Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the problem of plasmodium falciparum drug resistance in africa south of the sahara. | the resistance of p. falciparum malaria to dihydrofolate reductase inhibitors, to the 4-aminoquinolines (in particular, chloroquine), and to the combination sulfadoxine-pyrimethamine is reviewed in the light of past and the most recent findings. considering the gravity of the situation following the recent discovery of resistance of p. falciparum to chloroquine among the semi-immune populations of africa south of the sahara, a few suggestions are made for a realistic and rational approach to the ... | 1984 | 6397277 |
| the impact of malaria chemoprophylaxis on the immune status of africans. | the small number of studies undertaken so far indicates that malaria control with chemoprophylaxis depresses serum gamma-globulin levels and interferes with the development of the malaria antibodies detected by standard serological tests. whether these serological changes are associated with defective development of clinical immunity has yet to be clearly established. | 1984 | 6397278 |
| vector control operations in the african context. | in order to define the scope of vector control as a component of malaria control in the who african region, examples of recent experiences with different vector control methods in this region are reviewed. residual house spraying applied alone or in combination with mass drug administration has failed to interrupt malaria transmission in savanna areas for several technical and administrative reasons. nevertheless, there is evidence that residual house spraying has led to an improvement in genera ... | 1984 | 6397279 |
| vaccination against malaria: its plausibility and the present state of research. | a vaccine for public health use against malaria is urgently required and is being actively researched into. the present review outlines the biology of malaria parasites and the immune response to them, with an emphasis on the worst of the human diseases, and considers the current analytical and molecular biological work on malaria parasite surfaces and antigens. | 1984 | 6397929 |
| detection of antibodies to malaria: comparison of results with elisa, ifat, and crossed immunoelectrophoresis. | 194 sera from vietnamese refugees and 100 pairs of corresponding maternal and cord sera from liberia were examined for malaria-igg antibodies by enzyme-linked immunosorbent assay (elisa) and indirect immunofluorescence microscopy (ifat). for both tests the antigen was prepared from p. falciparum cultures. 44% of the vietnamese sera were positive in the elisa and 36% in the ifat, but results were discordant in 36% of cases. the liberian sera were all reactive in the ifat and 95% were reactive in ... | 1984 | 6397962 |
| the chemotherapy of rodent malaria, xxxviii. studies on the activity of three new antimalarials (wr 194,965, wr 228,258 and wr 225,448) against rodent and human malaria parasites (plasmodium berghei and p. falciparum). | in addition to their blood schizontocidal action on plasmodium berghei in vivo, two mannich bases wr 194,965 and 228,258 are also active against chloroquine-sensitive and chloroquine-resistant lines of p. falciparum in vitro. the response of the lines to each drug differs but shows no correlation in either case with response to chloroquine. the 8-aminoquinoline wr 225,448 is also active against p. falciparum in vitro but at much higher concentrations than the mannich bases. application of the 'c ... | 1984 | 6398032 |
| in vitro assessment of the antimalarial activity of chloroquine and its major metabolites. | a quantitative assessment of the antimalarial activity of chloroquine and its two major metabolites, desethylchloroquine (dec) and bis-desethylchloroquine (bis-dec), was made using a semi-automated microdilution technique. two isolates of plasmodium falciparum were used: the multi-drug resistant vietnam smith la 137 strain and the chloroquine-sensitive camp la 137 strain. mefloquine served as the standard for the vietnam smith strain and chloroquine for the camp strain. chloroquine and dec demon ... | 1984 | 6398033 |
| a malaria vaccine--necessary but not sufficient. | 1984 | 6398081 | |
| abnormal anti-single stranded (ss) dna activity in sera from plasmodium falciparum infected individuals. | sera from 32 subjects with plasmodium falciparum parasitaemia were screened for the presence of antibodies to native-double stranded (ds) dna and to heat-denaturated-single stranded (ss) dna by a farr dna binding radioimmunoassay. in addition anti-dsdna antibodies were also studied by indirect immunofluorescence using crithidia luciliae and rat liver sections as substrates. immunoglobulin (g, a, m) levels and plasmodial antibodies titres (pa) were concomitantly evaluated. the anti-ssdna activity ... | 1984 | 6398528 |
| the sensitivity of plasmodium falciparum to chloroquine and amodiaquine in ibadan, nigeria. | an extended in vivo test of the sensitivity of plasmodium falciparum to antimalarial drugs in nigerian children showed no evidence of resistance to chloroquine and amodiaquine. however, the results of a small number of in vitro tests suggest a decreased sensitivity of the parasite to chloroquine when compared with the results of earlier studies in the same locality. | 1984 | 6398531 |
| non-specific immunity to plasmodium falciparum: in vitro studies. | peripheral blood mononuclear cells (pbm) were cultured with plasmodium falciparum malaria for several cycles of parasite growth. non-immune pbm inhibited p. falciparum more frequently than immune pbm. the inhibiting factor appears to be released during the first 24 hours of culture and is a non-dialysable factor, probably originating from monocytes. the relevance of these findings to in vivo immunity is discussed. | 1984 | 6398533 |
| serum c-reactive protein levels and falciparum malaria. | a microplate elisa was developed to measure c-reactive protein (crp) and it was used to establish the relationship between crp levels and malaria. highest serum crp levels were found in african patients with high plasmodium falciparum parastaemia. however, even african children with lower parasitaemia had higher crp levels than others without parasitaemia. all african groups studied had crp levels above those of a control uk group. | 1984 | 6398534 |
| chloroquine resistant plasmodium falciparum malaria confirmed by in-vitro testing in a district hospital. | 1984 | 6398566 | |
| [evaluation of an alternative scheme of treatment for the control of malaria]. | 1984 | 6393363 | |
| [malaria and pregnancy]. | 1984 | 6393837 | |
| activities of respository preparations of cycloguanil pamoate and 4,4'-diacetyldiaminodiphenylsulfone, alone and in combination, against infections with plasmodium cynomolgi in rhesus monkeys. | the studies summarized in this report were concerned with the capacities of repository preparations of cycloguanil pamoate (cgt-p) to protect rhesus monkeys against infections with drug-susceptible and pyrimethamine-resistant strains of plasmodium cynomolgi. administered intramuscularly as a suspension in an oleaginous vehicle, cgt-p (i) provided long-term protection against single and repetitive challenges of rhesus monkeys with sporozoites of the drug-susceptible b and ro strains, (ii) effecte ... | 1984 | 6393864 |
| mechanism of pyrimethamine resistance in recent isolates of plasmodium falciparum. | clones of plasmodium falciparum prepared from recent isolates of infected blood were studied to determine the molecular mechanism of naturally occurring pyrimethamine resistance. total dna, as well as thymidylate synthetase and dihydrofolate reductase activities, were characterized from these lines. restriction analysis of dna from pyrimethamine-susceptible and -resistant lines of the parasite showed no obvious amplification of any dna fragment. further, analysis of dna from resistant and suscep ... | 1984 | 6393866 |
| chloroquine-resistant plasmodium falciparum malaria in zimbabwe. | 1984 | 6394141 | |
| [clinical observations on 196 cases of falciparum malaria in benin, west africa]. | 1984 | 6394168 | |
| [studies on ddt residual spraying for controlling anopheles lesteri anthropophagus and plasmodium falciparum malaria]. | 1984 | 6394169 | |
| [a misdiagnosed case of chloroquine-resistant falciparum malaria]. | 1984 | 6394171 | |
| [determination of the susceptibility of chloroquine-resistant plasmodium falciparum to pyronaridine]. | 1984 | 6394178 | |
| antibiotic sensitivity of plasmodium falciparum in vitro. | 1984 | 6394480 | |
| loss of resistance of plasmodium falciparum to chloroquine after culture adaptation. | 1984 | 6394481 | |
| a field test to assay pyrimethamine sensitivity of plasmodium falciparum in vitro. | 1984 | 6394482 | |
| epidemiological aspects of malaria in rameswaram island (tamil nadu). | 1984 | 6394483 | |
| plasmodium falciparum malaria and acute fulminant hepatitis. | 1984 | 6394586 | |
| [drug resistance of plasmodium falciparum and plasmodium vivax strains in cambodia (cardamone massif). morphological characteristics of plasmodium vivax]. | the authors report some failures of chemoprophylaxis and curative treatment of plasmodium falciparum and plasmodium vivax malaria. these infections were contracted in the area of the cambodian-thaï border by 8 europeans treated by various regimens and 34 cambodians treated by quinine. in the latter group, the quinine failure rate in plasmodium falciparum infections was about 47% (rii or riii?) and 17,5% in plasmodium vivax. total cure of vivax malaria with usual doses of primaquine among the eur ... | 1984 | 6394948 |
| malaria in christchurch. | thirty seven cases of malaria were diagnosed in the christchurch hospital microbiology laboratory from january 1978 to december 1983. cases occurred in most age groups with various occupations. prophylactic drugs may not give protection because of lack of patient compliance and/or parasite resistance. a case of malaria which recrudesced following treatment recommended in new zealand is also included. | 1984 | 6395073 |
| the effect of sulphadoxine/pyrimethamine on gametocytes in falciparum malaria. | a study of the effect of sulphadoxine/pyrimethamine (fansidar) on p. falciparum's gametocytes in peripheral blood was carried out in western thailand. one group of 77 patients with asexual form p. falciparum sensitive to fansidar were followed weekly to detect the appearance and the duration of gametocytes in peripheral blood after fansidar treatment on the basis of thick blood film examination. another group of 14 patients with sexual form p. falciparum was not given any antimalarial treatment ... | 1984 | 6395351 |
| comparative infectivity of knobless and knobby clones of plasmodium falciparum in splenectomized and intact aotus trivirgatus monkeys. | in two experiments, two knobless (k-) and two knob-producing (k+) clone-cultures of plasmodium falciparum, fcr-3/gambia strain, were injected into four aotus trivirgatus monkeys. the parasitemia in the k(-)-infected splenectomized (s-) monkey rose to a peak of 2.1% on the 16th day, while it reached only 0.7% at the same time in the k+ infected s- animal. passage from these animals (karyotype vi) into two intact (s+), naive monkeys of karyotype iii resulted in very light infections somewhat highe ... | 1984 | 6395532 |
| spontaneously released plasmodium falciparum merozoites from culture possess glycoproteins. | using metabolic labeling techniques with either radioactive glucosamine or various sugars in synchronous plasmodium falciparum cultures it was proven that merozoites isolated from such cultures possess glycoproteins. biosynthesis and processing of these glycoproteins were investigated. | 1984 | 6395533 |
| [pulmonary complications of malaria. apropos of 2 cases]. | 1984 | 6395601 | |
| the chemotherapy of rodent malaria xxxv. further studies on the retardation of drug resistance by the use of a triple combination of mefloquine, pyrimethamine and sulfadoxine in mice infected with p. berghei and 'p. berghei ns'. | in the face of an increasing prevalence of plasmodium falciparum resistant to chloroquine and to pyrimethamine-sulphonamide or -sulphone mixtures, the need for a new, effective blood schizontocide for treatment of acute malaria is urgent. the only such compound that is almost ready for release is mefloquine (m) but there is already a danger that parasites may become resistant to this compound if it is used extensively alone. earlier studies using a rodent model indicated that mefloquine could be ... | 1984 | 6395814 |
| erythrocyte invasion by malaria (plasmodium falciparum) merozoites: recent advances in the evaluation of receptor sites. | recent advances in the understanding of the cellular, biochemical and molecular aspects of the highly specific interactions between p. falciparum merozoites and human red blood cells are summarized. special attention is given to glycophorins a, b and c as receptors of invasion and to the role of carbohydrates and internal domains of glycophorin a, such as the wrb antigen, as receptor site. video pictures displaying the liberation of merozoites from schizont-infected red blood cells and the invas ... | 1984 | 6395849 |
| [sensitivity of plasmodium falciparum to chloroquine in the hyperendemic malaria savannah zone of burkina faso ex-upper volta. use of in vivo and in vitro tests. demonstration of a resistant strain in vitro]. | studies on the response of p. falciparum to chloroquine was conducted in schools located in the environs of koudougou (burkina faso, upper-volta), where malaria is meso to hyperendemic, using in vivo studies and in vitro macroculture tests. cases were obtained from school children, semi-immune asymptomatic carriers, in the 7-12 years-group. | 1984 | 6395988 |
| in vitro culture of plasmodium falciparum. | 1984 | 6396396 | |
| 2-acetylpyridine thiosemicarbazones xi: 2-(alpha-hydroxyacetyl)pyridine thiosemicarbazones as antimalarial and antibacterial agents. | a series of 2-(alpha-hydroxyacetyl)pyridine thiosemicarbazones was synthesized as potential antimalarial and antibacterial agents. their synthesis was achieved by the condensation of n4-mono- or n4,n4-disubstituted thiosemicarbazides with 2-(alpha-hydroxyacetyl)pyridine. the latter was prepared by selective bromine oxidation of (2-pyridinyl)-1,2-ethanediol. the new compounds show potent inhibitory activity against penicillin-sensitive as well as penicillin-resistant neisseria gonorrhoeae (mic, 0 ... | 1984 | 6396400 |
| biosynthesis of the target antigens of antibodies blocking transmission of plasmodium falciparum. | we have studied the biosynthesis of three proteins of mr 260 000, 59 000 and 53 000 previously identified on the surface of extracellular gametes of plasmodium falciparum as the targets of monoclonal antibodies which block infectivity of p. falciparum to mosquitoes. in cultures of p. falciparum pulse labeled with [35s]methionine we have found that these proteins are synthesized by gametocytes from an early stage in their maturation but are not synthesized by asexual blood stage parasites. the ta ... | 1984 | 6396517 |
| plasmodium falciparum merozoite vaccine in aotus monkeys. an evaluation of the tolerableness of three types of adjuvants. | eight aotus trivirgatus monkeys were included in a comparative study of adverse reactions caused by 3 types of malarial vaccine. the antigen consisted of late schizonts and merozoites combined with each of 3 adjuvants, a lipoidal amine cp-20,961, a muramyl dipeptide derivative [b30]-mdp, or freund's complete adjuvant (fca); 3 monkeys received cp-20,961 and 3 [b30]-mdp, whereas the fca vaccine was given to 2 animals. all the vaccine combinations caused some adverse reactions, but their nature and ... | 1984 | 6396835 |
| malaria chemoprophylaxis for multiple drug resistant plasmodium falciparum in africa: an increasing problem. | resistance of p. falciparum to the two groups of antimalaria drugs commonly used for prophylaxis, i.e. folate antagonists and 4-aminoquinolines has become a matter of great concern, also in africa. after 1978, p. falciparum developed resistance to the group of 4-aminoquinolines, which was first reported from east africa. chloroquine resistant infections are now spreading into central africa. similar developments are now appearing with the combination sulfadoxine/pyrimethamine. with the increasin ... | 1984 | 6396911 |
| [postoperative pernicious malarial attack in a subject, a native of an endemic zone]. | the case of an angolan woman who underwent, in her country, an hysterectomy requiring the transfusion of two whole blood units is reported. in the immediate postoperative period, acute renal failure with anuria set in, justifying the patient's transfer to an intensive care unit in france. upon admission, she had a thick blood film and serology work-up for plasmodium falciparum, both of which were negative. on the 20th day, the patient presented a pernicious malarial crisis with a concurrent rise ... | 1984 | 6388431 |
| clinical efficacy of sulfadoxine-pyrimethamine in the treatment of falciparum malaria in sabah, malaysia. | one hundred and ten consecutive patients with falciparum malaria were treated with fansidar and primaquine. of the 61 patients who were followed up at one week, 4 (6.6%) failed to clear their parasitaemia (1 r iii and 3 r ii treatment failures). of the subsequent 40 patients who were seen again at one month, another 3 (7.5%) had recrudesced (r i treatment failure). a total of 7 patients thus experienced some form of treatment failure in the cohort of 40 who completed the one month follow up. onl ... | 1984 | 6388486 |
| plasmodium falciparum: carbohydrates as receptor sites of invasion. | monosaccharides, disaccharides, and trisaccharides were tested as inhibitors of the in vitro growth of plasmodium falciparum (strain fcb). while certain monosaccharides (n-acetyl-d-glucosamine, d-mannose, and 3-o-methyl-d-glucose) proved to exhibit a toxic or reversibly retarding effect on the intraerythrocytic development of the parasite, the corresponding alpha- or beta-methylglycosides did not. several methylglycosides, synthetic di- and tri-saccharides, and artificial blood group antigens we ... | 1984 | 6389168 |
| a role for rheumatoid factor enhancement of plasmodium falciparum schizont inhibition in vitro. | studies were undertaken to determine whether rheumatoid factor (rf) was present in immune human and aotus trivirgatus monkey sera which inhibited plasmodium falciparum schizonts in vitro and to determine whether rf could be responsible for or contribute to merozoite agglutination in the parasite inhibition test. additional studies were conducted to determine the effect of exogenous rf on schizont inhibition when used alone or in conjunction with immune or normal sera. rf was not detected in any ... | 1984 | 6389357 |
| protein and nucleic acid synthesis during synchronized growth of plasmodium falciparum. | plasmodium falciparum, the human malarial parasite, was synchronized for asexual growth and pulse labeled to determine when rna, protein, and dna synthesis occurred. rna was synthesized during two periods in the 48-h developmental cycle, protein synthesis occurred throughout the cycle, and most dna was synthesized just before nuclear division (schizogony). | 1984 | 6389509 |
| clindamycin activity against chloroquine-resistant plasmodium falciparum. | the clindamycin dose-response curves observed with both chloroquine-resistant and chloroquine-susceptible strains of plasmodium falciparum in vitro demonstrated a plateau region that extended from 10(-2) to 10(1) micrograms/ml of drug (22 nm to 22 microm). similar dose-response curves were also observed with the three major metabolites of clindamycin (clindamycin sulfoxide, de-n-methyl clindamycin, and de-n-methyl clindamycin sulfoxide). the position of this plateau was time dependent and rose f ... | 1984 | 6389719 |
| effects of chloroquine on the feeding mechanism of the intraerythrocytic human malarial parasite plasmodium falciparum. | ultrastructural investigations of p. falciparum cultivated in vitro in human erythrocytes revealed new features of the feeding mechanism of the parasite. mature trophozoites and schizonts take up a portion of the host cytosol by endocytosis which is restricted to cytostomes and which involves the invagination of both parasitophorous and parasite membranes. the resulting endocytic vesicles, surrounded by two concentric membranes, migrate towards the central food vacuole membrane. the external mem ... | 1984 | 6389846 |
| the effects of aspirin-containing serum in the continuous culture of plasmodium falciparum. | in vitro culture of plasmodium falciparum-infected human erythrocytes (rbc) has permitted systematic study of human host-parasite relations. in this study the effect of aspirin in the culture system was examined by using serum from blood of fasting, healthy male volunteers, before and after the ingestion of aspirin. the addition of aspirin-containing serum disturbed parasite growth and development: 0-1/2 dilutions of treated/control sera inhibited parasite development, with nuclear pyknosis, pyk ... | 1984 | 6389847 |
| effects of mitochondrial protein synthesis inhibitors on the incorporation of isoleucine into plasmodium falciparum in vitro. | plasmodium falciparum was grown in human erythrocytes in vitro and the effect of chloramphenicol, erythromycin, and tetracycline on growth and maturation of the parasites and on their ability to incorporate [3h]isoleucine into protein was observed. exposure of rings to high concentrations of chloramphenicol had little effect on subsequent maturation of the rings whereas brief (4 h) exposure of trophozoites caused a dose-dependent inhibition of subsequent ring formation. incorporation of [3h]isol ... | 1984 | 6389849 |
| stimulation of autoantibody production in normal blood lymphocytes by malaria culture supernatants. | supernatants from plasmodium falciparum cultures containing soluble parasite material were mitogenic for normal human peripheral blood mononuclear cells (mnc) in vitro. this was evidenced by blast transformation and significant incorporation of 3h-thymidine and confirms earlier reports of the mitogenic potential of malaria parasites. lymphocyte activation by these malaria derived products was polyclonal as demonstrated by increased secretion of iga, igg and igm by the stimulated cells. using rat ... | 1984 | 6390302 |
| erythrocyte membrane receptor for plasmodium falciparum. | 1984 | 6390453 | |
| receptor mediated endocytosis of the malarial parasite by erythrocytes. | malarial merozoites appear to interact with the major surface sialoglycoproteins, glycophorin a and glycophorin b, during entry into the human erythrocyte. intact merozoites bind 125i-glycophorin demonstrating directly that the parasites have glycophorin binding sites. a glycophorin containing fraction crosslinked to acrylamide beads provided a probe to identify merozoite molecules with an affinity for the erythrocyte surface. a fraction of merozoites, labeled with 3h-proline, and containing the ... | 1984 | 6390454 |
| kenyan chloroquin, fansidar and quninine resistant p. falciparum. | 1984 | 6386173 | |
| [imported malaria in brest hospitals from 1974 to 1980]. | from 1974 to 1980, 42 confirmed hospital cases of imported malaria occurred in brest. for 4 cases, we have no information. for the others, 24 care europeans and 14 non-europeans. among the europeans, the merchant-seamen are the most exposed group. a high proportion of infections are due to plasmodium falciparum, mainly from black africa. one patient had a neuropaludism and three had serious attacks. in about half of patients, the initial diagnosis was not malaria. all cases of p. falciparum were ... | 1984 | 6386204 |
| malaria in urban and rural areas of southern ghana: a survey of parasitaemia, antibodies, and antimalarial practices. | a comparative cross-sectional survey was undertaken in two populations, urban and rural, in southern ghana to assess the impact of urbanization on the prevalence of malaria parasitaemia and antibodies. at the same time, a survey of antimalarial practices was conducted on sample populations in the two communities. the results showed a low parasite rate (1.6%) and correspondingly low titres of malaria antibodies in a significant proportion of the urban community, particularly in children less than ... | 1984 | 6386208 |
| a new in vitro test for pyrimethamine/sulfadoxine susceptibility of plasmodium falciparum and its correlation with in vivo resistance in kenya. | a useful in vitro method for field evaluation of plasmodium falciparum sensitivity to pyrimethamine/sulfadoxine is described. thirty-five kenyan schoolchildren infected with p. falciparum were treated with this drug combination and followed up for 5 weeks. in vitro tests for sensitivity to these drugs and to chloroquine were performed before starting treatment. all infections cleared within 7 days of treatment, but 5 children had recurrent parasitaemia within 35 days. the original isolates from ... | 1984 | 6386209 |
| plasmodium falciparum in haiti: susceptibility to pyrimethamine and sulfadoxine-pyrimethamine. | eighteen patients with plasmodium falciparum infection were studied in port-au-prince, haiti, to monitor the response of the malaria parasite to sulfadoxine-pyrimethamine. in all infections the parasitaemia was cleared rapidly following treatment with standard dose of the drug combination; no recrudescence was observed during follow-up periods of 1 week (4 patients) and 4 weeks (14 patients). parallel in vitro tests indicated that 5 of the 16 isolates successfully tested were resistant to pyrime ... | 1984 | 6386210 |
| a comparative micro in-vitro test of p. falciparum to mefloquine and chloroquine in gadchiroli district, maharashtra state. | 1984 | 6386964 | |
| isolation of artemisinin (qinghaosu) from artemisia annua growing in the united states. | 1984 | 6387056 | |
| [importance of night monkeys (aotus) in research on malaria chemotherapy]. | 1984 | 6387820 | |
| complications of malaria. | 1984 | 6388081 | |
| [response of human plasmodium to antimalarials on the island of saint louis, state of maranhão, brazil]. | 1984 | 6390656 | |
| malaria research. | 1984 | 6390682 | |
| plasmodium falciparum infection in bisazir chemosterilized anopheles dirus in laboratory studies. | bisazir, at a 0.5% solution induced sterility in males and at a 1.5% solution in female a. dirus. these sterilizing doses reduced p. falciparum infection in mosquitoes, however, they can still transmit malaria. it is concluded that by the concentration of 1.5 and 2.0% that induced complete sterility in males and females are not safe in sterile-male release programme for the control of a. dirus, unless all females were eliminated prior to release. | 1984 | 6390705 |
| vector incrimination studies and observations on species a and b of the taxon anopheles culicifacies in pakistan. | studies on anopheline mosquitoes in selected villages of punjab province, pakistan have incriminated anopheles culicifacies species a as the primary malaria vector. although an. stephensi and an. subpictus showed higher immediate gut infection rates, estimations of relative abundance, age structure and survivorship, and observations of late gut and salivary gland infection rates suggested that neither species was a major vector in these villages. a survey of an. culicifacies populations detected ... | 1984 | 6390803 |
| use of human plasma for continuous in vitro cultivation of plasmodium falciparum. | plasma from units of human blood collected in cpda-1 preservative were compared with human serum in cultures of plasmodium falciparum. no significant differences in parasite growth and multiplication were seen between cultures containing serum or plasma. the wider availability of plasma makes it an attractive alternative to serum, especially in large scale cultures. | 1984 | 6390805 |
| altered red cell membrane fluidity during schizogonic development of malarial parasites (plasmodium falciparum and p. lophurae). | the plasma membranes of human or duckling erythrocytes infected with malarial parasites (plasmodium falciparum and p. lophurae respectively) were stained by the fluorescent dye merocyanine 540 in the presence of serum. unparasitized erythrocytes from infected ducklings or from in vitro cultures remained unstained in the presence of serum. because merocyanine 540 has a greater affinity for fluid phased or disordered lipid bilayers the results suggest that upon infection of the red blood cell the ... | 1984 | 6390806 |
| indirect elisa test for malaria in blood donors. | to determine the potential risk of transfusion malaria at the hospital militar central in bogota, colombia, sera from 3114 blood donors were tested for malaria antibodies by the indirect elisa technique. positive results were found in 8.6 per thousand of the serum samples using p. falciparum antigen containing more than 60% mature forms as substrate. three cases of transfusion-induced malaria were confirmed during the study. the first patient developed a p. vivax infection one week after the adm ... | 1984 | 6390807 |
| african serum interference in the determination of chloroquine sensitivity in plasmodium falciparum. | isolates of plasmodium falciparum from villagers in central sudan were tested for chloroquine and mefloquine sensitivity using the who microtechnique procedure and a modified 48-h in vitro test for drug resistance. no drug-resistant strains were noted. in the who procedure, in which parasites were cultivated in the presence of the patient's plasma, 72% of the isolates failed to mature to the schizont stage, but when infected erythrocytes were washed free of the patient's plasma and cultivated in ... | 1984 | 6391006 |
| imported malaria in japan: hematological aspects. | 1984 | 6391064 | |
| gametocyte production in cloned lines of plasmodium falciparum. | gametocyte production by cloned lines of plasmodium falciparum and their parental isolates has been studied in culture over periods of several months. many isolates differed significantly from each other in their capacity for gametocyte production. clones derived from an individual isolate were also widely different in capacity for gametocyte production. consistent differences in gametocyte production were observed between clones which had always been grown concurrently and thus had identical cu ... | 1984 | 6391217 |
| monoclonal antibody characterization of plasmodium falciparum antigens. | we characterized a set of eight monoclonal antibodies produced against plasmodium falciparum. in an indirect fluorescent antibody assay the antibodies produced small dots of fluorescence in schizonts and individual merozoites. this merozoite-associated dot reactivity occurred with 21 different strains of p. falciparum, but there was no reactivity with other human, nonhuman primate, or rodent plasmodium species. three of the monoclonal antibodies precipitated proteins of mr 145,000, 135,000, and ... | 1984 | 6391218 |
| proteins responsible for a punctate fluorescence pattern in plasmodium falciparum merozoites. | mouse monoclonal antibodies (mcabs) have been used to characterize the proteins of the asexual erythrocytic cycle of plasmodium falciparum. three different mcabs react with antigens of the schizont and extracellular merozoite to give a punctate fluorescence pattern. in many cases, such areas of fluorescence were composed of two adjacent, fluorescent bodies; these were distinct from the nuclei. in contrast, mcabs which bound to the ring-stage parasite were not localized, but were diffusely distri ... | 1984 | 6391219 |
| [obtaining hepatic stages of plasmodium falciparum in vitro]. | sporozoites of plasmodium falciparum, obtained by membrane feeding of anopheles freeborni or a. stephensi with cultured gametocytes, were used to infect monolayers of human hepatocytes. fluorescent labelling with an african serum as well as giemsa staining performed from day one to day 7 of cultures, demonstrated the presence of numerous hepatic schizonts measuring up to 40 micron. | 1984 | 6391340 |
| [epidemiology of malaria in tanna (vanuatu)]. | a malaria survey done on the island of tanna (southern part of vanuatu) showed that this island was hypoendemic with a few areas mesoendemicity where permanent breeding site are available for anopheles farauti. the average spleen rate was 5,25% and the parasite rate 10,43%. plasmodium vivax was the most prevalent species. the vector anopheles farauti was found to be endophilic. recently, chloroquin resistance was observed. | 1984 | 6391709 |
| [p. falciparum malaria contracted in burundi, resistant to chloroquine and diminished sensitivity to quinine]. | the authors report a case of malaria from burundi, due to chloroquine resistant plasmodium falciparum. it is the second case of chloroquine resistance, confirmed by in vitro tests, in this country. decreased sensitivity to quinine is demonstrated by in vivo study, and quinine assay. the isolate was sensitive to amodiaquine and mefloquine in vitro. | 1984 | 6391710 |
| [anti-i anti-erythrocyte auto-immunization in malaria]. | an anti-erythrocyte autoimmunization with anti-i specificity was shown in patients previously exposed to malaria (falciparum malaria in 98.7% of cases). an anti-i antibody was eluted from red cells in 14.6% of 41 patients with an acute p. falciparum infection. the frequency of the anti-i immunisation was found to be correlated to the rate of the antimalarial antibodies among the 152 patients without parasitaemia, coming from areas endemic for falciparum malaria: this frequency was 5.2% for a rat ... | 1984 | 6391711 |
| recent progress in the development of malaria vaccines: memorandum from a who meeting. | the sixth meeting of the scientific working group on the immunology of malaria reviewed studies on the identification and analysis of malarial antigens of asexual blood stages and sexual stages (gametes, zygotes, ookinetes) that may be exploited as targets for vaccination. several proteins have been identified on the surface of mature schizonts and free merozoites, some of which can be recognized by antibodies which block in vitro parasite growth. immunization of rodents and monkeys with purifie ... | 1984 | 6391715 |
| malaria control with residual fenitrothion in central java, indonesia: an operational-scale trial using both full and selective coverage treatments. | an operational-scale trial, using residual fenitrothion, for control of malaria was carried out in central java, indonesia, from 1980 to 1982. two areas, each comprising about 70 km(2) and a population of about 50 000, were treated with fenitrothion (40% water dispersible powder) at a target dosage of 2 g/m(2) for 3 cycles at 6-monthly intervals. one area was treated with full coverage (i.e., the interiors of houses and cattle shelters were sprayed to a height of 3 m) for 2 cycles, followed by a ... | 1984 | 6391717 |
| identification of the acidic compartment of plasmodium falciparum-infected human erythrocytes as the target of the antimalarial drug chloroquine. | chloroquine (cq), the most widely used antimalarial drug, is an acidotropic agent (de duve, 1983) which accumulates to high levels in malaria-infected erythrocytes. a possible site of accumulation of the drug, the parasite's food vacuole, has been implicated in the mode of action of cq. we have defined the various compartments of plasmodium falciparum-parasitized human erythrocytes in terms of their ph and capacity to accumulate bases. the host cell and the parasite cytosols were differentially ... | 1984 | 6391917 |
| the present state of malaria research: an historical survey. | 1984 | 6391946 | |
| malaria: immunity, vaccination and immunodiagnosis. | 1984 | 6391948 | |
| new answers through chemotherapy? | 1984 | 6391949 | |
| epidemiological and entomological features of an outbreak of fever in meghraj block, sabarkantha district, gujarat state (india) in 1980. | 1984 | 6392409 | |
| malignant malaria in saudi arabia. | 1984 | 6392440 | |
| initial extracellular development in vitro of merozoites of plasmodium falciparum. | late schizonts from continuous cultures of p. falciparum were concentrated over percoll, inoculated to various experimental media at the rate of about 20 x 10(6) per 0.5 ml of medium, and incubated in a candle jar at 37 degrees for 1 day. controls in standard culture medium showed a heavy invasion with young rings in the previously uninfected red cells introduced with the inoculum of schizonts. in a medium of high potassium content containing a 33% extract of human erythrocytes, this invasion wa ... | 1984 | 6392532 |
| [treatment of malaria. update]. | 1984 | 6392768 | |
| [present status of malaria]. | 1984 | 6392798 | |
| a reevaluation of the status of cholesterol in erythrocytes infected by plasmodium knowlesi and p. falciparum. | the cholesterol synthesis of rhesus monkey erythrocytes parasitized by plasmodium knowlesi and human erythrocytes infected by p. falciparum, as measured by incorporation of [1-14c]acetate and 3h2o, was almost undetectable, concordant with very low levels of measurable 3-hydroxy-3-methyl glutaryl-coa reductase activity. in addition, both types of infected cells exchanged cholesterol with the plasma at the same rate as uninfected cells. the data do not exclude the possibility of cholesterol transf ... | 1984 | 6392884 |
| [complicated course of tropical malaria in the harbor hospital; survey of 1970-1981]. | 1984 | 6392908 | |
| [preferential action of chloroquine on plasmodium within mature erythrocytes]. | in vitro studies on the effects of chloroquine on plasmodium berghei in relation to the age of the host cell made it possible to demonstrate the preferential effect of the drug on parasites growing in mature red blood cells. the ed-50 for parasites in mature red blood cells is lower (1,64 +/- 0,2 mg/kg/day) than in reticulocytes (2,45 +/- 0,2 mg/kg/day). the result of clumping test for chloroquino-sensitive p. berghei growing in young red blood cells, is almost similar as in a chloroquino-resist ... | 1984 | 6393008 |
| human antibodies to a mr 155,000 plasmodium falciparum antigen efficiently inhibit merozoite invasion. | igg from a donor clinically immune to plasmodium falciparum malaria strongly inhibited reinvasion in vitro of human erythrocytes by the parasite. when added to monolayers of glutaraldehyde-fixed and air-dried erythrocytes infected with the parasite, this igg also displayed a characteristic immunofluorescence restricted to the surface of infected erythrocytes. elution of the igg adsorbed to such monolayers gave an antibody fraction that was 40 times more efficient in the reinvasion inhibition ass ... | 1984 | 6393131 |
| a review of chloroquine resistance by plasmodium falciparum in kenya. | 1984 | 6381026 | |
| monoclonal antibody identifies circumsporozoite protein of plasmodium malariae and detects a common epitope on plasmodium brasilianum sporozoites. | we produced a hybridoma secreting an immunoglobulin g1 monoclonal antibody against the circumsporozoite protein of the human malaria parasite plasmodium malariae (uganda 1/cdc). the monoclonal antibody produces a circumsporozoite precipitation reaction when incubated with viable sporozoites of p. malariae and reacts at high titers with heat-fixed sporozoites in an indirect immunofluorescent antibody test. using the purified monoclonal antibody and western blot analysis, we identified two polypep ... | 1984 | 6381308 |
| a molecular basis for strain specificity in s antigens of plasmodium falciparum. | 1984 | 6381590 | |
| antimalarial immunity in saimiri monkeys. immunization with surface components of asexual blood stages. | plasmodium falciparum polypeptides of 200 and 140 k mol wt exposed at the surface of merozoites and/or schizonts were purified by affinity chromatography and by electroelution from sodium dodecyl sulfate-polyacrylamide gel electrophoresis. monkeys were separated into three groups of four and immunized either with one of the two polypeptides or with saline (control). after intravenous challenge with 2.5 x 10(7) p. falciparum asexual blood stages, two monkeys of the control group had to be treated ... | 1984 | 6381635 |
| the three major antigens on the surface of plasmodium falciparum merozoites are derived from a single high molecular weight precursor. | a 195,000 mol wt plasmodium falciparum protein and processing fragments derived from it have been purified by monoclonal antibody affinity chromatography. a polyvalent antiserum has been raised against the purified protein and used to identify the terminal processing products associated with the merozoite. three unique fragments of 83,000, 42,000, and 19,000 mol wt are present and they represent the major surface antigens of p. falciparum merozoites. | 1984 | 6381636 |
| effects of neoglycoproteins on penetration of plasmodium falciparum merozoites into erythrocytes in vitro. | sugars conjugated to bovine serum albumin (neoglycoproteins) were tested for their ability to inhibit plasmodium falciparum merozoite invasion of red blood cells in an in vitro inhibition assay. the inhibitory effects of the neoglycoproteins on merozoite invasion of erythrocytes were determined by assessing 3h-hypoxanthine incorporation into the cultures. the only neoglycoprotein that caused significant inhibition n-acetyl-glucosamine-bovine serum albumin. a comparison of the inhibitory effects ... | 1984 | 6381683 |
| immune sera recognize on erythrocytes plasmodium falciparum antigen composed of repeated amino acid sequences. | protective immune responses against the asexual stages of the human malaria parasite, plasmodium falciparum, are most probably directed against exposed antigenic determinants on the surface of the free merozoite or the infected red blood cell, and therefore antigens in these locations are candidates for testing as components of a defined molecular vaccine. to facilitate the search for such antigens, we recently developed a method for the expression of p. falciparum proteins in escherichia coli a ... | 1984 | 6382025 |