Publications

alkaline adaptation induces cross-protection against some environmental stresses and morphological change in vibrio parahaemolyticus.	the relationship between alkaline adaptation and the resistance against environmental stresses was examined in vibrio parahaemolyticus. alkali-adapted cells were found to have increased resistance against various stresses, including heat, crystal violet, deoxycholic acid, and hydrogen peroxide. however, alkali-adapted cells showed no increased resistance against acid stress and heat-adapted cells did not show increased resistance against alkaline stress. furthermore, alkaline treatment induced c ...	2002	12501988
application of a microchamber array for dna amplification using a novel dispensing method.	we recently developed a microchamber array chip for dna amplification by adopting semiconductor microfabrication technology; a polymerase chain reaction (pcr) was performed in the microchamber array, and the amplified dna was detected using a fluorescent dye. in order to manipulate a single cell or sample into each microchamber individually in this system, the chip was directly sealed with a cover glass slip which impeded the retrieval of the products from each chamber. the present study was the ...	2002	12680464
a ligand-dependent conformational change of the na+/galactose cotransporter of vibrio parahaemolyticus, monitored by tryptophan fluorescence.	purification and reconstitution of the active vibrio parahaemolyticus na+/galactose transporter (vsglt) enables us to do protein chemistry studies on a representative member of this class of membrane transporters. by measuring intrinsic tryptophan (trp) fluorescence, conformational changes on the binding of substrates could be investigated. trp fluorescence increased by 6% on the addition of saturating levels of both na+ and d-galactose, with a k0.5 for d-galactose of 0.6 mm. no change was seen ...	2002	11891582
effects of heat shock on the thermotolerance, protein composition, and toxin production of vibrio parahaemolyticus.	vibrio parahaemolyticus, an important seafood-associated enteropathogen, usually encounters different adverse conditions in its native or food-processing environment, and the stresses resulting from these conditions may affect the survival of this pathogen and thus change its risk with regard to food hygiene. in this study, we investigated the thermotolerance of v. parahaemolyticus under sublethal heat shock and characterized this response by examining the changes in protein profiles and toxin p ...	2002	11899049
antimicrobial synergistic effect of linolenic acid and monoglyceride against bacillus cereus and staphylococcus aureus.	the antimicrobial effect of linolenic acid with or without monoglyceride (glycerol laurate or glycerol myristate) against six food-borne microorganisms was determined in broth medium. minimum inhibitory concentration of linolenic acid on bacillus cereus and staphylococcus aureus was 20 and 50 ppm, respectively. the growth of b. cereus treated with linolenic acid at 10 ppm with 10 ppm monoglyceride was more inhibitory than that of linolenic acid alone, and the viable cell population was reduced 2 ...	2002	11902977
levels of thermostable direct hemolysin produced by vibrio parahaemolyticus o3:k6 and other serovars grown anaerobically with the presence of a bile acid.	twenty-three v. parahaemolyticus strains, including 12 pandemic o3:k6 strains, were examined for their growth and production of thermostable direct hemolysin (tdh) under an anaerobic culture condition with or without presence of a bile acid, taurocholic acid (tca). both bacterial growth and tdh production were markedly enhanced by tca for a majority of the strains, but the scale of the tdh production was disproportionately greater than that of the corresponding growth for 14 strains. such enhanc ...	2002	11910503
antibacterial activity of chitosans and chitosan oligomers with different molecular weights.	antibacterial activities of six chitosans and six chitosan oligomers with different molecular weights (mws) were examined against four gram-negative (escherichia coli, pseudomonas fluorescens, salmonella typhimurium, and vibrio parahaemolyticus) and seven gram-positive bacteria (listeria monocytogenes, bacillus megaterium, b. cereus, staphylococcus aureus, lactobacillus plantarum, l. brevis, and l. bulgaricus). chitosans showed higher antibacterial activities than chitosan oligomers and markedly ...	2002	11929171
polymorphism in repeated 16s rrna genes is a common property of type strains and environmental isolates of the genus vibrio.	analysis of the 16s rdnas obtained from cultures of single colonies of either type collection strains or environmental strains of the genus vibrio revealed the presence of polymorphism in every one of the strains examined. polymorphism was detected by visualization of heteroduplexes produced after 16s rdna pcr amplification, a procedure that allows for the screening of a large number of isolates. amplified 16s rdnas obtained from both vibrio parahaemolyticus and an environmental strain were clon ...	2002	11932467
environmental investigation of potentially pathogenic vibrio parahaemolyticus in the seto-inland sea, japan.	seawater and organic material (live and/or dead matter deposited on any substratum submersed in seawater) were collected during the cool weather season from a coast of the seto-inland sea, japan, and analyzed to determine vibrio parahaemolyticus densities and the occurrence of pathogenic strains, defined as those possessing tdh and/or trh genes by the polymerase chain reaction (pcr), using isolated dna from enrichment culture of the samples. about 95% of the samples were positive for v. parahaem ...	2002	11934498
dominant-negative rho, rac, and cdc42 facilitate the invasion process of vibrio parahaemolyticus into caco-2 cells.	to clarify the invasive process of vibrio parahaemolyticus, an invasion assay was performed using cells expressing dominant negative small gtpases of the rho family. this assay showed that the dominant negative host phenotype facilitates bacterial invasion, suggesting that the mechanism of v. parahaemolyticus invasion differs from that reported for other invasive bacteria.	2002	11796633
identification and characterization of pvua, a gene encoding the ferric vibrioferrin receptor protein in vibrio parahaemolyticus.	we previously reported that vibrio parahaemolyticus expresses two outer membrane proteins of 78 and 83 kda concomitant with production of siderophore vibrioferrin in response to iron starvation stress and that these proteins are the ferric vibrioferrin receptor and heme receptor, respectively (s. yamamoto, t. akiyama, n. okujo, s. matsuura, and s. shinoda, microbiol. immunol. 39:759-766, 1995; s. yamamoto, y. hara, k. tomochika, and s. shinoda, fems microbiol. lett. 128:195-200, 1995). in this s ...	2002	11807053
vibrio vulnificus and vibrio parahaemolyticus in u.s. retail shell oysters: a national survey from june 1998 to july 1999.	from june 1998 to july 1999, 370 lots of oysters in the shell were sampled at 275 different establishments (71%, restaurants or oyster bars; 27%, retail seafood markets: and 2%, wholesale seafood markets) in coastal and inland markets throughout the united states. the oysters were harvested from the gulf (49%). pacific (14%), mid-atlantic (18%), and north atlantic (11%) coasts of the united states and from canada (8%). densities of vibrio vulnificus and vibrio parahaemolyticus were determined us ...	2002	11808810
characterization of united states outbreak isolates of vibrio parahaemolyticus using enterobacterial repetitive intergenic consensus (eric) pcr and development of a rapid pcr method for detection of o3:k6 isolates.	outbreaks of vibrio parahaemolyticus gastroenteritis in the united states (texas, new york and pacific northwest) in 1997-98 emphasized the need to develop molecular methods for identification and differentiation of these organisms. when outbreak isolates were analyzed for the enterobacterial repetitive intergenic consensus sequences, the texas and new york outbreak isolates had a specific 850-bp dna fragment that was absent in pacific northwest isolates. the 850-bp polymerase chain reaction (pc ...	2002	11814665
microbial risks in mild hot smoking of fish.	hot smoked fish should fulfill the requirements set up for ready-to-eat foods. the total bacterial count on the raw material is 10(3) to 10(5) cfu per cm2 of skin and 10(2) to 10(9) cfu per gram of intestines. contamination with pathogenic bacteria is very low, mainly with clostridium botulinum, listeria monocytogenes, staphylococcus aureus, and vibrio parahaemolyticus. in the premises of low hygiene, the contamination may increase due to unsanitary procedures, rotation of assigned duties of wor ...	2002	11833636
cloning and sequence analysis of a novel metalloprotease gene from vibrio parahaemolyticus 04.	the metalloprotease gene (vppc) from vibrio parahaemolyticus 04 has been cloned and sequenced. the vppc gene contains an open reading frame of 2442 nucleotides encoding a polypeptide of 814 amino acids with a calculated molecular mass of 89,833 da. the predicted amino acid sequence of vppc containing a zinc metalloprotease hexxh consensus motif displays extensive homology to the collagenase from vibrio alginolyticus. the activity of the recombinant protease produced in escherichia coli was exami ...	2002	11867235
effects of depuration of molluscs experimentally contaminated with escherichia coli, vibrio cholerae 01 and vibrio parahaemolyticus.	the aim of the present study was to investigate the behaviour of two pathogenic vibrios (vibrio cholerae o1 and vibrio parahaemolyticus) during depuration and to compare it with that of escherichia coli, used as an indicator of suitability for consumption.	2002	11872121
identification and functional characterization of flgm, a gene encoding the anti-sigma 28 factor in pseudomonas aeruginosa.	we describe here the functional characterization of the putative flgm gene of pseudomonas aeruginosa. flgm of p. aeruginosa is most similar to flgm of vibrio parahaemolyticus. a conserved region is present in the c-terminal half of the flgm of p. aeruginosa and in flgm homologues of other organisms that includes the sigma(28) binding domain. a role for the flgm gene of p. aeruginosa in motility was demonstrated by its inactivation. the beta-galactosidase activity of a transcriptional fusion of t ...	2002	11872701
molecular analysis of vibrio parahaemolyticus isolated from human patients and shellfish during us pacific north-west outbreaks.	aims: the objective of this study was to investigate the occurrence and distribution of haemolysin genes, plasmid profile, serogroup analysis and cellular urease activity for vibrio parahaemolyticus isolates from infected human patients and oysters from the pacific north-western united states between 1988 and 1997. methods and results: all of the clinical and environmental isolates tested in this study exhibited the presence of the thermolabile haemolysin gene, tl, confirming that all of the iso ...	2002	11874534
phytochemical and antimicrobial studies of begonia malabarica.	phytochemical investigation of the various extracts of the leaves of begonia malabarica lam. (begoniaceae) resulted in the isolation and identification of six known compounds, viz. friedelin, epi-friedelinol, beta-sitosterol, luteolin, quercetin and beta-sitosterol-3-beta-d-glucopyranoside. the aqueous and organic solvent extracts were also tested against ten human pathogenic bacteria and four fungal strains by the agar-well diffusion method. all the extracts were devoid of antifungal activity a ...	2002	11744306
vmra, a member of a novel class of na(+)-coupled multidrug efflux pumps from vibrio parahaemolyticus.	gene vmra, cloned from vibrio parahaemolyticus, made escherichia coli resistant to 4',6-diamino-2-phenylindol, tetraphenylphosphonium chloride, acriflavine, and ethidium bromide. vmra belongs to the dinf branch of mate family efflux transporters. vmra catalyzed acriflavine efflux and showed na(+)/drug transporter activity because the addition of tetraphenylphosphonium to na(+)-loaded cells caused na(+) efflux.	2002	11751837
[guideline for land (provincial) laws on public health and their incorporation into current land legislation (author's transl)].		2002	150564
[responsiveness of lymphocytes to stimulation by pha and autologous blasts in acute lymphatic leukemias during remission (author's transl)].	the paper deals with the question how far the lymphocytes of acute lymphatic leukemia patients in remission can be stimulated by pha and autologous blasts in the mlc-test. the lymphocyte stimulation by pha showed considerable variation dependent on the phase of therapy. a significant reduction of the responsiveness to pha stimulation was found during the prophylactic reinduction treatment with vincristin and prednisolon compared with the results during maintenance therapy. the examination of lym ...	2002	135240
[postoperative esophageal stenosis (role of the laryngologist)].		2002	153373
dermatofibrosarcoma protuberans--case reports and review of literature.		2002	139685
[reflection of hydroelectrolytic replacement prior to parenteral feeding].		2002	120569
[endogenous factors in acquired cochlear deafness in adults].		2002	123939
[general characteristics of the incidence of urolithiasis in chuvash assr].		2002	135390
[protective and reparative effect of deoxyribonucleic acid instilled in the cornea].		2002	135294
[congenital entropion of the eyelids (2 clinical cases)].		2002	135290
[histochemical studies on the angle of the anterior chamber in glaucoma. part i. primary open-angle glaucoma (author's transl)].		2002	130793
[lesion of the deep branch of the ulnar nerve caused by fracture of the hook of the hamate].	we present a case of lesion of the deep branch of ulnar nerve at the wrist caused by fracture of the hook of the hamate. according to wu's classification (1985) based on clinical and electromyographic findings and the clinicoanatomic correlations, our case corresponds to type iv of this classification with a pure motor ulnar neuropathy with sparing of hypothenar muscles. improvement was observed after surgery.	2002	2290409
differences in length of hospital stay for medicaid and blue cross patients and the effect of intensity of services.		2002	113844
long-term treatment with beta-blockers after myocardial infarction.	162 patients discharged from hospital after mycardial infarction were randomly allocated to two groups, one received alprenolol 400 mg daily and the other served as the control. the period of follow-up was two years and all other treatment given was standardized. the two groups did not differ with respect to risk factors for myocardial infarction, the course of the acute infarct or treatment during follow-up. after two years one patient in the group treated with alprenolol had died suddenly as c ...	2002	134897
multiple forms of phosphofructokinase in animals from the phylum chordata.		2002	134863
removal of an extrauterine iud by combined laparoscopy and colpotomy.		2002	139572
clinical comparison between glibornuride (glutril) and tolbutamide in maturity-onset diabetes: a controlled double-blind trial.		2002	134878
[late diagnosis of a nasal foreign body].		2002	135308
[public health in the russian federation during the 9th five-year plan].		2002	135354
oligoclonal igg and virus antibodies in cerebrospinal fluid in progressive myoclonus epilepsy.		2002	117579
oligoclonal igg and virus antibodies in cerebrospinal fluid in progressive myoclonus epilepsy.		2002	117579
[turakulov ialkin khalmatovich (on his 60th birthday)].		2002	139606
[laparoscopy in the diagnosis of retroperitoneal non-organ tumors].		2002	135356
[desmoid tumor of the abdominal wall in a young child].		2002	126412
[details of a denounced physician (e.n.t.) suspected of bodily injury, and the following expert judgement (author's transl)].	an otologist was denounced and the patient's case history was confiscated because after tonsillectomy an absecess and a thrombosis of the vena jugularis interna had developed. the procedure and the medical aspect how at first being judged by the lawyer and the public prosecutor are described. on the base of the given medical opinion the court quashed proceedings. because of the growing number of denouncements this casuistic paper ends with four guiding principles to protect against unjustified m ...	2002	135139
rna biosynthesis in the chick embryo during development and its relation to collagen synthesis.	a study of rna synthesis in the legs and wings of chick embryos suggests synthesis of specific rna's as a function of development. an increase in rna at day 10 was followed by an increase in collagen synthesis. that rna had a sedimentation coefficient of 23s and several of the characteristics of mrna. thus it appeared to contain a poly a sequence since it was retained on poly u coated-filters, it was not methylated, and its synthesis was not inhibited by low doses of actinomycin d. that rna was ...	2002	134868
[laparoscopy of adhesions. diagnostic use].		2002	134429
a child of 3 years who developed an encephalitic reaction to mmr (mumps, measles, rubella) immunisation at age 15 months.		2002	2373602
[valve of the study of the mechanism of discoveries. apropos of some medical discoveries, including ménière's disease and perforation of the tympanum by astley-cooper].		2002	123949
carbamazepine serum levels in children with epilepsy: a micro immuno-assay technique.	in part 1 of this study, 26 children with seizures were given 20 mg/kg/day carbamazepine and five developed side-effects. with this initial dose, equilibrium was reached in the serum within four to six days in 25 of the 26 children. the sudden withdrawal of other anticonvulsants did not usually affect the rise of carbamazepine in serum, unless given in high doses. in part 2 of the study it was shown that satisfactory levels were obtained in the serum of 38 children given carbamazepine either twi ...	2002	118072
a symposium on the chronic pain syndrome in problem low back cases. psychiatric evaluation and feasibility of psychiatric therapy.		2002	127024
[overview of the general oncology infrastructure. results of an survey].		2003	262695
interaction of butylated hydroxytoluene (bht) with phospholipid bilayer membranes: effect on 22na permeability and membrane fluidity.		2003	201255
urinary excretion of adenosine 3',5'-monophosphate and guanosine 3',5'-monophosphate in normal children and those with cystic fibrosis.	the urinary excretion of adenosine 3',5'-monophosphate (cyclic amp) and guanosine 3',5'-monophosphate (cyclic gmp) was examined in 98 normal children and 46 children with cystic fibrosis between the ages of 9 months and 18 yr. diurnal variations in cyclic amp and cyclic gmp excretion were observed in subjects from either group, and peak levels of cyclic nucleotide excretion were generally observed during the period of 0700 to 2100 h. excretion rates (mumol/day) of cyclic amp and cyclic gmp incre ...	2003	165210
studies of dynein from tetrahymena cilia using agarose polyacrylamide gel electrophoresis.	described in this report is an application of agarose-polyacrylamide gel electrophoresis, which separates protein components of crude dynein fraction (fraction i by gibbons) derived from tetrahymena cilia. by this method, the fraction was separated into three protein components (designated as bands i, ii and iii) on the gel. when the gel was actively stained for dynein atpase, a single band appeared, which coincided with the position of band i. a purified dynein prepared by controlled pore glass ...	2003	156562
[the presence of acervuli in the pineal gland of prematurely born and full-term newborn (author's transl)].		2003	262724
the preservation of bacteria and fungi on anhydrous silica gel: an assessment of survival over four years.		2003	165168
dangers and interactions between drugs used in conjunction with anaesthesia.		2003	262470
self-assessment removes pressures of examination.		2003	153860
[effectiveness of the comprehensive treatment by rhonidase electrophoresis and tbilisi sulfide baths of elderly patients suffering from osteoarthrosis deformans].		2003	154769
current trends and prospects in surgical adjuvant trials.	new concepts and treatments currently available for adjuvant studies are illustrated by a review of ongoing studies sponsored by the national cancer institute. more thorough information is needed on immunotherapeutic agents to allow more rationale in the use of these agents. solid bases to properly select drugs or drug combinations for adjuvant purposes are being established. however, dose-schedule and duration of treatment are still to be defined. strategies directed at prolonging the benefit o ...	2003	282827
[dynamic characteristics of the physical development of preschool children].		2003	152462
[surgical treatment of cancer of the lung].		2003	219397
[vectorcardiographic aspects of complete right bundle branch block following ventriculotomy (author's transl)].		2003	148862
[association of an ossicle malformation and a congenital preauricular fistula].		2003	142810
[development of asphyxia in hematogenic osteomyelitis of the mandible in a newborn infant].		2003	283596
[gastroschisis and omphalocele. treatment and results].		2003	150087
[fate of a lavsan strip used for stenosing the small intestine].		2003	142861
an enzyme histochemical study of non-hodgkin's lymphoma and allied disease.	the enzyme activity for alpha-naphthyl-acetate esterase, naphthol-as-acetate esterase, naphthol-as-d-chloroacetate esterase, acid phosphatase, l(+)-tartrate-resistant acid phosphatase, adenosine triphosphatase, and 5'-nucleotidase was examined on the neoplastic cells of giant follicular lymphoblastoma, the so-called reticulum cell sarcoma and sézary syndrome. the neoplastic cells of giant follicular lymphoblastoma showed distinct activity for adenosine triphosphatase and 5'-nucleotidase, and tho ...	2003	145325
sodium transport ouabain binding, and (na+/k+)-atpase activity in down's syndrome platelets.		2003	149949
[experience of a handicapped person. 4. interview of patients at a special facility].		2003	142852
statistical analysis of epidemiological data from a chromium chemical manufacturing plant.	a statistical analysis was performed on reported bronchogenic carcinoma incidence in a group of chromium chemical manufacturing workers over the period 1929 to 1977. the purpose was to determine if post-1950 process improvements corresponded to favorable trends in morbidity. because the paucity of data on the population at risk prevented meaningful calculations of smr's, a recently developed method called "probability window analysis" was used to evaluate for statistically valid changes in the " ...	2003	430272
[study of left ventricular function with quantitative angiocardiographic methods].		2003	161533
huntington's disease--growth of fibroblast cultures in lipid-deficient medium: a preliminary report.	when skin fibroblast cultures derived from subjects with huntington's disease (hd) were grown in medium supplemented with lipid-deficient serum (lds), growth rates were less than those of control cultures. the mean percentage increase in generation times for nine control cultures incurred by using lds rather than fetal calf serum (fcs) was 8.0%. four cultures from 2 patients with hd failed to grow in lds but grew well in medium supplemented with fcs. in nine other cultures derived from 7 patient ...	2003	152597
[minor burns].		2003	254377
a scanning electron microscope study of one year in vivo amalgam-enamel interfaces.		2003	298764
blood chemistry and lipid profiles of elite distance runners.	in summary, we conclude that the analysis of the blood profiles of elite runners offers no explanation for their superior fitness of physical ability when compared to the good runners. selected enzymes related to cellular or tissue damage may be elevated in distance runners and could be classified as abnormal on routine clinical evaluation if unaware of their physical lifestyles. it is also important to note that certain blood profile parameters, especially the hematocrit, could be classified as ...	2003	201207
subcrestal retention of endodontically treated roots: discussion and report of case.		2003	296256
[a case report of laryngeal tuberculosis with pseudotumoral appearance].		2003	142806
a new look at cavity preparation.		2003	298770
the bulletin turns forty.		2003	150055
representation, dispersion, and variation of bacterial indicators in the coastal waters of nagore (east coast of india).	the occurrence of bacterial indicators in the coastal waters of nagore on the east coast of india was studied during two monsoon and nonmonsoon cycles from 1995 to1996. proteus/klebsiella, vibrio parahaemolyticus, and total coliforms were typically present at a mean level of 10(9) colony-forming units/g dry sediment, whereas other indicators such as salmonella, shigella, and v. cholerae were at the 10(5) level. in the water column, total coliforms and salmonella were 2 to 3 orders of magnitude l ...	2003	12683465
structure and serological characterization of 5,7-diamino-3,5,7,9-tetradeoxy-non-2-ulosonic acid isolated from lipopolysaccharides of vibrio parahaemolyticus o2 and o-untypable strain kx-v212.	lipopolysaccharides (lps) of vibrio parahaemolyticus o2 and o-untypable (out) strain (kx-v212) isolated from an individual patient were shown to contain 5,7-diamino-3,5,7,9-tetradeoxy-non-2-ulosonic acid (nonla), which was readily released from lps by mild acid hydrolysis. in the present study, we investigated the chemical and serological properties of nonla isolated from lps of v. parahaemolyticus o2 and out kx-v212. gc-ms and nmr analysis identified the nonla from lps of o2 to be 5,7-diacetami ...	2003	12706971
structural analysis of the carbohydrate backbone of vibrio parahaemolyticus o2 lipopolysaccharides.	a structural investigation has been carried out on the carbohydrate backbone of vibrio parahaemolyticus o2 lipopolysaccharides (lps) isolated by dephosphorylation, o-deacylation and n-deacylation. the carbohydrate backbone is a short-chain saccharide consisting of nine monosaccharide units i.e., 1 mol each of d-galactose (gal), d-glucose (glc), d-glucuronic acid (glca), l-glycero-d-manno-heptose (l,d-hep), d-glycero-d-manno-heptose (d,d-hep), 3-deoxy-d-manno-oct-2-ulosonic acid (kdo), 5,7-diacet ...	2003	12706972
flagellar basal body flg operon as a virulence determinant of vibrio vulnificus.	vibrio vulnificus, a halophilic estuarine bacterium causing a rapidly progressing fatal septicemia, is highly cytotoxic to eukaryotic cells. to identify new virulence factors associated with cytotoxicity, we constructed a mariner-based transposon (tn himar1) library of the highly virulent clinical isolate mo6-24/o having a double mutation in the hemolysin and protease genes. the himar1 mutant library was extensively screened for the mutants showing decreased cytotoxicity to hela cells. we select ...	2003	12711330
[vibrios (vibrio cholerae, v. parahaemolyticus, v. vulnificus)].		2003	12718054
[noncholera vibrio infections (v. parahaemolyticus, v. vulnificus and others)].		2003	12718068
an iron-regulated gene required for utilization of aerobactin as an exogenous siderophore in vibrio parahaemolyticus.	a previous investigation using the fur titration assay system showed that vibrio parahaemolyticus possesses a gene encoding a protein homologous to iuta, the outer-membrane receptor for ferric aerobactin in escherichia coli. in this study, a 5.6 kb dna region from the v. parahaemolyticus wp1 genome was cloned and two entire genes, iuta and alcd homologues, were identified which are absent from vibrio cholerae genomic sequences. the v. parahaemolyticus iuta and alcd proteins share 43 % identity w ...	2003	12724383
serum antibody levels against select bacterial pathogens in atlantic bottlenose dolphins, tursiops truncatus, from beaufort nc usa and charleston harbor, charleston, sc, usa.	concern over the emergence of zoonotic diseases in marine organisms is growing. in response to this concern, this study set out to measure antibody activities against bacterial pathogens in atlantic bottlenose dolphins, tursiops truncatus, from the coastal estuaries of nc and sc, usa. individuals from charleston sc harbor, a heavily industrialized shipping harbor estuary, and from beaufort nc, a non-shipping estuary, were examined. purified igg was obtained from pooled sera using ammonium sulfat ...	2003	12502036
lysis of methicillin-resistant staphylococcus aureus by 2,4-diacetylphloroglucinol produced by pseudomonas sp. amsn isolated from a marine alga.	previously 2,4-diacetylphloroglucinol (dapg) produced by pseudomonas sp. amsn isolated from a marine alga, had demonstrated a high level of anti-methicillin-resistant staphylococcus aureus (mrsa) comparable with that of vancomycin. in this study, this substance had bacteriolytic activity against mrsa at 1 microg/ml as well as similar activity against vibrio parahaemolyticus at 24 microg/ml that suggests a novel antibacterial mode of action by this substance. heat and ph stability tests showed it ...	2003	12507841
the norm efflux pump of neisseria gonorrhoeae and neisseria meningitidis recognizes antimicrobial cationic compounds.	in neisseria gonorrhoeae and neisseria meningitidis, we identified a gene that would encode a protein highly similar to norm of vibrio parahaemolyticus (y. morita et al., antimicrob. agents chemother. 42:1778-1782, 1998). a nonpolar insertional mutation in either the gonococcal or meningococcal norm gene resulted in increased bacterial sensitivity to compounds harboring a quaternary ammonium on an aromatic ring (e.g., ethidium bromide, acriflavine hydrochloride, 2-n-methylellipticinium, and berb ...	2003	12533487
oyster-to-oyster variability in levels of vibrio parahaemolyticus.	this study examined the variability in the levels of total and pathogenic vibrio parahaemolyticus in individual oysters. twenty oysters were collected on three occasions (in june, july, and september 2001) from a site near mobile bay, ala. ten of these oysters were tested immediately, and 10 were tested after 24 h of storage at 26 degrees c. levels of total and pathogenic v. parahaemolyticus were determined by alkaline phosphatase-labeled dna probe procedures targeting the thermolabile hemolysin ...	2003	12540193
genome sequence of vibrio parahaemolyticus: a pathogenic mechanism distinct from that of v cholerae.	vibrio parahaemolyticus, a gram-negative marine bacterium, is a worldwide cause of food-borne gastroenteritis. v parahaemolyticus strains of a few specific serotypes, probably derived from a common clonal ancestor, have lately caused a pandemic of gastroenteritis. the organism is phylogenetically close to v cholerae, the causative agent of cholera.	2003	12620739
seasonal abundance of total and pathogenic vibrio parahaemolyticus in alabama oysters.	recent vibrio parahaemolyticus outbreaks associated with consumption of raw shellfish in the united states focused attention on the occurrence of this organism in shellfish. from march 1999 through september 2000, paired oyster samples were collected biweekly from two shellfish-growing areas in mobile bay, ala. the presence and densities of v. parahaemolyticus were determined by using dna probes targeting the thermolabile hemolysin (tlh) and thermostable direct hemolysin (tdh) genes for confirma ...	2003	12620838
the urease gene cluster of vibrio parahaemolyticus does not influence the expression of the thermostable direct hemolysin (tdh) gene or the tdh-related hemolysin gene.	in order to investigate why the thermostable direct hemolysin (tdh) and the tdh-related hemolysin (trh) of vibrio parahaemolyticus are produced at low levels from urease-positive strains, the effect of the functional urease gene cluster of v. parahaemolyticus on the expression of the tdh and trh genes was examined. transcriptional lacz fusions with the tdh1, tdh2, trh1 and trh2 genes representing variants of the tdh and trh genes were integrated into the chromosome of an escherichia coli strain ...	2003	12725294
bactericidal effect of tio2 photocatalyst on selected food-borne pathogenic bacteria.	titanium dioxide (tio(2)) photocatalysts have attracted great attention as a material for photocatalytic sterilization in the food and environmental industry. this research aimed to design a new photobioreactor and its application to sterilize selected food borne pathogenic bacteria, salmonella choleraesuis subsp., vibrio parahaemolyticus, and listeria monocytogenes. the photocatalytic reaction was carried out with various tio(2) concentrations and ultraviolet (uv) illumination time. a feasible ...	2003	12729712
a polar flagella operon (flg) of aeromonas hydrophila contains genes required for lateral flagella expression.	aeromonas spp. are pathogens of both humans and poikilothermic animals, causing a variety of diseases. certain strains are able to produce two distinct types of flagella; polar flagella for swimming in liquid and lateral flagella for swarming over surfaces. although, both types of flagella have been associated as colonisation factors, little is known about their organisation and expression. here we characterised a complete flagellar locus of aeromonas hydrophila (flg) containing 16 genes, this w ...	2003	12732473
application of polymerase chain reaction for detection of vibrio parahaemolyticus associated with tropical seafoods and coastal environment.	to study the incidence of vibrio parahaemolyticus in seafoods, water and sediment by molecular techniques vs conventional microbiological methods.	2003	12753253
vibrio parahaemolyticus infectious for both humans and edible mollusk abalone.	the aims of this study are to report evidence of the first laboratory-acquired infection of vibrio parahaemolyticus associated with handling experimentally infected abalones and to describe the virulence of the two bacterial strains tested in these animals. two strains of v. parahaemolyticus, one from the stool of a patient with acute gastroenteritis (strain 880713) and the other from the hemolymph of a diseased small abalone haliotis diversicolor supertexta (strain 880915), were identified and ...	2003	12758276
study of capsular polysaccharide from vibrio parahaemolyticus.	the leading cause of food poisoning in both taiwan and japan is vibrio parahaemolyticus infection, whose mechanism of enteropathogenesis is still unclear. to evaluate whether surface components are responsible for the intestinal adhesion of v. parahaemolyticus, we have developed a novel method for isolating the capsular polysaccharide (cps) from v. parahaemolyticus (serotype o4:k8). we found that culturing of v. parahaemolyticus in broth for 1 week or more changed the colony form of the bacteria ...	2003	12761115
response of vibrio parahaemolyticus 03:k6 to a hot water/cold shock pasteurization process.	vibrio vulnificus and v. parahaemolyticus are natural inhabitants of estuarine environments world wide. pathogenic strains of these bacteria are often transmitted to humans through consumption of raw oysters, which flourish in the same estuaries. previous studies reported the effective use of hot water pasteurization followed by cold shock to eliminate from raw oysters naturally and artificially incurred environmental strains of v. vulnificus and v. parahaemolyticus common to the gulf of mexico. ...	2003	12775474