Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [effect of sulfonamides on the fatty acid composition of enterobacterial lipids]. | 1985 | 3917183 | |
| inefficacy of penicillin v in acute laryngitis in adults. evaluation from results of double-blind study. | patients with acute laryngitis following an upper respiratory tract infection are often treated with antibiotics for their voice complaints, although, to our knowledge, the effect of such therapy has not been examined. in the present study, comprising 100 adults with laryngitis, the rate of resolution of vocal symptoms, as estimated from voice recordings or subjectively by the patients, was the same in patients who received penicillin v (pcv) as in those who received placebo. similarly, the degr ... | 1985 | 3918495 |
| in vivo efficacy and ultrastructural effects of mitomycin c against experimental alveolar hydatid disease. | the in vivo efficacy and ultrastructural effects of mitomycin c were determined against alveolar hydatid disease in experimentally infected animals and compared to mebendazole treatment. mitomycin c inhibited the mean cyst mass of treated versus control animals by 84.1% which was statistically significant at the alpha = 0.01 level. mebendazole given daily inhibited the mean cyst mass by 80.1%, while mebendazole administration on the same treatment schedule as that used for mitomycin c inhibited ... | 1985 | 3921601 |
| effect of human gamma interferon on invasiveness of salmonella typhimurium in hep-2 cell cultures. | the effect of human gamma interferon (huifn-gamma) on the invasiveness of salmonella typhimurium, shigella flexneri and yersinia enterocolitica serotype 0:3 in hep-2 cell cultures was examined. the intracellular and extracellular bacteria were identified by a combination of nomarski differential interference contrast microscopy and uv incident light microscopy applied on the same microscope. pretreatment of hep-2 cells with huifn-gamma reduced in a dose dependent manner the number of s. typhimur ... | 1985 | 3921632 |
| properties of human anti-lipopolysaccharide gamma globulin: specificity and protective effects. | blood donated to the natal blood transfusion service was screened by an enzyme-linked immunosorbent assay (elisa) for anti-lipopolysaccharide (anti-lps) antibodies. plasma units with high concentrations (greater than 40 micrograms/ml) of anti-lps igg were pooled and fractionated to obtain a gamma globulin (lot lg-1). the binding of lg-1 antibodies to lps prepared from 14 bacterial species and strains was found to be the highest to lps from shigella flexneri, salmonella abortus equi and salmonell ... | 1985 | 3922117 |
| isolation and characterization of a new endo-beta-galactosidase from diplococcus pneumoniae. | an endo-beta-galactosidase, which hydrolyzes the internal beta-galactosidic linkages of r----glcnac (or galnac) beta 1----3gal beta 1----4glcnac (or glc), was isolated from the culture supernatant of diplococcus pneumoniae. the enzyme, named endo-beta-galactosidase dii, hydrolyzed linear n-acetyllactosamine repeating structures in glycolipids and glycopeptides to release oligosaccharides. the specificity of endo-beta-galactosidase dii is the same as that of escherichia freundii endo-beta-galacto ... | 1985 | 3922410 |
| pressure- and temperature-dependent adhesion of pseudomonas aeruginosa to hep-2 cells. | pseudomonas aeruginosa bacteria adhere to human epitheloid (hep-2) cells in culture. under normal atmospheric conditions (1 ata), this adhesion increased significantly when the temperature rose from 22 to 37 degrees c. under hyperbaric atmosphere (= air, 7 ata) conditions, a similar, significant enhancement of bacterial adhesion to the cells was noted when the temperature rose. if the temperature was kept stable at 22 or 37 degrees c and the pressure was increased from 1 to 7 ata, a pressure-ind ... | 1985 | 3925713 |
| radioimmunoassay of thyroxine in saliva. | a simple radioimmunoassay (ria) for thyroxine (t4) in saliva has been described. fifty euthyroid control subjects, 14 euthyroid pregnant women, 23 thyreotoxic and 10 hypothyroid patients were examined. serum t3, t4, thyroxine binding globulin (tbg) and tsh were measured simultaneously. the mean level of t4 in saliva in controls was 1.10 +/- 0.07 nmol/l. there was a good correlation between the saliva and serum t4 concentrations (r = 0.74) and between saliva t4 values and the t4/tbg ratio (r = 0. ... | 1985 | 3926523 |
| fractionation of radioactivity in the milk of goats administered 14c-aflatoxin b1. | a detailed fractionation of radioactivity in the milk of goats administered 14c-aflatoxin b1 at low doses was performed. the milk collected in the first 24 h following dosing contained radioactivity equivalent to 0.45-1.1% of the dose given. the radioactivity in each sample was partitioned into 4 fractions: ether, protein, dichloromethane, and water-alcohol. over 80% of the radioactivity was detected in the dichloromethane fraction, of which over 95% was attributable to aflatoxin m1. no aflatoxi ... | 1985 | 3926742 |
| fractionation of radioactivity in the milk of goats administered 14c-aflatoxin b1. | a detailed fractionation of radioactivity in the milk of goats administered 14c-aflatoxin b1 at low doses was performed. the milk collected in the first 24 h following dosing contained radioactivity equivalent to 0.45-1.1% of the dose given. the radioactivity in each sample was partitioned into 4 fractions: ether, protein, dichloromethane, and water-alcohol. over 80% of the radioactivity was detected in the dichloromethane fraction, of which over 95% was attributable to aflatoxin m1. no aflatoxi ... | 1985 | 3926742 |
| improved control in advanced head and neck cancer with simultaneous radiation and cisplatin/5-fu chemotherapy. | chemotherapy with cisplatin plus 5-fu infusion was given simultaneously with radiation therapy; both were administered every other week. twenty-seven patients with stage iv disease and three with stage iii disease were treated; 57% had t4, 33% had n3, and 10% had m1 disease. of these patients, 17 (57%) achieved complete response and the rest achieved partial response. however, whether or not a clinically complete response occurred had no effect on subsequent risk for disease recurrence. with a m ... | 1985 | 3928158 |
| in vitro pyocin activity of pseudomonas aeruginosa strains pretreated with antibiotics. | fifty-six selected strains of pseudomonas aeruginosa belonging to 8 different pyocin types (h, i, 15, 6, pti-1, pti-2, pti-3, pti-4) were treated with subinhibitory concentrations (mic/2) of either gentamicin or carbenicillin. both treatments induced changes in pyocin patterns for all types but at different levels. the percentage of strains that retained their pyocin pattern were more or less equal in both treatments. in treated and untreated producers, the growth inhibition ability for 5 differ ... | 1985 | 3928466 |
| clues to the puzzle of purpose. | 1985 | 3929138 | |
| [study on an intestinal loop test of virulent shigella bacillus]. | 1985 | 3932548 | |
| selected overview of nongynecologic surgical intra-abdominal infections. prophylaxis and therapy. | true prophylaxis of intra-abdominal nongynecologic infections is limited to elective, nonemergency surgery and is best shown in three clean-contaminated surgical procedures. all of these have an infection rate of approximately 10 to 20 percent and include all colon resection surgery, most gastric surgery, and about one third of the cholecystectomies for chronic calculous cholecystitis. each of these three surgical procedures has a somewhat different pattern of bacterial pathogens. the most usefu ... | 1985 | 3934966 |
| [enhanced effectiveness of antibiotic therapy with an exogenous lysozyme in dysentery in children]. | the course of dysentery and the host immune responsiveness were studied in 171 children with acute flexner's and sonne dysentery. 67 children were treated with monomycin in combination with lysozyme and 104 children with monomycin alone. it was shown that the recovery period in children treated with monomycin and lysozyme was shorter as compared to that in children treated with monomycin alone. the immunity characteristics in children treated with monomycin were closer to the physiological value ... | 1985 | 3936408 |
| evaluation of chemotherapy with benznidazole and nifurtimox in mice infected with trypanosoma cruzi strains of different types. | a test was made of the susceptibility of 30 strains of trypanosoma cruzi to chemotherapy with nifurtimox (bay 2502) and benznidazole (ro 7-1051). the strains had previously been classified as type i, ii, or iii according to their morphobiological and isoenzymic characteristics. three type i strains, 14 type ii strains, and 13 type iii strains were studied. mice were infected with 2 x 10(5) blood forms of these parasites and treated for 90 days with benznidazole or nifurtimox. all the surviving m ... | 1985 | 3936634 |
| chlamydial proctitis? | chlamydia trachomatis was isolated from 21 (7%) of 309 specimens obtained in october 1982 from the rectum of homosexual men undergoing proctoscopy. during the same period neisseria gonorrhoeae was isolated from 12 (3%) of 454 specimens obtained similarly. the clinical features of patients infected with each of these micro-organisms were compared with those of an uninfected group of homosexual men. no characteristic diagnostic features were noted in the infected men. | 1985 | 3936777 |
| arteriovenous flow reversal, experimental investigations. i. complete flow reversal of the small intestine and of the rear extremity of the dog. | segments of the small intestine and hind extremities of dogs were perfused under the condition of complete arterio-venous flow reversal (avfr). in the intestinal segments, the peripheral resistance under avfr conditions was 4.4 times higher than under orthograde perfusion. petechiae and hemorhagic infarction were observed. the average flow resistance of the hind extremities was 2.4 times higher than the resistance in control extremities. the po2 and the o2 saturation in the blood, outflowing fro ... | 1985 | 3937361 |
| assessment by a two-site enzyme immunoassay of human epidermal growth factor (urogastrone) in the urine of patients with various gastrointestinal diseases including malignant tumors. | by using our two-site enzyme immunoassay (eia) system, the levels of human epidermal growth factor (hegf) in the urine of patients with various gastrointestinal diseases including malignant tumors were measured. urinary excretion of hegf in patients having undergone gastric resection, expressed as a function of creatinine, was found to be somewhat decreased. while the levels of hegf in patients with gastric cancer were significantly increased. then, the molecular features of hegf in the urine of ... | 1985 | 3937528 |
| initiation and promotion in young and old animals. implications for human tumour formation. | the significance of ageing in experimental skin carcinogenesis and on the manifestation of tumour formation in man was studied. initiation with the carcinogenic hydrocarbon 7,12-dimethylbenz [a]anthracene and promotion with croton oil in mouse skin showed that tumour formation was unchanged when promotion was delayed 3, 10, 23 or 43 weeks in comparison with that in untreated animals of the same age. the sensitivity to carcinogen treatment of animals over 71 weeks of age was less than that of ani ... | 1985 | 3938448 |
| [virulence of shigella: epithelial cell invasiveness and other dysentery-producing factors]. | 1985 | 3938489 | |
| [theoretical conformation analysis of tetrasaccharide-repeated links of the shigella flexneri o-antigenic polysaccharide]. | theoretical conformational analysis of four tetrasaccharide repeating units of the shigella flexneri serogroup y polysaccharide has been carried out. interdependency of conformational states of neighbouring disaccharide units in the oligosaccharides has been investigated and conformational distribution of tetrasaccharides in solution calculated. taking into account the entropy of oligosaccharide chains is shown to lead to significant correction of the results. | 1985 | 2408628 |
| a heat-modifiable outer membrane protein carries an antigen specific for the species yersinia enterocolitica and yersinia pseudotuberculosis. | the outer membranes of gram-negative bacteria are considered to be of importance in host-bacteria interaction, in protective immunity, and occasionally in subclassification within a species. in this study, the outer membranes of several strains of yersinia enterocolitica and y. pseudotuberculosis were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). it was found that the appearance of the major proteins depended on the temperature at which they were solubilized i ... | 1985 | 2409152 |
| [synthesis of oligosaccharide fragments of o-specific polysaccharides of shigella flexneri. iii. synthesis of the tetrasaccharide glc alpha 1-3rha alpha 1-2(glc alpha 1-3)rha alpha 1-ome and the pentasaccharide glcnac beta 1-2(glc alpha 1-3)rha alpha 1-2(glc alpha 1-3)rha alpha 1-ome]. | two synthetic schemes to prepare the title branched tetrasaccharide and pentasaccharide are described. these oligosaccharides represent fragments of the o-antigenic polysaccharides of shigella flexneri serotype 5b. | 1985 | 2412567 |
| autoaggressive t lymphocyte lines recognizing the encephalitogenic region of myelin basic protein: in vitro selection from unprimed rat t lymphocyte populations. | autoimmune t lymphocyte lines have been established from unprimed normal rat lymph node cell populations. in a first, negative-selection round, spontaneously proliferating (smlr) t cells were eliminated by a pulse of budr followed by short wave light irradiation. in a second, positive-selection round, the smlr-depleted populations were confronted with mbp presented by syngeneic spleen adherent cells. reactive t cells were propagated until stable, permanent t lines were established. all lines wer ... | 1985 | 2413113 |
| [membrane activity of a cell-free filtrate of shigella flexneri 2a]. | in the course of metabolism in shigella flexneri 2a strain there was noted secretion of a membrane-tropic factor into media. this factor increased the conductivity of bilayer lipid membranes causing formation of ionic channels in them. minimal conductivity of the channels was about 25 ps in 0.5 m nacl; at ph 7-8 the channels exhibited cation-anion selectivity. the calculated number of cation transfer (t+) was elevated with an increase in the channel amplitude, approaching 0.86. since the conduct ... | 1985 | 2413620 |
| [use of immunoenzyme analysis for evaluating the specificity of the antibody recognition of the antigenic determinants of streptococcus group a]. | investigations carried out with the use of a celia system have revealed that antibodies in the sera of patients with primary erysipelas and antibodies in rabbit antiserum to the ribosomes of group a streptococcus specifically bind with adsorbed streptococcal ribosomes, recognizing the antigenic determinants of streptococcal ribosomes, which differ from those of individual gram-negative prokaryotes (escherichia coli, shigella sonnei, shigella flexneri, salmonella minnesota). the modified celia sy ... | 1985 | 2413662 |
| [immune response and immune tolerance to the o-antigen of shigella flexneri vi]. | the method for the determination of the number of cells synthetizing antibodies to s. flexneri vi o-antigen in the spleen of mice has been developed. primary immune response to this antigen has been studied with the use of the new method. immune response to the optimum immunogenic dose of o-antigen has a manifest variable character. the intensity of primary immune response has been shown to rise with the increase of the dose of o-antigen from 0.004 to 50 micrograms. the preliminary injection of ... | 1985 | 2413664 |
| genetic determinants of virulence in shigella and dysenteric strains of escherichia coli: their involvement in the pathogenesis of dysentery. | 1985 | 2414072 | |
| [specific antigens of the causative agents and their antibodies in the circulating immune complexes in acute dysentery]. | the level of circulating immune complexes has been determined in 53 patients in the dynamics of the disease. for the first time circulating immune complexes have been found to contain shigella sonnei k-antigen and shigella flexneri o-antigen, as well as iga, igg and igm to shigella. shigella antigens can be detected from the first week of the disease, and their occurrence does not depend on the level of circulating complexes in patients blood serum. | 1985 | 2414952 |
| [effect of vitamins of the b group on the accumulation of complex antigens of dysentery bacteria in the process of deep cultivation]. | 1985 | 2416151 | |
| [comparative characteristics of antigenic conversion induced by shigella flexneri and escherichia coli 0129 phages]. | the interaction of s. flexneri converting phages pe5, p90 and fv with e. coli antigenic variant o129, e. coli o129 converting phage vb with the above antigenic variant and with s. flexneri y-variant was studied. phage pe5 and phage vb were found to induce the conversion of o-antigen in e. coli antigenic variant 0129 and in s. flexneri y-variant with the detection of antigens v and 7,8. phages p90 and fv induced no conversion of o-antigen. changes in the antigenic properties of convertants were c ... | 1985 | 2416154 |
| trials with portable screen rooms modified for use as animal-baited net traps for mosquito collection. | trials in larimer county, colorado during july and august 1984, with recreational screen rooms modified as large animal-baited mosquito traps are described. the two units tested are free-standing, portable and require no external support. in all-night trials, 462.5 mosquitoes/trap night were captured with horse bait compared with 367/trap night with cdc light traps. in 2-hour evening comparisons, mosquitoes collected per trapping period totalled 416 for horse-bait traps, 132 for light traps, and ... | 1985 | 2906667 |
| striatal synaptosomal dopamine synthesis: evidence against direct regulation by an autoreceptor mechanism. | regulation of the rate-limiting step in dopamine (da) synthesis was estimated in striatal synaptosomes by measuring the rate of hydroxylation of l-4-[3h]phenylalanine, a substrate of tyrosine hydroxylase (th). da inhibited hydroxylation with an ic50 of 0.2 microm. the concentration-response curve of da-induced inhibition was not affected by the presence of 1 microm chlorpromazine, a phenothiazine da antagonist. sulpiride and haloperidol, da antagonists of the benzamide and butyrophenone classes ... | 1985 | 2859213 |
| prolactin and its involvement in fluid regulation in the bovine. | plasma prolactin (prl) concentrations, osmolality, water consumption, feed intake, urine excretion, and fecal water output were determined in twelve steers of 3 breeds exposed to 5 feed and water regimes. breed differences were found in water intake and plasma prl concentrations when feed and water were ad lib., however, during any of the other 4 treatments, responses were similar between breeds. during dehydration and feed restriction, water intake, urine, fecal water, and plasma prolactin decr ... | 1985 | 2859961 |
| effects of excitatory amino acids on calcium transport by brain membranes. | the effect of excitatory amino acids on the uptake of 45ca was studied in crude mitochondrial (p2) fractions prepared from mouse brain. l-glutamate stimulated calcium uptake, but this action was not shared by other amino acids including d-glutamate, l-aspartate, n-methyl-aspartate or kainate. the glutamate-stimulated calcium uptake was, however, blocked by inhibitors of glutamate dehydrogenase, such as d-glutamate, glutarate and triiodothyronine. subcellular fractionation demonstrated that the u ... | 1985 | 2860953 |
| chemotaxis by entamoeba histolytica. | a micropore membrane procedure to assay taxis by entamoeba histolytica is described and the results of studies of responses to a variety of soluble substances, bacteria, an rat colon washings using this procedure are reported. trophozoites migrated in blind well chambers through 8-micron pore size polycarbonate membranes but not nitrocellulose membranes up to 12 micron pore size. amoebae were attracted toward fresh axenic culture medium (tyi-s), an enzymatic hydrolysate of casein (trypticase), a ... | 1985 | 2861283 |
| antibodies to htlv-i p24 in african and portuguese populations. | using a radioimmunoassay to detect htlv-i protein antibodies of molecular weight 24,000, we screened populations from algeria (140 subjects), tunisia (442), mali (69), senegal (415), uganda (135), the central african republic (77), the congo (360), and madagascar (193). only four subjects were positive (1 from senegal, 1 from uganda, 2 from the congo). this is a much lower figure than that found by others in africa by the enzyme-linked immunosorbent assay technique. in addition, 319 portuguese b ... | 1985 | 2861895 |
| transformation of primary cultured and co-cultured adult rat liver cells by 3'-methyl-4-dimethylaminoazobenzene. | under various conditions of culture and carcinogen treatment, the transformation of liver cells by 3'-methyl-4-dimethylaminoazobenzene (3'-me-dab) was studied. primary liver cell (plc) cultures from adult male rats and co-cultures with plcs of arl-d8 cells of a liver epithelial-like clear cell line from adult female rats were treated with 0.24 mm 3'-me-dab for 6 days. four of 8 carcinogen-treated plc cultures contained cells with marker chromosomes, and 3 of the 8 cultures contained gamma-glutam ... | 1985 | 2862759 |
| [relationship between hosts and hemorrhagic fever renal syndrome in chang-an county, shan-xi province]. | 1985 | 2862998 | |
| characterization of enhanced intestinal permeability; electrophysiological study on the effects of diclofenac and ethylenediaminetetraacetic acid. | the mechanism of the enhancing effects of diclofenac sodium and disodium ethylenediaminetetraacetate (edta) on intestinal permeability to sulphanilic acid were investigated in the rat isolated jejunum by an electrophysiological technique. the results suggested that diclofenac sodium increased the mucosal-to-serosal flux rate of sulphanilic acid by enhancing both transcellular and paracellular permeabilities while the effect of edta was located only in the paracellular pathway. | 1985 | 2863363 |
| iga response during accidental infection with shigella flexneri. | 1985 | 2863666 | |
| combination of mefloquine with sulfadoxine-pyrimethamine compared with two sulfadoxine-pyrimethamine combinations in malaria chemoprophylaxis. | 1985 | 2863679 | |
| immunocytochemical studies of somatostatin neurons in brain. | immunohistochemical studies with antisera to somatostatin have, in many instances, led the way to our present understanding of the peptidergic nervous system. somatostatin was among the first of the hypophysiotropic hormones shown to be contained in diverse neuronal circuits outside of the hypothalamus. for example, somatostatin is found within neurons ranging in location from the cerebral cortex to primary sensory neurons to enteric neurons within the gut wall. somatostatin was also the first n ... | 1985 | 2863929 |
| measurement of igg in equine blood by immunoturbidimetry and latex agglutination. | this paper describes the quantitative measurement of igg concentrations in equine blood/serum by turbidimetric analysis and the qualitative assessment using latex agglutination. the concentration of igg in foal serum measured by turbidimetry correlated well with the results obtained by radial immunodiffusion (r = 0.91) and the gamma-globulins estimated from the electrophoresis of serum on cellulose acetate (r = 0.92). the method, using antibody-coated latex beads, to detect foals with serum igg ... | 1985 | 2580703 |
| structural analysis of the o-glycosidically linked core-region oligosaccharides of human meconium glycoproteins which express oncofoetal antigens. | glycoproteins were extracted from meconium samples of group o neonates of secretor type by pronase digestion followed by precipitation in 67% aqueous ethanol and separated into ii antigen enriched and depleted fractions by affinity chromatography. the latter fraction strongly expressed the oncofoetal antigens recognised by natural antibodies in mouse sera and the hybridoma antibody fc 10.2, and this activity was enhanced after mild acid hydrolysis to remove sialic acid and fucose residues. oligo ... | 1985 | 2580706 |
| the effects of subdiabetogenic streptozotocin doses on rat beta cell volume and functions. | the influence of a single i.v. injection of 30 mg/kg streptozotocin (sz) into female wistar rats on plasma glucose, pancreatic insulin content, volume and function of the remaining beta-cells has been investigated. 14 days after sz treatment most animals remained normoglycemic; only about 15% showed permanent hyperglycemia. the pancreatic insulin content in normoglycemic rats was reduced to 30%, whereas in hyperglycemic rats only 6% of control values could be detected. the remaining beta-cell vo ... | 1985 | 2581562 |
| [synthesis of oligosaccharide fragments of o-specific shigella flexneri polysaccharides. ii. synthesis of trisaccharide glc alpha1----3rha alpha1----2rha alpha1----ome and tetrasaccharide glcnac beta1----2(llc alpha1----3)rha alpha1----2rh alpha1----ome]. | methyl glycosides of the title linear trisaccharide and branched tetrasaccharide were synthesized by stepwise glycosylation. these oligosaccharides represent the fragments of o-antigenic polysaccharides of shigella flexneri serotypes 2b, 3a, 5b, and x. | 1985 | 2581587 |
| inflammatory bowel disease. | the place of inflammatory bowel disorders in the spondarthritis matrix has been discussed and aetiopathological and clinicoradiological features of their individual arthropathies described. particular emphasis has been placed on the arthropathies of ulcerative colitis and crohn's disease, but a comment is also included on the much rarer condition, whipple's disease. the position of reactive arthritis resulting from enteric infection in relation to the spondarthritis concept is examined in the li ... | 1985 | 2581732 |
| human t-cell leukemia/lymphoma viruses: atl and aids. | 1985 | 2897327 | |
| release of atrial natriuretic peptide by atrial distension. | a heterologous radioimmunoassay was used to measure the concentration of immunoreactive atrial natriuretic peptide (ianp) in plasma from the femoral artery of eight chloralose anaesthetized dogs. mitral obstruction which increased left atrial pressure by 11 cmh2o increased plasma ianp from 97 +/- 10.3 (mean +/- se) to 135 +/- 14.3 pg/ml. pulmonary vein distension increased heart rate but did not increase plasma ianp. bilateral cervical vagotomy and administration of atenolol (2 mg/kg) did not pr ... | 1985 | 2931169 |
| thyroxine induced transformation in sarcoplasmic reticulum of rabbit soleus and psoas muscles. | the properties of the sarcoplasmic reticulum membranes isolated from slow-twitch type i soleus and fast-twitch type ii psoas muscles of control and thyroxine treated rabbits were comparatively studied. membrane yield, maximal calcium storing capacity, atp-supported calcium uptake, calcium-dependent atpase activity and calcium-dependent phosphoprotein formation were found to be 3-10 fold higher in psoas than in soleus preparations. membrane yield, calcium-dependent atpase activity, atp-supported ... | 1985 | 2934902 |
| cellular and vessel wall morphology of cerebral cortical arterioles after short-term diabetes in adult rats. | microvascular pathology is one of the major problems associated with prolonged diabetes mellitus. the purpose of the present study was to determine if anatomical cellular pathology of the cerebral cortical microvessels could be detected very early after the onset of a streptozotocin-induced model of diabetes. male rats at age 14-16 weeks were hyperglycemic (greater than 250 mg/dl) for 4 weeks prior to scanning and transmission electron microscopic analysis of the external and internal cell and v ... | 1985 | 2935212 |
| [effects of cycleanine dimethobromide on heart conduction system]. | 1985 | 2945367 | |
| characterization of a major protein with a molecular weight of 160,000 associated with the viral capsid of epstein-barr virus. | a monoclonal antibody designated v3 was produced against a late protein associated with the epstein-barr virus-induced viral capsid antigen complex. the antibody reacted with discrete patches in the nuclei of infected cells as well as with virus particles, as shown by immunofluorescence and ultrastructural immunoperoxidase staining. the molecular weight of the protein precipitated by this monoclonal antibody was ca. 160,000. all anti-viral capsid antigen antibody-positive sera tested in an enzym ... | 1985 | 2981328 |
| middle tumor antigen of polyomavirus transformation-defective mutant ng59 is associated with pp60c-src. | we have found that lysis of mouse embryo cells infected with the polyomavirus host range transformation-defective (hr-t) mutant ng59 under gentle conditions that avoid ionic detergents results in detectable ng59-encoded middle tumor antigen (mtag) associated with pp60c-src. this mtag-pp60c-src complex could be immunoprecipitated from ng59-infected cell lysates by either sera from animals bearing polyomavirus-induced tumors or by monoclonal antibodies directed against mtag. immune complex kinase ... | 1985 | 2981329 |
| genome organization of herpesvirus aotus type 2. | herpesvirus aotus type 2, a virus commonly found in owl monkeys without overt disease, has a similar genome structure to the oncogenic herpesviruses of nonhuman primates (herpesvirus saimiri, herpesvirus ateles). virion dna of herpesvirus aotus type 2 (m-dna) has an unique 110-kilobase-pair region of low g + c content (40.2%, l-dna), inserted between stretches of repetitive h-dna (68.7% g + c, about 41 kilobase pairs per molecule) that are variable in length. a minority of virions contain defect ... | 1985 | 2981331 |
| autoregulation of the bacteriophage p22 scaffolding protein gene. | during the formation of each bacteriophage p22 head, about 250 molecules of the product of gene 8, scaffolding protein, coassemble with and dictate correct assembly of the coat protein into a proper shell structure. at approximately the time that dna is inserted inside the coat protein shell, all of the scaffolding protein molecules leave the structure. they remain active and participate in several subsequent rounds of shell assembly. previous work has shown that scaffolding protein gene express ... | 1985 | 2981337 |
| analysis of mobilization elements in plasmids from shigella flexneri. | the mobilization properties of three plasmids were examined after cotransfer from shigella flexneri to escherichia coli. the largest plasmid, pcn1, was shown to be a conjugative r factor that could promote its own transfer and allow cotransfer of a 4.1-kilobase plasmid, pcn3; mobilization of the third plasmid, pcn2 (6.3 kilobases), required the presence of both pcn1 and pcn3. sequences from pcn2 and pcn3 homologous to the bom (basis of mobilization) sites of cole1 and pbr322 were localized by an ... | 1985 | 2982788 |
| adenine nucleotides directly stimulate pertussis toxin. | both cholera toxin and pertussis toxin catalyzed adp-ribosylation of purified bovine brain tubulin. the effect of cholera toxin was evident in the absence or presence of nucleotides. in contrast, pertussis toxin required adenine nucleotides for its adp-ribosylating activity. atp, atp gamma s, app(nh)p, deoxy-atp, and adp all supported pertussis toxin-catalyzed adp-ribosylations in the absence or presence of edta, suggesting that nucleotide hydrolysis was not involved. adenine nucleotides also pr ... | 1985 | 2982826 |
| a new beta-lactamase plasmid in neisseria gonorrhoeae. | 1985 | 2984155 | |
| escherichia coli 23s ribosomal rna truncated at its 5' terminus. | in a strain of e. coli deficient in rnase iii (abl1), 23s rrna has been shown to be present in incompletely processed form with extra nucleotides at both the 5' and 3' ends (king et al., 1984, proc. natl. acad. sci. u.s. 81, 185-188). rna molecules with four different termini at the 5' end are observed in vivo, and are all found in polysomes. the shortest of these ("c3") is four nucleotides shorter than the accepted mature terminus. in growing cells of both wild-type and mutant strains up to 10% ... | 1985 | 2987818 |
| opioid modulation of ingestive behaviors in woodchucks and racoons. | we have examined the effect on feeding of opioid blockade with naloxone in two species which demonstrate a marked seasonality in their feeding patterns, the racoon (procyon lotor) and the woodchuck (marmota monax). naloxone suppressed food intake in the woodchuck which is a true hibernator. naloxone failed to suppress food intake in the racoon and, in fact, enhanced intake of a preferred sucrose solution. in the racoon, ir-dynorphine concentrations were extremely high in the hypothalamus compare ... | 1985 | 2987986 |
| isolation of retinoic acid-resistant clones from human breast cancer cell line mcf-7 with altered activity of cellular retinoic acid-binding protein. | after ethyl methane sulfonate mutagenesis of the mammary carcinoma cell line, mcf-7, we have isolated three clones, u-2, u-3, and u-9, resistant to retinoic acid. these three clones showed more than a 1000-fold higher level of resistance to retinoic acid than the parental mcf-7 cells when assayed by colony formation in monolayer culture system or by growth curves. the three resistant clones showed a 200-fold higher resistance to 13-cis-retinoic acid, about 10-fold higher resistance to retinol, a ... | 1985 | 2988768 |
| mercuric reductase structural genes from plasmid r100 and transposon tn501: functional domains of the enzyme. | the nucleotide sequence for the 2240 bp of plasmid r100 following the merc gene of the mercuric resistance operon has been determined and compared with the homologous sequence of transposon tn501. the sequences following merc and preceding the next structural gene mera are unrelated between r100 and tn501 and differ in length, with 72 bp in tn501 and 509 bp in r100. the r100 sequence has a potential open reading frame (orf) for a 140 amino acid polypeptide with a reasonable translational start s ... | 1985 | 2989109 |
| cloning of plasmid dna sequences involved in invasion of hela cells by shigella flexneri. | a large plasmid is found in virulent isolates of shigella sp. and encodes functions essential for invasion of mammalian cells. to identify plasmid sequences necessary for invasion, we isolated a series of tn5 insertions in pwr100, the virulence plasmid of shigella flexneri serotype 5. these insertions demonstrated that three separate ecori fragments of pwr100 were required for invasion of hela cells. however, the corresponding native ecori fragments, when cloned into pbr325, did not restore viru ... | 1985 | 2989179 |
| inhibition and activation of bovine heart nad-specific isocitrate dehydrogenase by atp. | in the absence of added calcium, inhibition of nad-specific isocitrate dehydrogenase by atp occurred without adp (i0.5 = 1.8 mm) and with 0.2 mm adp3- (i0.5 = 1.0 mm) at subsaturating substrate concentrations at ph 7.4. inhibition by atp was competitive with nad+ in the presence and absence of adp and was not reversed by magnesium citrate. no reversal of atp inhibition by free ca2+ was observed in the presence of adp (0.2 mm). however, when adp was absent, increasing ca2+ first caused progressiv ... | 1985 | 2990340 |
| bowenoid papulosis. presence of human papillomavirus (hpv) structural antigens and of hpv 16-related dna sequences. | this study reviews 39 cases of anogenital bowenoid papulosis lesions in 22 individuals of both sexes that were analyzed clinically, histologically, immunocytochemically, and virologically. macroscopically, three different types of lesions were demonstrated: erythematous macules; papules (lichenoid and/or pigmented papules); and leukoplakialike lesions. microscopically, bowenoid papulosis fulfills the criteria of a squamous cell carcinoma in situ. much like oral precancers, three distinct growth ... | 1985 | 2990353 |
| [survival of aujeszky's disease virus in infected pig slurry]. | it has been demonstrated that after experimental infection of pig slurry from the space under the slatted floor (infection dose of 10(6)pfu per ml), the aujeszky's disease virus (adv) survived for 72 hours at the temperature of 15 degrees c and at ph 6.5, but was inactivated after 96 hours. when technologically treated pig slurry from the storage tanks was saturated with water and infected with adv at the dose of 10(5)pfu per ml, the virus survived for 23 days when kept at 15 degrees c and 4 deg ... | 1985 | 2992149 |
| rates of cholesterol synthesis and low-density lipoprotein uptake in the adrenal glands of the rat, hamster and rabbit in vivo. | the absolute rate of cholesterol acquisition from de novo synthesis and from receptor-dependent and receptor-independent low-density lipoprotein (ldl) uptake was determined in the adrenal glands of the rat, hamster and rabbit under in vivo conditions. the rate of incorporation of [3h]water into cholesterol in the adrenal gland was much higher in the hamster (1727 nmol/h per g) and rabbit (853 nmol/h per g) than in the rat (71 nmol/h per g). assuming that 23 atoms of 3h are incorporated into the ... | 1985 | 2992599 |
| suicide vector for transposon mutagenesis in pseudomonas solanacearum. | a suicide vector was constructed by cloning the transfer genes of the wide-host-range (incw group) plasmid r388 into the bamhi site of pbr325. this plasmid can deliver tn5 into pseudomonas solanacearum at frequencies ranging from 10(-6) to 10(-9) per recipient. | 1985 | 2993241 |
| amino acid sequence of acylphosphatase from porcine skeletal muscle. | the amino acid sequence of acylphosphatase from porcine skeletal muscle was determined. it consists of 98 amino acid residues with n-acetylserine at the amino (n)-terminus: ac-ser-thr-ala-arg-pro-leu-lys-ser-val-asp-tyr-glu-val-phe-gly -arg-val-gln-gly-val-cys-phe-arg-met-tyr-thr-glu-asp-glu-ala-arg-lys-ile -gly-val-val-gly-trp-val-lys-asn-thr-ser-lys-gly-thr-val-thr-gly-gln -val-gln-gly-pro-glu-glu-lys-val-asn-ser-met-lys-ser-trp-leu-ser-lys -ile-gly-ser-pro-ser-ser-arg-ile-asp-arg-thr-asn-phe- ... | 1985 | 2993259 |
| the biologic significance of the mixed lymphocyte kidney culture in humans. | the mixed lymphocyte kidney culture (mlkc) in humans has been studied in normal and abnormal clinical conditions. human renal cortical cells were extracted by collagenase treatment from the kidneys of "normal" heart-beating cadaver organ donors (n = 13), patients with end-stage renal disease (esrd) at pretransplant bilateral nephrectomy and splenectomy (n = 13), and from irreversibly rejected renal allografts at the time of graft nephrectomy (n = 5). proliferation of peripheral blood t lymphocyt ... | 1985 | 2996186 |
| [the virus causing human t-cell lymphomas-leukemias]. | 1985 | 2996236 | |
| the e7-associated cell-surface antigen: a marker for the 11p13 chromosomal deletion associated with aniridia-wilms tumor. | unbalanced interstitial deletions of the p13 region of human chromosome 11 have been associated with congenital hypoplasia or aplasia of the iris, mental retardation, ambiguous genitalia, and predisposition to wilms tumor of the kidney. utilizing somatic cell hybrids containing either the normal or abnormal chromosome 11 from a child with wilms tumor and aniridia, we previously mapped the e7 cell-surface antigen to the 11p1300-to-11p15.1 region. to localize even further the site of this antigen ... | 1985 | 2996335 |
| acquired immune deficiency syndrome. a perspective for the medical practitioner. | aids is a new disease process with complications and management problems unlike anything ever seen before. an attempt has been made to summarize the available information about its various clinical presentations and how to manage them. subjects covered will include associated malignancies, infections, transmissibility, prodromal states, and infection control issues. | 1985 | 2997554 |
| pyrimidinones. 1. 2-amino-5-halo-6-aryl-4(3h)-pyrimidinones. interferon-inducing antiviral agents. | interferon induction and antiviral activity was discovered with 2-amino-5-bromo-6-phenyl-4(3h)-pyrimidinone. an analogue study incorporating a series of 2-amino-5-substituted-6-arylpyrimidinones revealed that the most potent interferon inducers were mono- and difluorophenyl analogues. these same analogues were also potent antiviral agents against semliki forest virus and herpes simplex type 1. in addition the monomethoxyphenyl analogues were potent antiviral agents but weak interferon inducers. ... | 1985 | 2999405 |
| elicitation of conspecific attack or defense in the male rat by intraventricular injection of a gaba agonist or antagonist. | the involvement of central gabaergic mechanisms in the control over offensive and defensive behaviours in the rat was studied using intracerebroventricular injections (5 microliter) of a gaba agonist (thip) or a gaba antagonist (bicuculline methiodide). intracerebroventricular injections of thip (1.25 and 2.5 micrograms) induced attacks and offensive sideways towards an untreated partner, in animals placed in a neutral area where no aggressive reactions occur in controls. social approach behavio ... | 1985 | 2999844 |
| elicitation of conspecific attack or defense in the male rat by intraventricular injection of a gaba agonist or antagonist. | the involvement of central gabaergic mechanisms in the control over offensive and defensive behaviours in the rat was studied using intracerebroventricular injections (5 microliter) of a gaba agonist (thip) or a gaba antagonist (bicuculline methiodide). intracerebroventricular injections of thip (1.25 and 2.5 micrograms) induced attacks and offensive sideways towards an untreated partner, in animals placed in a neutral area where no aggressive reactions occur in controls. social approach behavio ... | 1985 | 2999844 |
| bronchiolar and alveolar lesions in the pathogenesis of crocidolite-induced pulmonary fibrosis in mice. | asbestosis is generally considered to result in restrictive pulmonary disease associated with interstitial fibrosis. recently, however, attention has focused upon bronchiolar lesions and concomitant obstruction to air flow. the responses of conducting airways and alveoli were studied over a 20 week period following instillation of crocidolite to mice. the location of the lesions and the sequential inflammatory changes were studied by bronchoalveolar lavage, light and electron microscopy; regener ... | 1985 | 3003309 |
| [the kallikrein-kinin system in acute peritonitis and methods of correcting its disorders]. | in experimental fecal peritonitis in 140 albino rats the kallikrein-kinin system was shown to undergo the following changes: its inhibition during the first day was followed by an activation on the second day and inanition on the third day. under conditions of hyperbaric oxygenation the normalizing effect on the kinin system was observed during two days. the application of salicylates against the background of hbo was shown to enhance the normalizing effect which lasted for three days. | 1985 | 3004000 |
| fluctuation of specific iga antibodies in human milk. | the concentration of secretory iga and the levels of iga specific antibodies against escherichia coli labile-toxin, shigella flexneri 6, and rotaviruses were determined in milk samples obtained serially from women during the first 16 weeks postpartum. the mean concentration of secretory iga followed the expected pattern; the levels of specific antibodies fluctuated in an unpredictable manner and independently of milk secretory iga content, becoming undetectable in many instances. under some circ ... | 1985 | 3004100 |
| flaviviridae. | the family flaviviridae comprises the genus flavivirus, which contains 65 related species and two possible members. they are small, enveloped rna viruses (diameter 45 nm) with peplomers comprising a single glycoprotein e. other structural proteins are designated c (core) and m (membrane-like). the single strand of rna is infectious and has a molecular weight of about 4 x 10(6) and an m7g 'cap' at the 5' end but no poly(a) tract at the 3' end; it functions as the sole messenger. the gene sequence ... | 1985 | 3000978 |
| relationship of acth1-39-immunostained fibers and magnocellular neurons in the paraventricular nucleus of rat hypothalamus. | dual antigen immunocytochemical staining procedures were used in the same tissue section to determine the distribution of acth immunostained fibers and varicosities within the magnocellular and parvocellular divisions in the paraventricular nucleus (pvn) of rat hypothalamus and elucidate its anatomical relationship to vasopressin (vp) and oxytocin (oxy)-containing neurons. double immunostained preparations using glucose oxidase-antiglucose oxidase complex combined with pap complex to visualize t ... | 1985 | 3001668 |
| characterization of electron transfer and proton translocation activities in trypsin-treated bovine heart mitochondrial cytochrome c oxidase. | bovine heart mitochondrial cytochrome c oxidase has been treated with trypsin in order to investigate the role of components a, b, and c (nomenclature of capaldi) in cytochrome c binding, electron transfer, and proton-pumping activities. cytochrome c oxidase was dispersed in nondenaturing detergent solution (b. ludwig, n. w. downer, and r. a. capaldi (1979) biochemistry 18, 1401) and treated with trypsin. this treatment inhibited electron transfer activity by 9% when compared to a similarly trea ... | 1985 | 3002279 |
| [a new serotype of shigella flexneri in czechoslovakia?]. | 1985 | 3155665 | |
| synthesis of new polyoxin derivatives and their activity against chitin synthase from candida albicans. | two analogues of l-alanylpolyoxin c with a modified peptide bond were synthesized and tested for inhibition of chitin synthase in candida albicans. n-methylation of the peptide bond (compound 13) or the replacement of it by nh2ch2 (compound 9) led to loss of activity in the enzyme assay. a novel analogue (compound 5) of nikkomycin was synthesized from uracil polyoxin c and (2s,3r)-3-hydroxyhomotyrosine, a component of echinocandin c. despite high activity in the chitin synthase assay, 5 had no i ... | 1985 | 3156247 |
| distribution of sensory receptors in the flexor carpi radialis muscle of the cat. | the structures and distribution of encapsulated muscle receptors were examined in serial transverse sections of flexor carpi radialis in the adult cat. four types of receptors (muscle spindles, golgi tendon organs, paciniform, and pacinian corpuscles) were identified. their structures resembled those encountered in other limb muscles. pacinian corpuscles were rare and occurred only in the external fascial coat of the muscle near its origin. the other three receptor types were distributed in an u ... | 1985 | 3156250 |
| expression of laminin receptor in normal and carcinomatous human tissues as defined by a monoclonal antibody. | it has been hypothesized that epithelial and endothelial cells interact with the laminin component of basement membranes via a cell surface laminin receptor molecule. it has also been proposed that the expression of this molecule may be involved in the invasion of carcinoma cells from their tissue of origin and their subsequent penetration through blood vessel basement membranes. we report here the use of a monoclonal antibody, lr-3, to define the expression of laminin receptor in normal, dyspla ... | 1985 | 3157447 |
| characterization of protein kinase c and its role in catecholamine secretion from bovine adrenal-medullary cells. | protein kinase c activity towards exogenous histone was detected in a cytosolic fraction of bovine adrenal medulla. the enzyme was dependent on ca2+ and phosphatidylserine for its activity, with half-maximal activation being achieved at approx. 18 microm free ca2+ and 8 micrograms of phosphatidylserine/ml. both diolein and 4 beta-phorbol 12-myristate 13-acetate (tpa) decreased the ca2+ requirement of the enzyme, half-maximal activation being obtained at approx. 12 microm and 9 microm free ca2+ r ... | 1985 | 3159383 |
| synthesis of aerobactin and a 76,000-dalton iron-regulated outer membrane protein by escherichia coli k-12-shigella flexneri hybrids and by enteroinvasive strains of escherichia coli. | one of the chromosomal segments associated with the virulence of shigella flexneri and transferred to escherichia coli k-12 by conjugation has been shown to code for the production of aerobactin and for the synthesis of an iron-regulated 76,000-dalton (76k) outer membrane protein. analysis of various e. coli k-12-s. flexneri transconjugants showed that the genes involved with the synthesis of aerobactin and with the production of the 76k protein were linked to the mtl region of the s. flexneri c ... | 1985 | 3159680 |
| metabolism of thymoxamine. i. studies with 14c-thymoxamine in rats. | thymoxamine is rapidly and completely absorbed in rats. it is a prodrug which does not enter the systemic circulation in its unchanged form. after either oral or intravenous administration it undergoes rapid and intense metabolism involving four biotransformation reactions: enzymatic hydrolysis to the corresponding phenol (metabolite i), monodemethylation to metabolite ii, sulfate conjugation of i and ii (metabolites iii and iv) and conjugation of i and ii with glucuronic acid (metabolites v and ... | 1985 | 3161738 |
| shigella flexneri type 2 infection in captive nonhuman primates. | 1985 | 17422604 | |
| [neonatal heart rate variability]. | 1985 | 261135 | |
| [the kansai area midwives enthusiastically supporting breast feeding. encounter with the okeya technic for breast feeding and reinforced professional standing of midwifery. a discussion]. | 1985 | 261123 | |
| a serological assay for the detection of cell surface receptors of nerve growth factor. | when single-cell suspensions prepared from embryonic day 8 (e8) chick sensory ganglia are incubated with nerve growth factor (ngf), anti-ngf antiserum, and complement, an ngf-dependent cytotoxic kill of 20 (+/- 3)% of the ganglia cells is observed. this percentage is increased by a factor of two when only the neuronal cells are tested. no kill is observed on the nonneuronal cell population representing 50% of the ganglia dissociate. when e8 sensory ganglia cells are cultured in the presence of n ... | 1985 | 219297 |
| [radiofrequency thermocoagulation in occipital spondylotic neuralgia]. | 1985 | 262059 | |
| the anatomy of a smile. | the anatomy of the smile is an integral part of dentistry. its understanding involves close scrutiny of all elements of the oral region. it is not enough to establish the size of teeth based on the high and low lip lines, size of the mouth, and a shade to blend with the age and complexion. to create a harmonious smile the dentist must maintain or create the normal curvature of the lips, proper exposure of the red zone of the lips, an undistorted philtrum, and undisturbed nasolabial grooves. thes ... | 1985 | 271728 |
| aorta-coronary bypass grafting for takayasu's aortitis. | 1985 | 1346542 |