Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| polymorphism in repeated 16s rrna genes is a common property of type strains and environmental isolates of the genus vibrio. | analysis of the 16s rdnas obtained from cultures of single colonies of either type collection strains or environmental strains of the genus vibrio revealed the presence of polymorphism in every one of the strains examined. polymorphism was detected by visualization of heteroduplexes produced after 16s rdna pcr amplification, a procedure that allows for the screening of a large number of isolates. amplified 16s rdnas obtained from both vibrio parahaemolyticus and an environmental strain were clon ... | 2002 | 11932467 |
| pcr and molecular detection for differentiating vibrio species. | vibriosis is an economically important disease of fish, marine invertebrates (particularly penaeid shrimps), and large marine mammals and is responsible for high mortality rates in aquaculture worldwide. some vibrio species are also responsible for zoonoses, whereas others are relatively nonpathogenic. using 16s- and 23s-based pcr reactions, we obtained species-specific patterns and a 470-bp band, respectively. dna sequences obtained on the 23s rrna gene allowed us to identify species-specific p ... | 2002 | 12381564 |
| functional characterization of brucella melitensis normi, an efflux pump belonging to the multidrug and toxic compound extrusion family. | two putative proteins (normi and normii) similar to the multidrug efflux protein norm of vibrio parahaemolyticus are encoded by the brucella melitensis 16 m genome. we show that a drug-hypersusceptible escherichia coli strain overexpressing normi displays increased resistance to norfloxacin, ciprofloxacin, gentamicin, tetraphenylphosphonium ion, acriflavine, and berberine. this elevated resistance was proven to be mediated by an energy-dependent efflux mechanism. normi belongs to the multidrug a ... | 2002 | 12183269 |
| environmental investigation of potentially pathogenic vibrio parahaemolyticus in the seto-inland sea, japan. | seawater and organic material (live and/or dead matter deposited on any substratum submersed in seawater) were collected during the cool weather season from a coast of the seto-inland sea, japan, and analyzed to determine vibrio parahaemolyticus densities and the occurrence of pathogenic strains, defined as those possessing tdh and/or trh genes by the polymerase chain reaction (pcr), using isolated dna from enrichment culture of the samples. about 95% of the samples were positive for v. parahaem ... | 2002 | 11934498 |
| vibrio parahaemolyticus scrabc, a novel operon affecting swarming and capsular polysaccharide regulation. | swarming is an adaptation of many bacteria to growth on surfaces. a search for genes controlling swarmer cell differentiation of vibrio parahaemolyticus identified a novel three-gene operon that potentially encodes a pyridoxal-phosphate-dependent enzyme, an extracellular solute-binding protein, and a membrane-bound ggdef- and eal-motif sensory protein. the functions of these motifs, which are named after conserved amino acid sequences, are unknown, although the domains are found singly and in co ... | 2002 | 12374828 |
| alkaline adaptation induces cross-protection against some environmental stresses and morphological change in vibrio parahaemolyticus. | the relationship between alkaline adaptation and the resistance against environmental stresses was examined in vibrio parahaemolyticus. alkali-adapted cells were found to have increased resistance against various stresses, including heat, crystal violet, deoxycholic acid, and hydrogen peroxide. however, alkali-adapted cells showed no increased resistance against acid stress and heat-adapted cells did not show increased resistance against alkaline stress. furthermore, alkaline treatment induced c ... | 2002 | 12501988 |
| clinical, epidemiological, and socioeconomic analysis of an outbreak of vibrio parahaemolyticus in khanh hoa province, vietnam. | from 1996 onward, a pandemic spread of vibrio parahaemolyticus infections due to one clone has been reported in several asian countries. during a population-based study that relied on passive surveillance, 548 cases of v. parahaemolyticus infection were detected between 1997 and 1999 in the khanh hoa province of vietnam. detection of cases of v. parahaemolyticus infection abruptly stopped in november 1999, although vibrio species other than v. parahaemolyticus continued to be isolated throughout ... | 2002 | 12447738 |
| bactericidal activity of phlorotannins from the brown alga ecklonia kurome. | the bactericidal activity of phlorotannins from brown algae against food-borne pathogenic bacteria (25 strains), methicillin-resistant staphylococcus aureus (mrsa) (nine strains) and streptococcus pyogenes (one strain) was examined and compared with that of catechins. in addition, the effect of the oral administration of phlorotannins on mice was investigated. phlorotannins, which are oligomers of phloroglucinol, were extracted from thalli of the brown alga ecklonia kurome and prepared by silici ... | 2002 | 12461009 |
| characterization by pcr of vibrio parahaemolyticus isolates collected during the 1997-1998 chilean outbreak. | between november 1997 and april 1998, several human gastroenteritis cases were reported in antofagasta, a city in the north of chile. this outbreak was associated with the consumption of shellfish, and the etiologic agent responsible was identified as vibrio parahaemolyticus. this was the first report of this bacterium causing an epidemic in chile. v. parahaemolyticus was the only pathogenic bacterium isolated from patient stools and from shellfish samples. these isolates were analyzed by polyme ... | 2002 | 12462995 |
| automated postprocessing of electrospray lc/ms data for profiling protein expression in bacteria. | we describe an integrated approach for automating protein analysis of bacterial cell extracts. the method uses electrospray lc/ms to generate chromatographic profiles of proteins present in an extract, along with a software program that automates the data analysis. the software program, retana, automates the sequential summing, centroiding, and deconvolution of multiply charged proteins present in consecutive scans of the lc/ms analysis. this procedure generates a concise, single spectrum of pro ... | 2002 | 12463365 |
| phylogenetic study and identification of human pathogenic vibrio species based on partial hsp60 gene sequences. | the use of hsp60 gene sequences for phylogenetic study and identification of pathogenic marine vibrios was investigated. a 600-bp partial hsp60 gene was amplified by pcr and sequenced from 29 strains representing 15 vibrio species within the family vibrionaceae. sequence comparison of the amplified partial hsp60 gene revealed 71-82% sequence identity among different vibrio species and 96-100% sequence identity among epidemiologically distinct strains with the same species designation. this degre ... | 2002 | 12489780 |
| density of total and pathogenic (tdh+) vibrio parahaemolyticus in atlantic and gulf coast molluscan shellfish at harvest. | the densities of total and pathogenic vibrio parahaemolyticus in 671 samples of molluscan shellfish harvested in 1999 and 2000 from 14 sites in seven gulf and atlantic coast states were determined at 2-week intervals over a period of 12 to 16 months in each state. changes in v. parahaemolyticus densities in shellfish between harvest and sample analysis were minimized with time and temperature controls. densities were measured by direct plating techniques, and gene probes were used for identifica ... | 2002 | 12495004 |
| application of a microchamber array for dna amplification using a novel dispensing method. | we recently developed a microchamber array chip for dna amplification by adopting semiconductor microfabrication technology; a polymerase chain reaction (pcr) was performed in the microchamber array, and the amplified dna was detected using a fluorescent dye. in order to manipulate a single cell or sample into each microchamber individually in this system, the chip was directly sealed with a cover glass slip which impeded the retrieval of the products from each chamber. the present study was the ... | 2002 | 12680464 |
| potentiality of a new compound for in vitro differentiation between halophilic and non-halophilic vibrios. | sensitivity of 21 halophilic vibrios and 16 clinical isolates of non-halophilic vibrios was determined against a new possible antivibrio agent, a pyrimidine analogue, 4, 6-dimethylpyrimidine -2-thiol (4,6-dmpt). it appeared to be a vibriocidal agent, having a mean mic and mbc of 32 microg/ml for halophilic strains and 64 microg/ml for non-halophilic strains and an ld50 of 300 mg/kg body weight of mice. thus, 4,6-dmpt may help an in vitro distinction between halophilic and non-halophilic vibrios. ... | 2002 | 12622188 |
| dominant-negative rho, rac, and cdc42 facilitate the invasion process of vibrio parahaemolyticus into caco-2 cells. | to clarify the invasive process of vibrio parahaemolyticus, an invasion assay was performed using cells expressing dominant negative small gtpases of the rho family. this assay showed that the dominant negative host phenotype facilitates bacterial invasion, suggesting that the mechanism of v. parahaemolyticus invasion differs from that reported for other invasive bacteria. | 2002 | 11796633 |
| prevalence of the pandemic genotype of vibrio parahaemolyticus in dhaka, bangladesh, and significance of its distribution across different serotypes. | sixty-six strains of vibrio parahaemolyticus belonging to 14 serotypes were isolated from hospitalized patients in dhaka, bangladesh, from january 1998 to december 2000. among these, 48 strains belonging to four serotypes had the pandemic genotype and possessed the tdh gene. a marker (open reading frame orf8) for a filamentous phage previously thought to correspond to the pandemic genotype was found to have a poor correlation with the pandemic genotype. | 2002 | 11773134 |
| vmra, a member of a novel class of na(+)-coupled multidrug efflux pumps from vibrio parahaemolyticus. | gene vmra, cloned from vibrio parahaemolyticus, made escherichia coli resistant to 4',6-diamino-2-phenylindol, tetraphenylphosphonium chloride, acriflavine, and ethidium bromide. vmra belongs to the dinf branch of mate family efflux transporters. vmra catalyzed acriflavine efflux and showed na(+)/drug transporter activity because the addition of tetraphenylphosphonium to na(+)-loaded cells caused na(+) efflux. | 2002 | 11751837 |
| an investigation into the changed physiological state of vibrio bacteria as a survival mechanism in response to cold temperatures and studies on their sensitivity to heating and freezing. | to induce pathogenic vibrio bacteria into a changed physiological state, in response to cold temperatures in sea water, and assess their sensitivity to heating and freezing, as compared with normal cells. | 2002 | 12010547 |
| occurrence of pathogenic vibrios in coastal areas of france. | this study was carried out to investigate the occurrence of potentially pathogenic species of vibrio in french marine and estuarine environments. | 2002 | 12010553 |
| phytochemical and antimicrobial studies of begonia malabarica. | phytochemical investigation of the various extracts of the leaves of begonia malabarica lam. (begoniaceae) resulted in the isolation and identification of six known compounds, viz. friedelin, epi-friedelinol, beta-sitosterol, luteolin, quercetin and beta-sitosterol-3-beta-d-glucopyranoside. the aqueous and organic solvent extracts were also tested against ten human pathogenic bacteria and four fungal strains by the agar-well diffusion method. all the extracts were devoid of antifungal activity a ... | 2002 | 11744306 |
| dermatofibrosarcoma protuberans--case reports and review of literature. | 2002 | 139685 | |
| [reflection of hydroelectrolytic replacement prior to parenteral feeding]. | 2002 | 120569 | |
| [endogenous factors in acquired cochlear deafness in adults]. | 2002 | 123939 | |
| [histochemical studies on the angle of the anterior chamber in glaucoma. part i. primary open-angle glaucoma (author's transl)]. | 2002 | 130793 | |
| multiple forms of phosphofructokinase in animals from the phylum chordata. | 2002 | 134863 | |
| [general characteristics of the incidence of urolithiasis in chuvash assr]. | 2002 | 135390 | |
| [protective and reparative effect of deoxyribonucleic acid instilled in the cornea]. | 2002 | 135294 | |
| [congenital entropion of the eyelids (2 clinical cases)]. | 2002 | 135290 | |
| long-term treatment with beta-blockers after myocardial infarction. | 162 patients discharged from hospital after mycardial infarction were randomly allocated to two groups, one received alprenolol 400 mg daily and the other served as the control. the period of follow-up was two years and all other treatment given was standardized. the two groups did not differ with respect to risk factors for myocardial infarction, the course of the acute infarct or treatment during follow-up. after two years one patient in the group treated with alprenolol had died suddenly as c ... | 2002 | 134897 |
| removal of an extrauterine iud by combined laparoscopy and colpotomy. | 2002 | 139572 | |
| [lesion of the deep branch of the ulnar nerve caused by fracture of the hook of the hamate]. | we present a case of lesion of the deep branch of ulnar nerve at the wrist caused by fracture of the hook of the hamate. according to wu's classification (1985) based on clinical and electromyographic findings and the clinicoanatomic correlations, our case corresponds to type iv of this classification with a pure motor ulnar neuropathy with sparing of hypothenar muscles. improvement was observed after surgery. | 2002 | 2290409 |
| differences in length of hospital stay for medicaid and blue cross patients and the effect of intensity of services. | 2002 | 113844 | |
| [guideline for land (provincial) laws on public health and their incorporation into current land legislation (author's transl)]. | 2002 | 150564 | |
| [responsiveness of lymphocytes to stimulation by pha and autologous blasts in acute lymphatic leukemias during remission (author's transl)]. | the paper deals with the question how far the lymphocytes of acute lymphatic leukemia patients in remission can be stimulated by pha and autologous blasts in the mlc-test. the lymphocyte stimulation by pha showed considerable variation dependent on the phase of therapy. a significant reduction of the responsiveness to pha stimulation was found during the prophylactic reinduction treatment with vincristin and prednisolon compared with the results during maintenance therapy. the examination of lym ... | 2002 | 135240 |
| [postoperative esophageal stenosis (role of the laryngologist)]. | 2002 | 153373 | |
| clinical comparison between glibornuride (glutril) and tolbutamide in maturity-onset diabetes: a controlled double-blind trial. | 2002 | 134878 | |
| [details of a denounced physician (e.n.t.) suspected of bodily injury, and the following expert judgement (author's transl)]. | an otologist was denounced and the patient's case history was confiscated because after tonsillectomy an absecess and a thrombosis of the vena jugularis interna had developed. the procedure and the medical aspect how at first being judged by the lawyer and the public prosecutor are described. on the base of the given medical opinion the court quashed proceedings. because of the growing number of denouncements this casuistic paper ends with four guiding principles to protect against unjustified m ... | 2002 | 135139 |
| rna biosynthesis in the chick embryo during development and its relation to collagen synthesis. | a study of rna synthesis in the legs and wings of chick embryos suggests synthesis of specific rna's as a function of development. an increase in rna at day 10 was followed by an increase in collagen synthesis. that rna had a sedimentation coefficient of 23s and several of the characteristics of mrna. thus it appeared to contain a poly a sequence since it was retained on poly u coated-filters, it was not methylated, and its synthesis was not inhibited by low doses of actinomycin d. that rna was ... | 2002 | 134868 |
| [late diagnosis of a nasal foreign body]. | 2002 | 135308 | |
| [turakulov ialkin khalmatovich (on his 60th birthday)]. | 2002 | 139606 | |
| [laparoscopy in the diagnosis of retroperitoneal non-organ tumors]. | 2002 | 135356 | |
| [desmoid tumor of the abdominal wall in a young child]. | 2002 | 126412 | |
| [public health in the russian federation during the 9th five-year plan]. | 2002 | 135354 | |
| oligoclonal igg and virus antibodies in cerebrospinal fluid in progressive myoclonus epilepsy. | 2002 | 117579 | |
| oligoclonal igg and virus antibodies in cerebrospinal fluid in progressive myoclonus epilepsy. | 2002 | 117579 | |
| [valve of the study of the mechanism of discoveries. apropos of some medical discoveries, including ménière's disease and perforation of the tympanum by astley-cooper]. | 2002 | 123949 | |
| carbamazepine serum levels in children with epilepsy: a micro immuno-assay technique. | in part 1 of this study, 26 children with seizures were given 20 mg/kg/day carbamazepine and five developed side-effects. with this initial dose, equilibrium was reached in the serum within four to six days in 25 of the 26 children. the sudden withdrawal of other anticonvulsants did not usually affect the rise of carbamazepine in serum, unless given in high doses. in part 2 of the study it was shown that satisfactory levels were obtained in the serum of 38 children given carbamazepine either twi ... | 2002 | 118072 |
| a symposium on the chronic pain syndrome in problem low back cases. psychiatric evaluation and feasibility of psychiatric therapy. | 2002 | 127024 | |
| [laparoscopy of adhesions. diagnostic use]. | 2002 | 134429 | |
| a child of 3 years who developed an encephalitic reaction to mmr (mumps, measles, rubella) immunisation at age 15 months. | 2002 | 2373602 | |
| interaction of butylated hydroxytoluene (bht) with phospholipid bilayer membranes: effect on 22na permeability and membrane fluidity. | 2003 | 201255 | |
| urinary excretion of adenosine 3',5'-monophosphate and guanosine 3',5'-monophosphate in normal children and those with cystic fibrosis. | the urinary excretion of adenosine 3',5'-monophosphate (cyclic amp) and guanosine 3',5'-monophosphate (cyclic gmp) was examined in 98 normal children and 46 children with cystic fibrosis between the ages of 9 months and 18 yr. diurnal variations in cyclic amp and cyclic gmp excretion were observed in subjects from either group, and peak levels of cyclic nucleotide excretion were generally observed during the period of 0700 to 2100 h. excretion rates (mumol/day) of cyclic amp and cyclic gmp incre ... | 2003 | 165210 |
| [overview of the general oncology infrastructure. results of an survey]. | 2003 | 262695 | |
| studies of dynein from tetrahymena cilia using agarose polyacrylamide gel electrophoresis. | described in this report is an application of agarose-polyacrylamide gel electrophoresis, which separates protein components of crude dynein fraction (fraction i by gibbons) derived from tetrahymena cilia. by this method, the fraction was separated into three protein components (designated as bands i, ii and iii) on the gel. when the gel was actively stained for dynein atpase, a single band appeared, which coincided with the position of band i. a purified dynein prepared by controlled pore glass ... | 2003 | 156562 |
| [the presence of acervuli in the pineal gland of prematurely born and full-term newborn (author's transl)]. | 2003 | 262724 | |
| the preservation of bacteria and fungi on anhydrous silica gel: an assessment of survival over four years. | 2003 | 165168 | |
| [gastroschisis and omphalocele. treatment and results]. | 2003 | 150087 | |
| [association of an ossicle malformation and a congenital preauricular fistula]. | 2003 | 142810 | |
| [development of asphyxia in hematogenic osteomyelitis of the mandible in a newborn infant]. | 2003 | 283596 | |
| [fate of a lavsan strip used for stenosing the small intestine]. | 2003 | 142861 | |
| an enzyme histochemical study of non-hodgkin's lymphoma and allied disease. | the enzyme activity for alpha-naphthyl-acetate esterase, naphthol-as-acetate esterase, naphthol-as-d-chloroacetate esterase, acid phosphatase, l(+)-tartrate-resistant acid phosphatase, adenosine triphosphatase, and 5'-nucleotidase was examined on the neoplastic cells of giant follicular lymphoblastoma, the so-called reticulum cell sarcoma and sézary syndrome. the neoplastic cells of giant follicular lymphoblastoma showed distinct activity for adenosine triphosphatase and 5'-nucleotidase, and tho ... | 2003 | 145325 |
| sodium transport ouabain binding, and (na+/k+)-atpase activity in down's syndrome platelets. | 2003 | 149949 | |
| [experience of a handicapped person. 4. interview of patients at a special facility]. | 2003 | 142852 | |
| dangers and interactions between drugs used in conjunction with anaesthesia. | 2003 | 262470 | |
| self-assessment removes pressures of examination. | 2003 | 153860 | |
| statistical analysis of epidemiological data from a chromium chemical manufacturing plant. | a statistical analysis was performed on reported bronchogenic carcinoma incidence in a group of chromium chemical manufacturing workers over the period 1929 to 1977. the purpose was to determine if post-1950 process improvements corresponded to favorable trends in morbidity. because the paucity of data on the population at risk prevented meaningful calculations of smr's, a recently developed method called "probability window analysis" was used to evaluate for statistically valid changes in the " ... | 2003 | 430272 |
| blood chemistry and lipid profiles of elite distance runners. | in summary, we conclude that the analysis of the blood profiles of elite runners offers no explanation for their superior fitness of physical ability when compared to the good runners. selected enzymes related to cellular or tissue damage may be elevated in distance runners and could be classified as abnormal on routine clinical evaluation if unaware of their physical lifestyles. it is also important to note that certain blood profile parameters, especially the hematocrit, could be classified as ... | 2003 | 201207 |
| [a case report of laryngeal tuberculosis with pseudotumoral appearance]. | 2003 | 142806 | |
| a new look at cavity preparation. | 2003 | 298770 | |
| subcrestal retention of endodontically treated roots: discussion and report of case. | 2003 | 296256 | |
| current trends and prospects in surgical adjuvant trials. | new concepts and treatments currently available for adjuvant studies are illustrated by a review of ongoing studies sponsored by the national cancer institute. more thorough information is needed on immunotherapeutic agents to allow more rationale in the use of these agents. solid bases to properly select drugs or drug combinations for adjuvant purposes are being established. however, dose-schedule and duration of treatment are still to be defined. strategies directed at prolonging the benefit o ... | 2003 | 282827 |
| [surgical treatment of cancer of the lung]. | 2003 | 219397 | |
| [dynamic characteristics of the physical development of preschool children]. | 2003 | 152462 | |
| [effectiveness of the comprehensive treatment by rhonidase electrophoresis and tbilisi sulfide baths of elderly patients suffering from osteoarthrosis deformans]. | 2003 | 154769 | |
| [vectorcardiographic aspects of complete right bundle branch block following ventriculotomy (author's transl)]. | 2003 | 148862 | |
| [study of left ventricular function with quantitative angiocardiographic methods]. | 2003 | 161533 | |
| huntington's disease--growth of fibroblast cultures in lipid-deficient medium: a preliminary report. | when skin fibroblast cultures derived from subjects with huntington's disease (hd) were grown in medium supplemented with lipid-deficient serum (lds), growth rates were less than those of control cultures. the mean percentage increase in generation times for nine control cultures incurred by using lds rather than fetal calf serum (fcs) was 8.0%. four cultures from 2 patients with hd failed to grow in lds but grew well in medium supplemented with fcs. in nine other cultures derived from 7 patient ... | 2003 | 152597 |
| [minor burns]. | 2003 | 254377 | |
| a scanning electron microscope study of one year in vivo amalgam-enamel interfaces. | 2003 | 298764 | |
| the bulletin turns forty. | 2003 | 150055 | |
| intercalation activating fluorescence dna probe and its application to homogeneous quantification of a target sequence by isothermal sequence amplification in a closed vessel. | we developed a completely homogeneous and isothermal method of detecting rna sequences and demonstrated ultrarapid and direct quantification of pathogenic gene expression with high sensitivity. the assay is based on performing isothermal rna sequence amplification in the presence of our novel dna probe, an intercalation activating fluorescence dna probe, and measuring the fluorescence intensity of the reaction mixture. when detecting meca gene expression of methicillin-resistant staphylococcus a ... | 2003 | 12633605 |
| detection of pathogenic vibrio parahaemolyticus in oyster enrichments by real time pcr. | a real time polymerase chain reaction (pcr) assay was developed and evaluated to detect the presence of the thermostable direct hemolysin gene (tdh), a current marker of pathogenicity in vibrio parahaemolyticus. the real time pcr fluorogenic probe and primer set was tested against a panel of numerous strains from 13 different bacterial species. only v. parahaemolyticus strains possessing the tdh gene generated a fluorescent signal, and no cross-reaction was observed with tdh negative vibrio or n ... | 2003 | 12654486 |
| inactivation of bacteria in seawater by low-amperage electric current. | seawater used in mariculture has been suspected of being a potential source of infection. in this study, the lethal effects of low-amperage electric treatment on microorganisms were examined in natural seawater and in seawater inoculated with vibrio parahaemolyticus. in both cases, bacteria including v. parahaemolyticus in seawater were completely eliminated in 100 ms by a 0.5-a, 12-v direct current. electron microscopic investigation of the electrically treated bacteria revealed substantial str ... | 2003 | 12676730 |
| representation, dispersion, and variation of bacterial indicators in the coastal waters of nagore (east coast of india). | the occurrence of bacterial indicators in the coastal waters of nagore on the east coast of india was studied during two monsoon and nonmonsoon cycles from 1995 to1996. proteus/klebsiella, vibrio parahaemolyticus, and total coliforms were typically present at a mean level of 10(9) colony-forming units/g dry sediment, whereas other indicators such as salmonella, shigella, and v. cholerae were at the 10(5) level. in the water column, total coliforms and salmonella were 2 to 3 orders of magnitude l ... | 2003 | 12683465 |
| cholera and other types of vibriosis: a story of human pandemics and oysters on the half shell. | vibrios are ubiquitous in the aquatic environment and are commonly present in or on shellfish and other seafood. a small subset of strains/species are able to cause human disease, including the cholera toxin-producing strains of vibrio cholerae that are responsible for epidemic/pandemic cholera; thermostable direct hemolysin-producing strains of vibrio parahaemolyticus; and vibrio vulnificus, which can cause fulminant sepsis. cholera outbreaks can be initiated by transmission of "epidemic" v. ch ... | 2003 | 12856219 |
| structure and serological characterization of 5,7-diamino-3,5,7,9-tetradeoxy-non-2-ulosonic acid isolated from lipopolysaccharides of vibrio parahaemolyticus o2 and o-untypable strain kx-v212. | lipopolysaccharides (lps) of vibrio parahaemolyticus o2 and o-untypable (out) strain (kx-v212) isolated from an individual patient were shown to contain 5,7-diamino-3,5,7,9-tetradeoxy-non-2-ulosonic acid (nonla), which was readily released from lps by mild acid hydrolysis. in the present study, we investigated the chemical and serological properties of nonla isolated from lps of v. parahaemolyticus o2 and out kx-v212. gc-ms and nmr analysis identified the nonla from lps of o2 to be 5,7-diacetami ... | 2003 | 12706971 |
| structural analysis of the carbohydrate backbone of vibrio parahaemolyticus o2 lipopolysaccharides. | a structural investigation has been carried out on the carbohydrate backbone of vibrio parahaemolyticus o2 lipopolysaccharides (lps) isolated by dephosphorylation, o-deacylation and n-deacylation. the carbohydrate backbone is a short-chain saccharide consisting of nine monosaccharide units i.e., 1 mol each of d-galactose (gal), d-glucose (glc), d-glucuronic acid (glca), l-glycero-d-manno-heptose (l,d-hep), d-glycero-d-manno-heptose (d,d-hep), 3-deoxy-d-manno-oct-2-ulosonic acid (kdo), 5,7-diacet ... | 2003 | 12706972 |
| an iron-regulated gene required for utilization of aerobactin as an exogenous siderophore in vibrio parahaemolyticus. | a previous investigation using the fur titration assay system showed that vibrio parahaemolyticus possesses a gene encoding a protein homologous to iuta, the outer-membrane receptor for ferric aerobactin in escherichia coli. in this study, a 5.6 kb dna region from the v. parahaemolyticus wp1 genome was cloned and two entire genes, iuta and alcd homologues, were identified which are absent from vibrio cholerae genomic sequences. the v. parahaemolyticus iuta and alcd proteins share 43 % identity w ... | 2003 | 12724383 |
| molecular, serological, and virulence characteristics of vibrio parahaemolyticus isolated from environmental, food, and clinical sources in north america and asia. | potential virulence attributes, serotypes, and ribotypes were determined for 178 pathogenic vibrio parahaemolyticus isolates from clinical, environmental, and food sources on the pacific, atlantic, and gulf coasts of the united states and from clinical sources in asia. the food and environmental isolates were generally from oysters, and they were defined as being pathogenic by using dna probes to detect the presence of the thermostable direct hemolysin (tdh) gene. the clinical isolates from the ... | 2003 | 12839774 |
| studies on pathogenic vibrio parahaemolyticus during a warm weather season in the seto inland sea, japan. | vibrio parahaemolyticus is a potentially pathogenic bacterium, occurring naturally in estuarine and marine environments throughout the world. the incidence of this organism in an aquatic environment depends upon many ecofactors. sea water and organic material were collected during the warm weather season from a coast of the seto inland sea, japan, and analysed to determine v. parahaemolyticus densities and the occurrence of pathogenic strains, defined as those possessing tdh and/or trh genes by ... | 2003 | 12871237 |
| flagellar basal body flg operon as a virulence determinant of vibrio vulnificus. | vibrio vulnificus, a halophilic estuarine bacterium causing a rapidly progressing fatal septicemia, is highly cytotoxic to eukaryotic cells. to identify new virulence factors associated with cytotoxicity, we constructed a mariner-based transposon (tn himar1) library of the highly virulent clinical isolate mo6-24/o having a double mutation in the hemolysin and protease genes. the himar1 mutant library was extensively screened for the mutants showing decreased cytotoxicity to hela cells. we select ... | 2003 | 12711330 |
| the urease gene cluster of vibrio parahaemolyticus does not influence the expression of the thermostable direct hemolysin (tdh) gene or the tdh-related hemolysin gene. | in order to investigate why the thermostable direct hemolysin (tdh) and the tdh-related hemolysin (trh) of vibrio parahaemolyticus are produced at low levels from urease-positive strains, the effect of the functional urease gene cluster of v. parahaemolyticus on the expression of the tdh and trh genes was examined. transcriptional lacz fusions with the tdh1, tdh2, trh1 and trh2 genes representing variants of the tdh and trh genes were integrated into the chromosome of an escherichia coli strain ... | 2003 | 12725294 |
| amlodipine: a cardiovascular drug with powerful antimicrobial property. | ten cardiovascular drugs were procured in pure form from their manufacturers in india and screened for antimicrobial property against fifteen known bacteria belonging to both gram-positive and gram-negative types. these bacteria were inhibited by the common antibiotics at 1-5 mg ml(-1) level through our earlier studies. since most of the bacteria were moderate to highly responsive to amlodipine, this compound was further tested in vitro against 504 bacteria comprising 4 genera of gram-positive a ... | 2003 | 14743981 |
| accuracy of six commercially available systems for identification of members of the family vibrionaceae. | six commercially available bacterial identification products were tested with vibrio alginolyticus (12 strains), v. cholerae (30 strains), photobacterium (vibrio) damselae (10 strains), v. fluvialis (10 strains), v. furnissii (4 strains), v. hollisae (10 strains), v. metschnikovii (9 strains), v. mimicus (10 strains), v. parahaemolyticus (30 strains), and v. vulnificus (10 strains) to determine the accuracy of each system for identification. the products included api 20e, crystal e/nf, microscan ... | 2003 | 14662957 |
| antimicrobial performance of alkaline ionic fluid (gc-100x) and its ability to remove escherichia coli o157:h7 from the surface of tomatoes. | an efficacy test of gc-100x, a noncorrosive alkaline ionic fluid (ph 12) composed of free radicals and supplemented with xylitol, was carried out against six major foodborne pathogens-staphylococcus aureus fri 913, salmonella enterica serovar enteritidis atcc 13076, s. enterica serovar typhimurium dt104 korean isolate, vibrio parahaemolyticus atcc 17803, escherichia coli o157:h7 atcc 43894, and pseudomonas aeruginosa kctc 1637-at three different temperatures (4, 25, and 36 degrees c) with or wit ... | 2003 | 14503713 |
| increased sensitivity in pcr detection of tdh-positive vibrio parahaemolyticus in seafood with purified template dna. | pcr is an important method for the detection of thermostable direct hemolysin gene (tdh)-positive (pathogenic hemolysin-producing) strains of vibrio parahaemolyticus in seafood because tdh-negative (nonpathogenic) v. parahaemolyticus strains often contaminate seafood and interfere with the direct isolation of tdh-positive v. parahaemolyticus. in this study, the use of pcr to detect the tdh gene of v. parahaemolyticus in various seafoods artificially contaminated with tdh-positive v. parahaemolyt ... | 2003 | 14503724 |
| [rapid detection and identification of enteropathogenic bacteria by a single strain counting-pcr assay]. | the arbitrarily primed-pcr (ap-pcr) method is a technique to generate patterns of randomly amplified dna fragments, and is often used for typing clinical isolates. currently, we have been developing a modified ap-pcr method, the single strain counting-pcr (ssc-pcr), in order to discriminate among bacterial strains based on amplified patterns with multiple primers. here, we describe the availabilities of the ssc-pcr method to identify and detect enteropathogenic bacteria. the analysis of the ampl ... | 2003 | 14509944 |
| purification and characterization of an antibacterial protein in the skin secretion of rockfish sebastes schlegeli. | an antibacterial protein in the skin secretion of rockfish (sebastes schlegeli) was purified by lectin affinity chromatography on con a-sepharose and gel filtration on tskgel g3000sw. the antibacterial protein featured the high molecular mass and selective action against gram-negative bacteria. the molecular mass of the protein was estimated to be approximately 150 kda in gel filtration and approximately 75 kda by sds-page, suggesting that it is dimeric. the antibacterial principle was an acidic ... | 2003 | 14522599 |
| genotypic analyses of vibrio parahaemolyticus and development of a pandemic group-specific multiplex pcr assay. | a total of 54 vibrio parahaemolyticus strains including pandemic o3:k6 strains and newly emerged o4:k68, o1:k25, o1:k26, and o1:k untypeable strains (collectively referred to as the "pandemic group") were examined for their pulsed-field gel electrophoresis (pfge) and arbitrarily primed pcr (ap-pcr) profiles and for the presence or absence of genetic marker dna sequences, toxrs/new or orf8, that had been reported elsewhere to be specific for the pandemic group. both pfge and ap-pcr analyses indic ... | 2003 | 14532202 |
| identification of tdh-positive vibrio parahaemolyticus from an outbreak associated with raw oyster consumption in spain. | between august and september 1999, a total of 64 cases of illness were identified in three episodes of acute gastroenteritis associated with the consumption of live oysters from a typical outdoor street market in galicia (northwest spain). nine case patients were hospitalized and analysis of their stool samples revealed the presence of vibrio parahaemolyticus. the strains isolated from two stool samples were studied for antibiotic susceptibility, biochemical characteristics and presence of virul ... | 2003 | 14553923 |