Publications

simultaneous determination of unbound thyroid hormones in human plasma using high performance frontal analysis with electrochemical detection.	a direct injection hplc method in combination with high-performance frontal analysis (hpfa) and electrochemical detection (ecd) was developed for the simultaneous and sensitive determination of unbound thyroid hormones (thyroxine, triiodothyronine, and reverse triiodothyronine) in human plasma. the present on-line hplc/hpfa system consists of an hpfa column, an extraction column and an analytical hplc column connected through a column-switching device, and the eluent from the analytical column w ...	2005	15925209
impact of intensity-modulated radiation therapy as a boost treatment on the lung-dose distributions for non-small-cell lung cancer.	to investigate the feasibility of intensity-modulated radiotherapy (imrt) as a method of boost radiotherapy after the initial irradiation by the conventional anterior/posterior opposed beams for centrally located non-small-cell lung cancer through the evaluation of dose distributions according to the various boost methods.	2005	15927412
electrokinetic transport of rigid macroions in the thin double layer limit: a boundary element approach.	a boundary element (be) procedure is developed to numerically calculate the electrophoretic mobility of highly charged, rigid model macroions in the thin double layer regime based on the continuum primitive model. the procedure is based on that of o'brien (r.w. o'brien, j. colloid interface sci. 92 (1983) 204). the advantage of the present procedure over existing be methodologies that are applicable to rigid model macroions in general (s. allison, macromolecules 29 (1996) 7391) is that computati ...	2005	15927633
multimodality treatment of esophageal cancer.	stage specific management of non-small cell lung cancer is widely accepted. the use of pretreatment disease stage to guide therapy for esophageal cancer is an intellectually appealing concept. to date, there isa relative lack of data upon which one may base stage specific treatment decisions for esophageal carcinoma. this is because thorough pretreatment tnm staging is not universally practiced. as a result, stage-specific treatment varies widely. based upon the available data, surgery alone may ...	2005	15927656
ovarian iodide uptake and triiodothyronine generation in follicular fluid. the enigma of the thyroid ovary interaction.	since 1928, the iodine concentration in the ovary has been known to be higher than in every other organs except the thyroid. the ovarian iodide uptake varies with sexual activities, is enhanced by estrogens and a hypothyroid state and blocked by goitrogens. the recent discovery of a sodium iodide symporter (nis) in ovaries has offered a possible mechanism for ovarian iodide uptake and other functional similarities to its thyroid counterpart. nevertheless, the physiological significance of ovaria ...	2005	15927769
review: treatment of hypothyroidism with combinations of levothyroxine plus liothyronine.	combined infusion of levothyroxine plus liothyronine, as opposed to levothyroxine alone, is the only way of restoring the concentrations of circulating tsh, t4, and t3 as well as those of both t4 and t3 in all tissues of thyroidectomized rats. considering the substantial differences in thyroid hormone secretion, transport, and metabolism between rats and humans, whether or not combined levothyroxine plus liothyronine replacement therapy has advantages over treatment with levothyroxine alone in h ...	2005	15928247
bisphenol-a, an environmental contaminant that acts as a thyroid hormone receptor antagonist in vitro, increases serum thyroxine, and alters rc3/neurogranin expression in the developing rat brain.	considering the importance of thyroid hormone (th) in brain development, it is of potential concern that a wide variety of environmental chemicals can interfere with thyroid function or, perhaps of greater concern, with th action at its receptor (tr). recently bisphenol-a (bpa, 4,4' isopropylidenediphenol) was reported to bind to the rat tr and act as an antagonist in vitro. bpa is a high production volume chemical, with more than 800 million kg of bpa produced annually in the united states alon ...	2005	15498886
reevaluating thyrotropin receptor-induced mouse models of graves' disease and ophthalmopathy.	we aimed to establish and extend the characterization of murine models of thyroiditis and graves' ophthalmopathy, induced by transfer of tsh receptor (tshr) primed t cells. experiments were performed in a different animal unit but using female balb/cbyjico mice from the same supplier as previously. we report our findings together with a reevaluation of the earlier studies. in the first experiment, genetic immunization or tshr fusion protein induced tshr antibodies in all nine mice. some of the a ...	2005	15542454
mechanism of chaperone function in small heat shock proteins: dissociation of the hsp27 oligomer is required for recognition and binding of destabilized t4 lysozyme.	mammalian small heat shock proteins (shsp) form polydisperse and dynamic oligomers that undergo equilibrium subunit exchange. current models of their chaperone activity hypothesize that recognition and binding of protein non-native states involve changes in the oligomeric state. the equivalent thermodynamic representation is a set of three coupled equilibria that includes the shsp oligomeric equilibrium, the substrate folding equilibrium, and the equilibrium binding between the shsp and the subs ...	2005	15542604
32p-postlabeling analysis of dna adducts.	32p-postlabeling analysis is an ultrasensitive method for the detection of dna adducts, such as those formed directly by the covalent binding of carcinogens and mutagens to bases in dna, as well as other dna lesions resulting from modification of bases by endogenous or exogenous agents (e.g., oxidative damage). the procedure involves four main steps: enzymatic digestion of a dna sample; enrichment of the adducts; radiolabeling of the adducts by t4 kinase-catalyzed transference of 32p-orthophosph ...	2005	15502206
modification of the 32p-postlabeling method to detect a single adduct species as a single spot.	the original 32p-postlabeling method developed by randerath and his colleagues has been modified to detect a single type of adduct as a single spot in thin-layer chromatography (tlc), because some types of adducts gave multiple adduct spots by the original method. in the remodified methods, dna is first digested with micrococcal nuclease and phophodiesterase ii and then labeled with [gamma-32p]atp under standard or adduct-intensification conditions. since the labeled digest includes adducted mon ...	2005	15502207
the role of an activating peptide in protease-mediated suicide of escherichia coli k12.	activation of latent proteinases ensures that the timing of proteolysis is regulated precisely, a process that generally involves proteolytic excision of a pro-region or a tightly bound inhibitor. here we define the activation mechanism for lit, a dormant suicide proteinase in escherichia coli k-12. previous work has shown that gol, a short sequence within the major capsid protein gp23, activates lit during the latter stages of t4 phage infection. this results in cell death and exclusion of the ...	2005	15501819
quantitative trait loci associated with elevated thyroid-stimulating hormone in the wistar-kyoto rat.	thyroid hormones are essential for the regulation of developmental and physiological processes. the genetic factors underlying naturally occurring variability in mammalian thyroid function are, however, only partially understood. genetic control of thyroid function can be studied with animal models such as the inbred wistar-kyoto (wky) rat strain. previous studies established that wky rats have elevated tsh, slightly elevated total t3, and normal total t4 levels compared with wistar controls. th ...	2005	15514085
a muc1 tandem repeat reporter protein produced in cho-k1 cells has sialylated core 1 o-glycans and becomes more densely glycosylated if coexpressed with polypeptide-galnac-t4 transferase.	a recombinant mucin o-glycosylation reporter protein, containing 1.7 tandem repeats (trs) from the transmembrane mucin muc1, was constructed. the reporter protein, muc1(1.7tr)-igg2a, was produced in cho-k1 cells to study the glycosylation of the muc1 tr and the in vivo role of polypeptide-galnac-t4 glycosyltransferase. n-terminal sequencing of muc1(1.7tr)-igg2a showed that all five potential o-glycosylation sites within the tr were used, with an average density of 4.5 glycans per repeat. the lea ...	2005	15456735
type 2 iodothyronine selenodeiodinase is expressed throughout the mouse skeleton and in the mc3t3-e1 mouse osteoblastic cell line during differentiation.	thyroid hormone affects multiple aspects of bone metabolism, but little is known about thyroid hormone deiodination in bone cells except that cultures of skeletal cells and bone organ express types 1 and 2 iodothyronine deiodinases (d1 and d2) mrnas. in the present study, outer ring deiodination (ord) activity was detected in bone extracts of multiple sites of the mouse skeleton, bone marrow, and the mc3t3-e1 osteoblastic cell line. in all tissues, ord was detected using 125i-rt3 or 125i-t4 as s ...	2005	15471965
the role of type i and type ii 5' deiodinases on hexachlorobenzene-induced alteration of the hormonal thyroid status.	treatment of male wistar rats with hexachlorobenzene (hcb) (1000 mg/kg b.w.) for 3-30 days decreases circulating levels of thyroxine (t4) but does not affect triiodothyronine (t3). time courses were determined for 5' deiodinase type i (5' d-i) activity in thyroid, liver, and kidney and 5' deiodinase type ii (5' d-ii) activity in brown adipose tissue (bat) to test the possibility that increased deiodinase activity might contribute to the maintenance of the serum t3 level. specific 5' d-i activity ...	2005	15664263
hyperthyroidism changes nociceptive response and ecto-nucleotidase activities in synaptosomes from spinal cord of rats in different phases of development.	changes in transport, receptors and production of extracellular adenosine have been observed after induction of hyperthyroidism. adenosine is associated with inhibitory actions such as reduction in release of excitatory neurotransmitters and antinociception at spinal site. in contrast, atp acts as an excitatory neurotransmitter and produces pronociceptive actions. atp may be completely hydrolyzed to adenosine by an enzyme chain constituted by an atp diphosphohydrolase and an ecto-5'-nucleotidase ...	2005	15664319
effects of butylated hydroxyanisole on the development and functions of reproductive system in rats.	butylated hydroxyanisol (bha) is a widely used antioxidant for long preservation of food products, cosmetics and pharmaceuticals. although bha is generally recognized as safe, it is classified as a suspected endocrine-disrupting compound. we investigated the effects of bha on reproductive function and development by the treatment of mature male and female sd rats (f0) through pre-gestation, gestation and lactation period and of their offspring (f1) until 13 weeks old via gavage with bha 0 (corn ...	2005	15664432
molecular mechanisms of high level tetracycline-resistance in group a streptococcal isolates, t serotypes 4 and 11.	the molecular mechanism of high level tetracycline resistance in t serotypes 4 and 11 group a streptococcal (gas) isolates was examined in 61 tetracycline-resistant isolates in japan. pcr and sequencing analyses revealed that the t serotype/emm genotype, t4/4 isolates carried tet(o) genes, which were genetically homogenous. the t11/11 and t11/89 isolates carried different subtypes of tet(m) genes, which were present on transposons tn916 and tn1545, respectively. in addition, these t11 isolates m ...	2005	15664484
thyroid hormone transporters.	thyroid hormone is important for development of various tissues, in particular brain, and for regulation of metabolic processes throughout life. the follicular cells of the thyroid gland produce predominantly t4 (thyroxine), but the biological activity of thyroid hormone is largely exerted by t3 (3,3',5-tri-iodothyronine). the deiodinases involved in t4-to-t3 conversion or t4 and t3 degradation, as well as the t3 receptors, are located intracellularly. therefore the action and metabolism of thyr ...	2005	15667314
effect of nicardipine combined with esmolol on systemic and tissue oxygenation during off-pump coronary artery bypass grafting surgery.	the hemodynamics and oxygenation severely fluctuated during the off-pump coronary artery bypass grafting (opcabg). this study aimed at investigating whether or not nicardipine combined with esmolol (1:10) can maintain systemic and tissue oxygenation during opcabg.	2005	15667798
comparison of late rectal toxicity from conventional versus three-dimensional conformal radiotherapy for prostate cancer: analysis of clinical and dosimetric factors.	to compare late rectal toxicity (lrt) after definitive radiotherapy (dr) and salvage radiotherapy (sr) in prostate cancer using conventional (conv) or three-dimensional conformal (3-d) techniques.	2005	15667875
growth of dalbergia sissoo in desert regions of western india using municipal effluent and the subsequent changes in soil and plant chemistry.	increasing demand for fodder and fuelwood and the scarcity of a good quality water in arid areas has resulted in a search for an alternative source of water for biomass production. an experiment utilizing municipal effluent in growing dalbergia sissoo was conducted. five treatments included t1, municipal effluent at 1 pet (potential evapo-transpiration) (without plant); t2, municipal effluent at 1/2 pet; t3, municipal effluent at 1pet; t4, municipal effluent at 2 pet; and t5, canal water at 1 pe ...	2005	15668198
myocardial ultrastructure in cardiac hypertrophy induced by thyroid hormone--an acute study in rats.	the early responses of the myocardium ultrastructure under thyroid dysfunction conditions, hemodynamic parameters, cardiac hypertrophy and ultrastructural evaluations were performed in hypothyroid and hyperthyroid rats submitted to different doses [t4-25 and t4-100; 0.025 mg and 0.1 mg kg(-1) body weight (bw).per day, respectively)]. all groups were treated for 7 days. the animals were sacrificed, the hearts were excised and weighed and the left ventricle tissue samples were processed for transm ...	2005	15668802
laryngeal cancer patients: analysis of patient delay at different tumor stages.	the aim of this study was to determine the length of stages (appraisal, illness, behavioral, and scheduling) of patient delay in patients with head and neck cancer and to find out whether these delays were related to the stage of the disease at diagnosis.	2005	15668927
is stimulation of thyroglobulin (tg) useful in low-risk patients with thyroid carcinoma and undetectable tg on thyroxin and negative neck ultrasound?	to determine the usefulness of thyroglobulin (tg) stimulation in low-risk patients with undetectable tg on t4 and negative neck ultrasound (us) after initial therapy of thyroid carcinoma.	2005	15670185
changes in thyroid hormone state in children receiving chemotherapy.	the concentrations of thyroid function determinants may change during severe illness. our goal was to quantify their changes in children with cancer during chemotherapy, and to correlate them to clinical condition and type of drugs.	2005	15670204
alternative input devices for efficient navigation of large ct angiography data sets.	to compare devices for the task of navigating through large computed tomographic (ct) data sets at a picture archiving and communication system workstation.	2005	15670996
colocalization of carbonic anhydrase 9 expression and cell proliferation in human head and neck squamous cell carcinoma.	tumor cells undergo a variety of biological changes under sustained hypoxic conditions, allowing cells to survive and retain their clonogenic potential. the purpose of this study is to relate the expression of the hypoxia marker carbonic anhydrase 9 (ca9) to the uptake of iododeoxyuridine (idurd), a marker of proliferation, in head and neck squamous cell carcinomas. colocalization of idurd and ca9 may identify an important subpopulation of tumor cells that might be responsible for repopulation a ...	2005	15671533
chemically modified dna substrates implicate the importance of electrostatic interactions for dna unwinding by dda helicase.	helicase-catalyzed disruption of double-stranded nucleic acid is vital to dna replication, recombination, and repair in all forms of life. the relative influence of specific chemical interactions between helicase and the substrate over a series of multistep catalytic events is still being defined. to this end, three modified dna oligonucleotides were designed to serve as substrates for the bacteriophage t4 helicase, dda. a 5'-dna-pna-dna-3' chimera was synthesized, thereby, conferring both a los ...	2005	15641792
[clinical analysis of 123 cases of transitional cell carcinoma (tcc) of upper urinary tract].	with the development of diagnostic techniques of imaging, and application of endoscope, early diagnosis and treatment of transitional cell carcinoma (tcc) of upper urinary tract have been improved to a great extent in recent years, but still caused debates. in this article, we discussed the diagnostic and therapeutic methods of tcc of upper urinary tract.	2005	15642209
isolation and characterization of a thermostable rna ligase 1 from a thermus scotoductus bacteriophage ts2126 with good single-stranded dna ligation properties.	we have recently sequenced the genome of a novel thermophilic bacteriophage designated as ts2126 that infects the thermophilic eubacterium thermus scotoductus. one of the annotated open reading frames (orfs) shows homology to t4 rna ligase 1, an enzyme of great importance in molecular biology, owing to its ability to ligate single-stranded nucleic acids. the orf was cloned, and recombinant protein was expressed, purified and characterized. the recombinant enzyme ligates single-stranded nucleic a ...	2005	15642699
her-2/neu and p53 overexpression as biomarkers of breast carcinoma in women age 30 years and younger.	although p53 and her-2/neu overexpression are found in 30% of breast carcinomas in women of all ages and are associated with high rates of lymph node metastases, their role in women age < 30 years has not been studied extensively.	2005	15643600
effects of chronic inhibition of inducible nitric oxide synthase in hyperthyroid rats.	we hypothesized that nitric oxide generated by inducible nitric oxide synthase (inos) may contribute to the homeostatic role of this agent in hyperthyroidism and may, therefore, participate in long-term control of blood pressure (bp). the effects of chronic inos inhibition by oral aminoguanidine (ag) administration on bp and morphological and renal variables in hyperthyroid rats were analyzed. the following four groups (n = 8 each) of male wistar rats were used: control group and groups treated ...	2005	15644461
oral squamous cell carcinoma: histologic risk assessment, but not margin status, is strongly predictive of local disease-free and overall survival.	to analyze the impact of resection margin status and histologic prognosticators on local recurrence (lr) and overall survival (os) for patients with oral squamous cell carcinoma (oscc). this study was both retrospective and prospective in design. cohort 1 refers to the entire group of 292 patients with oscc. the slides from the earliest resection specimens from cohort 1 were examined in an exploratory manner for multiple parameters. cohort 2 refers to a subset of 203 patients, who did not receiv ...	2005	15644773
quantification of antimalarial bisthiazolium compounds and their neutral bioprecursors in plasma by liquid chromatography-electrospray mass spectrometry.	a new class of antimalarial drugs targeting membrane biogenesis during intraerythrocytic plasmodium falciparum development has been identified. the bisthiazolium salts t3 and t4 have superior in vitro and in vivo parasite-killing properties and need to be monitored.	2005	15637133
chronic estradiol exposure modulates thyroid structure and decreases t4 and t3 serum levels in middle-aged female rats.	in human medicine, estrogen is applied in prevention and treatment of health problems associated with the menopause. the aim of this study was to examine the effects of chronic estradiol dipropionate (edp) treatment on thyroid gland structure and function in middle-aged female rats.	2005	15637454
time-resolved fluorescence of the bacteriophage t4 capsid protein gp23.	the time-resolved fluorescence properties of the bacteriophage t4 capsid protein gp23 are investigated. the structural characteristics of this protein are largely unknown and can be probed by recording time-resolved and decay-associated fluorescence spectra and intensity decay curves using a 200 ps-gated intensified ccd-camera. spectral and decay data are recorded simultaneously, which makes data acquisition fast compared to time-correlated single-photon counting. a red-shift of the emission max ...	2005	15629249
symptom-free t4 lung cancer with bilateral pulmonary artery infiltration.		2005	15629279
clinical results of proton beam therapy for cancer of the esophagus.	to present the results of proton beam therapy for patients with esophageal cancer.	2005	15629597
long-term results of adjuvant hypofractionated radiotherapy for breast cancer in elderly patients.	to evaluate early and late reactions, local control, disease-free survival, cause-specific survival, and overall survival of elderly breast cancer patients treated with adjuvant once-weekly hypofractionated radiotherapy (rt).	2005	15629606
gastrostomy tubes in patients with advanced head and neck cancer.	to measure the percentage of patients requiring gastrostomy tubes (g-tubes) and the timing of their placement, in addition to studying whether pretreatment variables (t stage, tumor site, n stage) and intratreatment variables (weight loss during treatment) are valid predictors for the need for g-tube placement.	2005	15630364
histopathologic validation of the sentinel node concept in oral and oropharyngeal squamous cell carcinoma.	in patients with head and neck squamous cell carcinoma (hnscc), the presence of lymph node metastases is the most important prognosticator. sentinel node (sn) biopsy has been shown to be an accurate staging technique for patients with breast cancer and melanoma and might also be suited for patients with hnscc. this study was undertaken to determine whether the sn concept holds true for hnscc and could be exploited for sn biopsy.	2005	15627261
accuracy of magnetic resonance imaging in predicting absence of fixation of head and neck cancer to the prevertebral space.	the purpose of this study was to determine the preoperative accuracy of preservation of the retropharyngeal fat plane on magnetic resonance (mr) images in predicting the absence of fixation or extension of head and neck carcinomas to the prevertebral space.	2005	15627263
regulation of cerebellar neuronal migration and neurite outgrowth by thyroxine and 3,3',5'-triiodothyronine.	the timing of granule cell migration in the developing cerebellum is regulated by thyroid hormone. granule cell migration depends on the recognition of extracellular neuronal guidance molecule(s), such as laminin, and this, in turn, requires cell surface adhesion molecules (integrins) that are anchored on the cell membrane by the actin cytoskeleton. while many of the actions of thyroid hormone, specifically 3,5,3'-triiodothyronine (t3), are mediated by regulated gene expression, both thyroxine ( ...	2005	15617761
single acceleromyographic train-of-four, 100-hertz tetanus or double-burst stimulation: which test performs better to detect residual paralysis?	acceleromyography is regularly used as an isolated test to detect residual paralysis. the performance of acceleromyography, however, has not been investigated for the setting where calibration is impossible. this study first evaluated the reliability of a single acceleromyographic train-of-four (tof) ratio (t4/t1) to detect residual paralysis and compared it with tactile estimation of fade after double-burst stimulation and 100-hz, 5-s tetanus. the second part of the study investigated whether u ...	2005	15618786
extended pneumonectomy with partial resection of the left atrium, without cardiopulmonary bypass, for lung cancer.	extended pneumonectomy with partial resection of the left atrium for lung cancer is not frequently performed; therefore, its results remain controversial. the present study analyzed a single center's experience with this extended surgery, highlighting the surgery's technical aspects, postoperative outcomes, and oncologic results.	2005	15620949
clinical evaluation of systemic inflammatory response syndrome (sirs) in advanced lung cancer (t3 and t4) with surgical resection.	the systemic inflammatory response syndrome (sirs) is well known to occur in patients who have suffered organ damage or trauma, or undergone surgery. sirs provides useful information in patients with morbidity after surgery. to date, there has been no report of sirs after surgery in patients with lung cancer. therefore, based on this new concept of the syndrome, we review here a series of t3 and t4 nsclc patients who underwent extended resection at our hospital, and attempt to identify the value ...	2005	15621464
outcome following surgery for primary lung cancer with interlobar pleural invasion.	to determine whether interlobar pleural invasion into the adjacent lobe (interlobar p3) should be assessed as t3 according to the tumor-node metastasis classification.	2005	15622459
relationship between anxiety and thyroid function in patients with panic disorder.	the aim of this study was to investigate correlations between thyroid function and severity of anxiety or panic attacks in patients with panic disorder. the authors examined 66 out-patients with panic disorder (medicated, n=41; non-medicated, n=25), and measured their free thriiodothyronine (t3), free thyroxine (t4) and thyroid-stimulating hormone (tsh) levels. significant correlations between the thyroid hormone levels and clinical features were observed in the non-medicated patients. the more ...	2005	15610948
mucoepidermoid carcinoma of the salivary glands--clinical review of 42 cases.	in this retrospective study we analyzed our experience with mucoepidermoid carcinoma (mec) of the salivary glands and compared our results with those reported in the literature. a total of 42 patients were treated between january 1983 and december 2002 at the department of oral and maxillofacial surgery, hanover medical school. thirty-three tumors were classified as low grade carcinomas, 9 tumors as high grade carcinomas. tumor sites were the parotid gland in 11 cases, sublingual and submandibul ...	2005	15598579
impact of egfr expression on colorectal cancer patient prognosis and survival.	epidermal growth factor receptor (egfr) is overexpressed in many types of cancers, especially colorectal cancer (crc), and seems to reflect more aggressive histological and clinical behaviors. the aim of this study was to evaluate egfr immunohistochemical reactivity in crc biopsies, and to analyze its relationship with various histological and clinical characteristics and survival.	2005	15598946
esophagectomy: is it necessary after chemoradiotherapy for a locally advanced t4 esophageal cancer? prospective nonrandomized trial comparing chemoradiotherapy with surgery versus without surgery.	the need for surgery after chemoradiotherapy for a t4n0-1m0 squamous cell carcinoma in the thoracic esophagus was evaluated. a series of 53 patients were enrolled in this prospective nonrandomized trial from among 124 patients with an esophageal cancer assessed as t4 in kurume university hospital from 1994 to 2002. after the first chemoradiotherapy cycle, which consisted of radiotherapy in a total dosage of 36 gy and chemotherapy using cisplatin (cddp) and 5-fluorouracil (5fu), the patients each ...	2005	15599735
endocrine and metabolic changes in anguilla anguilla l. following exposure to beta-naphthoflavone--a microsomal enzyme inducer.	anguilla anguilla l. were exposed during 24 and 48 h to 2.7 mum beta-naphthoflavone (bnf), a known microsomal enzyme inducer. the bnf effects on thyroid-stimulating hormone (tsh), free triiodothyronine (t3), free thyroxine (t4) and cortisol plasma levels were investigated. alterations on plasma glucose and lactate levels were also measured as an indication of energy-mobilizing hormones alterations. bnf showed to be able to decrease significantly a. anguilla plasma t4 levels, whereas tsh, t3 and ...	2005	15607783
a pilot study of high-dose intraarterial cisplatin chemotherapy with concomitant accelerated radiotherapy for patients with previously untreated t4 and selected patients with t3n0-n3m0 squamous cell carcinoma of the upper aerodigestive tract.	this phase i clinical trial was developed to assess the feasibility of combining high-dose intraarterial cisplatin chemotherapy with concurrent, concomitant boost accelerated radiation therapy for patients with previously untreated t4 and select patients with t3n0-n3m0 squamous cell carcinoma of the upper aerodigestive tract.	2005	15597408
current tnm classification of renal cell carcinoma evaluated: revising stage t3a.	: recent studies of rare cases of pt3a renal cell carcinoma extending directly into the adrenal gland showed worse survival than in other pt3a cases and recategorization as stage pt4 was suggested. we assessed the prognostic validity of a stage pt3a diagnosis based on perirenal fat infiltration.	2005	15592020
women with low libido: correlation of decreased androgen levels with female sexual function index.	the aim of the present study was to investigate a possible correlation between decreased androgen levels and female sexual function index (fsfi) in women with low libido and compare these findings with normal age-matched subjects. in total, 20 premenopausal women with low libido (mean age 36.7; range 24-51 y) and 20 postmenopausal women with low libido (mean age 54; 45-70 y), and 20 premenopausal healthy women (mean age 32.2; range 21-51 y) and 20 postmenopausal healthy women (mean age 53.5; ran ...	2005	15592425
pcbs and ddt in the serum of juvenile california sea lions: associations with vitamins a and e and thyroid hormones.	top-trophic predators like california sea lions bioaccumulate high levels of persistent fat-soluble pollutants that may provoke physiological impairments such as endocrine or vitamins a and e disruption. we measured circulating levels of polychlorinated biphenyls (pcbs) and dichlorodiphenyltrichloroethane (ddt) in 12 healthy juvenile california sea lions captured on año nuevo island, california, in 2002. we investigated the relationship between the contamination by pcbs and ddt and the circulati ...	2005	15589659
antiandrogenic effects in short-term in vivo studies of the fungicide fenarimol.	the fungicide fenarimol has estrogenic and antiandrogenic activity and inhibits aromatase activity in vitro. we tested, whether fenarimol had antiandrogenic effects in vivo. in a hershberger assay, fenarimol given orally to castrated testosterone-treated male rats caused markedly reduced weights of ventral prostate, seminal vesicles, musc. levator ani/bulbocavernosus, and bulbourethral glands. qualitatively similar, but weaker, effects were also evident in intact fenarimol-exposed young adult ma ...	2005	15590119
serum thymidine kinase 1 is a prognostic and monitoring factor in patients with non-small cell lung cancer.	thymidine kinase 1 (tk1) is a pyrimidine metabolic pathway enzyme involved in salvage dna synthesis and thus, a cell cycle-dependent marker. we have developed anti-tk1 monoclonal/polyclonal abs raised against a 15-amino acid synthetic peptide (kpgeavaarklfapq) corresponding to part of the c-terminus of human tk1. these abs are useful for both serological and immunohistochemical detection of tk1 in a number of cancer diseases. in this study we examined tk1 concentration in serum (stk1) in 250 pre ...	2005	15583816
comparison of plain and hyperbaric solutions of ropivacaine for spinal anaesthesia.	preliminary work has shown that ropivacaine provides spinal anaesthesia of shorter duration than bupivacaine, and may be of particular use in the day-case setting. however, there are few data comparing the actions of plain and hyperbaric solutions of this drug.	2005	15516352
patient-rated emotional and physical functioning among hematologic cancer patients during hospitalization for stem-cell transplantation.	in this 3-year prospective inpatient study, 220 patients received stem-cell transplantation (sct) for hematologic cancer at a single institution. the objective of the study is to provide data on patient-rated emotional (depression and anxiety) and physical (overall physical status, energy level, and systemic symptomatology) functioning during hospitalization for sct and to compare whether these differ between autologous and allogeneic sct. patients were assessed at hospital admission (t1), day o ...	2005	15580279
postoperative radiotherapy for primary mucosal melanoma of the head and neck.	primary head and neck mucosal melanoma (hnmm) has a poor prognosis with a low local control rate and frequent distant metastases. the objective of the current study was to determine the impact of postoperative radiotherapy on local control and survival.	2005	15578718
the n terminus of the head protein of t4 bacteriophage directs proteins to the groel chaperonin.	the head protein of t4 bacteriophage requires the groel chaperonin for its insertion into a growing t4 head. hundreds of thousands of copies of this protein must pass through the chaperonin in a limited time later in infection, indicating that the protein must use groel very efficiently and may contain sequences that bind tightly to groel. we show that green fluorescent protein (gfp) fused to the n terminus of the head protein can fold at temperatures higher than those at which the gfp protein c ...	2005	15571729
the intra-push velocity profile of the over-ground racing wheelchair sprint start.	the aim of this study was to analyse the first six pushes of a sprint start in over-ground racing wheelchair propulsion. one international male wheelchair athlete (age=28 years; body mass=60.6 kg; racing classification=t4) performed maximal over-ground sprint trials, over approximately 10 m, in his own racing wheelchair fitted with a velocometer. each trial was filmed at 200 hz using a "pan and tilt" system. eight trials were manually digitised at 100 hz. raw co-ordinate data were smoothed and d ...	2005	15519335
role of the constitutive androstane receptor in xenobiotic-induced thyroid hormone metabolism.	the induction of hepatic drug metabolizing enzymes alters not only the metabolism of the xenobiotic substances that induce them but also the metabolism of various endogenous hormones. the xenobiotic receptor constitutive androstane receptor (car) (nr1i3) mediates the well-studied induction of cyp2b genes and other drug-metabolizing enzymes by phenobarbital (pb), an antiepileptic drug that has been shown to alter thyroid hormone (th) levels. here we show that car is required for pb-mediated disru ...	2005	15564320
p-site pairing subtleties revealed by the effects of different trnas on programmed translational bypassing where anticodon re-pairing to mrna is separated from dissociation.	programmed ribosomal bypassing occurs in decoding phage t4 gene 60 mrna. half the ribosomes bypass a 50 nucleotide gap between codons 46 and 47. peptidyl-trna dissociates from the "take-off" gga, codon 46, and re-pairs to mrna at a matched gga "landing site" codon directly 5' of codon 47 where translation resumes. the system described here allows the contribution of peptidyl-trna re-pairing to be measured independently of dissociation. the matched gga codons have been replaced by 62 other matche ...	2005	15567409
binding of histone h1 to dna is described by an allosteric model.	equilibrium binding data were analyzed to characterize the interaction of the linker histone h1 degrees with unmodified t4 phage dna. data were cast into the scatchard-type plot described by mcghee and von hippel and fit to their eponymous model for nonspecific binding of ligand to dna. the data were not fit by the simple mcghee-von hippel model, nor fit satisfactorily by the inclusion of a cooperativity parameter. instead, the interaction appeared to be well described by crothers' allosteric mo ...	2005	15558656
daily variations in type ii iodothyronine deiodinase activity in the rat brain as controlled by the biological clock.	type ii deiodinase (d2) plays a key role in regulating thyroid hormone-dependent processes in, among others, the central nervous system (cns) by accelerating the intracellular conversion of t4 into active t3. just like the well-known daily rhythm of the hormones of the hypothalamo-pituitary-thyroid axis, d2 activity also appears to show daily variations. however, the mechanisms involved in generating these daily variations, especially in the cns, are not known. therefore, we decided to investiga ...	2005	15550511
definitive radiotherapy with interstitial implant boost for squamous cell carcinoma of the tongue base.	the purpose of this study was to examine the long-term outcome of a cohort of patients with unresected base of tongue carcinoma who received interstitial brachytherapy after comprehensive external beam radiation therapy.	2005	15726587
survival outcome of patients with nasopharyngeal carcinoma with first local failure: a study by the hong kong nasopharyngeal carcinoma study group.	the purpose of this article is to report the overall survival (os) outcome of patients with nasopharyngeal carcinoma (npc) with local failure who received salvage treatment and to identify prognostic factors for os.	2005	15726589
generation of long tracts of disease-associated dna repeats.	the generation of long uninterrupted dna repeats is important for the study of repeat instability associated with several human genetic diseases, including myotonic dystrophy type 1. however, obtaining defined lengths of long repeats in vitro has been problematic. strand slippage and/or dna secondary structure formation may prevent efficient ligation. for example, a purified (ctg)140.(cag)140 repeat fragment containing 4-bp agca/tgct overhanging ends ligated poorly using t4 or escherichia coli d ...	2005	15727131
improvement of chronic idiopathic urticaria with l-thyroxine: a new tsh role in immune response?	the association between chronic idiopathic urticaria (ciu) and autoimmune thyroiditis (at) is known, as well as major prevalence of antithyroid antibodies in the allergical subjects and other autoimmune diseases. we have evaluated the effects of l-thyroxine on clinical symptoms of ciu in at patients suggesting the hypothesis of a new thyroid-stimulating hormone (tsh) role in immune system.	2005	15727581
sexual dimorphism of thyroid function in newborns with congenital hypothyroidism.	several characteristics of congenital hypothyroidism (ch) from thyroid dysgenesis (ectopy and athyreosis) are sexually dimorphic: girls are more often affected, and boys are twice more likely than girls to have absent knee epiphysis at diagnosis, an indicator of severity of ch. whether the biochemical severity of ch is sexually dimorphic is unknown. we therefore reviewed the charts of all newborns referred to our clinic from 1990 to 2004 because of a tsh greater than 15 mu/liter on newborn scree ...	2005	15728201
assembly of the bacteriophage t4 primosome: single-molecule and ensemble studies.	within replisomes for dna replication, the primosome is responsible for unwinding double-stranded dna and synthesizing rna primers. assembly of the bacteriophage t4 primosome on individual molecules of ssdna or forked dna (fdna) has been studied by using fret microscopy. on either dna substrate, an ordered process of assembly begins with tight 1:1 binding of ssdna-binding protein (gp32) and helicase-loading protein (gp59) to the dna. magnesium adenosine 5'-o-(3-thiotriphosphate) (mgatpgammas) me ...	2005	15728347
thyroxine-binding antibodies inhibit t cell recognition of a pathogenic thyroglobulin epitope.	thyroid hormone-binding (thb) abs are frequently detected in autoimmune thyroid disorders but it is unknown whether they can exert immunoregulatory effects. we report that a thb mab recognizing the 5' iodine atom of the outer phenolic ring of thyroxine (t4) can block t cell recognition of the pathogenic thyroglobulin (tg) peptide (2549-2560) that contains t4 at aa position 2553 (t4(2553)). following peptide binding to the mhc groove, the thb mab inhibited activation of the a(k)-restricted, t4(25 ...	2005	15728526
gender differences in growth hormone response to exercise before and after rhgh administration and the effect of rhgh on the hormone profile of fit normal adults.	exercise is a potent physiological stimulus of gh secretion. we hypothesized that exogenous recombinant human growth hormone (rhgh) administration through an increase in gh and igf-i levels would blunt the gh response to exercise. the aim of the study was to examine and compare the impact of rhgh on the exercise-induced gh response in healthy normal men and women.	2005	15730413
sentinel lymph node biopsy in the management of cutaneous head and neck melanoma.	sentinel lymph node biopsy has revolutionized the surgical management of primary malignant melanoma. most series on sentinel lymph node mapping have concentrated on extremity and truncal melanomas. the head and neck region has a rich and unpredictable lymphatic system. the use of sentinel lymph node mapping in the management of head and neck melanoma is evaluated. the authors conducted a retrospective review of patients treated for clinical stage i and stage ii malignant melanoma of the head and ...	2005	15731669
impact of iodine supplementation of dairy cows on milk production and iodine concentrations in milk.	to determine the effect of iodine supplementation on milk production and iodine concentrations in milk for pasture-fed, seasonally calving dairy cows.	2005	15731828
effect of grazing pasture with a low selenium content on the concentrations of triiodothyronine and thyroxine in serum, and gsh-px activity in erythrocytes in cows in chile.	to determine the effect of grazing pasture that had a low selenium (se) concentration on serum concentrations of triiodothyronine (t3) and thyroxine (t4), and erythrocyte glutathione peroxidase (gsh-px) activity in dairy cows.	2005	15731838
expression and localization of caveolins during postnatal development in rat heart: implication of thyroid hormone.	caveolins modulate signaling pathways involved in cardiac development. caveolin-1 exists in two isoforms: the beta-isoform derivates from an alternative translational start site that creates a protein truncated by 31 amino acids, mainly expressed in endothelial cells, whereas caveolin-3 is present in muscle cells. our aim was to define caveolin distribution and expression during cardiac postnatal development using immunofluorescence and western blotting. caveolin-3 sarcolemmal labeling appeared ...	2005	15718400
2004 asm conference on the new phage biology: the 'phage summit'.	in august, more than 350 conferees from 24 countries attended the asm conference on the new phage biology, in key biscayne, florida. this meeting, also called the phage summit, was the first major international gathering in decades devoted exclusively to phage biology. what emerged from the 5 days of the summit was a clear perspective on the explosive resurgence of interest in all aspects of bacteriophage biology. the classic phage systems like lambda and t4, reinvigorated by structural biology, ...	2005	15720541
protein-dna interactions in the t4 dntp synthetase complex dependent on gene 32 single-stranded dna-binding protein.	our laboratory has reported data suggesting a role for t4 phage gene 32 single-stranded dna-binding protein in organizing a complex of deoxyribonucleotide-synthesizing enzymes at the replication fork. in this article we examined the effects of gene 32 ablation on the association of these enzymes with dna-protein complexes. these experiments showed several deoxyribonucleotide-synthesizing enzymes to be present in dna-protein complexes, with some of these associations being dependent on gene 32 pr ...	2005	15720556
initiation of repair of dna-polypeptide cross-links by the uvrabc nuclease.	although the biochemical pathways that repair dna-protein cross-links have not been clearly elucidated, it has been proposed that the partial proteolysis of cross-linked proteins into smaller oligopeptides constitutes an initial step in removal of these lesions by nucleotide excision repair (ner). to test the validity of this repair model, several site-specific dna-peptide and dna-protein cross-links were engineered via linkage at (1) an acrolein-derived gamma-hydroxypropanodeoxyguanosine adduct ...	2005	15723543
age-related differences of ascaridia galli egg output and worm burden in chickens following a single dose infection.	ninety white chickens (lohmann lsl) were reared under helminth-free conditions and divided into five groups. four groups were artificially infected with 250 embryonated ascaridia galli eggs at the age of 6, 12, 18 or 24 weeks. ten birds were kept as uninfected controls. six and 10 weeks after infection (p.i.), individual faecal egg counts (fec) were performed. the birds were slaughtered after the second sampling and their gastrointestinal tracts were examined for the presence of adult a. galli. ...	2005	15725544
ziprasidone-induced galactorrhea: a case report.	second-generation antipsychotics presumably lack the typical side effects of conventional antipsychotics.	2005	15726024
control of bacteriophage t4 tail lysozyme activity during the infection process.	bacteriophage t4 has an efficient mechanism for injecting the host escherichiacoli cell with genomic dna. its gene product 5 (gp5) has a needle-like structure attached to the end of a tube through which the dna passes on its way out of the head and into the host. the gp5 needle punctures the outer cell membrane and then digests the peptidoglycan cell wall in the periplasmic space. gp5 is normally post-translationally cleaved between residues 351 and 352. the function of this process in controlli ...	2005	15701513
down-regulation of s100c is associated with bladder cancer progression and poor survival.	the goal of this study was to identify proteins down-regulated during bladder cancer progression. experimental design: by using comparative proteome analysis and measurement of mrna, we found a significant down-regulation of s100c, a member of the s100 family of proteins, in t24 (grade 3) as compared with rt4 (grade 1) bladder cancer cell lines. moreover, quantification of the mrna level revealed that decreased expression of the protein reflects a low level of transcription of the s100c gene. ba ...	2005	15701847
antenatal glucocorticoids increase early total thyroxine levels in premature infants.	hypothyroxinemia is associated with adverse neonatal outcomes including white matter damage, cerebral palsy, poor neurodevelopment and death. it has become increasingly important to understand the natural history and modifiers of thyroid function in the premature infant. it is standard obstetrical practice to offer antenatal glucocorticoids to pregnant women with threatened preterm delivery. few studies have investigated the effect of antenatal glucocorticoids on neonatal thyroid function.	2005	15703468
effects of novel females and stage of the estrous cycle on sexual behavior in mature beef bulls.	the objective of this experiment was to determine the effects of unrestrained females on sexual behavior of bulls. twelve angus bulls were used in three latin square replicates where sexual interactions between one bull and one female were quantified for each of four 60-min tests (t1, t2, t3, and t4, respectively). all bulls received the following treatments: 1) exposure to four estrual females in sequence (a-b-c-d); 2) exposure to two estrual females in alternating sequence (e-f-e-f); 3) exposu ...	2005	15705758
effects of circuit training on body composition and peak cardiorespiratory responses in patients with moderate to severe traumatic brain injury.	to examine the time course of the changes in body composition and peak cardiorespiratory fitness resulting from routine brain injury rehabilitation program (birp) activities and circuit training in patients with moderate to severe traumatic brain injury (tbi).	2005	15706553
characterization of a wide range base-damage-endonuclease activity of mammalian rps3.	mammalian rps3, a ribosomal protein s3 with a dna repair endonuclease activity, nicks heavily uv-irradiated dna and dna containing ap sites. rps3 calls for a novel endonucleolytic activity on ap sites generated from pyrimidine dimers by t4 pyrimidine dimer glycosylase activity. this study revealed that rps3 cleaves the lesions including ap sites, thymine glycols, and other uv damaged lesions such as pyrimidine dimers. this enzyme does not have a glycosylase activity as predicted from its amino a ...	2005	15707971
posttreatment tnm staging is a prognostic indicator of survival and recurrence in tethered or fixed rectal carcinoma after preoperative chemotherapy and radiotherapy.	to evaluate the prognostic value of the posttreatment tnm stage as a predictor of outcome in locally advanced rectal cancers treated with preoperative chemotherapy and radiotherapy.	2005	15708244
biological activities of conjugated fatty acids: conjugated eicosadienoic (conj. 20:2delta(c11,t13/t12,c14)), eicosatrienoic (conj. 20:3delta(c8,t12,c14)), and heneicosadienoic (conj. 21:2delta(c12,t14/c13,t15)) acids and other metabolites of conjugated linoleic acid.	the elongated form of conjugated linoleic acid (cla), conjugated eicosadienoic acid (cea, conj. 20:2delta(c11,t13/t12,c14)), was generated from cla by liver microsomal fractions. subsequent testing showed that dietary cea significantly reduced body fat, and increased lean mass similar to cla when compared to controls. cea also decreased lipoprotein lipase activity and triacylglyceride, and increased glycerol release in 3t3-l1 adipocytes, correlated with the trans-12,cis-14 isomer, but cea requir ...	2005	15708360
interaction between the t4 helicase-loading protein (gp59) and the dna polymerase (gp43): a locking mechanism to delay replication during replisome assembly.	the t4 helicase-loading protein (gp59) has been proposed to coordinate leading- and lagging-strand dna synthesis by blocking leading-strand synthesis during the primosome assembly. in this work, we unambiguously demonstrate through a series of biochemical and biophysical experiments, including single-molecule fluorescence microscopy, that the inhibition of leading-strand holoenzyme progression by gp59 is the result of a complex formed between gp59 and leading-strand polymerase (gp43) on dna that ...	2005	15709743
evaluation of different culture systems on the in vitro production of bovine embryos.	the objective of this study was to determine the development potential and quality of in vitro produced bovine embryos cultured individually or in groups. after ivm and ivf, presumptive zygotes were cultured in groups or individually, either in drops or in the modified "well of the well" (mwow) system. in experiment 1, four culture systems were utilized: t1: drop in group (control); t2: mwow in groups; t3: mwow individually; and t4: drop individually. cleavage and blastocyst rates at days 6, 7 a ...	2005	15710198
acute licl-treatment affects the cytoplasmic t4 availability and the expression pattern of thyroid hormone receptors in adult rat cerebral hemispheres.	we have previously reported that short-term licl-treatment affects the kinetic characteristics of thyroid hormone binding in adult rat brain (bolaris, s., margarity, m., valcana, t., 1995. effects of licl on triiodothyronine (t3) binding to nuclei from rat cerebral hemispheres. biol. psychiatry 37, 106-111); however, the mechanism underlying the above effects of licl administration is yet to be determined. in this study, the effects of lithium within one day after its administration (5 mmol/kg b ...	2005	15710487
preoperative radiochemotherapy and radical resection for stages ii-iv oral and oropharyngeal cancer: outcome of 222 patients.	to analyse survival and locoregional control in patients with advanced oral and oropharyngeal squamous cell carcinoma (scc) after multimodal therapy with preoperative radiochemotherapy (rct) and radical surgery. we included in this analysis 222 patients who underwent multimodal therapy between 1990 and 2000. eligible were patients with uicc disease stages ii-iv (t2: 33.3%; t3: 12.6%; t4: 54.1%; n0: 45.9%; n1: 17.6%; n2: 33.3%; n3: 3.2%; stage ii: 21.1%; stage iii: 14.9%; stage iv: 64%). patients ...	2005	15695042
prognostic relevance of cd105+ microvessel density in hnscc patient outcome.	angiogenesis is essential for the development and progression of malignant tumours, and there is increasing evidence that microvessel density (mvd) can be considered an indirect marker of neo-angiogenesis. however, there is still disagreement concerning the clinical relevance and prognostic significance of mvd in head and neck squamous cell carcinomas (hnsccs). mvd was evaluated in 127 hnscc patients by means of immunohistochemistry using monoclonal antibodies (mabs) against cd34 and cd105 (endo ...	2005	15695116
[a family with familial dysalbuminaemic hyperthyroxinaemia].	to report a family of familial dysalbuminaemic hyperthyroxinaemia(fdh).	2005	15696477