Publications

schistosoma japonicum showing flat colon polyp appearance.		2010	21195405
rapid identification and differentiation of fasciola hepatica and fasciola gigantica by a loop-mediated isothermal amplification (lamp) assay.	the present study developed and validated a species-specific loop-mediated isothermal amplification (lamp) assay for the rapid detection and discrimination of fasciola hepatica and fasciola gigantica. the lamp assay is inexpensive, easy to perform and shows rapid reaction, wherein the amplification can be obtained in 45 min under isothermal conditions of 61 °c or 62 °c by employing a set of four species-specific primer mixtures and results can be checked through naked-eye visualization. the opti ...	2010	20933335
kinetics of circulating antigen 14-3-3 in sera of rabbits firstly infected with schistosoma japonicum and treated with/without praziquantel.	a sandwich elisa was developed for the detection of circulating antigen 14-3-3 in the sera of rabbits. rabbits that were infected with 500 cercariae of schistosoma japonicum were grouped and the kinetics of 14-3-3 was observed. for the treated group, the 14-3-3 protein could be detected as early as 2-4 weeks postinfection and then its levels rose rapidly and reached a peak at around 6 weeks. the 14-3-3 levels in the sera significantly decreased after the infected rabbits were treated with praziq ...	2010	20953628
genome-wide analyses reveal a role for peptide hormones in planarian germline development.	bioactive peptides (i.e., neuropeptides or peptide hormones) represent the largest class of cell-cell signaling molecules in metazoans and are potent regulators of neural and physiological function. in vertebrates, peptide hormones play an integral role in endocrine signaling between the brain and the gonads that controls reproductive development, yet few of these molecules have been shown to influence reproductive development in invertebrates. here, we define a role for peptide hormones in cont ...	2010	20967238
study on differences in the pathology, t cell subsets and gene expression in susceptible and non-susceptible hosts infected with schistosoma japonicum.	more than 40 kinds of mammals in china are known to be naturally infected with schistosoma japonicum (s. japonicum); microtus fortis (m. fortis), a species of vole, is the only mammal in which the schistosomes cannot mature or cause significant pathogenic changes. in the current study, we compared the differences in pathology by hematoxylin-eosin staining and in changes in the t cell subsets with flow cytometry as well as gene expression using genome oligonucleotide microarrays in the lung and l ...	2010	20976156
immune events associated with high level protection against schistosoma japonicum infection in pigs immunized with uv-attenuated cercariae.	the vaccination of radiation-attenuated schistosoma japonicum cercariae can induce effective protection in artiodactyl, but the immune events related to protective immunity are not fully understood. to provide a paradigm for a human recombinant antigen vaccine, we have undertaken a vaccination and challenge experiment in pigs, which was recognized as an appropriate animal model in this type of study because of their similarity to human in immunology, and investigated the relative immune events i ...	2010	20976218
no evidence for lateral gene transfer between salmonids and schistosomes.		2010	20980981
[construction and expression of recombinant plasmid pet28alpha-sj26gst of schistosoma japonicum in escherichia coli bl21].	to construct and express recombinant plasmid pet28a-sj26gst of schistosoma japonicum (sj) in escherichia coli bl21 (de3).	2010	21302435
spatially explicit agent-based modelling for schistosomiasis transmission: human-environment interaction simulation and control strategy assessment.	as the transmission of many other parasitic diseases, the transmission of schistosomiasis is a complex process governed by natural, socio-economic factors and human life style. based on the life cycle of schistosoma japonicum, some models have been developed. however, the human-environment interaction, especially through agricultural activities, has not been explicitly modeled in previous efforts.	2010	21352776
[comparative evaluation of five test kits for antibody detection in schistosoma japonicum endemic areas of poyang lake region].	to evaluate the efficacy of five diagnostic test kits for the detection of antibodies to schistosoma japonicum in endemic areas of poyang lake.	2010	21500532
antigen presenting cells may be able to distinguish between normal and radiated schistosoma japonicum cercaria: an in vitro observation.	to observe the discrepancies of responses induced by schistosoma japonicum (s. japonicum) normal cercaria antigen (nca) and ultraviolet (uv) -radiation-attenuated cercaria antigen (uvaca) in an in vitro system.	2010	23554642
ultraviolet- attenuated cercariae of schistosoma japonicum fail to effectively induce a th1 response in spite of up-regulating expression of cytotoxicity-related genes in c57bl/6 mice.	to better understand the reason that schistosoma japonicum (s. japonicum) ultraviolet (uv)-radiated cercariae could not induce high level of protection in c57bl/6 mice.	2010	23554641
[effect of n-acetylcysteine on malondialdehyde and superoxide dismutase in hepatic tissue of mice with schistosomiasis japonica].	90 mice were randomly divided into six groups: normal control, infected control, long-term drug use group 1 (l1), long-term drug use group 2 (l2), short-term drug use group 1 (s1) and short-term drug use group 2 (s2). mice in all groups except those in the normal control group were infected with 30 cercariae of schistosoma japonicum through abdominal skin. n-acetylcysteine (nac) solution was orally given to mice in l1 and l2 groups, 200 mg/kg and 400 mg/kg, respectively, 2 times/d from the day o ...	2011	24822348
[inhibition of culture supernatant of mesenchymal stem cells on macrophages raw264.7 activated by soluble egg antigen of schistosoma japonicum].	to observe the inhibitive effect of rat mesenchymal stem cells (msc) culture supernatant on macrophages activated by soluble egg antigen (sea) of schistosoma japonicum.	2011	24822341
development of a double antibody sandwich elisa assay for the diagnosis of angiostrongyliasis.	abstract abstractusing two monoclonal antibodies (mabs) against excretory/secretory (es) antigens of angiostrongylus cantonensis adult worms, a method of double antibody sandwich elisa for the detection of circulating antigens (cag) was established with the best experimental conditions regulated for performance. to evaluate the sensitivity, the cag in sera of rats (80) and mice (15) infected with a. cantonensis as well as cag in sera of clinical confirmed angiostrongyliasis patients (70) were de ...	2011	21506824
mapping the binding between the tetraspanin molecule (sjc23) of schistosoma japonicum and human non-immune igg.	schistosomal parasites can establish parasitization in a human host for decades; evasion of host immunorecognition including surface masking by acquisition of host serum components is one of the strategies explored by the parasites. parasite molecules anchored on the membrane are the main elements in the interaction. sjc23, a member of the tetraspanin (tsp) family of schistosoma japonicum, was previously found to be highly immunogenic and regarded as a vaccine candidate against schistosomiasis. ...	2011	21533061
electrophoretic detection of genetic variability among schistosoma japonicum isolates by sequence-related amplified polymorphism.	in the present study, sequence-related amplification polymorphism (srap) was utilized to study the genetic variability among schistosoma japonicum isolates from different provinces in china, using schistosoma mansoni from puerto rico for comparison. five out of ten tested srap primer combinations displayed significant polymorphisms among s. japonicum isolates from china, namely me2/em1, me4/em1, me4/em6, me5/em4 and me5/em5. analysis of the 61 s. japonicum samples from china with five srap prime ...	2011	21538983
[the protective effect of chronic schistosoma japonica infestation against sepsis in mice.]	objective:to preliminarily study the protective effect of chronic schistosoma japonica (sj) infestation against sepsis in mice and its mechanism. methods:balb/c male mice were used, and the experiment was divided into three parts. experiment 1: chronic sj infestation model was reproduced by sj cercaria inoculation through abdominal skin for 8 weeks. twenty mice were randomly grouped into normal group (n=10) and sj group (n=10). the levels of interleukins (il-4, il-10),tumor necrosis factor-α (tn ...	2011	21549067
cloning and characterization of a novel enzyme: tyrosine hydroxylase from schistosoma japonicum.	catecholamines, such as dopamine and noradrenaline, play important roles as neuromuscular transmitters and modulators in all parasitic helminthes, including schistosoma japonicum. s. japonicum tyrosine hydroxylase (sjth) was amplified by rapid amplification of cdna ends polymerase chain reaction that shows strong homology to schistosoma mansoni tyrosine hydroxylase, the enzyme that catalyzes the first and rate-limiting step in the biosynthesis of catecholamines. the sjth transcripts encoded the ...	2011	21556690
proteomic analysis of schistosoma japonicum schistosomulum proteins that are differentially expressed among hosts differing in their susceptibility to the infection.	schistosomiasis is a tropical, parasitic disease affecting humans and several animal species. the aim of this study was to identify proteins involved in the growth and survival of the parasitic forms inside a host. schistosomula of schistosoma japonicum were isolated from three different hosts: the susceptible balb/c mice; the wistar rats, which have a considerably lower susceptibility; and the resistant reed vole, microtus fortis. soluble proteins of the schistosomula collected from the above t ...	2011	21593212
performance of a dipstick dye immunoassay for rapid screening of schistosoma japonicum infection in areas of low endemicity.	abstract: background: the dipstick dye immunoassay (ddia), recently commercially available in the people's republic of china (p.r. china), is a rapid and simple test to detect human antibodies against schistosoma japonicum. its performance and utility for screening schistosome infection in low endemic areas is little known. we therefore carried out a cross-sectional survey in seven villages with low endemicity of schistosomiasis in p.r. china and assessed the performance and utility of ddia for ...	2011	21599944
characterization and expression of a novel cystatin gene from schistosoma japonicum.	cystatins are a family of cysteine protease inhibitors that play a crucial role in the immune evasion from their host and in the adaptation to host defence. here, we isolated a full-length cdna sequence inferred to encode a novel cystatin gene from a blood fluke, schistosoma japonicum. the cdna, designated sjcystatin, comprised an open reading frame (orf) of 306 bp, and encoded 101 amino acids with a predicted molecular weight of 11.3 kda. this predicted protein shared a significant degree of se ...	2011	21601634
a novel liquid-phase piezoelectric immunosensor for detecting schistosoma japonicum circulating antigen.	aims: a new liquid-phase piezoelectric immunosensor (lp-peis), which can detect schistosoma japonicum (sj) circulating antigens (sjcag) quantificationally, was developed. methods: the igg antibodies were purified from the sera of rabbits which had been infected or immunized by sj and were immobilized on the surface of piezoelectric quartz crystal in lp-peis by staphylococcal protein a (spa). it was used to detect sjcag in sera of rabbits which had been infected by sj in order to acquire some opt ...	2011	21609782
detection of the circulating antigen 14-3-3 protein of schistosoma japonicum by time-resolved fluoroimmunoassay in rabbits.	abstract: background: schistosomiasis remains a major public health concern that afflicts millions of people worldwide. low levels of schistosoma infection require more sensitive diagnostic methods. in this study, a time-resolved fluoroimmunoassay (trfia) was developed for detecting the signal transduction protein 14-3-3, a circulating antigen of schistosoma japonicum. results: the detection limit of 14-3-3-trfia was 0.78 ng/ml, with a linear measurement range from 0.78 to 800 ng/ml. the average ...	2011	21619661
[Dynamic changes of early immune responses to attenuated Schistosoma japonicum cercariae induced in BALB/c mice].	To study the early immune activation and its dynamic changes between the attenuated cercariae immunized mice and the normal infected mice.	2011	22164856
A strategy for emergency treatment of Schistosoma japonicum-infested water.	Schistosomiasis japonica, caused by contact with Schistosoma japonicum cercaria-infested water when washing, bathing or production, remains a major public-health concern in China. The purpose of the present study was to investigate the effect of a suspension concentrate of niclosamide (SCN) on killing cercaria of S. japonicum that float on the water surface, and its toxicity to fish, so as to establish an emergency-treatment intervention for rapidly killing cercaria and eliminating water infecti ...	2011	22047607
dynamics of th17 cells and their role in schistosoma japonicum infection in c57bl/6 mice.	the current knowledge of immunological responses to schistosomiasis, a major tropical helminthic disease, is insufficient, and a better understanding of these responses would support vaccine development or therapies to control granuloma-associated immunopathology. cd4(+) t cells play critical roles in both host immune responses against parasitic infection and immunopathology in schistosomiasis. the induction of t helper (th)1, th2 and t regulatory (treg) cells and their roles in schistosome infe ...	2011	22102924
The cell biology of schistosomes: a window on the evolution of the early metazoa.	This review of schistosome cell biology has a dual purpose; its intent is to alert two separate research communities to the activities of the other. Schistosomes are by far and away the best-characterised platyhelminths, due to their medical and economic importance, but seem to be almost totally ignored by researchers on the free-living lower metazoans. Equally, in their enthusiasm for the parasitic way of life, schistosome researchers seldom pay attention to the work on free-living animals that ...	2011	21976269
[endemic situation of schistosomiasis in guangdong province from 2004 to 2009].	to understand the endemic situation of schistosomiasis in guangdong province.	2011	22164627
characterization of micrornas in taenia saginata of zoonotic significance by solexa deep sequencing and bioinformatics analysis.	the beef tapeworm taenia saginata infects human beings with symptoms ranging from nausea, abdominal discomfort to digestive disturbances and intestinal blockage. in the present study, microrna (mirna) expressing profile in adult t. saginata was analyzed using solexa deep sequencing and bioinformatics analysis. a total of 15.8 million reads was obtained by solexa sequencing, and 13.3 million clean reads (1.73 million unique sequences) was obtained after removing reads smaller than 18 nt. ten cons ...	2011	22203522
identification, structure and function of a novel tetraspaninhomologue from spirometra erinaceieuropaei.	to identify a full length c dna sequence of a novel tetraspanin (tsp) homologue from spirometra erinaceieuropaei and to predict the structure and function of its encoding protein using bioinformatics methods.	2011	21967699
Schistosoma japonicum: treatment of different developmental stages in mice with long-acting praziquantel implants.	This paper reports the effective treatment of Schistosoma japonicum in a mouse model with long-acting praziquantel (PZQ)-loaded poly(e-caprolactone) implants. The implants yielded stable, high plasma PZQ concentrations ranging 100-1600 ng/mL during the 40-day investigation period. For assessment of efficacy, the implants were implanted into mice immediately after infection and at 1, 2, 3 and 4 weeks after infection to treat the schistosomes at different developmental stages. All the mice were sa ...	2011	21856298
[Detection of urine antibodies to Schistosoma japonicum by magnetic particle affinity immunoassay].	To explore a non-invasive method for detection of urine antibodies to Schistosoma japonicum.	2011	22164507
Gastrointestinal neoplasia associated with bowel parasitosis: real or imaginary?	Several parasitic species are well known to have carcinogenic properties, namely; Schistosoma hematobium (squamous cell carcinoma of the bladder) and the liver flukes Opisthorchis and Chlonorchis (cholangiocarcinoma). A large number of parasites are known to colonize the gastrointestinal tract. We sought to review the evidence that implicates these parasites in gastrointestinal neoplasia. Schistosoma japonicum, which is endemic primarily in east Asia, has been shown in multiple studies to convey ...	2011	22174720
histopathological changes of juvenile schistosoma japonicum harbored in mice treated orally with mefloquine at a smaller single dose.	the histopathological changes of 14-day-old schistosoma japonicum induced by a smaller single dose of mefloquine have been studied. twenty-three mice infected with 60-80 s. japonicum cercariae for 14 days were treated orally with mefloquine at a single dose of 200 mg/kg (free base), and groups of three mice were killed at various intervals posttreatment. the liver of each mouse was removed, fixed and processed routinely, and examined by light microscopy. eight hours posttreatment, 38.2% and 39.8 ...	2011	22190126
[Destructive effect of eggs of Schistosoma japonicum on spleens in C57BL/6 mice].	To observe the destructive effect of eggs of Schistosomajaponicum on the splenic structure in C57BL/6 mice.	2011	22164493
Awareness and knowledge of schistosomiasis infection and prevention in the "Three Gorges Dam" reservoir area: a cross-sectional study on local residents and health personnel.	Schistosomiasis is a severe public health problem in China. It has been predicted that the ecological changes caused by the "Three Gorges Dam", the world's largest hydropower project, could potentially aggravate the spread of schistosomiasis in the area. This study focused on investigating (a) local residents' knowledge on the potential risks of schistosomiasis and (b) the capability of local health personnel in preventing schistosomiasis.	2011	21945272
the complete mitochondrial genome of orientobilharzia turkestanicum supports its affinity with african schistosoma spp.	orientobilharzia turkestanicum is a blood fluke of many mammals and causes orientobilharziasis that is also a neglected parasitic zoonosis because the cercaria of o. turkestanicum can infect humans and cause cercarial dermatitis. the present study determined the complete sequence of mt genome of o. turkestanicum and revised its phylogenetic position based on mt gene content and arrangement. the complete mtdna sequence of o. turkestanicum was 14,755 bp in length, which is slightly larger than the ...	2011	21930247
schistosomiasis research in the dongting lake region and its impact on local and national treatment and control in china.	schistosomiasis is a chronic and debilitating parasitic disease that has often been neglected because it is a disease of poverty, affecting poor rural communities in the developing world. this is not the case in the people's republic of china (prc), where the disease, caused by schistosoma japonicum, has long captured the attention of the chinese authorities who have, over the past 50-60 years, undertaken remarkably successful control programs that have substantially reduced the schistosomiasis ...	2011	21912706
[application of f-elisa for immunodiagnosis of schistosomiasis japonica].	to optimize the experimental conditions of fast enzyme-linked immunosorbent assay (f-elisa) and evaluate its performance for immunodiagnosis of schistosomiasis japonica.	2011	22164616
the challenge of effective surveillance in moving from low transmission to elimination of schistosomiasis in china.	until recently, intensified efforts in china to suppress the transmission of schistosoma japonicum relied principally on routine praziquantel treatment, extensive use of molluscicides and health education programs. these efforts, now supplemented by a broader range of control measures, have been quite successful in reducing the prevalence and intensity of human infection to very low levels. however, re-emergent transmission has occurred in formerly endemic areas of several provinces, signalling ...	2011	21920366
[surveillance and forecast system of schistosomiasis in jiangsu province. iv. establishment of schistosoma japonicum cercaria-killing method by spraying niclosamide suspension on water surface].	to investigate the effect of suspension concentrate of niclosamide (scn) on killing cercariae of schistosoma japonicum on water surface, optimization and impact on fish, so as to establish an emergency-treatment intervention for rapidly killing cercariae and eliminating water infectivity.	2011	22164370
[epidemiological survey of suspected natural foci of schistosomiasis in luxi city].	three schistosomiasis patients pathologically diagnosed were detected in luxi city, yunnan province, a non-endemic area of schistosomiasis. all the cases were hospitalized for serious intestinal diseases, and schistosoma japonicum eggs were detected under their colonic mucosa. the 3 patients all denied they had contacted with infested water outside their resident place, and it was suggested that they might be infected in the local area.	2011	22164393
[diagnostic efficiency of indirect hemagglutination assay kit for antibody detection of schistosomiasis japonica].	to evaluate the clinical diagnostic efficiency of indirect hemagglutination assay kit (iha kit) for antibody detection of schistosomiasis japonica.	2011	22164845
[preliminary study on the immunological characteristics of permissive and non-permissive hosts infected with schistosoma japonicum].	to study the difference among immune responses of three kinds of experimental animals with different susceptibility to the infection of schistosoma japonicum, and preliminarily explore the mechanism of the immune response in permissive and non-permissive hosts.	2011	21972600
clinics in diagnostic imaging (136). chronic schistosoma japonica infection of the liver.	a 37-year-old man from china was initially seen at the urology clinic for symptoms of urinary colic. plain computed tomography (ct) imaging of the kidneys, ureters and bladder was performed, which (in addition to demonstrating renal calculi) revealed incidental findings of 'turtleback' septal and capsular calcifications, features pathognomonic for schistosomiasis japonica. other classical features were demonstrated on the triphasic hepatic ct imaging that was subsequently performed. the clinical ...	2011	21947150
anthelmintic effect of artesunate in experimental heterophyid infection.	artesunate is a water-soluble semi-synthetic drug derived from artemisinin which is extracted from leaves of the chinese plant artemisia annua. this successful basically-antimalarial drug has been proved to be also effective against trematodes like schistosoma japonicum, s. mansoni, fasciola hepatica and clonorchis sinensis. the present work aimed to investigate the in-vivo efficacy of artesunate against heterophyids in mice, using praziquantel as a therapeutic control. results showed that artes ...	2011	21980784
confocal laser scanning microscopic observation on adult schistosoma japonicum harbored in mice following treatment with single-dose mefloquine.	the aim of the present study is to assess the mefloquine-induced alteration of adult schistosoma japonicum using confocal laser scanning microscopy (clsm). eight out of ten mice infected with 60-80 s. japonicum cercariae for 35 days were treated orally with mefloquine at a single dose of 400 mg/kg. four groups of two mice were killed at 24 h and 3, 7, and 14 days post-treatment, and schistosomes were collected by perfusion from the liver and mesenteric veins, fixed in 70% alcohol, stained with a ...	2011	22200961
[evaluation of kato-katz technique combined with stool hatching test in diagnosis of schistosomiasis japonica].	to develop an aetiology detection method with higher sensitivity and accuracy for schistosomiasis japonica.	2011	22164505
recent advances in schistosoma genomics.	schistosome research has entered the genomic era with the publications reporting the s. mansoni and s. japonicum genomes. schistosome genomics is motivated by the need for new control tools. however, much can also be learned about the biology of schistosoma, which is a tractable experimental model. in this paper, we review the recent achievements in the field of schistosome research and discuss future perspectives on genomics and how it can be integrated in a usable format, on the genetic mappin ...	2011	22145587
establishing transgenic schistosomes.		2011	21912709
identifying schistosoma japonicum excretory/secretory proteins and their interactions with host immune system.	schistosoma japonicum is a major infectious agent of schistosomiasis. it has been reported that large number of proteins excreted and secreted by s. japonicum during its life cycle are important for its infection and survival in definitive hosts. these proteins can be used as ideal candidates for vaccines or drug targets. in this work, we analyzed the protein sequences of s. japonicum and found that compared with other proteins in s. japonicum, excretory/secretory (es) proteins are generally lon ...	2011	21887319
[effects of male worm extraction on ultrastructure of cultured vitelline cells from schistosoma japonicum].	to study the effects of male worm extraction on the proliferation and metabolic activity of cultured vitelline cells from schistosoma japonicum.	2011	22164857
quantifying quality of life and disability of patients with advanced schistosomiasis japonica.	the chinese government lists advanced schistosomiasis as a leading healthcare priority due to its serious health and economic impacts, yet it has not been included in the estimates of schistosomiasis burden in the global burden of disease (gbd) study. therefore, the quality of life and disability weight (dw) for the advanced cases of schistosomiasis japonica have to be taken into account in the re-estimation of burden of disease due to schistosomiasis.	2011	21358814
expression, characterization and therapeutic efficacy of chimeric fab of anti-idiotypic antibody np30 against schistosoma japonicum.	the murine monoclonal anti-idiotypic antibody np30 is a promising therapeutic antibody against schistosoma japonicum. however, the immunogenicity of murine np30 limits its further study and application in humans. here the chimeric fab of np30 (chfab-np30) comprising the variable regions of murine np30 and constant regions of human antibody was assembled. chfab-np30 was expressed and purified as a soluble and functional protein. administration of chfab-np30 in vivo increased the survival rate, re ...	2011	21376700
toll-like receptor (tlr) 2 and tlr4 deficiencies exert differential in vivo effects against schistosoma japonicum.	little is known about the functions of toll-like receptor 2 (tlr2) and tlr4 in innate/acquired responses to schistosoma japonicum. through in vivo study, the contributions of tlr2 and tlr4 to host immune responses during s. japonicum infection were investigated. early infection experiments showed higher protein and mrna levels of il-12, ifn-+¦ and il-4 in dermal tissues and retroauricular draining lymph nodes respectively in tlr2(-/-) mice on day four post-infection and opposite changes in tlr4( ...	2011	21392041
in-vivo activity of dihydroartemisinin against schistosoma japonicum.		2011	21396254
evaluation of mammalian and intermediate host surveillance methods for detecting schistosomiasis reemergence in southwest china.	schistosomiasis has reemerged in china, threatening schistosomiasis elimination efforts. surveillance methods that can identify locations where schistosomiasis has reemerged are needed to prevent the further spread of infections.	2011	21408127
a 5-year longitudinal study of schistosomiasis transmission in shian village, the anning river valley, sichuan province, the peoples' republic of china.	schistosoma japonicum is a major public health concern in the peoples' republic of china (prc), with over one million people infected and another 50 million living in areas at risk of infection. based on ecological, environmental, population genetic and molecular factors, schistosomiasis transmission in prc can be categorised into four discrete ecosystems or transmission modes. it is predicted that the three gorges dam (tgd) will impact upon the transmission of schistosomiasis in the prc, with v ...	2011	21429229
vaccine potential of hemocyanin from oncomelania hupensis against schistosoma japonicum.	hemocyanin, a giant oxygen transport protein which is usually found in many arthropods and mollusks was isolated and purified from oncomelania hupensis. in this study, we showed that oncomelania hupensis hemocyanin (ohh) shared carbohydrate epitopes with different developmental stages of schistosoma japonicum (cercaria, schistosomulum, adult worm and egg) and exhibited serological cross-reaction with these stages of s. japonicum immune sera, which had a potential for use in diagnostic and therap ...	2011	21440665
establishment of a cerebral schistosomiasis experimental model in rabbits.	the present study aimed to establish a cerebral schistosomiasis model in rabbits, to provide a valuable tool for morphological analysis, clinical manifestation observation, as well as investigations into immunological reactions and pathogenesis of focal inflammatory reaction in neuroschistosomiasis (ns).	2011	21441970
discovery and molecular characterization of a bcl-2-regulated cell death pathway in schistosomes.	schistosomiasis is an infectious disease caused by parasites of the phylum platyhelminthe. here, we describe the identification and characterization of a bcl-2-regulated apoptosis pathway in schistosoma japonicum and s. mansoni. genomic, biochemical, and cell-based mechanistic studies provide evidence for a tripartite pathway, similar to that in humans including bh3-only proteins that are inhibited by prosurvival bcl-2-like molecules, and bax/bak-like proteins that facilitate mitochondrial outer ...	2011	21444803
in silico prediction of binding of promiscuous peptides to multiple mhc class-ii molecules identifies the th1 cell epitopes from secreted and transmembrane proteins of schistosoma japonicum in balb/c mice.	it has not so far been possible to identify rapidly and effectively the anti-schistosomiasis th cell epitopes that are capable of simulating ifn-? (interferon-gamma)-mediated th1-type protective immunity in response to radiation-attenuated schistosome cercaria. with the advance of the omics studies of schistosomes, an approach that used reverse vaccinology probably resolved the above problems. in this "proof-of-principle" study, first, we selected 31 secreted or transmembrane protein sequences s ...	2011	21458582
assessing the impact of misclassification error on an epidemiological association between two helminthic infections.	polyparasitism can lead to severe disability in endemic populations. yet, the association between soil-transmitted helminth (sth) and the cumulative incidence of schistosoma japonicum infection has not been described. the aim of this work was to quantify the effect of misclassification error, which occurs when less than 100% accurate tests are used, in sth and s. japonicum infection status on the estimation of this association.	2011	21468317
mammalian apoptosis in a parasitic worm.		2011	21493866
global expression analysis revealed novel gender-specific gene expression features in the blood fluke parasite schistosoma japonicum.	schistosoma japonicum is one of the remarkable platyhelminths that are endemic in china and southeast asian countries. the parasite is dioecious and can reside inside the host for many years. rapid reproduction by producing large number of eggs and count-react host anti-parasite responses are the strategies that benefit long term survival of the parasite. praziquantel is currently the only drug that is effective against the worms. development of novel antiparasite reagents and immune-prevention ...	2011	21494327
artificial preparation, indoor passage, and nature breed of oncomelania hupensis infected with schistosoma japonicum.	to prepare the infected oncomelania hupensis by artificial method for the research on the activity, vaccine, and genetic variation of schistosoma japonicum (s. japonicum).	2011	21311132
a survey of schistosome protein domain types: insights into unique biological properties.	using the prosite database and search tools, we conducted a comprehensive bioinformatic analysis of the predicted protein sequences of the flatworm parasites schistosoma mansoni and schistosoma japonicum and seven other animal genomes in order to identify novel schistosome-specific features. our analyses revealed a relative paucity of proline-rich domains in schistosomes in comparison with their human host and a corresponding enrichment in schistosomes of asparagine-rich, serine-rich, and threon ...	2011	21315771
transdermal evaporation delivery system of praziquantel for schistosomiasis japonicum chemotherapy.	a transdermal evaporation delivery system (teds) of praziquantel (pzq) was developed by selecting ethylene glycol monophenyl ether as a nonvolatile component solvent and ethanol as a volatile component solvent to control efficiently the transmission and morbidity of the global schistosomiasis, providing a convenient administration system of pzq for both humans and domestic animals. the solubility of pzq in teds was more than 400 mg/ml when the ethanol concentration was 50% (w/w) in the solvent m ...	2011	21319162
molecular cloning, characterization, and immunolocalization of two lactate dehydrogenase homologous genes from taenia solium.	two novel genes encoding lactate dehydrogenase a (ldha) and b (ldhb) homologues, respectively, were identified from the cdna libraries of adult taenia solium (t. solium). the two deduced amino acid sequences both show more than 50% identity to the homologues for danio rerio, xenopus laevis, schistosoma japonicum, sus scrofa, homo sapiens, et al. the identity of the amino acid sequence between tsldha and tsldhb is 57.4%, and that of the nucleotide sequence is 61.5%. recombinant tsldha homologue ( ...	2011	21331787
a coproantigen diagnostic test for strongyloides infection.	accurate diagnosis of infection with the parasite strongyloides stercoralis is hampered by the low concentration of larvae in stool, rendering parasitological diagnosis insensitive. even if the more sensitive agar plate culture method is used repeated stool sampling is necessary to achieve satisfactory sensitivity. in this manuscript we describe the development of a coproantigen elisa for diagnosis of infection. polyclonal rabbit antiserum was raised against strongyloides ratti excretory/secreto ...	2011	21347447
molecular detection of schistosoma japonicum in infected snails and mouse faeces using a real-time pcr assay with fret hybridisation probes.	a real-time polymerase chain reaction (pcr) assay with fluorescence resonance energy transfer (fret) hybridisation probes combined with melting curve analysis was developed to detect schistosoma japonicum in experimentally infected snails and in faecal samples of infected mice. this procedure is based on melting curve analysis of a hybrid between an amplicon from the s. japonicum internal transcribed spacer region 2 sequence, which is a 192-bp s. japonicum-specific sequence, and fluorophore-labe ...	2011	22124555
toward sustainable and comprehensive control of schistosomiasis in china: lessons from sichuan.	triggered by a fascinating publication in the new england journal of medicine detailing china's new multi-pronged strategy to control and eventually interrupt the transmission of schistosoma japonicum, this plos neglected tropical diseases debate critically examines the generalizability and financial costs of the studies presented from the marshlands of the lake region. edmund seto from the university of california and colleagues emphasize that the epidemiology and control of schistosomiasis var ...	2011	22039563
[serological detection of cryptosporidium spp. infection in outpatients in changchun].	cp23 gene of cryptosporidium parvum was expressed in escherichia coli, and the recombinant protein was purified. its immunoreactivity was analyzed by western blotting. serum samples were collected from outpatients of different ages from august to november, 2010 in changchun. indirect elisa was established to detect the anti-cp23 igg in sera. western blotting analysis indicated that the recombinant cp23 protein was recognized by sera from cryptosporidium panum-infected calves and positive human s ...	2011	21970120
insights into the functional biology of schistosomes.	the need to discover new treatments for human schistosomiasis has been an important driver for molecular research on schistosomes, a major breakthrough being the publication of the schistosoma mansoni and schistosoma japonicum genomes in 2009. this 'primer' considers recent advances in the understanding of schistosome biology by providing a snapshot of selected areas of contemporary functional schistosome research, including that on the genome, the tegument, cell signalling and developmental bio ...	2011	22013990
[Susceptibilities of Oncomelania hupensis snails to Schistosoma japonicum miracidia from different hosts].	To understand the susceptibilities of Oncomelania hupensis snails to Schistosoma japonicum miracidia from different hosts.	2011	22164864
[bioinformatics analysis of nicotinamide phosphoribosyl transferase of schistosoma japonicum].	to perform the bioinformatics analysis of nicotinamide phosphoribosyl transferase of schistosoma japonicum so as to provide the evidence for the development and application of it.	2011	22164495
proteomic analysis of human skin treated with larval schistosome peptidases reveals distinct invasion strategies among species of blood flukes.	skin invasion is the initial step in infection of the human host by schistosome blood flukes. schistosome larvae have the remarkable ability to overcome the physical and biochemical barriers present in skin in the absence of any mechanical trauma. while a serine peptidase with activity against insoluble elastin appears to be essential for this process in one species of schistosomes, schistosoma mansoni, it is unknown whether other schistosome species use the same peptidase to facilitate entry in ...	2011	21980548
monitoring specific antibody responses against the hydrophilic domain of the 23 kda membrane protein of schistosoma japonicum for early detection of infection in sentinel mice.	schistosomiasis remains an important public health problem throughout tropical and subtropical countries. humans are infected through contact with water contaminated with schistosome cercariae. therefore, issuing early warnings on the risk of infection is an important preventive measure against schistosomiasis. sentinel mice are used to monitor water body infestations, and identifying appropriate antibody responses to schistosome antigens for early detection of infection would help to improve th ...	2011	21906319
Application of DNA-based diagnostics in detection of schistosomal DNA in early infection and after drug treatment.	Research is now focused on identification of sensitive and specific diagnostic tests for early identification of schistosomal infection and evaluation of chemotherapy in field situations in China.	2011	21864384
stirred, not shaken: genetic structure of the intermediate snail host oncomelania hupensis robertsoni in an historically endemic schistosomiasis area.	oncomelania hupensis robertsoni is the sole intermediate host for schistosoma japonicum in western china. given the close co-evolutionary relationships between snail host and parasite, there is interest in understanding the distribution of distinct snail phylogroups as well as regional population structures. therefore, this study focuses on these aspects in a re-emergent schistosomiasis area known to harbour representatives of two phylogroups - the deyang-mianyang area in sichuan province, china ...	2011	22029536
Effect of the in vivo activity of dihydroartemisinin against Schistosoma mansoni infection in mice.	Dihydroartemisinin, formerly known as an antimalarial drug, is the main metabolite of the mother compound artemisinins, as well as of artemether and artesunate. It has been shown that the drug exhibits antischistosomal efficacy against Schistosoma japonicum. The purpose of the current study was to assess the in vivo effect of dihydroartemisinin against Schistosoma mansoni infection in mice. Drugs at a single oral dose of 300 mg/kg were given to mice to assess the efficacy against different devel ...	2011	22006193
Polymorphism associated with the Schistosoma mansoni tetraspanin-2 gene.	A vaccine against schistosomiasis would contribute significantly to reducing the 3-70 million disability-adjusted life years lost annually to the disease. Towards this end, inoculation with the large extracellular loop (EC-2) of Schistosoma mansoni tetraspanin-2 protein (Sm-TSP-2) has proved effective in reducing worm and egg burdens in S. mansoni-infected mice. The EC-2 loop of Schistosoma japonicum TSP-2, however, has been found to be highly polymorphic, perhaps diminishing the likelihood that ...	2011	21889508
molecular cloning and characterization of a hsp70 gene from schistosoma japonicum.	schistosoma japonicum is the pathogen responsible for schistosomiasis japonica, one of the major infectious diseases targeted for prevention nationally in china. expression of heat shock proteins (hsps) following stress plays a very important biological role in many organisms including s. japonicum. among the hsp family, the 70-kda hsps are most responsible for intracellular chaperone and extracellular immunoregulatory functions. based on the published sequences in genbank/embl (af044412.1), ope ...	2011	22076051
a diagnostic challenge for schistosomiasis japonica in china: consequences on praziquantel-based morbidity control.	worldwide schistosomiasis continues to be a serious public health problem. over the past five decades, china has made remarkable progress in reducing schistosoma japonicum infections in humans to a relatively low level. endemic regions are currently circumscribed in certain core areas where re-infection and repeated chemotherapy are frequent. at present, selective chemotherapy with praziquantel is one of the main strategies in china's national schistosomiasis control program, and thus diagnosis ...	2011	21981948
schistosomal glomerulopathy and changes in the distribution of histological patterns of glomerular diseases in bahia, brazil.	distinct patterns of glomerular lesions, including membranoproliferative glomerulonephritis and focal segmental glomerulosclerosis, are associated with infection by schistosoma mansoni or schistosoma japonicum. evidence suggests that immune complex deposition is the main mechanism underlying the different forms of schistosomal glomerulonephritis and that immune complex deposition may be intensified by portal hypertension. the relationship between focal segmental glomerulosclerosis and schistosom ...	2011	22124564
the sensitivity of artesunate against schistosoma japonicum decreased after 10 years of use in china?	artesuante (as) is a good chemoprophylactic drug for preventing against schistosome infection, but hua et al. recently reported that the sensitivity of as against schistosoma japonicum decreased after 10 years of use in china. there are at least three problems, to our knowledge, making the finding suspicious or inconclusive in that report. in consideration of it as the first report about the emergence of potential artemisinin derivative-resistant s. japonicum to date and the possible severe infl ...	2011	21912960
[Anti-hepatofibrosis effect of fasudil hydrochloride on Schistosoma japonicum-infected mice].	To investigate the anti-fibrotic effect of fasudil hydrochloride on Schistosoma japonicum-infected mice, and the effect of fasudil hydrochloride on hepatic stellate cells (HSCs).	2011	21970106
[killing effect of sodium abietate on adult worms of schistosoma japonicum in vitro].	to observe the killing effect of sodium abietate on adult male and female worms of schistosoma japonicum in vitro.	2011	22164488
schistosoma japonicum ova maintains epithelial barrier function during experimental colitis.	to evaluate the impacts of schistosoma japonicum (s. japonicum) ova on the tight junction barriers in a trinitrobenzenesulfonic acid (tnbs)-induced colitis model.	2011	22147983
delayed tail loss during the invasion of mouse skin by cercariae of schistosoma japonicum.	summarya traditional assumption is that schistosome cercariae lose their tails at the onset of penetration. it has, however, recently been demonstrated that, for schistosoma mansoni, cercarial tails were not invariably being shed as penetration took place and a high proportion of tails entered human skin under experimental conditions. this phenomenon was termed delayed tail loss (dtl). in this paper, we report that dtl also happens with s. japonicum cercariae during penetration of mouse skin. it ...	2011	22018291
[study on manufacturing materials of imitation snails].	to make imitation snails by using substances with chemotaxis to schistosoma japonicum miracidia.	2011	22164382
a cleaved amplified polymorphic sequence (caps) method for the identification of geographical isolates of schistosoma japonicum in china.		2011	22185948
identification and characterization of six novel tetraspanins from schistosoma japonicum.	tetraspanins (tsps), also known as members of the trans-membrane 4 super-family (tm4sf), comprise an assemblage of surface antigens reported in eukaryotic organisms. in the work presented here, six novel tsp proteins from the human blood fluke schistosoma japonicum (s. japonicum) were produced and analyzed through a combination of bioinformatics and experimental approaches.	2011	21958506
[characterization and preliminary application of six monoclonal antibodies against recombinant signal protein 14-3-3 of schistosoma japonicum].	to identify the properties of six monoclonal antibodies (mcabs) against recombinant signal protein 14-3-3 of schistosoma japonicum, and investigate their value in the diagnosis of schistosomiasis.	2011	22164379
genetic manipulation of schistosomes - progress with integration competent vectors.	summarydraft genome sequences for schistosoma japonicum and s. mansoni are now available. the schistosome genome encodes ∼13 000 protein-encoding genes for which the functions of few are well understood. nonetheless, the new genes represent potential intervention targets, and molecular tools are being developed to determine their importance. over the past 15 years, noteworthy progress has been achieved towards development of tools for gene manipulation and transgenesis of schistosomes. a brief h ...	2011	21854676
Suppression of adaptive immunity to heterologous antigens by Sj16 of Schistosoma japonicum.	Abstract Despite great effort has been given to control the disease, schistosomiasis remains the most important human helminth infection in terms of morbidity and mortality. Natural infection of schistosomes induces very little protective immunity against reinfection. Moreover, effective schistosomiasis vaccines for practical use have not been developed. Schistosomes appear to have evolved highly effective modulatory mechanisms on their host's immune system that promote the parasites' survival ...	2011	22017401
[Screening interaction protein of Schistosoma japonicum gynecophoral canal protein by phage display].	To search the interaction protein of Schistosoma japonicum gynecophoral canal protein (SjGCP).	2011	22164491
Effectiveness of synthetic trioxolane OZ78 against Schistosoma japonicum in mice and rabbits.	Antischistosomal activities of a synthetic peroxide OZ78 (an ozonide carboxylic acid) against Schistosoma japonicum have been studied in mice and rabbits. Among 132 mice used, 30 of them were infected with 80-100 S. japonicum cercariae for collection of juvenile and adult schistosomes applied in in vitro tests. The remaining 102 mice were infected with 40 schistosome cercariae used for experimental treatment. Other 13 rabbits infected each with 200 schistosome cercariae were treated orally with ...	2011	22200956
neuroschistosomiasis: clinical symptoms and pathogenesis.	neuroschistosomiasis, referring to schistosomal involvement of the cns, when symptomatic, is a severe disorder in which prognosis depends largely on early diagnosis and treatment. it is an underdiagnosed disorder, but has been increasingly reported in populations in endemic areas and in tourists. cns involvement can occur at any time during schistosomal infection. both the brain and the spinal cord can be affected. schistosoma mansoni and schistosoma haematobium usually cause myelopathy, whereas ...	2011	21849166