Publications

[effect of bile on the development of tumors of the stomach and small intestine induced in rats by n-methyl-n-nitro-n-nitrosoguanidine].	the study of the influence of long-term injection of whole bile into rat's stomach on n-methyl-n-nitro-n-nitrosoguanidine-induced tumors revealed an increased frequency of stomach malignancies matched by a lower frequency of those in the small intestine as well as slower rates of growth of gastrointestinal tumors.	1987	3811314
proteolysis of v antigen from yersinia pestis.	lcr-plasmids of yersiniae are known to mediate a unique low calcium response characterised by restriction of growth in vitro with induction of putative virulence factors including yersiniae outer membrane-peptides (yops) and v antigen (lcr+). a medium was developed that permitted expression of high yields of v by yersinia pestis kim in large fermenter vessels. immunoblots of specific precipitates prepared by prior molecular sieving showed that native unaggregated v exists as a monomeric 37,000 d ...	1987	2853272
[wiedemann-beckwith syndrome: clinical characteristics, constitutional chromosome abnormalities and tumor incidence].	the observation of constitutional chromosome abnormalities in a one and half year old boy with wiedemann-beckwith syndrome (wbs) and hepatoblastoma prompted us to review the currently available literature dealing with this subject. in approximately 500 published cases with complete and incomplete forms of wbs, 42 malignant and 9 benign neoplasms have been described. malignant tumors, in particular, mainly consisted of nephroblastomas and adrenocortical carcinomas. the patient presented in this p ...	1987	2821320
cytomegalovirus infection in fifty-two renal transplant recipients.		1987	2856277
experimental transmission and pathogenesis of immunodeficiency syndrome in cats.	we describe the identification, experimental transmission, and pathogenesis of a naturally occurring powerfully immunosuppressive isolate of feline leukemia virus (designated here as felv-faids) which induces fatal acquired immunodeficiency syndrome (aids) in 100% (25 of 25) of persistently viremic experimentally infected specific pathogen-free (spf) cats after predictable survival periods ranging from less than 3 months (acute immunodeficiency syndrome) to greater than one year (chronic immunod ...	1987	2823940
experimental transmission and pathogenesis of immunodeficiency syndrome in cats.	we describe the identification, experimental transmission, and pathogenesis of a naturally occurring powerfully immunosuppressive isolate of feline leukemia virus (designated here as felv-faids) which induces fatal acquired immunodeficiency syndrome (aids) in 100% (25 of 25) of persistently viremic experimentally infected specific pathogen-free (spf) cats after predictable survival periods ranging from less than 3 months (acute immunodeficiency syndrome) to greater than one year (chronic immunod ...	1987	2823940
agonist-dependent phosphorylation of the alpha 2-adrenergic receptor by the beta-adrenergic receptor kinase.	desensitization of the beta-adrenergic receptor, a receptor which is coupled to the stimulation of adenylate cyclase, may be regulated via phosphorylation by a unique protein kinase. this recently discovered enzyme, known as the beta-adrenergic receptor kinase, only phosphorylates the agonist-occupied form of the beta-adrenergic receptor. to assess whether receptors coupled to the inhibition of adenylate cyclase might also be substrates, we examined the effects of beta-adrenergic receptor kinase ...	1987	2826414
reaction of tryptophanyl-trna synthetase from beef pancreas with periodate-oxidized atp.	tryptophanyl-trna synthetase from beef pancreas reacts with periodate-oxidized atp according to biphasic kinetics. a rapid phase involves two groups of the protein, presumably lysine side-chains. the slow phase corresponds to the reaction of a larger number of groups. the time-course of the partial losses of the atp-ppi isotopic exchange and of the aminoacylation activities of the enzyme follow the labelling of the two fast-reacting groups. however, the ability of the enzyme to form a bis(trypto ...	1987	2820730
comparative investigation of pmn leucocyte antimicrobial potential in animals of different species susceptibility to the plague agent.	the present work was aimed at a comparative study of oxygen-dependent metabolism (om) and myeloperoxidase (mpo) activities as well as the content of bactericidal cationic proteins (bcp) in polymorphonuclear leucocytes (pmnl) from mammals and birds with different susceptibility to plague (mice, guinea pigs, rats, great and miday gerbils, rabbits, dogs, pigeons and man). the experimental finding of an association between infectious susceptibility and functioning of the major pmnl bactericidal syst ...	1987	2824603
protein kinase c inhibitors eliminate hippocampal long-term potentiation.	recent findings suggest that protein kinase c (pkc) regulates the persistence of long-term potentiation (ltp). to test the hypothesis that pkc inhibition would decrease persistence of potentiation we applied pkc inhibitors (mellitin, polymyxin b, h-7) by micropressure ejection to the intact hippocampus either before or after ltp induction. when inhibitor was given 15 min before ltp, initial potentiation was unaffected, yet responses decayed to baseline levels by 60 min after the onset of potenti ...	1987	2825923
mucosal antibody response of calves after oral and intrabronchial administration of rotavirus.		1987	2827448
[incompatibility of the cad plasmid from yersinia pestis with plasmids of the incfi group].	the relation of yersinia pestis calcium dependence plasmid (pcad) to known inc fi (f'lac, r386, pox38) and incfv (f0lac) plasmids has been studied. evidence that plasmid pcad of yersinia pestis belongs to fi incompatibility group is presented.	1987	2828935
[studies on the hypothalamus-hypophyseal corticoliberin system. iii. effect of long-term administration of corticoliberin on serum levels of acth, tsh and thyroxine in male rats].		1987	2842125
antibodies to polymerized human serum albumin in acute and chronic liver disease.	since antibodies to polyalbumin have been noted to occur in patients with hepatitis or cirrhosis, we investigated sera from 219 patients with a variety of acute and chronic liver diseases with and without hbsag using an elisa. the prevalence of circulating polyalbumin antibodies was shown to be significantly increased over that of healthy controls (6.7%) in patients with autoimmune chronic active hepatitis (67%), acute viral hepatitis (41%) and fulminant hepatic necrosis (38%), but such antibodi ...	1987	2820857
false negative result by the wellcozyme anti-hiv assay in testing an hiv positive haemophiliac.		1987	2883508
effects of caffeine and cyclic adenosine 3',5'-monophosphate on adenosine triphosphate-dependent calcium uptake by lysed brain synaptosomes.	the adenosine triphosphate-dependent calcium uptake by endoplasmic reticulum elements of lysed synaptosomes from rat brain cortex was studied. caffeine exhibited a biphasic effect on this calcium uptake activity: concentrations of 1, 2, 5, 10 or 30 mm caffeine stimulated calcium uptake by 62, 111, 73, 88 and 60% respectively, whereas calcium uptake was inhibited by 55% at a 60-mm concentration of caffeine. calcium release from endoplasmic reticulum elements of lysed brain synaptosomes was stimul ...	1987	2825917
enhancement of contractile responses to partial alpha-adrenoceptor agonists during warming in rat aorta.	we examined the effects of warming on the contractile responses to full and partial alpha-adrenoceptor agonists in rat aorta. the contractions elicited by norepinephrine and methoxamine were not affected during warming (40 degrees c, 42 degrees c), whereas those induced by clonidine and st 587 were significantly enhanced. kcl-induced contractions of rat aorta were not affected by warming. the dissociation constants of clonidine and st 587 at 40 degrees c were not different from those at 37 degre ...	1987	2895427
development of aids, hiv seroconversion, and potential co-factors for t4 cell loss in a cohort of intravenous drug users.	a cohort of 334 intravenous (iv) drug users from new york city drug treatment programs were followed over a mean 9-month period. among the 165 who were seropositive at enlistment, four developed clinical aids, for an annual rate of 3%. elevated iga was a significant predictor of developing aids. among 72 subjects who were initially seronegative and who were re-interviewed, four were seropositive at follow-up, for a seroconversion rate of 7% per year among seronegatives. among seropositive subjec ...	1987	2896511
the effect of selective electrical stimulation of non-myelinated vagal fibres on heart rate in the rabbit.	the bradycardia evoked by electrical stimulation of the peripheral cut end of the rabbit vagus nerve is mediated by both myelinated and non-myelinated fibres. the purpose of this study was to assess the effects of non-myelinated fibres on heart rate in the rabbit using selective electrical stimulation techniques. in 8 rabbits selective activation of non-myelinated fibres using reversed polarity triangular shaped pulses (10 hz, 20 s), resulted in a slowly developing fall in heart rate of 24.1 +/- ...	1987	2897392
human papillomavirus dna in bronchial squamous cell carcinomas.		1987	2880009
avaii polymorphism in the human ldl receptor gene.		1987	2881256
polyester film as an advantageous tool for light and ultrastructural examination of single cells infected with viruses.	the use of marked transparent polyester film as a support of cell monolayer, smears or sections (cryostat or paraffin), makes possible to examine the same cell preselected in the light microscope by electron microscope (em). consequently, this technique enables to use histological or immunohistological investigations (using immunofluorescence or peroxidase (px) labelling) by light microscopy before examination in em. as example we used herpes simplex virus (hsv) type 1-infected cells grown on th ...	1987	2883863
a new subtype ("brezová") of tribec orbivirus (kemerovo group) isolated from ixodes ricinus males in czechoslovakia.		1987	2883865
antibodies to polymerized human serum albumin in acute and chronic liver disease.	since antibodies to polyalbumin have been noted to occur in patients with hepatitis or cirrhosis, we investigated sera from 219 patients with a variety of acute and chronic liver diseases with and without hbsag using an elisa. the prevalence of circulating polyalbumin antibodies was shown to be significantly increased over that of healthy controls (6.7%) in patients with autoimmune chronic active hepatitis (67%), acute viral hepatitis (41%) and fulminant hepatic necrosis (38%), but such antibodi ...	1987	2820857
chronic administration of sertraline, a selective serotonin uptake inhibitor, decreased the density of beta-adrenergic receptors in rat frontoparietal cortex.	sertraline, a potent and selective inhibitor of serotonin uptake, was chronically administered to laboratory rats. using in vitro receptor autoradiographic techniques, we found that the binding of [3h]dihydroalprenolol ([3h]dha) was reduced in cortex layers iv-vi. results of a saturation experiment indicated that the reduction in cortex layer iv was due to a change in number but not affinity of beta-adrenergic receptors.	1987	2825912
two distinct mechanisms for regulation of gamma-glutamyl transpeptidase in cultured rat hepatocytes by glucocorticoid-like steroids.	adult rat hepatocytes maintained in primary monolayer culture with defined medium were used to characterise two effects of glucocorticoid-like steroids in regulating gamma-glutamyltranspeptidase (ggt). low concentrations of glucocorticoids alone had little effect on ggt but synergistically enhanced induction of the enzyme by liver tumor-promoting xenobiotics such as 1,1,1-trichloro-2,2-bis-(4-chlorophenyl)-ethane (ddt) and hexachlorocyclohexane. the enhancing effect appears to be mediated by the ...	1987	2885003
[the spasmolytic activity of propiverine and some of its structural analogs].	the spasmolytic activity of propiverine and its corresponding methyl ether analogue was investigated in the isolated ileum and urinary bladder of the rat. it could be demonstrated that the antispasmodic properties of propiverine were, in contrast to its methyl ether analogue, not only based on a blockade of muscarinic receptors but were also a consequence of nonspecific papaverine-like effects. investigations with three putative metabolites showed that the alcohol derivative o-desalkylpropiverin ...	1987	2885006
falciparum malaria probably acquired from infected skin-cut.		1987	2885675
polymorphism of photopigments in the squirrel monkey: a sixth phenotype.	we describe here a trichromatic type of squirrel monkey that resembles old world monkeys in having two well-separated photopigments in the red-green part of the spectrum; the cones of this phenotype have peak sensitivities close to 430, 536 and 564 nm. the existence of such animals is predicted by a genetic model that postulates three alleles for a single locus on the x-chromosome of the squirrel monkey. the three alleles correspond to three different photopigments in the red-green spectral rang ...	1987	2888125
on the rate of f1-atpase turnover during atp hydrolysis by the single catalytic site. evidence that hydrolysis with a slow rate of product release does not occur at the alternating active site.	under conditions of molar excess of enzyme, isolated f1-atpase from beef heart mitochondria catalyses atp hydrolysis biphasically. the rate constants for product release are approximately 10(-1) and 10(-4)-10(-3) s-1, respectively. the slow phase of atp hydrolysis is insensitive to edta. [gamma-32p]atp splitting in the slow phase cannot be chased from the enzyme during several catalytic turnovers. it follows from these results that the slow single-site hydrolysis of atp (kcat approximately 10(-4 ...	1987	2888690
genetic study of piliation in escherichia coli: implications for understanding microbe-host interactions at the molecular level.		1987	2892189
chronic administration of sertraline, a selective serotonin uptake inhibitor, decreased the density of beta-adrenergic receptors in rat frontoparietal cortex.	sertraline, a potent and selective inhibitor of serotonin uptake, was chronically administered to laboratory rats. using in vitro receptor autoradiographic techniques, we found that the binding of [3h]dihydroalprenolol ([3h]dha) was reduced in cortex layers iv-vi. results of a saturation experiment indicated that the reduction in cortex layer iv was due to a change in number but not affinity of beta-adrenergic receptors.	1987	2825912
[the physiological effects of beta-adrenergic receptor stimulant on the rabbit lacrimal gland].		1987	2894752
[integrative nature of yersinia pestis virulence].		1987	2883791
loss of heterozygosity on chromosomes 3, 13, and 17 in small-cell carcinoma and on chromosome 3 in adenocarcinoma of the lung.	by a molecular genetic approach using polymorphic dna markers that detect allelic deletion of specific chromosomal regions, we analyzed for possible loss of chromosomal heterozygosity in five different histological types of lung cancers obtained from 47 patients. in small-cell carcinomas, the incidence of allelic deletions at three different chromosomal loci was extremely high; loss of heterozygosity was detected on chromosomes 3p in 7 of 7 patients (100%), 13q in 10 of 11 patients (91%), and 17 ...	1987	2892196
purification and immunological studies of selenoprotein a of the clostridial glycine reductase complex.	one of the essential catalytic components of the clostridial glycine reductase complex is a selenium-containing protein, selenoprotein a. an improved method for the purification of selenoprotein a has been developed that yields milligram quantities of the protein in a few relatively simple steps. ferredoxin and rubredoxin can be recovered in pure form as by-products of the procedure. the high resolving capabilities of an anion exchange high pressure liquid chromatography step were exploited in t ...	1987	2951374
neurosecretion of human hypothalamic immunoreactive beta-endorphin: in vitro regulation by dopamine.	an in vitro perifusion system was used to investigate immunoreactive beta-endorphin (beta-end-i) release from adult human hypothalami in response to dopamine (da) and the da receptor antagonist haloperidol (hal). administration of a 1 microm pulse of da consistently elicited a mean (+/- se) 88 +/- 9% increase (p less than 0.05, n = 5) in beta-end-i release, whereas 1 microm hal had no effect. administration of 1 microm da during three perifusions in which 1 microm hal was added to the medium fai ...	1987	2951610
novel approaches towards characterization of the high-affinity nucleotide binding sites on mitochondrial f1-atpase by the fluorescence probes 3'-o-(1-naphthoyl)adenosine di- and triphosphate.	the fluorescence properties of 3'-o-(1-naphthoyl)adenosine di- and triphosphates (termed n-adp and n-atp, respectively) were investigated in detail. of special importance for the use of these analogues as environmental probes is their high quantum yield (0.58 in water) and the polarity dependence of shape and wavelength position of the emission spectrum. upon binding of n-adp and n-atp to mitochondrial f1-atpase, the fluorescence intensity is markedly decreased, due to polarity changes and 'grou ...	1987	2883993
cytoplasmic calcium elevation in hippocampal granule cell induced by perforant path stimulation and l-glutamate application.	calcium-dependent fluorescence of a ca2+ indicator (fura-2) loaded in the slice of guinea pig hippocampus was measured by a microscope/video-camera/photometry system. tetanic stimulation of the perforant path (pp) or application of l-glutamate caused increment of the fluorescence from the dendritic and somatic layers of the granule cells in the dentate gyrus. magnitude of the increment depended on the frequency and intensity of the pp-stimulation or on the dose of l-glutamate. 2-aminophosphonova ...	1987	2884013
region-specific expression of two mouse homeo box genes.	mammalian homeo box genes have been identified on the basis of sequence homology to drosophila homeotic and segmentation genes. these studies examine the distribution of transcripts from two mouse homeo box genes, hox-2.1 and hox-3.1, throughout the latter third of prenatal development. transcripts from these genes are regionally localized along the rostro-caudal axis of the developing central nervous system, yielding expression patterns very similar to patterns of drosophila homeotic gene expre ...	1987	2881353
glutathione metabolizing enzyme activities in human thyroid.	glutathione peroxidases, glutathione transferase, glutathione reductase and gamma-glutamyl transpeptidase activities were analyzed in human thyroid tissues obtained from 17 patients undergoing resectional surgery because of a malignancy. it was deduced, from measurements of glutathione peroxidase activity with both h202 and cumene hydroperoxide, that thyroid contains only the selenium enzyme. the absence of selenium independent glutathione peroxidase activity in thyroid was confirmed with gel fi ...	1987	2883074
workers' education.		1987	2953594
workers' education.		1987	2953594
the insulin receptor tyrosyl kinase phosphorylates holomeric forms of the guanine nucleotide regulatory proteins gi and go.	an affinity purified human insulin receptor preparation was shown to phosphorylate the alpha- and beta-subunits of the guanine nucleotide-regulatory proteins gi and go, derived from bovine brain. the presence of insulin stimulated the rate of their phosphorylation some 2-fold. the presence of gi and go did not affect the degree of autophosphorylation of the beta-subunit of the insulin receptor. under conditions known to cause the dissociation of gi and go into their constituent subunits then pho ...	1987	3028864
the insulin receptor tyrosyl kinase phosphorylates holomeric forms of the guanine nucleotide regulatory proteins gi and go.	an affinity purified human insulin receptor preparation was shown to phosphorylate the alpha- and beta-subunits of the guanine nucleotide-regulatory proteins gi and go, derived from bovine brain. the presence of insulin stimulated the rate of their phosphorylation some 2-fold. the presence of gi and go did not affect the degree of autophosphorylation of the beta-subunit of the insulin receptor. under conditions known to cause the dissociation of gi and go into their constituent subunits then pho ...	1987	3028864
on the fidelity of dna replication: herpes dna polymerase and its associated exonuclease.	procaryotic dna polymerases contain an associated 3'----5' exonuclease activity which provides a proofreading function and contributes substantially to replication fidelity. dna polymerases of the eucaryotic herpes-type viruses contain similar associated exonuclease activities. we have investigated the fidelity of polymerases purified from wild type herpes simplex virus, as well as from mutator and antimutator strains. on synthetic templates, the herpes enzymes show greater relative exonuclease ...	1987	3029700
partial transformation of mouse fibroblastic and epithelial cell lines with the v-myc oncogene.	to investigate the role of the myc gene in mammalian cell transformation, plasmid constructs containing the v-myc oncogene and a co-selectable g418 resistance marker were introduced into both mouse fibroblasts (nih-3t3) and bladder epithelial cells (bbn3 and bbn7). after transfection or microinjection of dna, no transformed foci could be detected on confluent monolayers but, when the cells were cultured under conditions in which individual cells were allowed to grow and form colonies, morphologi ...	1987	3023119
[detection of an inhibitor of the causative agent of plague active against strains of the plague microbe from an homologous focus. the effect of lithium chloride on detecting the inhibitor].	a nutrient medium containing 0.7 per cent of lithium chloride was tested and yersinia pestis inhibitor was detected in 83.3 per cent of the strains on the growth tufts of 8 indicator strains isolated from a homologous focus. lowering the lithium chloride concentration in the medium up to 0.6 per cent promoted detection of the inhibitor in a larger number of the tested strains (93.3 per cent). but even on this medium none of the indicator strains formed overall growth tufts. the subcultures of th ...	1987	3032085
calmodulin in porcine malignant hyperpyrexia.	the anaesthetic complication malignant hyperpyrexia (mh) is due to an elevation of the myoplasmic ca2+ concentration. examination of calmodulin isolated from mh susceptible swine suggests that the disorder in calcium regulation in mh is not due to an abnormality in calmodulin.	1987	3032704
detection of antigen to herpes simplex virus in cerebrospinal fluid from patients with herpes simplex encephalitis.	cerebrospinal fluid (csf) specimens were obtained from patients with presumed herpes simplex encephalitis who underwent brain biopsy for diagnostic confirmation. coded csf specimens were fixed on nitrocellulose filter paper and probed with a pool of monoclonal antibodies directed against four herpes simplex virus glycoproteins (gb, gc, gd, and ge). herpes simplex virus antigen was detected in 35 of 40 specimens obtained from 26 biopsy-positive patients. in contrast, only three of 25 specimens fr ...	1987	3033092
the effects of selective dopamine receptor agonists and antagonists on body temperature in rats.	body temperature was measured at repeated time intervals following the administration of various dopamine agonists and antagonists. the d-1 and d-2 receptor agonist, apomorphine, produced dose-dependent hypothermia. this effect was inhibited by the d-2 receptor antagonist, spiroperidol. stimulation of d-2 receptor by ly171555 produced dose-dependent hypothermia, which was attenuated by pretreatment with spiroperidol and not altered by the d-1 receptor antagonist sch23390. the d-1 receptor agonis ...	1987	2951265
maturation antigen of the mouse sperm flagellum: ii. origin from holocrine cells of the distal caput epididymis.	during epididymal transit, the mouse sperm flagellum acquires a surface glycoprotein (sma4) from epididymal fluid that functions as a sperm antiagglutinin. to determine the origin of this molecule, testes and epididymides of male mice were sectioned for light microscopy and stained with wheat germ agglutinin (wga)-peroxidase, a probe that has been used previously to examine the biology of sma4. wga reactivity was localized to the cytoplasm in a small population of cells in the distal caput epidi ...	1987	3034104
fulminant leukemic polyradiculoneuropathy in a case of b-cell prolymphocytic leukemia. a clinicopathologic report.	a 52 year old man with a 10-month history of b-cell prolymphocytic leukemia (pll) died of an apparent acute fulminant polyradiculoneuropathy, a condition generally attributed to paraneoplastic complication. the pathologic examination disclosed diffuse leukemic infiltrations of the peripheral nervous system. it is suggested that this particularly aggressive form of b-cell chronic prolymphocytic leukemia presented a constellation of features that promoted the invasion of the peripheral nervous sys ...	1987	3040217
analysis of transfer genes and gene products within the trab-trac region of the escherichia coli fertility factor, f.	a series of plasmids that carry overlapping segments of f dna encoding the genes in the trab-trac interval was constructed, and a restriction enzyme map of the region was derived. plasmids carrying deletions that had been introduced at an hpai site within this interval were also isolated. the ability of these plasmids to complement transfer of f lac plasmids carrying mutations in trab, trav, and traw, and trac was analyzed. the protein products of the plasmids were labeled in uv-irradiated cells ...	1987	3040671
effects of procaine on pharmaco-mechanical coupling mechanisms activated by acetylcholine in smooth muscle cells of porcine coronary artery.	the action of procaine on pharmaco-mechanical coupling activated by application of acetylcholine (ach) was investigated using collagenase-treated dispersed intact and skinned smooth muscle cells and intact muscle tissues of the porcine coronary artery. ach reduced stored 45ca2+, and this action was prevented by procaine in intact dispersed cells. the maximum reduction in the level of stored 45ca induced by caffeine (25 mm) or inositol 1,4,5-trisphosphate (insp3; 3 microm) was also prevented by p ...	1987	3034448
a factor discriminating between the wild-type and a mutant polyomavirus enhancer.	enhancers increase the frequency of transcription initiation from linked promoter elements, most probably as a result of the binding of specific proteins to the enhancer. the polyomavirus early region is expressed in differentiated mouse cells but not in undifferentiated embryonal carcinoma (ec) cells. this host range is a function of the enhancer because polyomavirus mutants selected for growth in ec cells have mutations in the enhancer and the host range is reproduced in transfection assays us ...	1987	3037380
the pharmacokinetics of proguanil in human subjects following a single oral dose.	the pharmacokinetics of orally administered 200-mg dose of proguanil in volunteers, one african and one caucasian, is described. the drug was rapidly absorbed reaching a peak concentration in the blood within 3 h, and declining slowly thereafter to give a terminal phase elimination half life of 11.20 +/- 4.10 h and a systemic clearance of 1.270 +/- 0.020 l/h/kg. the small apparent volume of distribution shows that the drug is confined mainly to the blood and is not extensively bound to tissues; ...	1987	3037875
cloning in escherichia coli of the gene specifying the dna-entry nuclease of bacillus subtilis.	with the aim of cloning genes involved in transformation of bacillus subtilis, a set of transformation-deficient mutants was isolated by means of insertional mutagenesis with plasmid phv60 (vosman et al., 1986). analysis of these mutants showed that those mapping in the aroi region lacked the dna-entry nuclease activity. plasmid phv60 derivatives, containing flanking chromosomal dna fragments, were isolated from these mutants and were used to screen a library of b. subtilis chromosomal dna in ph ...	1987	3038682
cloning in escherichia coli of the gene specifying the dna-entry nuclease of bacillus subtilis.	with the aim of cloning genes involved in transformation of bacillus subtilis, a set of transformation-deficient mutants was isolated by means of insertional mutagenesis with plasmid phv60 (vosman et al., 1986). analysis of these mutants showed that those mapping in the aroi region lacked the dna-entry nuclease activity. plasmid phv60 derivatives, containing flanking chromosomal dna fragments, were isolated from these mutants and were used to screen a library of b. subtilis chromosomal dna in ph ...	1987	3038682
the effect of cycloleucine on sfv a7(74) infection in mice.	cycloleucine (cl), a non-metabolizable amino acid analogue, was found to reduce thymus and spleen weights in semliki forest virus (sfv) strain a7(74) infected and control mice. the maximum effects were seen when three daily doses of cl were given to mice 24 h after an sfv a7(74) infection. in these mice thymus atrophy led to abolition of thymus dependent immune responses and changes in the pathological features of the viral infection--the most striking feature being prevention of demyelination. ...	1987	3034321
plasmid-mediated virulence in yersiniae.		1987	3078833
membrane conductance and potassium permeability of the rat lens.	the membrane potential, electrical impedance and 86rb+ efflux rate constants were measured in the rat lens perifused at 35 degrees c. the membrane conductance was obtained from the difference between the magnitude of the impedance at low (less than 0.1 hz) and high (greater than 100 hz) frequencies. values of the rubidium permeability coefficients (prb) were obtained from the rate constant and potential data. the values for the membrane potential and conductance in control solution (5 mm potassi ...	1987	3031720
open-chest cardiac massage after closed-chest compression in a canine model: when to intervene.	open-chest cardiac massage appears beneficial in improving hemodynamics during resuscitation efforts and in improving resuscitation success. the time between cardiac arrest and the initiation of open-chest cardiac massage is crucial. it would appear that if initiation of open chest cardiac massage is delayed for more than 20 min from the onset of cardiac arrest, little or no successful outcome can be expected. further techniques for assessing the adequacy of closed-chest compression cpr are need ...	1987	3035670
cdna cloning of human myeloperoxidase: decrease in myeloperoxidase mrna upon induction of hl-60 cells.	myeloperoxidase (mpo), the most abundant neutrophil protein, is a bacteriocidal component of the primary granules and a critical marker in distinguishing acute myelogenous leukemia from acute lymphoid leukemia. a cdna clone for human mpo was isolated by immunologic screening of human hematopoietic lambda gt11 expression vector libraries with specific anti-mpo antibody. the identity of the cdna clone was confirmed by finding that epitope-selected antibody against this clone recognizes purified mp ...	1987	3031662
procollagenase activator produced by rabbit uterine cervical fibroblasts.	culture medium from rabbit uterine cervical fibroblasts contained a procollagenase and a neutral proproteinase which acts as a procollagenase activator. these two proenzymes have been purified by a combination of ion-exchange, affinity and gel chromatographies. the purified neutral proproteinase showed mr 60,000 with sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. this neutral proproteinase was activated by trypsin, 4-aminophenylmercuric acetate (apma) and plasmin, and the active spe ...	1987	3036065
antagonist effects of the enantiomers of 3-ppp towards alpha 1-adrenoceptors coupled to inositol phospholipid breakdown in the rat cerebral cortex.	the effects of the two enantiomers of 3-ppp upon alpha 1-adrenergic and muscarinic receptors coupled to the inositol phospholipid (pi) breakdown response have been investigated. 3-ppp(-) and 3-ppp(+) were found to antagonize the noradrenaline (10 microm)-stimulated pi breakdown in rat cerebral cortical miniprisms with ic50 values of 18 and 61 microm, respectively. the dopamine receptor antagonists haloperidol and raclopride were also antagonists, with ic50 values of 0.4 and 25 microm, respective ...	1987	3039482
membrane conductance and potassium permeability of the rat lens.	the membrane potential, electrical impedance and 86rb+ efflux rate constants were measured in the rat lens perifused at 35 degrees c. the membrane conductance was obtained from the difference between the magnitude of the impedance at low (less than 0.1 hz) and high (greater than 100 hz) frequencies. values of the rubidium permeability coefficients (prb) were obtained from the rate constant and potential data. the values for the membrane potential and conductance in control solution (5 mm potassi ...	1987	3031720
cdna cloning of human myeloperoxidase: decrease in myeloperoxidase mrna upon induction of hl-60 cells.	myeloperoxidase (mpo), the most abundant neutrophil protein, is a bacteriocidal component of the primary granules and a critical marker in distinguishing acute myelogenous leukemia from acute lymphoid leukemia. a cdna clone for human mpo was isolated by immunologic screening of human hematopoietic lambda gt11 expression vector libraries with specific anti-mpo antibody. the identity of the cdna clone was confirmed by finding that epitope-selected antibody against this clone recognizes purified mp ...	1987	3031662
nocardia asteroides as a cause of corneal ulcer. case report.		1987	3551895
elimination of 131i in meadow voles (microtus pennsylvanicus) measured by whole-body gamma spectrometry.		1987	3553074
[pathogenic properties of yersinia and their role in yersiniosis pathology].		1987	3554847
rhodopsin, vitamin a, and interstitial retinol-binding protein in the rd chicken.	in order to determine whether blindness in the rd strain of rhode island red chickens is due to a defect in the vitamin a (visual) cycle, spectroscopy, high performance liquid chromatography, and immunochemical techniques were used to compare the amounts of rhodopsin, interstitial retinol-binding protein, and vitamin a compounds in the dark-adapted eyes of homozygous rd and heterozygous carriers. in both groups of chickens, (up to 6 weeks post-hatching) the distribution of stored vitamin a diffe ...	1987	3557867
phase dynamics at microtubule ends: the coexistence of microtubule length changes and treadmilling.	the length dynamics both of microtubule-associated protein (map)-rich and map-depleted bovine brain microtubules were examined at polymer mass steady state. in both preparations, the microtubules exhibited length redistributions shortly after polymer mass steady state was attained. with time, however, both populations relaxed to a state in which no further changes in length distributions could be detected. shearing the microtubules or diluting the microtubule suspensions transiently increased th ...	1987	3558477
a cytosolic protein contains a cryptic mitochondrial targeting signal.	cytosolic dihydrofolate reductase from mouse contains a cryptic mitochondrial targeting sequence. if this sequence is attached to the amino terminus of 'passenger' proteins which by themselves cannot enter mitochondria, the resulting fusion proteins are transported into yeast mitochondria.	1987	3543689
monohydroperoxides of linoleic acid in endoplasmic lipids of rats exposed to tetrachloromethane.	an accurate method for the quantitative determination of hydroperoxy and hydroxy fatty acids in liver microsomes is presented which involves the use of 18o-labeled internal standards. the method is employed for the determination of hydroperoxides in rat liver microsomes after aerobic incubation with fe2+/adp and in microsomes from animals exposed to 75 mg tetrachloromethane/kg body weight. the amounts found after artificial microsomal "lipid peroxidation" are almost two orders of magnitude large ...	1987	3431344
plague meningitis--a retrospective analysis of cases reported in the united states, 1970-1979.	meningitis caused by yersinia pestis developed in 6 (6%) of a total of 105 patients with plague reported to the centers for disease control from 1970 to 1979. five of the six cases occurred in children aged 10 to 15 years. all six patients received antibiotic therapy before meningitis developed, which appeared between the 9th and 14th days after the onset of acute illness in five of the six patients. there were no neurologic sequelae. the antigenic and biochemical profiles of the y pestis strain ...	1987	3424819
[adenylate cyclase of the causative agent of plague: its purification and properties].	three forms of adenylate cyclase have been detected in y. pestis: membrane-bound, cytoplasmic and extracellular. extracellular adenylate cyclase has been purified so as to achieve a homogeneous state, and some of its physicochemical parameters have been investigated. in the process of purification the initial preparation of this enzyme has been subjected to heating at 100 degrees c for 15 minutes, fractionation with ammonium sulfate, and gel filtration on sephadex g-100. the homogeneity of adeny ...	1987	3445726
antibacterial activity of amniotic fluid in the early third trimester. its association with preterm labor and delivery.	the purpose of this study was to determine the bacterial growth inhibitory property of amniotic fluid (af) from patients in the early third trimester and to examine its relationship to preterm labor and delivery. a total of 38 af samples from patients between 27 and 35 weeks gestation with intact membranes were studied. antibacterial activity was tested against escherichia coli, staphylococcus aureus, and bacteroides fragilis. fifteen fluids were from patients in preterm labor who delivered near ...	1987	3539135
enzyme activity changes in rat soleus motoneurons and muscle after synergist ablation.	quantitative enzyme histochemical methods have been used to determine the effect of ablation of synergists on the oxidative metabolism of the alpha-motoneurons and muscle fibers of the rat soleus. sixty days postablation, the nadh-tetrazolium reductase (nadh-tr) activity of soleus motoneurons decreased 12.5% from 0.327 +/- 0.005 (mean +/- se; optical density units) to 0.286 +/- 0.007. in the muscle fibers, the alpha-glycerophosphate dehydrogenase activity (glycolytic enzyme) decreased from 0.114 ...	1987	3436865
effects of pomc-derived peptides on aldosterone secretion in vivo.	1. to investigate a role for peptides derived from the precursor molecule pro-opiomelanocortin (pomc) on the control of aldosterone secretion (asr), alpha-, beta-, gamma 1-, and gamma 2-melanocyte stimulating hormone (msh), corticotropin-like intermediate lobe peptide (clip) or beta-endorphin were infused into the adrenal arterial supply of sheep with an adrenal cervical autotransplant. 2. none of the peptides had any significant effect on aldosterone secretion rate in na replete, unstressed, co ...	1987	3440325
difference in monoamine oxidase b activity between c57 black and albino nmri mouse strains may explain differential effects of the neurotoxin mptp.	monoamine oxidase b (mao-b) is the key enzyme in the conversion of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(mptp) to n-methyl-4-phenyl-pyridinium ion (mpp+) which causes degeneration of dopaminergic nigral neurons. using a histochemical tetrazolium method for mao-b with tyramine as substrate and chlorgyline for the inhibition of mao-a, black c57 mice were found to have a higher brain mao-b activity than similar aged albino nmri mice. the difference, which was in general density rather than d ...	1987	3498133
sharing of mhc haplotypes among apparently unrelated patients with congenital adrenal hyperplasia due to 21-hydroxylase deficiency.	from the study of hla, complement, and glyoxalase i alleles in 82 venezuelan individuals belonging to 19 families of mixed ethnic origin having 20 affected newborns with salt-wasting congenital adrenal hyperplasia due to 21-hydroxylase (21-oh) deficiency, a total of 38 disease haplotypes and 53 nondisease haplotypes were found. of the pathological haplotypes 47% were found to share the hla-b39 or -bw62 specificities, 55% of them in combination with the bfs, c2c, c4a4, c4b2 (sc42) complotype. the ...	1987	3493216
yersinia pestis endophthalmitis.		1987	3495184
dexamethasone pre-treatment is antipyretic toward polyinosinic: polycytidylic acid, lipopolysaccharide and interleukin 1/endogenous pyrogen.	the effect of intravenously injected dexamethasone on the febrile response of rabbits to polyinosinic: polycytidylic acid (poly i:c), lipopolysaccharide (lps) and interleukin 1/endogenous pyrogen (il1/e.p.) was studied. dexamethasone (1 mg/kg) attenuated the febrile response to poly i:c (5 micrograms/kg) but only if administered between 0.5 to 2 h before poly i:c. if it was given after poly i:c this resulted in a potentiation of the fever. antagonism of the febrile response to poly i:c by dexame ...	1987	3495738
antigenic and amino acid sequence analysis of the variants of h1n1 influenza virus in 1986.	since their reintroduction to human populations in 1977, influenza a viruses of the h1n1 subtype have undergone antigenic drift. recently a distinct antigenic variant, a/singapore/6/86, has been almost exclusively isolated internationally, and the antigenic properties and amino acid sequence of its haemagglutinin have been determined and compared with those of the haemagglutinins of other h1n1 viruses, in particular a/chile/1/83. fourteen amino acid sequence differences are detected between the ...	1987	3496983
local cerebral metabolic effects of l-dopa therapy in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced parkinsonism in monkeys.	the quantitative 2-deoxy[14c]glucose autoradiographic method was used to map the distribution of alterations in local cerebral glucose utilization that accompanies clinically effective chronic l-dopa therapy of rhesus monkeys made parkinsonian by the administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (mptp). this pattern of changes was compared to the effects of a similar treatment regimen in normal monkeys. l-dopa (100 mg with 10 mg carbidopa) was administered orally to normal and p ...	1987	3497401
effects of dietary fish oil on renal insufficiency in rats with subtotal nephrectomy.	we studied the effects of fish oil on the progression of renal insufficiency in rats with subtotal nephrectomy. five weeks after a 1-2/3 nephrectomy, sixteen rats were fed two different diets which differed only in fat composition. lipid in the control diet was primarily beef tallow; that of the experimental diet, menhaden oil. fish oil-fed rats had significant increases in plasma creatinines, decreases in urinary pge2 and accelerated death rates. an additional twelve rats underwent 1-1/3 nephre ...	1987	3480973
treatment of cutaneous sclerosis and aplastic anemia with antithymocyte globulin.	we administered antithymocyte globulin to a 52-year-old man with cutaneous sclerosis (scleroderma) and severe aplastic anemia for treatment of the aplastic lesion. the use of this therapy resulted not only in marrow recovery but also in resolution of the sclerosis. this observation indicates that t lymphocytes, their products, or both, are important factors in the pathogenesis of cutaneous sclerosis. furthermore, the success of this treatment could play a role in future investigations involving ...	1987	3491559
vaccination against cutaneous leishmaniasis in a murine model. ii. immunologic properties of protective and nonprotective subfractions of soluble promastigote extract.	we have previously demonstrated that balb/c mice can be protected against a fatal infection with leishmania major by i.p. immunization with a soluble leishmanial antigen (sla) preparation in conjunction with the adjuvant, corynebacterium parvum (cp). in this study, sla was separated into nine distinct fractions by anion exchange liquid chromatography, and the fractions were analyzed for their ability to stimulate t cells obtained from immunized mice, to be recognized by vaccine-induced antibodie ...	1987	3499465
biphasic effects of epidermal growth factor on bone nodule formation by isolated rat calvaria cells in vitro.	osteoblast-like cells isolated from fetal rat calvaria (rc) form three-dimensional nodules in vitro having the morphological and immunohistochemical characteristics of bone. when administered continually over 21 days of culture, egf caused a dose-related inhibition of nodule formation, as well as a reduction in the proportion of mineralized nodules, at concentrations between 10(-12) to 10(-7) m. the same conditions caused an increase in both cell proliferation and saturation density in the cultu ...	1987	3502681
biphasic effects of epidermal growth factor on bone nodule formation by isolated rat calvaria cells in vitro.	osteoblast-like cells isolated from fetal rat calvaria (rc) form three-dimensional nodules in vitro having the morphological and immunohistochemical characteristics of bone. when administered continually over 21 days of culture, egf caused a dose-related inhibition of nodule formation, as well as a reduction in the proportion of mineralized nodules, at concentrations between 10(-12) to 10(-7) m. the same conditions caused an increase in both cell proliferation and saturation density in the cultu ...	1987	3502681
post-translational regulation of lcr plasmid-mediated peptides in pesticinogenic yersinia pestis.	the low calcium response of wild type yersinia pestis, the causative agent of bubonic plague, and of enteropathogenic yersinia pseudotuberculosis and yersinia enterocolitica is known to be mediated by a shared lcr plasmid of about 70 kb. at 37 degrees c in ca2+-deficient medium, this element promotes restriction of growth with concomitant production of virulence functions including the common v antigen and a set of yersiniae outer membrane peptides termed yops (lcr+). the latter are expressed by ...	1987	3504547
modulation of the low-calcium response in yersinia pestis via plasmid-plasmid interaction.	virulent cells of yersinia pestis, yersinia pseudotuberculosis, and yersinia enterocolitica are known to exhibit a low-calcium response in vitro characterized by restriction of growth and induction of v antigen at 37 degrees c in ca2+-deficient media (lcr+). a shared lcr plasmid mediates these properties and encodes yersiniae outer membrane peptides (yops) that are expressed in lcr+ y. pseudotuberculosis and y. enterocolitica but not y. pestis. we present direct evidence here verifying that synt ...	1987	3507558
[the utilization of iron of various substrates by yersinia pestis].		1987	3508925
the biochemical basis of niridazole toxicity.		1987	3509430
induction and activity of several isoenzymes of cytochrome p-450 in primary cultures of rat hepatocytes, in comparison with in vivo data.	changes in cytochrome p-450 isoenzymes were studied in rat liver and in primary cultures of rat hepatocytes after treatment with compounds belonging to various classes of inducers, including phenobarbital (pb), beta-naphthoflavone (bnf), and clofibrate/clofibric acid (clof/clofa). the enzyme activity toward specific substrates was measured, and the presence of apoprotein of several p-450 isoenzymes was determined semiquantitatively by western blotting. in untreated cultures the p-450 content and ...	1987	3509691
regional variations in oral submucous fibrosis in india.	regional variations in the characteristics of submucous fibrosis were studied in two districts in india. in pune district this condition involved soft palate, uvula and retromolar areas significantly more often than in ernakulam district. the tongue, floor of the mouth and the hard palate were not involved in pune. the age of the patients in pune district was lower than in ernakulam district. associated oral cancer, leukoplakia and petechiae were observed solely among patients in ernakulam. the ...	1987	3476249
[chromosome stability in saccharomycete yeasts].	mutants with high instability of chromosome iii designated chl+ (chromosome loss) were obtained after irradiation with uv the z4221-3c1 haploid disomic for chromosome iii. the chl+ mutants can be divided into two classes: 1) cl2, cl3, cl7, cl9, cl11, cl12, cl13 with elevated level of spontaneous inter- and intragenic recombination; 2) cl4, cl8 which unstable maintenance of chromosome iii not accompanied with elevation of mitotic recombination frequency. the cl4 and cl8 mutants also reveal, in co ...	1987	3326785
fetal heart rate, arterial pressure, and blood volume responses to cortisol infusion.	the purpose of this study was to determine whether physiological amounts of cortisol affect the fetal cardiovascular system. cortisol (4 micrograms/min) or the vehicle was infused intravenously for 5 h into six chronically catheterized sheep fetuses at 127-143 days gestation (term = 145-150 days). in the cortisol-infused animals, plasma cortisol concentration increased from 2.0 +/- 0.6 (se) to 8.3 +/- 0.9 ng/ml. there was a concomitant decrease in fetal heart rate of 38 beats/min (p less than 1 ...	1987	3322047
mode of action of nitro-heterocyclic compounds on escherichia coli.	the inhibitory and bactericidal activities of several different nitro-heterocyclic compounds, such as nitrofuran, nitronaphthofuran, nitrobenzofuran, nitroimidazole and nitrothiazole, were assessed in vitro. all these substances except nitroimidazole were active against escherichia coli, though to different degrees. under anaerobic test conditions the antibacterial activity increased slightly. nitroreductase-deficient mutants, however, were highly resistant to all nitro-compounds, indicating tha ...	1987	3322751