Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| experimental infection of domestic pigs with african swine fever virus lithuania 2014 genotype ii field isolate. | an experimental infection was conducted to evaluate horizontal transmission, clinical, virological and humoral response induced in domestic pigs infected with african swine fever (asf) genotype ii virus circulating in 2014 into the european union (eu). ten naive pigs were placed in contact with eight pigs experimentally inoculated with the lithuanian lt14/1490 asf virus (asfv) responsible for the first asf case detected in wild boar in lithuania in january 2014. clinical examination and rectal t ... | 2017 | 25808027 |
| rope-based oral fluid sampling for early detection of classical swine fever in domestic pigs at group level. | non-invasive sampling techniques based on the analysis of oral fluid specimen have gained substantial importance in the field of swine herd management. methodological advances have a focus on endemic viral diseases in commercial pig production. more recently, these approaches have been adapted to non-invasive sampling of wild boar for transboundary animal disease detection for which these effective population level sampling methods have not been available. in this study, a rope-in-a-bait based o ... | 2017 | 28056961 |
| dna-binding properties of the african swine fever virus pa104r, a histone-like protein involved in viral replication and transcription. | african swine fever virus (asfv) codes for a putative histone-like protein (pa104r) with extensive sequence homology to bacterial proteins that are implicated in genome replication and packaging. functional characterization of purified recombinant pa104r revealed that it binds to ssdna and dsdna over a wide range of temperatures, ph values, salt concentrations and in an atp-independent manner, with an estimated binding site size of about 14-16 nucleotides. using site-directed mutagenesis, the ar ... | 2017 | 28381576 |
| co-circulation of multiple genotypes of african swine fever viruses among domestic pigs in zambia (2013-2015). | during 2013-2015, several and severe outbreaks of african swine fever (asf) affected domestic pigs in six provinces of zambia. genetic characterization of asf viruses (asfvs) using standardized genotyping procedures revealed that genotypes i, ii and xiv were associated with these outbreaks. molecular and epidemiological data suggest that genotype ii asfv (georgia 2007/1-like) detected in northern province of zambia may have been introduced from neighbouring tanzania. also, a genotype ii virus de ... | 2017 | 28299893 |
| african swine fever virus np868r capping enzyme promotes reovirus rescue during reverse genetics by promoting reovirus protein expression, virion assembly, and rna incorporation into infectious virions. | reoviruses, like many eukaryotic viruses, contain an inverted 7-methylguanosine (m7g) cap linked to the 5' nucleotide of mrna. traditional functions of capping are to promote mrna stability, protein translation, and concealment from cellular proteins that recognize foreign rna. to address the role of mrna capping during reovirus replication, we assessed the benefits of adding the african swine fever virus np868r capping enzyme. c3p3, a fusion protein containing t7 rna polymerase and np868r, was ... | 2017 | 28298603 |
| understanding african swine fever infection dynamics in sardinia using a spatially explicit transmission model in domestic pig farms. | african swine fever virus (asfv) has been endemic in sardinia since 1978, resulting in severe losses for local pig producers and creating important problems for the island's veterinary authorities. this study used a spatially explicit stochastic transmission model followed by two regression models to investigate the dynamics of asfv spread amongst domestic pig farms, to identify geographic areas at highest risk and determine the role of different susceptible pig populations (registered domestic ... | 2017 | 28296281 |
| polyvalent 2d entry inhibitors for pseudorabies and african swine fever virus. | african swine fever virus (asfv) is one of the most dangerous viruses for pigs and is endemic in africa but recently also spread into the russian federation and the eastern border of the eu. so far there is no vaccine or antiviral drug available to curtail the infection. thus, control strategies based on novel inhibitors are urgently needed. another highly relevant virus infection in pigs is aujeszky's disease caused by the alphaherpesvirus pseudorabies virus (prv). this article reports the synt ... | 2017 | 28296132 |
| unique 5'-p recognition and basis for dg:dgtp misincorporation of asfv dna polymerase x. | african swine fever virus (asfv) can cause highly lethal disease in pigs and is becoming a global threat. asfv dna polymerase x (asfvpolx) is the most distinctive dna polymerase identified to date; it lacks two dna-binding domains (the thumb domain and 8-kd domain) conserved in the homologous proteins. asfvpolx catalyzes the gap-filling reaction during the dna repair process of the asfv virus genome; it is highly error prone and plays an important role during the strategic mutagenesis of the vir ... | 2017 | 28245220 |
| safety and immunogenicity of mammalian cell derived and modified vaccinia ankara vectored african swine fever subunit antigens in swine. | a reverse vaccinology system, vaxign, was used to identify and select a subset of five african swine fever (asf) antigens that were successfully purified from human embryonic kidney 293 (hek) cells and produced in modified vaccinia virus ankara (mva) viral vectors. three hek-purified antigens [b646l (p72), e183l (p54), and o61r (p12)], and three mva-vectored antigens [b646l, ep153r, and ep402r (cd2v)] were evaluated using a prime-boost immunization regimen swine safety and immunogenicity study. ... | 2017 | 28241999 |
| genetic assessment of african swine fever isolates involved in outbreaks in the democratic republic of congo between 2005 and 2012 reveals co-circulation of p72 genotypes i, ix and xiv, including 19 variants. | african swine fever (asf) is a devastating disease of domestic pigs. it is a socioeconomically important disease, initially described from kenya, but subsequently reported in most sub-saharan countries. asf spread to europe, south america and the caribbean through multiple introductions which were initially eradicated-except for sardinia-followed by re‑introduction into europe in 2007. in this study of asf within the democratic republic of the congo, 62 domestic pig samples, collected between 20 ... | 2017 | 28218698 |
| polymerase cross-linking spiral reaction (pclsr) for detection of african swine fever virus (asfv) in pigs and wild boars. | the study reports the development of a polymerase cross-linking spiral reaction (pclsr) for the detection of african swine fever virus (asfv) dna in blood collected from infected pigs and wild boars. the method uses 3 specifically designed primers. two outer-spiral primers comprising of 3' sequences complementary to asfv p72 gene sequence and 5'end sequences complementary to exogenous gene of black widow alpha-latrotoxin as well as additional asfv specific cross-linking primer. the method is spe ... | 2017 | 28198455 |
| identification of residues within the african swine fever virus dp71l protein required for dephosphorylation of translation initiation factor eif2α and inhibiting activation of pro-apoptotic chop. | the african swine fever virus dp71l protein recruits protein phosphatase 1 (pp1) to dephosphorylate the translation initiation factor 2α (eif2α) and avoid shut-off of global protein synthesis and downstream activation of the pro-apoptotic factor chop. residues v16 and f18a were critical for binding of dp71l to pp1. mutation of this pp1 binding motif or deletion of residues between 52 and 66 reduced the ability of dp71l to cause dephosphorylation of eif2α and inhibit chop induction. the residues ... | 2017 | 28189088 |
| biological characterization of african swine fever virus genotype ii strains from north-eastern estonia in european wild boar. | due to its impact on animal health and pig industry, african swine fever (asf) is regarded as one of the most important viral diseases of pigs. following the ongoing epidemic in the transcaucasian countries and the russian federation, african swine fever virus was introduced into the estonian wild boar population in 2014. epidemiological investigations suggested two different introductions into the southern and the north-eastern part of estonia. interestingly, outbreak characteristics varied con ... | 2017 | 28116841 |
| characterization of the interaction of african swine fever virus with monocytes and derived macrophage subsets. | african swine fever (asf) is a devastating disease for which there is no vaccine available. the asf virus (asfv) primarily infects cells of the myeloid lineage and this tropism is thought to be crucial for disease pathogenesis. a detailed in vitro characterization of the interactions of a virulent sardinian isolate (22653/14) and a tissue culture adapted avirulent strain (ba71v) of asfv with porcine monocytes, un-activated (momφ), classically (mom1) and alternatively (mom2) activated monocyte-de ... | 2017 | 28062012 |
| structural insight into african swine fever virus a179l-mediated inhibition of apoptosis. | programmed cell death is a tightly controlled process critical for the removal of damaged or infected cells. pro- and antiapoptotic proteins of the bcl-2 family are pivotal mediators of this process. african swine fever virus (asfv) is a large dna virus, the only member of the asfarviridae family, and harbors a179l, a putative bcl-2 like protein. a179l has been shown to bind to several proapoptotic bcl-2 proteins; however, the hierarchy of binding and the structural basis for apoptosis inhibitio ... | 2017 | 28053104 |
| unraveling the armor of a killer: evasion of host defenses by african swine fever virus. | african swine fever is an acute hemorrhagic disease of pigs. extensive recent spread in the russian federation and eastern europe has increased the risk to global pig production. the virus is a large dna virus and is the only member of the asfarviridae family. in pigs, the virus replicates predominantly in macrophages. we review how the virus overcomes the barriers to replication in the macrophage and the virus mechanism to inhibit key host defense pathways. | 2017 | 28031363 |
| african swine fever virus gets undressed: new insights on the entry pathway. | african swine fever virus (asfv) is a large, multienveloped dna virus composed of a genome-containing core successively wrapped by an inner lipid envelope, an icosahedral protein capsid, and an outer lipid envelope. in keeping with this structural complexity, recent studies have revealed an intricate entry program. this gem highlights how asfv uses two alternative pathways, macropinocytosis and clathrin-mediated endocytosis, to enter into the host macrophage and how the endocytosed particles und ... | 2017 | 27974557 |
| variations in clinical presentation and anatomical distribution of gross lesions of african swine fever in domestic pigs in the southern highlands of tanzania: a field experience. | african swine fever is a contagious viral disease responsible for up to 100% mortality among domestic pigs. a longitudinal study was carried out to determine the clinical presentation and anatomical distribution of gross lesions in affected pigs in mbeya region, tanzania during the 2010 to 2014 outbreaks. data were collected during clinical and postmortem examination by field veterinarians and using a structured questionnaire. a total of 118 respondents (100%) showed awareness about african swin ... | 2017 | 27915438 |
| different routes and doses influence protection in pigs immunised with the naturally attenuated african swine fever virus isolate ourt88/3. | this study compares different combinations of doses and routes of immunisation of pigs with low virulent african swine fever virus (asfv) genotype i isolate ourt88/3, including the intramuscular and intranasal route, the latter not previously tested. intranasal immunisations with low and moderate doses (10(3) and 10(4) tcid50) of ourt88/3 provided complete protection (100%) against challenge with virulent genotype i ourt88/1 isolate. only mild and transient clinical reactions were observed in pr ... | 2017 | 27908827 |
| genetically edited pigs lacking cd163 show no resistance following infection with the african swine fever virus isolate, georgia 2007/1. | african swine fever is a highly contagious, often fatal disease of swine for which there is no vaccine or other curative treatment. the macrophage marker, cd163, is a putative receptor for african swine fever virus (asfv). pigs possessing a complete knockout of cd163 on macrophages were inoculated with georgia 2007/1, a genotype 2 isolate. knockout and wild type pen mates became infected and showed no differences in clinical signs, mortality, pathology or viremia. there was also no difference fo ... | 2017 | 27898335 |
| influence of age and dose of african swine fever virus infections on clinical outcome and blood parameters in pigs. | african swine fever (asf) is a fatal disease for domestic pigs, leading to serious economic losses in countries where asf is endemic. despite extensive research, efficient vaccines against asf are lacking. since peripheral blood cells are important mediators for vaccines, we study the impact of asf on blood parameters in pigs with different ages and infected with different doses of asf virus. four different groups were studied: (1) 12 weeks of age/low virus dose; (2) 12 weeks of age/high virus d ... | 2017 | 27875662 |
| development of an updated pcr assay for detection of african swine fever virus. | due to the current unavailability of vaccines or treatments for african swine fever (asf), which is caused by african swine fever virus (asfv), rapid and reliable detection of the virus is essential for timely implementation of emergency control measures and differentiation of asf from other swine diseases with similar clinical presentations. here, an improved pcr assay was developed and evaluated for sensitive and universal detection of asfv. primers specific for asfv were designed based on the ... | 2017 | 27714502 |
| simultaneous deletion of the 9gl and uk genes from the african swine fever virus georgia 2007 isolate offers increased safety and protection against homologous challenge. | african swine fever virus (asfv) is the etiological agent of a contagious and often lethal viral disease of domestic pigs that has significant economic consequences for the swine industry. the control of african swine fever (asf) has been hampered by the unavailability of vaccines. successful experimental vaccines have been derived from naturally occurring, cell culture-adapted, or genetically modified live attenuated asfv. recombinant viruses harboring engineered deletions of specific virulence ... | 2017 | 27795430 |
| survival of african swine fever virus in excretions from pigs experimentally infected with the georgia 2007/1 isolate. | african swine fever virus (asfv) causes a lethal haemorrhagic disease of swine which can be transmitted through direct contact with infected animals and their excretions or indirect contact with contaminated fomites. the shedding of asfv by infected pigs and the stability of asfv in the environment will determine the extent of environmental contamination. the recent outbreaks of asf in europe make it essential to develop disease transmission models in order to design effective control strategies ... | 2017 | 26104842 |
| active surveillance of african swine fever in domestic swine herds in georgia, 2014. | since its introduction to the republic of georgia in 2007, african swine fever virus (asfv) has spread across the caucasus region, the russian federation, and some eastern european countries. it is assumed that large populations of naïve, domestic, free-ranging and wild pigs are vital to the transmission of the disease. since its epidemic emergence in the region in 2007, asfv has continued to circulate, which suggests that an endemic cycle has been established and is maintained by contact betwee ... | 2017 | 30160694 |
| pacmanvirus, a new giant icosahedral virus at the crossroads between asfarviridae and faustoviruses. | african swine fever virus, a double-stranded dna virus that infects pigs, is the only known member of the asfarviridae family. nevertheless, during our isolation and sequencing of the complete genome of faustovirus, followed by the description of kaumoebavirus, carried out over the past 2 years, we observed the emergence of previously unknown related viruses within this group of viruses. here we describe the isolation of pacmanvirus, a fourth member in this group, which is capable of infecting a ... | 2017 | 28446673 |
| novel pestivirus species in pigs, austria, 2015. | a novel pestivirus species was discovered in a piglet-producing farm in austria during virologic examinations of congenital tremor cases. the emergence of this novel pestivirus species, provisionally termed linda virus, in domestic pigs may have implications for classical swine fever virus surveillance and porcine health management. | 2017 | 28628456 |
| seroprevalence of economically important viral pathogens in swine populations of trinidad and tobago, west indies. | the objective of this study was to evaluate the seroprevalence and identify the strains of swine influenza virus (swiv), as well as the seroprevalence of porcine parvovirus (ppv), transmissible gastroenteritis virus (tgev), porcine reproductive and respiratory syndrome virus (prrsv), porcine respiratory coronavirus (prcv), porcine circovirus type 2 (pcv-2), and classical swine fever virus (csfv) in pigs in trinidad and tobago (t&t). blood samples (309) were randomly collected from pigs at farms ... | 2017 | 28523387 |
| investigations of pro- and anti-apoptotic factors affecting african swine fever virus replication and pathogenesis. | african swine fever virus (asfv) is a large dna virus that replicates predominantly in the cell cytoplasm and is the only member of the asfarviridae family. the virus causes an acute haemorrhagic fever, african swine fever (asf), in domestic pigs and wild boar resulting in the death of most infected animals. apoptosis is induced at an early stage during virus entry or uncoating. however, asfv encodes anti-apoptotic proteins which facilitate production of progeny virions. these anti-apoptotic pro ... | 2017 | 28841179 |
| ba71δcd2: a new recombinant live attenuated african swine fever virus with cross-protective capabilities. | african swine fever is a highly contagious viral disease of mandatory declaration to the world organization for animal health (oie). lack of available vaccines make its control difficult and thus asfv represents a major threat to the swine industry. inactivated vaccines do not confer solid protection against african swine fever virus (asfv). conversely, live attenuated viruses (lav), either naturally isolated or obtained by genetic manipulation, have demonstrated reliable protection against homo ... | 2017 | 28814514 |
| estimation of the transmission dynamics of african swine fever virus within a swine house. | the spread of african swine fever virus (asfv) threatens to reach further parts of europe. in countries with a large swine production, an outbreak of asf may result in devastating economic consequences for the swine industry. simulation models can assist decision makers setting up contingency plans. this creates a need for estimation of parameters. this study presents a new analysis of a previously published study. a full likelihood framework is presented including the impact of model assumption ... | 2017 | 28768556 |
| a proteomic insight into the midgut proteome of ornithodoros moubata females reveals novel information on blood digestion in argasid ticks. | the argasid tick ornithodoros moubata is the main african vector of the human relapsing fever agent borrelia duttoni and the african swine fever virus. together with saliva, the tick midgut forms part of the host-tick-pathogen interface, and numerous midgut proteins play key functions in the blood digestion-related process and the infection and transmission of pathogens. this work explores the composition of the midgut proteome of unfed and fed o. moubata females with the aim to complete the bio ... | 2017 | 28764815 |
| african swine fever virus infection in classical swine fever subclinically infected wild boars. | recently moderate-virulence classical swine fever virus (csfv) strains have been proven capable of generating postnatal persistent infection (pi), defined by the maintenance of viremia and the inability to generate csfv-specific immune responses in animals. these animals also showed a type i interferon blockade in the absence of clinical signs. in this study, we assessed the infection generated in 7-week-old csfv pi wild boars after infection with the african swine fever virus (asfv). the wild b ... | 2017 | 28764692 |
| evaluation of blowfly larvae (diptera: calliphoridae) as possible reservoirs and mechanical vectors of african swine fever virus. | in 2014, highly virulent african swine fever virus (asfv) was introduced into the baltic states and poland, with new cases being reported almost every week from wild boar and also from domestic pigs. contrary to initial predictions that the disease would either die out due to the high virulence of the virus strain or spread rapidly in westerly direction, the infection became endemic and spread slowly. the unexpected disease epidemiology led to the hypothesis that hitherto unconsidered factors mi ... | 2017 | 28762629 |
| vaccinia virus and cowpox virus are not susceptible to the interferon-induced antiviral protein mxa. | mxa protein is expressed in response to type i and type iii interferon and constitute an important antiviral factor with broad antiviral activity to diverse rna viruses. in addition, some studies expand the range of mxa antiviral activity to include particular dna viruses like monkeypox virus (mpxv) and african swine fever virus (asfv). however, a broad profile of activity of mxa to large dna viruses has not been established to date. here, we investigated if some well characterized dna viruses b ... | 2017 | 28727764 |
| detection of african swine fever virus in the tissues of asymptomatic pigs in smallholder farming systems along the kenya-uganda border: implications for transmission in endemic areas and asf surveillance in east africa. | the persistence of african swine fever virus (asfv) in endemic areas, with small-scale but regular outbreaks in domestic pigs, is not well understood. asfv has not been detected using conventional diagnosis in these pigs or adjacent populations of resistant african wild pigs, that could act as potential carriers during the outbreaks. however, such data are crucial for the design of evidence-based control strategies. we conducted cross-sectional (1107 pigs) and longitudinal (100 pigs) monitoring ... | 2017 | 28721858 |
| virulent strain of african swine fever virus eclipses its attenuated derivative after challenge. | african swine fever (asf) is one of the most devastating diseases affecting the swine industry worldwide. no effective vaccine is currently available for disease prevention and control. although live attenuated vaccines (lav) have demonstrated great potential for immunizing against homologous strains of african swine fever virus (asfv), adverse reactions from lav remain a concern. here, by using a homologous asfv congo strain system, we show passage-attenuated congo lav to induce an efficient pr ... | 2017 | 28691128 |
| redistribution of endosomal membranes to the african swine fever virus replication site. | african swine fever virus (asfv) infection causes endosomal reorganization. here, we show that the virus causes endosomal congregation close to the nucleus as the infection progresses, which is necessary to build a compact viral replication organelle. asfv enters the cell by the endosomal pathway and reaches multivesicular late endosomes. upon uncoating and fusion, the virus should exit to the cytosol to start replication. asfv remodels endosomal traffic and redistributes endosomal membranes to ... | 2017 | 28587154 |
| selection of differently temporally regulated african swine fever virus promoters with variable expression activities and their application for transient and recombinant virus mediated gene expression. | african swine fever virus threatens pig production worldwide due to the lack of vaccines, for which generation of both deletion and insertion mutants is considered. for development of the latter, operational asfv promoters of different temporal regulation and strengths are desirable. we therefore compared the capacities of putative promoter sequences from p72, cd2v, p30, viral dna polymerase and u104l genes to mediate expression of luciferase from transfected plasmids after activation in trans, ... | 2017 | 28502836 |
| comparative phenotypic and functional analyses of the effects of autologous plasma and recombinant human macrophage-colony stimulating factor (m-csf) on porcine monocyte to macrophage differentiation. | porcine monocyte-derived macrophages (momφ) have been employed as a model cell in numerous studies of the porcine immune system. however, the lack of a standardized method for momφ differentiation hampers the comparison of results coming from the use of different laboratory protocols. in this study we compared the use of varying concentrations of autologous plasma (10, 20 and 30% v/v) or recombinant human macrophage-colony stimulating factor (hm-csf; 50, 100, and 200ng/ml) to differentiate porci ... | 2017 | 28494933 |
| hemophagocytic lymphohistiocytosis in acute african swine fever clinic. | hemophagocytic lymphohistiocytosis (hlh) usually has been defined as the combination of a proliferation of cytologically benign, actively phagocytic macrophages in bone marrow, spleen, lymph nodes, etc. in association with fever, cytopenia, splenomegaly, and hypertriglyceridemia. hlh is often triggered by viral infection. the aim of this study was to ascertain the features of hlh involvement in african swine fever virus (asfv) (genotype ii) pathogenesis. | 2017 | 28494931 |
| african swine fever virus: a review. | african swine fever (asf) is a highly contagious viral disease of swine which causes high mortality, approaching 100%, in domestic pigs. asf is caused by a large, double stranded dna virus, asf virus (asfv), which replicates predominantly in the cytoplasm of macrophages and is the only member of the asfarviridae family, genus asfivirus. the natural hosts of this virus include wild suids and arthropod vectors of the ornithodoros genus. the infection of asfv in its reservoir hosts is usually asymp ... | 2017 | 28489063 |
| adenovirus-vectored novel african swine fever virus antigens elicit robust immune responses in swine. | african swine fever virus (asfv) is a high-consequence transboundary animal pathogen that often causes hemorrhagic disease in swine with a case fatality rate close to 100%. lack of treatment or vaccine for the disease makes it imperative that safe and efficacious vaccines are developed to safeguard the swine industry. in this study, we evaluated the immunogenicity of seven adenovirus-vectored novel asfv antigens, namely a151r, b119l, b602l, ep402rδprr, b438l, k205r and a104r. immunization of com ... | 2017 | 28481911 |
| pig traders' networks on the kenya-uganda border highlight potential for mitigation of african swine fever virus transmission and improved asf disease risk management. | we applied social network analysis to pig trader networks on the kenya-uganda border. social network analysis is a recently developed tool, which is useful for understanding value chains and improving disease control policies. we interviewed a sample of 33 traders about their experiences with trade and african swine fever (asf), analyzed the networks they generated in purchasing pigs and selling pork and their potential contribution to modulating dissemination of the asf virus (asfv). the majori ... | 2017 | 28460754 |
| development of a fluorescent asfv strain that retains the ability to cause disease in swine. | african swine fever is a contagious and often lethal disease for domestic pigs with a significant economic impact for the swine industry. the etiological agent, african swine fever virus (asfv), is a highly structurally complex double stranded dna virus. no effective vaccines or antiviral treatment are currently commercially available. we present here the development of a strain of asfv that has been shown to retain its ability to cause disease in swine, efficiently replicate in swine macrophage ... | 2017 | 28436458 |
| proposed revision to the taxonomy of the genus pestivirus, family flaviviridae. | we propose the creation of seven new species in the genus pestivirus (family flaviviridae) in addition to the four existing species, and naming species in a host-independent manner using the format pestivirus x. only the virus species names would change; virus isolates would still be referred to by their original names. the original species would be re-designated as pestivirus a (original designation bovine viral diarrhea virus 1), pestivirus b (bovine viral diarrhea virus 2), pestivirus c (clas ... | 2017 | 28786787 |
| detection of border disease virus in mexican cattle. | the genus pestivirus within flaviviridae is comprised of four recognized species, namely, bovine viral diarrhoea virus 1 (bvdv-1), bovine viral diarrhoea virus 2 (bvdv-2), border disease virus (bdv) and classical swine fever virus (csfv). bdv, while primarily infecting sheep and goats, has also been reported in cattle and wild animals. infections of sheep and goats result in economic loss due to abortions and the birth of persistently infected animals that have poor production and reduced life e ... | 2017 | 28474402 |
| a novel viewrna in situ hybridization method for the detection of the dynamic distribution of classical swine fever virus rna in pk15 cells. | classical swine fever (csf) is a highly contagious fatal infectious disease caused by classical swine fever virus (csfv). a better understanding of csfv replication is important for the study of pathogenic mechanism of csf. with the development of novel rna in situ hybridization method, quantitatively localization and visualization of the virus rna molecular in cultured cell or tissue section becomes very important tool to address these pivotal pathogenic questions. in this study, we established ... | 2017 | 28420390 |
| pre-screening of crude peptides in a serological bead-based suspension array. | most serological assays detect antibody responses in biological samples through affinity of serum antibodies for antigens provided in the assay. certain antigens, however, may be difficult to produce and/or may contain unwanted epitopes. in these cases, a practical alternative may be the use of peptides as representatives for specific epitopes. peptides can be obtained after purification in large quantities for a modest price, but screening of a large set of peptides during development may be re ... | 2017 | 28545817 |
| [expression and antiviral activity of a chimeric porcinized monoclonal antibody (chq06) against e2 protein of classical swine fever virus]. | classical swine fever (csf), one of oie-listed diseases, is a highly contagious and economically important disease of pigs. classical swine fever virus (csfv) is the causative agent of csf. the capsid (c) protein and the glycoproteins erns, e1 and e2, are structural components of the virus. e2 is the most immunogenic protein of the csfv glycoproteins, inducing neutralizing antibodies that provide protection against lethal csfv challenge. in a previous study, we developed a murine mab hq06 agains ... | 2017 | 28853251 |
| rab5 enhances classical swine fever virus proliferation and interacts with viral ns4b protein to facilitate formation of ns4b related complex. | classical swine fever virus (csfv) is a fatal pig pestivirus and causes serious financial losses to the pig industry. csfv ns4b protein is one of the most important viral replicase proteins. rab5, a member of the small rab gtpase family, is involved in infection and replication of numerous viruses including hepatitis c virus and dengue virus. until now, the effects of rab5 on the proliferation of csfv are poorly defined. in the present study, we showed that rab5 could enhance csfv proliferation ... | 2017 | 28848503 |
| the uncoupling of protease trans-cleavage and helicase activities in the pestivirus ns3. | the nonstructural protein ns3 from the flaviviridae family is a multi-functional protein that contains an n-terminal protease and a c-terminal helicase, playing essential roles in viral polyprotein processing and genome replication. here we report a full-length crystal structure of the classical swine fever virus (csfv) ns3 in complex with its ns4a protease cofactor segment (pcs) at 2.35 å resolution. the structure reveals a previously unidentified ∼2200-å(2) intra-molecular protease-helicase in ... | 2017 | 28835495 |
| epidemiology, diagnosis and control of classical swine fever: recent developments and future challenges. | classical swine fever (csf) represents a major health and trade problem for the pig industry. in endemic countries or those with a wild boar reservoir, csf remains a priority for veterinary services. surveillance as well as stamping out and/or vaccination are the principle tools of prevention and control, depending on the context. in the past decades, marker vaccines and accompanying diagnostic tests allowing the discrimination of infected from vaccinated animals have been developed. in the euro ... | 2017 | 28795533 |
| changing pattern of classical swine fever virus genogroup from classical 1.1 to emerging 2.2 in india. | classical swine fever (csf) is one of the most important viral diseases of pigs with high economic impact. the causative agent, classical swine fever virus (csfv) is a member of genus pestivirus in family flaviviredae and is structurally and antigenically related to other members of the genus. the identification of virus strains and genotypes can conveniently be used to trace the origin and patterns of virus spread, which contribut substantially in control strategies. in the present study, we ha ... | 2017 | 28770243 |
| traf6 is a novel ns3-interacting protein that inhibits classical swine fever virus replication. | classical swine fever virus (csfv) non-structural protein 3 (ns3) is a multifunctional non-structural protein that plays a major role in viral replication. however, how exactly ns3 exerts these functions remains unknown. here, we identified tumour necrosis factor receptor-associated factor 6 (traf6) as a novel ns3-interacting protein via yeast two-hybrid analysis, co-immunoprecipitation, and glutathione s-transferase pull-down assays. furthermore, we observed that traf6 overexpression significan ... | 2017 | 28751780 |
| identification of the linear ligand epitope on classical swine fever virus that interacts with porcine kidney 15 cells. | binding of the viral ligand to a specific receptor is the first step of virus entry into target cells. the envelope proteins e(rns), e1, and e2 of classical swine fever virus (csfv) are involved in the interaction with host cell receptors to mediate csfv infection. the aim of this investigation was to identify epitopes that bind to porcine kidney (pk)-15 cells to prevent csfv infection. ten peptides representing e(rns), e1, and e2 were synthesized. immunohistochemical study showed that the se24 ... | 2017 | 28725108 |
| us10, a novel npro-interacting protein, inhibits classical swine fever virus replication. | classical swine fever (csf) is a severe, febrile and highly contagious disease caused by classical swine fever virus (csfv) that has resulted in huge economic losses in the pig industry worldwide. csfv npro has been actively studied but remains incompletely understood. few studies have investigated the cellular proteins that interact with npro and their participation in viral replication. here, the yeast two-hybrid (y2h) system was employed to screen npro-interacting proteins from a porcine alve ... | 2017 | 28721853 |
| classical swine fever virus in cattle. | 2017 | 28710174 | |
| continuous passaging of a recombinant c-strain virus in pk-15 cells selects culture-adapted variants that showed enhanced replication but failed to induce fever in rabbits. | classical swine fever virus (csfv) is the etiologic agent of classical swine fever (csf), a highly contagious disease that causes significant economic losses to the swine industry. the lapinized c-strain, a widely used vaccine strain against csfv, has low growth efficiency in cell culture, thus limits the productivity in vaccine industry. in this study, a recombinant virus derived from c-strain was constructed and subjected to continuous passaging in pk-15 cells with the goal of acquiring high p ... | 2017 | 28704902 |
| a single dose of the novel chimeric subunit vaccine e2-cd154 confers early full protection against classical swine fever virus. | classical swine fever is an economically important, highly contagious disease of swine worldwide. subunit vaccines are a suitable alternative for the control of classical swine fever. however, such vaccines have as the main drawback the relatively long period of time required to induce a protective response, which hampers their use under outbreak conditions. in this work, a lentivirus-based gene delivery system is used to obtain a stable recombinant hek 293 cell line for the expression of e2-csf ... | 2017 | 28688785 |
| an immuno-chromatographic lateral flow assay (lfa) for rapid on-the-farm detection of classical swine fever virus (csfv). | classical swine fever (csf) is a highly contagious and potentially fatal disease of domestic pigs. classical swine fever is routinely diagnosed by clinical signs, serology, detection of csf virus (csfv) nucleic acid by pcr and virus isolation. most of the current csf diagnostic methods are expensive and have an extended turnaround time. in the majority of the csf endemic countries, lack of easy access to diagnostic facilities is a major problem for swine producers trying to obtain early diagnosi ... | 2017 | 28685287 |
| complex virus-host interactions involved in the regulation of classical swine fever virus replication: a minireview. | classical swine fever (csf), caused by classical swine fever virus (csfv), is one of the most devastating epizootic diseases of pigs in many countries. viruses are small intracellular parasites and thus rely on the cellular factors for replication. fundamental aspects of csfv-host interactions have been well described, such as factors contributing to viral attachment, modulation of genomic replication and translation, antagonism of innate immunity, and inhibition of cell apoptosis. however, thos ... | 2017 | 28678154 |
| efficacy of e2 glycoprotein fused to porcine cd154 as a novel chimeric subunit vaccine to prevent classical swine fever virus vertical transmission in pregnant sows. | here we evaluated the effect of double vaccination with a novel subunit marker vaccine candidate based in the csfv e2 glycoprotein fused to the porcine cd154 to prevent csfv vertical transmission. a lentivirus-based gene delivery system was used to obtain a stable recombinant hek 293 cell line for the expression of e2 fused to porcine cd154 molecule. six pregnant sows were distributed in two groups and at 64days of gestation animals numbered 1-4 (group 1) were vaccinated via intramuscular inocul ... | 2017 | 28622852 |
| recombinant swinepox virus expressing glycoprotein e2 of classical swine fever virus confers complete protection in pigs upon viral challenge. | classical swine fever (csf) is a highly contagious and serious viral disease that affects the pig industry worldwide. the glycoprotein e2 of the classical swine fever virus (csfv) can induce neutralizing antibodies, and it is widely used for novel vaccine development. to explore the development of a vaccine against csfv infections, the gene of glycoprotein e2 was inserted into the swinepox virus (spv) genome by homologous recombination. the culture titers of rspv-e2 remained at about 4.3 × 10(6) ... | 2017 | 28612010 |
| novel thioglycosyl analogs of glycosyltransferase substrates as antiviral compounds against classical swine fever virus and hepatitis c virus. | hepatitis c virus (hcv) and classical swine fever virus (csfv) are important pathogens for which new therapeutic approaches are in high demand. herein, we report the synthesis of newly designed thioglycosyl analogs of glycosyltransferase substrates which were evaluated using cell-based assays for cytotoxicity and antiviral activity against both viruses. the antiviral activity of synthesized compounds against csfv and hcv was confirmed using pseudo-plaque reduction assays where a significant arre ... | 2017 | 28601004 |
| synergistic roles of the e2 glycoprotein and 3' untranslated region in the increased genomic stability of chimeric classical swine fever virus with attenuated phenotypes. | the e2 glycoprotein and 3' untranslated region (utr) of classical swine fever virus (csfv) are virulence determinants. to investigate the synergistic roles of e2 and 3'utr for pathogenicity and genomic stability, a series of chimeric csfvs were constructed by replacing the e2 gene and/or 3'utr of virulent csfv strain shimen with the corresponding sequence of the lapinized 'chinese' strain (c-strain) using a reverse genetic approach. the in vitro growth characterization and in vivo pathogenicity ... | 2017 | 28573473 |
| a bivalent dendrimeric peptide bearing a t-cell epitope from foot-and-mouth disease virus protein 3a improves humoral response against classical swine fever virus. | three dendrimeric peptides were synthesized in order to evaluate their immunogenicity and their potential protection against classical swine fever virus (csfv) in domestic pigs. construct 1, an optimized version of a previously used dendrimer, had four copies of a b-cell epitope derived from csfv e2 glycoprotein connected to an also csfv-derived t-cell epitope through maleimide instead of thioether linkages. construct 2 was similarly built but included only two copies of the b-cell epitope, and ... | 2017 | 28571760 |
| effects of vaccination with the c-strain vaccine on immune cells and cytokines of pigs against classical swine fever virus. | the attenuated c-strain vaccine against classical swine fever virus (csfv) is one of the safest and most effective attenuated vaccines. however, little is known of the host immune response after vaccination with the c-strain vaccine. blood samples from vaccinated pigs were collected to evaluate the number of immune cells, the level of specific csfv antibody, and related cytokines induced by the vaccination of c-strain vaccine. the c-strain nucleic acid was gradually removed and specific antibody ... | 2017 | 28514189 |
| surface displaying of swine igg1 fc enhances baculovirus-vectored vaccine efficacy by facilitating viral complement escape and mammalian cell transduction. | baculovirus-mediated gene transfer has been developed as a vaccine design strategy against a number of diseases without apparent viral replication. however, it has been hampered by complement-dependent inactivation, thus hindering the in vivo application of baculovirus. a variety of approaches have been exploited to bypass the complement system in the serum. in this study, we constructed and screened a series of baculovirus vectors displaying complement interfering factors, of which a baculoviru ... | 2017 | 28499403 |
| serum metabolomic profiling of piglets infected with virulent classical swine fever virus. | classical swine fever (csf) is a highly contagious swine infectious disease and causes significant economic losses for the pig industry worldwide. the objective of this study was to determine whether small molecule metabolites contribute to the pathogenesis of csf. birefly, serum metabolomics of csfv shimen strain-infected piglets were analyzed by ultraperformance liquid chromatography/electrospray ionization time-of-flight mass spectrometry (uplc/esi-q-tof/ms) in combination with multivariate s ... | 2017 | 28496435 |
| metabolic profiles in cell lines infected with classical swine fever virus. | viruses require energy and biosynthetic precursors from host cells for replication. an understanding of the metabolic interplay between classical swine fever virus (csfv) and host cells is important for exploring the complex pathological mechanisms of classical swine fever (csf). in the current study, and for the first time, we utilized an approach involving gas chromatography coupled with mass spectrometry (gc-ms) to examine the metabolic profiles within pk-15 and 3d4/2 cells infected with csfv ... | 2017 | 28473819 |
| phylogenetic analysis of classical swine fever virus from archival formalin fixed clinical tissues reveals vietnamese origin of the isolates. | detection of classical swine fever virus (csfv) nucleic acid in archival formalin fixed tissue samples and their use for phylogenetic analysis was investigated. ten samples were examined for the presence of csfv nucleic acid by reverse transcriptase polymerase chain reaction (rt-pcr) amplification of 5'utr and e2 gene. rt-pcr was found positive for 5'utr fragment in eight samples while only one tissue samples showed amplification for e2 gene target fragment. for molecular epidemiology of the dis ... | 2017 | 28466064 |
| csfv induced mitochondrial fission and mitophagy to inhibit apoptosis. | classical swine fever virus (csfv), which causes typical clinical characteristics in piglets, including hemorrhagic syndrome and immunosuppression, is linked to hepatitis c and dengue virus. oxidative stress and a reduced mitochondrial transmembrane potential are disturbed in csfv-infected cells. the balance of mitochondrial dynamics is essential for cellular homeostasis. in this study, we offer the first evidence that csfv induces mitochondrial fission and mitophagy to inhibit host cell apoptos ... | 2017 | 28455958 |
| development of isothermal recombinase polymerase amplification assay for rapid detection of porcine circovirus type 2. | porcine circovirus virus type ii (pcv2) is the etiology of postweaning multisystemic wasting syndrome (pmws), porcine dermatitis, nephropathy syndrome (pdns), and necrotizing pneumonia. rapid diagnosis tool for detection of pcv2 plays an important role in the disease control and eradication program. recombinase polymerase amplification (rpa) assays using a real-time fluorescent detection (pcv2 real-time rpa assay) and rpa combined with lateral flow dipstick (pcv2 rpa lfd assay) were developed ta ... | 2017 | 28424790 |
| comparative characterization analysis of synonymous codon usage bias in classical swine fever virus. | classical swine fever virus (csfv) is responsible for the highly contagious viral disease of swine, and causes great economic loss in the swine-raising industry. considering the significance of csfv, a systemic analysis was performed to study its codon usage patterns. in this study, using the complete genome sequences of 76 csfv representing three genotypes, we firstly analyzed the relative nucleotide composition, effective number of codon (enc) and synonymous codon usage in csfv genomes. the re ... | 2017 | 28416383 |
| genetic and virulence characterization of classical swine fever viruses isolated in mongolia from 2007 to 2015. | classical swine fever (csf), a highly contagious viral disease affecting domestic and wild pigs in many developing countries, is now considered endemic in mongolia, with 14 recent outbreaks in 2007, 2008, 2011, 2012, 2014, and 2015. for the first time, csf viruses isolated from these 14 outbreaks were analyzed to assess their molecular epidemiology and pathogenicity in pigs. based on the nucleotide sequences of their 5'-untranslated region, isolates were phylogenetically classified as either sub ... | 2017 | 28260187 |
| new leaves in the growing tree of pestiviruses. | pestiviruses are a group of viruses of veterinary importance infecting livestock animals like pigs, cattle, and sheep, and also wildlife animals like wild boar and different deer species. while for decades only four classical species (classical swine fever virus, bovine viral diarrhea virus types 1 and 2, border disease virus), and a few so-called atypical pestiviruses were known (e.g., giraffe virus, pronghorn virus, hobi virus), a series of novel pestiviruses was identified in the last years ( ... | 2017 | 29029724 |
| genetic characterization of african swine fever virus isolates from soft ticks at the wildlife/domestic interface in mozambique and identification of a novel genotype. | african swine fever virus (asfv) is one of the most threatening infectious diseases of pigs. there are not sufficient data to indicate the importance of the sylvatic cycle in the spread and maintenance of the disease locally and potentially, globally. to assess the capacity to maintain asf in the environment, we investigated the presence of soft tickreservoirs of asfv in gorongosa national park (gnp) and its surrounding villages. a total of 1,658 soft ticks were recovered from warthog burrows an ... | 2017 | 28921895 |
| roles of african swine fever virus structural proteins in viral infection. | african swine fever virus (asfv) is a large, double-stranded dna virus and the sole member of the asfarviridae family. asfv infects domestic pigs, wild boars, warthogs, and bush pigs, as well as soft ticks (ornithodoros erraticus), which likely act as a vector. the major target is swine monocyte-macrophage cells. the virus can cause high fever, haemorrhagic lesions, cyanosis, anorexia, and even fatalities in domestic pigs. currently, there is no vaccine and effective disease control strategies a ... | 2017 | 29978065 |
| epidemiological analyses of african swine fever in the baltic states and poland: (update september 2016-september 2017). | efsa assisted four countries in the analysis of epidemiological data on african swine fever (asf), collected until september 2017. the temporal analysis demonstrated that the average proportions of pcr and antibody-elisa positive samples from the hunted wild boar remained below 3.9 and 6.6, respectively. a peak in the asf incidence was observed 6 months after the first observed case, followed by a significant reduction of the number of cases and low levels of african swine fever virus (asfv) cir ... | 2017 | 32625356 |
| epidemiological analyses on african swine fever in the baltic countries and poland. | african swine fever virus (asfv) has been notified in the baltic countries and the eastern part of poland from the beginning of 2014 up to now. in collaboration with the asf-affected member states (ms), efsa is updating the epidemiological analysis of asf in the european union which was carried out in 2015. for this purpose, the latest epidemiological and laboratory data were analysed in order to identify the spatial-temporal pattern of the epidemic and a risk factors facilitating its spread. cu ... | 2017 | 32625438 |
| serologic survey for selected viral and bacterial swine pathogens in colombian collared peccaries ( pecari tajacu) and feral pigs ( sus scrofa). | in south america, wild populations of peccaries coexist with domestic and feral pigs, with poorly understood consequences. we captured 58 collared peccaries ( pecari tajacu) and 15 feral pigs ( sus scrofa) in locations of colombia where coexistence of these species is known. blood samples were tested for antibodies against four viral agents, classical swine fever virus (csfv), aujeszky's disease virus (adv), porcine circovirus (pcv-2), and vesicular stomatitis virus (new jersey and indiana subty ... | 2018 | 29902129 |
| infection of pigs with african swine fever virus via ingestion of stable flies (stomoxys calcitrans). | within eastern europe, african swine fever virus (asfv) has unexpectedly spread to farms with high biosecurity. in an attempt to explain this process, pigs were allowed to ingest flies that had fed on asfv-spiked blood, which had a realistic titre for an infected pig. some of the pigs became infected with the virus. thus, ingestion of blood-sucking flies, having fed on asfv-infected wild boar before entering stables, represents a potential route for disease transmission. | 2018 | 29877056 |
| revisiting the genetic diversity of classical swine fever virus: a proposal for new genotyping and subgenotyping schemes of classification. | classical swine fever (csf) is a highly contagious febrile viral disease caused by csf virus (csfv), and it is considered one of the most important infectious diseases that affect domestic pigs and wild boar. previous molecular epidemiology studies have revealed that the diversity of csfv comprises three main genotypes and different subgenotypes defined using a reliable cut-off to accurately classify csfv at genotype and subgenotype levels. however, a growing number of csfv both complete genome ... | 2018 | 29799671 |
| evolution in europe of african swine fever genotype ii viruses from highly to moderately virulent. | since its arrival in the caucasus and russia in 2007, african swine fever virus (asfv) has spread widely and has now affected the eu countries of estonia, latvia, lithuania, poland and, more recently, the czech republic and romania. the ever-increasing evidence of seropositive wild boar in certain areas suggests that some animals may be surviving for some time or could even be recovering from the disease. this could be due to acquired immunity after the primary infection and/or the presence of r ... | 2018 | 29778207 |
| classical swine fever in india: current status and future perspective. | classical swine fever (csf) is a globally significant disease of swine caused by classical swine fever virus. the virus affects the wild boars and pigs of all age groups, leading to acute, chronic, late-onset or in-apparent course of the disease. the disease causes great economic loss to the piggery industry due to mortality, stunted growth, poor reproductive performance, and by impeding the international trade of pig and pig products. in india, csf outbreaks are reported from most of the states ... | 2018 | 29725815 |
| efficient inhibition of african swine fever virus replication by crispr/cas9 targeting of the viral p30 gene (cp204l). | african swine fever is a devastating viral disease of domestic and wild pigs against which no vaccine or therapy is available. therefore, we applied the crispr (clustered regularly interspaced short palindromic repeats) - cas9 nuclease system to target the double-stranded dna genome of african swine fever virus (asfv). to this end, a permissive wild boar lung (wsl) cell line was modified by stable transfection with a plasmid encoding cas9 and a guide rna targeting codons 71 to 78 of the phosphop ... | 2018 | 29362418 |
| ictv virus taxonomy profile: asfarviridae. | the family asfarviridae includes the single species african swine fever virus, isolates of which have linear dsdna genomes of 170-194 kbp. virions have an internal core, an internal lipid membrane, an icosahedral capsid and an outer lipid envelope. infection of domestic pigs and wild boar results in an acute haemorrhagic fever with transmission by contact or ingestion, or by ticks of the genus ornithodoros. indigenous pigs act as reservoirs in africa, where infection is endemic, and from where i ... | 2018 | 29565243 |
| african swine fever virus biology and vaccine approaches. | african swine fever (asf) is an acute and often fatal disease affecting domestic pigs and wild boar, with severe economic consequences for affected countries. asf is endemic in sub-saharan africa and the island of sardinia, italy. since 2007, the virus emerged in the republic of georgia, and since then spread throughout the caucasus region and russia. outbreaks have also been reported in belarus, ukraine, lithuania, latvia, estonia, romania, moldova, czech republic, and poland, threatening neigh ... | 2018 | 29551143 |
| frequent infection of wild boar with atypical porcine pestivirus (appv). | the recently identified atypical porcine pestivirus (appv) was demonstrated to be the causative agent of the neurological disorder "congenital tremor" in newborn piglets. despite its relevance and wide distribution in domestic pigs, so far nothing is known about the situation in wild boar, representing an important wild animal reservoir for the related classical swine fever virus. in this study, 456 wild boar serum samples obtained from northern germany were investigated for the presence of appv ... | 2018 | 29527814 |
| modulation of type i interferon signaling by african swine fever virus (asfv) of different virulence l60 and nhv in macrophage host cells. | asfv causes an important disease of domestic swine and wild boar. currently no vaccine is available, highlighting the necessity to understand asfv modulation of innate immune responses in natural host cells. with this aim, macrophage cultures enriched in swc9 and cd163 differentiation markers were infected in parallel with high virulent asfv/l60 and low virulent asfv/nhv, the latter lacking mgf 360 and 505/530 genes associated with type i interferon (ifn i) control. ifn i production and signalin ... | 2018 | 29519508 |
| a review of african swine fever and the potential for introduction into the united states and the possibility of subsequent establishment in feral swine and native ticks. | african swine fever (asf) is caused by african swine fever virus (asfv), which can cause substantial morbidity and mortality events in swine. the virus can be transmittedviadirect and indirect contacts with infected swine, their products, or competent vector species, especiallyornithodorosticks. africa and much of eastern europe are endemic for asf; a viral introduction to countries that are currently asf free could have severe economic consequences due to the loss of production from infected an ... | 2018 | 29468165 |
| short time window for transmissibility of african swine fever virus from a contaminated environment. | since the introduction of african swine fever virus (asfv) into the baltic states and poland in 2014, the disease has continued to spread within these regions. in 2017, the virus spread further west and the first cases of disease were reported in the czech republic and romania, in wild boar and domestic pigs, respectively. to control further spread, knowledge of different modes of transmission, including indirect transmission via a contaminated environment, is crucial. up until now, such an indi ... | 2018 | 29457702 |
| complete genome sequence of an african swine fever virus (asfv pol/2015/podlaskie) determined directly from pig erythrocyte-associated nucleic acid. | african swine fever (asf) is an important disease of domestic pigs and wild boar. the disease is caused by african swine fever virus (asfv). in 2014, asfv was introduced into eastern europe, and it has since then continued to spread within various eastern european countries. investigating differences in sequences between asfv isolates may be a valuable tool to understand differences in virulence among them, however currently, no complete genome sequences of the viruses responsible for the easter ... | 2018 | 30063908 |
| pulmonary intravascular macrophages regulate the pathogenetic mechanisms of pulmonary lesions during acute courses of classical swine fever. | classical swine fever (csf) is a highly contagious and often fatal viral disease of domestic pigs and wild boar. pulmonary oedema and haemorrhages in lung parenchyma are common lesions in the acute forms of csf that may compromise pig survival and whose pathogenetic mechanisms remain unclear. the appearance of pulmonary lesions in pigs infected with alfort/187 strain of classical swine fever virus (csfv) euthanized between 2 and 17 days postinfection (dpi) and the role played by cytokines secret ... | 2018 | 30047251 |
| african swine fever: an unprecedented disaster and challenge to china. | african swine fever (asf), caused by african swine fever virus, is a hemorrhagic and often fatal disease of domestic pigs and wild boar, which is notifiable to the world organization for animal health. on august 3, 2018, china reported the first outbreak of asf in shenyang, a northeastern city of china. as of october 8, a total of 33 asf outbreaks were reported in eight provinces in china, the biggest pork producer and consumer in the world. | 2018 | 30367672 |
| inhibition of cgas-sting-tbk1 signaling pathway by dp96r of asfv china 2018/1. | african swine fever virus (asfv) is a highly pathogenic large dna virus that causes african swine fever (asf) in domestic pigs and european wild boars with mortality rate up to 100%. the dp96r gene of asfv encodes one of the viral virulence factors, yet its action mechanism remains unknown. in this study, we report that dp96r of asfv china 2018/1 strain subverts type i ifn production in cgas sensing pathway. dp96r inhibited the cgas/sting, and tbk1 but not irf3-5d mediated ifn-β and isre promote ... | 2018 | 30348523 |
| evolutionary dynamics of classical swine fever virus in south korea: 1987-2017. | the 5' utr (n=102) and full-length e2 (n=37) genes of classical swine fever viruses (csfvs) circulating in south korea over the past 30 years (1987-2017) were examined to determine the evolutionary rate and estimated time of the most recent common ancestor (tmrca). from 2000, the korean classical swine fever (csf) antigen changed from genotype 3 to 2, which comprises subgenotypes 2.1b (2002-2013) and 2.1d (2011-2017). there are genotypic variations in the full-length e2 gene of korean csfv genot ... | 2018 | 30322538 |
| the intracellular proteome of african swine fever virus. | african swine fever (asf) is a viral disease that affects members of the suidae family such as african bush pigs, warthogs, but also domestic pigs, and wild boar. it is transmitted by direct contact of naïve with infected animals, by soft ticks of the ornithodoros genus, or indirectly by movement of infected animals, improper disposal of contaminated animal products or other sources related to human activity. the recent spread of asf into eastern and central european countries is currently threa ... | 2018 | 30279544 |
| the risk of infection by african swine fever virus in european swine through boar movement and legal trade of pigs and pig meat. | african swine fever (asf) is currently spreading westwards throughout europe and eastwards into china, with cases occurring in both wild boar and domestic pigs. a generic risk assessment framework is used to determine the probability of first infection with asf virus (asfv) at a fine spatial scale across european union member states. the framework aims to assist risk managers across europe with their asf surveillance and intervention activities. performing the risk assessment at a fine spatial s ... | 2019 | 31998765 |