Publications

pseudoaneurysm of the thoracic radiculomedullary artery with subarachnoid hemorrhage. case report.	the authors report a rare case of a patient with a left-sided t-5 radiculomedullary artery pseudoaneurysm who presented with spinal subarachnoid hemorrhage (sah). the patient, a 71-year-old woman, was hospitalized for progressive paraplegia and sensory loss with bladder and rectal dysfunction. computerized tomography scanning revealed an sah at the posterior fossa. spinal t2-weighted magnetic resonance imaging demonstrated sah and an inhomogeneous and slightly low signal intensity mass at t4-5. ...	2004	15029924
[intraarterial chemotherapy with bladder preservation in patients with invasive bladder carcinoma].	intraarterial chemotherapy (iac) was carried out on patients with invasive bladder carcinoma to treat the bladder carcinoma while preserving the bladder. fifteen patients with bladder carcinoma at stage t2-t4 were treated with intraarterial cisplatin (cddp: 70 mg/m2) and adriamycin (adm: 30 mg/m2) every 3 to 4 weeks. the response was observed in all 15 patients. ten (66.7%) achieved a complete response (cr), and 3 (20.0%) obtained a partial response (pr). with a mean follow-up of 22.6 months, th ...	2004	15032009
very fast folding and association of a trimerization domain from bacteriophage t4 fibritin.	the foldon domain constitutes the c-terminal 30 amino acid residues of the trimeric protein fibritin from bacteriophage t4. its function is to promote folding and trimerization of fibritin. we investigated structure, stability and folding mechanism of the isolated foldon domain. the domain folds into the same trimeric beta-propeller structure as in fibritin and undergoes a two-state unfolding transition from folded trimer to unfolded monomers. the folding kinetics involve several consecutive rea ...	2004	15033360
inhibition of the activity of the native gamma-aminobutyric acid a receptor by metabolites of thyroid hormones: correlations with molecular modeling studies.	to characterize the direct effects of thyroid hormones on native gamma-aminobutyric acid(a) (gaba(a)) receptors, rapid (5 s) actions of a series of iodothyronines on muscimol-stimulated uptake of (36)cl(-) were investigated in synaptoneurosomes prepared from rat brain. the results were correlated with molecular modeling of the active compounds. dose-response curves for muscimol in the presence of 3,3', 5-l-triiodothyronine (l-t3) indicated a noncompetitive inhibition of muscimol-stimulated (36)c ...	2004	15033424
increased aberrance of cytokine expression in plasma of patients with more advanced squamous cell carcinoma of the head and neck.	patients with head and neck squamous cell carcinoma (hnscc) are biased toward the th2 phenotype as they have increased levels of the th2 cytokines il-4, il-6 and il-10 and diminished levels of the th1 cytokine ifn-gamma. however, this bias is incomplete since levels of the th1 cytokines il-2 and gm-csf are increased. this study examined the interrelationship among the plasma levels of th1 and th2 cytokines in 101 hnscc patients and 40 age-matched controls without a known malignancy. control subj ...	2004	15036248
induction of a new follicular wave in holstein heifers synchronized with norgestomet.	treatments with progestin to synchronize the bovine estrous cycle in the absence of the corpus luteum, induces persistence of a dominant follicle and a reduction of fertility at doses commonly utilized. the objective of the present research was to induce a new wave of ovarian follicular development in heifers in which stage of the estrous cycle was synchronized with norgestomet. holstein heifers (n=30) were used, in which estrus was synchronized using two doses of pgf2alpha i.m. (25 mg each) 11 ...	2004	15036514
prognosis of muscle-invasive bladder cancer: difference between primary and progressive tumours and implications for therapy.	to evaluate the difference in prognosis between progressive and primary muscle-invasive bladder cancer.	2004	15036673
in vitro development of preimplantation porcine nuclear transfer embryos cultured in different media and gas atmospheres.	this study investigated the effect of culture media and gas atmospheres on the development of porcine nuclear transfer embryos. oocytes derived from a local abattoir were matured for 42-44 h and enucleated. fetal fibroblasts were prepared from a day 35 porcine fetus. confluent stage fetal fibroblasts were introduced into the perivitelline space of enucleated oocytes. fusion and activation were induced simultaneously with two direct current (1.2 kv/cm for 30 micros) in 0.3 m mannitol medium. for ...	2004	15037000
inactivation of the 3'-5' exonuclease of the replicative t4 dna polymerase allows translesion dna synthesis at an abasic site.	here, we have investigated the consequences of the loss of proof-reading exonuclease function on the ability of the replicative t4 dna polymerase (gp43) to elongate past a single abasic site located on model dna substrates. our results show that wild-type t4 dna polymerase stopped at the base preceding the lesion on two linear substrates having different sequences, whereas the gp43 d219a exonuclease-deficient mutant was capable of efficient bypass when replicating the same substrates. the struct ...	2004	15037066
surgery for t4 non-small cell lung cancer.		2004	15037295
the neurospora crassa cyt-18 protein c-terminal rna-binding domain helps stabilize interdomain tertiary interactions in group i introns.	the neurospora crassa mitochondrial tyrosyl-trna synthetase (cyt-18 protein) promotes the splicing of group i introns by stabilizing the catalytically active rna structure. to accomplish this, cyt-18 recognizes conserved structural features of group i intron rnas using regions of the n-terminal nucleotide-binding fold, intermediate alpha-helical, and c-terminal rna-binding domains that also function in binding trna(tyr). curiously, whereas the splicing of the n. crassa mitochondrial large subuni ...	2004	15037773
practical and general synthesis of 5'-adenylated rna (5'-apprna).	a simple strategy is reported for 5'-adenylation of nearly any rna sequence of indefinite length. the 5'-adenylated product (5'-apprna) is an activated rna that is structurally similar to 5'-triphosphorylated rna, which is usually prepared by in vitro transcription using t7 rna polymerase. in the new 5'-adenylation strategy, the rna substrate is first 5'-monophosphorylated either by t4 polynucleotide kinase, by in vitro transcription in the presence of excess gmp, or by appropriate derivatizatio ...	2004	15037782
generation of optimized yellow and red fluorescent proteins with distinct subcellular localization.	fluorescent proteins (fps) have revolutionized many aspects of cell biology and have become indispensable research tools. today's increasingly complex experiments aiming to understand biological systems strongly depend on the availability of combinations of multiple fps, which allow their distinguishable simultaneous detection in the same cell or tissue. recently, the venus and dsred. t4 fps were described as the latest generation of yellow and red fps. to increase the combinatorial possibilitie ...	2004	15038157
new drosophila transgenic reporters: insulated p-element vectors expressing fast-maturing rfp.	in vivo green fluorescent protein (gfp)/red fluorescent protein (rfp) double-labeling studies have been hampered by several inconvenient properties of dsred, the first described rfp. these disadvantages include a very slow (> 24 h) maturation time, emission of contaminating green light, and low solubility. a recently developed variant of dsred, called dsred.t4, has a much shorter maturation time, no significant green emission, and improved solubility. we have constructed drosophila p-element tra ...	2004	15038159
administration of a single dose of recombinant human thyrotrophin enhances the efficacy of radioiodine treatment of large compressive multinodular goitres.	patients with very large multinodular goitres, frequently found among elderly people, often suffering from cardiovascular or other disabling disorders, may be considered as unsuitable for surgery. we have evaluated the feasibility of relatively high-dose 131i therapy in such patients. as subclinical or clinical hyperthyroidism is commonly found in these patients, associated with a low radioiodine (rai) uptake at 24 h, we pretreated a group of patients with a single intramuscular injection of rec ...	2004	15008994
thyroid function in girls with epilepsy with carbamazepine, oxcarbazepine, or valproate monotherapy and after withdrawal of medication.	antiepileptic drugs may affect the serum thyroid hormone concentrations. the aim of this study was to evaluate thyroid function in 78 girls taking carbamazepine (cbz), oxcarbazepine (oxc), or valproate (vpa) monotherapy for epilepsy and after withdrawal of the treatment.	2004	15009219
differentiated thyroid carcinoma: the new uicc 6th edition tnm classification system in a retrospective analysis of 169 patients.	to compare the new, 6th edition, uicc tnm staging system with the former edition, we updated tnm staging in patients with differentiated thyroid carcinoma.	2004	15009916
expression of smac/diablo is a novel prognostic marker in lung cancer.	recently smac/diablo (second mitochondria-derived activator of caspase) has been identified as a proapoptotic protein that inhibits iaps (inhibitors of apoptosis proteins). smac is expressed ubiquitously in many organs, including the lung. here we evaluated the expression of smac mrna with real-time reverse-transcription pcr in 88 primary lung cancers and matched normal tissues. smac mrna expression in tumor tissues was significantly lower than that in normal tissues (p<0.0001). in squamous cell ...	2004	15010875
k-ras and p16(ink4a)alterations in sputum of nsclc patients and in heavy asymptomatic chronic smokers.	nsclc rates among the most frequent and lethal neoplasm world-wide and a significant decrease in morbidity and mortality relies only upon effective early diagnostic strategies. we investigated k-ras mutations and p16(ink4a) hypermethylation in tumor tissue and sputum of 50 patients with nsclc and correlated them with sputum cytology and with tumor staging, grading and location, to ascertain, in sputum, their potential diagnostic impact. the same genetic/epigenetic abnormalities and cytological f ...	2004	15013580
serum levels of interleukin-18 and nitrite+nitrate in renal cell carcinoma patients with different tumor stage and grade.	interleukin (il-18) and nitric oxide (no) are immunoregulatory cytokines that have been suggested to participitate in the multistep process of carcinogenesis. in this study, serum il-18 and nitrite+nitrate levels (as an index of no generation) were measured in 26 patients with histologically confirmed renal cell carcinoma (rcc) before surgical procedure and in 8 healthy controls. rcc patients showed significantly higher serum il-18 levels when compared to the control subjects (p<0.001). serum il ...	2004	15004655
diffusion constant in gel electrophoresis at high fields.	we present new measurements of the diffusion constant d in standard (slab-gel) electrophoresis of dna at fields up to 10 v/cm. molecules investigated are bacteriophages: t4 of length 173 kbp and lambda of length 48.5 kbp cut by restriction enzyme hindiii. we show, that d increases with the molecule length for electric field e above 5 v/cm. the results are interpreted within the geometration model.	2004	15004836
diagnostic effectiveness of simultaneous thyroxine and thyroid-stimulating hormone screening measurements. thirteen years' experience in the northeast italian screening programme.	to evaluate the effectiveness of thyroid-stimulating hormone (tsh) and thyroxine (t4) measurements at neonatal screening for congenital hypothyroidism, we compared our false-negative results with those we would have obtained if we had used tsh screening alone.	2004	15006107
[two younger male patients with rapidly progressing pulmonary pleomorphic carcinoma].	we report 2 cases of pulmonary pleomorphic carcinoma. the patient in case 1 was a 44-year-old man who was admitted to our hospital complaining of dry cough and dyspnea on exertion. chest radiography and ct showed a huge tumor with left pleural effusion. percutaneous biopsy suggested malignancy. as distant metastasis was not found and as cytological examination of pleural effusion gave a result of class ii, we attempted to perform left pneumonectomy. however, we performed partial resection of the ...	2004	15007917
competitive adsorption of bacteriophage t4 lysozyme stability variants at hydrophilic glass surfaces.	the competitive adsorption behavior exhibited by the wild-type t4 lysozyme and two of its structural stability variants was studied by 125i radioisotope labeling. the mutant lysozymes were produced by substitution of the isoleucine residue at position 3 in the wild type with a tryptophan residue, resulting in a protein with lower structural stability, or with a cysteine residue, resulting in a protein with higher structural stability. adsorption kinetics were recorded for binary protein mixtures ...	2004	14651918
graves' hyperthyroidism and thyroiditis in hla-drb1*0301 (dr3) transgenic mice after immunization with thyrotropin receptor dna.	familial and twin studies in caucasians have established that the mhc class ii allele hla-drb1*0301 (dr3) is a strong susceptibility gene in graves' hyperthyroid disease (gd). to determine if a dr3 transgene could help establish an animal model for gd, we expressed dr3 molecules in class ii-knockout nod mice (h2ag7-). dr3+g7- mice were given cardiotoxin prior to immunization on weeks 0, 3 and 6 with plasmid dna encoding human thyrotropin receptor (tshr). two groups of mice were also coimmunized ...	2004	14678262
n-terminal-pro-b-type natriuretic peptide (nt-pro-bnp) in different thyroid function states.	n-terminal b-type natriuretic peptide (nt-pro-bnp) is secreted from the cardiac ventricles in response to volume expansion and pressure overload, and serum levels are elevated in systolic heart failure. the aim of this study was to evaluate the influence of thyroid function on nt-pro-bnp.	2004	14678288
radiotherapy and hyperthermia in the treatment of patients with locally advanced prostate cancer: preliminary results.	to report an interim clinical evaluation of combined external beam irradiation (ebrt) and interstitial or regional hyperthermia in the treatment of locally advanced prostate cancer.	2004	14678364
association between eeg alpha power and visuospatial function in obsessive-compulsive disorder.	the purpose of the present paper was to determine if frontal activity, measured as electroencephalogram alpha power, correlates with visuospatial functions in obsessive-compulsive disorder (ocd). electroencephalography and the rey-osterrieth complex figure test (rcft) were performed on 23 patients meeting the diagnostic and statistical manual of mental disorders (4th edn; dsm-iv) ocd criteria. after quantitatively analyzing eeg recordings taken over the frontal, temporal, parietal and occipital ...	2004	14678451
long-term validity of biological markers of psychopathy and criminal recidivism: follow-up 6-8 years after forensic psychiatric investigation.	this study is a follow-up investigation of a forensic psychiatric sub-population 6-8 years after forensic psychiatric evaluation. the aim was to examine the long-term validity of biological markers of psychopathy and antisocial behavior over time. data on criminal records were obtained at follow-up from the national council for crime prevention. basic data included findings of psychiatric and psychological assessments, as well as values for serum triiodothyronine (t3) and free thyroxin (ft4), an ...	2004	14675747
development of a terrestrial vertebrate model for assessing bioavailability of cadmium in the fence lizard (sceloporus undulatus) and in ovo effects on hatchling size and thyroid function.	in the terrestrial environment, standardized protocols are available for measuring the exposure and effects of contaminants to invertebrates, but none currently exist for vertebrates. in an effort to address this, we proposed that developing lizard embryos may be used as a terrestrial vertebrate model. lizard eggs may be particularly susceptible to soil contamination and in ovo exposure may affect hatchling size, mortality, as well as thyroid function. toxicant-induced perturbations of thyroid f ...	2004	14675843
mutations in a conserved motif inhibit single-stranded dna binding and recombination mediator activities of bacteriophage t4 uvsy protein.	the uvsy recombination mediator protein is critical for homologous recombination in bacteriophage t4. uvsy uses both protein-protein and protein-dna interactions to mediate the assembly of the t4 uvsx recombinase onto single-stranded (ss) dna, forming presynaptic filaments that initiate dna strand exchange. uvsy helps uvsx compete with gp32, the t4 ssdna-binding protein, for binding sites on ssdna, in part by destabilizing gp32-ssdna interactions, and in part by stabilizing uvsx-ssdna interactio ...	2004	14634008
perinatal dioxin exposure and later effects--a review.	negative effects of perinatal exposure to background levels of dioxins and pcbs in europe and the usa have been documented. four facets of development are reviewed in this paper: 1. brain development and thyroid hormone metabolism. 2. hepatic effects. 3. hematopoietic system effects. 4. lung function. effects on iq and behaviour have been documented in children on both sides of the atlantic ocean. non-dioxin-like pcbs, measured in maternal and cord blood and current plasma samples have been impl ...	2004	14659426
thyroid hormone resistance and enlargement of the sella turcica during pregnancy.	case report: a 26-year-old pregnant woman was admitted to our institution running her 30 weeks of gestation. the patient had a past history of total thyroidectomy cause of a thyroid papillary carcinoma and presented with increased supraphysiological tsh levels under 250 microg t4, while slightly hyperthyroid, from the clinical point of view. partial resistance to thyroid replacement therapy or tsh-secreting tumour was evoked. pituitary mri revealed a pituitary enlargement without excluding a pit ...	2004	14648184
analysis of the lambdoid prophage element e14 in the e. coli k-12 genome.	many sequenced bacterial genomes harbor phage-like elements or cryptic prophages. these elements have been implicated in pathogenesis, serotype conversion and phage immunity. the e14 element is a defective lambdoid prophage element present at 25 min in the e. coli k-12 genome. this prophage encodes important functional genes such as lit (t4 exclusion), mcra (modified cytosine restriction activity) and pin (recombinase).	2004	14733619
distant structural homology leads to the functional characterization of an archaeal pin domain as an exonuclease.	genome sequencing projects have focused attention on the problem of discovering the functions of protein domains that are widely distributed throughout living species but which are, as yet, largely uncharacterized. one such example is the pin domain, found in eukaryotes, bacteria, and archaea, and with suggested roles in signaling, rnase editing, and/or nucleotide binding. the first reported crystal structure of a pin domain (open reading frame pae2754, derived from the crenarchaeon, pyrobaculum ...	2004	14734548
effects of racing and nontraining on plasma thyroid hormone concentrations in sled dogs.	to determine the effects of racing and nontraining on plasma thyroxine (t4), free thyroxine (ft4), thyroid-stimulating hormone (tsh), and thyroglobulin autoantibody (tgaa) concentrations in sled dogs and compare results with reference ranges established for dogs of other breeds.	2004	14736066
do younger women with non-metastatic and non-inflammatory breast carcinoma have poor prognosis?	controversy abounds over whether breast cancer in younger women is more aggressive than those in older. the aim of the study was to assess the influence of age on long-term survival of women with breast carcinoma.	2004	14736343
the forces applied by female and male chiropractors during thoracic spinal manipulation.	on average, women weigh less, have a smaller frame, and are less muscular than men. since the peak thrust force applied during spinal manipulative treatments can be quite high and must be reached in a very short period of time, one might question the physical ability of women to generate such high forces.	2004	14739874
[malignomas of the nasal cavity and the paranasal sinuses: clinical characteristics, therapy and prognosis of different tumor types].	malignomas of the nasal cavity and the paranasal sinuses count for less than 3 % of the ent-malignancies.	2004	14740304
is helicobacter pylori infection a necessary condition for noncardia gastric cancer?	although the association between helicobacter pylori infection and gastric cancer is well established, this association might have been underestimated in epidemiologic studies because of possible clearance of the infection in the course of disease development. the authors addressed this hypothesis in a case-control study from saarland, germany (68 cases first diagnosed between 1996 and 1998 and 360 controls), with serologic assessment of h. pylori infection in which various exclusion criteria we ...	2004	14742285
effect of selenium depletion and supplementation on the kinetics of type i 5'-iodothyronine deiodinase and t3/t4 in rats.	presently, the effect of selenium (se) deficiency and excess of se (1 ppm) on the activity of selenoenzymes type 1 5'-iodothyronine deiodinase (5'-di), glutathione peroxidase (gsh-px), and level of thyroid hormones (t3 and t4) was studied in rats. se levels in the serum and liver, t3 and t4 in the serum, gsh-px levels in the liver, and 5'-di activity in the liver/aorta/thyroid were estimated after 1, 2, and 3 mo of se-deficient (0.02 ppm), se-adequate (0.2 ppm), and se-excess (1 ppm) diet feedin ...	2004	14742903
restoration of euthyroidism accelerates bone turnover in patients with subclinical hypothyroidism: a randomized controlled trial.	this study evaluated the effect of physiological l-thyroxine (l-t4) treatment on bone metabolism in patients with subclinical hypothyroidism. sixty-six women with subclinical hypothyroidism (tsh 11.7 +/- 0.8 miu/l) were randomly assigned to receive l-t4 or placebo for 48 weeks. sixty-one of 66 patients completed the study. individual l-t4 replacement (mean dosage 85.5 +/- 4.3 microg/day) was performed targeting euthyroid thyroid-stimulating hormone (tsh) levels. the primary outcome measure was 2 ...	2004	14727010
misplacement of a psoas compartment catheter in the subarachnoid space.	this case report describes an unusual cause of misplacement of an indwelling catheter in the subarachnoid space after primary psoas compartment block in a patient undergoing total knee arthroplasty.	2004	14727281
congenital hypothyroidism and apparent athyreosis with compound heterozygosity or compensated hypothyroidism with probable hemizygosity for inactivating mutations of the tsh receptor.	we wished to ascertain whether mutations in the tsh receptor (tshr) gene were present in two siblings with congenital hypothyroidism with no parental consanguinity.	2004	14725684
predictors of intellectual outcome in a cohort of brazilian children with congenital hypothyroidism.	to identify predictors of intellectual development in a cohort of children with permanent primary congenital hypothyroidism.	2004	14725688
evaluating the contribution of base stacking during translesion dna replication.	despite the nontemplating nature of the abasic site, damp is often preferentially inserted opposite the lesion, a phenomenon commonly referred to as the "a-rule". we have evaluated the molecular mechanism accounting for this unique behavior using a thorough kinetic approach to evaluate polymerization efficiency during translesion dna replication. using the bacteriophage t4 dna polymerase, we have measured the insertion of a series of modified nucleotides and have demonstrated that increasing the ...	2004	14717593
oncological outcomes after total mesorectal excision for cure for cancer of the lower rectum: anterior vs. abdominoperineal resection.	this study was designed to examine the outcome of cancer of the lower rectum, particularly the rates of local recurrence and survival for tumors located in this area that have been treated by anterior or abdominoperineal resections.	2004	14719151
morphometric comparison of the pedicle rib unit to pedicles in the thoracic spine.	a radiographic study of the pedicle rib unit morphology as compared with measurements of the pedicle in cadaveric thoracic spines.	2004	14722404
incidence of incisional recurrence after thoracoscopy.	incisional recurrence after thoracoscopic surgery has been reported infrequently. in recent years, several reports of port-site recurrence after laparoscopic oncologic procedures have been published. this study evaluates the incidence of incisional recurrence among patients with intrathoracic malignancy after diagnostic and therapeutic thoracoscopy.	2004	14716543
clinical and hormonal characteristics of central hypothyroidism at diagnosis and during follow-up in adult patients.	we studied the clinical and hormonal profiles of patients with central hypothyroidism (ch), the adequacy of levothyroxine (l-t4) treatment and the influence of other pituitary hormone replacement therapies.	2004	14713273
local staging of rectal cancer: assessment with double-contrast multislice computed tomography and transrectal ultrasound.	the aim of this study was to evaluate the accuracy of multislice computed tomography (msct) with double-contrast technique and transrectal ultrasound (trus) in staging of rectal carcinoma compared with histopathological confirmation.	2004	14716245
[prognostic significance of intrapulmonary metastases in cases of surgically treated lung cancer].	the aim of this retrospective study was to establish the prognosis in lung tumour cases in which resection was followed by synchronous or metachronous intrapulmonary metastasis.	2004	14716437
definitive chemoradiation in patients with inoperable oesophageal carcinoma.	we performed a retrospective study of 90 consecutive cases with inoperable carcinoma of the oesophagus treated with definitive chemoradiation at a single cancer centre between 1995 and 2002. for the last 4 years, 73 patients have received therapy according to an agreed protocol. this outpatient-based regimen involves four cycles of chemotherapy, cycles 3 and 4 given concurrently with 50 gy external beam radiotherapy (xrt) delivered in 25 fractions over 5 weeks. cisplatin 60 mg m(-2) day(-1) is g ...	2004	14710209
impact of nitrate intake in drinking water on the thyroid gland activity in male rat.	the purpose of this study was to determine the effects of nitrate on both the activity of the thyroid gland and other biological parameters. after 5-month treatment, nitrate 150 and 500 mg/l induced a significant decrease in the serum level of thyroid hormone t3. for t4, the 500 mg/l dose only reduced its plasma level. on the other hand, nitrate induced a dose-dependent increase in the weight of the thyroid gland. the histological study of the thyroid gland shows vacuolisation and an increase in ...	2004	14700525
factors that influence selection of coding resumption sites in translational bypassing: minimal conventional peptidyl-trna:mrna pairing can suffice.	this study investigates bypassing initiated from codons immediately 5' of a stop codon. the mrna slips and is scanned by the peptidyl-trna for a suitable landing site, and standard decoding resumes at the next 3' codon. this work shows that landing sites with potentially strong base pairing between the peptidyl-trna anticodon and mrna are preferred, but sites with little or no potential for watson-crick or wobble base pairing can also be utilized. these results have implications for re-pairing i ...	2004	14707145
cerebral cortical adaptations associated with visuomotor practice.	electroencephalographic (eeg) recordings were examined at the temporal (t3, t4) regions of the cerebral cortex in novice pistol shooters (n = 11) over a training period of 12-14 wk to determine changes in activation. mean alpha power and its rate of change were hypothesized to increase in the left temporal region during aiming from early to late season as participants improved their accuracy and reduced cognitive effort.	2004	14707777
permissive effects of thyroid hormones on rat anterior pituitary mitotic activity.	the anterior pituitary is active mitotically and apoptotically under basal conditions and in response to a variety of physiological and pathophysiological stimuli. hypothyroidism in man is associated with a modest but very occasionally dramatic increase in overall pituitary size. the mechanisms underlying this reversible phenomenon remain obscure. in the present study we have examined young adult rat anterior pituitary following surgical thyroidectomy and subsequent thyroid hormone treatment and ...	2004	14709142
endogenous thyroid hormones modulate pituitary somatotroph differentiation during chicken embryonic development.	growth hormone cell differentiation normally occurs between day 14 and day 16 of chicken embryonic development. we reported previously that corticosterone (cort) could induce somatotroph differentiation in vitro and in vivo and that thyroid hormones could act in combination with cort to further augment the abundance of somatotrophs in vitro. the objective of the present study was to test our hypothesis that endogenous thyroid hormones regulate the abundance of somatotrophs during chicken embryon ...	2004	14709143
rapid preparation of severe uncontrolled thyrotoxicosis due to graves' disease with iopanoic acid--a case report.	to report the rapid preoperative preparation of a case of thyrotoxicosis due to graves' disease resistant to medical treatment.	2004	14709458
predictive factors and distribution of lymph node metastasis in lip cancer patients and their implications on the treatment of the neck.	the frequency of neck metastasis in lip cancer patients is low, however if present, it decreases survival rates, which reinforce the neck treatment as an important step in the management of these patients. this study evaluates the predictive factors, the distribution of lymph node metastasis and their implications on the neck treatment. a retrospective analysis of lip cancer patients treated in our institution from 1969 to 1999 was performed. a total of 617 patients were analysed. one hundred an ...	2004	14693248
levobupivacaine for pediatric spinal anesthesia.	in this clinical trial we evaluated the clinical effects of levobupivacaine in spinal anesthesia in children. an open, noncomparative study was performed on 40 children, aged 1-14 yr, undergoing elective lower abdominal or lower limb surgery. a plain solution of s(-)-bupivacaine 5 mg/ml at a mean dose of 0.3 mg/kg body weight (range, 0.2-0.5 mg/kg body weight) was administered via the l3-4 or l4-5 interspace with the patient in the lateral decubitus position. after injection, the patients were p ...	2004	14693586
spinal 2-chloroprocaine: the effect of added fentanyl.	chloroprocaine is being investigated as a possible replacement for spinal lidocaine. adding fentanyl to lidocaine increases the quality of spinal anesthesia without prolongation of block. we report the characteristics of 2-chloroprocaine (2-cp) spinal anesthesia with or without fentanyl in 8 volunteers receiving 40 mg 2-cp with saline or 20 micro g fentanyl in a double-blinded, randomized, crossover manner. spinal anesthesia was successful for all subjects with complete block regression, ambulat ...	2004	14693593
spinal 2-chloroprocaine: the effect of added dextrose.	spinal 2-chloroprocaine is being investigated as an alternative short-acting spinal anesthetic to replace lidocaine for outpatient surgery. adding dextrose increases the baricity of solutions and alters the characteristics of spinal anesthesia. in this study, we compared 2-chloroprocaine spinal anesthesia performed with or without the addition of dextrose (1.1%). eight volunteers underwent 2 spinal anesthetics, receiving 40 mg 2-chloroprocaine (2 ml, 2%) with 0.25 ml saline with one and 0.25 ml ...	2004	14693594
quantitative and selective evaluation of differential sensory nerve block after transdermal lidocaine.	we evaluated the effect of transdermal lidocaine on differential sensory nerve block in 15 healthy volunteers. lidocaine 10% gel was applied topically to a forearm and covered with a plastic film. three types of sensory nerve fibers (abeta, adelta, and c fibers) were evaluated with a series of 2000-, 250-, and 5-hz stimuli using current perception threshold (cpt) testing. sensations of touch, pinprick, cold, and warmth were also measured. these measurements were made before the topical lidocaine ...	2004	14693629
na+,k+-atpase concentration and fiber type distribution after spinal cord injury.	complete spinal cord injury (sci) is characterized, in part, by reduced fatigue-resistance of the paralyzed skeletal muscle during stimulated contractions, but the underlying mechanisms are not fully understood. the effects of complete sci on skeletal muscle na(+),k(+)-adenosine triphosphatase (atpase) concentration, and fiber type distribution were therefore investigated. six individuals (aged 32.0 +/- 5.3 years) with complete paraplegia (t4-t10; 1-19 years since injury) participated. there was ...	2004	14694496
the c-terminal t peptide of acetylcholinesterase forms an alpha helix that supports homomeric and heteromeric interactions.	acetylcholinesterase subunits of type t (achet) possess an alternatively spliced c-terminal peptide (t peptide) which endows them with amphiphilic properties, the capacity to form various homo-oligomers and to associate, as a tetramer, with anchoring proteins containing a proline rich attachment domain (prad). the t peptide contains seven conserved aromatic residues. by spectroscopic analyses of the synthetic peptides covering part or all of the t peptide of torpedo achet, we show that the regio ...	2004	14686917
multimodal treatment strategies combining neoadjuvant hormonal therapy and/or chemotherapy with radical prostatectomy in high-risk localised prostate cancer.	by combining the readily available clinical parameters of tumour stage, gleason score of the diagnostic biopsy specimen and serum prostate-specific antigen level, men with newly diagnosed prostate cancer can be assessed as to their risk of treatment failure after radiotherapy or radical prostatectomy. for men considered to be at high-risk of treatment failure after local therapy alone, multimodal treatment strategies may result in improved cancer-control outcomes. this strategy has proven effect ...	2004	14680451
mandibular reconstruction: are two flaps better than one?	this study compared the combined iliac and ulnar forearm flaps with the osteomusculocutaneous fibular free flap for mandibular reconstruction. a retrospective study of 40 patients who had oromandibular reconstruction was performed, of whom 23 patients had a combined iliac crest without skin and ulnar forearm free flap. seventeen patients had an osteomusculocutaneous free fibular flap. ten women and 30 men with a mean age of 57.5 years comprised this study population. ninety percent of the cases ...	2004	14676696
ligase-mediated construction of branched dna strands: a novel dna joining activity catalyzed by t4 dna ligase.	branched nucleic acid strands exist as intermediates in certain biological reactions, and bifurcating dna also presents interesting opportunities in biotechnological applications. we describe here how t4 dna ligase can be used for efficient construction of dna molecules having one 5' end but two distinct 3' ends that extend from the 2' and 3' carbons, respectively, of an internal nucleotide. the nature of the reaction products is investigated, and optimal reaction conditions are reported for the ...	2004	14704359
improved full-length cdna production based on rna tagging by t4 dna ligase.	second-strand cdna priming is a central problem for full-length characterization of transcripts. a new strategy using bacteriophage t4 dna ligase and partially degenerate adapters is proposed for grafting a sequence tag to the end of polyribonucleotides. based on this rna tagging system and previously described protocols, a new method for full-length cdna production has been implemented. validation of the method is shown in arabidopsis thaliana by the construction of a full-length cdna library a ...	2004	14704363
recurrent rectal cancer within the pelvis. a multicenter analysis of 123 patients and recommendations for adjuvant radiotherapy.	recommendations for radiation ports in adjuvant radiation therapy for rectal cancer are mainly based on analysis of recurrence patterns. to evaluate whether changes in surgical technique have influenced this pattern of recurrence, a multicenter retrospective analysis was carried out on a patient population treated recently.	2004	14704840
loss of translational entropy in molecular associations.	molecular associations in solution are opposed by the loss of entropy (deltas) that results from the restriction of motion of each component in the complex. theoretical estimates of deltas are essential for rationalizing binding affinities, as well as for calculating entropic contribution to enzyme catalysis. recently a statistical-mechanical framework has been proposed for estimating efficiently the translational entropy loss (deltas(trsl)), while taking explicitly into account the complex inte ...	2004	14705027
repetitive transcranial magnetic stimulation improves open field locomotor recovery after low but not high thoracic spinal cord compression-injury in adult rats.	electromagnetic fields are able to promote axonal regeneration in vitro and in vivo. repetitive transcranial magnetic stimulation (rtms) is used routinely in neuropsychiatric conditions and as an atraumatic method to activate descending motor pathways. after spinal cord injury, these pathways are disconnected from the spinal locomotor generator, resulting in most of the functional deficit. we have applied daily 10 hz rtms for 8 weeks immediately after an incomplete high (t4-5; n = 5) or low (t10 ...	2004	14705146
doppler evaluation of the thyroid in pediatric goiter.	the aim of this study was to identify any changes in doppler parameters in the thyroid arteries of pediatric patients with a clinical diagnosis of goiter living in an area where goiter is endemic.	2004	14705174
multiple site-directed mutagenesis of more than 10 sites simultaneously and in a single round.	site-directed mutagenesis is a powerful tool to explore the structure-function relationship of proteins, but most traditional methods rely on the mutation of only one site at a time and efficiencies drop drastically when more than three sites are targeted simultaneously. many applications in functional proteomics and genetic engineering, including codon optimization for heterologous expression, generation of cysteine-less proteins, and alanine-scanning mutagenesis, would greatly benefit from a m ...	2004	14690693
effective scoring function for protein sequence design.	we have developed an effective scoring function for protein design. the atomic solvation parameters, together with the weights of energy terms, were optimized so that residues corresponding to the native sequence were predicted with low energy in the training set of 28 protein structures. the solvation energy of non-hydrogen-bonded hydrophilic atoms was considered separately and expressed in a nonlinear way. as a result, our scoring function predicted native residues as the most favorable in 59% ...	2004	14696189
separation of long dna molecules by quartz nanopillar chips under a direct current electric field.	we have established the nanofabrication technique for constructing nanopillars with high aspect ratio (100-500 nm diameter and 500-5000 nm tall) inside a microchannel on a quartz chip. the size of pillars and the spacing between pillars are designed as a dna sieving matrix for optimal analysis of large dna fragments over a few kilobase pairs (kbp). a chip with nanopillar channel and simple cross injector was developed based on the optimal design and applied to the separation of dna fragments (1- ...	2004	14697027
effects of iodothyronines on the hepatic outer-ring deiodinating pathway in killifish.	substrate availability has been thought to be a major regulator of the outer-ring deiodinating pathway (ord) in fish. however, current information strongly suggests that while fish iodothyronine deiodinase type 2 (d2) responds to iodothyronines in the same manner as its mammalian counterpart, fish deiodinase type 1 (d1) exhibits a distinct response. furthermore, 3,5-t2, generally considered to be an inactive product of iodothyronine metabolism, has recently been described as bioactive, but its e ...	2004	14697306
involvement of k+ channels and calcium-dependent pathways in the action of t3 on amino acid accumulation and membrane potential in sertoli cells of immature rat testis.	the purpose of this study was to investigate the involvement of calcium in k+ currents and its effects on amino acid accumulation and on the membrane potential regulated by tri-iodo-l-thyronine (t3) in sertoli cells. immature rat testes were pre-incubated for 30 min in krebs-ringer bicarbonate buffer and incubated for 60 min in the presence of [14c]methylaminoisobutyric acid with and without t3 or t4 (dose-response curve). specific channel blockers or chelating agents were added at different con ...	2004	14697410
nasopharyngeal carcinoma in children: ten years' experience at the tata memorial hospital, mumbai.	to evaluate the disease characteristics and outcome of children with nasopharyngeal carcinoma treated at the tata memorial hospital, mumbai.	2004	14697438
opposite effects of zinc and melatonin on thyroid hormones in rats.	the present study was conducted to investigate how thyroid function in rats is affected by administration of 3 mg per kg per day of zinc and/or melatonin. the study was conducted with 40 sprague-dawley adult male rats equally divided into four groups: 1 (controls), 2 (zinc-only), 3 (melatonin-only) and 4 (zinc- and melatonin-supplemented). the supplementation was continued for 4 weeks after which the animals were sacrificed and plasma samples were obtained for determination of zinc, melatonin, f ...	2004	14698569
influence of exogenous thyroxine on plasma melatonin in juvenile atlantic salmon (salmo salar).	one of the most clearly defined endocrine changes during the parr-smolt transformation of anadromous salmonids is an increase in plasma levels of thyroid hormones. the role of pineal hormone melatonin in timing and synchronisation of smoltification is widely discussed. the effect of administration of exogenous thyroxine (t4) on plasma melatonin was investigated in juvenile atlantic salmon (salmo salar) at the early stages of parr-smolt transformation. fish were kept in fresh water under simulate ...	2004	14698909
formation of highly stable chimeric trimers by fusion of an adenovirus fiber shaft fragment with the foldon domain of bacteriophage t4 fibritin.	the folding of beta-structured, fibrous proteins is a largely unexplored area. a class of such proteins is used by viruses as adhesins, and recent studies revealed novel beta-structured motifs for them. we have been studying the folding and assembly of adenovirus fibers that consist of a globular c-terminal domain, a central fibrous shaft, and an n-terminal part that attaches to the viral capsid. the globular c-terminal, or "head" domain, has been postulated to be necessary for the trimerization ...	2004	14699113
limited endoscopic thoracic sympathetic block for hyperhidrosis of the upper limb: reduction of compensatory sweating by clipping t4.	endoscopic thoracic sympathicotomy of t2 to t4 (ets 2-4) has evolved into an effective treatment for severe hyperhidrosis of the upper limb. complications such as bleeding or horner's syndrome are rare, but side effects such as compensatory and gustatory sweating occur in 30-50% of patients. following the lin-telaranta classification, we aimed to reduce these side-effects by clipping t4 solely [endoscopic thoracic sympathetic block (esb 4)]. we present our experience and clinical results using t ...	2004	14625754
role of his-16 in turnover of t4 pyrimidine dimer glycosylase.	previously, the histidine residue at position 16 in the mature t4 pyrimidine dimer glycosylase (t4-pdg) protein has been suggested to be involved in general (non-target) dna binding. this interpretation is likely correct, but, in and of itself, cannot account for the most dramatic phenotype of mutants at this position: their inability to restore ultraviolet light resistance to a dna repair-deficient escherichia coli strain. accordingly, this residue has been mutated to serine, glutamic, aspartic ...	2004	14610082
clinical, biochemical and therapeutical aspects of amiodarone-induced hypothyroidism (aih) in geriatric patients with cardiac arrhythmias.	the clinical features and the laboratory aspects of the amiodarone-induced hypothyroidism (aih) in the elderly as well as the effects of amiodarone treatment in aged aih people have not yet been well clarified. in the present paper, we evaluated 18 subjects of both sexes (7 females, 11 males), aged 65-83 years, affected by aih, recruited in central tuscany, italy. the patients were divided in two subsets on the basis of thyroid stimulating hormone (tsh) values: mild (tsh < 20 mu/l; group a, n=11 ...	2004	14599701
upper thoracic-spine disc degeneration in patients with cervical pain.	to study the relationship of upper thoracic spine degenerative disc contour changes on mr imaging in patients with neck pain. the relation between upper thoracic and cervical spine degenerative disc disease is not well established.	2004	14574518
early developmental neurotoxicity of a pcb/organochlorine mixture in rodents after gestational and lactational exposure.	the developmental and neurobehavioral effects of gestational and lactational exposure to a mixture of 14 polychlorinated biphenyls (pcbs) and 11 organochlorine pesticides was examined and compared against the commercial pcb mixture aroclor 1254. the mixture was based on blood levels reported in canadian populations living in the great lakes/st. lawrence basin. pregnant sprague-dawley rats were dosed orally with 0.013, 0.13, 1.3, or 13 mg/kg of the chemical mixture or 15 mg/kg of aroclor 1254 fro ...	2004	14514954
endocrine and metabolic effects of growth hormone (gh) compared with gh-releasing peptide, thyrotropin-releasing hormone, and insulin infusion in a rabbit model of prolonged critical illness.	treatment with recombinant human gh (rhgh) increases the mortality of critical illness. we postulated that combined gh-releasing peptide-2 (ghrp-2), trh, and insulin infusion is a less toxic anabolic strategy through a putative inability to overstimulate the gh axis and a capacity to normalize thyroid hormone concentrations while foregoing excessive hyperglycemia. burn-injured, parenterally fed, new zealand white rabbits were randomized to receive 4-d treatment with saline (n=8); 60 microg/kg.h ...	2004	14551231
expression of uridine diphosphate n-acetyl-alpha-d-galactosamine: polypeptide n-acetylgalactosaminyl transferase 3 in adenocarcinoma of the pancreas.	uridine diphosphate (udp) n-acetyl-alpha-d-galactosamine:polypeptide n-acetylgalactosaminyl transferase 3 (galnac-t3), one of the enzymes that catalyze the initial glycosylation of mucin type o-linked proteins, was shown to associate with the differentiation of cancer cell lines and the prognosis of some kinds of cancers. in the present study, the association of galnac-t3 expression with clinicopathologic features of pancreatic adenocarcinoma and patients' survival was examined. the level of exp ...	2004	14555840
association between expression levels of ca 19-9 and n-acetylglucosamine-beta;1,3-galactosyltransferase 5 gene in human pancreatic cancer tissue.	ca 19-9, equivalent to sialyl lewis antigen, is a well-known tumor marker in pancreatic cancer. at the initial step of the biosynthesis of ca 19-9, n-acetylglucosamine-beta1,3-galactosyltransferase (beta3gal-t) transfers galactose to n-acetylglucosamine (glcnac). recently, beta3gal-t5 has been presumed to be related to the formation of the type 1 chain in an in vitro experiment in terms of kinetic enzyme characterization. the purpose of this study was to investigate which beta3gal-t is related t ...	2004	14555842
the prognostic impact of metastatic pattern of lymph nodes in patients with oral and oropharyngeal squamous cell carcinomas.	in patients with squamous cell carcinomas of the oral cavity and the oropharynx the presence or absence of nodal metastases still is the most important predictive factor. the discriminative significance of extracapsular spread and the influence of features of the primary tumor-such as size and depth of invasion-on metastatic pattern, treatment failure and survival were evaluated. five-year postoperative follow-ups of 115 consecutively treated patients were studied retrospectively concerning the ...	2004	14504863
pelvic sidewall involvement in recurrent rectal cancer.	the lateral pelvic sidewall is an area not routinely dissected during standard operative procedures in surgery for rectal cancer in western countries. this study analyzed data to evaluate the pattern of recurrence in rectal cancer with special emphasis on lateral tumor extension in a recently treated patient population.	2004	14530995
dual activity of a viral lysozyme with high efficiency for growth inhibition of erwinia amylovora.	abstract the lysozyme from erwinia amylovora phage phiea1h was investigated for its ability to inhibit growth of bacteria and compared with the lysozyme from escherichia coli phage t4. the assays to measure lysozyme activity included cell lysis and growth inhibition of bacteria. bacterial strains with kanamycin resistance were not affected by lysates containing the phiea1h-enzyme. the titer of micrococcus luteus but not of erwinia amylovora was diminished by cell extracts containing t4 lysozyme. ...	2004	18943701
the effect of thyroid hormones in psoriasis vulgaris.	certain endocrinological disturbances are assumed to exacerbate psoriasis. in this study we compared the serum thyroid hormone levels of a group of psoriasis patients with that of a control group.	2004	17642662
the serum triiodothyronine to thyroxine (t3/t4) ratio in various thyroid disorders and after levothyroxine replacement therapy.	in order to examine the significance of differences in the triiodothyronine/thyroxine (t3/t4) ratio in the achievement of euthyroidism and in different thyroidal diseases, we studied 1050 subjects: 233 were euthyroid (eu), 239 hypothyroid (hypo) with initial tsh levels >15 mu/l, 273 hypothyroid on substitution therapy with l-thyroxine alone and tsh values of 0.35-3.5 mu/l, (hyporx), 236 hyperthyroid (hyper) and 69 in the acute phase of subacute thyroiditis de quervain's (dq). the ratio of t3/t4 ...	2004	16982586
clinical challenges and controversies in the management of hiv/ hcv-coinfected individuals.	the natural history of hiv infection has been dramatically changed by the highly active antiretroviral therapies, reducing complications, morbidity and mortality of the disease. approximately 25% of persons infected with hiv are co-infected with hepatitis c, and some high risk populations have a prevalence of hcv of more than 75%. liver disease has become one of the principal causes of morbidity and mortality in this population. co-infection increases viremia of hepatitis c, with increase in fib ...	2004	16929585
manipulation of dna through ce(iv)/edta-induced site-selective hydrolysis using phosphate-bearing oligonucleotides.	by using two oligonucleotides bearing a monophosphate group at the terminus and placing them at both edges of gap-site formed in substrate dna, this site was preferentially and efficiently hydrolyzed by ce(iv)/edta. in this study, site-selective hydrolysis of gap-site in long substrate dna (274-mer) was investigated. when two monophosphate groups were placed to both edges of 5-base gap, predetermined site was selectively hydrolyzed by ce(iv)/edta. furthermore, desired recombinant dna was prepare ...	2004	17150523
[weight gain of the predator podisus distinctus (heteroptera: pentatomidae) with combinations of the preys tenebrio molitor (coleoptera: tenebrionidae) and musca domestica (diptera: muscidae)].	little is known about podisus distinctus (stal) (heteroptera: pentatomidae) one of the asopinae species with good possibilities for mass rearing and releasing against defoliator caterpillars in eucalyptus reforested areas in brazil. we evaluated the impact of prey combinations on weight of nymphs and adults of p. distinctus. the prey were musca domestica l. (diptera: muscidae) and tenebrio molitor l. (coleoptera: tenebrionidae). the experiment was developed under 25 +/- 0.5 degrees c, 60 +/- 10% ...	2004	17357405
[effect of atracurium on pancuronium-induced neuromuscular block recovery.].	additional neuromuscular blocker doses are in general needed during wall closing after abdominal surgeries. this study aimed at determining during partial pancuronium-induced neuromuscular block recovery, the effect of additional atracurium dose on spontaneous neuromuscular block recovery.	2004	19471739