Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| microbiological efficacy of photodynamic therapy as an adjunct to non-surgical periodontal treatment: a clinical trial. | the efficiency of routine scaling and root planning is negatively influenced by the tooth anatomy and residual bacteria all possibly affecting the treatment outcomes in future. the present study compared the microbiologic effectiveness of the photodynamic therapy (pdt) as an adjunctive treatment modality for nonsurgical treatment in chronic periodontitis. | 2016 | 27330709 |
| oxidative stress and periodontal disease in obesity. | periodontal disease is a chronic inflammatory disease of the jaws and is more prevalent in obesity. local and systemic oxidative stress may be an early link between periodontal disease and obesity. the primary aim of this study was to detect whether increased periodontal disease susceptibility in obese individuals is associated with local and systemic oxidative stress. accordingly; we analyzed periodontal status and systemic (serum) and local (gingival crevicular fluid [gcf]) oxidative status ma ... | 2016 | 27015191 |
| the outer-membrane export signal of porphyromonas gingivalis type ix secretion system (t9ss) is a conserved c-terminal β-sandwich domain. | in the recently characterized type ix secretion system (t9ss), the conserved c-terminal domain (ctd) in secreted proteins functions as an outer membrane translocation signal for export of virulence factors to the cell surface in the gram-negative bacteroidetes phylum. in the periodontal pathogen porphyromonas gingivalis, the ctd is cleaved off by poru sortase in a sequence-independent manner, and anionic lipopolysaccharide (a-lps) is attached to many translocated proteins, thus anchoring them to ... | 2016 | 27005013 |
| c/ebp β mediates endoplasmic reticulum stress regulated inflammatory response and extracellular matrix degradation in lps-stimulated human periodontal ligament cells. | periodontitis is an oral inflammatory disease that not only affects the integrity of local tooth-supporting tissues but also impacts systemic health. a compositional shift in oral microbiota has been considered as the main cause of periodontitis; however, the potential mechanism has not been fully defined. herein, we investigated the role of ccaat/enhancer-binding protein β (c/ebp β), a member of the c/ebp family of transcription factors, in human periodontal ligament cells (hpdlcs) exposed to p ... | 2016 | 27011164 |
| reduced glutathione mediates resistance to h2s toxicity in oral streptococci. | periodontal disease is associated with changes in the composition of the oral microflora, where health-associated oral streptococci decrease while gram-negative anaerobes predominate in disease. a key feature of periodontal disease-associated anaerobes is their ability to produce hydrogen sulfide (h2s) abundantly as a by-product of anaerobic metabolism. so far, h2s has been reported to be either cytoprotective or cytotoxic by modulating bacterial antioxidant defense systems. although oral anaero ... | 2016 | 26801579 |
| systematic engineering of the central metabolism in escherichia coli for effective production of n-butanol. | microbes have been extensively explored for production of environment-friendly fuels and chemicals. the microbial fermentation pathways leading to these commodities usually involve many redox reactions. this makes the fermentative production of highly reduced products challenging, because there is a limited nadh output from glucose catabolism. microbial production of n-butanol apparently represents one typical example. | 2016 | 26997975 |
| oral microbiota species in acute apical endodontic abscesses. | acute apical abscesses are serious endodontic diseases resulting from pulpal infection with opportunistic oral microorganisms. the objective of this study was to identify and compare the oral microbiota in patients (n=18) exhibiting acute apical abscesses, originating from the demographic region in portland, oregon. the study hypothesis is that abscesses obtained from this demographic region may contain unique microorganisms not identified in specimens from other regions. | 2016 | 26983837 |
| microrna expression profile of human periodontal ligament cells under the influence of porphyromonas gingivalis lps. | periodontitis is a chronic inflammatory disease which is caused by bacterial infection and leads to the destruction of periodontal tissues and resorption of alveolar bone. thus, special attention should be paid to the mechanism under lipopolysaccharide (lps)-induced periodontitis because lps is the major cause of periodontitis. however, to date, mirna expression in the lps-induced periodontitis has not been well characterized. in this study, we investigated mirna expression patterns in lps-treat ... | 2016 | 26987780 |
| genome-wide association study of biologically informed periodontal complex traits offers novel insights into the genetic basis of periodontal disease. | genome-wide association studies (gwas) of chronic periodontitis (cp) defined by clinical criteria alone have had modest success to-date. here, we refine the cp phenotype by supplementing clinical data with biological intermediates of microbial burden (levels of eight periodontal pathogens) and local inflammatory response (gingival crevicular fluid il-1β) and derive periodontal complex traits (pcts) via principal component analysis. pcts were carried forward to gwas (∼2.5 million markers) to iden ... | 2016 | 26962152 |
| microbial diversity in the early in vivo-formed dental biofilm. | although the mature dental biofilm composition is well studied, there is very little information on the earliest phase of in vivo tooth colonization. progress in dental biofilm collection methodologies and techniques of large-scale microbial identification have made new studies in this field of oral biology feasible. the aim of this study was to characterize the temporal changes and diversity of the cultivable and noncultivable microbes in the early dental biofilm. samples of early dental biofil ... | 2016 | 26746720 |
| antibacterial activity and mechanism of action of auranofin against multi-drug resistant bacterial pathogens. | traditional methods employed to discover new antibiotics are both a time-consuming and financially-taxing venture. this has led researchers to mine existing libraries of clinical molecules in order to repurpose old drugs for new applications (as antimicrobials). such an effort led to the discovery of auranofin, a drug initially approved as an anti-rheumatic agent, which also possesses potent antibacterial activity in a clinically achievable range. the present study demonstrates auranofin's antib ... | 2016 | 26936660 |
| hand to mouth: a systematic review and meta-analysis of the association between rheumatoid arthritis and periodontitis. | rheumatoid arthritis (ra) and periodontitis are both chronic inflammatory diseases, which demonstrate similarities in terms of mechanism, histopathology, and demography. an association between these conditions has been demonstrated previously but has been called into question more recently. | 2016 | 26973655 |
| detection of cfxa2, cfxa3, and cfxa6 genes in beta-lactamase producing oral anaerobes. | purpose the aim of this study was to identify β-lactamase-producing oral anaerobic bacteria and screen them for the presence of cfxa and blatem genes that are responsible for β-lactamase production and resistance to β-lactam antibiotics. material and methods periodontal pocket debris samples were collected from 48 patients with chronic periodontitis and anaerobically cultured on blood agar plates with and without β-lactam antibiotics. presumptive β-lactamase-producing isolates were evaluated for ... | 2016 | 27119762 |
| comparison of the efficacy of subgingival irrigation with 2% povidone-iodine and tetracycline hcl in subjects with chronic moderate periodontitis: a clinico microbiological study. | this study was performed to evaluate and compare the clinical and antimicrobial efficacy of subgingival irrigation with tetracycline and povidone-iodine as an adjunct to nonsurgical periodontal therapy. | 2016 | 27076823 |
| microbiological and clinical effects of sitafloxacin and azithromycin in periodontitis patients receiving supportive periodontal therapy. | sitafloxacin (stfx) is a newly developed quinolone that has robust antimicrobial activity against periodontopathic bacteria. we previously reported that oral administration of stfx during supportive periodontal therapy was as effective as conventional mechanical debridement under local anesthesia microbiologically and clinically for 3 months. the aim of the present study was to examine the short-term and long-term microbiological and clinical effects of systemic stfx and azithromycin (azm) on ac ... | 2016 | 26729495 |
| neutrophil functions in periodontal homeostasis. | oral tissues are constantly exposed to damage from the mechanical effort of eating and to microorganisms, mostly bacteria. in healthy gingiva tissue remodeling and a balance between bacteria and innate immune cells are maintained. however, excess of bacteria biofilm (plaque) creates an inflammation state that recruits more immune cells, mainly neutrophils to the gingiva. neutrophils create a barrier for bacteria to reach inside tissues. when neutrophils are insufficient, bacteria thrive causing ... | 2016 | 27019855 |
| management of diabolical diabetes mellitus and periodontitis nexus: are we doing enough? | periodontitis is the commonest oral disease affecting population worldwide. this disease is notorious for the devastation of tooth supporting structures, ensuing in the loss of dentition. the etiology for this disease is bacterial biofilm, which accumulates on the teeth as dental plaque. in addition to the biofilm microorganisms, other factors such as environmental, systemic and genetic are also responsible in progression of periodontitis. diabetes mellitus (dm) is metabolic disorder which has a ... | 2016 | 26962409 |
| n-butanol production in saccharomyces cerevisiae is limited by the availability of coenzyme a and cytosolic acetyl-coa. | butanol isomers are regarded as more suitable fuel substitutes than bioethanol. n-butanol is naturally produced by some clostridia species, but due to inherent problems with clostridial fermentations, industrially more relevant organisms have been genetically engineered for n-butanol production. although the yeast saccharomyces cerevisiae holds significant advantages in terms of scalable industrial fermentation, n-butanol yields and titers obtained so far are only low. | 2016 | 26913077 |
| bacterial diversity in saliva and oral health-related conditions: the hisayama study. | this population-based study determined the salivary microbiota composition of 2,343 adult residents of hisayama town, japan, using 16s rrna gene next-generation high-throughput sequencing. of 550 identified species-level operational taxonomic units (otus), 72 were common, in ≥75% of all individuals, as well as in ≥75% of the individuals in the lowest quintile of phylogenetic diversity (pd). these "core" otus constituted 90.9 ± 6.1% of each microbiome. the relative abundance profiles of 22 of the ... | 2016 | 26907866 |
| identification of known and novel recurrent viral sequences in data from multiple patients and multiple cancers. | virus discovery from high throughput sequencing data often follows a bottom-up approach where taxonomic annotation takes place prior to association to disease. albeit effective in some cases, the approach fails to detect novel pathogens and remote variants not present in reference databases. we have developed a species independent pipeline that utilises sequence clustering for the identification of nucleotide sequences that co-occur across multiple sequencing data instances. we applied the workf ... | 2016 | 26907326 |
| influence of type 2 diabetes on prevalence of key periodontal pathogens, salivary matrix metalloproteinases, and bone remodeling markers in sudanese adults with and without chronic periodontitis. | this study compared the influence of type 2 diabetes on the occurrence of six periodontal pathogens in plaque samples of patients with and without chronic periodontitis. levels of salivary mmp-8, mmp-9, rankl, and opg were also investigated. the study enrolled 31 patients with type 2 diabetes and chronic periodontitis (dm + cp), 29 with chronic periodontitis (cp), and 20 with type 2 diabetes (dm). questionnaire-guided interviews were conducted and plaque index, bleeding on probing, and pocket de ... | 2016 | 26989414 |
| prevalence of porphyromonas gingivalis fima genotypes in the peri-implant sulcus of koreans assessed using a new primer. | porphyromonas gingivalis fima is a virulence factor associated with periodontal diseases, but its role in the pathogenesis of peri-implantitis remains unclear. we aimed to evaluate the relationship between the condition of peri-implant tissue and the distribution of p. gingivalis fima genotypes in koreans using a new primer. | 2016 | 26937292 |
| surface interactions between two of the main periodontal pathogens: porphyromonas gingivalis and tannerella forsythia. | porphyromonas gingivalis and tannerella forsythia have been implicated as the major etiologic agents of periodontal disease. these two bacteria are frequently isolated together from the periodontal lesion, and it has been suggested that their interaction may increase each one's virulence potential. the purpose of this study was to identify proteins on the surface of these organisms that are involved in interbacterial binding. | 2016 | 26937289 |
| role of nitric oxide in the pathogenesis of barrett's-associated carcinogenesis. | barrett's esophagus (be), a premalignant condition to barrett's adenocarcinoma (bac), is closely associated with chronic inflammation due to gastro-esophageal reflux. caudal type homeobox 2 (cdx2), a representative marker of be, is increased during the metaplastic and neoplastic transformation of be. nitric oxide (no) has been proposed to be a crucial mediator of barrett's carcinogenesis. we previously demonstrated that cdx2 might be induced directly under stimulation of large amounts of no gene ... | 2016 | 26909236 |
| porphyromonas gingivalis: an overview of periodontopathic pathogen below the gum line. | periodontal disease represents a group of oral inflammatory infections initiated by oral pathogens which exist as a complex biofilms on the tooth surface and cause destruction to tooth supporting tissues. the severity of this disease ranges from mild and reversible inflammation of the gingiva (gingivitis) to chronic destruction of connective tissues, the formation of periodontal pocket and ultimately result in loss of teeth. while human subgingival plaque harbors more than 500 bacterial species, ... | 2016 | 26903954 |
| pyrf as a counterselectable marker for unmarked genetic manipulations in treponema denticola. | the pathophysiology of treponema denticola, an oral pathogen associated with both periodontal and endodontic infections, is poorly understood due to its fastidious growth and recalcitrance to genetic manipulations. counterselectable markers are instrumental in constructing clean and unmarked mutations in bacteria. here, we demonstrate that pyrf, a gene encoding orotidine-5'-monophosphate decarboxylase, can be used as a counterselectable marker in t. denticola to construct marker-free mutants. t. ... | 2016 | 26682856 |
| dysbiosis may trigger autoimmune diseases via inappropriate post-translational modification of host proteins. | the gut ecosystem with myriads of microorganisms and the high concentration of immune system cells can be considered as a separate organ on its own. the balanced interaction between the host and microbial cells has been shaped during the long co-evolutionary process. in dysbiotic conditions, however, this balance is compromised and results in abnormal interaction between the host and microbiota. it is hypothesize here that the changed spectrum of microbial enzymes involved in post-translational ... | 2016 | 26903965 |
| periodontal disease's contribution to alzheimer's disease progression in down syndrome. | people with down syndrome (ds) are at an increased risk for alzheimer's disease (ad). after 60 years of age, >50% of ds subjects acquire dementia. nevertheless, the age of onset is highly variable possibly because of both genetic and environmental factors. genetics cannot be modified, but environmental risk factors present a potentially relevant intervention for ds persons at risk for ad. among them, inflammation, important in ad of ds type, is potential target. consistent with this hypothesis, ... | 2016 | 27239536 |
| long-term oil contamination alters the molecular ecological networks of soil microbial functional genes. | with knowledge on microbial composition and diversity, investigation of within-community interactions is a further step to elucidate microbial ecological functions, such as the biodegradation of hazardous contaminants. in this work, microbial functional molecular ecological networks were studied in both contaminated and uncontaminated soils to determine the possible influences of oil contamination on microbial interactions and potential functions. soil samples were obtained from an oil-exploring ... | 2016 | 26870020 |
| management of peri-implantitis: a systematic review, 2010-2015. | peri-implantitis or periimplantitis is characterized as an inflammatory reaction that affects the hard and soft tissue, which results in loss of supporting bone and pocket formation surrounding the functioning osseointegrated implant. this review aimed to evaluate the effectiveness of surgical and non-surgical treatment of peri-implantitis. the data sources used was pubmed. searches of this database were restricted to english language publications from january 2010 to june 2015. all randomized c ... | 2016 | 26877903 |
| nutrients, microglia aging, and brain aging. | as the life expectancy continues to increase, the cognitive decline associated with alzheimer's disease (ad) becomes a big major issue in the world. after cellular activation upon systemic inflammation, microglia, the resident immune cells in the brain, start to release proinflammatory mediators to trigger neuroinflammation. we have found that chronic systemic inflammatory challenges induce differential age-dependent microglial responses, which are in line with the impairment of learning and mem ... | 2016 | 26941889 |
| interleukin-17a gene variability in patients with type 1 diabetes mellitus and chronic periodontitis: its correlation with il-17 levels and the occurrence of periodontopathic bacteria. | interleukin-17 contributes to the pathogenesis of type 1 diabetes mellitus (t1dm) and chronic periodontitis (cp). we analyzed il-17a -197a/g and il-17f +7488c/t polymorphisms in t1dm and cp and determined their associations with il-17 production and occurrence of periopathogens. totally 154 controls, 125 t1dm, and 244 cp patients were genotyped using 5' nuclease taqman(®) assays. bacterial colonization was investigated by a dna-microarray kit. production of il-17 after in vitro stimulation of mo ... | 2016 | 26924897 |
| comparative genomics reveals new evolutionary and ecological patterns of selenium utilization in bacteria. | selenium (se) is an important micronutrient for many organisms, which is required for the biosynthesis of selenocysteine, selenouridine and se-containing cofactor. several key genes involved in different se utilization traits have been characterized; however, systematic studies on the evolution and ecological niches of se utilization are very limited. here, we analyzed more than 5200 sequenced organisms to examine the occurrence patterns of all se traits in bacteria. a global species map of all ... | 2016 | 26800233 |
| helicobacter pylori colonization of the oral cavity: a milestone discovery. | over the past several years, the severity of helicobacter pylori (h. pylori) infections has not significantly diminished. after successful eradication, the annual h. pylori recurrence rate is approximately 13% due to oral h. pylori infection. established clinical diagnostic techniques do not identify an oral etiologic basis of h. pylori prior to gastric infection. there has been disagreement as to whether oral infection of h. pylori exists or not, with no definite conclusion. in medical practice ... | 2016 | 26811613 |
| a comprehensive benchmarking study of protocols and sequencing platforms for 16s rrna community profiling. | in the last 5 years, the rapid pace of innovations and improvements in sequencing technologies has completely changed the landscape of metagenomic and metagenetic experiments. therefore, it is critical to benchmark the various methodologies for interrogating the composition of microbial communities, so that we can assess their strengths and limitations. the most common phylogenetic marker for microbial community diversity studies is the 16s ribosomal rna gene and in the last 10 years the field h ... | 2016 | 26763898 |
| the rise of crispr/cas for genome editing in stem cells. | genetic manipulation is a powerful tool to establish the causal relationship between a genetic lesion and a particular pathological phenotype. the rise of crispr/cas9 genome-engineering tools overcame the traditional technical bottleneck for routine site-specific genetic manipulation in cells. to create the perfect in vitro cell model, there is significant interest from the stem cell research community to adopt this fast evolving technology. this review addresses this need directly by providing ... | 2016 | 26880991 |
| impact of denture cleaning method and overnight storage condition on denture biofilm mass and composition: a cross-over randomized clinical trial. | appropriate oral hygiene is required to maintain oral health in denture wearers. this study aims to compare the role of denture cleaning methods in combination with overnight storage conditions on biofilm mass and composition on acrylic removable dentures. | 2016 | 26730967 |
| quantitative target analysis and kinetic profiling of acyl-coas reveal the rate-limiting step in cyanobacterial 1-butanol production. | cyanobacterial 1-butanol production is an important model system for direct conversion of co2 to fuels and chemicals. metabolically-engineered cyanobacteria introduced with a heterologous coenzyme a (coa)-dependent pathway modified from clostridium species can convert atmospheric co2 into 1-butanol. efforts to optimize the 1-butanol pathway in synechococcus elongatus pcc 7942 have focused on the improvement of the coa-dependent pathway thus, probing the in vivo metabolic state of the coa-depende ... | 2016 | 26766939 |
| therapeutic targets for management of periodontitis and diabetes. | the increasing incidence of diabetes mellitus (dm) and chronic periodontitis (cp) worldwide imposes a rethinking of individualized therapy for patients with both conditions. central to bidirectional links between dm and cp is deregulated systemic inflammation and dysfunctional immune responses to altered-self and non-self. control of blood glucose levels and metabolic imbalances associated with hyperglycemia in dm, and disruption of pathogenic subgingival biofilms in cp are currently the main th ... | 2016 | 26881443 |
| biofilms as "connectors" for oral and systems medicine: a new opportunity for biomarkers, molecular targets, and bacterial eradication. | oral health and systems medicine are intimately related but have remained, sadly, as isolated knowledge communities for decades. are there veritable connector knowledge domains that can usefully link them together on the critical path to biomarker research and "one health"? in this context, it is noteworthy that bacteria form surface-attached communities on most biological surfaces, including the oral cavity. biofilm-forming bacteria contribute to periodontal diseases and recent evidences point ... | 2016 | 26583256 |
| toll-like receptors in the vascular system: sensing the dangers within. | toll-like receptors (tlrs) are components of the innate immune system that respond to exogenous infectious ligands (pathogen-associated molecular patterns, pamps) and endogenous molecules that are released during host tissue injury/death (damage-associated molecular patterns, damps). interaction of tlrs with their ligands leads to activation of downstream signaling pathways that induce an immune response by producing inflammatory cytokines, type i interferons (ifn), and other inflammatory mediat ... | 2016 | 26721702 |
| in vitro effect of porphyromonas gingivalis methionine gamma lyase on biofilm composition and oral inflammatory response. | methanethiol (methyl mercaptan) is an important contributor to oral malodour and periodontal tissue destruction. porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum are key oral microbial species that produce methanethiol via methionine gamma lyase (mgl) activity. the aim of this study was to compare an mgl knockout strain of p. gingivalis with its wild type using a 10-species biofilm co-culture model with oral keratinocytes and its effect on biofilm composition and infla ... | 2016 | 28033374 |
| assessing the progression of chronic periodontitis using subgingival pathogen levels: a 24-month prospective multicenter cohort study. | the diagnosis of the progression of periodontitis presently depends on the use of clinical symptoms (such as attachment loss) and radiographic imaging. the aim of the multicenter study described here was to evaluate the diagnostic use of the bacterial content of subgingival plaque recovered from the deepest pockets in assessing disease progression in chronic periodontitis patients. | 2017 | 28093069 |
| prevalence of periodontal pathogens among italian patients with chronic periodontitis: a retrospective study on 2992 patients. | the aim of the present study was to evaluate the prevalence of some periodontal pathogens in italian adults with chronic periodontitis. | 2017 | 28757933 |
| the association of periodontopathic bacteria levels in saliva and tongue coating with oral malodor in periodontitis patients. | to elucidate the association oral malodor with the levels of periodontopathic bacteria in saliva and tongue coating of periodontitis patients with oral malodor. | 2017 | 28674708 |
| chronic periodontal disease, periodontal pathogen colonization, and an increased risk of precancerous gastric lesions. | the present study assessed the association between periodontal pathogen colonization and the potential risk of developing precancerous lesions of gastric cancer (plgc) in a clinical setting. | 2017 | 28671506 |
| effects of photodynamic therapy in periodontal treatment: a randomized, controlled clinical trial. | to evaluate the effects of photodynamic therapy (pdt) in the nonsurgical treatment of chronic periodontitis. | 2017 | 28667678 |
| putative periodontal pathogens in the subgingival plaque of sudanese subjects with aggressive periodontitis. | there has been limited study of the bacterial species associated with aggressive periodontitis (agp) in high-risk populations in africa. the aim of this study was to investigate and quantify the presence of four putative periodontal pathogens in the subgingival plaque of sudanese subjects with agp. a secondary aim was to investigate the effect of varying the detection threshold on the reported prevalence of the bacterial species investigated. | 2017 | 28499236 |
| risk of superinfection in peri-implantitis after systemic broad spectrum antibiotics. | peri-implant disease has developed over the last few years as a complication that is often difficult to resolve. the disease process is mainly attributed to bacterial infection. proposed combined therapies use broad-spectrum antibiotics to halt its progression. a major associated risk is the undetected development of superinfections that are difficult to eradicate. a group of healthy individuals with advanced peri-implantitis (pi) were referred for evaluation due to severe, rapidly progressive b ... | 2017 | 28854287 |
| quantification by qpcr of pathobionts in chronic periodontitis: development of predictive models of disease severity at site-specific level. | currently, there is little evidence available on the development of predictive models for the diagnosis or prognosis of chronic periodontitis based on the qpcr quantification of subgingival pathobionts. our objectives were to: (1) analyze and internally validate pathobiont-based models that could be used to distinguish different periodontal conditions at site-specific level within the same patient with chronic periodontitis; (2) develop nomograms derived from predictive models. subgingival plaqu ... | 2017 | 28848499 |
| microbiological response to periodontal therapy and multivariable prediction of clinical outcome. | this study assessed the microbiological effects of a 2-phase antimicrobial periodontal therapy and tested microbiological, clinical, and biological markers as prognostic indicators for clinical success. | 2017 | 28844191 |
| periodontal microbioma and rheumatoid arthritis: the role of porhyromonas gingivalis. | rheumatoid arthritis is a disease, which can be described as an autoimmune response after molecular mimicry caused by infective agents. the current study aims at evaluating the correlation between rhematoid arthritis (ra) and periodontal disease (pd), with special attention to the microbioma detected in the gums. thirty-four patients with rd were recruited into the current study. among rheumatic parameters, rheumatoid factor (rf), anti-citrullinated protein antibody (ccp), hla-bdr1 and das28 wer ... | 2017 | 28691459 |
| simultaneous detection of oral pathogens in subgingival plaque and prostatic fluid of men with periodontal and prostatic diseases. | chronic prostatitis (cpr) and benign prostatic hyperplasia (bph) are complex inflammatory conditions for which etiologic determinants are still poorly defined. periodontitis is caused by subgingival colonizing bacteria in the oral cavity. the causal effect of periodontal disease on prostatic inflammation has not been established. the purpose of this study is to isolate oral pathogens from expressed prostatic secretions of patients with periodontal disease and cpr or bph. | 2017 | 28548883 |
| microbiological and ammp-8 findings depending on peri-implant disease in patients undergoing supportive implant therapy. | the aim of this study was to evaluate microbiological findings and ammp-8 level of peri-implant mucositis (m) and peri-implantitis (p) in patients undergoing supportive implant therapy (sit). eighty-nine patients with 171 implants were included. the case definitions were as follows: m: ppd ≥4mm, bop; p: ppd≥4mm, bop, radiographic bone loss ≥3.5mm. samples of peri-implant sulcular fluid (pisf) were taken from all peri-implant pockets at each implant to detect periodontal pathogens using pcr and a ... | 2017 | 28274501 |
| polymerase chain reaction to evaluate the efficacy of silica dioxide colloidal solutions in the treatment of chronic periodontitis: a case control study. | the objective of this study was to compare the efficacy of supportive periodontal therapy [i.e. scaling and rooth planing (srp)] alone versus a chemical silica dioxide (sio2) colloidal solution (sdcs) device used in association with srp in the treatment of chronic periodontitis in adult patients. a total of 20 patients with a diagnosis of chronic periodontitis (40 localized chronic periodontitis sites) in the age group of 35 to 55 were selected. none of these patients had previously received any ... | 2017 | 26511193 |
| the c- terminal region of the major outer sheath protein (msp) of treponema denticola inhibits neutrophil chemotaxis. | treponema denticola is an oral spirochete strongly associated with severe periodontal disease. a prominent virulence factor, the major outer sheath protein (msp), disorients neutrophil chemotaxis by altering the cellular phosphoinositide balance, leading to impairment of downstream chemotactic events including actin rearrangement, rac1 activation and akt activation in response to chemoattractant stimulation. the specific regions of msp responsible for interactions with neutrophils remain unknown ... | 2017 | 28296262 |
| comparative analysis of motility and other properties of treponema denticola strains. | the periodontitis-associated pathogen treponema denticola is a spirochetal bacterium that swims by rotating its cell body like a corkscrew using periplasmic flagella. we compared physiologic and pathogenic properties, including motility, in four strains of t. denticola. phase-contrast microscopy showed differential motility between the strains; atcc 35404 showed the highest motility, followed by atcc 33521, and the remaining two strains (atcc 35405 and atcc 33520) showed the lowest motility. tra ... | 2017 | 27914958 |
| multilocus sequence analysis of phylogroup 1 and 2 oral treponeme strains. | more than 75 "species-level" phylotypes of spirochete bacteria belonging to the genus treponema reside within the human oral cavity. the majority of these oral treponeme phylotypes correspond to as-yet-uncultivated taxa or strains of uncertain standing in taxonomy. here, we analyze phylogenetic and taxonomic relationships between oral treponeme strains using a multilocus sequence analysis (mlsa) scheme based on the highly conserved 16s rrna, pyrh, reca, and flaa genes. we utilized this mlsa sche ... | 2017 | 27864174 |
| microbial profile comparisons of saliva, pooled and site-specific subgingival samples in periodontitis patients. | the purpose of this study was to compare microbial profiles of saliva, pooled and site-specific subgingival samples in patients with periodontitis. we tested the hypotheses that saliva can be an alternative to pooled subgingival samples, when screening for presence of periopathogens. | 2017 | 28800622 |
| effect of pranayama on ppar-γ, nf-κb expressions and red complex microorganisms in patients with chronic periodontitis - a clinical trial. | sudarshankriya pranayama is the control of breathing movements to regulate the energy flow throughout the body, which has shown to positively affect immune function, autonomic nervous system and psychologic-neuro pathways. the practice of pranayama has been proven to show several benefits such as reduction in stress levels, relieves anxiety and depression, increase in antioxidant levels, reduces insulin resistance and improves respiratory function. | 2017 | 28764300 |
| effect of azithromycin on a red complex polymicrobial biofilm. | azithromycin has recently gained popularity for the treatment of periodontal disease, despite sparse literature supporting efficiency in treating periodontal bacterial biofilms. the aim of this study was to evaluate the effect of azithromycin on biofilms comprised of porphyromonas gingivalis, treponema denticola, and tannerella forsythia in comparison to an amoxicillin and metronidazole combination. p. gingivalis w50, t. denticola atcc35405, and t. forsythia atcc43037 grown under anaerobic condi ... | 2017 | 28748041 |
| periodontal pathogens in the etiology of pancreatic cancer. | pancreatic cancer is the fourth leading cause of cancer-related deaths worldwide. chronic pancreatitis is frequently observed in patients with pancreatic cancer, and a significant relationship between orodigestive cancer-related deaths and chronic periodontitis has been detected. porphyromonas gingivalis, tannerella forsythia, and treponema denticola, collectively called the red complex, are the major pathogens responsible for chronic periodontitis and secrete peptidylarginine deiminase. anti-p. ... | 2017 | 28611978 |
| biomarkers and bacteria around implants and natural teeth in the same individuals. | this cross-sectional study assesses cytokine levels in peri-implant crevicular fluid (picf)/gingival crevicular fluid (gcf) and a selection of subgingival/submucosal plaque bacteria from clinically healthy or diseased sites in the same individuals. | 2017 | 28440740 |
| infection and apoptosis associated with inflammation in periodontitis: an immunohistologic study. | evidence of increased apoptosis is observed in periodontitis and may be associated with destruction of the periodontal tissue caused by the increased cell death, with the release of danger signals and subsequent stimulation of the proinflammatory processes. however, the exact mechanisms associated with these processes remain unclear. this study aimed to investigate the presence of the periodontal pathogen treponema denticola, apoptosis, high mobility group box 1 as a damage-associated molecular ... | 2017 | 28686335 |
| salivary, gingival crevicular fluid and serum levels of ghrelin and chemerin in patients with periodontitis and overweight. | nutrition and body weight are modifying factors for periodontitis. the purpose of this study was to quantify two molecules (ghrelin and chemerin), released in association with food intake and obesity, in periodontally healthy and diseased individuals with respect to different body mass categories. | 2017 | 28631313 |
| effect of surgical periodontal treatment associated to antimicrobial photodynamic therapy on chronic periodontitis: a randomized controlled clinical trial. | this randomized controlled clinical trial evaluated the effects of an adjunctive single application of antimicrobial photodynamic therapy (apdt) in surgical periodontal treatment (st) in patients with severe chronic periodontitis (scp). | 2017 | 28498507 |
| oral treponeme major surface protein: sequence diversity and distributions within periodontal niches. | treponema denticola and other species (phylotypes) of oral spirochetes are widely considered to play important etiological roles in periodontitis and other oral infections. the major surface protein (msp) of t. denticola is directly implicated in several pathological mechanisms. here, we have analyzed msp sequence diversity across 68 strains of oral phylogroup 1 and 2 treponemes; including reference strains of t. denticola, treponema putidum, treponema medium, 'treponema vincentii', and 'trepone ... | 2017 | 28453906 |
| prevalence of eight putative periodontal pathogens in atherosclerotic plaque of coronary artery disease patients and comparing them with noncardiac subjects: a case-control study. | the study was aimed to assess the prevalence of periodontal pathogens namely tannerella forsythia (t.f), campylobacter rectus (c.r), eikenella corrodens (e.c), porphyromonas gingivalis (p.g), treponema denticola (t.d), prevotella nigrescens (p.n) ,aggregatibacter actinomycetemcomitans (a.a), p.g (fi ma gene) and prevotella intermedia (p.i), in the subgingival and the atheromatous plaque of patients with coronary artery disease (cad), and comparing them with the noncardiac subjects thereby indica ... | 2017 | 26096116 |
| evaluation of a hydrophobic gel adhering to the gingiva in comparison with a standard water-soluble 1% chlorhexidine gel after scaling and root planing in patients with moderate chronic periodontitis. a randomized clinical trial. | to evaluate the clinical, microbiological and enzymatic activity of a hydrophobic chlorhexidine-based gingiva-adhering gel containing herbal ingredients, compared with a commercially available 1% chlorhexidine water-soluble gel, during non-surgical therapy of moderate chronic periodontitis. | 2017 | 26053503 |
| a double-blind randomized trial comparing implants with laser-microtextured and machined collar surfaces: microbiologic and clinical results. | the purpose of this study was to compare the clinical outcome of and determine the differences in periodonto-pathogenic microbiota around two types of implant collar surfaces: laser-microtextured (test) vs machined (control). | 2017 | 27632268 |
| comparison of two different sampling methods for subgingival plaque: subgingival paper points or mouthrinse sample? | the collection of subgingival plaque samples with paper points is time-consuming and accident-sensitive. however, the collection of saliva is simple and contains pathogens of all intraoral surfaces. the aim of this study is to investigate whether a sampling strategy with mouthrinse (mouthrinse sample [msp]; test) leads to results comparable with standard sampling method (multiple site test from the deepest pocket of each quadrant [mt4]; control). | 2017 | 27786617 |
| reproducibility of a commercially available subgingival plaque sampling strategy and analysis strategy with oligonucleotide probes. | the aim was to evaluate the intra-test agreement of pooled samples from the deepest periodontal pocket of each quadrant with a commercially available test kit based on hybridization of 16s rrna. | 2017 | 28325127 |
| effects of periodontal treatment on primary sjȫgren's syndrome symptoms. | the aim of this longitudinal prospective study was to evaluate the effects of periodontal treatment on the clinical, microbiological and immunological periodontal parameters, and on the systemic activity (essdai) and subjective (esspri) indexes in patients with primary sjögren's syndrome (pss). twenty-eight female patients were divided into four groups: pss patients with or without chronic periodontitis (scp, sc, respectively), and systemically healthy patients with or without chronic periodonti ... | 2017 | 28099577 |
| secretable small rnas via outer membrane vesicles in periodontal pathogens. | micrornas (mirnas) have been shown to be major regulators of eukaryotic gene expression. however, bacterial rnas comparable in size to eukaryotic mirnas (18-22 nucleotides) have received little attention. recently, a novel class of small rnas similar in size to mirnas (mirna-size, small rnas or msrnas) have also been found in several bacteria. like mirnas, msrnas are approximately 15 to 25 nucleotides in length, and their precursors are predicted to form a hairpin loop secondary structure. here, ... | 2017 | 28068479 |
| high-risk periodontal pathogens contribute to the pathogenesis of atherosclerosis. | periodontal disease (pd) is generated by microorganisms. these microbes can enter the general circulation causing a bacteraemia. the result can be adverse systemic effects, which could promote conditions such as cardiovascular disease. level a evidence supports that pd is independently associated with arterial disease. pd is a common chronic condition affecting the majority of americans 30 years of age and older. atherosclerosis remains the largest cause of death and disability. studies indicate ... | 2017 | 27899684 |
| periodontal bacteria in the genital tract: are they related to adverse pregnancy outcome? | one of the most important factors implicated in preterm birth (ptb) is acute genitourinary tract infection. the bacteria causing chronic periodontal inflammation include gram-negative rods and anaerobes similar to those found in women with bacterial vaginosis. the aim of this prospective study is to investigate the relationship between oral and vaginal microflora and preterm low birth weight. real-time polymerase chain reaction was used to detect both the presence and level of six periodontitis- ... | 2017 | 24355228 |
| persistence of porphyromonas gingivalis is a negative predictor in patients with moderate to severe periodontitis after nonsurgical periodontal therapy. | the aim of this study was to evaluate the quality of prediction for stable results after nonsurgical periodontal therapy by several microbiological variables of the subgingival biofilm and biomarkers of gingival crevicular fluid or oral lavage. | 2017 | 27558382 |
| treponema denticola, porphyromonas gingivalis, and tannerella forsythia induce cell death and release of endogenous danger signals. | the aim of this study was to analyze whether periodontopathogens induced inflammatory cell death and the release of diverse endogenous danger molecules in thp-1-derived macrophages. | 2017 | 27697692 |
| [the relationship of molecular genetic markers with clinical signs and risk factors of periodontitis]. | the study revealed positive correlation between bleeding on probing and teeth loss risk with periodontal hypercolonization by porphyromonas gingivalis, prevotella intermedia and treponema denticola. pathological tooth mobility was associated with hypercolonization by p. intermedia and tannerella forsythensis. expression of il8, tnf-α, mmp8 and mmp9 genes was also assessed in patient groups divided according to the depth of periodontal pockets and-the severity of chronic periodontitis revealing i ... | 2017 | 27876716 |
| a novel rat model of polymicrobial peri-implantitis: a preliminary study. | peri-implantitis is a complex polymicrobial biofilm-induced inflammatory osteolytic gingival infection that results in orofacial implant failures. to the best knowledge of the authors, there are no preclinical in vivo studies in implant dentistry that have investigated the inflammatory response to known microbial biofilms observed in humans. the aim of this study is to develop a novel peri-implant rat model using an established model of polymicrobial periodontitis. | 2017 | 27786620 |
| the risk reduction of recurrent periodontal pathogens of local application minocycline hcl 2% gel, used as an adjunct to scaling and root planing for chronic periodontitis treatment. | the aim of this study was to evaluate the clinical and microbiological effects of local application minocycline hcl 2% gel, used as an adjunct to scaling and root planing (srp) for treatment of chronic periodontitis (cp). cp is an inflammation of periodontal tissue that is caused mainly by bacterial infection, where periodontal destruction such as loss of attachment and bone destruction occurred. | 2017 | 28331333 |
| clinical and microbiological efficacy of 4% garcinia mangostana l. pericarp gel as local drug delivery in the treatment of chronic periodontitis: a randomized, controlled clinical trial. | the aim of the present study was to assess the periodontal status of individuals and the presence of red complex microorganisms (rcm), such as treponema denticola (td), porphyromonas gingivalis, and tannerella forsythia in the subgingival tissues of periodontitis patients before and after the application of 4% mangostana gel (garcinia mangostana [mga]) as an adjunct to scaling and root planing (srp). | 2017 | 28321995 |
| "association of periodontopathic anaerobic bacterial co-occurrence to atherosclerosis" - a cross-sectional study. | epidemiological studies have shown a link between periodontitis and atherosclerosis. hence the present study was chosen to assess the presence of eight anaerobic periodontal pathogens and their virulence genes in subgingival plaque (sgp) and atheromatous plaque (ap) of patients who underwent coronary artery bypass graft surgery (cabg). | 2017 | 28189829 |
| molecular-level evaluation of selected periodontal pathogens from subgingival regions in canines and humans with periodontal disease. | dogs commonly serve as a model for various human conditions, including periodontal diseases. the aim of this study was to identify the anaerobic bacteria that colonize the subgingival areas in dogs and humans by using rapid real-time polymerase chain reaction (rt-pcr)-based tests and to compare the results obtained in each species. bacterial microflora evaluations, both quantitative and qualitative, were performed by applying ready-made tests on twelve dogs and twelve humans. five samples were c ... | 2017 | 27297417 |
| evaluation of bacteriological profile in the apical root segment of the patients with primary apical periodontitis. | apical periodontitis usually results from bacterial accumulation and contamination occurring in the root-canal system, and extending beyond the apical foramen to involve the periapical tissues. literature has a paucity of the studies that stress on the division and analysis of the pulp canal segments. the reason for this disparity might be the technique used for collecting the samples from the pulp canals. hence, we carried out the present study to evaluate the microbial flora in the apical part ... | 2017 | 28050984 |
| influence of glycemic control on the levels of subgingival periodontal pathogens in patients with generalized chronic periodontitis and type 2 diabetes. | this study evaluated the influence of glycemic control on the levels and frequency of subgingival periodontal pathogens in patients with type 2 diabetes mellitus (dm) and generalized chronic periodontitis (chp). | 2017 | 28198980 |
| microbial colonization at the implant-abutment interface and its possible influence on periimplantitis: a systematic review and meta-analysis. | the aim of this systematic review and meta-analysis was to evaluate the microbial colonization at the implant-abutment interfaces (iai) on bone-level implants and to identify possible association with peri-implant conditions. | 2017 | 28359872 |
| sequential colonization of periodontal pathogens in induction of periodontal disease and atherosclerosis in ldlrnull mice. | periodontal disease (pd) and atherosclerotic vascular disease (asvd) are both chronic inflammatory diseases with a polymicrobial etiology and have been epidemiologically associated. the purpose is to examine whether periodontal bacteria that infect the periodontium can also infect vascular tissues and enhance pre-existing early aortic atherosclerotic lesions in ldlrnull mice. mice were orally infected with intermediate bacterial colonizer fusobacterium nucleatum for the first 12 weeks followed b ... | 2017 | 28104616 |
| cross-sectional evaluation of periodontal status and microbiologic and rheumatoid parameters in a large cohort of patients with rheumatoid arthritis. | this study evaluates periodontal conditions and microbiologic findings and their influence on rheumatologic disease parameters in patients with rheumatoid arthritis (ra). | 2017 | 27858553 |
| a novel glycan modifies the flagellar filament proteins of the oral bacterium treponema denticola. | while protein glycosylation has been reported in several spirochetes including the syphilis bacterium treponema pallidum and lyme disease pathogen borrelia burgdorferi, the pertinent glycan structures and their roles remain uncharacterized. herein, a novel glycan with an unusual chemical composition and structure in the oral spirochete treponema denticola, a keystone pathogen of periodontitis was reported. the identified glycan of mass 450.2 da is composed of a monoacetylated nonulosonic acid (n ... | 2017 | 27696564 |
| quantitative analysis of periodontal pathogens in periodontitis and gingivitis. | periodontal tissues surround the teeth and provide their attachment. periodontal diseases include a mild and reversible form named gingivitis and periodontitis that is the main cause of tooth loss in adults. gingivitis, that affects gums and coronal junctional epithelium, as well as periodontitis, that is characterized by loss of connective tissue attachment, are caused by a persistent inflammatory response promoted by alteration of periodontal biofilm. the aim of the study was to test whether t ... | 2017 | 26511188 |
| the infectious etiology of alzheimer's disease. | inflammation is a part of the first line of defense of the body against invasive pathogens, and plays a crucial role in tissue regeneration and repair. a proper inflammatory response ensures the suitable resolution of inflammation and elimination of harmful stimuli, but when the inflammatory reactions are inappropriate it can lead to damage of the surrounding normal cells. the relationship between infections and alzheimer's disease (ad) etiology, especially late-onset ad (load) has been continuo ... | 2017 | 28294067 |
| 1,2-diacylglycerol choline phosphotransferase catalyzes the final step in the unique treponema denticola phosphatidylcholine biosynthesis pathway. | treponema denticola synthesizes phosphatidylcholine through a licca-dependent cdp-choline pathway identified only in the genus treponema. however, the mechanism of conversion of cdp-choline to phosphatidylcholine remained unclear. we report here characterization of tde0021 (herein designated cpt) encoding a 1,2-diacylglycerol choline phosphotransferase homologous to choline phosphotransferases that catalyze the final step of the highly conserved kennedy pathway for phosphatidylcholine synthesis ... | 2017 | 28009086 |
| butyrate production under aerobic growth conditions by engineered escherichia coli. | butyrate is an important industrial platform chemical. although several groups have reported butyrate production under oxygen-limited conditions by a native producer, clostridium tyrobutylicum, and by a metabolically engineered escherichia coli, efforts to produce butyrate under aerobic growth conditions have met limited success. here, we constructed a novel butyrate synthetic pathway that functions under aerobic growth conditions in e. coli, by modifying the 1-butanol synthetic pathway reported ... | 2017 | 28089378 |
| analysis of the expression of nlrp3 and aim2 in periapical lesions with apical periodontitis and microbial analysis outside the apical segment of teeth. | to detect the distribution and expression levels of the nucleotide-binding oligomerization domain-like receptor protein 3 (nlrp3) and the absent in melanoma 2 (aim2) inflammasomes in periapical lesions and to analyse the possible microbial stimuli outside of teeth. | 2017 | 28193569 |