Publications

functional characterization of sequence motifs in the transit peptide of arabidopsis small subunit of rubisco.	the transit peptides of nuclear-encoded chloroplast proteins are necessary and sufficient for targeting and import of proteins into chloroplasts. however, the sequence information encoded by transit peptides is not fully understood. in this study, we investigated sequence motifs in the transit peptide of the small subunit of the rubisco complex by examining the ability of various mutant transit peptides to target green fluorescent protein reporter proteins to chloroplasts in arabidopsis (arabido ...	2006	16384899
thyroxine affects expression of kspg-related genes, the carbonic anhydrase ii gene, and ks sulfation in the embryonic chicken cornea.	opaque chick corneas become thin and transparent from embryonic day (e)9 to e20 of incubation. thyroxine (t4) injected in ovo on e9 induces precocious transparency by e12. the present study was conducted to determine whether corneal cells differentially express genes for t4 regulation, keratan sulfate proteoglycan (kspg) synthesis, crystallins, and endothelial cell ion transporters during transparency development and whether these expressions are altered when e9 embryos are treated with t4.	2006	16384953
comparison of the usage of intravenous iloprost and nitroglycerin for pulmonary hypertension during valvular heart surgery.	pulmonary hypertension secondary to valvular heart disease is a cause of acute right heart failure during valve replacement operations. this study compares the hemodynamic effects of intravenous use of iloprost and nitroglycerin in patients with pulmonary hypertension undergoing valvular replacement surgery. we sought to determine the acceptable doses of these medications for use in surgery to decrease mean pulmonary artery pressure to <30 mmhg without causing systemic side effects. the plasma n ...	2006	16387672
a general two-step strategy to synthesize lariat rnas.	we describe a general and efficient two-step strategy for lariat rna synthesis. in the first step, a deoxyribozyme synthesizes 2',5'-branched rna. in the second step, t4 rna ligase closes the loop that completes the lariat. the loop-closure reaction can form either a natural or unnatural lariat isomer, depending on which of the two 3'-termini of the branched rna reacts with the lone 5'-end. we demonstrate two approaches to control formation of either lariat isomer. in conjunction with other rout ...	2006	16373486
morphologic comparison of cervical, thoracic, lumbar intervertebral discs of cynomolgus monkey (macaca fascicularis).	the aim was to analyze the morphological differences of the intervertebral disc and endplates at different levels. forty-five vertebral motion segments were obtained from the spine of nine 3 to 4-year-old cynomolgus monkeys (macaca fascicularis). from every spine, five discs were sectioned (c5-c6, t3-t4, t9-t10, l2-l3, l4-l5). in all the groups, tissue samples were collected and sections were stained with masson's trichrome, safranine-o and van gieson's connective tissue stain to analyze the int ...	2006	16374650
effects of selenium depletion and selenium repletion by choice feeding on selenium status of young and old laying hens.	we investigated the effect of choice feeding two diets with different selenium (se) content to young and old moderately se-deficient laying hens on serum se (sse), glutathione peroxidase (gpx), vitamin e, creatine kinase (ck), aspartate aminotransferase (asat), thyroxine (t4) and triiodothyronine (t3). each of two consecutive study parts (i and ii) with the same hens and treatments began with a 6-week baseline period (medium-se diet), followed by a 9-week depletion period (low-se or medium-se di ...	2006	16376389
structural analysis of peptides that interact with newcastle disease virus.	a peptide with the sequence ctlttklyc has previously been identified to inhibit the propagation of newcastle disease virus (ndv) in embryonated chicken eggs and tissue culture. ndv has been classified into two main groups: the velogenic group, and mesogenic with lentogenic strains as the other group based on its dissociation constants. in this study the peptide, ctlttklyc, displayed on the piii protein of a filamentous m13 phage was synthesized and mutated in order to identify the amino acid res ...	2006	16377031
gamma-irradiated recd overproducers become permanent recb-/c- phenocopies for extrachromosomal dna processing due to prolonged titration of recbcd enzyme on damaged escherichia coli chromosome.	the recbcd enzyme of escherichia coli consists of three subunits recb, recc and recd. recbcd enzyme activities are regulated by its interaction with recombination hotspot chi. biochemical and genetic evidence suggest that interaction with chi affects recd subunit, and that recd polypeptide overproduction antagonizes this interaction, suggesting that intact recd replaces a chi-modified one. we used bacteria with fragmented chromosomes due to double-strand breaks inflicted by uv and gamma-irradiat ...	2006	16377056
evaluation for surrogacy of end points by using data from observational studies: tumor downstaging for evaluating neoadjuvant chemotherapy in invasive bladder cancer.	in clinical cancer trials for evaluating neoadjuvant chemotherapy, tumor downstaging is frequently used as a surrogate end point for overall survival. we evaluated the surrogacy of tumor downstaging using data from a follow-up observational study in bladder cancer.	2006	16397035
[oral squamous cell carcinoma retrospective analysis of therapy results and prognosis by neoadjuvant, preoperative radio-chemotherapy].	we investigated the charts of 129 patients treated for oral squamous cell carcinoma (scc) in the department of oral and maxillofacial surgery, university of cologne, between 1995 and 2004. only patients treated preoperatively with combined radio-chemotherapy (carboplatin/39.6 gy) were included. the purpose of the present study was to show the therapeutic outcome and the survival rates for this regimen. the mean age of the patients was 56.6 years. male patients outnumbered female patients by 3:1. ...	2006	16397802
effects of pressure support ventilation plus volume guarantee vs. high-frequency oscillatory ventilation on lung inflammation in preterm infants.	the aim of the present study was to evaluate if high-frequency oscillatory ventilation (hfov) might reduce lung inflammation in preterm infants with infant respiratory distress syndrome (rds) in comparison with the early application of another potentially lung-protective ventilation strategy, such as pressure support ventilation plus volume guarantee (psv + vg). infants at less than 30 weeks of gestation with rds were enrolled consecutively in the study if they required mechanical ventilation, a ...	2006	16397875
thyroid morphology and function in adults with untreated isolated growth hormone deficiency.	gh influences thyroid function and anatomy. although goiter is frequent in acromegalic patients, the effects of gh deficiency (ghd) are difficult to assess, because hypopituitaric subjects who lack gh often also have a partial or complete deficit of tsh.	2006	16394080
clinical case seminar: a novel lhx3 mutation presenting as combined pituitary hormonal deficiency.	lhx3 encodes lim homeodomain class transcription factors with important roles in pituitary and nervous system development. the only previous report of lhx3 mutations described patients with two types of recessive mutations displaying combined pituitary hormone deficiency coupled with neck rigidity.	2006	16394081
a comparison of short-term changes in health-related quality of life in thyroid carcinoma patients undergoing diagnostic evaluation with recombinant human thyrotropin compared with thyroid hormone withdrawal.	thyroid carcinoma requires lifelong monitoring with serum thyroglobulin, radioactive iodine whole body scanning, and other imaging modalities. levothyroxine (l-t4) withdrawal for thyroglobulin measurement and whole body scanning increases these tests' sensitivities but causes hypothyroidism. recombinant human tsh (rhtsh) enables testing without l-t4 withdrawal.	2006	16394083
efficacy of cyto-chex blood preservative for delayed manual cd4 testing using dynal t4 quant cd4 test among hiv-infected persons in zambia.	manual cd4 tests such as dynal t4 quant (dynabeads, dynal biotech, oslo, norway) are less expensive alternatives to flow cytometry in resource-limited countries. whereas blood preservatives have proven useful for stabilizing blood samples to allow delayed cd4 testing by flow cytometry, they have not been verified for manual tests. a method for preservation of blood prior to manual cd4 testing is needed for long-distance transport or sample batching.	2006	16394848
positron emission tomography scanning is not superior to whole body multidetector helical computed tomography in the preoperative staging of colorectal cancer.	the role of positron emission tomography with the glucose analogue [18f] fluoro-2-deoxy-d-glucose (fdg-pet) in the initial staging of disease in patients with primary colorectal cancer (crc) has not been adequately assessed.	2006	16361308
evolution of hepatitis b virus in an acute hepatitis b patient co-infected with genotypes b and c.	the interactions between different genotypes of hepatitis b virus (hbv) in co-infected patients remain largely unknown, especially in acute infection. here, the evolution of hbv strains was studied in an acute, self-limited hepatitis b patient co-infected with genotypes ba (b2) and c. virological analyses were performed at four time points after admission: t1 (5 days), t2 (11 days), t3 (22 days) and t4 (260 days). a dominant-genotype change from genotype c to ba was found after anti-hbv e antige ...	2006	16361416
sectional neuroanatomy of the middle thoracic spine (t5-t8) and chest.	this is the third in a series of articles on the spine. the first reviewed the anatomy of the neck. the second reviewed the upper thoracic spine and chest (t1-t4). a fourth article is planned for the lower thoracic spine. procedures in the midthoracic spine include chest tube placement, trigger point injections, chemodenervation with botulinum toxin, video-assisted thoroscopic surgery, and spinal injections. complications include pneumothorax, hemothorax, diaphragmatic irritation, sympathetic tr ...	2006	16365594
protective role of panax ginseng extract standardized with ginsenoside rg3 against acrylamide-induced neurotoxicity in rats.	acrylamide (acr) is an industrial neurotoxic chemical that has been recently found in carbohydrate-rich foods cooked at high temperatures. acr was designated as a probable human carcinogen by iarc (1994) and usepa (1988). panax ginseng extract has efficacies such as anticancer, antihypertension, antidiabetes and antinociception. the objective of the current study is to evaluate the protective effects of panax ginseng extract against acr-induced toxicity in rats. sixty adult sprague dawley female ...	2006	16389659
[surgical treatment for 217 patients with bladder transitional carcinoma].	bladder transitional cell carcinoma (tcc) is the most common cancer among urogenital neoplasms. surgical operation is the most effective therapy for tcc. this study was to explore surgical treatment for tcc and its clinical significance.	2006	16405754
dose-dependent reductions in spatial learning and synaptic function in the dentate gyrus of adult rats following developmental thyroid hormone insufficiency.	thyroid hormones are critical for the development and maturation of the central nervous system. although somatic and neurological effects are well documented following severe thyroid hormone deprivation, much less is known of the functional consequences of moderate levels of hormone insufficiency. we have previously demonstrated that severe thyroid hormone reductions in the postnatal period are associated with impairments in synaptic transmission in the dentate gyrus. the present study was perfo ...	2006	16406011
studies on cleavage of dna by n-phosphoryl branched peptides.	it was found that nalpha,nepsilon-di[n-(o,o-diisopropyl)phosphoryl-l-leucy]-l-lysyl-methyl ester (1) and nalpha,nepsilon-di[n-(o,o-diisopropyl)phosphoryl-l-phenylalanyl]-l-lysyl-methyl ester (2) could cleave supercoiled dna such as puc19 efficiently in 40 mm britton-robinson buffer. the cleavage activities for both were investigated by agarose gel electrophoresis. the t4 ligase experiments implied that the cleavage of dna occurs via a hydrolytic path. the results showed that the cleavage reactio ...	2006	16406614
cyclical changes of vertebral body heights and bone loss in healthy women after menopause.	annual changes in vertebral body heights (vhs) and lumbar bone mineral density (lbmd) were evaluated in 120 healthy pre- and post-menopausal women aged 45-74 years. subjects were divided into groups according to menstrual status and years since menopause (ysm). vertebral heights were evaluated, using radiological morphometry as the sum of anterior vertebral body heights (avhs) from t4 to l5 at baseline and exactly 12 months later. results indicate that the sum of vhs is inversely correlated with ...	2006	16406764
outcome of penile cancer in circumcised men.	we previously reported on a group of patients with post-circumcision carcinoma of the penis. we now study the long-term outcome of these patients.	2006	16406995
site-directed mutations of t4 helicase loading protein (gp59) reveal multiple modes of dna polymerase inhibition and the mechanism of unlocking by gp41 helicase.	the t4 helicase loading protein (gp59) interacts with a multitude of dna replication proteins. in an effort to determine the functional consequences of these protein-protein interactions, point mutations were introduced into the gp59 protein. mutations were chosen based on the available crystal structure and focused on hydrophobic residues with a high degree of solvent accessibility. characterization of the mutant proteins revealed a single mutation, y122a, which is defective in polymerase bindi ...	2006	16407253
new rfp- and pes213-derived tools for analyzing symbiotic vibrio fischeri reveal patterns of infection and lux expression in situ.	genetically altered or tagged vibrio fischeri strains can be observed in association with their mutualistic host euprymna scolopes, providing powerful experimental approaches for studying this symbiosis. two limitations to such in situ analyses are the lack of suitably stable plasmids and the need for a fluorescent tag that can be used in tandem with green fluorescent protein (gfp). vectors previously used in v. fischeri contain the p15a replication origin; however, we found that this replicon i ...	2006	16391121
findings on 18f-fdg pet scans after neoadjuvant chemoradiation provides prognostic stratification in patients with locally advanced rectal carcinoma subsequently treated by radical surgery.	predicting outcome after aggressive therapy for advanced rectal cancer remains difficult. (18)f-fdg pet has emerged as a valid method for predicting patient outcomes after therapy in an increasing number of cancers. we evaluated the prognostic information obtained from the degree of change in tumor (18)f-fdg pet uptake induced by chemoradiation before radical curative surgery in patients with t3/t4 rectal cancer.	2006	16391182
non-thyroidal illness syndrome is a manifestation of hypothalamic-pituitary dysfunction, and in view of current evidence, should be treated with appropriate replacement therapies.	this article documents the role of hypothalamic hypothyroidism and decreased t4-->t3 conversion as the cause of low t4 and t3 in non-thyroidal illness syndrome (ntis). this article also presents the arguments for administration of replacement triiodothyronine (t3) and thyroxine (t4) hormone in patients who have ntis. it is impossible to be certain at this time that it is beneficial to replace hormone, or whether this could be harmful. only a prospective study will be adequate to prove this point ...	2006	16399020
selection of patients with gastric adenocarcinoma for laparoscopic staging.	to refine selection criteria for laparoscopic staging of gastric adenocarcinoma, preoperatively available clinical and radiologic factors that may predict the risk of m1 disease were investigated.	2006	16399124
cd25 expression is correlated with histological grade and response to denileukin diftitox in cutaneous t-cell lymphoma.	denileukin diftitox (ontak), a recombinant fusion protein of diphtheria toxin and ligand, il-2, binds to the il-2 receptor, is internalized, and causes cell death. denileukin diftitox was approved for the treatment of cutaneous t-cell lymphomas (ctcls) with cd25+ expression. we prospectively stained lesional skin biopsy specimens from 113 mycosis fungoides and sézary syndrome patients for activation markers cd25 and cd30 to correlate expression with clinical tumor-node metastasis (tnm) stage, hi ...	2006	16410787
type 3 deiodinase is critical for the maturation and function of the thyroid axis.	developmental exposure to appropriate levels of thyroid hormones (ths) in a timely manner is critical to normal development in vertebrates. among the factors potentially affecting perinatal exposure of tissues to ths is type 3 deiodinase (d3). this enzyme degrades ths and is highly expressed in the pregnant uterus, placenta, and fetal and neonatal tissues. to determine the physiological role of d3, we have generated a mouse d3 knockout model (d3ko) by a targeted inactivating mutation of the dio3 ...	2006	16410833
association of apoptosis-related microsatellite polymorphisms on chromosome 1q in taiwanese systemic lupus erythematosus patients.	apoptosis is important in the pathogenesis of systemic lupus erythematosus (sle). several genome-wide scan studies have suggested chromosome 1q as a genetic susceptibility locus for sle. this study investigated the association of apoptosis-related genes on chromosome 1q, fas ligand (fasl), interleukin (il)-10 and poly(adp-ribose) polymerase (parp), promoter microsatellite multi-allelic polymorphisms with sle susceptibility and clinical characteristics in taiwan. this study recruited 237 sle pati ...	2006	16412052
outcomes of hand-assisted laparoscopic nephrectomy in technically challenging cases.	to evaluate the outcomes of hand-assisted laparoscopic nephrectomy in patients with significant complicating clinical factors.	2006	16413330
[central venous lines in children: new trends].	central venous catheterisation under two-dimensional ultrasound (us) guidance has been proved to be quicker and safer than the classical landmark method in both adults and children. in the literature us guidance with sterile dressing of the probe is the 'gold-standard'. another way to use us is simple preoperative us location followed either by blind puncture, either by us guided puncture when difficulties are expected: small infants (<15 kg), small diameter or collapses of the vein, multiple un ...	2006	16414237
selective neck dissection for node-positive necks in patients with head and neck squamous cell carcinoma: a word of caution.	to evaluate the regional recurrence (rr) rate in a consecutive series of patients with node-positive head and neck squamous cell carcinoma (n(+) hnscc) who underwent selective neck dissection (snd) as part of their treatment in a single institution.	2006	16415434
regional differences in anatomical landmarks for placing anterior instrumentation of the thoracic spine in both normal patients and patients with adolescent idiopathic scoliosis.	a retrospective analysis of patients who had magnetic resonance imaging (mri) of the thoracic spine, comparing those with a normal straight spine and those with a right thoracic adolescent idiopathic scoliosis (ais).	2006	16418638
poly(glycoamidoamine)s for gene delivery: stability of polyplexes and efficacy with cardiomyoblast cells.	polymeric vectors have potential as nucleic acid delivery vehicles for novel gene therapy and oligonucleotide treatments for cardiovascular disease. in this report, poly(glycoamidoamine)s that contain four secondary amines and either two or four hydroxyl units in the repeat unit with d-glucarate (d4), meso-galactarate (g4), d-mannarate (m4), and l-tartarate (t4) stereochemistry have been investigated for their pdna-binding affinity, dnase protection effect, and polyplex stability in the presence ...	2006	16417257
a case of bone metastasis of colon cancer that markedly responded to s-1/cpt-11 combination chemotherapy and became curable by resection.	an oral combined fluoropyrimidine anticancer drug, tegafur/gimeracil/oteracil potassium (s-1), has recently been used alone or in combination for colon cancer.	2006	16417646
differences in clinical characteristics and disease-free survival for latino, african american, and non-latino white men with localized prostate cancer: data from capsure.	few studies of ethnicity and prostate cancer have included latino men in analyses of baseline clinical characteristics, treatment selection, and disease-free survival (dfs). the present study examines the impact of latino ethnicity on these parameters in a large, multiinstitutional database of men with prostate cancer.	2006	16400651
comprehensive study of urinary cortisol metabolites in hyperthyroid and hypothyroid patients.	to further analyse the significance and mutual relationship of thyroid function-linked alterations in cortisol metabolism that have been separately and variously reported.	2006	16402926
investigations of thyroid hormones and antibodies based on a community health survey: the busselton thyroid study.	overt or subclinical thyroid dysfunction is common within the community, yet the significance of subtle anomalies in thyroid function tests remains contentious. the aims of this study were to: (a) establish reference intervals for serum-free thyroxine (ft4), thyroid-stimulating hormone (tsh) and thyroid antibodies (antithyroperoxidase, tpoab and antithyroglobulin, tgab) in the busselton community of south-western western australia; and (b) determine the prevalence of thyroid hormone anomalies in ...	2006	16402936
crystal structure of a dna polymerase sliding clamp from a gram-positive bacterium.	sliding dna clamps are processivity factors that are required for efficient dna replication. dna polymerases maintain proximity to nucleic acid templates by interacting with sliding clamps that encircle dna and thereby link the polymerase enzyme to the dna substrate. although the structures of sliding clamps from gram-negative bacteria (e. coli), eukaryotes, archaea, and t4-like bacteriophages are well-known, the structure of a sliding clamp from gram-positive bacteria has not been reported prev ...	2006	16403212
neoadjuvant interstitial high-dose-rate (hdr) brachytherapy combined with systemic chemotherapy in patients with breast cancer.	this is the first study investigating neoadjuvant interstitial high-dose-rate (hdr) brachytherapy combined with chemotherapy in patients with breast cancer. the goal was to evaluate the type of surgical treatment, histopathologic response, side effects, local control, and survival.	2006	16404517
interrenal and thyroid development in red drum (sciaenops ocellatus): effects of nursery environment on larval growth and cortisol concentration during settlement.	red drum settle into shallow seagrass meadows during the larval stage. day-night cycles in these habitats result in marked diel temperature and dissolved oxygen (do) cycles, and it is possible that extreme fluctuations influence endocrine development and growth of larvae. here, we described red drum interrenal and thyroid ontogeny and determine responses to environmental stimuli with special emphasis on settlement to explore possible role of hormones as mediator of directive environmental factor ...	2006	16343495
comparative analysis of thyroxine distribution in ascidian larvae.	the ascidian endostyle is a mucus-secreting pharyngeal organ, it has iodine-concentrating activity and the biosynthesis of thyroid hormones has been well documented. according to our recent findings, ascidians possess thyroid hormones, which are localized in mesenchymal cells. we have studied the presence and localization of l: -thyroxine (t(4)) in ascidia malaca (traustedt), ascidiella aspersa (müller), phallusia mamillata (cuvier) and ciona intestinalis (linnaeus) larvae and its involvement in ...	2006	16331472
genomic instability analysis of urine sediment versus tumor tissue in transitional cell carcinoma of the urinary bladder.	microsatellite alterations are a common feature of neoplastic cells. our study aimed to compare the profile of microsatellite dna alterations in tumor tissue and urine sediment at 12 selected microsatellite loci in transitional cell carcinoma of the bladder, and to determine which of the 12 markers or combination of markers has potential for the non-invasive diagnosis of bladder cancer. dna alterations were examined using microsatellite markers on chromosomes 2p, 3p, 8p, 9p, 9q, 12q, 13q, 17p an ...	2006	16328066
generalized correlation for biomolecular dynamics.	correlated motions in biomolecules are often essential for their function, e.g., allosteric signal transduction or mechanical/thermodynamic energy transport. because correlated motions in biomolecules remain difficult to access experimentally, molecular dynamics (md) simulations are particular useful for their analysis. the established method to quantify correlations from md simulations via calculation of the covariance matrix, however, is restricted to linear correlations and therefore misses p ...	2006	16355416
squamous cell carcinoma of the subglottis.	a retrospective study over a 25-year period of patients with laryngeal carcinoma treated by the department of otolaryngology at wake forest university.	2006	16360814
comparison of effects of estradiol with those of octylmethoxycinnamate and 4-methylbenzylidene camphor on fat tissue, lipids and pituitary hormones.	octylmethoxycinnamate (omc) and 4-methylbenzylidene camphor (4mbc) are commercially used absorbers of ultraviolet (uv) light. in rats, they were shown to exert endocrine disrupting including uterotrophic, i.e. estrogenic effects. estrogens have also metabolic effects, therefore the impact of oral application of the two uv absorbers at 2 doses for 3 months on lipids and hormones were compared with those of estradiol-17beta (e2). e2, omc and 4mbc reduced weight gain, the size of fat depots and ser ...	2006	16368123
prognostic significance of metastasis to the highest mediastinal lymph node in nonsmall cell lung cancer.	we have tried to clarify the prognostic significance of metastasis to the highest mediastinal (hm) lymph node in patients with n2 lung cancer who underwent complete dissection of superior mediastinal (including hm) lymph nodes.	2006	16368383
continuous positive airway pressure breathing increases the spread of sensory blockade after low-thoracic epidural injection of lidocaine.	factors affecting the distribution of sensory blockade after epidural injection of local anesthetics remain incompletely clarified. to evaluate if increasing intrathoracic pressure affects the spread of thoracic epidural anesthesia, we randomized 20 patients who received an epidural catheter at the t7-8 or t8-9 intervertebral space into 2 groups. the control group (n = 10) received an epidural test dose of 4 ml lidocaine 2% during spontaneous breathing at ambient pressure. the continuous positiv ...	2006	16368841
testis structure and function in a nongenetic hyperadipose rat model at prepubertal and adult ages.	there are few data for hormonal levels and testis structure and function during postnatal development in rats neonatally treated with monosodium l-glutamate (msg). in our study, newborn male pups were ip injected with msg (4 mg/g body weight) every 2 d up to 10 d of age and investigated at prepubertal and adult ages. plasma levels of leptin, lh, fsh, prolactin, testosterone (t), corticosterone, and free t4 (ft4) were measured. msg rats displayed elevated circulating levels of corticosterone and ...	2006	16339210
basal and evoked levels of bioassayable growth hormone are altered by hindlimb unloading.	bioassayable growth hormone (bgh) in rats is released in large quantities from the pituitary in response to the activation of large, proprioceptive afferent fibers from fast and mixed fiber-type hindlimb musculature. we hypothesized that hindlimb unloading (hu) of adult male rats would 1) reduce the basal levels of plasma bgh, and 2) abolish stimulus-induced bgh release. rats were exposed to hu for 1, 4, or 8 wk. plasma and pituitaries were collected under isoflurane anesthesia for hormone analy ...	2006	16339349
effect of pcb 126 on hepatic metabolism of thyroxine and perturbations in the hypothalamic-pituitary-thyroid axis in the rat.	the objective of this research was to examine the time- and dose- dependent disturbances in the hypothalamic-pituitary-thyroid (hpt) axis of adult male rats administered a potent coplanar (non-ortho) pcb, 3,3',4,4',5-pentachlorobiphenyl (pcb 126). adult male sprague-dawley rats were administered a single oral bolus dose of 0, 7.5, 75, or 275 microg pcb 126/kg bw dissolved in corn oil. the rats were sacrificed periodically over 22 days. the 7.5-microg/kg dose induced hepatic ethoxyresorufin-o-dee ...	2006	16339789
evidence for differential expression of notch receptors and their ligands in melanocytic nevi and cutaneous malignant melanoma.	the notch signaling has been implicated in the regulation of self-renewal of adult stem cells and differentiation of precursors along a specific cell lineage, in normal embryonic development and organogenesis. there is also evidence that signaling through notch receptors regulate cell proliferation and cell survival in several types of cancer, with opposing results depending on tissue context. no data are available in the literature concerning modulation of the expression of notch receptors, and ...	2006	16341148
recombination of the gfp gene to the bfp gene using a man-made site-selective dna cutter.	by using the recently developed man-made dna cutter [a combination of ce(iv)/edta and two dna additives], green fluorescent protein (gfp) was converted to closely related blue fluorescent protein (bfp). the phosphodiester linkages at t196-a200 in the sense strand of gfp were hydrolyzed by the cutter, and the a1-t196 fragment in the product was selectively connected with the downstream fragment (c197-a720) of bfp by t4 dna ligase. this recombination changed three codons in the gfp gene (tgc at 19 ...	2006	16341899
fetal-to-maternal transfer of thyroid hormone metabolites in late gestation in sheep.	3,3'-diiodothyronine sulfate (t2s) derived from t3 of fetal origin is transferred to the maternal circulation and contributes significantly to the maternal urinary pool. the present study quantitatively assesses the fetal to maternal transfer of t4 metabolites compared with those of t3. labeled t4 or t3 was infused intravenously to four singleton fetuses in utero in each group at gestational age 138 +/- 3 d. maternal and fetal serum and maternal urine samples were collected hourly for 4 h and at ...	2006	16327008
prognostic value of dworak grade of regression (gr) in patients with rectal carcinoma treated with preoperative radiochemotherapy.	preoperative radiochemotherapy improves local control in locally advanced rectal cancer; however, its role in prolonging survival is still controversial. in order to better define the subset in patients who might benefit from this multimodal treatment, we have evaluated the correlation between grade of regression (gr) to preoperative treatment and disease-free survival (dfs).	2006	16317549
modification of erucic acid content in indian mustard (brassica juncea) by up-regulation and down-regulation of the brassica juncea fat ty acid elongation1 (bjfae1) gene.	in brassicas, the fatty acid elongation1 (fae1) gene product, a 3-ketoacyl-coa synthase, is the first in a 4-enzyme complex involved in the synthesis of erucic acid from oleic acid. the fae1 homologue from brassica juncea cv. pusa bold was cloned in a binary vector both in sense and antisense orientations under the control of the camv35s promoter. the recombinant binary vectors were used to transform b. juncea cv. rlm 198 via agrobacterium tumefaciens. the presence of the transgene was confirmed ...	2006	16322995
optimization of thyroxine replacement therapy after total or near-total thyroidectomy for benign thyroid disease.	total or near-total thyroidectomy is increasingly used to treat benign thyroid conditions. lifelong treatment with thyroxine (t4) is then required, but the optimal dose is difficult to predict. this study investigated factors that might predict the ideal t4 dose, with the aim of reducing delays in achieving normal thyroid function after surgery.	2006	16323163
dose-dependent transthyretin inhibition of t4 uptake from cerebrospinal fluid in sheep.	transthyretin (ttr), synthesized by the choroid plexuses (cp) has an important role in transporting thyroxine from blood to cerebrospinal fluid (csf). however, the role of ttr on thyroxine transport from csf to either blood or brain is not clear. by using the incubated isolated ovine brain tissues technique, we found the cp accumulated most 125i-t4 compared to ventricular ependymal, frontal cortex or cerebellum. the accumulation was higher in the young cp than the old. there was dose-dependent i ...	2006	16325339
synthesis and metabolism of thyroid hormones is preferentially maintained in selenium-deficient transgenic mice.	the thyroid gland is rich in selenium (se) and expresses a variety of selenoproteins that are involved in antioxidative defense and metabolism of thyroid hormones (th). se deficiency impairs regular synthesis of selenoproteins and adequate th metabolism. we recently generated mice that lack the plasma se carrier, selenoprotein p (sepp). sepp-knockout mice display decreased serum se levels and manifest growth defects and neurological abnormalities partly reminiscent of thyroid gland dysfunction o ...	2006	16322066
in vitro binding of anthrax protective antigen on bacteriophage t4 capsid surface through hoc-capsid interactions: a strategy for efficient display of large full-length proteins.	an in vitro binding system is described to display large full-length proteins on bacteriophage t4 capsid surface at high density. the phage t4 icosahedral capsid features 155 copies of a nonessential highly antigenic outer capsid protein, hoc, at the center of each major capsid protein hexon. gene fusions were engineered to express the 83-kda protective antigen (pa) from bacillus anthracis fused to the n-terminus of hoc and the 130-kda pa-hoc protein was expressed in escherichia coli and purifie ...	2006	16316672
fertilization regulates apoptosis of ciona intestinalis extra-embryonic cells through thyroxine (t4)-dependent nf-kappab pathway activation during early embryonic development.	in ciona intestinalis, the elimination of extra-embryonic test cells during early stage of development is delayed by a fertilization signal. test cells undergo a caspase-dependent apoptosis event repressed by thyroxine (t4)-activated nf-kappab. when apoptosis was experimentally blocked, the hatching stage was delayed. the incubation of unfertilized eggs with a 1-h-fertilized egg extract or purified t4 restored apoptosis in test cells at a similar timing than found in fertilized eggs. ciona expre ...	2006	16313896
purkinje cell and cerebellar effects following developmental exposure to pcbs and/or mehg.	we recently reported that rats exposed to pcbs and mehg during development were impaired on the rotating rod, a test of balance and coordination that is often indicative of cerebellar damage. in addition, developmental pcb exposure is known to dramatically reduce circulating thyroid hormone concentrations, which may have a negative impact on cerebellar development. therefore, we investigated the effects of combined pcb and mehg exposure on purkinje cells and the cerebellum. the serum and brains ...	2006	16309888
rapid modulation of trh-like peptides in rat brain by thyroid hormones.	recent identification of membrane receptors for t4, t3, 3,5-t2, and 3-iodothyronamine that mediate rapid physiologic effects of thyroid hormones suggested that such receptors may supplement the regulation of trh and trh-like peptides by nuclear t3 receptors. for this reason 200 g male sprague-dawley rats received daily i.p. injections of ptu or t4. levels of trh and trh-like peptides were measured 0, 2 h or 1, 2, 3, or 4 days later. rapid increases or decreases in trh and trh-like peptide levels ...	2006	16310891
recurrent multiple cavernous hemangiomas of the orbit in association with systemic tumors.	to report the clinical findings and management of multiple, recurrent cavernous hemangiomas of the orbit in a patient with concurrent liver involvement.	2006	16678512
a single chondroitin 6-sulfate oligosaccharide unit at ser-2730 of human thyroglobulin enhances hormone formation and limits proteolytic accessibility at the carboxyl terminus. potential insights into thyroid homeostasis and autoimmunity.	we localized the site of type d (chondroitin 6-sulfate) oligosaccharide unit addition to human thyroglobulin (htg). htg was chromatographically separated into chondroitin 6-sulfate-containing (htg-cs) and chondroitin 6-sulfate-devoid (htg-cs0) molecules on the basis of their d-glucuronic acid content. in an ample number of htg preparations, the fraction of htg-cs in total htg ranged from 32.0 to 71.6%. by exploiting the electrophoretic mobility shift and metachromasia conferred by chondroitin 6- ...	2006	16679516
endocrine evidence that silvering, a secondary metamorphosis in the eel, is a pubertal rather than a metamorphic event.	silvering (transition from yellow to silver eel) has been traditionally considered as a metamorphosis in view of the numerous morphological, physiological and behavioral changes preparing the eel for the oceanic migration. however, some changes, such as increases in gonad weight and steroidogenesis, suggest that silvering could also be considered as a pubertal event. in order to assess which endocrine axis may be involved in the induction of silvering, we compared the profiles of pituitary and p ...	2006	16679776
diagnostic and treatment challenges of inframammary crease breast carcinomas.	to review one surgeon's experience with tumours of the inframammary crease.	2006	16681539
concurrent weekly docetaxel and concomitant boost radiation therapy in the treatment of locally advanced squamous cell cancer of the head and neck.	in a phase i/ii trial, we investigated concurrent weekly docetaxel and concomitant boost radiation in patients with locally advanced squamous cell cancer of the head and neck (scchn) after induction chemotherapy.	2006	16682134
correlation of visinin-like-protein-1 expression with clinicopathological features in squamous cell carcinoma of the esophagus.	ef-hand ca(2+)-sensor proteins are key molecules for transducing ca(2+) signals into physiological answers and changes in cytosolic ca(2+) concentration control a variety of cellular responses, including proliferation, migration, and differentiation, which are relevant for tumor progression. the ca(2+)-sensor visinin-like protein-1 (vilip-1) has recently attracted major interest due to its putative tumor suppressor function. whereas vilip-1 is expressed in normal skin, it is downregulated in ski ...	2006	16683251
the triplet energy of thymine in dna.	norfloxacin (nfx) photosensitizes formation of thymine dimers (t<>t) in dna, while its n(4') acetyl derivative (anfx) does not. this is evident from the observation of single-strand breaks after enzymatic treatment with t4 endonuclease v and subsequent gel electrophoresis. the triplet energies of nfx and anfx are estimated at 273 and 268 kj/mol, respectively, on the basis of triplet-triplet energy transfer quenching by a set of biphenyl and naphthalene derivatives. hence, the triplet energy of t ...	2006	16683787
photoreversible dna condensation using light-responsive surfactants.	a means to control dna compaction with light illumination has been developed using the interaction of dna with a photoresponsive cationic surfactant. the surfactant undergoes a reversible photoisomerization upon exposure to visible (trans isomer, more hydrophobic) or uv (cis isomer, more hydrophilic) light. as a result, surfactant binding to dna and the resulting dna condensation can be tuned with light. dynamic light scattering (dls) measurements were used to follow lambda-dna compaction from t ...	2006	16683804
negative influence of delayed surgery on survival after preoperative radiotherapy in rectal cancer.	in europe, until recently the standard treatment for locally advanced rectal cancer was preoperative radiotherapy (rt). the objective of this study was to evaluate the influence on survival of intervals between diagnosis and treatment.	2006	16684088
cell wall substrate specificity of six different lysozymes and lysozyme inhibitory activity of bacterial extracts.	we have investigated the specificity of six different lysozymes for peptidoglycan substrates obtained by extraction of a number of gram-negative bacteria and micrococcus lysodeikticus with chloroform/tris-hcl buffer (chloroform/buffer). the lysozymes included two that are commercially available (hen egg white lysozyme or hewl, and mutanolysin from streptomyces globisporus or m1l), and four that were chromatographically purified (bacteriophage lambda lysozyme or lal, bacteriophage t4 lysozyme or ...	2006	16684100
[diagnosis and treatment of solitary thyroid nodule--study and evaluation in portugal].	the best diagnostic and treatment strategy for an approach to the nodular thyroid disease continues to be a controversial issue.	2006	16684479
placenta hominis protects osteoporosis in ovariectomized rats.	in china, japan, and korea, placenta hominis extracts (phes) are used clinically for the treatment of osteoporosis. the anti-osteoporotic effect of phes was studied. the trabecular bone area and thickness in ovx rats decreased by 50% from those in sham-operated rats; these decreases were completely inhibited by administration of phes for 7 weeks. osteoclast numbers and the osteoblast surface were enhanced in ovx rats, but phes had no effect on these phenomena. serum phosphorus and alkaline phosp ...	2006	16684675
dysphagia severity following chemoradiation and postoperative radiation for head and neck cancer.	the purpose of the study is to evaluate dysphagia severity following chemoradiation and postoperative radiation for head and neck cancer, and particularly the aspiration risk because of its potential life-threatening consequence.	2006	16687229
molecular mechanism of photoperiodic time measurement in the brain of japanese quail.	in most organisms living in temperate zones, reproduction is under photoperiodic control. although photoperiodic time measurement has been studied in organisms ranging from plants to vertebrates, the underlying molecular mechanism is not well understood. the japanese quail (coturnix japonica) represents an excellent model to study this problem because of the rapid and dramatic photoperiodic response of its hypothalamic-pituitary-gonadal axis. recent investigations of japanese quail show that lon ...	2006	16687304
site-specific phosphorylation and caspase cleavage differentially impact tau-microtubule interactions and tau aggregation.	the microtubule-associated protein tau is hyperphosphorylated and forms neurofibrillary tangles in alzheimer disease. additionally caspase-cleaved tau is present in alzheimer disease brains co-localized with fibrillar tau pathologies. to further understand the role of site-specific phosphorylation and caspase cleavage of tau in regulating its function, constructs of full-length tau (t4) or tau truncated at asp421 (t4c3) to mimic caspase-3 cleavage with and without site-directed mutations that mi ...	2006	16687396
escherichia coli interactions with acanthamoeba: a symbiosis with environmental and clinical implications.	the ability of acanthamoeba to feed on gram-negative bacteria, as well as to harbour potential pathogens, such as legionella pneumophila, coxiella burnetii, pseudomonas aeruginosa, vibrio cholerae, helicobacter pylori, listeria monocytogenes and mycobacterium avium, suggest that both amoebae and bacteria are involved in complex interactions, which may play important roles in the environment and in human health. in this study, acanthamoeba castellanii (a keratitis isolate belonging to the t4 geno ...	2006	16687585
kinetics of error generation in homologous b-family dna polymerases.	the kinetics of forming a proper watson-crick base pair as well incorporating bases opposite furan, an abasic site analog, have been well characterized for the b family replicative dna polymerase from bacteriophage t4. structural studies of these reactions, however, have only been performed with the homologous enzyme from bacteriophage rb69. in this work, the homologous enzymes from rb69 and t4 were compared in parallel reactions to determine the relative abilities of the two polymerases to inco ...	2006	16687658
t4 category revision enhances the accuracy and significance of stage iii breast cancer.	because of the considerable heterogeneity in breast carcinoma with noninflammatory skin involvement (t4b/stage iiib), a revision was proposed of the tnm staging system that would classify these tumors exclusively based on their tumor size and lymph node status. in the current study, the authors evaluated how implementation of this proposal will affect stage iii noninflammatory breast cancer.	2006	16688772
details of the partial unfolding of t4 lysozyme on quartz using site-directed spin labeling.		2006	16671129
direct comparison of nick-joining activity of the nucleic acid ligases from bacteriophage t4.	the genome of bacteriophage t4 encodes three polynucleotide ligases, which seal the backbone of nucleic acids during infection of host bacteria. the t4dnl (t4 dna ligase) and two rna ligases [t4rnl1 (t4 rna ligase 1) and t4rnl2] join a diverse array of substrates, including nicks that are present in double-stranded nucleic acids, albeit with different efficiencies. to unravel the biochemical and functional relationship between these proteins, a systematic analysis of their substrate specificity ...	2006	16671895
cisatracurium versus vecuronium: a comparative, double blind, randomized, multicenter study in adult patients under propofol/fentanyl/n2o anesthesia.	the aim of this study was to compare the time course characteristics of cisatracurium (c) and vecuronium (v) induced neuromuscular block (nmb) following multiple doses, allowing spontaneous complete recovery (scrt) and evaluating the influence of age.	2006	16675938
[prognostic role of microvascularization in surgically treated lung cancer].	the aim of our study was the determination of microvascularization and its prognostic significance in lung cancer patients.	2006	16617384
zinc as an antiperoxidative agent following iodine-131 induced changes on the antioxidant system and on the morphology of red blood cells in rats.	iodine-131 ((131)i) irradiation is the first line treatment for graves' disease and thyroid carcinoma. in such cases, (131)i gets accumulated in the thyroid, and is released in the form of radioiodinated triiodothyronine (t3) and tetraiodothronine (t4). various reports describe changes in the blood picture after radioiodine treatment. zinc, on the other hand, has been reported to maintain the integrity of red blood cells (rbc) under certain toxic conditions. the present study was conducted to ev ...	2006	16617389
computational mutagenesis studies of protein structure-function correlations.	topological scores, measures of sequence-structure compatibility, are calculated for all 1,881 single point mutants of the human immunodeficiency virus (hiv)-1 protease using a four-body statistical potential function based on delaunay tessellation of protein structure. comparison of the mutant topological score data with experimental data from alanine scan studies specifically on the dimer interface residues supports previous findings that 1) l97 and f99 contribute greatly to the gibbs energy o ...	2006	16617425
horseshoe lung and facio-auriculo-vertebral sequence: a previously unreported association.	we describe a case of horseshoe lung in an infant with facio-auriculo-vertebral (fav) sequence that included mild hemifacial microsomia, ear anomalies, a missing left rib, left hemivertebrae (t2-t4), and complex congenital heart disease. of the approximately 40 cases of horseshoe lung described since 1962, most are reported in association with scimitar syndrome, and only four reported cases were associated with left lung hypoplasia. none of these cases included malformations consistent with a di ...	2006	16617456
stage-associated overexpression of the ubiquitin-like protein, isg15, in bladder cancer.	bladder cancer is among the most prevalent malignancies, and is characterised by frequent tumour recurrences and localised inflammation, which may promote tissue invasion and metastasis. microarray analysis was used to compare gene expression in normal bladder urothelium with that in tumours at different stages of progression. the innate immune response gene, interferon-stimulated gene 15 kda (isg15, gip2), was highly expressed at all stages of bladder cancer as compared to normal urothelium. we ...	2006	16641915
renal cell carcinoma local recurrences: impact of surgical treatment and concomitant metastasis on survival.	to analyse the survival benefit of resecting local recurrence (lr) of renal cell carcinoma (rcc) in the presence and absence of concomitant metastasis.	2006	16643473
cloning the genes and dna binding properties of high mobility group b1 (hmgb1) proteins from the human blood flukes schistosoma mansoni and schistosoma japonicum.	the parasitic helminth schistosoma mansoni contains three hmgb proteins, hmgb1, hmgb2 and hmgb3, of primary amino acid sequences highly similar to vertebrate proteins. in this report we describe the characterization of the hmgb1 proteins and their genes from s. mansoni and schistosoma japonicum. the deduced amino acid sequences of hmgb1 proteins from both schistosome species are identical, and comprise 176 residues. the proteins contain the two evolutionarily highly conserved hmg-box domains, a ...	2006	16644144
increased serum cxcl10 in graves' disease or autoimmune thyroiditis is not associated with hyper- or hypothyroidism per se, but is specifically sustained by the autoimmune, inflammatory process.	serum cxcl10 (an interferon-gamma-inducible chemokine) levels (scxcl10) are increased in several autoimmune conditions, including graves' disease (gd) and autoimmune thyroiditis (at). longitudinal assessment of scxcl10 in autoimmune hypo- or hyperthyroidism has not yet been performed.	2006	16645011
intraoperative coagulation evaluation of ischemia-reperfusion injury in small bowel transplantation: a way to explore.	the success of intestinal transplantation is affected by the extreme susceptibility of the small bowel to ischemia-reperfusion (i/r) injury. platelet aggregation decreases after reperfusion in small intestinal ischemia and liver transplantation. thromboelastography (teg) is a coagulation test performed whole on blood. the aims of this study were to assess coagulation derangements during bowel transplantation to define appropriate modalities of intraoperative coagulation monitoring. a secondary e ...	2006	16647482
intraoperative coagulation monitoring and small bowel transplantation: a way to explore.	the existence of coagulation disorders during intestinal transplantation is getting more important for a correct anesthetic management. both a condition of hypocoagulation and hypercoagulation can happen during the intraoperative course. thromboelastography (teg), a test on whole blood coagulation, allows measurement of different phases of coagulation function. the aims of our study were to evaluate coagulation derangements during bowel transplantation using teg.	2006	16647483
surgical treatment of scoliosis in larsen syndrome with bilateral hip dislocation.	this is a report of a 12-year-old girl treated surgically for scoliosis associated with bilateral hip dislocation in larsen syndrome.	2006	16648737
prevalence and persistence of certain serologic types of streptococcus pyogenes in metropolitan tokyo.	m-typing was conducted for streptococcus pyogenes strains isolated within 1 week of admission from patients admitted to the tokyo metropolitan toshima hospital for scarlet fever in the 23-year period between 1956 and 1978. m12 was detected in the largest number of patients, followed by m6, m4, m3, and m1. t-typing was also conducted for streptococcus pyogenes strains isolated from healthy schoolchildren in three different parts of the 20-year period between 1977 and 1998. t12 was detected in the ...	2006	16648943
[determination of p53 in infiltrative primary bladder tumors as a prognostic factor for understaging].	to determine if p53 expression in patients with infiltrative bladder cancer is a prognostic factor on clinical staging and cancer specific survival.	2006	16649517