Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
screening bactericidal effect of pectobacterium carotovorum subsp. carotovorum strains against causal agent of potato soft rot.this study focuses on the potential of pectobacterium carotovorum subsp. carotovorum (pcc) strains producing bacteriocin as a tool to control potato soft rot disease. thirty out of 48 purified bacterial strains were characterized as pcc using specific pcr and phenotypic tests. the pathogenicity and pectate degrading assays were recorded positive for 13 strains. bacteriocin typing clustered producers into three groups according to their antimicrobial spectra. majority of the producers except stra ...201626523939
inhibition of microorganisms involved in deterioration of an archaeological site by silver nanoparticles produced by a green synthesis method.the citadel, part of the pre-hispanic city of teotihuacan and listed as a world heritage site, harbors irreplaceable archaeological walls and murals. this city was abandoned by the 7th century and its potential deterioration represents a noteworthy loss of the world's cultural heritage. this research consisted of isolation and identification of bacteria and fungi contributing to this deterioration from walls of a pre-hispanic city. in addition, silver nanoparticles (agnp) produced, using a green ...201627015961
activity of flavanones isolated from rhododendron hainanense against plant pathogenic fungi.in a search for naturally occurring antimicrobial compounds in medicinal plants and herbs, seven flavanones were isolated from the aerial parts of rhododendron hainanense and were tested for their antimicrobial activities against six bacteria and six plant pathogenic fungi. within the series of flavanones tested, farrerol (1) displayed moderate antibacterial activities against bacillus cereus, b. subtilis, pseudomonas aeruginosa, staphylococcus aureus, escherichia coli and erwinia carotovora, wi ...201627319130
production of rhamnolipids and diesel oil degradation by bacteria isolated from soil contaminated by petroleum.biosurfactants are microbial secondary metabolites. the most studied are rhamnolipids, which decrease the surface tension and have emulsifying capacity. in this study, the production of biosurfactants, with emphasis on rhamnolipids, and diesel oil degradation by 18 strains of bacteria isolated from waste landfill soil contaminated by petroleum was analyzed. among the studied bacteria, gram-positive endospore forming rods (39%), gram positive rods without endospores (17%), and gram-negative rods ...201626588432
expression of pr-protein genes and induction of defense-related enzymes by bacillus subtilis cbr05 in tomato (solanum lycopersicum) plants challenged with erwinia carotovora subsp. carotovora.the present study was aimed to evaluate the effectiveness of a biocontrol agent bacillus subtilis cbr05 for control of soft rot disease (erwinia carotovora subsp. carotovora) in tomato, and the possible mechanisms of its resistance induction have been investigated under pot conditions. results showed that plants inoculated with b. subtilis cbr05 had lower disease incidence (36%). a significant increase in superoxide dismutase, catalase, peroxidase, and polyphenol oxidase activities was observed ...201627405462
effects of an inducible aiia gene on disease resistance in eucalyptus urophylla × eucalyptus grandis.n-acyl-homoserine lactones (ahls) are metabolites of mostly gram-negative bacteria and are critical signaling molecules in bacterial quorum-sensing systems. at threshold concentrations, ahls can activate the expression of pathogenic genes and induce diseases. therefore, reducing ahl concentrations is a key point of disease control in plants. ahl-lactonase, which is expressed by aiia, is widespread in bacillus sp and can hydrolyze ahls. in the present study, we cloned aiia from bacillus subtilis ...201626905275
two novel antimicrobial defensins from rice identified by gene coexpression network analyses.defensins form an antimicrobial peptides (amp) family, and have been widely studied in various plants because of their considerable inhibitory functions. however, their roles in rice (oryza sativa l.) have not been characterized, even though rice is one of the most important staple crops that is susceptible to damaging infections. additionally, a previous study identified 598 rice genes encoding cysteine-rich peptides, suggesting there are several uncharacterized amps in rice. we performed in si ...201627527801
biochemical characterization and immobilization of erwinia carotovoral-asparaginase in a microplate for high-throughput biosensing of l-asparagine.l-asparaginases (l-asnase, e.c. 3.5.1.1) catalyze the conversion of l-asparagine to l-aspartic acid and ammonia. in the present work, a new form of l-asnase from a strain of erwinia carotovora (ecal-asnase) was cloned, expressed in escherichia coli as a soluble protein and characterized. the enzyme was purified to homogeneity by a single-step procedure comprising ion-exchange chromatography. the properties of the recombinant enzyme were investigated employing kinetic analysis and molecular model ...201627542748
l-asparaginase as a critical component to combat acute lymphoblastic leukaemia (all): a novel approach to target all.l-asparaginase, an anti-leukaemic drug that has been approved for clinical use for many years in the treatment of childhood acute lymphoblastic leukaemia (all), is obtained from bacterial origin (escherichia coli and erwinia carotovora). the efficacy of l-asparaginase has been discussed for the past 40 years, and an ideal substitute for the enzyme has not yet been developed. the early clearance from plasma (short half-life) and requirement for multiple administrations and hence frequent physicia ...201626698391
efficacies of quorum sensing inhibitors, piericidin a and glucopiericidin a, produced by streptomyces xanthocidicus kpp01532 for the control of potato soft rot caused by erwinia carotovora subsp. atroseptica.to discover potential inhibitors of the quorum sensing (qs) system, a library of microbial culture extracts was screened with chromobacterium violaceumcv026 strain. the culture extract of streptomyces xanthocidicus kpp01532 contained quorum-sensing inhibitors (qsis) of the cv026 strain. the active constituents of the culture extract of strain kpp01532 were purified using a series of chromatographic procedures, and based on data from nmr and mass spectroscopy, piericidin a and glucopiericidin a w ...201626856451
specific detection of pectobacterium carotovorum by loop-mediated isothermal amplification.potatoes are an important agroeconomic crop worldwide and maceration diseases caused by pectolytic bacterial pathogens result in significant pre- and post-harvest losses. pectobacterium carotovorum shares a common host range with other pectobacterium spp. and other members of the enterobacteriaceae, such as dickeya spp. as these pathogens cannot be clearly differentiated on the basis of the symptoms they cause, improved methods of identification are critical for the determination of sources of c ...201626833881
ompf of pectobacterium carotovorum subsp. carotovorum pcc3 is required for carocin d sensitivity.carocin d is a bacteriocin produced by pectobacterium carotovorum subsp. carotovorum pcc21. carocin d inhibits the growth of p carotovorum subsp. carotovorum and closely related strains. pectobacterium carotovorum subsp. carotovorum is a causative bacterium for soft rot disease and leads to severe economic losses. bacteriocins recognize and interact with a specific membrane protein of target bacteria as a receptor. to identify the receptor responsible for carocin d recognition, mutants that unde ...201627915254
characterization of pectobacterium carotovorum proteins differentially expressed during infection of zantedeschia elliotiana in vivo and in vitro which are essential for virulence.the identification of phytopathogen proteins that are differentially expressed during the course of the establishment of an infection is important to better understand the infection process. in vitro approaches, using plant extracts added to culture medium, have been used to identify such proteins, but the biological relevance of these findings for in planta infection are often uncertain until confirmed by in vivo studies. here, we compared the proteins of pectobacterium carotovorum ssp. carotov ...201627671364
simultaneous detection of brown rot- and soft rot-causing bacterial pathogens from potato tubers through multiplex pcr.ralstonia solanacearum (smith) yabuuchi et al. and erwinia carotovora subsp. carotovora (jones) bergey et al. (pectobacterium carotovorum subsp. carotovorum) are the two major bacterial pathogens of potato causing brown rot (wilt) and soft rot diseases, respectively, in the field and during storage. reliable and early detection of these pathogens are keys to avoid occurrence of these diseases in potato crops and reduce yield loss. in the present study, multiplex polymerase chain reaction (pcr) p ...201627480266
paromomycin derived from streptomyces sp. ag-p 1441 induces resistance against two major pathogens of chili pepper.this is the first report that paromomycin, an antibiotic derived from streptomyces sp. ag-p 1441 (ag-p 1441), controlled phytophthora blight and soft rot diseases caused by phytophthora capsici and pectobacterium carotovorum, respectively, in chili pepper (capsicum annum l.). chili pepper plants treated with paromomycin by foliar spray or soil drenching 7 days prior to inoculation with p. capsici zoospores showed significant (p < 0.05) reduction in disease severity (%) when compared with untreat ...201627291677
structural determinants in bacterial 2-keto-3-deoxy-d-gluconate dehydrogenase kdud for dual-coenzyme specificity.short-chain dehydrogenase/reductase (sdr) is distributed in many organisms, from bacteria to humans, and has significant roles in metabolism of carbohydrates, lipids, amino acids, and other biomolecules. an important intermediate in acidic polysaccharide metabolism is 2-keto-3-deoxy-d-gluconate (kdg). recently, two short and long loops in sphingomonas kdg-producing sdr enzymes (nadph-dependent a1-r and nadh-dependent a1-r') involved in alginate metabolism were shown to be crucial for nadph or na ...201627028675
experimental evidence for proteins constituting virion components and particle morphogenesis of bacteriophage zf40.bacteriophage zf40 is the only currently available, temperate myoviridae phage infecting the potato pathogen pectobacterium carotovorum subsp. carotovorum. despite its unusual tail morphology, its major tail sheath and tube proteins remained uncharacterized after the initial genome annotation. using esi tandem mass-spectrometry, 24 structural proteins of the zf40 virion were identified, with a sequence coverage ranging between 15.8% and 87.8%. the putative function of 16 proteins could be elucid ...201626887841
morphological features of different polyploids for adaptation and molecular characterization of cc-nbs-lrr and lea gene families in agave l.polyploidy has been widely described in many agave l. species, but its influence on environmental response to stress is still unknown. with the objective of knowing the morphological adaptations and regulation responses of genes related to biotic (lea) and abiotic (nbs-lrr) stress in species of agave with different levels of ploidy, and how these factors contribute to major response of agave against environmental stresses, we analyzed 16 morphological trials on five accessions of three species ( ...201627016883
[catalytic properties of enzymes from erwinia carotovora involved in transamination of phenylpyruvate].km for l-phenylalanine, l-glutamic acid, l-aspartic acid, and the corresponding keto acids were calculated, as well as vmax, was measured for the following pairs of substrates: l-phenylalanine-2-ketoglutarate, l-phenylalanine-oxaloacetate, l-glutamic acid-phenylpyruvate, and l-aspartic acid-phenylpyruvate for aminotransferases pati, pat2, and pat3 from erwinia carotovora catalyzing transamination of phenylpyruvate. the ping-pong bi-bi mechanism was shown for the studied aminotransferases. the su ...201623795470
penicitroamide, an antimicrobial metabolite with high carbonylization from the endophytic fungus penicillium sp. (no. 24).penicitroamide (1), a new metabolite with a new framework, was isolated from the ethyl acetate extract of the pdb (potato dextrose broth) medium of penicillium sp. (no. 24). the endophytic fungus penicillium sp. (no. 24) was obtained from the healthy leaves of tapiscia sinensis oliv. the structure of penicitroamide (1) features a bicyclo[3.2.1]octane core unit with a high degree of carbonylization (four carbonyl groups and one enol group). the chemical structure of penicitroamide (1) was elucida ...201627801845
bacterial diseases of bananas and enset: current state of knowledge and integrated approaches toward sustainable management.bacterial diseases of bananas and enset have not received, until recently, an equal amount of attention compared to other major threats to banana production such as the fungal diseases black leaf streak (mycosphaerella fijiensis) and fusarium wilt (fusarium oxysporum f. sp. cubense). however, bacteria cause significant impacts on bananas globally and management practices are not always well known or adopted by farmers. bacterial diseases in bananas and enset can be divided into three groups: (1) ...201728785275
the ribosomal protein rply is required for pectobacterium carotovorum virulence and is induced by zantedeschia elliotiana extract.pectobacterium carotovorum subsp. carotovorum strain pccs1, a bacterial pathogen causing soft rot disease of zantedeschia elliotiana (colored calla), was investigated for virulence genes induced by the host plant. using a promoter-trap transposon (mariner), we obtained 500 transposon mutants showing kanamycin resistance dependent on extract of z. elliotiana. one of these mutants, pm86, exhibited attenuated virulence on both z. elliotiana and brassica rapa subsp. pekinensis. the growth of pm86 wa ...201728853642
sde5, a putative rna export protein, participates in plant innate immunity through a flagellin-dependent signaling pathway in arabidopsis.in eukaryotes, rna silencing, mediated by small interfering rnas, is an evolutionarily widespread and versatile silencing mechanism that plays an important role in various biological processes. increasing evidences suggest that various components of rna silencing pathway are involved in plant defense machinery against microbial pathogens in arabidopsis thaliana. here, we show genetic and molecular evidence that arabidopsis sde5 is required to generate an effective resistance against the biotroph ...201728851870
the arabidopsis defensin gene, atpdf1.1, mediates defence against pectobacterium carotovorum subsp. carotovorum via an iron-withholding defence system.plant defensins (pdfs) are cysteine-rich peptides that have a range of biological functions, including defence against fungal pathogens. however, little is known about their role in defence against bacteria. in this study, we showed that the protein encoded by arabidopsis thaliana plant defensin type 1.1 (atpdf1.1) is a secreted protein that can chelate apoplastic iron. transcripts of atpdf1.1 were induced in both systemic non-infected leaves of arabidopsis thaliana plants and those infected wit ...201728835670
the sal-pap chloroplast retrograde pathway contributes to plant immunity by regulating glucosinolate pathway and phytohormone signaling.chloroplasts have a crucial role in plant immunity against pathogens. increasing evidence suggests that phytopathogens target chloroplast homeostasis as a pathogenicity mechanism. in order to regulate the performance of chloroplasts under stress conditions, chloroplasts produce retrograde signals to alter nuclear gene expression. many signals for the chloroplast retrograde pathway have been identified, including chlorophyll intermediates, reactive oxygen species, and metabolic retrograde signals ...201728703028
a novel stress response mechanism, triggered by indole, involved in quorum quenching enzyme moml and iron-sulfur cluster in muricauda olearia th120.indole, as a signal molecule, is involved in multiple physiological behavior including biofilm formation, antibiotic resistance and virulence. in this study, we demonstrated that indole was involved in iron deficient and h2o2 stress response in muricauda olearia th120. transcriptome analysis showed that totally 206 genes were regulated by exogenous indole. besides, moml-suf gene cluster, consisting of quorum quenching enzyme coding gene moml and iron-sulfur biosynthetic genes suf, were involved ...201728652609
cytr homolog of pectobacterium carotovorum subsp. carotovorum controls air-liquid biofilm formation by regulating multiple genes involved in cellulose production, c-di-gmp signaling, motility, and type iii secretion system in response to nutritional and environmental signals.pectobacterium carotovorum subsp. carotovorum [pcc (formerly erwinia carotovora subsp. carotovora)] pc1 causes soft-rot disease in a wide variety of plant species by secreting multiple pathogenicity-related traits. in this study, regulatory mechanism of air-liquid (al) biofilm formation was studied using a cytr homolog gene deletion mutant (δcytr) of pcc pc1. compared to the wild type (pcc pc1), the δcytr mutant produced fragile and significantly (p < 0.001) lower amounts of al biofilm on salt-o ...201728620360
oxygen-dependent globin coupled sensor signaling modulates motility and virulence of the plant pathogen pectobacterium carotovorum.bacterial pathogens utilize numerous signals to identify the presence of their host and coordinate changes in gene expression that allow for infection. within plant pathogens, these signals typically include small molecules and/or proteins from their plant hosts and bacterial quorum sensing molecules to ensure sufficient bacterial cell density for successful infection. in addition, bacteria use environmental signals to identify conditions when the host defenses are weakened and potentially to si ...201728612602
pectin-conjugated silica microcapsules as dual-responsive carriers for increasing the stability and antimicrobial efficacy of kasugamycin.kasugamycin is an aminoglycoside antibiotic originally isolated from streptomyces kasugaensis, which has been widely used for the management of plant diseases. however, photo-thermal instability and low efficiency limit its application. therefore, it is an urgent task to prevent unwanted loss of kasugamycin and ensure maximum bioactivity at target site. in this work, a novel formulation of kasugamycin that responds to different biological stimuli produced by pests was prepared using silica micro ...201728606541
influence of the ferric uptake regulator (fur) protein on pathogenicity in pectobacterium carotovorum subsp. brasiliense.iron is an important nutrient for the survival and growth of many organisms. in order to survive, iron uptake from the environment must be strictly regulated and maintained to avoid iron toxicity. the ferric uptake regulator protein (fur) regulates genes involved in iron homeostasis in many bacteria, including phytopathogens. however, to date, the role played by fur in the biology of pectobacterium carotovorum subsp. brasiliense (pcb1692), an important pathogen of potatoes, has not yet been stud ...201728545065
signal integration in quorum sensing enables cross-species induction of virulence in pectobacterium wasabiae.bacterial communities can sense their neighbors, regulating group behaviors in response to cell density and environmental changes. the diversity of signaling networks in a single species has been postulated to allow custom responses to different stimuli; however, little is known about how multiple signals are integrated and the implications of this integration in different ecological contexts. in the plant pathogen pectobacterium wasabiae (formerly erwinia carotovora), two signaling networks-the ...201728536283
hqia, a novel quorum-quenching enzyme which expands the ahl lactonase family.the screening of a metagenomic library of 250,000 clones generated from a hypersaline soil (spain) allowed us to identify a single positive clone which confers the ability to degrade n-acyl homoserine lactones (ahls). the sequencing of the fosmid revealed a 42,318 bp environmental insert characterized by 46 orfs. the subcloning of these orfs demonstrated that a single gene (hqia) allowed ahl degradation. enzymatic analysis using purified hqia and hplc/ms revealed that this protein has lactonase ...201728424524
drosophila larvae food intake cessation following exposure to erwinia contaminated media requires odor perception, trpa1 channel and evf virulence factor.when exposed to microorganisms, animals use several protective strategies. on one hand, as elegantly exemplified in drosophila melanogaster, the innate immune system recognizes microbial compounds and triggers an antimicrobial response. on the other hand, behaviors preventing an extensive contact with the microbes and thus reducing the risk of infection have been described. however, these reactions ranging from microbes aversion to intestinal transit increase or food intake decrease have been ra ...201728232220
molecular and biochemical characterization of recombinant cel12b, cel8c, and peh28 overexpressed in escherichia coli and their potential in biofuel production.the high crystallinity of cellulosic biomass myofibrils as well as the complexity of their intermolecular structure is a significant impediment for biofuel production. cloning of celb-, celc-encoded cellulases (cel12b and cel8c) and peh-encoded polygalacturonase (peh28) from pectobacterium carotovorum subsp. carotovorum (pcc) was carried out in our previous study using escherichia coli as a host vector. the current study partially characterizes the enzymes' molecular structures as well as their ...201728413443
genomic characterization of bacteriophage vb_pcap_pp2 infecting pectobacterium carotovorum subsp. carotovorum, a new member of a proposed genus in the subfamily autographivirinae.bacteriophage vb_pcap_pp2 (pp2) is a novel virulent phage that infects the plant-pathogenic bacterium pectobacterium carotovorum subsp. carotovorum. pp2 phage has a 41,841-bp double-stranded dna encoding 47 proteins, and it was identified as a member of the family podoviridae by transmission electron microscopy. nineteen of its open reading frames (orfs) show homology to functional proteins, and 28 orfs have been characterized as hypothetical proteins. pp2 phage is homologous to cronobacter phag ...201728409265
pcr denaturing gradient gel electrophoresis as a useful method to identify of intestinal bacteria flora in haemaphysalis flava ticks.ticks are blood-sucking ectoparasites of great medical and veterinary significance that can transmit bacteria, protozoa, fungi and viruses, causing a variety of human and animal diseases worldwide. in the present study, the intestinal bacterial flora associated with haemaphysalis flava ticks in different developmental stages were analyzed using polymerase chain reaction (pcr) and denaturing gradient gel electrophoresis (dgge). eleven distinct dgge bands were found using pcr-dgge method. sequence ...201728426403
plant growth and resistance promoted by streptomyces spp. in tomato.plant growth promoting rhizobacteria (pgpr) represent an alternative to improve plant growth and yield as well as to act as agents of biocontrol. this study characterized isolates of streptomyces spp. (stm) as pgpr, determined the antagonism of these isolates against pectobacterium carotovorum subsp. brasiliensis (pcb), evaluated the ability of stm on promoting growth and modulating the defense-related metabolism of tomato plants, and the potential of stm isolates on reducing soft rot disease in ...201728756346
wrky70 and its homolog wrky54 negatively modulate the cell wall-associated defenses to necrotrophic pathogens in arabidopsis.previous studies have identified the arabidopsis thaliana transcription factor wrky70 as a node of convergence for salicylic acid (sa) and jasmonic acid (ja)-mediated defense signal pathways and, together with its closest homolog wrky54, as a negative regulator of sa biosynthesis. here, we demonstrate that wrky70 together with wrky54 negatively affect the response of arabidopsis to the necrotrophic pathogens pectobacterium carotovorum and botrytis cinerea, but not to the hemibiotroph pseudomonas ...201728837631
a novel quorum-quenching n-acylhomoserine lactone acylase from acidovorax sp. strain mr-s7 mediates antibiotic resistance.n-acylhomoserine lactone acylase (ahl acylase) is a well-known enzyme responsible for disrupting cell-cell communication (quorum sensing) in bacteria. here, we isolated and characterized a novel and unique ahl acylase (designated macq) from a multidrug-resistant bacterium, acidovorax sp. strain mr-s7. the purified macq protein heterologously expressed in escherichia coli degraded a wide variety of ahls, ranging from c6 to c14 side chains with or without 3-oxo substitutions. we also observed that ...201728455333
a quorum sensing-defective mutant of pectobacterium carotovorum ssp. brasiliense 1692 is attenuated in virulence and unable to occlude xylem tissue of susceptible potato plant stems.pectobacterium carotovorum ssp. brasiliense 1692 (pcb1692) is an important emerging pathogen of potatoes causing blackleg in the field and soft rot during post-harvest storage. blackleg diseases involve the bacterial colonization of vascular tissue and the formation of aggregates, also known as biofilms. to understand the role of quorum sensing in vascular colonization by pcb1692, we generated a pcb1692δexpi mutant strain. inactivation of expi led to the reduced production of plant cell wall-deg ...201726788858
batch and fed-batch bioreactor studies for the enhanced production of glutaminase-free l-asparaginase from pectobacterium carotovorum mtcc 1428.the effect of dissolved oxygen (do) level and ph (controlled/uncontrolled) was first studied to enhance the production of novel glutaminase-free l-asparaginase by pectobacterium carotovorum mtcc 1428 in a batch bioreactor. the optimum level of do was found to be 20%. the production of l-asparaginase was found to be maximum when ph of the medium was maintained at 8.5 after 12 h of fermentation. under these conditions, p. carotovorum produced 17.97 u/ml of l-asparaginase corresponding to the produ ...201727070115
draft genome sequences of pectobacterium carotovorum subsp. actinidiae icmp 19971 and icmp 19972, two strains isolated from actinidia chinensis with symptoms of summer canker in south korea.pectobacterium carotovorum subsp. actinidiae is the causal agent of summer canker in kiwifruit plants in south korea. we report here the draft genome sequences of two p. carotovorum subsp. actinidiae strains, icmp 19971 and icmp 19972, which were originally isolated from actinidia chinensis with symptoms of summer canker. these genome sequences will aid in the identification of genetic traits associated with their unusual capacity to cause canker and help understanding of the threat these exotic ...201728385839
evidence-based validation of quorum quenching from lysinibacillus sphaericus and geobacillus sp. in bioremediation of oil sludge.many studies on quorum quenching focus on the discovery and characterization of novel acyl-homoserine lactonases (ahl-lactonases) because these enzymes could be used in the control of diseases caused by gram-negative bacteria. the effects of quorum quenching are also remarkable in the performance of bacterial consortia in applications such as bioremediation. in the current work, we demonstrated the presence of a potential novel ahl-lactonase-encoding locus (bsph_3377) from lysinibacillus sphaeri ...201727905820
[suppression of telomerase activity leukemic cells by mutant forms of rhodospirillum rubrum l-asparaginase].the active and stable mutant forms of short chain cytoplasmic l-asparaginase type i of rhodospirillum rubrum (rra): rra+n17, d60k, f61l, rra+n17, a64v, e67k, rra+n17, e149r, v150p, rrae149r, v150p and rrae149r, v150p, f151t were obtained by the method of site-directed mutagenesis. it is established that variants rra-n17, e149r, v150p, f151t and rrаe149r, v150p are capable to reduce an expression htert subunit of telomerase and, hence, activity of telomeres in jurkat cells, but not in cellular ly ...201728251953
bacterial expression, correct membrane targeting and functional folding of the hiv-1 membrane protein vpu using a periplasmic signal peptide.viral protein u (vpu) is a type-iii integral membrane protein encoded by human immunodeficiency virus-1 (hiv- 1). it is expressed in infected host cells and plays several roles in viral progeny escape from infected cells, including down-regulation of cd4 receptors. but key structure/function questions remain regarding the mechanisms by which the vpu protein contributes to hiv-1 pathogenesis. here we describe expression of vpu in bacteria, its purification and characterization. we report the succ ...201728225803
growth of bacterial phytopathogens in animal manures.animal manures are routinely applied to agricultural lands to improve crop yield, but the possibility to spread bacterial phytopathogens through field fertilization has not been considered yet. we monitored 49 cattle, horse, swine, sheep or chicken manure samples collected in 14 polish voivodeships for the most important plant pathogenic bacteria - ralstonia solanacearum (rsol), xanthomonas campestris pv. campestris (xcc), pectobacterium carotovorum subsp. carotovorum (pcc), pectobacterium atros ...201728319994
native and foreign proteins secreted by the cupriavidus metallidurans type ii system and an alternative mechanism.the type ii secretion system (t2ss), which transports selected periplasmic proteins across the outer membrane, has rarely been studied in nonpathogens or in organisms classified as betaproteobacteria. therefore, we studied cupriavidus metallidurans (cme), a facultative chemilithoautotroph. gel analysis of extracellular proteins revealed no remarkable differences between wild type and t2ss mutants. however, enzyme assays revealed that native extracellular alkaline phosphatase is a t2ss substrate, ...201728119513
the expanding role of type ii secretion in bacterial pathogenesis and beyond.type ii secretion (t2s) is one means by which gram-negative pathogens secrete proteins into the extracellular milieu and/or host organisms. based upon recent genome sequencing, it is clear that t2s is largely restricted to the proteobacteria, occurring in many, but not all genera in the α-, β-, γ-, and δ- classes. prominent human and/or animal pathogens that express a t2s system(s) include acinetobacter baumannii, burkholderia pseudomallei, chlamydia trachomatis, escherichia coli, klebsiella pne ...201728264910
short oligogalacturonides induce pathogen resistance-associated gene expression in arabidopsis thaliana.oligogalacturonides (ogs) are important components of damage-associated molecular pattern (damp) signaling and influence growth regulation in plants. recent studies have focused on the impact of long ogs (degree of polymerization (dp) from 10-15), demonstrating the induction of plant defense signaling resulting in enhanced defenses to necrotrophic pathogens. to clarify the role of trimers (trimeric ogs, dp3) in damp signaling and their impact on plant growth regulation, we performed a transcript ...201728103793
bacillus volatiles adversely affect the physiology and ultra-structure of ralstonia solanacearum and induce systemic resistance in tobacco against bacterial wilt.volatile organic compounds (vocs) produced by various bacteria have significant potential to enhance plant growth and to control phytopathogens. six of the most effective antagonistic bacillus spp. were used in this study against ralstonia solanacearum (rsc) tbbs1, the causal agent of bacterial wilt disease in tobacco. bacillus amyloliquefaciens fzb42 and bacillus artrophaeus lssc22 had the strongest inhibitory effect against rsc. thirteen vocs produced by fzb42 and 10 by lssc22 were identified ...201728091587
development of a sensitive and specific polyclonal antibody for serological detection of clavibacter michiganensis subsp. sepedonicus.the quarantine bacterium clavibacter michiganensis subsp. sepedonicus (cms) causes bacterial ring rot (brr) in potato but is difficult to detect, hampering the diagnosis of this disease. elisa immunoassays have not been widely used to detect cms because commercially available anti-cms antibodies detect mainly eps-producing bacteria and can fail to detect strains that do not produce eps. in the current study, we developed a new type of polyclonal antibody that specifically detects clavibacter mic ...201728068400
Refresh
firstprev
...2122232425262728
nextlast
Displaying items 2701 - 2751 of 2751