Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| a randomized trial of intravaginal nonoxynol 9 versus oral metronidazole in the treatment of vaginal trichomoniasis. | this study was undertaken to investigate the efficacy of nonoxynol 9 suppositories in the treatment of vaginal trichomoniasis. | 2000 | 10819810 |
| incidence and predictors of reinfection with trichomonas vaginalis in hiv-infected women. | the presence of sexually transmitted infections (stis) may facilitate transmission of hiv to uninfected partners. | 2000 | 10821602 |
| evolution of the eukaryotic translation termination system: origins of release factors. | accurate translation termination is essential for cell viability. in eukaryotes, this process is strictly maintained by two proteins, eukaryotic release factor 1 (erf1), which recognizes all stop codons and hydrolyzes peptidyl-trna, and eukaryotic release factor 3 (erf3), which is an elongation factor 1alpha (ef-1alpha) homolog stimulating erf1 activity. to retrace the evolution of this core system, we cloned and sequenced the erf3 genes from trichomonas vaginalis (parabasalia) and giardia lambl ... | 2000 | 10833194 |
| trichomonas vaginalis in urine cytology. | 2000 | 10834019 | |
| the length of the combined 3' untranslated region and poly(a) tail does not control rates of glyceraldehyde-3-phosphate dehydrogenase mrna translation in three species of parasitic protists. | experimental observations suggested that the length of the glyceraldehyde-3-phosphate dehydrogenase (gapdh) mrna 3' end has a role in regulating rates of translation in the parasitic protists trypanosoma brucei, leishmania donovani, and trichomonas vaginalis. using a pcr assay for poly(a) tail length, we measured the size of the rna 3' end under different growth conditions in all three species. our results showed that the combined 3' untranslated region and poly(a) tail of gapdh mrna do not vary ... | 2000 | 10852893 |
| cytopathogenic effect of trichomonas vaginalis on human vaginal epithelial cells cultured in vitro. | in this study we established human vaginal epithelial cells (hvecs) in culture and evaluated their interaction with trichomonas vaginalis parasites to complement previous studies using other cell types. primary cultures of hvecs were established. contaminating fibroblasts were separated from epithelial cells by differential trypsinization. specific antibody staining revealed that over 92% of cells in hvec monolayers were epithelial cells. t. vaginalis adhered to hvecs and produced severe cytotox ... | 2000 | 10858237 |
| host and tissue specificity of trichomonas vaginalis is not mediated by its known adhesion proteins. | adhesion of trichomonas vaginalis is believed to be dependent on four adhesion proteins, which are thought to bind to vaginal epithelial cells in a specific manner with a ligand-receptor type of interaction. however, the specific receptors on the host cell have not yet been identified. in this work, the ability of the t. vaginalis adhesins to bind to cells of different histologic derivations and from different species has been studied. hela, cho, and vero cell lines; erythrocytes from different ... | 2000 | 10858260 |
| trichomonas vaginalis: characterization, expression, and phylogenetic analysis of a carbamate kinase gene sequence. | the gene encoding carbamate kinase (cbk, atp:carbamate phosphotransferase, ec 2.7.2.2) from trichomonas vaginalis has been sequenced and its expression in this protozoon has been verified using reverse-transcription polymerase chain reaction. the codon usage and percentage nucleotide composition in the coding and noncoding regions are consistent with other genes isolated from this parasite. phylogenetic analysis of this gene has suggested possible speciation events that are congruent with other ... | 2000 | 10864518 |
| etiology of sexually transmitted infections among street-based female sex workers in dhaka, bangladesh. | an etiological study of sexually transmitted infections (stis) was conducted among female sex workers (fsws) in dhaka, bangladesh. endocervical swab and blood samples from 269 street-based fsws were examined for neisseria gonorrhoeae, chlamydia trachomatis, and trichomonas vaginalis as well as for antibodies to treponema pallidum and herpes simplex virus 2 (hsv-2). sociodemographic data and data regarding behavior were also collected. a total of 226 of the 269 fsws (84%) were positive for the st ... | 2000 | 10699032 |
| failure to detect dna in hydrogenosomes of trichomonas vaginalis by nick translation and immunomicroscopy. | 2000 | 10699261 | |
| [incidence of genitourinary infection caused by chlamydia trachomatis in a std center calculated by direct antigen detection]. | chlamydia trachomatis is one of the most common sexually transmitted agents which causes a wide spectrum of diseases including urethritis in men and endocervicitis in women. we analyzed patients with genitourinary c. trachomatis infections evaluating risk factors and the association with other sexually transmitted infections. | 2000 | 11153203 |
| antimycotic activity and phagocytosis effects of econazole in combination with ibuprofen isobuthanolammonium against vaginal strains. | vaginal infections caused by candida spp., other yeasts and trichomonas vaginalis are problematic mainly due to the various factors involved in development of infection and to the failure of common treatments. in this study we investigated the presence of synergistic activity of econazole and ibuprofen isobuthanolammonium against 310 different vaginal isolates, by using the microdilution broth assay to test in vitro antimicrobial activity and the effect of the two drugs on phagocytosis and intra ... | 2000 | 11154035 |
| the use of molecular techniques for the diagnosis and epidemiologic study of sexually transmitted infections. | molecular diagnostic tests are more sensitive and, in many cases, more specific than conventional laboratory methods for the detection of sexually transmitted infections. here, we review recently developed molecular methods for the diagnosis and subtyping of the most common sexually transmitted infections: infections caused by chlamydia trachomatis, neisseria gonorrhoeae, human papillomavirus, trichomonas vaginalis, and the agents of genital ulcer disease (haemophilus ducreyi, herpes simplex vir ... | 2000 | 11095835 |
| phylogenomic analysis of the alpha proteasome gene family from early-diverging eukaryotes. | we employed a phylogenomic approach to study the evolution of alpha subunits of the proteasome gene family from early diverging eukaryotes. blast similarity searches of the giardia lamblia genome identified all seven alpha proteasome genes characteristic of eukaryotes from the crown group. in addition, a pcr strategy for the amplification of multiple alpha subunit sequences generated single alpha proteasome products for representatives of the kinetoplastida (leishmania major), the parabasalia (t ... | 2000 | 11116327 |
| a randomized, placebo-controlled trial of monthly azithromycin prophylaxis to prevent sexually transmitted infections and hiv-1 in kenyan sex workers: study design and baseline findings. | our objectives were to describe the baseline findings of a trial of antibiotic prophylaxis to prevent sexually transmitted infections (stis) and hiv-1 in a cohort of nairobi female sex workers (fsws). a questionnaire was administered and a medical examination was performed. hiv-negative women were randomly assigned to either one gram azithromycin or placebo monthly. mean age of the 318 women was 32 years, mean duration of sex work 7 years and mean number of clients was 4 per day. high-risk behav ... | 2000 | 11138916 |
| high prevalence of trichomoniasis in rural men in mwanza, tanzania: results from a population based study. | to measure the prevalence of urethral infections including trichomoniasis in rural tanzanian men, to assess the prevalence of symptoms and signs among men with trichomonas vaginalis, and to analyse the risk factors for trichomoniasis. | 2000 | 11141851 |
| systematic review of diagnostic tests for vaginal trichomoniasis. | to review critically and to summarize the evidence of diagnostic tests and culture media for the diagnosis of trichomonas vaginitis. | 2000 | 11220487 |
| molecular typing of trichomonas vaginalis isolates by hsp70 restriction fragment length polymorphism. | subtyping isolates of trichomonas vaginalis is an essential tool for understanding the epidemiology of this common sexually-transmitted disease. restriction fragment length polymorphism (rflp) analysis employing a probe from the heat-inducible cytoplasmic hsp70 gene family hybridized with ecor i-digested genomic dna was used in the molecular typing of trichomonas isolates. analysis of five american type culture collection (atcc) reference strains and 31 jackson, mississippi, isolates from six ma ... | 2000 | 11220758 |
| baseline std prevalence in a community intervention trial of the female condom in kenya. | we present baseline sexually transmitted disease (std) prevalence rates from an ongoing intervention trial at kenyan agricultural sites. | 2000 | 11221128 |
| iron hydrogenases and the evolution of anaerobic eukaryotes. | hydrogenases, oxygen-sensitive enzymes that can make hydrogen gas, are key to the function of hydrogen-producing organelles (hydrogenosomes), which occur in anaerobic protozoa scattered throughout the eukaryotic tree. hydrogenases also play a central role in the hydrogen and syntrophic hypotheses for eukaryogenesis. here, we show that sequences related to iron-only hydrogenases ([fe] hydrogenases) are more widely distributed among eukaryotes than reports of hydrogen production have suggested. ge ... | 2000 | 11070057 |
| lateral gene transfer and metabolic adaptation in the human parasite trichomonas vaginalis. | 2000 | 11070064 | |
| levels of vaginal secretory leukocyte protease inhibitor are decreased in women with lower reproductive tract infections. | secretory leukocyte protease inhibitor contributes resistance to primary human immunodeficiency virus infection in the oral cavity. however, the levels of this inhibitor in the genital tract of women with sexually transmitted diseases or vaginitis are not well described. the objective was to determine vaginal inhibitor levels in women with symptomatic and asymptomatic genital infections. | 2000 | 11084573 |
| pyridazine n-oxides. iii. synthesis and "in vitro" antimicrobial properties of n-oxide derivatives based on tricyclic indeno[2,1-c]pyridazine and benzo[f]cinnoline systems. | a number of 9h-indeno[2,1-c]pyridazine n-oxides (3a-c) and benzo[f]cinnoline n-oxides (4,5a-c) have been synthesized and tested for antimicrobial activity. all new products were inactive against gram negative bacteria and fungi. in contrast, among the compounds synthesized, 3b, 4b and 5b showed a moderate activity against gram positive staphylococcus aureus and staphylococcus epidermidis. of the present series, the 9-nitro-benzo[f]cinnoline n-oxide 5b possessed the highest activity especially ag ... | 2000 | 11092137 |
| 18s ribosomal dna-based pcr for diagnosis of trichomonas vaginalis. | trichomonas vaginalis remains the most common sexually transmitted parasite in the world and is considered a major risk factor in the transmission of the human immunodeficiency virus. a pcr technique using primers targeting a specific region of the 18s rrna gene of t. vaginalis was developed. the pcr test was standardized using 15 reference strains, giving a single product of 312 bp in all strains. no amplification was observed when dna from related organisms or human dna was used as a target. t ... | 2000 | 10878064 |
| incidence of gardnerella vaginalis, candida sp and human papilloma virus in cytological smears. | in spite of the wide-ranging literature on the microbiology of normal and abnormal flora of the vagina, there are few studies on the relationship between human papilloma virus (hpv) and other vaginal microorganisms. | 2000 | 10887386 |
| in vitro screening of american plant extracts on trypanosoma cruzi and trichomonas vaginalis. | from the beginning of this decade and with the revival of the phytotherapy, biological research about immunomodulatory, anti-inflammatory and antiprotozoal effects of central and south american plants have been in progress. our objective was to determine the antiprotozoal activity of 79 extracts from different plant families, including asteraceae, araceae, moraceae, solanaceae, rhamnaceae, zingiberaceae, leguminosae and sapotaceae. once matching with herbarium specimens authenticated the plants, ... | 2000 | 10904152 |
| prevalence of sexually transmitted infections among clients of female commercial sex workers in thailand. | clients of commercial sex workers are considered at high risk for the acquisition and transmission of sexually transmitted diseases (stds). identification and treatment of infections in this group could help to reduce the transmission of stds. | 2000 | 10907913 |
| interventions for treating trichomoniasis in women. | around 120 million women worldwide suffer from trichomonas vaginalis vaginitis every year. the infection is sexually transmitted and is believed to facilitate hiv transmission. | 2000 | 10908466 |
| trichomonas vaginalis and bacterial vaginosis. coexistence in vaginal wet mount preparations from pregnant women. | to identify how frequently trichomoniasis and characteristics of bacterial vaginosis (bv) occur concomitantly in wet mount preparations from pregnant women. | 2000 | 10710744 |
| presence of a member of the mitochondrial carrier family in hydrogenosomes: conservation of membrane-targeting pathways between hydrogenosomes and mitochondria. | a number of microaerophilic eukaryotes lack mitochondria but possess another organelle involved in energy metabolism, the hydrogenosome. limited phylogenetic analyses of nuclear genes support a common origin for these two organelles. we have identified a protein of the mitochondrial carrier family in the hydrogenosome of trichomonas vaginalis and have shown that this protein, hmp31, is phylogenetically related to the mitochondrial adp-atp carrier (aac). we demonstrate that the hydrogenosomal aac ... | 2000 | 10713172 |
| competition and protease sensitivity assays provide evidence for the existence of a hydrogenosomal protein import machinery in trichomonas vaginalis. | hydrogenosomes are double membrane bounded redox organelles found in a number of amitochondriate protists and fungi. they are involved in carbohydrate metabolism and atp synthesis and thus resemble mitochondria. molecular analysis of the hydrogenosomal heat shock proteins hsp70, hsp60 and hsp10 in trichomonas vaginalis, one of the deepest-branching eukaryotes known to date, has revealed that these group exclusively with mitochondrial heat shock proteins. this finding indicates strongly that a pr ... | 2000 | 10743607 |
| centrin protein and genes in trichomonas vaginalis and close relatives. | anti-centrin monoclonal antibodies 20h5 and 11b2 produced against clamydomononas centrin decorated the group of basal bodies as well as very closely attached structures in all trichomonads studied and in the devescovinids foaina and devescovina. moreover, these antibodies decorated the undulating membrane in trichomonas vaginalis, trichomitus batrachorum, and tritrichomonas foetus, and the cresta in foaina. centrin was not demonstrated in the dividing spindle and paradesmosis. immunogold labelin ... | 2000 | 10750840 |
| gynaecological infections as risk determinants of subsequent cervical neoplasia. | a longitudinal cohort study was carried out to determine whether gynaecological infections other than human papillomavirus (hpv) are also related to the subsequent increased risk of cervical neoplasia. the study comprised 19114 women attending the organized mass screening in finland in 1985-1990 with cytologically detected hpv, actinomyces, herpes simplex, trichomonas vaginalis, or yeast. the women were followed-up for subsequent preinvasive lesions and invasive cancers until the end of 1994 by ... | 2000 | 10752657 |
| epidemiologic issues of sexually transmitted diseases in sexual assault victims. | the objective of this study was to investigate the epidemiologic aspects of sexually transmitted diseases (stds) in victims of sexual assault and to discuss the methodological issues in determining risk of std acquisition. we performed a comprehensive review of the medical literature to determine the prevalence of stds in victims of sexual assault. a medline search and a search of bibliographies of published manuscripts was performed to discover relevant articles published in the english languag ... | 2000 | 10639679 |
| resistance of trichomonas vaginalis to metronidazole: report of the first three cases from finland and optimization of in vitro susceptibility testing under various oxygen concentrations. | trichomonas vaginalis is a globally common sexually transmitted human parasite. many strains of t. vaginalis from around the world have been described to be resistant to the current drug of choice, metronidazole. however, only a few cases of metronidazole resistance have been reported from europe. the resistant strains cause prolonged infections which are difficult to treat. t. vaginalis infection also increases the risk for human immunodeficiency virus transmission. we present a practical metho ... | 2000 | 10655382 |
| the complete cdna sequence of a type ii trichomonas vaginalis virus. | trichomonas vaginalis viruses (tvv), which may regulate p270 gene expression in the protozoan pathogen t. vaginalis, are a group of divergent double-stranded (ds) rna viruses. in the present study, the complete 4674-bp cdna sequence of a 4.6-kb ds rna from a newly identified tvv2-1 isolate was determined. the sequence of the plus-strand mrna contains four open reading frames, which encode overlapping cap and pol genes in the reading frame 2 and reading frame 1, respectively, and two putative ser ... | 2000 | 10662630 |
| disease prevalence in women attending the std clinic in mumbai (formerly bombay), india. | our objectives were to determine the prevalence of neisseria gonorrhoeae and its association with other std causing organisms. three hundred and thirty-six consecutive women (female sex workers (fsws) and married contacts), attending a sexually transmitted disease (std) clinic in mumbai, were screened for n. gonorrhoeae, chlamydia trachomatis and trichomonas vaginalis. per speculum examination was performed and clinical signs were recorded. symptoms perceived by the women were also recorded. the ... | 2000 | 10667900 |
| immunolocalization of two hydrogenosomal enzymes of trichomonas vaginalis. | three monoclonal antibodies specific for malic enzyme and for the alpha- and beta-subunits, respectively, of the succinyl-coenzyme a (coa) synthetase of trichomonas vaginalis were used to immunolocalize these proteins in the cell. all antibodies labeled the hydrogenosome matrix as determined both by immunofluorescence and by immunogold staining. there was no labeling on the cell surface or in any other cell compartment. these results support the idea that these proteins are restricted to a hydro ... | 2000 | 10669133 |
| trichomonas vaginalis: detection of nucleoside hydrolase activity as a potential screening procedure. | 2000 | 10673349 | |
| effect of chlorhexidine on genital microflora, neisseria gonorrhoeae, and trichomonas vaginalis in vitro. | chlorhexidine is a disinfectant that has been used in skin and mouth washes and as a preservative in some vaginal lubricants. a gel containing 0.25% chlorhexidine gluconate has been found to be effective against chlamydia trachomatis in vitro and in animal models. applied vaginally, 5 g of this gel could achieve vaginal fluid concentrations of < or = 1250 microg/ml. | 2000 | 10676973 |
| the impact of regular vaginal ph screening on the diagnosis of bacterial vaginosis in pregnancy. | bacterial vaginosis has recently been associated with preterm labor and delivery. the purpose of our study was to determine whether regular prenatal vaginal ph testing resulted in more frequent diagnoses of bacterial vaginosis and other vaginal infections, more frequent treatment with antibiotics, and fewer preterm deliveries. we also sought to determine the sensitivity and specificity of ph testing and vaginal symptom reporting in identifying vaginal infections. | 2000 | 10678339 |
| antigenicity of trichomonas vaginalis heat-shock proteins in human infections. | patients infected with trichomonas vaginalis mount humoral and cellular immune responses that often do not protect against reinfection. the oxidative stressors produced by leukocytes may trigger a heat-shock-like response in t. vaginalis trophozoites, helping the parasite to survive host immune defenses. the antigenicity of t. vaginalis heat-shock proteins (hsps) was examined by immunoprecipitation of t. vaginalis heat-induced proteins with sera from infected patients and controls. when t. vagin ... | 2000 | 10685842 |
| biology of trichomonosis. | trichomonas vaginalis is emerging as a major pathogen of men and women and is associated with serious health consequences. advances in diagnosis and treatment are presented. the complexity of trichomonad pathogenesis is illustrated in the interaction of this parasite with human cells, tissues and the immune system. it is now becoming evident that the interaction of trichomonads with the host is frequently modulated by environmental signals. the molecular biology of trichomonads is still in its i ... | 2000 | 11964771 |
| [the technical improvement of staining of trichomonas vaginalis]. | 2000 | 12206005 | |
| use of intravaginal microbicides to prevent acquisition of trichomonas vaginalis infection in lactobacillus-pretreated, estrogenized young mice. | d2a21, a novel peptide antibiotic has in vitro activity against a wide spectrum of sexually transmitted diseases (std). in this study we tested the hypothesis that intravaginal d2a21 would interfere with acquisition of trichomonas vaginalis infection in a modified mouse model. t. vaginalis infections of estrogenized young mice pretreated with lactobacillus vaginalis or lactobacillus rhamnosus were more frequent and persistent than those in mice pre-treated with lactobacillus gasseri or lactobaci ... | 2000 | 11421379 |
| trichomonas vaginalis: chromatin and mitotic spindle during mitosis. | the mitotic phases and the changes that the chromatin and mitotic microtubules undergo during mitosis in the sexually transmitted parasite trichomonas vaginalis are described. parasites arrested in the gap 2 phase of the cell cycle by nutrient starvation were induced to mitosis by addition of fresh whole medium. [(3)h] thymidine labeling of trichomonad parasites for 24 h showed that parasites have at least four synchronic duplications after mitosis induction. fixed or live and acridine orange (a ... | 2000 | 11162363 |
| effect of the antimicrobial peptide, d-hecate, on trichomonads. | tritrichomonas foetus and trichomonas vaginalis are protozoan parasites that cause sexually transmitted diseases in cattle and humans, respectively. there is a need for new antimicrobial agents to treat or prevent trichomoniasis because there are currently no approved chemotherapeutic agents against t. foetus and resistance of t. vaginalis to metronidazole does occur. therefore, we evaluated the effect of a novel antimicrobial peptide, d-hecate, on the viability of 6 isolates of t. foetus and t. ... | 2000 | 11191917 |
| a novel chemotherapeutic combination for trichomonas vaginalis targeting purine salvage pathways of the parasite. | a novel combination of chemotherapy (dipyridamole/allopurinol) targeting the possible purine salvage mechanisms of trichomonas vaginalis was used in this study. the possible effect of these drugs either individually or in combination, on the multiplication of t. vaginalis in modified tym axenic culture, was assessed on 24 hours intervals. the combination of both drugs at concentrations of 5 microm and 10 microm each showed a potent lethal effect on the parasite and an almost complete inhibition ... | 2000 | 11198371 |
| diagnostic value of the papanicolaou smear in lower genital tract infections during pregnancy. | to evaluate the diagnostic value of the pap smear in lower genital tract infections during pregnancy. | 2000 | 25951154 |
| trichomonas vaginalis-an indicator for other sexually transmitted infecting agents. | the present study is based on 350 women having sexually transmitted diseases (std) and 68 male counterparts. trichomonas vaginalis was a significant contributor in 216 (61.7%) out of 350 female std cases and 56 (82.3%) out of 68 male counterparts. further, out of 126 (58.3%) out of 216 cases of t. vaginalis, 41 cases (32.5%) were associated with candida species; 29 cases (23%) were associated with neisseria gonorrhoeae (n gonorrhoeae); haemophilus ducreyi (h. ducreyi) 18 cases (14.3%) and chlamy ... | 2000 | 20877088 |
| validity of the papanicolaou smear in the diagnosis of candida spp., trichomonas vaginalis, and bacterial vaginosis. | to determine the validity of the papanicolaou smear in the diagnosis of bacterial vaginosis, trichomonas vaginalis, and candida spp. | 2001 | 17050980 |
| epidemiology of sexually transmitted diseases in río cuarto, argentina. | sexually transmitted diseases (std) are a group of transmittable diseases acquired fundamentally through sexual contact. std are a social problem resulting from demographic explosion and changes in sexual conduct, which affects teenagers and adults of all socioeconomic strata. the goal of this work was to establish the actual state of the different std within the studied population. samples of vaginal fluids, endocervical materials and urethral exudates taken from 2,630 patients during five year ... | 2001 | 17061502 |
| trichomonas vaginalis in turkey--a review. | 2001 | 16897944 | |
| [prevalence of trichomonas vaginalis donné in women of lódź population in 1955-1999 years]. | search for trichomonas vaginalis was carried out in direct (in 0.85% nacl and 0.1% safranine) and fixed stained microscopical preparations ( wright or giemsa methods, after fixation with 70% methyl alcohol) and cultures ( pavlova, johanson-trussel-john, roiron-ratner, simic media), from materials of the vagina, urethra and cervix uteri. in the years 1955-1999 a total of about 12000 women were examined. the prevalence of t. vaginalis in women population of lódź region was high (from 26.6% still 7 ... | 2001 | 16897945 |
| [hydrolytic activity of trichomonas vaginalis donné strains]. | the hydrolytic activity of trichomonas vaginalis donné strains was investigated using api zym test (biomérieux). among 19 determined hydrolases were found alkaline and acid phosphatases; also in several strains n-acetyl-beta-glucosaminidase and a-glucosidase were demonstrated. the api zym test may be used for characterization of enzymatic activity of t. vaginalis strains, but adequate medium is necessary. | 2001 | 16897946 |
| [application of an alternative method to counting in neubauer chamber to determine trichomonas vaginalis concentrations]. | it was demonstrated that there is a relation of cell concentration with the parasite reading in an elisa reader. it was determined that absorbance showed significant values in wavelengths of visible range and the possible minimum wavelength (450 nm)was chosen to guarantee the maximum sensitivity. likewise, it was confirmed that absorbance significantly increased (p < 0.001) after the plate was filled up, it stabilized in the 40m-2h time interval when applying 100, 150, 200 or 300 ml per well of ... | 2001 | 15846921 |
| risk factors for vaginal trichomoniasis among women in basra, iraq. | we investigated trichomonas vaginalis infection among 352 women with vaginal discharge, 46 were found to be infected, an infection rate of 13%. there were no significant differences in the isolation rate of t. vaginalis in women according to occupation, educational level, economic status, age, marital status, parity, menstrual status and contraception use. the difference in the isolation rates of t. vaginalis in women with a history of abortion (7.6%) and in women with no history of abortion (15 ... | 2001 | 15332733 |
| diterpenoids from azorella yareta and their trichomonicidal activities. | diterpenoids with trichomonicidal activity were isolated from the aerial parts of azorella yareta hauman. one was 13beta-hydroxyazorellane, together with the known constituents mulinolic acid, mulin-11,13-dien-20-oic acid, azorellanol and 13alpha-hydroxyazorellane. their structures were determined by spectroscopic and chemical methods. | 2001 | 11219811 |
| curable sexually transmitted infections among the clientele of a family planning clinic in istanbul, turkey. | integrating screening and treatment of sexually transmitted infections into existing family planning programs can be a good way to fight this problem. also, the number of studies on the prevalence of certain sexually transmitted infections in this population in turkey is limited. | 2001 | 11196049 |
| clinical impact of identifying trichomonas vaginalis on cervicovaginal (papanicolaou) smears. | the purpose of this study was to understand how clinicians manage asymptomatic women after trichomonas has been reported on papanicolaou (pap) smears. clinical information was obtained from questionnaires sent to healthcare providers whenever trichomonas was identified during the study period. trichomonas was identified in 173 (1.4%) of 12,547 pap smears examined. completed questionnaires were returned on 95 (55%) patients, and 92 patients were included in this study. sixty-three (68%) patients ... | 2001 | 11241904 |
| etiology of urethral discharge in west africa: the role of mycoplasma genitalium and trichomonas vaginalis. | to determine the etiological role of pathogens other than neisseria gonorrhoeae and chlamydia trachomatis in urethral discharge in west african men. | 2001 | 11242818 |
| evolutionary relationships of the glucokinase from the amitochondriate protist, trichomonas vaginalis. | two genes coding for trichomonas vaginalis glucokinase were isolated and sequenced. the putative translation products have molecular masses of 41,584 and 41,772 da, corresponding to 375 and 377 amino acids, respectively. these values agree with data determined by sodium dodecylsulfate-polyacrylamide gel electrophoresis (sds-page) for the enzyme purified from the organism. the two sequences showed 78% amino acid identity. the sequences and their phylogenetic reconstruction show that they are memb ... | 2001 | 11250082 |
| [sexual abuse in children: prevention of sexually transmitted diseases]. | when a child suffers from sexual abuse clinical guidelines must be established. there is a risk of infection from the following agents responsible for sexually transmitted diseases: the hepatitis b, hepatitis c and human immunodeficiency viruses, neisseria gonorrhoeae, chlamydia trachomatis, syphilis, herpes simplex virus, bacterial vaginosis, papillomavirus, trichomonas vaginalis and pediculus pubis. therefore, a follow-up with periodic serological monitoring for 1year and immunoprophylaxis or ... | 2001 | 11262256 |
| bacterial vaginosis among a group of married jordanian women: occurrence and laboratory diagnosis. | a total of 310 vaginal swabs collected from a group of married jordanian women complaining of vaginal discharge were examined for bacterial vaginosis. the scoring system of nugent for the interpretation of gram staining was employed. this system revealed the presence of the condition in 29.7% of patients. results obtained using the scoring system correlated significantly with the detection of clue cells and the scarcity of white blood cells in the vaginal discharge. an inverse relationship was f ... | 2001 | 11368266 |
| evaluation of genetic damage induced by a nitroimidazole derivative in human lymphocytes: tinidazole (tnz). | one of the useful drugs in the treatment against infestations caused by trichomonas vaginalis, entamoeba histolytica and giardia lamblia is tinidazole (tnz) 1-[2-(ethylsulfonyl) ethyl]-2-methyl-5-nitroimidazole) (gilman r.h., marquis g.s., miranda e., vestegui m., martinez h., 1988. rapid reinfection by giardia lamblia after treatment in a hyperendemic third world community. lancet i, 343-345). we decided to evaluate the potential genetic damage induced by tnz using different biological biomarke ... | 2001 | 11377093 |
| self-obtained vaginal swabs for diagnosis of treatable sexually transmitted diseases in adolescent girls. | to ascertain the acceptability of testing and prevalence of 3 readily treatable sexually transmitted diseases (stds) (infections with neisseria gonorrhoeae, chlamydia trachomatis, and trichomonas vaginalis) with the use of patient-obtained vaginal swabs. | 2001 | 11386956 |
| phagocytosis of erythrocytes by trichomonas vaginalis: examination of a cervicovaginal smear. | 2001 | 11391827 | |
| molecular evidence for a fe-hydrogenase in the green alga scenedesmus obliquus. | fe-hydrogenase-specific degenerate primers were used in polymerase chain reactions with single-stranded scenedesmus obliquus cdna as template. a resulting 435-bp fragment was used to screen a cdna library from s. obliquus. a 1.6-kb cdna clone, containing the 3'-end of an open reading frame, was obtained. its deduced amino acid sequence contains the conserved h-cluster motives unique for fe-hydrogenases. any other fes-clusters seem to be absent. phylogenetically, the enzyme is closely related to ... | 2001 | 11400057 |
| a mobile phone text message and trichomonas vaginalis. | 2001 | 11402245 | |
| self-collection of vaginal swabs for the detection of chlamydia, gonorrhea, and trichomoniasis: opportunity to encourage sexually transmitted disease testing among adolescents. | many sexually transmitted diseases (stds) are prevalent among adolescents, yet compliance to undergo std testing by this population is suboptimal. efforts to enhance compliance with testing among at-risk youth are needed. | 2001 | 11403188 |
| occurrence of trichomoniasis in women in denmark, 1967-1997. | the neisseria unit at statens serum institut (ssi), copenhagen, denmark administers laboratory surveillance systems for gonorrhea and chlamydia, however, no similar system has been available for trichomoniasis. | 2001 | 11403189 |
| characterisation of an atp diphosphohydrolase (apyrase, ec 3.6.1.5) activity in trichomonas vaginalis. | in the present report the enzymatic properties of an atp diphosphohydrolase (apyrase, ec 3.6.1.5) in trichomonas vaginalis were determined. the enzyme hydrolyses purine and pyrimidine nucleoside 5'-di- and 5'-triphosphates in an optimum ph range of 6.0--8.0. it is ca(2+)-dependent and is insensitive to classical atpase inhibitors, such as ouabain (1 mm), n-ethylmaleimide (0.1 mm), orthovanadate (0.1 mm) and sodium azide (5 mm). a significant inhibition of adp hydrolysis (37%) was observed in the ... | 2001 | 11403767 |
| antiprotozoal activity of neurolaena lobata. | extracts, fractions and sesquiterpene lactones from neurolaena lobata (l.) r. br. (asteraceae), a traditional medicinal plant from guatemala, were tested in vitro against leishmania spp. promastigotes, trypanosoma cruzi trypomastigotes and epimastigotes and trichomonas vaginalis trophozoites. the ethanol extract inhibited the parasite growth of l. mexicana, t. cruzi and t. vaginalis significantly. the pure germacranolides 1 and a mixture of 2 and 3, isolated from the ethonal extract, were highly ... | 2001 | 11406857 |
| construction and bootstrap analysis of dna fingerprinting-based phylogenetic trees with the freeware program freetree: application to trichomonad parasites. | the win95/98/nt program freetree for computation of distance matrices and construction of phylogenetic or phenetic trees on the basis of random amplified polymorphic dna (rapd), rflp and allozyme data is presented. in contrast to other similar software, the program freetree (available at http://www.natur.cuni.cz/~flegr/programs/freetree or http://ijs.sgmjournals.org/content/vol51/issue3/) can also assess the robustness of the tree topology by bootstrap, jackknife or operational taxonomic unit-ja ... | 2001 | 11411692 |
| unusual diversity in alpha-amanitin sensitivity of rna polymerases in trichomonads. | previous studies in the parasitic protist trichomonas vaginalis have revealed that protein coding genes are transcribed by an alpha-amanitin-resistant rna polymerase (rnap) ii. to investigate whether this unusual property is a general characteristic of trichomonads, we addressed the physiology of rna synthesis in lysolecithin-permeabilized cells. unlike in t. vaginalis, rnap ii in tritrichomonas foetus was highly sensitive to the inhibitor alpha-amanitin. on the other hand, rnap iii, identified ... | 2001 | 11420110 |
| sexually transmitted infections and cervicovaginal dysplasia in a cohort of human immunodeficiency virus-positive women in turin. | the correlation between sexually transmitted infections and cervicovaginal dysplasia has been evaluated in a cohort of 135 women who tested positive for human immunodeficiency virus type i (hiv-1) and were admitted to amedeo di savoia hospital of turin during the years 1997 and 1998 (stages b2 and b3 or c2 and c3). of these women. 31 presented with sexually transmitted diseases (stds; mean age, 33.5 +/- 5.9 years). among them, 14 were affected by cervicovaginal dysplasia of differing severity; h ... | 2001 | 11270419 |
| mycoplasma hominis and trichomonas vaginalis symbiosis: multiplicity of infection and transmissibility of m. hominis to human cells. | we recently reported that most trichomonas vaginalis isolates cultured in vitro are infected by mycoplasma hominis. in this work, we have characterized some aspects of the relationships between the two microorganisms. pcr, cultivation, and immunological methods revealed that the number of m. hominis organisms carried by t. vaginalis in culture varied from isolate to isolate, suggesting a specific multiplicity of infection. moreover, infected t. vaginalis isolates were able to pass bacteria not o ... | 2001 | 11271423 |
| loss of multiple hydrogenosomal proteins associated with organelle metabolism and high-level drug resistance in trichomonads. | land, k. m., clemens, d. l., and johnson, p. j. 2001. loss of multiple hydrogenosomal proteins associated with organelle metabolism and high-level drug resistance in trichomonads. experimental parasitology 97, 102-110. in trichomonads, metronidazole is activated to its cytotoxic form in a specialized energy-producing organelle called the hydrogenosome. electron transport components in the organelle, pyruvate:ferredoxin oxidoreductase and ferredoxin, donate a single electron to the drug, converti ... | 2001 | 11281707 |
| sexually transmitted infections among married women in dhaka, bangladesh: unexpected high prevalence of herpes simplex type 2 infection. | to document the prevalence of reproductive tract infections (rti) and sexually transmitted infections (sti) among women attending a basic healthcare clinic in dhaka, bangladesh, to identify risk factors associated with the diseases and to estimate the incidence of syphilis, hepatitis c (hcv), hepatitis b (hbv), and herpes simplex type 2 (hsv-2) infection. | 2001 | 11287690 |
| aetiology of urethral discharge in bangui, central african republic. | to determine the aetiology of urethritis in bangui, central african republic. | 2001 | 11287692 |
| hydrogenosome morphological variation induced by fibronectin and other drugs in trichomonas vaginalis and tritrichomonas foetus. | the hydrogenosome is a spherical organelle, found in some anaerobic protozoa, which participates in atp and molecular hydrogen formation. the morphological alterations in hydrogenosomes induced by fibronectin, hydroxyurea and cytochalasin b in trichomonas vaginalis and tritrichomonas foetus are presented. we demonstrate that, under experimental conditions, the hydrogenosome presents a high diversity in size and shape, suggesting a mechanism that seems to compensate for the stress provoked by dru ... | 2001 | 11293569 |
| enzyme immunoassay for urogenital trichomoniasis as a marker of unsafe sexual behaviour. | enzyme immunoassay (eia) was used to detect antibodies to trichomonas vaginalis in sera from zimbabwe. the eia showed a sensitivity of 95 and 94% when compared with vaginal swab culture among women attending a family planning clinic (fpc) and female commercial sex workers (csw) respectively. the specificity was 85 and 77% in the two groups. culture-negative fpc women were sub-divided into high risk or low risk of exposure to trichomoniasis. the seroprevalence was 10% (6/61) among low risk women, ... | 2001 | 11293668 |
| host and pathogen interaction during vaginal infection by trichomonas vaginalis and mycoplasma hominis or ureaplasma urealyticum. | vaginal infections by trichomonas vaginalis and mycoplasma hominis have been shown to be associated. since m. hominis and ureaplasma urealyticum are similar pathogens, both belonging to the class of the mycoplasmata, we describe here a molecular study into the interdependence of u. urealyticum and t. vaginalis during infection. susceptibility towards infection by u. urealyticum depends on genetic polymorphism in the interleukin-1 receptor antagonist (il-1ra) gene. now, we defined the relation be ... | 2001 | 11295198 |
| antimicrobial activity of omalanthus nervosus. | the ethanol extracts of omalanthus nervosus leaves, stem and root barks, were partitioned (petrol, dichloromethane, ethyl acetate). in respect to crude extracts, all fractions demonstrated broader spectrum and higher levels of antibacterial activity, ethyl acetate fractions being in all cases the more active. no fraction was active against tested moulds. | 2001 | 11295305 |
| antimicrobial activity of harpullia ramiflora. | the methanol extracts of harpullia ramiflora, leaves, flowers, stem and root barks were partitioned (petrol, dichloromethane, ethyl acetate). in respect to crude extracts, all fractions demonstrated broader spectrum and higher levels of antibacterial activity. the ethyl acetate fraction of the flowers exhibited the highest activity. no activity was shown against the tested moulds. | 2001 | 11295311 |
| predictors of the vaginal microflora. | our purpose was to define influences on the patterns of the vaginal microflora. | 2001 | 11303191 |
| antibiotic prophylaxis to prevent post-abortal upper genital tract infection in women with bacterial vaginosis: randomised controlled trial. | to determine the prevalence of bacterial vaginosis in women undergoing first trimester suction termination of pregnancy and to evaluate the efficacy of metronidazole in reducing the risk of post abortal pelvic infection in women with bacterial vaginosis. | 2001 | 11305547 |
| acidic glycerol lipids of trichomonas vaginalis and tritrichomonas foetus. | the isolation and characterization of acidic lipids from both trichomonas vaginalis and tritrichomonas foetus have been carried out using radiolabeling, a combination of high performance liquid and thin layer chromatographic techniques, and mass spectrometry. unique among the eukaryotes, these organisms produce phosphatidylglycerols and o-acyl phosphatidylglycerol-like compounds. in this study, the molecular weight distributions of the phosphatidylglycerols and acyl phosphatidylglycerols were de ... | 2001 | 11308025 |
| nitroimidazole-resistant vaginal trichomoniasis treated with paromomycin. | a 33-year old woman with nitroimidazole-resistant vaginal trichomoniasis is described. she was treated with intravaginal paromomycin (500 mg daily for 2 days). this cured the trichomoniasis but resulted in severe local side effects. paromomycin may be useful for difficult cases of nitroimidazole-resistant trichomonas vaginalis vaginitis. the exact dosage still has to be determined. | 2001 | 11311775 |
| purification of n-acetyl-beta-d-hexosaminidase from trichomonas vaginalis. | 2001 | 11312579 | |
| effect of iron on the virulence of trichomonas vaginalis. | the role of iron was evaluated with respect to the virulence of trichomonas vaginalis in mice. iron-supplemented and iron-depleted diamond's trypticase-yeast extract-maltose (tym) media were prepared by adding 360 microm of ferrous sulfate and 100 microm of 2,2'-dipyridyl. trophozoites cultivated from normal tym and iron-supplemented tym media produced subcutaneous abscesses; however, trichomonads grown in an iron-deficient tym medium failed to produce any pathology. in addition to the increased ... | 2001 | 11318588 |
| the burden of reproductive-organ disease in rural women in the gambia, west africa. | data on the epidemiology of reproductive-organ morbidity are needed to guide effective interventions, to set health-care priorities, and to target future research. this study aimed to find out the prevalence of reproductive-organ disease in a sample of rural gambian women. | 2001 | 11323043 |
| [microbiologic profile in symptomatic pregnant women's genital secretions in gran buenos aires, argentina]. | establish the prevalence of microorganisms associated with genital colonization in symptomatic pregnant women. in order to review the evolution of frequent pathogens ecology and adjust the laboratory design, in a population attended at the public health hospital, in the great buenos aires. | 2001 | 11333586 |
| [trichomonas vaginalis: treatment and resistance to nitroimidazoles]. | 2001 | 11333589 | |
| trichomonas vaginalis has two fibronectin-like iron-regulated genes. | trichomonas vaginalis, a protozoan parasite of the human urogenital tract, interacts with fibronectin (fn), a glycoprotein of the extracellular matrix. we, therefore, attempted to identify genes of this eukaryote encoding fn-binding proteins. | 2001 | 11343805 |
| an evaluation of infectious diseases in cervicovaginal smears from patients with atypical cells of undetermined significance. | despite all studies of infectious disease of the female genital tract, there have been a few studies of the many different types of infectious organisms on pap smears that contain abnormal cells. the purpose of this study is to evaluate the significance of infectious organisms in women with a cytologic diagnosis of atypical squamous cells of undetermined significance (ascus). this study utilizes 398 cervicovaginal smears and concurrent cervical biopsies (test and control cases) obtained from the ... | 2001 | 11347382 |
| trifluoromethionine, a prodrug designed against methionine gamma-lyase-containing pathogens, has efficacy in vitro and in vivo against trichomonas vaginalis. | methionine gamma-lyase, the enzyme which catalyzes the single-step conversion of methionine to alpha-ketobutyrate, ammonia, and methanethiol, is highly active in many anaerobic pathogenic microorganisms but has no counterpart in mammals. this study tested the hypothesis that this pathogen-specific enzyme can be exploited as a drug target by prodrugs that are exclusively activated by it. trifluoromethionine was confirmed as such a prodrug and shown to be highly toxic in vitro to the anaerobic pro ... | 2001 | 11353620 |
| drug susceptibility testing of anaerobic protozoa. | a simple technique for routine, reproducible global surveillance of the drug susceptibility status of the anaerobic protozoa trichomonas, entamoeba, and giardia is described. data collected using this technique can be readily compared among different laboratories and with previously reported data. the technique employs a commercially available sachet and bag system to generate a low-oxygen environment and log(2) drug dilutions in microtiter plates, which can be monitored without aerobic exposure ... | 2001 | 11353630 |
| sexual behavior among adolescent women at high risk for sexually transmitted infections. | the temporal pattern of partners and sexual encounters may be key factors in the acquisition and transmission of sexually transmitted diseases (stds). behavior among adolescent women is of particular interest because they frequently have the highest prevalence and incidence of infection. | 2001 | 11354261 |
| enzymes on microbial pathogens and trichomonas vaginalis: molecular mimicry and functional diversity. | 2001 | 11422079 |