Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| mating attraction by stenotus rubrovittatus (heteroptera: miridae) females and its relationship to ovarian development. | stenotus rubrovittatus (matsumura) (heteroptera: miridae) causes brown or black marks on rice, oryza sativa l., grains (pecky rice), and it is becoming of increasing importance in japan. attractiveness of adult females or males of s. rubrovittatus to conspecific individuals was examined in the field in 2003 and 2004. unmated female-baited traps captured significantly more males than did the unmated male-baited traps. however, the numbers of females captured by female- or male-baited traps were l ... | 2007 | 17849880 |
| proteomic analysis of rice (oryza sativa) seeds during germination. | although seed germination is a major subject in plant physiological research, there is still a long way to go to elucidate the mechanism of seed germination. recently, functional genomic strategies have been applied to study the germination of plant seeds. here, we conducted a proteomic analysis of seed germination in rice (oryza sativa indica cv. 9311) - a model monocot. comparison of 2-de maps showed that there were 148 proteins displayed differently in the germination process of rice seeds. a ... | 2007 | 17849412 |
| gene transcription in the leaves of rice undergoing salt-induced morphological changes (oryza sativa l.). | we describe the gene expression profile of third leaves of rice (cv. nipponbare) seedlings subjected to salt stress (130 mm nacl). transcripts of mn-sod, cu/zn-sod,cytosolic and stromal apx, gr and catb were regulated, whereas expression of thylakoid-bound apx and cata were down-regulated. the levels of the compatible solute proline and of transcripts of its biosynthetic gene, delta1-pyrroline-5-carboxylate synthetase (p5cs), were strongly increased by salt stress. interestingly, a potential com ... | 2007 | 17846498 |
| [the differential expression of the genes of the key enzymes involved in phenolic compound metabolism in rice (oryza sativa l.) under different nitrogen supply]. | differential expression of the key genes controlling phenolic metabolism in allelopathic and non-allelopathic rice accessions was investigated under two nitrogen supply levels (lower and normal) using fluorescence quantitative-polymerase chain reaction (fq-pcr) (figs.2, 3). the results indicated that 9 key enzyme genes concerned were mediated by lower nitrogen level (table 2). all of the nine genes (table 1, fig.4), were up-regulated by 1.9-5.4 times of the relative gene expression amounts in al ... | 2007 | 17960041 |
| mutation in nicotianamine aminotransferase stimulated the fe(ii) acquisition system and led to iron accumulation in rice. | higher plants acquire iron (fe) from the rhizosphere through two strategies. strategy ii, employed by graminaceous plants, involves secretion of phytosiderophores (e.g. deoxymugineic acid in rice [oryza sativa]) by roots to solubilize fe(iii) in soil. in addition to taking up fe in the form of fe(iii)-phytosiderophore, rice also possesses the strategy i-like system that may absorb fe(ii) directly. through mutant screening, we isolated a rice mutant that could not grow with fe(iii)-citrate as the ... | 2007 | 17951455 |
| the phylogeny and expression pattern of apetala2-like genes in rice. | the multigene families undergo birth-and-death evolution and thus contribute to biological innovations. the apetala2-like genes belong to the euap2 group of the ap2 gene family. these genes are characterized by several distinct motifs and exist in ferns, gymnosperms, and angiosperms. the phylogenetic analysis indicated that these genes have undergone the birth-and-death evolution. the five apetala2-like genes in rice (oryza sativa l.) display redundant but distinct expression patterns as demonst ... | 2007 | 17945171 |
| genotypic difference in silicon uptake and expression of silicon transporter genes in rice. | rice (oryza sativa) is a highly silicon (si)-accumulating species that shows genotypic differences in si accumulation. we investigated the physiological and molecular mechanisms involved in the genotypic difference in si uptake between the japonica var. nipponbare and the indica var. kasalath. both the si concentration in the shoot and the si uptake per root dry weight were higher in nipponbare than in kasalath grown in either soil or nutrient solution. the si uptake by a single root was also hi ... | 2007 | 17905867 |
| study the effect of nitrogen fertilizer management on dry matter remobilization of three cultivars of rice (oryza sativa l.). | in order to study the effect of nitrogen fertilizer management on dry matter remobilization among three rice cultivars, a field experiment was carried out at the rice research institute of iran-deputy of mazandaran (amol). in 2005, a split-split experiment in the basis of randomized complete block design with 3 replications and 3 factors were used in which fertilizer rate (100, 150 and 200 kg ha(-1) from urea source), fertilizer split application (3 levels in variable ratios in transplanting, ti ... | 2007 | 19090163 |
| rice tillering dwarf mutant dwarf3 has increased leaf longevity during darkness-induced senescence or hydrogen peroxide-induced cell death. | senescence or cell death in plant leaves is known to be inducible by darkness or h(2)o(2). when the arabidopsis gene max2/ore9 is disrupted, leaf senescence or cell death in response to the above stimuli is delayed. because the rice (oryza sativa l.) gene dwarf3 (d3) is orthologous to max2/ore9, we wished to know whether disruption of d3 also results in increased longevity in leaves. we found that darkness-induced senescence or h(2)o(2)-induced cell death in the third leaf [as measured by chloro ... | 2007 | 17895586 |
| different patterns of genealogical relationships found in the two major qtls causing reduction of seed shattering during rice domestication. | the three quantitative trait loci (qsh1, qsh3, and qsh4) causing reduction of seed shattering were investigated to examine their relative importance during rice domestication. the qsh1 and qsh4 loci showed a distinct effect on the reduction of shattering, compared with the qsh3 locus. fine mapping and sequence analysis strongly suggested that the qsh1 and qsh4 loci are the same as the recently reported genes. a non-shattering allele at qsh1 drastically changed the shattering phenotype to a non-s ... | 2007 | 17893735 |
| genetic analysis and fine mapping of a dynamic rolled leaf gene, rl10(t), in rice (oryza sativa l.). | a dynamically rolled leaf mutant (rl10) was identified from a spontaneous mutation in an oryza sativa l. subsp. indica line, ii-32b. the leaf chlorophyll content of rl10 is higher than that of the wild type. genetic analysis using 3 f2 segregating populations derived from crosses between rl10 and the rice lines mian5b, ii-32b, and d62b, respectively, confirmed that the rolled leaf trait of rl10 is controlled by a single recessive gene. of 719 ssr primer pairs that showed polymorphism between d62 ... | 2007 | 17893721 |
| genetic analysis and molecular mapping of a nuclear recessive male sterility gene, ms91(t), in rice. | mutations that result in plant male sterility provide means not only to probe reproductive development but also to facilitate commercial heterosis application and hybrid seed production. in this study, we report a novel male sterility gene, ms91(t), in a spontaneous mutant line (sh38) from a chinese rice cultivar (oryza sativa subsp. japonica 'jijing14'). the sterility of sh38 was studied by examining its progenies derived from crosses with 6 japonica cultivars. corresponding f2 populations were ... | 2007 | 17893719 |
| hereditary behavior of bar gene cassette is complex in rice mediated by particle bombardment. | particle bombardment transformation using minimal gene cassette (containing the promoter, open reading frame and terminator) is the novel trend in plant genetic transformation, and its use helps to alleviate the undesirable effects of plasmid vector backbone sequences on transgenic plants. in the present article, studies related to the hereditary behavior of bar gene cassette in t(1) to t(3) generations of the transgenic rice (oryza sativa l.) lines transformed by particle bombardment have been ... | 2007 | 17884692 |
| wox gene phylogeny in poaceae: a comparative approach addressing leaf and embryo development. | the phylogeny based on the homeodomain (hd) amino acid sequence of the wox (wuschel-related homeobox gene family) was established in the 3 major radiations of the poaceae family: pooideae (brachypodium distachyon), bambusoideae (oryza sativa), and panicoideae (zea mays). the genomes of all 3 grasses contain an ancient duplication in the wox3 branch, and the cellular expression patterns in maize and rice indicate subfunctionalization of paralogues during leaf development, which may relate to the ... | 2007 | 17768306 |
| suppression and promotion of growth by ethylene in rice seedlings depends on ambient humidity. | we examined the effect of ethylene on the growth of rice seedlings (oryza sativa l.) at various degrees of humidity. ethylene significantly suppressed the growth of shoots when applied to seedlings grown under 30% relative humidity (rh), but promoted the growth of shoots when applied to seedlings grown under 100% rh. the application of gibberellic acid (ga(3)) promoted the elongation of shoots in seedlings grown under 30% and 100% rh. ethylene inhibited the shoot elongation induced by ga(3) at 3 ... | 2007 | 17728010 |
| role of oviposition preference in an invasive crambid impacting two graminaceous host crops. | oviposition preference studies of the mexican rice borer, eoreuma loftini (dyar), on sugarcane, saccharum spp., and rice, oryza sativa l., showed that drought stressed sugarcane was 1.8-fold more attractive based on egg masses/plant than well watered sugarcane. the e. loftini susceptible sugarcane cultivar lcp 85-384 was 1.6-fold more attractive than hocp 85-845 based on numbers of eggs per egg mass. egg masses were 9.2-fold more abundant and 2.3-fold larger on sugarcane than on rice. rice, howe ... | 2007 | 17716486 |
| molecular cloning and function analysis of the stay green gene in rice. | chloroplasts undergo drastic morphological and physiological changes during senescence with a visible symptom of chlorophyll (chl) degradation. a stay green mutant was identified and then isolated from the japonica rice (oryza sativa) cv. huazhiwu by gamma-ray irradiation. the stay green mutant was characterized by chl retention, stable chl-protein complexes, and stable thylakoid membrane structures, but lost its photosynthetic competence during senescence. the gene, designated stay green rice ( ... | 2007 | 17714430 |
| rapid reduction of arsenate in the medium mediated by plant roots. | microbes detoxify arsenate by reduction and efflux of arsenite. plants have a high capacity to reduce arsenate, but arsenic efflux has not been reported. tomato (lycopersicon esculentum) and rice (oryza sativa) were grown hydroponically and supplied with 10 microm arsenate or arsenite, with or without phosphate, for 1-3 d. the chemical species of as in nutrient solutions, roots and xylem sap were monitored, roles of microbes and root exudates in as transformation were investigated and efflux of ... | 2007 | 17692074 |
| t-dna tagged knockout mutation of rice osgsk1, an orthologue of arabidopsis bin2, with enhanced tolerance to various abiotic stresses. | t-dna-tagged rice plants were screened under cold- or salt-stress conditions to determine the genes involved in the molecular mechanism for their abiotic-stress response. line 0-165-65 was identified as a salt-responsive line. the gene responsible for this gus-positive phenotype was revealed by inverse pcr as osgsk1 (oryza sativa glycogen synthase kinase3-like gene 1), a member of the plant gsk3/shaggy-like protein kinase genes and an orthologue of the arabidopsis brassinosteroid insensitive 2 ( ... | 2007 | 17690841 |
| a germ cell specific gene of the argonaute family is essential for the progression of premeiotic mitosis and meiosis during sporogenesis in rice. | the rice (oryza sativa) genome contains 18 copies of genes of the argonaute (ago) family. although ago members play important roles in rna-mediated silencing during plant development, a family member that is specifically involved in sexual reproduction has not been identified in plants. we identified the rice ago gene meiosis arrested at leptotene1 (mel1) from the analysis of seed-sterile mutants. in the mel1 mutant, chromosome condensation was arrested at early meiotic stages and irregularly si ... | 2007 | 17675402 |
| involvement of oxidative stress and role of antioxidative defense system in growing rice seedlings exposed to toxic concentrations of aluminum. | when seedlings of rice (oryza sativa l.) cultivar pant-12 were raised in sand cultures containing 80 and 160 mum al(3+) in the medium for 5-20 days, a regular increase in al(3+) uptake with a concomitant decrease in the length of roots as well as shoots was observed. al(3+) treatment of 160 mum resulted in increased generation of superoxide anion (o(2) (-)) and hydrogen peroxide (h(2)o(2)), elevated amount of malondialdehyde, soluble protein and oxidized glutathione and decline in the concentrat ... | 2007 | 17653721 |
| [the correlation between panicle angle and rice quality and genetic analysis on rice quality in japonica rice (oryza sativa l.)]. | linear correlation between panicle angle and 10 quality traits were studied by using p1, p2 and 349 lines of the ril population in a cross of bing 8979(erect panicle)/c bao (curve panicle) in japonica rice. we found that, there were no linear correlation between panicle angle and brown rice rate , head rice rate, chalky grain rate, chalkiness area, gelatinization temperature, gel consistency and apparent amylose content. the correlation coefficients between panicle angle and milled rice rate, be ... | 2007 | 17650489 |
| genetic analysis and mapping of genes involved in fertility of pingxiang dominant genic male sterile rice. | pingxiang dominant genic male sterile rice (pdgmsr) was the first dominant genic male sterile mutant identified in rice (oryza sativa l.), and the corresponding dominant genic male sterile gene was designated as ms-p. the fertility of pdgmsr can be restored by introduction of a dominant epistatic fertility restoring gene in some rice varieties. in the present study, e823, an indica inbred rice variety, restored the fertility of pdgmsr, and the genetic pattern was found to be consistent with a do ... | 2007 | 17643947 |
| influence of epistasis and qtl x environment interaction on heading date of rice (oryza sativa l.). | qtls for heading date of rice (oryza sativa l.) with additive, epistatic, and qtl x environment (qe) interaction effects were studied using a mixed-model-based composite interval mapping (mcim) method and a double haploid (dh) population derived from ir64/azucena in two crop seasons. fourteen qtls conferring heading date in rice, which were distributed on ten chromosomes except for chromosomes 5 and 9, were detected. among these qtls, eight had single-locus effects, five pairs had double-locus i ... | 2007 | 17643946 |
| expression analysis of calcium-dependent protein kinase gene family during reproductive development and abiotic stress conditions in rice (oryza sativa l. ssp. indica). | calcium-dependent protein kinases (cdpks) are important sensors of ca(+2) flux in plants, which control plant development and responses by regulating downstream components of calcium signaling pathways. availability of the whole genome sequence and microarray platform allows investigation of genome-wide organization and expression profile of cdpk genes in rice with a view to ultimately define their function in plant systems. genome-wide analysis led to identification of 31 cdpk genes in rice aft ... | 2007 | 17636330 |
| functional defect at the rice choline monooxygenase locus from an unusual post-transcriptional processing is associated with the sequence elements of short-direct repeats. | glycine betaine (gb), a quaternary ammonium solute, plays a crucial role in developing osmotic tolerance. rice contains a choline monooxygenase (cmo) and two betaine aldehyde dehydrogenase homologues that are required for gb synthesis, but usually no gb is accumulated in rice (oryza sativa). to elucidate the molecular processes that underlie the gb deficiency in rice, an experiment involving rice and spinach (spinacia oleracea) was conducted to analyze the products transcribed from cmo genes. re ... | 2007 | 17635219 |
| metabolism of indole-3-acetic acid in rice: identification and characterization of n-beta-d-glucopyranosyl indole-3-acetic acid and its conjugates. | a search was made for conjugates of indole-3-acetic acid (iaa) in rice (oryza sativa) using liquid chromatography-electrospray ionization-tandem mass spectrometry (lc-esi-ms/ms) in order to elucidate unknown metabolic pathways for iaa. n-beta-d-glucopyranosyl indole-3-acetic acid (iaa-n-glc) was found in an alkaline hydrolysate of rice extract. a quantitative analysis of 3-week-old rice demonstrated that the total amount of iaa-n-glc was equal to that of iaa. a lc-esi-ms/ms-based analysis establ ... | 2007 | 17628621 |
| [effects of simulated nitrogen deposition on competition of weedy species (echinochloa crusgalli var. mitis l.) and upland rice (oryza sativa l.) under different air temperatures]. | a greenhouse experiment was conducted to study the effects of simulated nitrogen deposition on the growth and competition of c4 weedy species (echinochloa crusgalli var. mitis l. ) and c3 upland rice (oryza sativa l.) under day/night temperature of 35 degrees c/25 degrees c and 30 degrees c/20 degrees c. the results showed that under day/night temperature 35 degrees c/25 degrees c, 4.0 g x m(-2) x a(-1) n deposition enhanced the shoot biomass of e. crusgalli and o. sativa by 29.18% and 27.80%, r ... | 2007 | 17615883 |
| development of a cdna microarray to identify gene expression of puccinellia tenuiflora under saline-alkali stress. | puccinellia tenuiflora is the main grass species growing in the saline-alkali soil of the songnen plain in northeastern china, suggesting it has a high tolerance to saline stress. in this study, cdna microarrays containing 1067 clones of p. tenuiflora were constructed to investigate gene expression patterns resulting from saline-alkali (nahco(3)) stress. rna was extracted from p. tenuiflora treated with 400 mmol l(-1) nahco(3) for 6, 12, 24 and 48 h. untreated (no saline-alkali stress) samples w ... | 2007 | 17611115 |
| genome-wide identification of c2h2 zinc-finger gene family in rice and their phylogeny and expression analysis. | transcription factors regulate gene expression in response to various external and internal cues by activating or suppressing downstream genes in a pathway. in this study, we provide a complete overview of the genes encoding c(2)h(2) zinc-finger transcription factors in rice, describing the gene structure, gene expression, genome localization, and phylogenetic relationship of each member. the genome of oryza sativa codes for 189 c(2)h(2) zinc-finger transcription factors, which possess two main ... | 2007 | 17610133 |
| morphological, anatomical and genetic analysis for a rice mutant with abnormal hull. | a mutant with abnormal hull was first discovered from a twin-seedling strain w2555 in rice (oryza sativa l.). the mutant had sparse branches and decreased number of florets from the base to the peak. frequently, the florets at the top of the panicle did not develop completely. the underdeveloped florets often showed slender and white in their life cycle. genetic analysis indicated that the mutant traits were controlled by a single recessive gene (temporarily designated as ah). ah gene controlled ... | 2007 | 17601611 |
| overexpression of the rfca rna recognition motif affects morphologies modifications in rice (oryza sativa l.). | rna recognition motifs as important regulators of gene expression are highly conserved in animals and plants. the fca floral promotion gene in arabidopsis encodes a protein, containing two rna recognition motifs (rrm) and a ww protein interaction domain. here we isolated fca cdna from rice. fca in rice (rfca) was homologous to fca-gamma of arabidopsis and contained conserved domains. to investigate the function of rrm domain, fragment rrm1 and rrm2 of rfca were introduced into rice subspecies or ... | 2007 | 17597396 |
| characterization of ssiiia-deficient mutants of rice: the function of ssiiia and pleiotropic effects by ssiiia deficiency in the rice endosperm. | starch synthase iiia (ssiiia)-deficient rice (oryza sativa) mutants were generated using retrotransposon insertion and chemical mutagenesis. the lowest migrating ss activity bands on glycogen-containing native polyacrylamide gel, which were identified to be those for ssiiia, were completely absent in these mutants, indicating that they are ssiiia null mutants. the amylopectin b(2) to b(4) chains with degree of polymerization (dp) >/= 30 and the m(r) of amylopectin in the mutant were reduced to a ... | 2007 | 17586688 |
| the identification of candidate rice genes that confer resistance to the brown planthopper (nilaparvata lugens) through representational difference analysis. | the development of rice varieties (oryza sativa l.) that are resistant to the brown planthopper (bph; nilaparvata lugens stål) is an important objective in current breeding programs. in this study, we generated 132 bc(5)f(5) near-isogenic rice lines (nils) by five backcrosses of samgangbyeo, a bph resistant indica variety carrying the bph1 locus, with nagdongbyeo, a bph susceptible japonica variety. to identify genes that confer bph resistance, we employed representational difference analysis (r ... | 2007 | 17585380 |
| du1, encoding a novel prp1 protein, regulates starch biosynthesis through affecting the splicing of wxb pre-mrnas in rice (oryza sativa l.). | starch is the major component of cereal grains. in rice, starch properties determine the eating and cooking quality. the dull endosperm of rice grains is a classical morphological and agronomical trait that has long been exploited for breeding and genetics study. to understand the molecular mechanism that regulates the starch biosynthesis in rice grains, we characterized a classic rice mutant dull endosperm1 (du1) and isolated du1 through a map-based cloning approach. du1, encoding a member of p ... | 2007 | 17579813 |
| high-throughput multiplex microsatellite marker assay for detection and quantification of adulteration in basmati rice (oryza sativa). | basmati rice is a very special type of aromatic rice known world-wide for its extra long grains and pleasant and distinct aroma. traditional basmati rice cultivars, confined to indo-gangetic regions of the indian subcontinent, are often reported to be adulterated with crossbred basmati varieties and long-grain non-basmati varieties in the export market. at present, there is no commercial scale technology to reliably detect adulteration. we report here a ce-based multiplex microsatellite marker a ... | 2007 | 17577195 |
| predicting the size of the progeny mapping population required to positionally clone a gene. | a key frustration during positional gene cloning (map-based cloning) is that the size of the progeny mapping population is difficult to predict, because the meiotic recombination frequency varies along chromosomes. we describe a detailed methodology to improve this prediction using rice (oryza sativa l.) as a model system. we derived and/or validated, then fine-tuned, equations that estimate the mapping population size by comparing these theoretical estimates to 41 successful positional cloning ... | 2007 | 17565938 |
| qtl mapping and qxe interactions of grain cooking and nutrient qualities in rice under upland and lowland environments. | grain cooking and nutrient qualities are the most important components of rice (oryza sativa l.) quality. a doubled haploid (dh) population from a cross between two japonica cultivars was used to examine the phenotypic values and potential qtls for the quality traits. the cooking and nutrient quality traits, including the amylose content (ac), the gel consistency (gc), the gelatinization temperature (gt), and the protein content (pc), in rice grown under upland and lowland environments were eval ... | 2007 | 17560528 |
| a quantitative rt-pcr platform for high-throughput expression profiling of 2500 rice transcription factors. | quantitative reverse transcription - polymerase chain reaction (qrt-pcr) has been demonstrated to be particularly suitable for the analysis of weakly expressed genes, such as those encoding transcription factors. rice (oryza sativa l.) is an important crop and the most advanced model for monocotyledonous species; its nuclear genome has been sequenced and molecular tools are being developed for functional analyses. however, high-throughput methods for rice research are still limited and a large-s ... | 2007 | 17559651 |
| highly asymmetric rice genomes. | individuals in the same species are assumed to share the same genomic set. however, it is not unusual to find an orthologous gene only in small subset of the species, and recent genomic studies suggest that structural rearrangements are very frequent between genomes in the same species. two recently sequenced rice genomes oryza sativa l. var. nipponbare and o. sativa l. var. 93-11 provide an opportunity to systematically investigate the extent of the gene repertoire polymorphism, even though the ... | 2007 | 17555605 |
| overexpression of oscoin, a putative cold inducible zinc finger protein, increased tolerance to chilling, salt and drought, and enhanced proline level in rice. | rice (oryza sativa l.) plant is sensitive to chilling, particularly at early stages of seedling development. here a novel cold-inducible gene, designated oscoin (oryza sativa cold-inducible), was isolated and characterized. results showed that oscoin protein, a ring finger protein, was localized in both nuclear and cytoplasm membrane. oscoin is expressed in all rice organs and strongly induced by low temperature, aba, salt and drought. over-expression of oscoin in transgenic rice lines significa ... | 2007 | 17549515 |
| fine mapping of s31, a gene responsible for hybrid embryo-sac abortion in rice (oryza sativa l.). | partial abortion of female gametes and the resulting semi-sterility of indica x japonica inter-subspecific rice hybrids have been ascribed to an allelic interaction, which can be avoided by the use of wide compatibility varieties. to further understand the genetic mechanism of hybrid sterility, we have constructed two sets of hybrids, using as male parent either the typical japonica variety asominori, or the wide compatibility variety 02428; and as female, a set of 66 chromosome segment substitu ... | 2007 | 17549514 |
| [cloning and characterization of rzf71 encoding a c2h2-type zinc finger protein from rice]. | a rice zinc-finger protein gene, rzf71, encoding the c2h2-type zinc-finger transcription factor was isolated from rice (oryza sativa l. subs. japonica) by rt-pcr approach. gene rzf71 encodes a 25 kda protein with 250 amino acids, which contains two typical c2h2 zinc finger domains. the expression profiling showed that rzf71 was constitutively expressed in roots, culms, leaves, and flowering spikes. the semi-quantitative rt-pcr assay showed rzf71 was strongly induced by high-salinity and 20% peg6 ... | 2007 | 17548332 |
| genetic variation in the chloroplast genome suggests multiple domestication of cultivated asian rice (oryza sativa l.). | two hundred and seventy-five accessions of cultivated asian rice and 44 accessions of aa genome oryza species were classified into 8 chloroplast (cp) genome types (a-h) based on insertion-deletion events at 3 regions (8k, 57k, and 76k) of the cp genome. the ancestral cp genome type was determined according to the frequency of occurrence in oryza species and the likely evolution of the variable 57k region of the cp genome. when 2 nucleotide substitutions (aa or tt) were taken into account, these ... | 2007 | 17546083 |
| identification and fine mapping of a thermo-sensitive chlorophyll deficient mutant in rice (oryza sativa l.). | a thermo-sensitive chlorophyll deficient mutant was isolated from more than 15,000 transgenic rice lines. the mutant displayed normal phenotype at 23 degrees c or lower temperature (permissive temperature). however, when grown at 26 degrees c or higher (nonpermissive temperature) the plant exhibited an abnormal phenotype characterized by yellow green leaves. genetic analysis revealed that a single nuclear-encoded recessive gene is responsible for the mutation, which is tentatively designed as cd ... | 2007 | 17541632 |
| effects of nitrogen and potassium fertilizers on rice (oryza sativa l.) genotypes processing characteristics. | in order to consider effects of various levels of nitrogen and potassium application on kernel processing characteristics of rice cultivars, an experiment was developed in 2004 and 2005 using tarrom (a local iranian landrace) and neda (an iranian improved cultivar). in this experiment four levels of nitrogen fertilizer (0, 50, 100 and 150 kg n ha(-1) from urea source) and four levels of potassium fertilizer (0, 75, 150 and 225 kg k2o ha(-1) from potassium sulfate source) have been applied using ... | 2007 | 19086545 |
| characterization of stress-responsive cipk genes in rice for stress tolerance improvement. | plants respond to adverse environments by initiating a series of signaling processes that often involves diverse protein kinases, including calcineurin b-like protein-interacting protein kinases (cipks). in this study, putative cipk genes (oscipk01-oscipk30) in the rice (oryza sativa) genome were surveyed for their transcriptional responses to various abiotic stresses. the results showed that 20 oscipk genes were differentially induced by at least one of the stresses, including drought, salinity ... | 2007 | 17535819 |
| a collection of 10,096 indica rice full-length cdnas reveals highly expressed sequence divergence between oryza sativa indica and japonica subspecies. | relatively few indica rice full-length cdnas were available to aid in the annotation of rice genes. the data presented here described the sequencing and analysis of 10,096 full-length cdnas from oryza sativa subspecies indica guangluai 4. of them, 9,029 matched rice genomic sequences in publicly-available databases, and 1,200 were identified as new rice genes. comparison with the knowledge-based oryza molecular biological encyclopedia japonica cdna collection indicated that 3,316 (41.6%) of the ... | 2007 | 17522955 |
| biochemical properties of a plastidial dna polymerase of rice. | plastids are organelles unique to plant cells and are responsible for photosynthesis and other metabolic functions. despite their important cellular roles, relatively little is known about the mechanism of plastidial dna replication and repair. recently, we identified a novel dna polymerase in oryza sativa l. (ospolp1, formerly termed ospoli-like) that is homologous to prokaryotic dna polymerase is (polis), and suggested that this polymerase might be involved in plastidial dna replication and re ... | 2007 | 17522954 |
| a cytosolic nadp-malic enzyme gene from rice (oryza sativa l.) confers salt tolerance in transgenic arabidopsis. | nadp-malic enzyme (nadp-me, ec 1.1.1.40) functions in many different pathways in plant and may be involved in plant defense such as wound and uv-b radiation. here, expression of the gene encoding cytosolic nadp-me (cytonadp-me, genbank accession no. ay444338) in rice (oryza sativa l.) seedlings was induced by salt stress (nacl). nadp-me activities in leaves and roots of rice also increased in response to nacl. transgenic arabidopsis plants over-expressing rice cytonadp-me had a greater salt tole ... | 2007 | 17516134 |
| physiological character and gene mapping in a new green- revertible albino mutant in rice. | a green-revertible albino mutant-qiufeng m was found from the japonica rice (oryza sativa l. ssp. japonica) qiufeng in the field. the first three leaves of the mutant were albino with some green. the leaf color became pale green since the fourth leaf and the glume had the same phenomenon as the first three leaves. the measuring data of the pigment content confirmed the visually observed results. it truly had a remarkable changing process in the leaf color in qiufeng m. comparison of the main agr ... | 2007 | 17498631 |
| genome-wide detection and analysis of alternative splicing for nucleotide binding site-leucine-rich repeats sequences in rice. | alternative splicing is a major contributor to genomic complexity and proteome diversity, yet the analysis of alternative splicing for the sequence containing nucleotide binding site and leucine-rich repeats (nbs-lrr) domain has not been explored in rice (oryza sativa l.). hidden markov model (hmm) searches were performed for nbs-lrr domain. 875 nbs-lrr-encoding sequences were obtained from the institute for genomic research (tigr). all of them were used to blast knowledge-based oryza molecular ... | 2007 | 17498622 |
| irap and remap assessments of genetic similarity in rice. | rice is a model genome for cereal research, providing important information about genome structure and evolution. retrotransposons are common components of grass genomes, showing activity at transcription, translation and integration levels. their abundance and ability to transpose make them good potential markers. in this study, we used 2 multilocus pcr-based techniques that detect retrotransposon integration events in the genome: irap (inter-retrotransposon amplified polymorphism) and remap (r ... | 2007 | 17495343 |
| study of growth factors, alpha-amylase and peroxidase activity in various cultivars of rice (oryza sativa l.) under vanillic acid stress. | the effects of vanillic acid (va) on germination, seedling and adult plant of rice (oryza sativa l.) were investigated. four cultivars, traditional (taroom mahalli and taroom deilamani) and improved (shafagh and onda) were studied. for germination, seeds were sterilized and then placed on petri dish at 30 degrees c at different concentrations (0, 10, 20 and 25 mm) for 7 days and growth factors of seedling were measured after 14 days. seedling (10 days) planted in hydroponic medium including nutr ... | 2007 | 19086516 |
| a novel wx mutation caused by insertion of a retrotransposon-like sequence in a glutinous cultivar of rice (oryza sativa). | dna polymorphism of the wx gene in glutinous rice cultivars was investigated by pcr-rf-sscp and heteroduplex cleavage analysis using brassica petiole extract, and the nucleotide sequence variations were identified. most japonica-type glutinous rice was found to have a 23-bp duplication in the second exon, which causes loss of the function of granule-bound starch synthase (gbss) encoded by the wx gene. without the 23-bp duplication, there was an insertion of 7,764 bp in the ninth exon of the wx a ... | 2007 | 17492423 |
| identification and linkage mapping of complementary recessive genes causing hybrid breakdown in an intraspecific rice cross. | one outcome of hybrid breakdown is poor growth, which we observed as a reduction in the number of panicles per plant and in culm length in an f(2) population derived from a cross between the genetically divergent rice (oryza sativa l.) cultivars 'sasanishiki' (japonica) and 'habataki' (indica). quantitative trait locus (qtl) analysis of the two traits and two-way anova of the detected qtls suggested that the poor growth was due mainly to an epistatic interaction between genes at qtls located on ... | 2007 | 17486310 |
| rnai knockdown of oryza sativa root meander curling gene led to altered root development and coiling which were mediated by jasmonic acid signalling in rice. | jasmonic acid (ja) is a well-known defence hormone, but its biological function and mechanism in rice root development are less understood. here, we describe a ja-induced putative receptor-like protein (osrlk, aal87185) functioning in root development in rice. rna in situ hybridization revealed that the gene was expressed largely in roots, and a fusion protein showed its localization on the plasma membrane. the primary roots in rnai transgenic rice plants meandered and curled more easily than wi ... | 2007 | 17470145 |
| analysis of the conditional correlations from different genetic systems between the protein content and the appearance quality traits of indica rice. | a factorial mating design in two environments was conducted using 7 cytoplasmic male sterile lines (a) and 5 restorer lines (r) along with their f(1) (a x r) and f(2) populations. the unconditional and conditional analyses of genetic models and the corresponding statistic methods, including endospermic, cytoplasmic, and maternal plant genetic systems, were used to analyze the genetic relationships between protein content (pc) and the appearance quality traits of indica rice (oryza sativa l.). th ... | 2007 | 17469785 |
| lazy1 controls rice shoot gravitropism through regulating polar auxin transport. | tiller angle of rice (oryza sativa l.) is an important agronomic trait that contributes to grain production, and has long attracted attentions of breeders for achieving ideal plant architecture to improve grain yield. although enormous efforts have been made over the past decades to study mutants with extremely spreading or compact tillers, the molecular mechanism underlying the control of tiller angle of cereal crops remains unknown. here we report the cloning of the lazy1 (la1) gene that regul ... | 2007 | 17468779 |
| expression profiling of oryza sativa metal homeostasis genes in different rice cultivars using a cdna macroarray. | rice is an important food crop, but it is a poor source of essential micronutrients such as iron and zinc. in order to improve the metal ion content of rice grains through breeding or biotechnology, more information is needed on the molecular players that help mobilize metals from leaves to developing seeds. to profile several genes simultaneously, a cdna macroarray was developed using 36 metal-related genes from rice, including zips, nramps, and ysls (coding for known or potential metal transpo ... | 2007 | 17468002 |
| response of rice cultivars to rates of nitrogen and potassium application in field and pot conditions. | nitrogen and potassium are the yield-limiting nutrients in rice production regions of iran. use of n and k efficient cultivars is an important complementary strategy in improving rice yield, increasing the quality properties of rice grains and reducing cost of production. in order to consider the effects of different amounts of n and k application on rice (oryza sativa l.) yield and yield components in pot and field conditions these experiments were undertaken in 2004 at sari agricultural statio ... | 2007 | 19069953 |
| comprehensive analysis of the expression of twenty-seven beta-1, 3-glucanase genes in rice (oryza sativa l.). | plant beta-1, 3-glucanases are involved in plant defense and in development. very little data are available on the expression of rice glucanases both in developmental tissues and under various stresses. in this study, we cloned and characterized twenty-seven rice beta-1, 3-glucanases (osglu) from at total of 71 putative glucanases. the osglu genes were obtained by pcr from a cdna library and were classified into seven groups (group i to vii) according to their dna or amino acid sequence homology ... | 2007 | 17464198 |
| overexpression of sbpase enhances photosynthesis against high temperature stress in transgenic rice plants. | activity of the calvin cycle enzyme sedoheptulose-1,7-bisphosphatase (sbpase) was increased by overexpression of a rice plants 9,311 (oryza sativa l.) cdna in rice plants zhonghua11 (oryza sativa l.). the genetic engineering enabled the plants to accumulate sbpase in chloroplasts and resulted in enhanced tolerance to high temperature stress during growth of young seedlings. moreover, co(2) assimilation of transgenic plants was significantly more tolerant to high temperature than that of wild-typ ... | 2007 | 17458549 |
| rubidium chloride tolerant callus cultures of rice (oryza sativa l.) accumulate more potassium and cross tolerate to other salts. | callus cultures from salt tolerant (csr-10) and susceptible (swarnadhan) varieties of oryza sativa l. were established in murashige and skoog's (ms) medium containing lethal concentrations (50 mm) of rubidium chloride (rbcl) as a selective agent. while 95-100% cells were viable in callus cultures grown without rbcl, viability was 75% in 50 mm rbcl selected cultures. growth of rbcl selected calli in presence of salt was comparable to that of callus grown without it. cells tolerant to rbcl showed ... | 2007 | 17453215 |
| high temperature stress and spikelet fertility in rice (oryza sativa l.). | in future climates, greater heat tolerance at anthesis will be required in rice. the effect of high temperature at anthesis on spikelet fertility was studied on ir64 (lowland indica) and azucena (upland japonica) at 29.6 degrees c (control), 33.7 degrees c, and 36.2 degrees c tissue temperatures. the objectives of the study were to: (i) determine the effect of temperature on flowering pattern; (ii) examine the effect of time of day of spikelet anthesis relative to a high temperature episode on s ... | 2007 | 17431025 |
| responses of rice cultivars with different nitrogen use efficiency to partial nitrate nutrition. | there is increased evidence that partial nitrate (no3-) nutrition (pnn) improves growth of rice (oryza sativa), although the crop prefers ammonium (nh4+) to no3- nutrition. it is not known whether the response to no3- supply is related to nitrogen (n) use efficiency (nue) in rice cultivars. methods solution culture experiments were carried out to study the response of two rice cultivars, nanguang (high-nue) and elio (low-nue), to partial no3- supply in terms of dry weight, n accumulation, grain ... | 2007 | 17428833 |
| genome-wide identification and analyses of the rice calmodulin and related potential calcium sensor proteins. | a wide range of stimuli evoke rapid and transient increases in [ca2+]cyt in plant cells which are transmitted by protein sensors that contain ef-hand motifs. here, a group of oryza sativa l. genes encoding calmodulin (cam) and cam-like (cml) proteins that do not possess functional domains other than the ca2+-binding ef-hand motifs was analyzed. | 2007 | 17263873 |
| distinct signalling pathways for induction of map kinase activities by cadmium and copper in rice roots. | plant growth is severely affected by toxic concentrations of heavy metals. on characterizing the heavy metal-induced signalling pathways, the effects of cadmium (cdcl2) and copper (cucl2) on mbp (myelin basic protein) kinase activities in oryza sativa l. cv. tng67 were analysed and it was found that cd2+-induced 42 kda mbp kinase has the characteristics of a mitogen-activated protein (map) kinase. this study confirmed that the 42 kda kinase-active band contains, at least, the activities of osmpk ... | 2007 | 17259646 |
| spatial distribution and temporal variation of the rice silicon transporter lsi1. | rice (oryza sativa) is a typical silicon (si) accumulator and requires a large amount of si for high-yield production. recently, a gene (low silicon rice1 [lsi1]) encoding a si transporter was identified in rice roots. here, we characterized lsi1 in terms of spatial distribution and temporal variation using both physiological and molecular approaches. results from a multicompartment transport box experiment showed that the major site for si uptake was located at the basal zone (>10 mm from the r ... | 2007 | 17259286 |
| cultivar identification of rice (oryza sativa l.) by polymerase chain reaction method and its application to processed rice products. | as the cultivars of rice markedly affect eating quality, processing suitability, and price, identification or differentiation of rice cultivar is very important. we developed suitable 14 sts (sequence-tagged site) primers for pcr (polymerase chain reaction), and it became possible to differentiate 60 japanese dominant rice cultivars from each other using template dna extracted and purified from rice grains. a multiplex primer set was shown to be useful to effectively differentiate rice cultivars ... | 2007 | 17256960 |
| rapid discrimination of scented rice by solid-phase microextraction, mass spectrometry, and multivariate analysis used as a mass sensor. | this study describes a new and suitable method for the rapid evaluation of rice (oryza sativa, l.) aroma by analysis of the volatile fraction using solid-phase microextraction coupled with mass spectrometry (spme/ms). the abundance list of unresolved mass fragments of the spme extracted volatile fraction formed the "fingerprint" of a rice sample. fingerprints of 61 rice samples were recorded in duplicate. pollutants originating from the extraction system induce fingerprint background that could ... | 2007 | 17256955 |
| identification of drought-induced micrornas in rice. | micrornas (mirnas) are a large new class of small non-coding rnas. to date, hundreds of micrornas have been identified in plants. micrornas play important roles in post-transcriptional gene regulation by targeting mrnas for cleavage or repressing translation. to better understand microrna function, we have used an oligonucleotide microarray to monitor rice (oryza sativa) microrna expression profile under drought stress. two drought-induced micrornas were identified. furthermore, mir-169g was con ... | 2007 | 17254555 |
| chemically induced expression of rice osb2 under the control of the ospr1.1 promoter confers increased anthocyanin accumulation in transgenic rice. | anthocyanin pigmentation provides an excellent system with which to study the regulation of gene expression in higher plants. in this study, ospr1.1 promoter was isolated and the promoter activity was monitored using a reporter gene osb2, which encodes a transcription factor for anthocyanin synthesis in rice plants. we introduced pr::osb2 plasmid into an isogenic taichung 65, no. 99-962 t-65 cba b9f5 (t65 cba), rice line (oryza sativa l.) and found that the transgenic rice plants exhibited antho ... | 2007 | 17253710 |
| expression of an nadp-malic enzyme gene in rice (oryza sativa. l) is induced by environmental stresses; over-expression of the gene in arabidopsis confers salt and osmotic stress tolerance. | nadp-malic enzyme (nadp-me, ec 1.1.1.40) functions in many different pathways in plants, and has recently been implicated in plant defense such as in responses to wounding and uv-b radiation. in this study, we isolated a complementary dna (cdna) clone by using the differential display method and screening of a root cdna library of rice (oryza sativa. l) under carbonate (nahco3) stress, and identified it as one of the rice nadp-me genes (we named it nadp-me2, genbank accession no. ab053295). the ... | 2007 | 17245561 |
| transport mechanisms for the uptake of organic compounds by rice (oryza sativa) roots. | uptake of six organic compounds, dinitrobenzene (dnb), dinitrotoluene (dnt), lindan (lin), 1,2,3-triclorobenzene (tcb), phenanthrene (phn) and pyrene (pyr) by freshly excised rice roots and dead rice roots (heated for 40min at 105 degrees c) were investigated. results indicated that the uptake by the two types of roots did not increase proportionally with those in external solution. there appears to be some special chemical function of root cells other than simple absorption by the cells. the co ... | 2007 | 17240019 |
| biased distribution of microsatellite motifs in the rice genome. | microsatellites are useful tools to study the extent of divergence between two taxonomic groups that show high sequence similarity. we have compared microsatellite distribution to illustrate genetic variation between the two rice genomes, oryza sativa l. ssp. indica and oryza sativa l. ssp. japonica. microsatellite distribution proved to be non random as certain regions of very high microsatellite density have been identified. microsatellite density in the subspecies japonica was computed margin ... | 2007 | 17237941 |
| knockout of a starch synthase gene osssiiia/flo5 causes white-core floury endosperm in rice (oryza sativa l.). | to elucidate the role of ssiiia during starch synthesis in rice (oryza sativa l.) endosperm, we characterized null mutants of this gene, generated by t-dna insertions. scanning electron microscope (sem) analysis revealed that the starch granules in these mutants are smaller and rounder compared with the wild type controls, and that the mutant endosperm is characterized by a loosely packed central portion exhibiting a floury-like phenotype. hence, the osssiiia (oryza sativa ssiiia) mutations are ... | 2007 | 17297616 |
| computational identification and phylogenetic analysis of the mapk gene family in oryza sativa. | the mapk cascade plays greatly important roles in signal transduction pathways. the present study computationally identified 16 rice mapks (osmpks). the results of est and cdna hitting supported the reliability of osmpks in rice. gene structure comparison showed large differences in exon numbers, from 2 to 12, among members of the rice mapk family. rice mapks were located on chromosomes 1, 2, 3, 5, 6, 10, and 11, particularly being distributed on the 1, 5 and 6 chromosomes. on a genome scale we ... | 2007 | 17296305 |
| oryzacystatin-ii, a cystatin from rice (oryza sativa l. japonica), is a dimeric protein: possible involvement of the interconversion between dimer and monomer in the regulation of the reactivity of oryzacystatin-ii. | we examined the biochemical and structural properties of oryzacystatin-ii, a phytocystatin in rice (oryza sativa l. japonica), under heat-stress conditions. the enzyme inhibitory reactivity of oryzacystatin-ii was enhanced by heating in a temperature-dependent manner and reached a maximum level by heating at 65 degrees c for 10 min. size-exclusion chromatography showed that oryzacystatin-ii forms a homodimer at ambient temperature and that the enhancement of inhibitory reactivity is due to the c ... | 2007 | 17295505 |
| rice as a model for centromere and heterochromatin research. | rice (oryza sativa) has become an important model plant species in numerous research projects involving genome, molecular and evolutionary biology. in this review we describe the reasons why rice provides an excellent model system for centromere and heterochromatin research. in most multicellular eukaryotes, centromeres and heterochromatic domains contain long arrays of repetitive dna elements that are recalcitrant to dna sequencing. in contrast, three rice centromeres and the majority of the cy ... | 2007 | 17295128 |
| f-box proteins in rice. genome-wide analysis, classification, temporal and spatial gene expression during panicle and seed development, and regulation by light and abiotic stress. | f-box proteins constitute a large family in eukaryotes and are characterized by a conserved f-box motif (approximately 40 amino acids). as components of the skp1p-cullin-f-box complex, f-box proteins are critical for the controlled degradation of cellular proteins. we have identified 687 potential f-box proteins in rice (oryza sativa), the model monocotyledonous plant, by a reiterative database search. computational analysis revealed the presence of several other functional domains, including le ... | 2007 | 17293439 |
| overexpression of a type-a response regulator alters rice morphology and cytokinin metabolism. | genome-wide analyses of rice (oryza sativa l.) cytokinin (ck)-responsive genes using the affymetrix genechip(r) rice genome array were conducted to define the spectrum of genes subject to regulation by ck in monocotyledonous plants. application of trans-zeatin modulated the expression of a wide variety of genes including those involved in hormone signaling and metabolism, transcriptional regulation, macronutrient transport and protein synthesis. to understand further the function of ck in rice p ... | 2007 | 17293362 |
| oryza sativa myosin xi b controls pollen development by photoperiod-sensitive protein localizations. | myosins are actin-based motor proteins responsible for various motility and signal transduction. only a small set of myosin classes is present inplants, and little is known about their functions. here we showed how a rice myosin gene controlled pollen development by sensing changed environmental factors. the analysis is based on a gene-trapped ds insertion mutant oryza sativa myosin xi b (osmyoxib). this mutant showed male sterility under short day length (sd) conditions and fertility under long ... | 2007 | 17289016 |
| two novel loci for pollen sterility in hybrids between the weedy strain ludao and the japonica variety akihikari of rice (oryza sativa l.). | partial pollen sterility has been observed in hybrid progeny derived from a japonica cultivar, akihikari and a weedy strain, ludao, which naturally grows in jiangsu province of east china. cytological and histological analyses revealed that pollen abortion occurred largely at the bicellular pollen stage, primarily due to the gradual disaggregation of generative and vegetative cells. a genome-wide analysis was further carried out in a backcross population of akihikari //ludao/akihikari using a to ... | 2007 | 17287976 |
| fine mapping of pss1, a pollen semi-sterile gene in rice (oryza sativa l.). | during routine seed increase procedures in rice, semi-sterile plants are common; however, such semi-sterility mutants in rice varieties have been only rarely analyzed genetically. w207-2 is a semi-sterile selection from the japonica rice variety nipponbare. in this report, we found the female gamete of w207-2 was normal, and its semi-sterility was unaffected by growth duration but was conditioned by a recessive nuclear gene whose action leads to pollen semi-sterility and anther indehiscence, and ... | 2007 | 17279367 |
| establishment of an in vitro fertilization system in rice (oryza sativa l.). | in vitro fertilization (ivf) systems using isolated male and female gametes have been utilized to dissect fertilization-induced events in angiosperms, such as egg activation, zygote development and early embryogenesis, as the female gametophytes of plants are deeply embedded within ovaries. in this study, a rice ivf system was established to take advantage of the abundant resources stemming from rice research for investigations into the mechanisms of fertilization and early embryogenesis. fusion ... | 2007 | 17361458 |
| bayesian association mapping of multiple quantitative trait loci and its application to the analysis of genetic variation among oryza sativa l. germplasms. | one way to use a crop germplasm collection directly to map qtls without using line-crossing experiments is the whole genome association mapping. a major problem with association mapping is the presence of population structure, which can lead to both false positives and failure to detect genuine associations (i.e., false negatives). particularly in highly selfing species such as asian cultivated rice, high levels of population structure are expected and therefore the efficiency of association map ... | 2007 | 17356864 |
| expression of ascorbate peroxidase 8 in roots of rice (oryza sativa l.) seedlings in response to nacl. | reactive oxygen species are thought to play an important role in nacl stress. therefore, the expression patterns of the gene family encoding the h(2)o(2)-scavenging enzyme ascorbate peroxidase (apx; ec1.11.1.11) were analysed in roots of etiolated rice (oryza sativa l.) seedlings in response to nacl stress. applying semi-quantitative rt-pcr, the mrna levels were quantified for two cytosolic (osapx1 and osapx2), two peroxisomal (osapx3 and osapx4), and four chloroplastic (osapx5, osapx6, osapx7, ... | 2007 | 17916638 |
| differential gene expression in an elite hybrid rice cultivar (oryza sativa, l) and its parental lines based on sage data. | it was proposed that differentially-expressed genes, aside from genetic variations affecting protein processing and functioning, between hybrid and its parents provide essential candidates for studying heterosis or hybrid vigor. based our serial analysis of gene expression (sage) data from an elite chinese super-hybrid rice (lyp9) and its parental cultivars (93-11 and pa64s) in three major tissue types (leaves, roots and panicles) at different developmental stages, we analyzed the transcriptome ... | 2007 | 17877838 |
| an expression atlas of rice mrnas and small rnas. | identification of all expressed transcripts in a sequenced genome is essential both for genome analysis and for realization of the goals of systems biology. we used the transcriptional profiling technology called 'massively parallel signature sequencing' to develop a comprehensive expression atlas of rice (oryza sativa cv nipponbare). we sequenced 46,971,553 mrna transcripts from 22 libraries, and 2,953,855 small rnas from 3 libraries. the data demonstrate widespread transcription throughout the ... | 2007 | 17351617 |
| assimilation of ammonium ions and reutilization of nitrogen in rice (oryza sativa l.). | a major source of inorganic nitrogen for rice plants grown in paddy soil is ammonium ions. the ammonium ions are actively taken up by the roots via ammonium transporters and subsequently assimilated into the amide residue of glutamine (gln) by the reaction of glutamine synthetase (gs) in the roots. the gln is converted into glutamate (glu), which is a central amino acid for the synthesis of a number of amino acids, by the reaction of glutamate synthase (gogat). although a small gene family for b ... | 2007 | 17350935 |
| osmtn encodes a 5'-methylthioadenosine nucleosidase that is up-regulated during submergence-induced ethylene synthesis in rice (oryza sativa l.). | methylthioadenosine (mta) is released as a by-product of s-adenosylmethionine (adomet)-dependent reactions central to ethylene, polyamine, or phytosiderophore biosynthesis. mta is hydrolysed by methylthioadenosine nucleosidase (mtn; ec 3.2.2.16) into adenine and methylthioribose which is processed through the methionine (met) cycle to produce a new molecule of adomet. in deepwater rice, submergence enhances ethylene biosynthesis, and ethylene in turn influences the methionine cycle through posit ... | 2007 | 17339651 |
| comparative physical mapping between oryza sativa (aa genome type) and o. punctata (bb genome type). | a comparative physical map of the aa genome (oryza sativa) and the bb genome (o. punctata) was constructed by aligning a physical map of o. punctata, deduced from 63,942 bac end sequences (bess) and 34,224 fingerprints, onto the o. sativa genome sequence. the level of conservation of each chromosome between the two species was determined by calculating a ratio of bes alignments. the alignment result suggests more divergence of intergenic and repeat regions in comparison to gene-rich regions. fur ... | 2007 | 17339227 |
| the flowering locus t-like gene family in barley (hordeum vulgare). | the flowering locus t (ft) gene plays a central role in integrating flowering signals in arabidopsis because its expression is regulated antagonistically by the photoperiod and vernalization pathways. ft belongs to a family of six genes characterized by a phosphatidylethanolamine-binding protein (pebp) domain. in rice (oryza sativa), 19 pebp genes were previously described, 13 of which are ft-like genes. five ft-like genes were found in barley (hordeum vulgare). hvft1, hvft2, hvft3, and hvft4 we ... | 2007 | 17339225 |
| rice (oryza sativa) allergy in rhinitis and asthma patients: a clinico-immunological study. | sensitization to foods varies in different countries reflecting a possible interaction of genetic factors, cultural and dietary habits. rice is a major food consumed world wide and needs evaluation for ige mediated reactions. the present study was carried out to identify rice allergy in patients of rhinitis and asthma and identify the allergenic proteins in raw and cooked rice. of 1200 patients screened using standard questionnaire, 165 presented with history of rice allergy. of these, 20 (12.1% ... | 2007 | 17336834 |
| nitrite-driven anaerobic atp synthesis in barley and rice root mitochondria. | mitochondria isolated from the roots of barley (hordeum vulgare l.) and rice (oryza sativa l.) seedlings were capable of oxidizing external nadh and nadph anaerobically in the presence of nitrite. the reaction was linked to atp synthesis and nitric oxide (no) was a measurable product. the rates of nadh and nadph oxidation were in the range of 12-16 nmol min(-1) mg(-1) protein for both species. the anaerobic atp synthesis rate was 7-9 nmol min(-1) mg(-1) protein for barley and 15-17 nmol min(-1) ... | 2007 | 17333252 |
| reduced protease activity in transformed rice cell suspension cultures expressing a proteinase inhibitor. | in this study, we synthesized a synthetic serine proteinase inhibitor ii gene (spi-ii) that harbored the chymotrypsin and trypsin inhibitor domains of the pi-ii gene from nicotiana alata. in an effort to reduce protease activity in a rice cell suspension culture, we first synthesized spi-ii using overlap pcr and then introduced the gene into a rice calli (oryza sativa l. cv. dongin) by particle bombardment-mediated transformation. the spi-ii gene was under the control of a rice alpha-amylase 3d ... | 2007 | 17314052 |
| a model explaining genotypic and ontogenetic variation of leaf photosynthetic rate in rice (oryza sativa) based on leaf nitrogen content and stomatal conductance. | identification of physiological traits associated with leaf photosynthetic rate (pn) is important for improving potential productivity of rice (oryza sativa). the objectives of this study were to develop a model which can explain genotypic variation and ontogenetic change of pn in rice under optimal conditions as a function of leaf nitrogen content per unit area (n) and stomatal conductance (g(s)), and to quantify the effects of interaction between n and g(s) on the variation of pn. | 2007 | 17204541 |
| dramatic reduction of crop-to-crop gene flow within a short distance from transgenic rice fields. | genetically modified (gm) rice with enhanced agronomic traits and pharmaceutical uses are ready for widespread adoption. little is known about isolation requirements for achieving stringent transgene confinement in rice. to investigate the extent of pollen-mediated crop-to-crop transgene flow, we conducted a field experiment with four plot-size treatments of adjacent gm and nongm rice (oryza sativa) in china. three insect-resistant gm rice (bt/cpti) and nongm isogenic lines were used in the stud ... | 2007 | 17204081 |
| the discriminatory transfer routes of trna genes among organellar and nuclear genomes in flowering plants: a genome-wide investigation of indica rice. | the transfer and integration of trna genes from organellar genomes to the nuclear genome and between organellar genomes occur extensively in flowering plants. the routes of the genetic materials flowing from one genome to another are biased, limited largely by compatibility of dna replication and repair systems differing among the organelles and nucleus. after thoroughly surveying the trna gene transfer among organellar genomes and the nuclear genome of a domesticated rice (oryza sativa l. ssp. ... | 2007 | 17273918 |