for detection of chlamydia trachomatis in asymptomatic pregnant women. of the 64 women tested by the three methods, 22 (34%) had antichlamydial igg and iga in their serum. the culture was positive in nine patients (14%) and the microtrak was positive in eight (12.5%). all positive cultures were imm ...	1988	3279354
interleukin 1 as a tumor cytostatic mediator released from tumor ascites-treated macrophages.	peritoneal macrophages from dba/2 mice, elicited by injection of corynebacterium parvum (c.p.), were in vitro activated to eb tumor cytostasis by incubation with tumor-induced ascites that was harvested 7 days after intraperitoneal eb injection. the active cytostasis-mediating compound was found to be interleukin 1 (il 1). when tumor ascites was fractionated according to molecular weight size, the most active il 1-inducing fraction was found to comprise molecules of greater than 100,000 daltons. ...	1988	3264264
t-cell subsets in the cerebrospinal fluid and blood of patients with multiple sclerosis.	the absolute numbers and ratios of helper/inducer (t4) and cytotoxic/suppressor (t8) t-cells were determined in cerebrospinal fluid (csf) and blood of patients with multiple sclerosis (ms) and various other neurologic diseases (ond). in patients with ms, the t4:t8 ratio was higher in both blood and csf, and the increase was significantly greater in csf than in blood. these findings were due to an increased proportion of t4-lymphocytes in the csf and to a decreased proportion of t8-cells in blood ...	1988	3264287
antischistosomal treatment and the measurement of incidence of schistosomiasis in a community of high transmission.	there is disagreement in the measurement of incidence in schistosomiasis concerning which of two categories of negatives is representative of the population of an endemic area: naturally uninfected individuals (untreated negatives) and those made negative following treatment (treated negatives). the incidence of schistosomiasis was studied in the two categories of negatives for two periods among 208 school children (6-11 years old) living in a community of high transmission in zimbabwe. in both ...	1988	3147550
chromatographic studies of mitomycin c degradation in albumin microspheres.	serum albumins and polylactic acid (pla) have been used as bioerodable polymers in the preparation of drug-containing microspheres for parenteral drug delivery. the albumin microsphere may be prepared via either chemical cross-linking or heat denaturation of the protein. heat-denatured albumin microspheres containing mitomycin c (mmc) have been used in pre-clinical and clinical investigations. due to the high reactivity of mmc as a bifunctional alkylating agent, a study on the stability of mmc i ...	1988	3148622
characterization of the priming effects of human granulocyte-macrophage colony-stimulating factor on human neutrophil leukotriene synthesis.	human granulocyte-macrophage colony-stimulating factor (gm-csf) is an in vitro and in vivo stimulator of human bone marrow myelomonocytic precursor cells and mature granulocyte and macrophage effector cells. we have compared the effect of gm-csf on the synthesis of 5-lipoxygenase products induced by the chemotactic peptide fmet-leu-phe and the calcium ionophore a23187 in human neutrophils. although gm-csf alone did not stimulate detectable synthesis of products of the 5-lipoxygenase pathway, pre ...	1988	3148964
a role for glyceraldehyde-3-phosphate dehydrogenase in the development of thermotolerance in xenopus laevis embryos.	during heat shock, xenopus laevis embryos exhibit an increase in the rate of accumulation of lactate and a loss of atp relative to non-heat-shocked control embryos. these results suggest that heat shock stimulates a shift in energy metabolism to anaerobic glycolysis while at the same time causing an increase in the demand for atp. we have evidence indicating that the embryo may meet such demands placed on it by increasing the levels of some glycolytic enzymes. in this report, we show that heat s ...	1988	3182940
periovulatory serum prolactin and progesteron secretion in gonadotropin stimulated cycles.	in the group of 18 normoprolactinemic patients stimulated by human menopausal gonadotropins (hmg), serum prolactin (prl) and progesteron (p) levels were evaluated at the time of estradiol (e2) increase before, during and after chorionic gonadotropin (hcg) administration. in 8 patients (group i) mean serum prl levels remained normal, ranging from the mean basal values (12.4 +/- 1.7 micrograms/l) to the mean values at the time of hcg administration (16.8 +/- 1.8 micrograms/l) and after it (24.9 +/ ...	1988	3147564
[effect of the induction of liver cytochrome p-450 by a chemically inert fluorocarbon on the reactions of immediate and delayed hypersensitivity].	the effect of chemically inert perfluorodecalin (pfd) on the development of hypersensitive reactions of immediate and delayed types in guinea pigs and mice was studied. it was found that administration of pfd to animals both before sensitization (mice) and during the development of sensitization (guinea pigs) reduced the degree of the both types of hypersensitivity.	1988	3191983
effect of bromoethylamine hydrobromide on systemic acid-base balance.	intravenous administration of bromoethylamine hydrobromide (bea) has been shown to induce papillary necrosis of the kidney. we used this model to clarify the role of the medullary structure in acid-base homeostasis. bea (25 mg) or vehicle was injected to male sprague-dawley rats. blood specimens and 24 hr urine were collected once a week totaling 4 weeks. blood bicarbonate significantly decreased in bea treated rats with no change in plasma creatinine or creatinine clearance at 3 and 4 weeks, we ...	1988	3194903
treatment of fasciola hepatica infection in horses with triclabendazole.	thirteen thoroughbred foals with an adult infection and a presumed immature infection with fasciola hepatica were treated with 12 mg triclabendazole/kg bodyweight. the absence of eggs from samples of faeces examined at intervals up to 110 days after treatment showed that all the animals were cured. in a second trial the same dose of triclabendazole cured 11 of 12 foals whereas nitroxynil at 7 mg/kg cured only six of 12 foals. no side effects were observed after treatment.	1988	3195012
[lysosomal enzyme activity in the nerve tissue of various species of animals after administration of a neurotoxic pesticide aphos].		1988	3149613
production of prostaglandins and hete-compounds by isolated-cultivated bovine aortic endothelial cells.	the main product of aa conversion by baec is pgi2. other pgs were synthesized by confluent cells only in traces with exception of a hht-like compound which has still to be characterized in more details. a significant release of hete was only detected when baec were destroyed. under these conditions about 1 pmole/10(5) cells 15- and 12 hete were released by baec. taken into account the amounts of hete produced by endothelial cells as well as the partially contradictory activities of 12- and 15-he ...	1988	3150276
[levels of prostaglandin e1 and f2 alpha in the dynamics of toxic-infectious shock induced by yersinia pestis].	murine toxin of yersinia pestis when injected in the rat tail vein (ld50) caused pronounced alterations in pge1 and pgf2 alpha content in different tissues (lung, heart, spleen, liver, kidney, small intestine) and blood. heat-inactivated toxin has been shown to have the same effects as the intact toxin preparation. the changes in pg content are, probably, due to the lipopolysaccharide component of both preparations. the differences in metabolic effects between yersinia pestis endotoxin and lipop ...	1988	3162386
[bactericidal effect of tween 80 on mycobacteria].		1988	3210511
a double-blind comparison of ibuprofen, placebo and ibuprofen with meptazinol in soft tissue rheumatism.	the administration of ibuprofen potentiates and prolongs the analgesic effect of meptazinol when the two drugs are given simultaneously to mice. a double-blind three-way crossover study of placebo, ibuprofen (1600 mg/day) and ibuprofen (1600 mg/day) plus meptazinol (400 mg/day) was carried out in 45 patients with soft tissue rheumatism to see if the same potentiation could be demonstrated in man. treatment order was randomized and each regimen was given for 2 weeks preceded by 1 week on paraceta ...	1988	3163538
luteolytic potency of 16-phenoxy-derivatives of prostaglandin f2 alpha.	the binding of 16-phenoxy derivatives of prostaglandin (pg) f2 alpha to rat luteal membranes, and also their abortifacient potency in pregnant rats, have been studied. competitive binding studies with various pg-analogues were performed in ovaries of juvenile rats pretreated with pmsg and hcg, and in parallel studies the abortifacient potency of these substances was tested in pregnant rats. it was observed that this class of derivatives bound to the pgf2 alpha receptor as well as, or even better ...	1988	3162421
mechanisms of conversion of xanthine dehydrogenase to xanthine oxidase in ischemic rat liver and kidney.	previous studies have proposed and supported a role for the proteolytic, irreversible conversion of xanthine dehydrogenase to xanthine oxidase (xo) in postischemic injury in a wide variety of organs. a second mechanism of conversion, due to sulfhydryl modification and reversible with dithiothreitol (dtt), is potentially important but has not been well investigated. in this study rat liver and kidney were found to produce significant amounts of dtt-reversible xo during normothermic global ischemi ...	1988	3163235
jamshidi needle biopsy for diagnosis of bone lesions in small animals.	sixty-two bone lesions in 59 small animals were biopsied using a jamshidi-type biopsy needle. in all instances, the jamshidi needle biopsy diagnosis was confirmed or disputed by results of histologic examination of specimens obtained by surgical resection or by amputation or at necropsy. in 57 of 62 bone lesions, biopsy resulted in an accurate diagnosis of tumor vs nontumor, yielding an accuracy rate of 91.9%. the specific tumor type or lesion type was identified accurately by jamshidi needle bi ...	1988	3165373
[an immunocytochemical study on the prostaglandin e2 localization in human gastric mucosa].		1988	3165480
[effects of grf 1-29 in normal and hypotrophic lambs].	injections of hgrf rapidly stimulated gh secretion in lamb and this response was significantly higher in hypotrophic than in normal lambs. the treatment had an effect on body composition reducing the retention of lipids and the energy level and increasing calcium and phosphorus content. the results showed that grf affected both metabolism and skeletal growth of ruminants, stimulating specially the production of sm-c and proliferation of chondrocytes.	1988	3131856
receptor-like function of heparin in the binding and uptake of neutral lipids.	molecular mechanisms regulating the binding, amphipathic stabilization, and metabolism of the major neutral lipids (e.g., cholesteryl esters, triglycerides, and fatty acids) are well studied, but the details of their movement from a binding compartment to a metabolic compartment deserve further attention. since all neutral lipids must cross hydrophilic segments of plasma membranes during such movement, we postulate that a critical receptor-like site exists on the plasma membrane to mediate a ste ...	1988	3174644
production of tumor necrosis factor in nude mice by muramyl peptides associated with bacterial vaccines.	gram-negative vaccines can elicit the production of tumor necrosis factor (tnf) in mice primed by muramyl dipeptide (mdp) or by its lipophilic derivative mdp-dipalmitoyl glycerol (mdp-gdp). in mice pretreated with mdp and particularly with mdp-gdp, bordetella pertussis vaccine was shown to be more effective than typhoid vaccine. the time course of tnf production in the blood did not indicate any difference between the effect of mdp or of mdp-gdp. in both cases the cytotoxic activity reached maxi ...	1988	3167834
[effect of the entomopathogenic fungus penicillium funiculosum on the reproduction of the causative agent of plague in the body of fleas].		1988	3173243
examining the impact of different health levels and management strategies on dairy heifer production through computer simulation.		1988	3232667
magnesium salts exert direct vasodilator effects on rat cremaster muscle microcirculation.	in vivo microscopic observations were made on arterioles and venules (10-20 microns i.d.) of the rat cremaster muscle circulation, using an image-splitting eyepiece and high-resolution tv image-intensification, in order to determine the microvascular effects of inorganic and organic magnesium (mg) salts. mgso4, mgcl2, mg aspartate hcl, and mg acetate were administered perivascularly and intravenously. topical application (10(-2) to 10(1) mumol) and intravenous (i.v.) administration (5-400 mumol/ ...	1988	3233048
funding of project to map human genes sparks debate.		1988	3177203