Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| lack of regression in epstein-barr virus infected leucocyte cultures of nasopharyngeal carcinoma patients. | 1981 | 6118752 | |
| presence of epstein-barr virus dna in tonsillar tissues. | sera from 48 tonsillar carcinoma (tc) patients, 48 matched controls and 16 recurrent exudative tonsillitis (ret) patients were examined for the presence of epstein-barr virus (ebv) associated nuclear antigen (ebna), early antigen (ea) and virus capsid antigen (vca). higher prevalence and significantly higher antibody titres against all three ebv-associated antigens were observed in tc patients in comparison with controls and ret patients. patients suffering from anaplastic tc had higher titres o ... | 1981 | 6120637 |
| physiologic concentrations of normal human plasma lipoproteins inhibit the immortalization of peripheral b lymphocytes by the epstein-barr virus. | epstein-barr virus (ebv)-induced immortalization of adult human b lymphocytes is suppressed by physiologic concentrations of human plasma lipoproteins. several inhibitory mechanisms appear to be operative. first, low density lipoproteins (ldl) directly reduce the ability of ebv to transform human b cells. second, ldl as well as intermediate and very low density lipoproteins modulate early inductive events rendering the b cell refractory to transforming signals from ebv. third, ldl also selective ... | 1981 | 6267100 |
| induction of complement receptor expression in cell lines derived from human undifferentiated lymphomas. ii. characterization of the induced complement receptors and demonstration of the simultaneous induction of ebv receptor. | we have studied the specificity of complement receptors induced by theophylline in 2 cell lines derived from undifferentiated lymphomas, one of burkitt's type, and compared it to that of complement receptors in other cell types. both c3b and c3d receptors were induced. the induced c3b receptor differed from the c3b receptor of mature normal lymphocytes, polymorphonuclear leukocytes and the cells of a nodular lymphoma in 2 respects. firstly, it bound c3b much less avidly (by a factor of several h ... | 1981 | 6267130 |
| induction of epstein-barr virus early antigen, virus capsid antigen and virus dna synthesis by mitomycin c. | the effect of mitomycin c was tested on epstein-barr virus (ebv) antigen and virus dna synthesis in producer and non-producer cell lines. we found that producer, but not non-producer cell lines, are inducible for early antigen and virus capsid antigen synthesis. although host dna synthesis is effectively inhibited by over 99% in the presence of mitomycin c, virus dna synthesis in the producer lines is unaffected and induced in parallel with the virus antigens. | 1981 | 6267175 |
| the epstein-barr virus (ebv) in the pathogenesis of posttransplant lymphoma. | 1981 | 6267753 | |
| early dna-binding polypeptides of epstein-barr virus. | 1981 | 6267784 | |
| protein kinase activity associated with epstein-barr virus-determined nuclear antigen. | 1981 | 6267796 | |
| leukocyte response in childhood infectious mononucleosis. caused by epstein-barr virus. | the usual leukocyte response in infectious mononucleosis (im) has been established in adults but not in children. previously, the diagnosis of im often was difficult in childhood due to the lack of detectable heterophil antibody response in many patients. using epstein-barr virus-specific serology, we have documented im in 45 patients between 1 and 15 years of age. the mean and ranges for the total leukocytes, percent lymphocytes, and percent atypical lymphocytes are presented. these do not vary ... | 1981 | 6267933 |
| viral infections in pediatric burn patients. | bacterial and fungal infections are frequent complications of burns, but the frequency of viral infections has not been defined. in a retrospective survey of serum for viral antibodies in pediatric burn patients, 22% had fourfold increases in antibodies to cytomegalovirus (cmv); 8% had increases to herpes simplex virus and to epstein-barr virus; and 5% had increases to varicella-zoster virus. none of the patients had evidence of adenovirus or hepatitis b virus infection. on the basis of these ob ... | 1981 | 6267934 |
| parainfectious acute obstructive hydrocephalus. | 1981 | 6267983 | |
| fatal epstein-barr virus infection in a 63-year-old man. an autopsy report. | a 63-year-old man with acute, heterophil-negative epstein-barr (ebv) viral infection displayed neurologic impairment that progressed to coma and death. fever, pharyngitis, and lymphadenopathy were notably absent. there was no lymphocytosis, and multiple peripheral smears revealed few atypical lymphocytes. results of specific ebv serology were diagnostic of acute infection. at the time of autopsy, there was massive intravascular and perivascular infiltration of all organs by lymphocytes and atypi ... | 1981 | 6268012 |
| coronavirus-associated antibodies in nasopharyngeal carcinoma and infectious mononucleosis. | coronavirus-like particles are found within the cytoplasm of npc tumor cells, within the cytoplasm of the tumor cells of the regional metastases, and within tumor cells grown on nude mice. for the immunologic identification of the coronaviruses, the cultures of human tracheal epithelium (mrc-c) were used and inoculated with a known coronavirus strain. whereas blood sera from npc patients (n = 73) contain significantly elevated antibody titers against corona viruses, unselected sera from patients ... | 1981 | 6268032 |
| synthesis of hypomethylated epstein-barr viral dna is stimulated by dimethylsulfoxide treatment of lymphoblastoid cells. | 1981 | 6268064 | |
| chromosomes and cell surface markers of marmoset lymphocytes and epstein-barr virus-transformed marmoset cell lines. | the g-banded karyotypes of both normal lymphocytes and epstein-barr virus (ebv)-transformed lymphocytes of cotton-topped marmosets (saguinus oedipus) were examined. the marmoset lymphocytes and ebv-transformed lymphoblastoid cells had normal diploid chromosomes (2n = 46) with no specific cytogenic change associated with transformation in vitro. ebv-transformed marmoset lymphocytes expressed the cell surface markers of b lymphocytes and eb viral antigens. | 1981 | 6268289 |
| variant translocation in burkitt's lymphoma: 8;22 translocation in a french patient with an epstein-barr virus-associated tumor. | a variant translocation t(8;22)(q23;q11) has been found in tumor cells from a 19-year-old white man with an epstein-barr virus-associated burkitt's lymphoma (bl). the tumor, which appeared 2 years after the patient had infectious mononucleosis, bore histopathological features characteristic of bl, although only the lymph nodes in the cervical region were involved. this case and some other recent cytogenetic observations of nonendemic bl emphasize the importance of chromosome #8 rearrangement in ... | 1981 | 6268290 |
| cytotoxic cells induced in vitro by epstein-barr virus antigens. | 1981 | 6268308 | |
| 12-o- tetradecanoylphorbol- 13 acetate (tpa) treatment elevates the natural killer (nk) sensitivity of certain human lymphoid lines. | 1981 | 6268309 | |
| [dynamics of epstein-barr virus antibodies in hodgkin's disease]. | 1981 | 6268310 | |
| dehydroepiandrosterone and 16 alpha-bromo-epiandrosterone: inhibitors of epstein-barr virus-induced transformation of human lymphocytes. | dehydroepiandrosterone (dhea), a major adrenal secretory product in men and women, is a potent inhibitor of mammalian glucose-6-phosphate dehydrogenase (g6pdh). long-term treatment with this steroid has previously been found to suppress spontaneous breast cancer development in c3h mice. dhea is now shown to inhibit epstein-barr virus (ebv)-induced morphologic transformation and stimulation of dna synthesis in human lymphocytes. 16 alpha-br-epiandrosterone, a dhea analog that is about 60 times as ... | 1981 | 6268330 |
| b lymphocytopenia in infectious mononucleosis. | changes in lymphocyte surface markers during epstein-barr virus (ebv) associated infectious mononucleosis (im) were re-examined because (1) these changes may provide information about host immune restraints to the initiation of a malignant process and (2) problems in the identification of b lymphocytes were often not appreciated in previous observations. an acute and transient reduction of igm- and igd-bearing b lymphocytes in the peripheral blood of these patients was demonstrated. the numbers ... | 1981 | 6268336 |
| differentiation of members of the human herpesviridae family by radioimmunoassay. | many individuals who are seronegative for one member of the human herpesviridae family are strongly seropositive for other members. using sera from such individuals, the radioimmunoassay technique demonstrated absence of antigen-antibody cross-reactions between varicella-zoster virus (vzv) and herpes simplex virus (hsv) at levels of less than one part in 1,000. sera containing antibody to both hsv and vzv were absorbed with antigens of one agent without significantly altering the amount of remai ... | 1981 | 6268545 |
| antibodies to epstein-barr virus-specific antigens in patients with hairy-cell leukemia. | antibody titers to epstein-barr virus (ebv)-specific antigens of 19 patients with hairy-cell leukemia (hcl) were markedly elevated. all patients showed high titers of igg anti-viral capsid antigen (vca) reactivity equal to or greater than 320 (reciprocal titer). the anti-vca reciprocal geometric mean titer (gtm) was 1106 in contrast to a gmt of 80 for healthy controls (p less than 0.001). no igm anti-vca antibody was detected, but three patients had iga anti-vca antibodies. fourteen patients had ... | 1981 | 6268553 |
| generation in vitro of ebv-induced specific cytotoxic t cells in autologous serum avoids complications due to self-preferred foetal calf serum-specific t-cell cytotoxicity. | a previous report has established that in cultures of human mononuclear leukocytes, foetal calf serum (fcs) is capable of generating high levels of t cells preferentially cytotoxic for the autologous lymphoblastoid cell line (lcl). the present study compared the capacity of epstein-barr virus (ebv) to generate cytotoxic t cells in cultures of mononuclear cells grown in fcs in this system. five ebv-seropositive and three seronegative donors were used and cultures were harvested at 14 days. with c ... | 1981 | 6268554 |
| synthesis of thymidine kinase (tk) in epstein-barr virus-superinfected raji tk-negative cells. | epstein-barr virus (ebv) from p3hr-1 cells, but not from b95-8 cells, can induce the synthesis of thymidine kinase (tk) in tk-negative raji cells. the synthesis of tk was slightly reduced, but not inhibited, when cells were cultivated in the presence of cytosine arabinoside (ara-c). on the other hand, the synthesis of tk in ordinary raji cells was enhanced in the presence of the drug. thymidine-beta-d-arabinofuranoside (ara-t) was capable of reducing the conversion rate of thymidine to tdr nucle ... | 1981 | 6268568 |
| requirements for the establishment of high-titered human monoclonal antibodies against tetanus toxoid using the epstein-barr virus technique. | 1981 | 6268704 | |
| characteristics of epstein-barr virus activation of human b lymphocytes. | epstein-barr virus (ebv) will infect at least every third cell if exposed in vitro to an extensively purified b cell population from human peripheral blood. about 10% of such infected cells will be driven into immunoglobulin synthesis and secretion, as judged by the indirect protein a plaque assay. the appearance of eb nuclear antigen, de novo dna synthesis, and immunoglobulin secretion are linked phenomena accompanying infection as judged by viral dilution experiments, which yield kinetics of a ... | 1981 | 6268732 |
| epstein-barr virus (ebv)-lymphoid cell interactions. i. quantification of ebv particles required for the membrane immunofluorescence assay and the comparative expression of ebv receptors on different human b, t and null cell lines. | we report data on the number of epstein-barr virus (ebv) particles required to detect virus binding to target cells (raji or bja-b) by means of membrane immunofluorescence (mif). after determining the optimum conditions for the mif assay the following aspects of ebv-lymphoid cell interactions were examined: (i) binding of two different strains of ebv to various types of human lymphoid cell lines, (ii) expression of receptors for both ebv and complement on these lines and (iii) induction of ebv-i ... | 1981 | 6268736 |
| epstein-barr virus membrane antigens: characterization, distribution, and strain differences. | the epstein-barr virus (ebv)-associated membrane antigen polypeptides (350,000, 220,000, 140,000, and 85,000 daltons) are recognized by a rabbit anti-ebv serum and are present on the plasma membranes of producer cell lines, as we demonstrated previously. in this report, we show that these polypeptides are present on intact virus particles. subcellular fractionation revealed that these antigens are distributed throughout the cell, except for the 85,000-dalton protein, which was poorly represented ... | 1981 | 6268807 |
| epstein-barr virus polypeptides: identification of early proteins and their synthesis and glycosylation. | we have identified at least six early polypeptides induced by epstein-barr virus in cells or under conditions which are nonpermissive for epstein-barr virus dna replication ranging in molecular weight from 140,000 to 26,000. | 1981 | 6268851 |
| in utero epstein-barr virus (infectious mononucleosis) infection. | a male infant infected in utero with epstein-barr virus (ebv) demonstrated a syndrome of multiple congenital anomalies (micrognathia, cryptorchidism, central cataracts), hypotonia, thrombocytopenia, persistent monocytosis, proteinuria, and multiple areas of metaphysitis at birth. lymphocytes were epstein-barr nuclear antigen (ebna) positive (18%) and persisted in culture for three months. he had antibody to early antigen (anti-ea), igm-viral capsid (anti-vca), and ebna (anti-ebna) detectable at ... | 1981 | 6268854 |
| infectious mononucleosis and acute leukemia. | 1981 | 6268857 | |
| atypical lymphocytosis in children. | a complete blood count performed during the evaluation of a febrile or traumatized child may show the presence of atypical lymphocytosis. although atypical lymphocytes occur in several conditions, they are most closely associated with infectious mononucleosis (im). we initiated a study to determine if the presence in children of atypical lymphocytosis without the triad of splenomegaly, pharyngitis, and adenopathy is a manifestation of im. heterophil antibody (ha) and epstein-barr virus (ebv) tit ... | 1981 | 6266286 |
| effect of novobiocin on dna synthesis and structure in human lymphoblastoid cells. | 1981 | 6266406 | |
| separation and properties of molecular forms of alpha-galactosidase and alpha-n-acetylgalactosaminidase from blood lymphocytes and lymphoid cell lines transformed by epstein-barr virus. | 1981 | 6266491 | |
| epstein-barr virus co-reconstituted with sendai virus envelopes infects epstein-barr virus-receptor negative cells. | epstein-barr virus (ebv) was co-reconstituted with sendai virus envelopes. the reconstituted "hybrid' virus could bind and penetrate into ebv-receptor negative cells. using this approach, t-cell-derived human and mouse leukemia cells, human t-lymphocytes and mouse spleen cells were successfully infected as judged by the induction of ebv-determined antigens and stimulation of dna synthesis. the t-cell-derived human leukemia line molt-4, that can absorb ebv but without virus penetration, could be ... | 1981 | 6266500 |
| chromosomes and causation of human cancer and leukemia. xlv. chromosome patterns in stimulated lymphocytes of chronic lymphocytic leukemia. | in the present study the b-cell mitogens from epstein-barr virus (ebv) and lipopolysaccharide-b from e. coli (lps) were used to stimulate the blood cells of 27 patients with chronic lymphocytic leukemia (cll) to undergo mitosis. in 14 cll cases ebv was used as the only mitotic stimulant and in 5 cases both ebv and lps. in 8 cases neither ebv nor lps caused mitotic cells to appear. in 9 cases the cells were characterized by abnormal clones, including 5 cases with +12 (including 1 case with a poss ... | 1981 | 6266649 |
| combined effect of the extracts from croton tiglium, euphorbia lathyris or euphorbia tirucalli and n-butyrate on epstein-barr virus expression in human lymphoblastoid p3hr-1 and raji cells. | the combined usage of n-butyrate and 12-o-tetradecanoylphorbol-13-acetate (tpa) or the oily extracts from croton tiglium, euphorbia lathyris or euphorbia tirucalli exerted a marked effect on induction of epstein-barr virus (ebv)-associated early (ea) and viral capsid (vca) antigens in ebv genome-carrying human lymphoblastoid cell lines. in producer p3hr-1 cells, the enhancing effect of the 2 components was additive both for ea and vca, while in non-producer raji cells, a synergistic increase of ... | 1981 | 6266651 |
| [levels of epstein-barr virus antibodies in the serum of children with acute lymphoblastic leukemia]. | 1981 | 6266677 | |
| detection of anti-platelet antibodies in patients with idiopathic thrombocytopenic purpura (itp) and in patients with rubella and herpes group viral infections. | a highly sensitive enzyme-linked immunosorbent assay (elisa) technique was observed for serological detection of antibodies against platelets. a covalently bound to sepharose cl-4b was used to enrich sera in the igg3 subclass of antibodies. the elisa procedure as applied to detect anti-platelet antibodies in patients with herpes or rubella viral infections and in patients with idiopathic thrombocytopenic purpura (itp). twenty-eight sera from 13 thrombocytopenic patients showed high levels of ant ... | 1981 | 6266723 |
| fc and c3 receptor patterns on two ebv- negative burkitt lymphoma lines during acute exposure to ebv (p3hr-1 substrain). | 1981 | 6266834 | |
| restriction of foetal calf serum-induced cytotoxicity by human t-cells. | 1981 | 6266949 | |
| an immunological approach to the infectious mononucleosis syndrome. | 1981 | 6264664 | |
| ultraviolet inactivation of epstein--barr virus-induced nuclear antigen (ebna), dna and igm synthesis in human b lymphocytes. | 1981 | 6264682 | |
| the chinese epidemiological approach of nasopharyngeal carcinoma research and control. | nasopharyngeal carcinoma (npc), the major cancer killer in cantonese chinese, represents one of the three cancer research priorities in china. apart from some fundamental research projects on the epstein-barr virus (ebv), their main efforts are epidemiologically and clinically oriented. major surveys in guangdong and guangxi provinces showed that high ebv/iga/vca serum levels can detect npc at an early stage of the disease. characterization of "precancerous lesions" in the nasopharynx and among ... | 1981 | 6264696 |
| beneficial effects of human viruses. | in keeping with the theme of this yale-china symposium, we discuss some unexpected dividends which have been derived from the basic study of five viruses to which man has been exposed. inquiring into the behavior of these viruses for their own sake has not only produced an increase in basic understanding of biologic processes, but has provided concepts and techniques which will broaden our knowledge of the etiology, pathogenesis, and treatment of human diseases which are unrelated to viruses. | 1981 | 6264697 |
| radiation effects on cultured human lymphoid cells. analysis using the growth extrapolation method. | the cloning efficiency of human normal and malignant lymphoid cells is usually low. radiation effects in vitro on such cells can therefore not be analysed with conventional cloning. however, this problem can be circumscribed by using the growth extrapolation method. a panel of human leukemia-lymphoma cell-lines representing epstein-barr virus carrying lymphoblastoid cells of presumed nonneoplastic derivation and neoplastic t- and b-lymphocytes was used to test the efficiency of this method. the ... | 1981 | 6264744 |
| epstein-barr virus. its relationship to the pathogenesis of rheumatoid arthritis. | 1981 | 6264928 | |
| characterization of the epstein-barr virus isolated from a cell line derived from a patient with american burkitt's lymphoma. | epstein-barr viral (ebv) dna is nearly always detectable in african burkitt's lymphoma (bl) but is infrequently found in the histologically indistinguishable american bl. we have derived a tumor cell line from a patient with american bl which produces ebv, and we have compared this virus isolate [jlp(c)] with african bl ebv. the american jlp(c) virus immortalizes human umbilical cord lymphocytes in vitro, and its dna is indistinguishable from african bl ebv dna by nucleic acid hybridization and ... | 1981 | 6265076 |
| examination of epstein-barr virus and c-type proviral sequences in american and african lymphomas and derivative cell lines. | while epstein-barr viral (ebv) dna is nearly always detectable in african burkitt's lymphoma (bl), the relatively low frequency of bl in ebv-positive african children and the infrequent finding of ebv in american bl suggest that other cofactors may contribute to the malignant transformation. among these possible cofactors are type c oncornaviruses. to evaluate this possibility, we screened the cellular dna from 16 lymphomas (2 african, 14 american) and the dna from 20 lymphoma-derived cell lines ... | 1981 | 6265077 |
| age-related restriction of the light chain heterogeneity of anti-igg antibodies induced by epstein-barr virus stimulation of human lymphocytes in vitro. | 1981 | 6265130 | |
| epstein-barr virus in nontumorigenic and tumorigenic nasopharyngeal carcinoma (npc) somatic cell hybrids. | somatic cell hybrids between mouse fibroblasts and human cells derived from nasopharyngeal carcinoma (npc) biopsies or npc tumors propagated in nude mice were examined for the expression of the epstein-barr nuclear antigen (ebna), retention of epstein-barr viral (ebv) dna, and tumorigenicity in nude mice. in all hybrids the expression of ebna correlated with the detection of ebv-dna. after more than 2 years in culture, the hybrids examined retained similar amounts of ebv-dna when compared to pre ... | 1981 | 6265479 |
| nadh cytochrome b5 reductase activity in lymphoid cell lines. expression of the defect in epstein barr virus transformed lymphoblastoid cell lines from patients with recessive congenital methemoglobinemia. | recessive congenital methemoglobinemia (rcm) is due to the homozygous deficiency of nadh-cytochrome b5 reductase (ec 1.6.2.2.). in type i disease, in which the patients are only methemoglobinemic, the enzyme defect is fully expressed in the erythrocytes, whereas the leukocytes are much less affected. in type ii disease, in which the patients are, in addition, mentally retarded, the defect is generalized to all the tissues including cultured fibroblasts. in the present study we have investigated ... | 1981 | 6265499 |
| discordant expression of 2 epstein-barr virus-associated antigens, ebna and rana, in man-rodent somatic cell hybrids. | the epstein barr nuclear antigen (ebna) and the rheumatoid arthritis nuclear antigen (rana) develop in human b lymphocytes that have been infected and transformed by epstein barr virus (ebv). antibodies to rana and ebna are found only in individuals with prior exposure to ebv. the purpose of the present studies was to determine the relation of the 2 antigens to each other and to ebv genetic material, in human-rodent somatic cell hybrids. cultured human b lymphoblastoid cells, raji, daudi, and rp ... | 1981 | 6265553 |
| stimulation of immunoglobulin biosynthesis in human b cells by wheat germ agglutinin. i. evidence that wga can produce both a positive and negative signal for activation of human lymphocytes. | wheat germ agglutinin (wga), previously regarded strictly as a nonmitogenic or anti-mitogenic lectin, can under appropriate conditions markedly stimulate in vitro synthesis and secretion of immunoglobulin (ig) by human b lymphocytes. stimulation of ig production by wga is 1) confined to a narrow lectin dose range (2 to 10 micrograms/ml; 2) abrogated by the simple sugar n-acetyl-d-glucosamine but not by a variety of other monosaccharides; 3) effective only after early additions of wga within the ... | 1981 | 6265558 |
| development of an anticomplement immunoenzyme test for detection of eb virus nuclear antigen (ebna) and antibody to ebna. | an anticomplement immunoenzyme test was developed by conjugating anti-human c3 antibody with horseradish peroxidase. ebna could be detected by this test in all cell lines related to eb virus, as well as in nasopharyngeal carcinoma cells, but not in unrelated cell lines. antibody to ebna could also be detected. the test is sensitive and does not require a fluorescence microscope which makes it particularly suitable for mass survey. | 1981 | 6265562 |
| the lymphocyte in infections with epstein-barr virus and cytomegalovirus. | 1981 | 6265567 | |
| j chain biosynthesis in pre-b cells and other possible precursor b cells. | human cell lines that resemble precursors in the b cell lineage have been found to synthesize j chain. in vivo pulse labeling, together with in vitro translation of total cellular rna in a wheat germ cell-free system, detected the synthesis of j chain in immunoglobulin-secreting cell lines, in a cell line with only surface igm, as well as in the pre-b-like cell line josh 4 and the round cell lines josh 7 and klm 2. the primary translation products of j chain from all of these cell lines were fou ... | 1981 | 6265589 |
| cerebral thrombosis, platelet aggregation and viral infection. | 1981 | 6265642 | |
| three phases in transformation of human lymphocytes by epstein-barr virus, as revealed by colony formation in soft agar. | human cord lymphocytes were examined for colony formation in soft agar medium at various times after infection with epstein-barr virus (ebv). the transformation process could be divided into the following three sequential phases: (1) a pre-early phase, lasting about 10 hr post infection (pi), during which the number of transformed colonies was virus dose dependent. (2) an early phase, between about 10 and 60 hr pi, during which cloning efficiency was markedly lower than that of the pre-early pha ... | 1981 | 6265738 |
| [clinical, laboratory and serological observations in infectious mononucleosis]. | 1981 | 6265847 | |
| [epstein-barr virus infections in childhood]. | 1981 | 6265848 | |
| [the incidence of juvenile epstein-barr virus infections]. | 1981 | 6265851 | |
| on the mechanism of selective inhibition of herpesvirus replication by (e)-5-(2-bromovinyl)-2'-deoxyuridine. | bromovinyldeoxyuridine (bvdurd) is a potent antiherpesvirus compound with low cytotoxicity. to gain an insight into its selectivity and mechanism of inhibition, we chemically synthesized the 5'-triphosphate of bvdurd, bvdutp, and tested its effect on the activities of dna polymerases [dna nucleotidyltransferase (dna directed), ec 2.7.7.7] of two herpesviruses--i.e., herpes simplex virus type 1 (hsv-1) and epstein-barr virus (ebv)--as well as cellular dna polymerases alpha, beta, and gamma. the e ... | 1981 | 6265902 |
| dissection of distinct human immunoregulatory t-cell subsets by a monoclonal antibody recognizing a cell surface antigen with wide tissue distribution. | a monoclonal antibody, pvr-11, was obtained after hybridization of x63ag8.653 murine myeloma cells with spleen cells from a mouse immunized with human lymphocytes. it recognizes a 175,000- to 185,000-dalton surface antigen present on approximately 80% of normal human peripheral t lymphocytes, 50% of non-t non-b cells, and less than 10% of b cells as determined by complement-dependent microcytotoxicity. it is also present on various leukemia t cells, on some but not all t lymphoblastoid cell line ... | 1981 | 6265938 |
| the epstein-barr virus in the pathogenesis of posttransplant lymphoproliferative disorders. clinical, pathologic, and virologic correlation. | twelve renal transplant patients with lymphoproliferative disorders (lpds) were studied. two clinical patterns were identified: (1) young patients present with an infectious mononucleosis-like illness with fever, sore throat, and lymphadenopathy soon after transplantation or antirejection therapy. many organs are ultimately involved, and the clinical course is one of a rapidly fatal lpd. (2) older patients present a longer time after transplantation with symptoms of solid tumors involving the ce ... | 1981 | 6266059 |
| integration of oncogenic viruses in mammalian cells. | 1981 | 6263817 | |
| isolation of epstein barr-virus and studies of its neutralization by human igg and complement. | an isolation procedure for epstein-barr virus (ebv) that yields substantial quantities of purified infectious virus is described. the transforming strain of ebv was obtained from the marmoset lymphoma cell line b95-8 after stimulation with the tumor promoter, 12-o-tetradecanoylphorbol-13-acetate (tpa). purification was achieved by dextran density gradient ultracentrifugation in the presence of bacitracin, which was included to prevent viral aggregation. when assayed in cord blood leukocytes, iso ... | 1981 | 6263973 |
| epstein barr virus selective t cells in infectious mononucleosis are not restricted to hla-a and b antigens. | 1981 | 6263974 | |
| pathophysiologic analysis of peripheral blood lymphocytes from patients with primary immunodeficiency. i. ig synthesis by peripheral blood lymphocytes stimulated with either pokeweed mitogen or epstein-barr virus in vitro. | in the present study 5 patients with common variable hypogammaglobulinemia (cvh) and 4 patients with selective iga deficiency (iga-d) were analyzed for the cellular defects responsible for impaired ig synthesis with use of peripheral blood lymphocytes stimulated with either pwm or ebv in vitro. by the use of co-culture with pwm, all the patients examined had intrinsic b cell defects restricted to the synthesis of ig class corresponding to the low or absent ig class(es) in the sera. two types of ... | 1981 | 6263975 |
| biologic and molecular characterization of the igg serum blocking factor (sbf-igg) isolated from sera of patients with ebv-induced infectious mononucleosis. | our laboratory has previously reported the isolation of a serum blocking factor (sbf) from infectious mononucleosis (im) patients. the sbf has been purified by a combination of sephadex qae-50 ion exchange and sephadex g-200 molecular sieve chromatography. this material was found to be devoid of soluble immune complexes, and immunochemically and biochemically was characterized as igg and, hence, termed sbf-igg. the sbf-igg was shown to significantly (alpha = 0.05) suppress antigen specific (infl ... | 1981 | 6263976 |
| plasmacytic differentiation of circulating epstein-barr virus-infected b lymphocytes during acute infectious mononucleosis. | during the acute phase (1 wk of symptoms or less) of infectious mononucleosis (im), 70--80% of circulating epstein-barr virus nuclear antigen (ebna)-positive cells have differentiated toward plasma cells. thus the characteristics of the infected cells in the majority of im patients during early disease are indistinguishable from ebna-positive tumor cells of a previously reported child who developed lymphoma during im. iga and igg were the most frequent and igm the least frequent immunoglobulin i ... | 1981 | 6264016 |
| conservation and progressive methylation of epstein-barr viral dna sequences in transformed cells. | the structure of intracellular viral dna from a number of cell lines arising by clonal transformation of human lymphocytes in vitro with epstein-barr virus was analyzed. intracellular viral dnas were partially purified and digested with several restriction endonucleases, and the products of digestion were separated by electrophoresis in agarose gels. the viral fragments were detected by transferring the dna from the gel to nitrocellulose sheets, hybridizing radiolabeled recombinant vectors carry ... | 1981 | 6264105 |
| phosphorylation of acyclovir [9-(2-hydroxyethoxymethyl)guanine] in epstein-barr virus-infected lymphoblastoid cell lines. | the extent of phosphorylation of 9-(2-hydroxyethoxymethyl)guanine (acyclovir [acv]) in fresh peripheral leukocytes, in epstein-barr virus (ebv)-infected lymphoblastoid cell lines, and in herpes simplex virus type 1-infected lymphoblastoid (p3hr-1) and monkey kidney (vero) cells was determined by high-pressure liquid chromatography, mono-, di-, and triphosphorylated derivatives of [8-14c]acv were detected at low levels at various times after superinfection of raji cells with ebv. the extent of ph ... | 1981 | 6264131 |
| epstein-barr virus dna. ix. variation among viral dnas from producer and nonproducer infected cells. | a comparative analysis of three epstein-barr virus dnas from american patients with infectious mononucleosis (b95-8, cherry, and lamont) and four epstein-barr virus dnas from african patients with burkitt lymphoma (ag876, w91, raji, and p3hr-1) indicated that the usual format of epstein-barr virus dna includes a variable number of direct repeats of a 0.35 x 10(6)-dalton sequence (tr) at both ends of the dna, a 9 x 10(6)-dalton sequence of largely unique dna (us), a variable number of repeats of ... | 1981 | 6264134 |
| sedimentation characteristics of newly synthesized epstein-barr viral dna in superinfected cells. | replicating epstein-barr virus (ebv) dna molecules isolated from superinfected raji cells were shown to consist of 80s to 65s and 58s (mature) molecules pulse-chase experiments showed that radioactive label of dnas molecules with the larger sedimentation coefficients was partially chased into 58s labeled forms. formation of large concatemers of viral dna could not be detected at any time after superinfection. the continuous presence of the 65s viral dna intermediate throughout the replicative cy ... | 1981 | 6264162 |
| fatal lymphoproliferation and acute monocytic leukemia-like disease following infectious mononucleosis in the elderly. | three elderly patients are reported, in whom serologically confirmed recent infectious mononucleosis is followed by fatal lymphoproliferation (case 1), by acute monocytic leukemia (case 2), and by acute probably monocytic leukemia (case 3). | 1981 | 6264201 |
| sera of patients with hairy cell leukemia immunoprecipitate ebv-related antigens. | 1981 | 6264236 | |
| sequential appearance of epstein-barr virus nuclear and lymphocyte-detected membrane antigens in b cell transformation. | 1981 | 6264317 | |
| immunologic detection of occult primary cancer of the head and neck. | because antibody titers against epstein-barr virus (ebv) antigens often are elevated in patients with active undifferentiated and nonkeratinizing nasopharyngeal carcinoma, anti-ebv serologic tests have been applied as a diagnostic aid in patients who have metastatic lymphadenopathy in the neck without an obvious source. in this study of 44 patients, the serologic testing procedure proved useful in identifying six patients with nasopharyngeal carcinoma that was initially occult but was eventually ... | 1981 | 6264367 |
| embryonic erythroid differentiation in the human leukemic cell line k562. | k562 human leukemia cells synthesize embryonic hemoglobins after culture in the presence of hemin. we have rigorously identified these hemoglobins by globin chain analysis and peptide mapping. no adult hemoglobin could be detected, and beta-globin synthesis was less than 2 ppm of total protein synthesis. persistent embryonic globin gene expression is known to occur as a consequence of globin gene deletions. however, restriction endonuclease mapping showed that the globin gene complexes in k562 c ... | 1981 | 6264439 |
| virology and immune mechanisms. | epstein-barr virus and hepatitis b virus are two agents which chronically infect man and have been associated with specific neoplasms. such findings point the way to new methods of control and detection of human malignancies. furthermore, immunosuppression is another major factor underlying the development of certain malignancies in human populations. it is clear that congenital immunodeficiency states as well as organ transplant recipients requiring iatrogenic immunosuppression and cancer patie ... | 1981 | 6263441 |
| genome of a mononucleosis epstein-barr virus contains dna fragments previously regarded to be unique to burkitt's lymphoma isolates. | we wished to learn whether the genomes of strains of emb isolated from patients with infectious mononucleosis are consistently distinguishable from those of strains from burkitt's lymphoma. the genome of a new transforming strains (ff41) of ebv isolated from saliva of a patient with uncomplicated infectious mononucleosis was compared with the dna of b95-8, the only other available virus from mononucleosis. it had been found previously that b95-8 has a deletion of about 8 md in the region of the ... | 1981 | 6263500 |
| cloning of epstein-barr virus (ebv) dna fragments in pbr32 and charon3a. | restriction fragments of epstein-barr virus (ebv; b95-8) dna were cloned in the tc gene of pbr322 (hindiii-f, -g, -i, -j, -k, -l, and -m) and in charon3a (ecori-gi and -g2). altogether these cloned fragments covered 39% of the entire viral genome. the cloned ecori-g2 fragment of ebv (b95-8) dna was shown to contain, in addition to hindiii-j, two more hindiii-fragments : hindiii-m, which had not been located on the linkage map of the viral genome (given and kieff, 1978) and hindiii-n, which had b ... | 1981 | 6263760 |
| infections due to epstein-barr virus during pregnancy. | 1981 | 6262414 | |
| simultaneous congenital infection with epstein-barr virus and cytomegalovirus. | 1981 | 6262641 | |
| herpesvirus-transformed cytotoxic t-cell lines. | investigations of cellular cytotoxicity of the immune system are hampered by the lack of continuously growing, transformed cell lines which express a cytotoxic potential. here we describe cytotoxic cell lines from the cotton-topped marmoset monkey, transformed by herpesvirus ateles (hva) or herpesvirus saimiri (hvs), which can kill certain target cells in a short-term in vitro test. hva/hvs-transformed cells have earlier been classified as belonging to the t-cell lineage in contrast to epstein-b ... | 1981 | 6262648 |
| two small rnas encoded by epstein-barr virus and complexed with protein are precipitated by antibodies from patients with systemic lupus erythematosus. | primate cells harboring the epstein-barr virus (ebv) genome synthesize large amounts of two small rnas:eber 1 and eber 2 (ebv-encoded rna). these rnas are approximately 180 nucleotides long, possess 5' pppa termini, and lack poly(a). they have different t1 and pancreatic rnase digestion fingerprints. they are not found in normal b lymphocytes, in transformed b lymphocytes that lack ebv dna, in t lymphocytes transformed by herpesvirus ateles, or in a variety of other nonlymphoid mammalian cells. ... | 1981 | 6262773 |
| the kissing virus. | 1981 | 6262973 | |
| induction of replication of epstein-barr virus dna by 12-o-tetradecanoyl-phorbol-13-acetate. ii. inhibition by retinoic acid and 9-(2-hydroxyethoxymethyl) guanine. | 1981 | 6263008 | |
| copy number and location of epstein-barr viral genomes in neonatal human lymphocytes transformed after separation by size and treatment with mitogens. | 1981 | 6263010 | |
| a defect in the formation of uropod-bearing lymphocytes (hand-mirror cells) in patients with the wiskott-aldrich syndrome. | the formation of a uropod by lymphocytes (hand-mirror cells) represents a morphologic stage of immune activation and motility in lymphocytes. immune complexes have been previously shown to induce hand-mirror-cell (hmc) formation in human lymphocytes and have been associated with increased number of hmc in acute leukemia. we studied the ability of immune complexes to induce hmc in patients with wiskott-aldrich syndrome (was), a disease of cellular immune deficiency. our findings indicate that lym ... | 1981 | 6263088 |
| ocular involvement in infectious mononucleosis. | 1981 | 6263094 | |
| malignant b-cell lymphoma following and associated with infectious mononucleosis. a comparison of two cases. | the present report describes two young males with clinically diagnosed infectious mononucleosis (im) who subsequently were diagnosed as having malignant b-cell lymphoma (i.e., immunoblastic sarcoma of b-cells). despite these apparent similarities, there were fundamental differences between the two cases. the first patient, who lymphoma was diagnosed 9 months after im, was one of a well-described kindred with the x-linked lymphoproliferative syndrome (xlp) in which affected young males lack the a ... | 1981 | 6263125 |
| antibodies to epstein-barr virus, herpes simplex type 1, cytomegalovirus and measles virus in psychiatric patients. | distribution of antibodies to herpes simplex type 1 (hsv1), epstein-barr virus (ebv), cytomegalovirus (cmv) and measles virus (mv) was studied in sera and cerebrospinal fluids (csf) of 41 patients with schizophrenia, 27 patients with primary affective disorders and 25 control patients with neurological diseases. no significant differences in distribution and mean geometric titers (gmt) of antibodies to hsv1 between the psychiatric and control groups were found. distribution and gmt of antibodies ... | 1981 | 6263228 |
| immunospecificity of antisera against nonhistone nuclear proteins of human lymphoid cells grown in culture. | rabbit antibodies were obtained to nonhistone protein--dna complexes (dehistonized chromatin) prepared from two human lymphoblastoid cell lines: the conception line from an american burkitt lymphoma and nc-37 from a nonmalignant source. both antisera showed a high degree of specificity for nuclear proteins of their respective cell lines. this specificity was evident in the reactivity of both whole chromatin and dehistonized chromatin using a quantitative micro-complement fixation assay. the resu ... | 1981 | 6261962 |
| very late relapses in patients with burkitt's lymphoma: clinical and serologic studies. | of 117 patients in remission for at least 12 months after chemotherapy for confirmed burkitt's lymphoma, 14 subsequently relapsed. frequency of ever relapsing in this group varied from 12% 2 years after chemotherapy to 3-8% 3-6 years after chemotherapy. risk of very late relapse (vlr) increased with the occurrence of meningeal disease and/or relapse before a remission of 12 months or more was achieved. the use of combination chemotherapy and especially prophylactic intrathecal methotrexate signi ... | 1981 | 6259398 |
| role of infection in guillain-barré syndrome: laboratory confirmation of herpesviruses in 41 cases. | serological evidence of either acute cytomegalovirus (cmv) or epstein-barr virus (ebv) infection was sought in a large series of patients with guillain-barré syndrome (gbs) and control subjects. using an indirect immunofluorescent technique, igm antibody directed against cmv was found in the serum of 33 of 220 gbs patients. the cmv-positive patients were mainly young adults (average age, 25.6 years), previously healthy, and the most common prodromal illness was mild coryza. alternations in serum ... | 1981 | 6261680 |
| biochemical characterization of epstein-barr virus membrane antigen associated glycoproteins. | 1981 | 6261758 | |
| activation of the alternative complement pathway by normal and transformed cells in homologous and heterologous sera. | a variety of both normal and viral transformed or infected cells have been tested for their ability to activate the alternative pathway of complement in either homologous or heterologous sera. normal cell lines fail to produce alternative pathway activation in homologous sera but readily activate the alternative pathway in heterologous sera. in contrast, human cells, persistently infected with measles virus or chicken cells transformed with oncogenic avian viruses, produce a marked activation in ... | 1981 | 6260511 |