Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| in vivo and in vitro models of demyelinating diseases. ii. persistence and host-regulated thermosensitivity in cells of neural derivation infected with mouse hepatitis and measles viruses. | 1978 | 211715 | |
| [inactivation of viruses in water by anodic oxidation (author's transl)]. | the inactivating effect of anodic oxidation on viruses performed in an experimental electrolytic polarization unit was investigated with 11 different viruses suspended in tap and superficial water. furthermore attempts were made to define the influence of factors important in experimental and water respect. using concentrations up to 10(4) infectious units per ml representing a multiple quantity of the virus demonstrable in contaminated environmental waters all the viruses investigated could be ... | 1978 | 211753 |
| differential inhibitory effects of 5-bromodeoxyuridine on vaccinia and monkeypox viruses. | replication of vaccinia and monkeypox viruses was impeded in the presence of 5-bromodeoxyuridine (budr) in rl-33 cells derived from rabbit lung tissue. the different degree of inhibition was found among strains of those viruses, in which it was evident that five strains (cv-1, lister, ihd, dairen-i and ikeda) of vaccinia viruses resulted in more reduced yields of infectious virus than four strains (sen-19, orang utan, copenhagen and sierra leone) of monkeypox viruses. there was also strain varia ... | 1978 | 212474 |
| ir-genes in h-2 regulate generation of anti-viral cytotoxic t cells. mapping to k or d and dominance of unresponsiveness. | h-2 dependent and virus-specific ir genes regulate the generation of primary virus-specific k or d restricted cytotoxic t-cell responses in vivo. the following examples have been analyzed in some detail: first, dk restricted responses to vaccinia in sendai viruses are at least 30 times lower than the corresponding k-restricted responses irrespective of the h-2 haplotypes (k, b, d, dxs, dxq) of k and i regions; in contrast, lcmv infection generates high responses to dk. these findings are consist ... | 1978 | 212503 |
| inverted terminal repeats in rabbit poxvirus and vaccinia virus dna. | in both rabbit poxvirus and vaccinia virus dna have demonstrated an identical distribution of eight hinfi. the length of the terminal repeats was found to be 3.4 to 3.6 megadaltons (mdaltons) for rabbit poxvirus dna and 7.4 to 8.0 mdaltons for vaccinia virus dna. maps of the hinfi restriction sites within isolated ecori end fragments of rabbit poxvirus and vaccinia virus dna phave demonstrated an identical distribution of eight hinfi sites in an internal part (approximately 2 mdaltons) of the ec ... | 1978 | 212601 |
| activation of endogenous type c virus in balb/c mouse cells by herpesvirus dna. | several virion and nonvirion dnas were tested for the ability to activate endogenous type c virus in balb/c-derived mouse cells using the calcium precipitation technique. the dnas from all herpesviruses tested activated xenotropic type c virus synthesis. these included dnas from herpes simplex virus types 1 and 2, epstein-barr virus, human cytomegalovirus, sa8 virus, infectious bovine rhinotracheitis virus, pseudorabies virus, and herpes saimiri virus (m-dna). in contrast, dnas from vaccinia vir ... | 1978 | 212761 |
| [suppression of viral reproduction by means of specific action on the cell]. | 1978 | 212895 | |
| viral antibodies in cerebrospinal fluid of multiple sclerosis and control patients: comparison between radioimmunoassay and conventional techniques. | cerebrospinal fluid antibodies to measles, rubella, vaccinia, herpes simplex, and varicella-zoster viruses in four patient study groups (clinically definite multiple sclerosis [ms], early probable ms, optic neuritis, and control patients with other neurological diseases) were assayed by radioimmunoassay, complement fixation, hemagglutination-inhibition, or complement-enhanced plaque reduction methods. antibodies were more frequently found and at higher dilutions by radioimmunoassay than by other ... | 1978 | 203605 |
| defective macrophage immunity in newborn rabbits with fibroma virus-induced tumors. | 1978 | 203693 | |
| identification and study of a poxvirus isolated from wild rodents in turkmenia. | a new poxvirus was isolated in 1974 from the kidney of a wild big gerbil (rhombomys opimus) caught in turkmenia, where these gerbils are wide-spread. the virus resembles cowpox virus and is markedly different from the virus of infectious ectromelia, the best-known poxvirus of rodents. the new virus is apparently identical to other poxvirus isolates made from white rats and felidae in the moscow zoo. experimental inoculation of the natural hosts--big gerbils and yellow susliks (citellus fulvus)-- ... | 1978 | 204271 |
| comparison of five poxvirus genomes by analysis with restriction endonucleases hindiii, bami and ecori. | 1978 | 202667 | |
| 5-o-alkylated derivatives of 5-hydroxy-2'-deoxyuridine as potential antiviral agents. anti-herpes activity of 5-propynyloxy-2'-deoxyuridine. | alkylation of 5-hydroxyuridine or 5-hydroxy-2'-deoxyuridine with various activated alkylating agents in the presence of 1 equiv of naoh gave a series of new nucleoside analogues which were evaluated for antiviral activity against vaccinia virus, herpes simplex-1 virus, and vesicular stomatitis virus in both primary rabbit kidney cells and human skin fibroblasts. one of these analogues, 5-propynyloxy-2'-deoxyuridine, was a potent inhibitor of herpes simplex virus. structure-activity consideration ... | 1978 | 202709 |
| dna-binding proteins in the cytoplasm of vaccinia virus-infected mouse l-cells. | mouse l cell fibroblasts were infected with vaccinia virus and labeled 2 to 3 h postinfection with [35s]methionine. labeled proteins were fractionated on native and denatured dna-cellulose columns and then analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. twenty-four 90,000 to 12,500, were detected. vdp-12a (molecular weight, 29,750) had affinity for denatured but not native dna, and its synthesis was dependent on viral dna replication. vdp-20 (molecular weight, 41,000) boun ... | 1978 | 202739 |
| cell-mediated immunity to measles virus in multiple sclerosis: correlation with disability. | the blastogenic transformation of lymphocytes on exposure to antigen was used to investigate cell-mediated immune responses to viruses implicated in the pathogenesis of multiple sclerosis. in 12 patients with "early" multiple sclerosis, lymphocyte reactivity to measles virus, parainfluenza virus, and vaccinia virus did not differ significantly from reactivity in a healthy control group. however, there was a significant inverse correlation between the lymphocyte blastogenic response to measles an ... | 1978 | 202889 |
| isolation of two dna-binding proteins from the intracellular replication complex of vaccinia virus. | 1978 | 203092 | |
| cell-mediated cytotoxicity against virus-infected target cells in humans. ii. interferon induction and activation of natural killer cells. | the mechanisms by which human lymphocytes lyse virus-infected allogeneic fibroblast cultures were analyzed with particular consideration of the role of anti-viral antibodies and interferon. human cells infected with viruses were able to induce high levels of interferon upon contact with human lymphocytes. interferon, whether produced by lymphocytes after direct infection with virus or induced upon exposure of lymphocytes to virus-infected fibroblasts, appeared to be responsible for enhancing the ... | 1978 | 150448 |
| t-cell populations specifically depleted of alloreactive potential cannot be induced to lyse h-2-different virus-infected target cells. | mouse lymphocyte populations of one parental h-2 type (a) were specificially depleted of alloreactive potential by filtration through irradiated a x b f1 recipients, and thoracic duct cells were then stimulated with virus in an a x b f1 environment. experiments using t cells that had previously been exposed to influenza virus in the context of a established that cross-priming for recognition of viral components expressed on h-2-different (b) target cells does not occur. furthermore, immunologica ... | 1978 | 78955 |
| [a case of vaccinia translata]. | 1978 | 79272 | |
| inductive requirements for the generation of virus-specific t lymphocytes. i. the nature of the host cell-virus interaction that triggers secondary poxvirus-specific cytotoxic t lymphocyte induction. | 1978 | 79615 | |
| studies on cotia virus--an unclassified poxvirus. | this paper is a report of studies on cotia virus; this had been first isolated in 1965 in brazil and was subsequently shown to be a poxvirus. cotia virus grew in a wide range of cell cultures and on the chick chorioallantois (cam), its growth characteristics are similar to those of other poxviruses. microscopy showed virus factories or type b inclusions appearing before infectious progeny virus could be demonstrated. type a inclusions appeared later, after development of progeny virus; these wer ... | 1978 | 80446 |
| the effect of certain mannich n-bases, derivatives of isatin beta-thiosemicarbazone, on the replication of vaccinia virus in in vitro studies. | twenty-three mannich mono- and bis-n-bases, derivatives of isatin beta-thiosemicarbazone (ibt), were synthetized. eighteen of these were tested for their inhibitory effect on the replication of vaccinia virus (ihd strain) in cultures of chick embryo fibroblasts. all the compounds studied showed antiviral activity, demonstrated by the agar-diffusion method (screening). the concentrations that reduced the number of viral plaques to 50% (prd50) and the values of the therapeutic index (th.i.) of the ... | 1978 | 80923 |
| involvement of h-2l gene products in virus-immune t-cell recognition. evidence for an h-2l-restricted t-cell response. | the h-2l locus is closely linked to h-2d and codes for antigenic specificities present on a 45,000 mol wt glycoprotein that is distinct from the molecule which bears the d region private specificity. it was found that balb/c-h-2db mice, which lack detectable cell-surface h-2l gene products, were able to generate influenza- and vaccinia-immune cytotoxic t cells which lyse d region-compatible target cells, although they have been reported to be incapable of making a similar response to ectromelia ... | 1978 | 82606 |
| effect of double infection of cowpox virus-infected cells with paramyxovirus (sendai virus) on formation of cowpox virus-specific cell surface antigen. | the formation of cowpox virus-specific cell surface antigen (cpv s-ag) was significantly enhanced by double infection with hvj (sendai virus). simultaneous double infection, superinfection with hvj and superinfection with cpv of cells persistently infected with hvj similarly enhanced the formation of cpv s-ag, while pre-infection with hvj was ineffective. to be effective, cells must be infected at a m.o.i. of greater than or equal to 1.0 and hvj gene functions had to be expressed. the hvj-infect ... | 1978 | 88002 |
| cytotoxic t-cell responses in mice infected with influenza and vaccinia viruses vary in magnitude with h-2 genotype. | secondary effector t-cell populations generated by cross-priming with heterologous influenza a viruses operate only in h-2k or h-2d compatible situations, when assayed on sv40-transformed target cells infected with a range of influenza a viruses. the h2-kb allele is associated with a total failure in the generation of influenza-immune cytotoxic t cells, though this is not seen for the primary response to vaccinia virus. in both influenza and vaccinia development of effector t cells operating at ... | 1978 | 100569 |
| in irradiation chimeras, k or d regions of the chimeric host, not of the donor lymphocytes, determine immune responsiveness of antiviral cytotoxic t cells. | the h-2 haplotype of the chimeric host determines the responder phenotype of maturing t cells. spleen cells of chimeric mice formed when (k(k) nonresponder to d(b) x k(b) responder to d(b) plus vaccinia)f(1) bone marrow cells were used to reconstitute k(b)d(b) (c57bl/6 d(b) responder) irradiated recipients generated high levels of d(b) plus vaccinia virus-specific cytotoxic t cells. the same stem cells used to reconstitute k(k)d(b) (b10.a (2r) d(b) nonresponder) irradiated recipients resulted in ... | 1978 | 100570 |
| different rules govern help for cytotoxic t cells and b cells. | 1978 | 103005 | |
| comparative studies on poxvirus strains isolated from camels. | five poxvirus strains isolated from camels in egypt, iran and the u.s.s.r. were investigated. the morphology and size of the negatively stained virions of the investigated strains were identical. the strains produced minute white pock lesions localized mainly on the infected area of the chorioallantoic membrane, without generalization of lesions or death of embryos. the ceiling temperature and the haemagglutinating activity of the different strains were different. all the strains were apathogeni ... | 1978 | 35942 |
| preparation of highly purified vaccinia virus haemagglutinin. | 1978 | 26207 | |
| temperature-sensitive mutants of vaccinia virus. i. isolation and preliminary characterization. | fifty-seven temperature-sensitive (ts) mutants of a neurotropic variant of vaccinia virus were obtained. a comparison of the effectiveness of 6 different mutagens showed that in in vivo experiments nitrosomethylurea induced 10%, nitrosoguanidine 8%, and bromodeoxyuridine 2.5% ts mutants. in vitro, at the same degree of inactivation, uv irradiation, hydroxylamine and nitrous acid induced 2.5% ts mutants. out of 49 mutants studied, 27 had a dna+ phenotype, 19 a dna+/-and 3 a dna- phenotype. under ... | 1978 | 26208 |
| capacity of clones of vaccinia virus to multiply at different temperatures and ph values. | the study of the biological characteristics of ten clones, isolated from plaque, of vaccinia virus has made evident that two of these characteristics--the capacity to multiply at 40 degrees c and the type of cpe in animal cell cultures--can be used as genetic markers of neuropathogenicity, valued in relationship to the property to induce demyelinating encephalitis in mice injected intracerebrally or intravenously. the most neurovirulent clones demonstrated high capacity to multiply at 40 degrees ... | 1978 | 26362 |
| antiviral activity of dipyridamole derivatives. | among 46 novel pyrimido 5.4-d pyrimidine derivatives, 26 compounds were found to exhibit antiviral activity as revealed in a test programme against mengo, coxsackie b1, fowl plague, vaccinia and pseudorabies viruses, as concerns inhibition of plaque formation and of infectious virus yield. attempts to disclose structure-activity relationships by discriminant analysis pointed to a possible importance of hydrophobic substitution for the antiviral effectiveness against mengo virus of the derivative ... | 1978 | 29467 |
| 5-propyl-2'-deoxyuridine: a specific anti-herpes agent. | in both primary rabbit kidney cells and human skin fibroblasts, 5-propyl-2'-deoxyuridine proved inhibitory to herpes simplex virus at a concentration as low as 1 micrograms/ml, whereas concentrations higher than 200 micrograms/ml were required to inhibit vaccinia virus replication or normal cell metabolism. | 1978 | 233730 |
| host-determined t cell fine specificity for self-h-2 in radiation bone-marrow chimeras of standard c57bl/6 (h-2(b)) mutanthz1 (h-2 (ba)), and f (1) mice. | reciprocal radiation bone-marrow chimeras were produced between the standard c57bl/6 (=b6) and the mutant b6.c-h-2 ( ba ) (=hz1) strain. when infected with vaccinia virus, these chimeras, as well as an (hz1 × b6)=→ hz1 chimera, produced cytotoxic cells that killed vaccinia-infected h-2k(k)h-2d(b) target cells but failed to kill virus-infected h-2k(b)h-2d(d) cells. virus-infected (hz1 × b6)f(1) → b6 chimeras, however, killed both types of target. these experiments demonstrate strict t-cell specif ... | 1978 | 21302059 |
| evidence for an alkaline protease in vaccinia virus. | 1979 | 35880 | |
| antiviral activity of certain isatinisothiosemicarbazones against mengo and vaccinia virus infections in mice. | among eleven isatinisothiosemicarbazones (iits) examined only 1-ethylisatin-s-n-butylisothiosemicarbazone (zimet 189/69) proved to be significantly effective against lethal mengo and neurovaccinia virus-induced encephalitis in mice when administered subcuaneously (s.c.) in doses of 1 mmole/kg body weight per day. with peroral (p.o.) administration all the drugs failed to prevent mortality in mengo virus-infected mice. against this virus zimet 189/69 was effective over a concentration range from ... | 1979 | 35958 |
| antiviral activity of benzothiazole and benzothiazolinethione derivatives in cell cultures. | the virus inhibitory activity of benzothiazole, benzothiazolinethione and naphthothiazole derivatives was tested with vaccinia virus, newcastle disease virus (ndv) and western equine encephalomyelitis (wee) virus in the agar-diffusion plaque-inhibition test. among 58 compounds examined, 5 showed medium activity and selectivity with vaccinia virus. the highest selective effect was found with 3-(2-ethylthio-6-benzothiazolylaminomethyl)-2-benzothiazolinethione. a much lower inhibitory effect was ob ... | 1979 | 41432 |
| [participation of vaccinia virus in the pathogenesis of different clinical forms of postvaccinal complications. i. frequency of vaccinia virus detection in the vaccinted who have usual and complicated reactions to vaccination]. | the virological examination of 1365 samples taken from 469 children vaccinated against smallpox revealed considerable differences in the frequency and the time of vaccinia virus detection in different clinical forms of postvaccinal pathology as compared with uncomplicated vaccinal process. during the postvaccinal period taking its normal course vaccinia virus was isolated from 7.3% of children only from the pharynx till day 8 following vaccination. in generalized and creeping vaccinia the virus ... | 1979 | 42243 |
| variola virus strains of 1960--1975: the range of intraspecies variability and relationship between properties and geographic origin. | a comparative study of 61 variola virus strains recovered from patients in various geographic regions of the world revealed significant intraspecies variability in some biological properties, established the range of this variability and demonstrated the existence of strains with atypical properties. there was a certain correlation between some laboratory markers of variola virus and the degree of its pathogenicity for man. the prevalence of more virulent and thermoresistant strains in the hindu ... | 1979 | 42294 |
| antiviral effect of 3-[bis-(2-hydroxyethyl)-amino]-acetophenone-[4,5-diphenyl-oxyazolyl-(2)]hydrazone (imet 98/69) in mice. | the potential in vivo antiviral activity of 3-[bis-(2-hydroxyethyl)-amino]-acetophenone-[4,5-diphenyl-oxazolyl-(2)]-hydrazone (imet 98/69) was evaluated on model infections in mice. animals treated subcutaneously (s.c.) with 1 mmole of the drug per kg body weight once daily for five days were significantly protected against a lethal infection with cardioviruses, semliki forest virus and vaccinia virus. in influenza a and b virus models no antiviral activity was observed either after s.c. or oral ... | 1979 | 44666 |
| enhancement of resistance to murine osteogenic sarcoma in vivo by an extract of brucella abortus (bru-pel): association with activation of reticuloendothelial system macrophages. | the administration of an aqueous-ether extracted residue of brucella abortus (bru-pel) inhibits development of transplanted osteogenic sarcomas in mice as evidenced by a decrease in mortality. at least one mechanism through which bru-pel modulates host resistance is activation of macrophages of the reticuloendothelial system. peritoneal macrophages harvested from mice receiving bru-pel were cytotoxic for osteogenic sarcoma cells in vitro, limited the replication of vaccinia virus in cell culture ... | 1979 | 106004 |
| vaccinia virus replication. i. requirement for the host-cell nucleus. | using cytochalasin b-induced enucleation techniques, we examined the ability of vaccinia virus to replicate in the absence of the host-cell nucleus in several mammalian cell lines. it was found that virus-infected enucleated cells (cytoplasts) prepared from bsc-40, cvc, and l cells were incapable of producing infectious progeny virus. the nature of this apparent nuclear involvement was studied in detail in bsc-40 cells. modulations designed to maximize cytoplast integrity and longevity, such as ... | 1979 | 107327 |
| antiviral effect of interferon in vivo may be mediated by the host. | 1979 | 107458 | |
| [possibility of regulating the reparative activity of the cells of higher organisms by using biological factors]. | 1979 | 113952 | |
| [the effect of various suture materials on wound healing. an experimental study in animals (author's transl)]. | the reactions of connective tissue and fibers to silk, catgut, chromic catgut and polyglucolic acid fibers buried in the subcutis and to which a solution of vaccinia (who elstree strain) or a control solution was added, were tested in 7 rabbits. of the fibers tested, the histological picture on the 9th postoperative day showed that polygluconic acid fibers were by far the best, catgut fibers were particularly poor. | 1979 | 114802 |
| reciprocal stimulation of negatively selected high-responder and low-responder t cells in virus-infected recipients. | after depletion of alloreactive potential, immunologically naive t cells from c57bl/6j (kb-db) mice (b6) can be induced to respond to vaccinia virus in the context of both h-2kk and h-2db when stimulated in b10.a(4r) (kk-db) recipients. however, negatively selected b10.a(2r) (kk-db) t cells respond to h-2db-vaccinia virus but not to h-2kb-vaccinia virus when primed in an irradiated b6 environment. the b6 mouse strain is a high responder to vaccinia virus associated with h-2db, whereas the b10.a( ... | 1979 | 115003 |
| patterns of virus-immune t-cell responsiveness. comparison of (h-2k x h-2b) leads to h-2b radiation chimeras and negatively selected h-2b lymphocytes. | negatively selected h-2k(b)d(b) tdl can be induced to respond strongly to vaccinia virus presented in the context of both h-2k(k) and h-2d(b) when stimulated in irradiated h-2k(k)d(b) recipients. addition of excess (h- 2k(k)d(b) x h-2k(b)d(b))f1 tdl, which are low responders to h-2d(b)-vaccinia virus, does not obviously suppress the reactivity pattern of the h-2k(b)d(b) t cells. however, lymphocytes from chimeras made by reconstituting h- 2k(b)d(b) mice with (h-2k(k)d(k) x h-2k(b)d(b))f(l) bone ... | 1979 | 115959 |
| [microbial emission, immission and changes in the germ count in the cooling water during operation of wet cooling towers i. communication: introduction to the problem posed (author's transl)]. | 1979 | 116444 | |
| immune globulins. | 1979 | 117551 | |
| [detection and localization of a ribonuclease activity associated with vaccinia virus]. | a ribonuclease associated with vaccinia virus can be detected when reduced concentrations of nucleotides are used for an in vitro rna synthesis assay. the non-viral origin of this ribonuclease may be inferred from its external location and from its variable activity on different purified virus stocks. the detection of this ribonuclease activity on purified virus grown without foetal calf serum may suggest that this enzyme is of cellular origin. | 1979 | 120781 |
| two components specifically responsible for hemagglutination in vaccinia and cowpox viruses. | 1979 | 88801 | |
| [studies on human immunoglobulin preparations: i. viral antibody profiles (author's transl)]. | a study was undertaken to assess the viral antibody spectrum in human immunoglobulin preparations from four different manufacturers for intravenous and/or intramuscular application. studies of the kind seem to be reasonable for calculating the expected therapeutic effect of the administered immunoglobulin. the investigated 16 gammaglobulin batches showed almost the same variety of viral antibodies and only slightly differring antibody titers. it is interesting to note, that antibodies to vaccini ... | 1979 | 89620 |
| adenine arabinoside monophosphate in experimental vaccinia virus infections. | 1979 | 93408 | |
| mechanisms in the pathogenesis of post-infectious vaccinia virus encephalomyelitis in the mouse. | after intracranial replication of a neurotropic strain of vaccinia in mouse brain, analysis of the purified virus preparation reveals the presence of at least one host protein on the virus which was identified as the myelin basic protein. vaccinia virus elstree, a dermotropic virus may substitute for complete freund's adjuvant (cfa) in inducing experimental allergic encephalomyelitis (eae). guinea pigs challenged with virus-myelin emulsions without cfa developed clinical and histological signs o ... | 1979 | 93731 |
| therapeutic control of viral infections: chemotherapy, interferon and gamma globulin. | 1979 | 94280 | |
| comparative studies on ls antigens induced by vaccinia and cowpox viruses. | the constituents of ls antigen from cells infected with vaccinia virus and with cowpox virus were compared by immunoprecipitation and sds-polyacrylamide gel electrophoresis (sds-page). antiserum to the ls antigen from cells infected with vaccinia virus reacted with at least five polypeptides in cells infected with either virus. four of these polypeptides were similar sizes in cells infected with the two viruses. however, one major polypeptide with a mol. wt. of about 100 000 (100 k) detected in ... | 1979 | 94351 |
| cell-mediated cytotoxicity during vaccinia virus revaccination in man: influence of antibodies and interferon. | seven healthy human adults were revaccinated with vaccinia virus. cell-mediated cytotoxicity to vaccinia virus-infected fibroblasts was investigated on the day of vaccination and on days with peak activity. three donors were studied until day 30 after vaccination. the addition of interferon to cytotoxicity reactions resulted in an increase in killing. this increase was not seen when antibodies were added. when a mixture of lymphocytes from a revaccinated and a non-revaccinated donor was used as ... | 1979 | 94462 |
| studies on the mode of inhibition of vaccinial dermal lesions in rabbit. | 1979 | 94591 | |
| fungal nucleic acids as interferon inducers. | nucleic acids isolated from the fungi aspergillus niger x11, piptoporus betulinus and ganoderma applanatum reduced the number of vaccinia virus plaques in chick embryo fibroblast (cef) tissue culture and when administered intravenously to white mice protected them against lethal infection with tick borne encephalitis virus strain k5 (tbe). in cef tissue culture the nucleic acids of the studied fungi were found to induce small but detectable amounts of a substance with the character of interferon ... | 1979 | 94746 |
| [a contribution to the stabilisation of the mva-vaccine (author's transl)]. | the titre of the mva-vaccine remains constant at -20 degrees c with human serum albumin, sorbitol, haemaccel, rheomacrodex and cysteine within 8 month. at -20 degrees c the stability of the mva-virus is equivalent to the vaccinia-virus, stabilized by peptone and sorbitol. storing the mva-virus at +4 degrees c, +20 degrees c for 8 month or at 27 degrees c for 4 weeks, 1% human serum albumin and 5% sorbitol preserves the virustitre best, but the stability of vaccinia-virus with 5% peptone and 5% s ... | 1979 | 94967 |
| cytotoxic macrophages induced in hamsters by vaccinia virus: selective cytotoxicity for virus-infected targets by macrophages collected late after immunization. | 1979 | 87476 | |
| t cell-mediated immune responses of lupus-prone bxsb mice and other murine strains. | cellular-mediated immunity in the newly described bxsb strain of mice, which is prone to autoimmune disease, has been compared with that of two other strains, c57bl/6 and 129/j. quantificaiton of cytotoxic t cell responses to alloantigens and viruses (lymphocytic choriomeningitis and vaccinia virus) showed no difference in the kinetics of appearance and relative activity of cytotoxic t cells per spleen between the young and old bxsb and the control mice. the t cell-dependent primary footpad swel ... | 1979 | 159146 |
| antiviral action of benzo[de]isoquinoline-1,3-diones: 5-nitro-2-(2-dimethylaminoethyl) and 5-nitro-2-[2-(1-pyrrolidine)-ethyl] derivatives. | two benzo[de]isoquinoline-diones, namely 5-nitro-2-(2-dimethylaminoethyl)-benzo[de]isoquinoline-1,3-dione and 5-nitro-2-[2-(1-pyrrolidine)-ethyl]-benzo[de]isoquinoline-1,3-dione, caused inhibition of the viral replication, when assayed against herpes simplex and vaccinia viruses in chick embryo cell cultures. influenza and sindbis virus replication were unaffected by these chemicals. virucidal effects were unobserved. the inhibitory activity is time-related. ocular and dermal infections with vac ... | 1979 | 221156 |
| activation of reticuloendothelial cells following infection with murine cytomegalovirus. | macrophage and reticuloendothelial system function was investigated in adult, female c3h mice during the course of a nonlethal infection with murine cytomegalovirus (mcmv). peritoneal macrophages harvested from mice infected three to 21 days previously were tumoricidal in vitro for a syngeneic mammary tumor cell line and showed antiviral activity by suppressing replication of vaccinia virus in a fibroblast monolayer. in addition, macrophages harvested from animals on days 7 and 14 after mcmv inf ... | 1979 | 221597 |
| vaccinia virus replication requires active participation of the host cell transcriptional apparatus. | the ability of vaccinia virus to replicate in bsc-40 monkey cells whose nuclei have been functionally inactivated was examined. exposure of cell monolayers to ultraviolet radiation at doses that did not alter the cells' capacity to support a subsequent infection by a cytoplasmic virus (vesicular stomatitis virus) caused a reduction to less than 10% in the observed yield of infectious progeny from vaccinia virus and herpes simplex virus (type 1) infections. similarly, replication of vaccinia vir ... | 1979 | 221909 |
| identification of orthopoxviruses by polyacrylamide gel electrophoresis of intracellular polypeptides. i. four major groupings. | 1979 | 222049 | |
| assay of human interferon in vero cells by several methods. | four methods for the assay of human interferon in vero cells were compared based on the inhibition of viral cytopathic effect (cpe) in tubes, the inhibition of cpe in microplates, the reduction of plaques, and the inhibition of quantitative hemadsorption. for inhibition of cpe, sindbis virus, vesicular stomatitis virus, poliovirus type 2, and vaccinia virus were used for challenge. in the plaque reduction method, sindbis virus, vesicular stomatitis virus, and poliovirus were employed, and newcas ... | 1979 | 222803 |
| antiviral activity of aliphatic nucleoside analogues: structure-function relationship. | of a series of 58 aliphatic nucleoside analogues, (s)-9-(2,3-dihydroxypropyl)adenine [(s)-dhpa] proved to be the most active congener, when assayed for antiviral activity in primary rabbit kidney cell cultures challenged with either vaccinia or vesicular stomatitis virus. whereas most analogues derived from substituted purine and pyrimidine bases and bearing various hydroxy- or amino-substituted alkyl chains did not show evidence of antiviral activity at a concentration of 2 mm, (s)-dhpa inhibit ... | 1979 | 222906 |
| synthesis and antiviral activities of arabinofuranosyl-5-ethylpyrimidine nucleosides. selective antiherpes activity of 1-(beta-d-arabinofuranosyl)-5-ethyluracil. | 1979 | 222908 | |
| pantomycin: a new antimicrobial antibiotic. | 1979 | 223049 | |
| [the treatment of advanced malignant melanoma with vaccina oncolysates (author's transl)]. | ten patients with recurrent stage ii or iii malignant melanoma received a live vaccinia virus augmented malignant melanoma cell vaccine. no patient had adverse reactions. the 3 patients surviving (10, 18 and 19 months) had: positive skin reactions to the vaccine (as they had previous positive skin reactions to common recall antigens), elevated vaccinia antibody titers, and they showed lack of progression of the disease with immunotherapy. | 1979 | 223123 |
| transfer of 5'-terminal cap of globin mrna to influenza viral complementary rna during transcription in vitro. | we have recently demonstrated that globin mrnas are effective primers for influenza viral rna transcription in vitro catalyzed by the virion transcriptase [bouloy, m., plotch, s. j. & krug, r. m. (1978) proc. natl. acad. sci. usa 75, 4886-4890]. here, we present direct evidence that the 5'-terminal methylated cap of the globin mrnas is transferred to viral complementary rna (crna) during transcription. chemical (beta-elimination) or enzymatic removal of the cap of globin mrnas eliminated essenti ... | 1979 | 287003 |
| cellular immune response to measles, mumps, and vaccinia viruses in multiple sclerosis. | the cell-mediated immune response to measles, mumps, and vaccinia viruses was studied in ms patients, normal controls, and neurological disease controls using a lymphocyte proliferation assay. a small but significant difference was found in the response to measles between the ms and normal control groups but not between the ms and neurological disease control groups. in each of the groups, the response of both peripheral blood leukocytes and purified t-cells to measles was significantly less tha ... | 1979 | 315204 |
| effect of viruses on luminol-dependent chemiluminescence of human neutrophils. | the effects of newcastle disease, herpes simplex, vaccinia, encephalomyocarditis, vesicular stomatitis and reoviruses on in vitro function of neutrophils were studied in ficoll-hypaque-separated polymorphonuclear leukocytes (pmn) employing the technique of luminol-dependent chemiluminescence. newcastle disease, herpes simplex vaccinia, and reoviruses depressed chemiluminescence by 98, 65, 46, and 29%, respectively, while encephalomyocarditis and vesicular stomatitis viruses had no inhibitory eff ... | 1979 | 223982 |
| effect of aphidicolin on viral and human dna polymerases. | 1979 | 224865 | |
| interferon production by mammalian cells grown in a serum-free medium. | interferon, produced by rabbit heart cells grown in a serum-free medium, failed to protect rabbit heart serum-free cells, but protected rabbit heart serum-containing-medium cells against vaccinia and vesicular stomatitis virus. interferon produced in serum-free cells had a greater species specificity than that produced in serum-containing media. the difference in activity was shown to be due to lack of adsorption by serum-free-medium cells. | 1979 | 225261 |
| plaque formation by a host range mutant of vaccinia virus in non-permissive cells co-infected with yaba virus. | a host-dependent conditional lethal mutant of vaccinia virus strain dis was rescued to form plaques in a non-permissive cell line jinet co-infected with yaba virus, a poxvirus serologically distant from the rescued virus. the efficiency of plaque formation under optimal conditions in this system was comparable to that in vero cells which were permissive for the mutant. the plaque number of the mutant in jinet cells was influenced greatly by the multiplicity of yaba virus, the interval between in ... | 1979 | 225417 |
| replication of vaccinia dna and studies on the structure of the viral chromosome. | 1979 | 226322 | |
| biogenesis of poxviruses: role for the dna-dependent rna polymerase ii of the host during expression of late functions. | the participation of host rna polymerase ii in the vaccinia life cycle was examined by comparing efficiency of multiplication after treating the ama+ sensitive and ama 102 drug resistant lines with alpha-amanitin. in the latter, resistance is due to a mutation in rna polymerase ii. the toxin profoundly reduces synthesis of virus-specified polypeptides and morphopoeisis in ama+ but not in ama 102 rat myoblasts without appreciably altering vaccinia dna replication in either cell type. this implica ... | 1979 | 226996 |
| orthopoxvirus strains defective in surface antigen induction. | various strains of vaccinia, variola, whitepox, monkeypox and cowpox viruses were examined for their capacity to induce a specific early antigen detectable on the surface of infected cells. the elstree strain of vaccinia, two strains of variola minor and white variants of cowpox and monkeypox viruses lacked the capacity to induce the antigen. variation of the parent cowpox and monkeypox viruses to white variants was always accompanied by the loss of the antigen-inducing capacity. | 1979 | 227999 |
| n-alpha-cocoyl-l-arginine ethyl ester, dl-pyroglutamic acid salt, as an inactivator of hepatitis b surface antigen. | n-alpha-cocoyl-l-arginine ethyl ester, dl-pyroglutamic acid salt (cae), exhibited a strong inactivating effect on hepatitis b surface antigen. concentrations of cae required for 50 and 100% inactivation of the antigen were 0.01 to 0.025% and 0.025 to 0.05% respectively. cae completely inactivated hepatitis b surface antigen at the lowest concentration compared with various compounds including about 500 amino acid derivatives, sodium hypochlorite, 2,4,4'-trichloro-2'-hydroxydiphenyl ether, and so ... | 1979 | 228595 |
| comparison of cowpox-like viruses isolated from european zoos. brief report. | poxviruses isolated from captive carnivores in russia (moscow virus) and elephants in germany (elephant virus) were very closely-related to cowpox virus. immunological analysis with absorbed sera separated elephant virus but not cowpox and moscow virus, whereas polypeptide analysis separated cowpox but not elephant and moscow virus. a combination of biological tests separated all three. the epidemiological implications are briefly reviewed. | 1979 | 229799 |
| serologically cross-reactive polypeptides in vaccinia, cowpox and shope fibroma viruses. | an immunoprecipitation method coupled with sds-polyacrylamide gel electrophoresis (sds-page) was used to identify the serologically cross-reactive polypeptides in orthopoxvirus (vaccinia and cowpox viruses) and leporipoxvirus (shope fibroma virus). two early and four late polypeptides in cells infected with vaccinia or cowpox virus were specifically immunoprecipitated with antiserum against shope fibroma virus. two early and two late polypeptides in cells infected with shope fibroma virus cross- ... | 1979 | 230296 |
| 5-substituted 2'-deoxyuridines which selectively inhibit herpes simplex virus replication. | 1979 | 230717 | |
| [inhibition of vaccinia, sindbis and para-influenza virus replication by isatin beta-thiosemicarbazone derivative and methisazone in vitro and in vivo]. | 1979 | 232050 | |
| conservation and variation in orthopoxvirus genome structure. | orthopoxvirus dna from representative strains of rabbitpox, vaccinia, monkeypox, variola, cowpox and ectromelia viruses was analysed by cleavage with restriction endonucleases hindiii, xhoi or smai. genome mol. wt. vary from about 120 x 10(6) for rabbitpox to about 145 x 10(6) for cowpox. physical maps of cleavage sites are similar and characteristic for strains of the same orthopoxvirus type. the distribution of hindiii sites suggests that an internal region of mol. wt. about 30 x 10(6) is high ... | 1979 | 232137 |
| [the resistance of a maternally-derived immunity of baby mice against mousepox--experiments regarding a special possibility of immunization with orthopox viruses (author's transl)]. | in the scope of research concerning new proplylactic possibilities against mousepox we tested the resistance of a passive immunity of baby mice whose mothers had been immunized before or during pregnancy several times with either the vaccinia virus strains mva, cvb or the so-called attenuated ectromelia virus strain "hampstead" respectively. the vaccines cvb and "hampstead" were found to cause the production of haemagglutination-inhibiting antibodies only. a passively-transmitted immunity of bab ... | 1979 | 232602 |
| [analysis of vaccinia virus genome with restriction endonucleases ecori, bamhi, kpni and hindiii]. | restriction endonucleases ecori, bamhi, hind iii and kpni were used for analysis of acccinia virus dna. the number and size of restriction endonuclease fragments were determined by gel electrophoresis and the analysis of 3h-labeled vaccinia virus dna. it was shown that many ecori and bamhi fragments had the same and similar sizes. the exact number of ecori and bamhi fragments were estimated only after analysis of [3h]-labeled vaccinia dna. the vaccinia genome sizes calculated from hindiii, kpni ... | 1979 | 232745 |
| glycyrrhizic acid inhibits virus growth and inactivates virus particles. | screening investigations in antiviral action of plant extracts have revealed that a component of glycyrrhiza glabra roots, found to be glycyrrhizie acid, is active against viruses. we report here that this drug inhibits growth and cytopathology of several unrelated dna and rna viruses, while not affecting cell activity and ability to replicate. in addition, glycyrrhizic acid inactivates herpes simplex virus particles irreversibly. | 1979 | 233133 |
| inhibition of a complete replication cycle of human cytomegalovirus in actinomycin pre-treated cells. | the study of human cytomegalovirus (hcmv) in cultures of human embryo lung fibroblasts, pre-treated with actinomycin d, has shown that under these conditions the virus infection does not proceed beyond the 'early' events of the virus replication cycle. in the same experimental conditions the growth of poliovirus type i, vaccinia virus and herpes simplex type i virus, was completely unaffected. these results suggest that the complete hcmv replication cycle requires some cellular function(s) betwe ... | 1979 | 217966 |
| 3-substituted adenines. in vitro enzyme inhibition and antiviral activity. | the direct alkylation of adenine at the 3 position has been extended to produce series of 3-alkyl-, 3-allyl-, and 3-(substituted benzyl)adenines. when these compounds were tested for enzyme inhibition and antiviral activity in vitro, 3-n-pentyladenine was found to be the most active compound in inhibiting the enzyme dopamine-beta-hydroxylase, and 3-(2-bromobenzyl)adenine showed the most striking inhibition of multiplication of vaccinia virus and of herpes simplex virus in tissue culture. | 1979 | 218008 |
| 5-substituted uracil arabinonucleosides as potential antiviral agents. | 1979 | 218014 | |
| physical characterization of a stomatitis papulosa virus genome: a cleavage map for the restriction endonucleases hindiii and ecori. | the genome of stomatitis papulosa virus (a parapoxvirus) was cleaved with the restriction endonucleases hindiii and ecori, each giving rise to 6 fragments respectively. double digestion with both enzymes resulted in 8 bands, two of which contained dna fragments in double molar concentrations as revealed by reciprocal digests of isolated dna fragments. the genome size, estimated by summation of the molecular weights of the fragments, is approximately 86 x 10(6) daltons, some 30 x 10(6) daltons sm ... | 1979 | 218532 |
| absence of interferon production in a newly established human cell line. | a cell line established from human embryonic lung, hel-r66, was demonstrated to be highly susceptible to herpes simplex virus types 1 and 2, vaccinia virus, newcastle disease virus (ndv), japanese encephalitis virus (jev), western equine encephalitis (wee) virus, sindbis virus, vesicular stomatitis virus (vsv), and rabies virus. the maximal yields of ndv, jev, wee virus, and rabies virus in this cell line exceeded by 2--4 logs those in control human embryonic lung cells. inability of this cell l ... | 1979 | 218901 |
| de novo synthesis of two classes of dna induced by vaccinia virus infection of hela cells. | equilibrium density gradient centrifugation in cscl confirms that dna synthesized after vaccinia virus infection of hela cells is homogeneous in buoyant density and thus in base composition and is similar in this respect to bulk hela cell dna. in contrast, rate sedimentation in alkaline sucrose gradients distinguishes two main classes of virus-induced dna, neither of which can be equated with cell dna synthesized in the same cultures prior to infection. the slower sedimenting class of virus-indu ... | 1979 | 759558 |
| methylation of vaccinia virus-specific mrna in the interferon-treated chick cell. | 1979 | 425328 | |
| effect of capping upon the mrna properties of satellite tobacco necrosis virus ribonucleic acid. | the mrna guanyltransferase-mrna methyltransferases of vaccinia virions can be used to introduce a 5'-terminal m7g(5')pp(5')apm... capping group onto the rna of satellite tobacco necrosis virus (stnv rna) to yield intact capped stnv rna. studies with an in vitro system from wheat germ and limiting quantities of capped and uncapped stnv rna show that the rates and extents of formation of initiation complexes of protein synthesis by intact capped and uncapped stnv rna are identical, suggesting that ... | 1979 | 427119 |
| formation of cross-linked molecules during vaccinia dna replication. | the appearance of cross-linked dna molecules during the replication of vaccinia virus dna was investigated. conversion of full size dna molecules to cross-linked molecules occurs several hours after completion of dna synthesis. inhibition of rna synthesis by actinomycin d did not prevent this transition; however, interruption of protein synthesis by streptovitacin a resulted in partial inhibition of the process. | 1979 | 429148 |
| inhibition of vaccinia virus growth by zinc ions: effects on early rna and thymidine kinase synthesis. | accumulation of thymidine kinase activity in vaccinia virus-infected cells was severely inhibited by zinc ions if the drug was added within 1 h postinfection. if added later, zinc ions had no effect on the enzyme synthesis. a fraction of rna which is normally synthesized in infected cells, was missing from a proper part of the gradient if the cells were treated with zinc ions within 1 h postinfection (as has been shown by cosedimentation of pulse-labeled rnas in isokinetic gradients). it is sugg ... | 1979 | 430599 |
| vaccinia virus infection of hela cells. ii. disparity between cytoplasmic and nuclear viral-specific rna. | 1979 | 442529 | |
| on the inhibitory effect of 2-amino-4,6-dichloropyrimidine on growth of vaccinia virus. | 2-amino-4,6-dichloropyrimidine prevents maturation of vaccinia virus. proteins synthesized in the presence of the drug are not assembled into virions. | 1979 | 446602 |