Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| mechanisms of biodegradation of dibenzoate plasticizers. | biodegradation mechanisms were elucidated for three dibenzoate plasticizers: diethylene glycol dibenzoate (d(eg)db), dipropylene glycol dibenzoate (d(pg)db), both of which are commercially available, and 1,6-hexanediol dibenzoate, a potential green plasticizer. degradation studies were done using rhodococcus rhodochrous in the presence of pure alkanes as a co-substrate. as expected, the first degradation step for all of these systems was the hydrolysis of one ester bond with the release of benzo ... | 2009 | 19665165 |
| evaluation of plasma fibrinogen concentration as an indicator of physeal or epiphyseal osteomyelitis in foals: 17 cases (2002-2007). | to determine whether high plasma fibrinogen concentration (> or = 900 mg/dl) is a valid indicator of physeal or epiphyseal osteomyelitis in foals. | 2009 | 19681725 |
| selective adsorption of hydrocarbon-oxidizing rhodococcus cells in a column with hydrophobized poly(acrylamide) cryogel. | a method for selective adsorption of rhodococcus cells in the column with hydrophobized poly(acrylamide) cryogel (cryopaag) was developed that allowed rhodococci separation from mixed bacterial populations and their effective concentration within a sponge-like gel matrix. hydrophobization of cryopaag using the n-dodecane graft (c12) was performed to enhance the adhesion of rhodococcus cells to the cryogel; this was suggested by our finding that alkanotrophic rhodococci possess high adhesive acti ... | 2009 | 19682505 |
| vertical distribution and anaerobic biodegradation of polycyclic aromatic hydrocarbons in mangrove sediments in hong kong, south china. | the vertical distribution of polycyclic aromatic hydrocarbons (pahs) at different sediment depths, namely 0-2 cm, 2-4 cm, 4-6 cm, 6-10 cm, 10-15 cm and 15-20 cm, in one of the most contaminated mangrove swamps, ma wan, hong kong was investigated. it was the first time to study the intrinsic potential of deep sediment to biodegrade pahs under anaerobic conditions and the abundance of electron acceptors in sediment for anaerobic degradation. results showed that the total pahs concentrations (summa ... | 2009 | 19683792 |
| morphological, physiological, and molecular characterization of a newly isolated steroid-degrading actinomycete, identified as rhodococcus ruber strain chol-4. | the aerobic degradation of cholesterol, testosterone, androsterone, progesterone, and further steroid compounds as sole carbon source has been observed in the newly isolated bacterial gram-positive strain chol-4. the 16s rrna gene sequence shares the greatest similarity with members of the genus rhodococcus, with the closest shared nucleotide identities of 98-99% with rhodococcus ruber (dsm 43338(t)) and rhodococcus aetherivorans (dsm 44752(t)). phylogenetic analysis of rhodococcus 16s rrna gene ... | 2009 | 19688376 |
| activation of foal neutrophils at different ages by cpg oligodeoxynucleotides and rhodococcus equi. | toll-like receptor 9 (tlr9) activation stimulates protective immune responses against intracellular pathogens by phagocytes, including neutrophils. this study examined tlr9-mediated neutrophil activation in neonatal foals. unmethylated cpgs, ligands for tlr9, were used to stimulate equine neutrophils, either purified or in contact with other peripheral blood leukocytes. rhodococcus equi was used as another stimulus in parallel. tlr9 mrna was constitutively expressed at a similar level in purifie ... | 2009 | 19819162 |
| methylcatechol 1,2-dioxygenase of rhodococcus opacus 6a is a new type of the catechol-cleaving enzyme. | the strains rhodococcus sp. 400, r. rhodochrous 172, and r. opacus 6a utilize 4-methylbenzoate as the only carbon and energy source. 4-methylcatechol is a key intermediate of biodegradation. its further conversion by all the strains proceeds via ortho-cleavage. the specific activity of catechol 1,2-dioxygenase assayed in crude extracts of rhodococcus sp. 400 and r. rhodochrous 172 with 3- and 4-methylcatechols does not exceed the enzyme activity assayed with catechol. two catechol 1,2-dioxygenas ... | 2009 | 19916910 |
| application of the second rule of transient-state kinetic isotope effects to an enzymatic mechanism. | the transient-state kinetic approach reveals the formation and subsequent interconversions of intermediates in real time. its potential for the mechanistic resolution of enzymatic and other complex chemical mechanisms has been severely limited however by the lack of a rigorous and applicable theoretical basis in contrast to that of the less direct but soundly based algebraic algorithms of the steady-state approach. having recently established three rigorously derived fundamental "rules" of trans ... | 2009 | 19924903 |
| role of interfacial tensions in the translocation of rhodococcus erythropolis during growth in a two phase culture. | rhodococcus erythropolis pr4 is an alkane-degrading bacterium, which grows well in media containing high concentrations of alkanes. these properties give the organism potential in the bioremediation of various environments contaminated by alkanes. in this study, we report the translocation of r. erythropolis pr4 from an aqueous phase to an alkane phase during growth in a two phase culture medium. when the alkane chain length was between c10 and c12, pr4 was located at the aqueous-alkane interfac ... | 2009 | 19924958 |
| 16s rrna gene-based identification of cultured bacterial flora from host-seeking ixodes ricinus, dermacentor reticulatus and haemaphysalis concinna ticks, vectors of vertebrate pathogens. | a total of 151 bacterial isolates were recovered from different developmental stages (larvae, nymphs and adults) of field-collected ticks (67 strains from ixodes ricinus, 38 from dermacentor reticulatus, 46 from haemaphysalis concinna). microorganisms were identified by means of 16s rrna gene sequencing. almost 87 % of the strains belonged to g(+) bacteria with predominantly occurring genera bacillus and paenibacillus. other g(+) strains included arthrobacter, corynebacterium, frigoribacterium, ... | 2009 | 19937215 |
| [promoter recognition and beta-galactosidase reporter gene expression in rhodococcus]. | the genus rhodococcus is of considerable interest in recent years, stemming from their diverse applications in biodegradation, bioremediation, biotransformation and biosurfactant. using nocardia/rhodococcus-escherichia coli shuttle plasmid pnv18.1 as the backbone vector, we tested the driven efficiency of promoters ptac and placz of e. coli and pami-1/pami-2 of r. ruber in host r. rhodochrous atcc 33278 by overexpression of nitrile hydratase. results showed that the specific activity of nitrile ... | 2009 | 19938479 |
| [particularities of alkane oxidation in rhodococcus erythropolis ek-1 strain--producer of surface-active substances]. | the hexadecane oxidation of rhodococcus erythropolls ek-1 was established to be catalyzed by the alkane hydroxylase complex containing fe-s proteid rubredoxin. alkane hydroxylase activity was increased twice in the presence of 10 mg/l iron ions in the cultivation medium. sodium ions were shown to be an activator of alkane hydroxylase. four types of alcohol dehydrogenases (nad, nadp, pyrroloquinoline quinone and n,n-dimethyll-4-nitrosoaniline-dependent enzymes) were found in hexadecane-grown cell ... | 2009 | 19938610 |
| the enhancement of biodesulfurization activity in a novel indigenous engineered pseudomonas putida. | the combustion of sulfur-rich fossil fuels leads to release of sulfur oxide pollution in the environment. in biodesulfurization process, an organism is able to remove sulfur from fossil fuels without decreasing the caloric value of those substrates. the main aim of this research was to design a recombinant microorganism to remove the highest amount of sulfur compounds in fossil fuels. | 2009 | 19946346 |
| bacteremia due to rhodococcus equi: a case report and review of the literature. | rhodococcus equi, previously known as corynebacterium equi, is one of the most important causes of zoonotic infections in grazing animals. increased cases of human infection with r. equi have been reported, especially in immunocompromised patients, within recent years. we present a case of r. equi bacteremia in a 51-year-old man with diabetes and liver cirrhosis, on long-term corticosteroid therapy after skin-grafting surgery. the patient recovered soon after he was treated with vancomycin. this ... | 2009 | 19946958 |
| disseminated rhodococcus equi infection in a kidney transplant patient without initial pulmonary involvement. | rhodococcus equi is increasingly recognized as an opportunistic pathogen in solid organ transplant recipients. primary pulmonary involvement is the most common finding. we report a case of a 42-year-old female kidney transplant recipient who developed multiple disseminated abscesses caused by r. equi while on adequate antimicrobial therapy. the patient presented with subcutaneous abscesses in the hip region and mamma and had 2 intracerebral abscesses. there were no clinical and radiologic signs ... | 2009 | 19762194 |
| [the introduction of the 9alpha-hydroxy group into androst-4-en-3,17-dione using a new actinobacterium strain]. | a 9alpha-hydrolase activity of a new actinobacterium strain identified as rhodococcus erythropolis based on the analysis of a 16s rrna gene sequence (1417 nucleotides) towards androst-4-en-3,17-dione (ad) was studied. in the presence of glucose in the medium, this strain completely transformed ad (4-20 g/l) into 9alpha-hydroxy-ad over 20-48 h. this culture was able to grow and perform ad 9alphahydroxylation at a concentration of dimethyl formamide up to 9%. crystalline 9alpha-hydroxy-ad was isol ... | 2009 | 19764613 |
| hydroxylation and further oxidation of delta9-tetrahydrocannabinol by alkane-degrading bacteria. | the microbial biotransformation of delta(9)-tetrahydrocannabinol was investigated using a collection of 206 alkane-degrading strains. fifteen percent of these strains, mainly gram-positive strains from the genera rhodococcus, mycobacterium, gordonia, and dietzia, yielded more-polar derivatives. eight derivatives were produced on a mg scale, isolated, and purified, and their chemical structures were elucidated with the use of liquid chromatography-mass spectrometry, (1)h-nuclear magnetic resonanc ... | 2009 | 19767471 |
| polyhydroxyalkanoates production by actinobacteria isolated from soil. | polyhydroxyalkanoates (phas) are biodegradable and renewable polymers produced by a wide range of bacterial groups. new microbial bioprospection approaches have become an important way to find new pha producers and new synthesized polymers. over the past years, bacteria belonging to actinomycetes group have become known as pha producers, such as nocardia and rhodococcus species, kineosphaera limosa liu et al. 2002, and, more recently, streptomyces species. in this paper, we disclose that there a ... | 2009 | 19767851 |
| aromatic hydrocarbon degradation genes from chronically polluted subantarctic marine sediments. | the goal of this study was to identify functional targets to detect polycyclic aromatic hydrocarbon (pah)-degrading bacterial populations in cold marine ecosystems. | 2009 | 19780966 |
| extrapulmonary disorders associated with rhodococcus equi infection in foals: 150 cases (1987-2007). | to describe frequency, types, and clinical outcomes of extrapulmonary disorders (epds) in foals in which rhodococcus equi infection was diagnosed, and to identify factors determined at the time of admission that differentiated foals that developed epds from foals with r equi infection identified only in the lungs. | 2009 | 19793018 |
| rhodococcus equi virulence-associated protein a is required for diversion of phagosome biogenesis but not for cytotoxicity. | rhodococcus equi is a gram-positive facultative intracellular pathogen that can cause severe bronchopneumonia in foals and aids patients. virulence is plasmid regulated and is accompanied by phagosome maturation arrest and host cell necrosis. a replacement mutant in the gene for vapa (virulence-associated protein a), a major virulence factor of r. equi, was tested for its activities during macrophage infection. early in infection, phagosomes containing the vapa mutant did not fuse with lysosomes ... | 2009 | 19797071 |
| [bacterial biodiversity in the groundwater contaminated by oil]. | an oil-contaminated groundwater sample was collected at an abandoned petrochemical factory.bacterial genome dna was extracted for the 16s rdna gene amplification,and then a bacterial 16s rdna gene clone library was constructed. after the phylogenetic analysis of 16s rdna sequences, bacterial diversity and community structure of the groundwater were studied. the results showed the bacteria in the groundwater could be divided into 10 groups, which were as follows: gamma-proteobacteria (49.1%), alp ... | 2009 | 19799318 |
| lipase diversity in glacier soil based on analysis of metagenomic dna fragments and cell culture. | lipase diversity in glacier soil was assessed by culture independent metagenomic dna fragment screening and confirmed by cell culture experiments. a set of degenerate pcr primers specific for lipases of the hormone-sensitive lipase family was designed based on conserved motifs and used to directly pcr amplify metagenomic dna from glacier soil. these products were used to construct a lipase fragment clone library. among the 300 clones sequenced for the analysis, 201 clones encoding partial lipase ... | 2009 | 19809244 |
| immunohistological demonstration of rhodococcus equi in a trotter foal. | a 3-month-old female trotter foal was euthanized due to severe dyspnoea. pathomorphologically a chronic granulomatous to necrotizing pneumonia was found and rhodoccocus (r.) equi was isolated microbiologically. an immunohistological method using a murine monoclonal antibody against a 15-17 kda antigen of virulent r. equi was established in formalin-fixed and paraffin-embedded tissue sections using various antigen retrieval techniques to optimize the staining results. microwave treatment was most ... | 2009 | 19813451 |
| isolation and characterization of an atrazine-degrading rhodococcus sp. strain mb-p1 from contaminated soil. | the aim of this study is to isolate and characterize organisms capable of utilizing high concentration atrazine from the contaminated sites. | 2009 | 19818008 |
| degradation of a mixture of hydrocarbons, gasoline, and diesel oil additives by rhodococcus aetherivorans and rhodococcus wratislaviensis. | two strains, identified as rhodococcus wratislaviensis ifp 2016 and rhodococcus aetherivorans ifp 2017, were isolated from a microbial consortium that degraded 15 petroleum compounds or additives when provided in a mixture containing 16 compounds (benzene, toluene, ethylbenzene, m-xylene, p-xylene, o-xylene, octane, hexadecane, 2,2,4-trimethylpentane [isooctane], cyclohexane, cyclohexanol, naphthalene, methyl tert-butyl ether [mtbe], ethyl tert-butyl ether [etbe], tert-butyl alcohol [tba], and 2 ... | 2009 | 19837842 |
| cytochrome p450 125 (cyp125) catalyses c26-hydroxylation to initiate sterol side-chain degradation in rhodococcus jostii rha1. | the cyp125 gene of rhodococcus jostii rha1 was previously found to be highly upregulated during growth on cholesterol and the orthologue in mycobacterium tuberculosis (rv3545c) has been implicated in pathogenesis. here we show that cyp125 is essential for r. jostii rha1 to grow on 3-hydroxysterols such as cholesterol, but not on 3-oxo sterol derivatives, and that cyp125 performs an obligate first step in cholesterol degradation. the involvement of cyp125 in sterol side-chain degradation was conf ... | 2009 | 19843222 |
| mycobacterial cytochrome p450 125 (cyp125) catalyzes the terminal hydroxylation of c27 steroids. | cyp125 (rv3545c), a cytochrome p450, is encoded as part of the cholesterol degradation gene cluster conserved among members of the mycobacterium tuberculosis complex. this enzyme has been implicated in mycobacterial pathogenesis, and a homologue initiates cholesterol catabolism in the soil actinomycete rhodococcus jostii rha1. in mycobacterium bovis bcg, cyp125 was up-regulated 7.1-fold with growth on cholesterol. a cyp125 deletion mutant of bcg did not grow on cholesterol and accumulated 4-chol ... | 2009 | 19846551 |
| a polyphasic study on the species diversity of the sediment microbiota of lake hévíz. | lake hévíz is the largest natural thermal lake of europe, harboring special bacterial communities. the aim of the present study was to gain information about the distribution and species diversity of the sediment microbiota, with special focus on actinobacteria, by using cultivation-based and -independent molecular methods. samples from two depths were taken in two different locations in october 2007. 245 strains were isolated, grouped to 85 otus by ardra, and identified by 16s rdna sequencing. ... | 2009 | 20038486 |
| analysis of the dibenzothiophene metabolic pathway in a newly isolated rhodococcus spp. | out of 17 samples collected from diverse environments, 110 bacterial isolates of varied characteristics were screened for their dibenzothiophene-desulphurizing activity. a single isolate, eu-32, originating from a soil sample taken from the roots of a eucalyptus tree, displayed dibenzothiophene-desulphurizing activity. this isolate metabolized dibenzothiophene to 2-hydroxybiphenyl (2-hbp), as detected by hplc, and was also able to use other organic sulphur compounds as a sole sulphur source. bas ... | 2009 | 19824901 |
| rhodococcus equi infection in a surgical wound. | a 35-year-old male presented with abdominal pain one month after receiving a routine ventral hernia repair. over the course of two months, repeated wound cultures were ordered and eventually produced growth of rhodococcus equi. appropriate antibacterial therapy was initiated to resolve the infection. | 2009 | 19827407 |
| [rhodococcus equi neumonia in a hiv infected patient: a case report]. | 2009 | 20067738 | |
| inhibitory effect of aged-petroleum hydrocarbons on the survival of inoculated microorganism in a crude-oil contaminated site. | we studied the effects of aged total petroleum hydrocarbons (aged tph) on the survival of allochthonous diesel-degrading rhodococcus sp. strain ys-7 in both laboratory and field investigations. the aged tph extracted from a crude oil-contaminated site were fractionized by thin-layer chromatography/flame ionization detection (tlc/fid). the three fractions identified were saturated aliphatic (sa), aromatic hydrocarbon (ah), and asphaltene-resin (ar). the ratio and composition of the separated frac ... | 2009 | 20075636 |
| [screening and denitrification characteristics of a heterotrophic nitrification-aerobic denitrifier bacteria]. | a heterotrophic nitrification-aerobic denitrifier bacteria cpz24 was isolated form the livestock wastewater by way of the limiting dilution combined with the chromogenic medium screening methods. this bacterium was gram positive, rod. the colonies of the strain were orange-red.it was identified as rhodococuus pyridinivorans according to its morphological and physiological properties and the analysis of its 16s rdna gene. studied on its function of heterotrophic nitrification and aerobic denitrif ... | 2009 | 20187396 |
| [cloning, heterologous expression and purification of a 3-ketosteroid-9alpha-hydroxylase (ksh) from mycobacterium sp. nwib-01]. | 3-ketosteroid-9alpha-hydroxylase (ksh), a key enzyme in the microbial steroid degradation, is highly valuable for the production of some steroid drugs. degenerate primers were designed by comparing the ksh from rhodococcus erythropolis sq1 and its homologous sequences in the reported genome of mycobacteria. subsequently, a gene fragment of ksh was cloned from mycobacterium sp. nwib-01, a sterol-transforming bacterium isolated from soil in our lab. according to the conservative sequence, the full ... | 2009 | 20352983 |
| functional expression of three rieske non-heme iron oxygenases derived from actinomycetes in rhodococcus species for investigation of their degradation capabilities of dibenzofuran and chlorinated dioxins. | the activity of rieske non-heme iron oxygenases (aromatic hydrocarbon dioxygenases, ahdos) is important for the bacterial degradation of aromatic pollutants such as polycyclic aromatic hydrocarbons and dioxins. during our analysis of the role of ahdos in dioxin bioremediation, some enzymes derived from high g + c gram-positive actinomycetes were difficult to produce in active form in the escherichia coli protein expression system. in this study, we constructed a heterologous expression system fo ... | 2009 | 19352040 |
| insights into sequence-activity relationships amongst baeyer-villiger monooxygenases as revealed by the intragenomic complement of enzymes from rhodococcus jostii rha1. | microbial genome sequences are providing a wealth of information on new enzymes that have considerable potential as biocatalysts. the recently sequenced genome of rhodococcus jostii rha1, for example, has revealed an impressive array of catabolic enzymes, including many putative baeyer-villiger monooxygenases (bvmos). we have cloned 23 target bvmo sequences from the genome of r. jostii rha1 and heterologously expressed 13 of these as soluble proteins to unearth new substrate specificities and se ... | 2009 | 19360806 |
| the clinical importance of microbiological findings in the diagnosis and management of bloodstream infections. | bloodstream infections are associated with high morbidity and mortality. accurate identification of blood isolates to the species level and identification of the source of infection and/or the portal of entry are crucial for optimal management of these infections. these investigations-in addition to clinical findings and laboratory and imaging studies-are central to informing and directing efficient and effective diagnostic examinations and to choosing the optimal antimicrobial regimen. four cas ... | 2009 | 19374579 |
| a previously uncultured, paper mill propionibacterium is able to degrade o-aryl alkyl ethers and various aromatic hydrocarbons. | a previously uncultured propionibacterium was isolated from a highly diluted sample (10(-6)ml) of activated sludge of paper mill effluent. the isolate mob600 was able to grow on anisole, phenetole, benzene, toluene, phenol, styrene and biphenyl, although it used only limited carbon sources in the minimal media. the partial dna sequence of 16s ribosomal rna gene was 93% identical to luteococcus peritoni ccug38120 as the closest neighborhood in the family propionibacteriaceae. strain mob600 produc ... | 2009 | 19375147 |
| bacterial communities from shoreline environments (costa da morte, northwestern spain) affected by the prestige oil spill. | the bacterial communities in two different shoreline matrices, rocks and sand, from the costa da morte, northwestern spain, were investigated 12 months after being affected by the prestige oil spill. culture-based and culture-independent approaches were used to compare the bacterial diversity present in these environments with that at a nonoiled site. a long-term effect of fuel on the microbial communities in the oiled sand and rock was suggested by the higher proportion of alkane and polyaromat ... | 2009 | 19376924 |
| linear b-cell epitope mapping using enzyme-linked immunosorbent assay for libraries of overlapping synthetic peptides. | the aim of this chapter is to provide a strategy for mapping linear antibody epitopes of protein antigens in order to discover candidates for vaccines or diagnostic tests. a set of overlapping peptides was designed and synthesised based upon a known amino acid sequence of the target protein, virulence-associated protein a (vapa) of the bacterium rhodococcus equi, an important pulmonary pathogen in foals.the peptides were biotinylated and used in an elisa to screen immune sera from foals. these b ... | 2009 | 19377942 |
| [phenanthrene and anthracene degradation by microorganisms of the genus rhodococcus]. | the cells of rhodococcus opacus 412 and r. rhodnii 135 were adapted to phenanthrene and anthracene on a solid mineral medium. preliminary adaptation of the strains accelerated the metabolism of polyaromatic hydrocarbons and provided for the ability of microorganisms to grow on pheanthrene as a sole carbon and energy source in a liquid mineral medium. it was shown that phenanthrene was mineralized by the strains through 7,8-benzocoumarin, 1-hydroxy-2-naphthoaldehyde, 1-hydroxy-2-naphthoic acid, s ... | 2009 | 19382706 |
| crystal structures of cyclohexanone monooxygenase reveal complex domain movements and a sliding cofactor. | cyclohexanone monooxygenase (chmo) is a flavoprotein that carries out the archetypical baeyer-villiger oxidation of a variety of cyclic ketones into lactones. using nadph and o(2) as cosubstrates, the enzyme inserts one atom of oxygen into the substrate in a complex catalytic mechanism that involves the formation of a flavin-peroxide and criegee intermediate. we present here the atomic structures of chmo from an environmental rhodococcus strain bound with fad and nadp(+) in two distinct states, ... | 2009 | 19385644 |
| laboratory-scale biofiltration of acrylonitrile by rhodococcus rhodochrous dap 96622 in a trickling bed bioreactor. | acrylonitrile (acn), a volatile component of the waste generated during the production of acrylamide, also is often associated with aromatic contaminants such as toluene and styrene. biofiltration, considered an effective technique for the treatment of volatile hydrocarbons, has not been used to treat volatile nitriles. an experimental laboratory-scale trickling bed bioreactor using cells of rhodococcus rhodochrous dap 96622 supported on granular activated carbon (gac) was developed and evaluate ... | 2009 | 19387713 |
| mechanism of membrane permeabilization by a bacterial trehalose lipid biosurfactant produced by rhodococcus sp. | the interactions of a succinoyl bacterial trehalose lipid biosurfactant produced by rhodococcus sp. with phospholipid vesicles, leading to membrane permeabilization, are studied by means of calorimetric and fluorescence and absorption spectroscopical techniques in search for a molecular model. the critical micelle concentration (cmc) of trehalose lipid is determined, by surface tension measurements, to be 300 mum. binding of trehalose lipid to palmitoyloleoylphosphatidylcholine membranes is stud ... | 2009 | 19391573 |
| the physicochemical properties and chemical composition of trehalose lipids produced by rhodococcus erythropolis 51t7. | this study analyzed the chemical and physical properties of a biosurfactant synthesized by rhodococcus sp. 51t7. the biosurfactant was a trehalose tetraester (thl) consisting of six components: one major and five minor. the hydrophobic moieties ranged in size from 9 to 11 carbons. the critical micelle concentration (cmc) was 0.037g l(-1) and the interfacial tension against hexadecane was 5mn m(-1). at ph 7.4 the glycolipid cmc/critical aggregation concentration (cac) was 0.05g l(-1) and at ph 4 ... | 2009 | 19428355 |
| biodegradation aspects of polycyclic aromatic hydrocarbons (pahs): a review. | pahs are aromatic hydrocarbons with two or more fused benzene rings with natural as well as anthropogenic sources. they are widely distributed environmental contaminants that have detrimental biological effects, toxicity, mutagenecity and carcinogenicity. due to their ubiquitous occurrence, recalcitrance, bioaccumulation potential and carcinogenic activity, the pahs have gathered significant environmental concern. although pah may undergo adsorption, volatilization, photolysis, and chemical degr ... | 2009 | 19442441 |
| purification, characterization, and directed evolution study of a vitamin d3 hydroxylase from pseudonocardia autotrophica. | vitamin d(3) (vd(3)) is a fat-soluble prohormone that plays a crucial role in bone metabolism, immunity, and control of cell proliferation and cell differentiation in mammals. the actinomycete pseudonocardia autotrophica is capable of bioconversion of vd(3) into its physiologically active forms, namely, 25(oh)vd(3) or 1alpha,25(oh)(2)vd(3). in this study, we isolated and characterized vdh (vitamin d(3) hydroxylase), which hydroxylates vd(3) from p. autotrophica nbrc 12743. the vdh gene encodes a ... | 2009 | 19450562 |
| influence of extracellular polymeric substances (eps) on deposition kinetics of bacteria. | the significance of extracellular polymer substances (eps) on cell deposition on silica surfaces was examined by direct comparison of the deposition kinetics of untreated "intact" bacteria versus those from the same strain but with eps removal via cation exchange resin (cer) treatment using a quartz crystal microbalance with dissipation (qcm-d). four bacterial strains, mutant escherichia coli bl21 (gram-negative, nonmotile), pseudomonas sp qg6 (gram-negative, motile), rhodococcus sp ql2 (gram-po ... | 2009 | 19452879 |
| identification of hexahydro-1,3,5-trinitro-1,3,5-triazine-degrading microorganisms via 15n-stable isotope probing. | this study reported the application of 15n-stable isotope probing (sip) to identify active hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx)-utilizing microorganisms in groundwater microcosms. fifteen 16s rrna gene sequences were derived from the 15n-dna fraction (contributed from active microorganisms capable of using rdx as a nitrogen source) of microcosms receiving cheese whey. the 16s rrna gene sequences belonged to actinobacteria (two clones), alpha-proteobacteria (seven clones), and gamma-prot ... | 2009 | 19452908 |
| molecular and infection biology of the horse pathogen rhodococcus equi. | the soil actinomycete rhodococcus equi is a pulmonary pathogen of young horses and aids patients. as a facultative intracellular bacterium, r. equi survives and multiplies in macrophages and establishes its specific niche inside the host cell. recent research into chromosomal virulence factors and into the role of virulence plasmids in infection and host tropism has presented novel aspects of r. equi infection biology and pathogenicity. this review will focus on new findings in r. equi biology, ... | 2009 | 19453748 |
| case report: rhodococcus erythropolis osteomyelitis in the toe. | the rhodococcus species rarely cause musculoskeletal infections, with only two cases reported in the literature. we report the case of a 53-year-old woman who had an infection develop after first metatarsophalangeal joint fusion. a year after surgery, she continued to have pain and swelling with nonunion. she underwent revision of the arthrodesis and tissue samples from surgery revealed rhodococcus erythropolis. the patient's symptoms improved with oral antibiotics. one year after the revision s ... | 2009 | 19459020 |
| a new fluorescent oligonucleotide probe for in situ detection of s-triazine-degrading rhodococcus wratislaviensis in contaminated groundwater and soil samples. | a bacterial strain (fpa1) capable of using terbuthylazine, simazine, atrazine, 2-hydroxysimazine, deethylatrazine, isopropylamine or ethylamine as its sole carbon source was isolated from a shallow aquifer chronically contaminated with s-triazine herbicides. based on its 16s rdna sequence analysis, the strain fpa1 was identified as rhodococcus wratislaviensis. the disappearance time of 50% of the initial terbuthylazine concentration in the presence of this strain (dt(50)) was 62days. this strain ... | 2009 | 19476963 |
| measuring 'hydrophobicity' of filamentous bacteria found in wastewater treatment plants. | attempts to measure the hydrophobicity of the cell surfaces of gordonia amarae and rhodococcus erythropolis, filamentous bacteria found in wastewater treatment plants, by several methods--microbial adhesion to hydrocarbons (math) or bacterial adhesion to hydrocarbons (bath), contact angle, and micro-sphere adhesion to cells (mac)--were unsuccessful. the results were erratic and inconsistent. this was in part because of the filamentous growth habit of g. amarae, but it was also a consequence of t ... | 2009 | 19477105 |
| structure and activity of the n-terminal substrate recognition domains in proteasomal atpases. | the proteasome forms the core of the protein quality control system in archaea and eukaryotes and also occurs in one bacterial lineage, the actinobacteria. access to its proteolytic compartment is controlled by aaa atpases, whose n-terminal domains (n domains) are thought to mediate substrate recognition. the n domains of an archaeal proteasomal atpase, archaeoglobus fulgidus pan, and of its actinobacterial homolog, rhodococcus erythropolis arc, form hexameric rings, whose subunits consist of an ... | 2009 | 19481487 |
| identification of a novel self-sufficient styrene monooxygenase from rhodococcus opacus 1cp. | sequence analysis of a 9-kb genomic fragment of the actinobacterium rhodococcus opacus 1cp led to identification of an open reading frame encoding a novel fusion protein, stya2b, with a putative function in styrene metabolism via styrene oxide and phenylacetic acid. gene cluster analysis indicated that the highly related fusion proteins of nocardia farcinica ifm10152 and arthrobacter aurescens tc1 are involved in a similar physiological process. whereas 413 amino acids of the n terminus of stya2 ... | 2009 | 19482928 |
| isolation and characterization of 3-n-trimethylamino-1-propanol-degrading rhodococcus sp. strain a2. | the aerobic degradation of 3-n-trimethylamino-1-propanol (homocholine) as a sole source of carbon and nitrogen has been found for a rhodococcus sp. bacterium isolated from soil. the isolate was identified as rhodococcus sp. strain a2 based on its phenotypic features, physiological and biochemical characteristics, and results of phylogenetic analysis. the washed cells of strain a2 completely degraded homocholine within 6 h, with concomitant formation of several metabolites. analysis of the metabo ... | 2009 | 19486158 |
| first case of bloodstream infection caused by rhodococcus erythropolis. | we describe the first case of bloodstream infection caused by rhodococcus erythropolis. the identification was performed using 16s rrna sequencing. this case illustrates that non-equi rhodococcus infections may be underdiagnosed due to difficulties in identification in the routine clinical microbiology laboratory. | 2009 | 19494072 |
| production of 3-hydroxypropionic acid from acrylic acid by newly isolated rhodococcus erythropolis lg12. | a novel microorganism, designated as lg12, was isolated from soil based on its ability to use acrylic acid as the sole carbon source. an electron microscopic analysis of its morphological characteristics and phylogenetic classification by 16s rrna homology showed that the lg12 strain belongs to rhodococcus erythropolis. r. erythropolis lg12 was able to metabolize a high concentration of acrylic acid (up to 40 g/l). in addition, r. erythropolis lg12 exhibited the highest acrylic acid-degrading ac ... | 2009 | 19494695 |
| synergistic effect of carbapenem-teicoplanin combination during severe rhodococcus equi pneumonia in a kidney transplant recipient. | rhodococcus equi is a bacterial pathogen of domestic animals that can infect immunocompromised patients, especially those with impaired cellular immunity, such as transplant recipients. no standard treatment has been established, but therapy must be prolonged, as relapses are common and can occur at the initial site or distant locations. here we report a case of r. equi-associated pulmonary abscess in a renal transplant recipient successfully treated with a combination of carbapenem and teicopla ... | 2009 | 19497046 |
| designer enzymes for glycosphingolipid synthesis by directed evolution. | though glycosphingolipids have great potential as therapeutics for cancer, hiv, neurodegenerative diseases and auto-immune diseases, both extensive study of their biological roles and development as pharmaceuticals are limited by difficulties in their synthesis, especially on large scales. here we addressed this restriction by expanding the synthetic scope of a glycosphingolipid-synthesizing enzyme through a combination of rational mutagenesis and directed evolution with an elisa-based screening ... | 2009 | 19525967 |
| engineering and improvement of the efficiency of a chimeric [p450cam-rhfred reductase domain] enzyme. | a chimeric oxygenase, in which the p450cam domain was fused to the reductase host domains of a p450rhf from rhodococcus sp. strain ncimb 9784 was optimised to allow for a biotransformation at 30 mm substrate in 80% overall yield, with the linker region between p450 and fmn domain proving to be important for the effective biotransformation of (+)-camphor to 5-exo-hydroxycamphor. | 2009 | 19532862 |
| in situ magnetic separation and immobilization of dibenzothiophene-desulfurizing bacteria. | in situ cell separation and immobilization of bacterial cells for biodesulfurization were developed by using superparamagnetic fe(3)o(4) nanoparticles (nps). the fe(3)o(4) nps were synthesized by coprecipitation followed by modification with ammonium oleate. the surface-modified nps were monodispersed and the particle size was about 13 nm with 50.8 emu/g saturation magnetization. after adding the magnetic fluids to the culture broth, rhodococcus erythropolis lsse8-1 cells were immobilized by ads ... | 2009 | 19541480 |
| aerobic biodegradation of n-nitrosodimethylamine by the propanotroph rhodococcus ruber env425. | the propanotroph rhodococcus ruber env425 was observed to rapidly biodegrade n-nitrosodimethylamine (ndma) after growth on propane, tryptic soy broth, or glucose. the key degradation intermediates were methylamine, nitric oxide, nitrite, nitrate, and formate. small quantities of formaldehyde and dimethylamine were also detected. a denitrosation reaction, initiated by hydrogen atom abstraction from one of the two methyl groups, is hypothesized to result in the formation of n-methylformaldimine an ... | 2009 | 19542346 |
| non mycobacterial virulence genes in the genome of the emerging pathogen mycobacterium abscessus. | mycobacterium abscessus is an emerging rapidly growing mycobacterium (rgm) causing a pseudotuberculous lung disease to which patients with cystic fibrosis (cf) are particularly susceptible. we report here its complete genome sequence. the genome of m. abscessus (cip 104536t) consists of a 5,067,172-bp circular chromosome including 4920 predicted coding sequences (cds), an 81-kb full-length prophage and 5 is elements, and a 23-kb mercury resistance plasmid almost identical to pmm23 from mycobacte ... | 2009 | 19543527 |
| evaluation of antibacterial activity, phenol and flavonoid contents of thespesia populnea flower extracts. | flavonoids which were reported as having many pharmacological activities, antimicrobial, antioxidant, cytotoxic, chemoprevention activities and they possess strong antiproliferative effects related to inhibition of cell cycle progression and apoptosis induction. on the basis of this thespesia populnea (l.) sol. ex correa (family-malvaceae) was selected and it is having the major composition of flavonoids and the antibacterial activity of methanolic extract of thespesia populnea flowers was inves ... | 2009 | 19553175 |
| continuous hydrolysis of 4-cyanopyridine by nitrilases from fusarium solani o1 and aspergillus niger k10. | the operational stabilities of nitrilases from aspergillus niger k10 and fusarium solani o1 were examined with 4-cyanopyridine as the substrate in continuous-stirred membrane reactors (csmrs). the former enzyme was fairly stable at 30 degrees c with a deactivation constant (k (d)) and enzyme half-life of 0.014 h(-1) and 50 h, respectively, but the latter exhibited an even higher stability characterized by k (d) = 0.008 h(-1) and half-life of 87 h at 40 degrees c. another advantage of this enzyme ... | 2009 | 19554325 |
| rhodococcus rhodochrous dsm 43269 3-ketosteroid 9alpha-hydroxylase, a two-component iron-sulfur-containing monooxygenase with subtle steroid substrate specificity. | this paper reports the biochemical characterization of a purified and reconstituted two-component 3-ketosteroid 9alpha-hydroxylase (ksh). ksh of rhodococcus rhodochrous dsm 43269, consisting of a ferredoxin reductase (kshb) and a terminal oxygenase (ksha), was heterologously expressed in escherichia coli. e. coli cell cultures, expressing both ksha and kshb, converted 4-androstene-3,17-dione (ad) into 9alpha-hydroxy-4-ad (9ohad) with a >60% molar yield over 48 h of incubation. coexpression and c ... | 2009 | 19561185 |
| occupational exposure to organic dust, microorganisms, endotoxin and peptidoglycan among plants processing workers in poland. | the objective of present work was to determine and compare the components of bioaerosol in several sectors of plant processing industries. the study was conducted in 10 facilities engaged in herb and grain processing, flax threshing, grain storing, baking, and cereals production. the air samples were taken on glass fibre filters with an as-50 sampler. we determined the concentrations of airborne microorganisms, dust, endotoxin and peptidoglycan. total concentrations of viable airborne microorgan ... | 2009 | 19572487 |
| isolation, identification and characterization of a novel rhodococcus sp. strain in biodegradation of tetrahydrofuran and its medium optimization using sequential statistics-based experimental designs. | statistics-based experimental designs were applied to optimize the culture conditions for tetrahydrofuran (thf) degradation by a newly isolated rhodococcus sp. yyl that tolerates high thf concentrations. single factor experiments were undertaken for determining the optimum range of each of four factors (initial ph and concentrations of k(2)hpo(4).3h(2)o, nh(4)cl and yeast extract) and these factors were subsequently optimized using the response surface methodology. the plackett-burman design was ... | 2009 | 19230656 |
| [phenol degradation by rhodococcus opacus strain 1g]. | during cultivation in a liquid medium, the bacterium rhodococcus opacus 1g was capable of growing on phenol at a concentration of up to 0.75 g/l. immobilization of rhodococcus opacus 1g had a positive effect on cell growth in the presence of phenol at high concentrations. the substrate at concentrations of 1.0 and 1.5 g/l was completely utilized over 24 and 48 h, respectively. the key enzymes of phenol degradation (two pyrocatechol 1,2-dioxygenases and muconate cycloisomerase) were isolated. one ... | 2009 | 19235509 |
| malacoplakia of the tongue: a case report and clinicopathologic review of 6 cases. | extra-urogenital tract malacoplakia is uncommon, with tongue malacoplakia being exceptionally rare. the nonspecific clinical presentation and variable histologic patterns can make recognition of this lesion and separation from other lesions challenging. there are only a few reported cases in the english literature. | 2009 | 19239951 |
| synergistic and antagonistic hemolytic activities of bacteria of genus arcanobacterium and camp-like hemolysis of arcanobacterium phocae and arcanobacterium haemolyticum with psychrobacter phenylpyruvicus. | a total of 57 bacteria representing eight species of genus arcanobacterium (a.) were investigated for hemolytic properties on blood agar containing sheep and rabbit blood and for camp-like reactions. an enhanced hemolysis on blood agar containing rabbit blood compared to sheep blood could be observed for a. haemolyticum, less pronounced for a. hippocoleae and a. pluranimalium. a synergistic hemolytic reaction with staphylococcal beta-hemolysin appeared to be constantly visible for a. hippocoleae ... | 2009 | 19249067 |
| lateral transfer of genes for hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) degradation. | recent studies demonstrated that degradation of the military explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) by species of rhodococcus, gordonia, and williamsia is mediated by a novel cytochrome p450 with a fused flavodoxin reductase domain (xpla) in conjunction with a flavodoxin reductase (xplb). pulse field gel analysis was used to localize xpla to extrachromosomal elements in a rhodococcus sp. and distantly related microbacterium sp. strain ma1. comparison of rhodococcus rhodochrous 1 ... | 2009 | 19270122 |
| respiratory infections in immunocompromised patients. | immunosuppressive states and therapies are becoming common in clinical practice. recent advances and trends in bacterial, fungal, viral and parasitic pulmonary infections in immunosuppressed patients are described. | 2009 | 19276812 |
| media optimization for biosurfactant production by rhodococcus erythropolis mtcc 2794: artificial intelligence versus a statistical approach. | this paper entails a comprehensive study on production of a biosurfactant from rhodococcus erythropolis mtcc 2794. two optimization techniques--(1) artificial neural network (ann) coupled with genetic algorithm (ga) and (2) response surface methodology (rsm)--were used for media optimization in order to enhance the biosurfactant yield by rhodococcus erythropolis mtcc 2794. ann and rsm models were developed, incorporating the quantity of four medium components (sucrose, yeast extract, meat pepton ... | 2009 | 19283419 |
| the influence of age and rhodococcus equi infection on cd1 expression by equine antigen presenting cells. | there is a distinct age-associated susceptibility of horses to rhodococcus equi infection. initial infection is thought to occur in the neonatal and perinatal period, and only foals less than 6 months of age are typically affected. r. equi is closely related and structurally similar to mycobacterium tuberculosis, and causes similar pathologic lesions. protective immune responses to m. tuberculosis involve classical major histocompatibility complex (mhc)-restricted t cells that recognize peptide ... | 2009 | 19285733 |
| effect of substrate interaction on the degradation of methyl tert-butyl ether, benzene, toluene, ethylbenzene, and xylene by rhodococcus sp. | it was examined the substrate interactions of benzene (b), tolulene (t), ethylbenzene (e), xylene (x), and methyl tert-butyl ether (m) in binary, ternary, quaternary, and quinary mixtures by rhodococcus sp. eh831 that could aerobically degrade all of five single components. the specific degradation rates (sdrs) of b, t, e, x, and m were 234, 913, 131, 184 and 139 micromol g-dry cell weight (dcw)(-1)h(-1), respectively. in binary, ternary, quaternary, and quinary mixtures of them, ethylbenzene wa ... | 2009 | 19201538 |
| molecular characterization of rhodococcus equi from horse-breeding farms by means of multiplex pcr for the vap gene family. | this study evaluated the molecular characteristics of rhodococcus equi isolates obtained from horses by a multiplex pcr assay that amplifies the vap gene family (vapa, -b, -c, -d, -e, -f, -g, and -h). a total of 180 r. equi isolates were studied from four different sources, namely healthy horse feces (112), soil (12), stalls (23), and clinical isolates (33) from horse-breeding farms. the technique was performed and confirmed by sequencing of amplified vap gene family controls. thirty-two (17.8%) ... | 2009 | 19205798 |
| new alk genes detected in antarctic marine sediments. | alkane monooxygenases (alk) are the key enzymes for alkane degradation. in order to understand the dispersion and diversity of alk genes in antarctic marine environments, this study analysed by clone libraries the presence and diversity of alk genes (alkb and alkm) in sediments from admiralty bay, king george island, peninsula antarctica. the results show a differential distribution of alk genes between the sites, and the predominant presence of new alk genes, mainly in the pristine site. sequen ... | 2009 | 19207566 |
| antimicrobial properties of the equine alpha-defensin defa1 against bacterial horse pathogens. | defensins are small effector molecules of the innate immune system, synthesised by various organisms including plants and animals. the peptides act as endogenous antibiotics with an antimicrobial activity against a broad spectrum of microbes including bacteria, fungi and viruses. alpha-defensins are a subgroup of the defensin family, their synthesis is limited to some tissues and furthermore to some mammalian species including the horse. equine defa1 is an enteric alpha-defensin exclusively prod ... | 2009 | 19211153 |
| rhodococcus aetherivorans iar1, a new bacterial strain synthesizing poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from toluene. | toluene is the volatile organic compound (voc) that is most abundantly emitted to the environment. here, we report a new bacterial strain, iar1, which synthesizes polyhydroxyalkanoate (pha) from toluene as the sole carbon source. using physiological tests and phylogenetic analysis, iar1 was identified as rhodococcus aetherivorans. degradation of toluene by resting iar1 cells followed michaelis-menten kinetics. the determined kinetic parameters showed that the degradation ability of iar1 is sligh ... | 2009 | 19217552 |
| [pulmonary malacoplakia associated with rhodococcus equi infection in a patient with aids]. | 2009 | 19218010 | |
| metabolic shift in the phytopathogen rhodococcus fascians in response to cell-free extract of infected tobacco plant tissues. | the phytopathogen rhodococcus fascians induces the development of leafy gall, which is considered to be its ecological niche. to obtain a view of the metabolic changes occurring in r. fascians during this process, an in vitro system was used where bacteria are grown in the presence of a leafy gall extract, a condition mimicking that found by the bacteria in infected plants. proteins of r. fascians grown for 24 h under these conditions were displayed by two-dimensional polyacrylamide gel electrop ... | 2009 | 19219499 |
| enhanced production of amidase from rhodococcus erythropolis mtcc 1526 by medium optimisation using a statistical experimental design. | in the present work, statistical experimental methodology was used to enhance the production of amidase from rhodococcus erythropolis mtcc 1526. r. erythropolis mtcc 1526 was selected through screening of seven strains of rhodococcus species. the placket-burman screening experiments suggested that sorbitol as carbon source, yeast extract and meat peptone as nitrogen sources, and acetamide as amidase inducer are the most influential media components. the concentrations of these four media compone ... | 2009 | 19221820 |
| isolation and characterization of rhodococcus sp. y22 and its potential application to tobacco processing. | a novel nicotine-degrading bacterium, strain y22, was isolated and identified as rhodococcus sp. y22 based on its 16s rdna sequence and morphological and biochemical features. the isolate could utilize nicotine as the sole source of carbon and nitrogen. nicotine (1.0g/l) was degraded by rhodococcus sp. y22 within 52h at 28 degrees c and ph 7.0. preparation of resting cells from nicotine-induced cultures was found to rapidly and efficiently degrade nicotine from solutions as well as from tobacco ... | 2009 | 19298855 |
| rhodococcus equi comes of age. | 2009 | 19301589 | |
| the most-probable-number enumeration of dichlobenil and 2,6-dichlorobenzamide (bam) degrading microbes in finnish aquifers. | in groundwater subsurface deposits and a topsoil from five aquifers having 2,6-dichlorobenzamide (bam) in water, we determined the most-probable-number (mpn) of 2,6-dichlorobenzonitrile (dichlobenil) and metabolite bam degrading microorganisms. dichlobenil and bam were combined nitrogen sources in the mpn tubes, which were scored positive at concentrations <75% after 1 month incubation. aerobic and anaerobic microbes degrading dichlobenil and bam were common in samples in low numbers of 3.6-210 ... | 2009 | 19326247 |
| acute osteomyelitis caused by rhodococcus equi in an immunocompetent child. | rhodococcus equi is an unusual pathogen causing infections mostly in immunocompromised patients, particularly in those with human immunodeficiency virus (hiv). it has rarely been reported to affect immunocompetent hosts, where it usually presents as an isolated extrapulmonary lesion. we report a case of osteomyelitis caused by this organism in an immunocompetent host. | 2009 | 19332935 |
| analysis of force interactions between afm tips and hydrophobic bacteria using dlvo theory. | microbial adhesion to surfaces and interfaces is strongly influenced by their structure and physicochemical properties. we used atomic force microscopy (afm) to measure the forces between chemically functionalized afm tips and two bacterial species exhibiting different cell surface hydrophobicities, measured as the oil/water contact angle (theta): acinetobacter venetianus rag-1 (theta = 56.4 degrees ) and rhodococcus erythropolis 20s-e1-c (theta = 152.9 degrees ). the forces were measured as the ... | 2009 | 19334745 |
| biodegradation of dimethyl phthalate, diethyl phthalate and di-n-butyl phthalate by rhodococcus sp. l4 isolated from activated sludge. | in this study, an aerobic bacterial strain capable of utilizing dimethyl phthalate (dmp), diethyl phthalate (dep) and di-n-butyl phthalate (dbp) as sole carbon source and energy was isolated from activated sludge collected from a dyeing plant. according to its morphology, physiochemical characteristics and 16s rdna sequence, the strain was identified as rhodococcus ruber. the biodegradation batch tests of dmp, dep and dbp by the rhodococcus sp. l4 showed the optimal ph value, temperature and sub ... | 2009 | 19342169 |
| crystallization and preliminary x-ray diffraction studies of vitamin d3 hydroxylase, a novel cytochrome p450 isolated from pseudonocardia autotrophica. | vitamin d(3) hydroxylase (vdh) is a novel cytochrome p450 monooxygenase isolated from the actinomycete pseudonocardia autotrophica and consisting of 403 amino-acid residues. vdh catalyzes the activation of vitamin d(3) via sequential hydroxylation reactions: these reactions involve the conversion of vitamin d(3) (cholecalciferol or vd3) to 25-hydroxyvitamin d(3) [25(oh)vd3] and the subsequent conversion of 25(oh)vd3 to 1alpha,25-dihydroxyvitamin d(3) [calciferol or 1alpha,25(oh)(2)vd3]. overexpr ... | 2009 | 19342783 |
| molecular characterization of mycobacterium bovis strains isolated from cattle slaughtered at two abattoirs in algeria. | bovine tuberculosis is prevalent in algeria despite governmental attempts to control the disease. the objective of this study was to conduct, for the first time, molecular characterization of a population sample of mycobacterium bovis strains isolated from slaughter cattle in algeria. between august and november 2007, 7250 animals were consecutively screened at the abattoirs of algiers and blida. in 260 animals, gross visible granulomatous lesions were detected and put into culture. bacterial is ... | 2009 | 19173726 |
| biodegradation of thiodiglycol, a hydrolyzate of the chemical weapon yperite, by benzothiophene-desulfurizing bacteria. | microbial degradation of thiodiglycol (bis(2-hydroxyethyl)sulfide, tdg) with petroleum-desulfurizing soil bacteria was examined. among the bacteria tested, several strains belonging to the genera rhodococcus and gordonia grew on tdg as the sole sulfur source. the selected strain rhodococcus sp. strain t09, which was re-identified as r. jostii, showed tdg degradation activity only when grown in the presence of tdg as the sole sulfur source. repeat batch degradation of tdg by using strain t09 coul ... | 2009 | 19179003 |
| biodegradation kinetics of picric acid by rhodococcus sp.njust16 in batch reactors. | biological degradation of 2,4,6-trinitrophenol (tnp) by rhodococcus sp.njust16 in mineral salt medium was investigated in shake-flask experiments at ph of 7.0 and 30 degrees c, over a wide range of initial tnp concentration (20-800 mgl(-1)). the tnp was observed to be the inhibitory compound. for the studied concentration range, haldane's model could be fitted to the growth kinetics data well with the kinetic constants mu(max)=0.2362 h(-1), k(s)=9.9131 mgl(-1) and k(i)=362.7411 mgl(-1). further, ... | 2009 | 19185996 |
| self-subunit swapping chaperone needed for the maturation of multimeric metalloenzyme nitrile hydratase by a subunit exchange mechanism also carries out the oxidation of the metal ligand cysteine residues and insertion of cobalt. | the incorporation of cobalt into low molecular mass nitrile hydratase (l-nhase) of rhodococcus rhodochrous j1 has been found to depend on the alpha-subunit exchange between cobalt-free l-nhase (apo-l-nhase lacking oxidized cysteine residues) and its cobalt-containing mediator (holo-nhlae containing cys-so(2)(-) and cys-so(-) metal ligands), this novel mode of post-translational maturation having been named self-subunit swapping, and nhle having been recognized as a self-subunit swapping chaperon ... | 2009 | 19346246 |
| analysis of benzene-induced effects on rhodococcus sp. 33 reveals that constitutive processes play a major role in conferring tolerance. | most studies investigating mechanisms that confer microorganisms with tolerance to solvents have often focused on adaptive responses following exposure, while less attention has been given to inherent, or constitutive, processes that prevail at the onset of exposure to a toxic solvent. in this study, we investigated several properties of the highly solvent-tolerant bacterium rhodococcus sp. 33 that confer it with a tolerance to high concentrations of benzene. when challenged with liquid benzene, ... | 2009 | 19347232 |
| young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity. | newborn foals are very susceptible to infections by opportunistic pathogens such as rhodococcus equi. this susceptibility is thought to be due to the immaturity of their immune system, in particular their inability to produce interferon-gamma. this deficiency may result from an insufficiency in accessory signals. we therefore compared monocyte-derived dendritic cells (modc) from foals and from adult horses. cd172, mhc-i and mhc-ii were generally expressed on more than 90% modc from foals and adu ... | 2009 | 19349079 |
| the genus dietzia: a new home for some known and emerging opportunist pathogens. | the genus dietzia has only been established fairly recently. the gram morphology and colony appearance of the species of this genus is remarkably similar to rhodococcus equi. in the absence of simple, accurate methods for their identification, dietzia spp. might have been misidentified as a rhodococcus spp. and/or considered to be contaminants only. this minireview is designed to summarize current evidence on the clinical significance of dietzia species, to consider their potential role as human ... | 2009 | 19159434 |
| ventriculo-peritoneal shunt infection due to non-equi rhodococcus sp. | 2009 | 19084452 |