Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| safety evaluation of 1,4-alpha-glucan branching enzymes from bacillus stearothermophilus and aquifex aeolicus expressed in bacillus subtilis. | 1,4-alpha-glucan branching enzyme (be; ec 2.4.1.18) is a key biocatalyst in the synthesis of polysaccharides, and is therefore useful in the production of food ingredients. the bes evaluated in this study (be-01 and be-02) are obtained by fermentation of bacillus subtilis expressing the be gene from either bacillus stearothermophilus strain trbe14 or aquifex aeolicus strain vf5. the safety of be-01 and be-02 have not been previously evaluated, and therefore, both were subjected to standard toxic ... | 2009 | 19470400 |
| characterization of a mannose-6-phosphate isomerase from geobacillus thermodenitrificans that converts monosaccharides. | a recombinant mannose-6-phosphate isomerase from geobacillus thermodenitrificans (gtmpi) isomerizes aldose substrates possessing hydroxyl groups oriented in the same direction at the c2 and c3 positions such as the d- and l-forms of ribose, lyxose, talose, mannose, and allose. the activity of gtmpi for d-lyxose isomerization was optimal at ph 7.0, 70 degrees c and 1 mm co(2+). under these conditions, the k(cat) and k(m) values were 74,300 s(-1) and 390 mm for d-lyxose and 28,800 s(-1) and 470 mm ... | 2009 | 19390787 |
| geobacillus stearothermophilus 6-phosphogluconate dehydrogenase complexed with 6-phosphogluconate. | two crystal structures of recombinant geobacillus stearothermophilus 6-phosphogluconate dehydrogenase (gs6pdh) in complex with the substrate 6-phosphogluconate have been determined at medium resolution. gs6pdh shares significant sequence identity and structural similarity with the enzymes from lactococcus lactis, sheep liver and the protozoan parasite trypanosoma brucei, for which a range of structures have previously been reported. comparisons indicate that amino-acid sequence conservation is m ... | 2009 | 19407374 |
| versatile solidified nanofibrous cellulose-containing media for growth of extremophiles. | solidified media that employ a porous matrix of nanofibrous cellulose are described. the physicochemical stability of the porous structure allows the development of solidified media that can support the growth of extremophiles, such as acidophilic acidiphilium, alkaliphilic bacillus, thermophilic geobacillus and thermus, alkalithermophilic bacillus, and acidothermophilic sulfolobus microbes. the cellulose-supported media have several advantages over agar- and gellan gum-derived media, including ... | 2009 | 19411423 |
| robust experimental design for optimizing the microbial inhibitor test for penicillin detection in milk. | to use experimental design techniques and a multiple logistic regression model to optimize a microbiological inhibition test with dichotomous response for the detection of penicillin g in milk. | 2009 | 19413756 |
| allosteric regulation of the primase (dnag) activity by the clamp-loader (tau) in vitro. | during dna replication the helicase (dnab) recruits the primase (dnag) in the replisome to initiate the polymerization of new dna strands. dnab is attached to the tau subunit of the clamp-loader that loads the beta clamp and interconnects the core polymerases on the leading and lagging strands. the tau-dnab-dnag ternary complex is at the heart of the replisome and its function is likely to be modulated by a complex network of allosteric interactions. using a stable ternary complex comprising the ... | 2009 | 19415803 |
| models of single-molecule experiments with periodic perturbations reveal hidden dynamics in rna folding. | traditionally, microscopic fluctuations of molecules have been probed by measuring responses of an ensemble to perturbations. now, single-molecule experiments are capable of following fluctuations without introducing perturbations. however, dynamics not readily sampled at equilibrium should be accessible to nonequilibrium single-molecule measurements. in a recent study [qu, x. et al. proc. natl. acad. sci. u.s.a. 2008, 105, 6602-6607], the efficiency of fluorescence resonance energy transfer (fr ... | 2009 | 19415919 |
| the importance of an extra loop in the b-domain of an alpha-amylase from b. stearothermophilus us100. | to provide insight into the potential role of a loop in domain b of several bacterial alpha-amylases, molecular and structural investigation of bacillus stearothermophilus alpha-amylase (amy us100) was used as a model. combination deletion mutants of g(213), i(214) and g(215), described as a loop-forming on the surface bacterial amylases, were subjected to biochemical and structural investigation. thermoactivity, thermostability as well calcium requirement were studied for each mutant. thus, del ... | 2009 | 19422796 |
| application of reca and rpob sequence analysis on phylogeny and molecular identification of geobacillus species. | some geobacillus species have highly similar 16s rrna gene sequences, making 16s rdna sequence analysis-based identification problematic. to overcome this limitation, reca and rpob sequence analysis was evaluated as an alternative for distinguishing geobacillus species. | 2009 | 19426278 |
| an exopolysaccharide produced by geobacillus thermodenitrificans strain b3-72: antiviral activity on immunocompetent cells. | the immunomodulatory and antiviral effects of an extracellular polysaccharide (eps-2), produced by a strain of geobacillus thermodenitrificans isolated from a shallow marine vent of vulcano island (italy), were evaluated. in the present study, we show for the first time that eps-2 treatment hinder hsv-2 replication in human peripheral blood mononuclear cells (pbmc) but not in wish cells. in fact, high levels of ifn-alpha, il-12, ifn-gamma, tnf-alpha, il-18 were detected in supernatants of eps-2 ... | 2009 | 19428560 |
| cultivation characteristics of denitrification by thermophilic geobacillus sp. strain tdn01. | nine thermophilic denitrification bacteria were isolated from field soil, mud, and spa samples. the alignment of 16s rdna showed that all were identical to the genus geobacillus. two of the bacteria produced n2o and n2 gas and the other seven strains produced n2 gas from nitrate. we examined the growth substrates for geobacillus tdn01 and determined that sodium succinate, pyruvate, formate, acetate, glycerol, glucose, sucrose, and cellobiose well supported growth of the isolate. growth occurred ... | 2009 | 19436124 |
| interaction of if2 with the ribosomal gtpase-associated center during 70s initiation complex formation. | addition of an escherichia coli 50s subunit (50s(cy5)) containing a cy5-labeled l11 n-terminal domain (l11-ntd) within the gtpase-associated center (gac) to an e. coli 30s initiation complex (30sic(cy3)) containing cy3-labeled initiation factor 2 complexed with gtp leads to rapid development of a fret signal during formation of the 70s initiation complex (70sic). initiation factor 2 (if2) and elongation factor g (ef-g) induce similar changes in ribosome structure. here we show that such similari ... | 2009 | 19366171 |
| structural and mutational analyses of deinococcus radiodurans uvra2 provide insight into dna binding and damage recognition by uvras. | uvra proteins are key actors in dna damage repair and play an essential role in prokaryotic nucleotide excision repair (ner), a pathway that is unique in its ability to remove a broad spectrum of dna lesions. understanding the dna binding and damage recognition activities of the uvra family is a critical component for establishing the molecular basis of this process. here we report the structure of the class ii uvra2 from deinococcus radiodurans in two crystal forms. these structures, coupled wi ... | 2009 | 19368888 |
| [transglycosylation of benzo[h]quinazolines]. | this was the first study to apply cyclodextrin glucanotransferases (cgtase, ec 2.4.1.19) produced by mesophilic, thermophilic, alkaliphilic, and halophilic bacilli as well as pullulanase, beta-amylase, beta-galactosidase, and beta-fructofuranosidase for transglycosylation of benzo[h]quinazolines. the combination of cgtase produced by bacillus stearothermophilus st-88 and gamma-cyclodextrin (cd) used as a donor of glucosyl residues was the most efficient. the derivatives obtained are water-solubl ... | 2009 | 19382700 |
| [purification of recombinant dna methyltransferase m2.bstse from nickase-modification system nm.bstsei and study of the enzyme properties]. | the operon of nickase-modification system from bacillus stearothermophilus se-589 (recognition site 5'-gagtc-3') includes two dna methyltransferase genes: bstseim1 and bstseim2. gene encoding dna methyltransferase m2.bstsei was cloned in pjw vector and expressed in e. coli cells. the enzyme m2.bstsei has been isolated by chromatographic purification. m2.bstsei displays maximum activity at 55 degrees c and ph 7.5. the enzyme modifies adenine in dna sequence 5'-gagtc-3' and has substrate specifici ... | 2009 | 19334521 |
| isolation of alpha-glucosidase-producing thermophilic bacilli from hot springs of turkey. | from 42 different hot springs in 6 provinces belonging to distinct geographical regions of turkey, 451 thermophilic bacilli were isolated and 67 isolates with a high amylase activity were selected to determine the alpha-glucosidase production capacities by using pnpg as a substrate. alpha-glucosidase production capacities of the isolates varied within the range from 77.18 to 0.001 u/g. eleven of our thermophilic bacilli produced alpha-glucosidase at significant levels comparable with that of the ... | 2009 | 19334599 |
| simultaneous treatment of washing, disinfection and sterilization using ultrasonic levitation, silver electrolysis and ozone oxidation. | a new type of ultrasonic washer-disinfector-sterilizer, able to clean, disinfect and sterilize most kinds of reusable medical devices, has been developed by using the ultrasonic levitation function with umbrella-shape oscillators and ozone bubbling together with sterilization carried out by silver electrolysis. we have examined the biomedical and physicochemical performance of this instrument. prokariotic and gram-negative escherichia coli and eukariotic saccharomyces cerevisiae were killed by s ... | 2009 | 19344093 |
| discrimination between right and wrong purine dntps by dna polymerase i from bacillus stearothermophilus. | we used a series of datp and dgtp analogues to determine how dna polymerase i from bacillus stearothermophilus (bf), a prototypical a family polymerase, uses n-1, n(2), n-3, and n(6) of purine dntps to differentiate between right and wrong nucleotide incorporation. altering any of these nitrogens had two effects. first, it decreased the efficiency of correct incorporation of the resulting dntp analogue, with the loss of n-1 and n-3 having the most severe effects. second, it dramatically increase ... | 2009 | 19348507 |
| a novel endo-glucanase from the thermophilic bacterium geobacillus sp. 70pc53 with high activity and stability over a broad range of temperatures. | a thermophilic geobacillus bacterium secreting high activity of endo-glucanase (ec 3.2.1.4) was isolated from rice straw compost supplemented with pig manure. a full-length gene of 1,104 bp, cela, encoding this glycosyl hydrolase family 5 endo-glucanase of 368 amino acids was isolated. no related gene from geobacillus has been reported previously. the recombinant cela expressed in escherichia coli had an optimal activity at 65 degrees c and ph 5.0, and it exhibited tenfold greater specific activ ... | 2009 | 19296197 |
| construction of a gene knockout system for application in paenibacillus alvei ccm 2051t, exemplified by the s-layer glycan biosynthesis initiation enzyme wsfp. | the gram-positive bacterium paenibacillus alvei ccm 2051t is covered by an oblique surface layer (s-layer) composed of glycoprotein subunits. the s-layer o-glycan is a polymer of [-->3)-beta-d-galp-(1[alpha-d-glcp-(1-->6)]-->4)-beta-d-manpnac-(1-->] repeating units that is linked by an adaptor of -[groa-2-->opo2-->4-beta-d-manpnac-(1-->4)]-->3)-alpha-l-rhap-(1-->3)-alpha-l-rhap-(1-->3)-alpha-l-rhap-(1-->3)-beta-d-galp-(1--> to specific tyrosine residues of the s-layer protein. for elucidation of ... | 2009 | 19304819 |
| characterization of a novel thermostable carboxylesterase from geobacillus kaustophilus hta426 shows the existence of a new carboxylesterase family. | the gene gk3045 (741 bp) from geobacillus kaustophilus hta426 was cloned, sequenced, and overexpressed into escherichia coli rosetta (de3). the deduced protein was a 30-kda monomeric esterase with high homology to carboxylesterases from geobacillus thermoleovorans ny (99% identity) and geobacillus stearothermophilus (97% identity). this protein suffered a proteolytic cut in e. coli, and the problem was overcome by introducing a mutation in the gene (k212r) without affecting the activity. the res ... | 2009 | 19304850 |
| alkane inducible proteins in geobacillus thermoleovorans b23. | initial step of beta-oxidation is catalyzed by acyl-coa dehydrogenase in prokaryotes and mitochondria, while acyl-coa oxidase primarily functions in the peroxisomes of eukaryotes. oxidase reaction accompanies emission of toxic by-product reactive oxygen molecules including superoxide anion, and superoxide dismutase and catalase activities are essential to detoxify them in the peroxisomes. although there is an argument about whether primitive life was born and evolved under high temperature condi ... | 2009 | 19320977 |
| inactivation of geobacillus stearothermophilus in canned food and coconut milk samples by addition of enterocin as-48. | the cyclic bacteriocin enterocin as-48 was tested on a cocktail of two geobacillus stearothermophilus strains in canned food samples (corn and peas), and in coconut milk. as-48 (7 microg/g) reduced viable cell counts below detection levels in samples from canned corn and peas stored at 45 degrees c for 30 days. in coconut milk, bacterial inactivation by as-48 (1.75 microg/ml) was even faster. in all canned food and drink samples inoculated with intact g. stearothermophilus endospores, bacterioci ... | 2009 | 19269571 |
| rational design of bacillus stearothermophilus us100 l-arabinose isomerase: potential applications for d-tagatose production. | l-arabinose isomerases catalyze the bioconversion of d-galactose into d-tagatose. with the aim of producing an enzyme optimized for d-tagatose production, three bacillus stearothermophilus us100 l-arabinose isomerase mutants were constructed, purified and characterized. our results indicate that mutant q268k was significantly more acidotolerant and more stable at acidic ph than the wild-type enzyme. the n175h mutant has a broad optimal temperature range from 50 to 65 degrees c. with the aim of c ... | 2009 | 19278622 |
| structural and functional similarities between a ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco)-like protein from bacillus subtilis and photosynthetic rubisco. | the sequences classified as genes for various ribulose-1,5-bisphosphate (rubp) carboxylase/oxygenase (rubisco)-like proteins (rlps) are widely distributed among bacteria, archaea, and eukaryota. in the phylogenic tree constructed with these sequences, rubiscos and rlps are grouped into four separate clades, forms i-iv. in rubisco enzymes encoded by form i, ii, and iii sequences, 19 conserved amino acid residues are essential for co(2) fixation; however, 1-11 of these 19 residues are substituted ... | 2009 | 19279009 |
| hyperthermostable, ca(2+)-independent, and high maltose-forming alpha-amylase production by an extreme thermophile geobacillus thermoleovorans: whole cell immobilization. | the synthesis of extracellular alpha-amylase in geobacillus thermoleovorans was constitutive. the enzyme was secreted in metabolizable carbon sources as well as non-metabolizable synthetic analogues of glucose, but the titers were higher in the former than that in the latter. g. thermoleovorans is a fast-growing facultatively anaerobic bacterium that grows under both aerobic and anaerobic conditions and produces an extracellular amylolytic enzyme alpha-amylase with the by-product of lactic acid. ... | 2009 | 19280125 |
| independent saturation of three trprs subsites generates a partially assembled state similar to those observed in molecular simulations. | two new crystal structures of bacillus stearothermophilus tryptophanyl-trna synthetase (trprs) afford evidence that a closed interdomain hinge angle requires a covalent bond between amp and an occupant of either pyrophosphate or tryptophan subsite. they also are within experimental error of a cluster of structures observed in a nonequilibrium molecular dynamics simulation showing partial active-site assembly. further, the highest energy structure in a minimum action pathway computed by using ela ... | 2009 | 19174517 |
| two crystal structures of lysyl-trna synthetase from bacillus stearothermophilus in complex with lysyladenylate-like compounds: insights into the irreversible formation of the enzyme-bound adenylate of l-lysine hydroxamate. | aminoacyl-trna synthetase forms an enzyme-bound intermediate, aminoacyladenylate in the amino-acid activation reaction. this reaction is monitored by measuring the atp-ppi exchange reason in which [(32)p]ppi is incorporated into atp. we previously reported that l-lysine hydroxamate completely inhibited the l-lysine-dependent atp-ppi exchange reaction catalysed by lysyl-trna synthetase from bacillus stearothermophilus (bslysrs). several experiments suggested that bslysrs can adenylate l-lysine hy ... | 2009 | 19174549 |
| isolation and characterization of cellulose-degrading bacteria from the deep subsurface of the homestake gold mine, lead, south dakota, usa. | the present study investigated the cultivable mesophilic (37 degrees c) and thermophilic (60 degrees c) cellulose-degrading bacterial diversity in a weathered soil-like sample collected from the deep subsurface (1.5 km depth) of the homestake gold mine in lead, south dakota, usa. chemical characterization of the sample by x-ray fluorescence spectroscopy revealed a high amount of toxic heavy metals such as cu, cr, pb, ni, and zn. molecular community structures were determined by phylogenetic anal ... | 2009 | 19189143 |
| aeration control of thermophilic aerobic digestion using fluorescence monitoring. | the thermophilic aerobic digestion (tad) process is recognized as an effective method for rapid waste activated sludge (was) degradation and the deactivation of pathogenic microorganisms. yet, high energy costs due to heating and aeration have limited the commercialization of economical tad processes. previous research on autothermal thermophilic aerobic digestion (atad) has already reduced the heating cost. however, only a few studies have focused on reducing the aeration cost. therefore, this ... | 2009 | 19190414 |
| phylogenetic, inter, and intraspecific sequence analysis of spo0a gene of the genus geobacillus. | in this work, the variability of spo0a gene in the genus geobacillus and applicability of this gene for the taxonomy within this genus were evaluated. the protein spo0a is the master regulator of the endospore-forming process in the all endospore-forming bacteria. geobacillus genus-specific primers geospo were designed based on the sequences of geobacillus spo0a gene available through the public databases. inter and intraspecific variability of geobacillus spo0a gene was determined after sequenc ... | 2009 | 19205799 |
| rna and protein complexes of trp rna-binding attenuation protein characterized by mass spectrometry. | we have characterized both wild-type and mutant trap (trp rna-binding attenuation protein) from bacillus stearothermophilus , and their complexes with rna or its regulator anti-trap protein (at), by electrospray ionization mass spectrometry (esi-ms). wild-type trap mainly forms homo-11mer rings. the mutant used carries three copies of the trap monomer on a single polypeptide chain so that it associates to form a 12mer ring with four polypeptide molecules. mass spectra showed that both the wild-t ... | 2009 | 19219981 |
| gene cloning, expression, and characterization of the geobacillus thermoleovorans ccr11 thermoalkaliphilic lipase. | the gene for a geobacillus thermoleovorans ccr11 thermostable lipase was recovered by pcr and cloned. four genetic constructions were designed and successfully expressed in e. coli: (i) the lipase structural gene (lipccr11) in the pinpoint xa vector; (ii) the lipase structural gene (lipaccr11) in the pet-28a(+) vector; (iii) the lipase structural gene minus the signal peptide (lipmatccr11) in the pet-3b vector; and (iv) the lipase structural gene plus its own promoter (lipproccr11) in the pgem-t ... | 2009 | 19107605 |
| molecular dynamics study of the structure, flexibility and dynamics of thermostable l1 lipase at high temperatures. | molecular dynamics (md) simulations have been used to understand how protein structure, dynamics, and flexibility are affected by adaptation to high temperature for several years. we report here the results of the high temperature md simulations of bacillus stearothermophilus l1 (l1 lipase). we found that the n-terminal moiety of the enzyme showed a high flexibility and dynamics during high temperature simulations which preceded and followed by clear structural changes in two specific regions; t ... | 2009 | 19130194 |
| kinetics of deoxy-ctp incorporation opposite a dg-c8-n-2-aminofluorene adduct by a high-fidelity dna polymerase. | the model carcinogen n-2-acetylaminofluorene covalently binds to the c8 position of guanine to form two adducts, the n-(2'-deoxyguanosine-8-yl)-aminofluorene (g-af) and the n-2-(2'-deoxyguanosine-8-yl)-acetylaminofluorene (g-aaf). although they are chemically closely related, their biological effects are strongly different and they are processed by different damage tolerance pathways. g-af is bypassed by replicative and high-fidelity polymerases, while specialized polymerases ensure synthesis pa ... | 2009 | 19150355 |
| cloning, purification and characterization of geobacillus stearothermophilus v uroporphyrinogen-iii c-methyltransferase: evaluation of its role in resistance to potassium tellurite in escherichia coli. | the geobacillus stearothermophilus v coba gene encoding uroporphyrinogen-iii c-methyltransferase (also referred to as sumt) was cloned into escherichia coli and the recombinant enzyme was overexpressed and purified to homogeneity. the enzyme binds s-adenosyl-l-methionine and catalyzes the production of iii methyl uroporphyrinogen in vitro. e. coli cells expressing the g. stearothermophilus v coba gene exhibited increased resistance to potassium tellurite and potassium tellurate. site-directed mu ... | 2009 | 19154787 |
| immobilization of recombinant thermostable beta-galactosidase from bacillus stearothermophilus for lactose hydrolysis in milk. | a recombinant thermostable beta-galactosidase from bacillus stearothermophilus was immobilized onto chitosan using tris(hydroxymethyl)phosphine (thp) and glutaraldehyde, and a packed bed reactor was utilized to hydrolyze lactose in milk. the thermostability and enzyme activity of thp-immobilized beta-galactosidase during storage was superior to that of free and glutaraldehyde-immobilized enzymes. the thp-immobilized beta-galactosidase showed greater relative activity in the presence of ca(2+) th ... | 2009 | 19164659 |
| characterization of bacillus stearothermophilus infa and of its product if1. | bacillus stearothermophilus infa encoding translation initiation factor if1 was cloned and expressed in escherichia coli and its transcript and protein product characterized. although the functional properties of b. stearothermophilus and e. coli if1, compared in several translational tests in the presence of both homologous and heterologous components, are not entirely identical, the two proteins are interchangeable in an in vitro translational system programmed with a natural mrna. the availab ... | 2009 | 18951960 |
| engineering of the alpha-amylase from geobacillus stearothermophilus us100 for detergent incorporation. | amyus100deltaig is a variant of the most thermoactive and thermostable maltohexaose forming alpha-amylase produced by geobacillus stearothermophilus sp.us100. this enzyme which was designed to improve the thermostability of the wild-type enzyme has acquired a very high resistance to chelator agents. according to modeling structural studies and with the aim of enhancing its resistance towards chemical oxidation, a mutant (amyus100deltaig/m197a) was created by substituting methionine 197 to alanin ... | 2009 | 18951544 |
| purification and characterization of a glycoside hydrolase family 43 beta-xylosidase from geobacillus thermoleovorans it-08. | the gene encoding a glycoside hydrolase family 43 beta-xylosidase (gbtxyl43a) from the thermophilic bacterium geobacillus thermoleovorans strain it-08 was synthesized and cloned with a c-terminal his-tag into a pet29b expression vector. the recombinant gene product termed gbtxyl43a was expressed in escherichia coli and purified to apparent homogeneity. michaelis-menten kinetic parameters were obtained for the artificial substrates p-nitrophenyl-beta-d: -xylopyranose (4npx) and p-nitrophenyl-alph ... | 2009 | 18815904 |
| characterization and identification of geobacillus spp. isolated from soldhar hot spring site of garhwal himalaya, india. | 13 morphologically distinct strains of thermophilic bacteria isolated from a hot spring site in garhwal region of indian himalaya have been characterized and identified using phenotypic and genotypic characters. all the strains developed circular to irregular colonies between 2-3 mm on tryptone yeast extract (ty) agar plates at 65 degrees c following 24-36 h incubation. in ty broth, facultative bacterial growth was observed within 12-16 h of incubation at 65 degrees c. the bacterial strains coul ... | 2009 | 19025872 |
| structural and functional studies of bacillus stearothermophilus serine hydroxymethyltransferase: the role of asn(341), tyr(60) and phe(351) in tetrahydrofolate binding. | shmt (serine hydoxymethyltransferase), a type i pyridoxal 5'-phosphate-dependent enzyme, catalyses the conversion of l-serine and thf (tetrahydrofolate) into glycine and 5,10-methylene thf. shmt also catalyses several thf-independent side reactions such as cleavage of beta-hydroxy amino acids, transamination, racemization and decarboxylation. in the present study, the residues asn(341), tyr(60) and phe(351), which are likely to influence thf binding, were mutated to alanine, alanine and glycine ... | 2009 | 19046138 |
| thermodynamic analysis reveals a temperature-dependent change in the catalytic mechanism of bacillus stearothermophilus tyrosyl-trna synthetase. | catalysis of trna(tyr) aminoacylation by tyrosyl-trna synthetase can be divided into two steps. in the first step, tyrosine is activated by atp to form the tyrosyl-adenylate intermediate. in the second step, the tyrosyl moiety is transferred to the 3' end of trna. to investigate the roles that enthalpic and entropic contributions play in catalysis by bacillus stearothermophilus tyrosyl-trna synthetase (tyrrs), the temperature dependence for the activation of tyrosine and subsequent transfer to t ... | 2009 | 19098308 |
| evaluation of a microbiological multi-residue system on the detection of antibacterial substances in ewe milk. | to protect both, public health and the dairy industry, from the presence of antibiotic residues in milk, control programmes have been established, which include the needed screening tests. this work focuses on the application of a microbiological multi-residue system in ewe milk, a method based on the use of six different plates, each seeded with one of the following bacteria: geobacillus stearothermophilus var. calidolactis (beta-lactams), bacillus subtilis at ph 8.0 (aminoglycosides), kocuria ... | 2009 | 19100896 |
| how to switch off a histidine kinase: crystal structure of geobacillus stearothermophilus kinb with the inhibitor sda. | entry to sporulation in bacilli is governed by a histidine kinase phosphorelay, a variation of the predominant signal transduction mechanism in prokaryotes. sda directly inhibits sporulation histidine kinases in response to dna damage and replication defects. we determined a 2.0-a-resolution x-ray crystal structure of the intact cytoplasmic catalytic core [comprising the dimerization and histidine phosphotransfer domain (dhp domain), connected to the atp binding catalytic domain] of the geobacil ... | 2009 | 19101565 |
| engineering a reversible, high-affinity system for efficient protein purification based on the cohesin-dockerin interaction. | efficient degradation of cellulose by the anaerobic thermophilic bacterium, clostridium thermocellum, is carried out by the multi-enzyme cellulosome complex. the enzymes on the complex are attached in a calcium-dependent manner via their dockerin (doc) module to a cohesin (coh) module of the cellulosomal scaffoldin subunit. in this study, we have optimized the coh-doc interaction for the purpose of protein affinity purification. a c. thermocellum coh module was thus fused to a carbohydrate-bindi ... | 2009 | 18979459 |
| comparison studies of the human heart and bacillus stearothermophilus lactate dehydrogreanse by transition path sampling. | transition path sampling is a well-known technique that generates reactive paths ensembles. due to the atomic detail of these reactive paths, information about chemical mechanisms can be obtained. we present here a comparative study of bacillus stearothermophilus and human heart homologues of lactate dehydrogenase (ldh). a comparison of the transition path ensemble of both enzymes revealed that small differences in the active site reverses the order of the particle transfer of the chemical step. ... | 2009 | 19053545 |
| identification of new secreted proteins and secretion of heterologous amylase by c. glutamicum. | in this study, secreted corynebacterium glutamicum proteins were investigated by two-dimensional gel electrophoresis. around 100 spots observed in the ph range 4.5-5.5 had molecular masses that varied from 10 to 50 kda. upon n-terminal amino acid sequence analysis by edman degradation, two of them were hits to two hypothetical proteins encoded by cgr_1176 and cgr_2070 on c. glutamicum r genome, respectively. active-form alpha-amylase derived from geobacillus stearothermophilus was successfully s ... | 2009 | 19066885 |
| screening and distributing features of bacteria with hydantoinase and carbamoylase. | hydantoinase and carbamoylase are key biocatalysts for the production of optically pure amino acids from dl-5-substituted hydantoins (ssh). out of 364 isolated strains with hydantoinase and carbamoylase at 45 degrees c, 24 strains showed efficient hydantoinase and carbamoylase activities. among them, 17 produced l-amino acids, and 7 produced d-amino acids from both aromatic dl-5-benzylhydantoin and aliphatic dl-5-isopropylhydantoin. most of the strains that were able to form l-amino acid belonge ... | 2009 | 17498938 |
| the microbial signature of aerosols produced during the thermophilic phase of composting. | the microbial diversity of bioaerosols released during operational activities at composting plants is poorly understood. identification of bacteria and fungi present in such aerosols is the prerequisite for the definition of microbial indicators that could be used in dispersal and exposure studies. | 2010 | 19602015 |
| reclassification of geobacillus pallidus (scholz et al. 1988) banat et al. 2004 as aeribacillus pallidus gen. nov., comb. nov. | although anoxybacillus and geobacillus, two genera of thermophilic bacteria close to the genus bacillus, have only been described recently, the number of species in these genera has increased rapidly. four thermophilic, lipolytic strains (dr01, dr02, dr03 and dr04) isolated from a hot spring in veracruz (mexico), which could not be identified phenotypically, were subjected to 16s rrna gene sequence analysis. three strains were identified as belonging to the genus anoxybacillus, but strain dr03 w ... | 2010 | 19700455 |
| the n-terminal region is crucial for the thermostability of the g-domain of bacillus stearothermophilus ef-tu. | bacterial elongation factor tu (ef-tu) is a model monomeric g protein composed of three covalently linked domains. previously, we evaluated the contributions of individual domains to the thermostability of ef-tu from the thermophilic bacterium bacillus stearothermophilus. we showed that domain 1 (g-domain) sets up the basal level of thermostability for the whole protein. here we chose to locate the thermostability determinants distinguishing the thermophilic domain 1 from a mesophilic domain 1. ... | 2010 | 19800034 |
| characterization of thermostable cellulases produced by bacillus and geobacillus strains. | the composition of thermophilic (60 degrees c) mixed cellulose-degrading enrichment culture initiated from compost samples was examined by constructing a 16s rrna gene clone library and the presence of sequences related to actinobacteria, bacteroidetes, chloroflexi, deinococcus-thermus, firmicutes, and proteobacteria were identified. eight isolates capable of degrading cellulose, carboxymethyl cellulose (cmc), or ponderosa pine sawdust were identified as belonging to the genera geobacillus, ther ... | 2010 | 20599378 |
| cloning and characterization of a xylanase, krict px1 from the strain paenibacillus sp. hpl-001. | the krict px1 gene (gb: fj380951) consisting of 996bp encoding a protein of 332 amino acids (38.1kda) from the recently isolated paenibacillus sp. strain hpl-001 (kctc11365bp) has been cloned and expressed in escherichia coli. the xylanase krict px1 showed high activity on birchwood xylan, and was active over a ph range of 5.0 to 11.0, with two optima at ph 5.5 and 9.5 at 50 degrees c with k(m) value of 5.35 and 3.23, respectively. the xylanase activity was not affected by most salts, such as na ... | 2010 | 20493247 |
| characterization of spore surfaces from a geobacillus sp. isolate by ph dependence of surface charge and infrared spectra. | the surfaces of spores from a geobacillus sp. isolated from a milk powder production line were examined to obtain fundamental information relevant to bacterial spore adhesion to materials. | 2010 | 20522147 |
| temperature dependence of protein motions in a thermophilic dihydrofolate reductase and its relationship to catalytic efficiency. | we report hydrogen deuterium exchange by mass spectrometry (hdx-ms) as a function of temperature in a thermophilic dihydrofolate reductase from bacillus stearothermophilus (bs-dhfr). protein stability, probed with circular dichroism, established an accessible temperature range of 10 degrees c to 55 degrees c for the interrogation of hdx-ms. although both the rate and extent of hdx are sensitive to temperature, the majority of peptides showed rapid kinetics of exchange, allowing us to focus on pl ... | 2010 | 20534574 |
| a study on the incidence, microbiological analysis and investigations on the source of infection of postoperative infectious endophthalmitis in a tertiary care ophthalmic hospital: an 8-year study. | the objective of the study was the determination of the incidence of culture-proven postoperative endophthalmitis and probable sources of infection. | 2010 | 20534919 |
| proanthocyanidins from the leaves of machilus philippinensis. | seven proanthocyanidins (2-8) together with epicatechin (1) were isolated from the etoh extract of the leaves of machilus philippinensis. of these, machiphilitannins a (7) and b (8) are new natural products, with respective ic(50) values of 31.3 (7) and 18.4 microm (8) against alpha-glucosidase type iv from bacillus stearothermophilus. their structures were elucidated mainly on the basis of cd and 2d nmr analyses. in addition, aesculitannin b (2) showed inhibitory activity against alpha-glucosid ... | 2010 | 20568785 |
| a novel thermostable carboxylesterase from geobacillus thermodenitrificans: evidence for a new carboxylesterase family. | a novel gene encoding an esterase from geobacillus thermodenitrificans strain cmb-a2 was cloned, sequenced and functionally expressed in escherichia coli m15. sequence analysis revealed an open reading frame of 747 bp corresponding to a polypeptide of 249 amino acid residues (named estgta2). after purification, a specific activity of 2.58 u mg(-1) was detected using p-np caprylate (c8) at 50 degrees c and ph 8.0 (optimal conditions). the enzyme catalyses the hydrolysis of triglycerides (tributyr ... | 2010 | 20587647 |
| evolution of a single gene highlights the complexity underlying molecular descriptions of fitness. | evolution by natural selection is the driving force behind the endless variation we see in nature, yet our understanding of how changes at the molecular level give rise to different phenotypes and altered fitness at the population level remains inadequate. the reproductive fitness of an organism is the most basic metric that describes the chance that an organism will succeed or fail in its environment and it depends upon a complex network of inter- and intramolecular interactions. a deeper under ... | 2010 | 20590336 |
| pcr detection of thermophilic spore-forming bacteria involved in canned food spoilage. | thermophilic bacteria that form highly heat-resistant spores constitute an important group of spoilage bacteria of low-acid canned food. a pcr assay was developed in order to rapidly trace these bacteria. three pcr primer pairs were designed from rrna gene sequences. these primers were evaluated for the specificity and the sensitivity of detection. two primer pairs allowed detection at the species level of geobacillus stearothermophilus and moorella thermoacetica/thermoautrophica. the other pair ... | 2010 | 20397018 |
| enhanced production of lipase by the thermophilic geobacillus stearothermophilus strain-5 using statistical experimental designs. | statistically based experimental designs were applied to optimize the cultural conditions for the production of a glycerol-inducible lipase from the thermophilic geobacillus stearothermophilus strain-5. the effect of nineteen culture conditions on enzyme production was evaluated using plackett-burman factorial design. tween 80, k(2)hpo(4), glycerol and glucose were the most significant factors in improving enzyme production. the selected parameters were then further investigated using central co ... | 2010 | 20412872 |
| a selection that reports on protein-protein interactions within a thermophilic bacterium. | many proteins can be split into fragments that exhibit enhanced function upon fusion to interacting proteins. while this strategy has been widely used to create protein-fragment complementation assays (pcas) for discovering protein-protein interactions within mesophilic organisms, similar assays have not yet been developed for studying natural and engineered protein complexes at the temperatures where thermophilic microbes grow. we describe the development of a selection for protein-protein inte ... | 2010 | 20418388 |
| detection of fusarium graminearum dna using a loop-mediated isothermal amplification (lamp) assay. | loop-mediated isothermal amplification (lamp) of dna is a simple, cost effective, and rapid method for the specific detection of genomic dna using a set of six oligonucleotide primers with eight binding sites hybridizing specifically to different regions of a target gene, and a thermophilic dna polymerase from geobacillus stearothermophilus for dna amplification. the method has been applied in various assays for the diagnosis of bacterial and viral infections of humans and animals, sexing of bov ... | 2010 | 20442002 |
| molecular dynamics studies on t1 lipase: insight into a double-flap mechanism. | t1 lipase is isolated from the palm geobacillus zalihae strain t1 in malaysia, functioning as a secreted protein responsible for the catalyzing hydrolysis of long-chain triglycerides into fatty acids and glycerol at high temperatures. in the current study, using 30 ns molecular dynamics simulations at different temperatures, an aqueous activation was detected for t1 lipase. this aqueous activation in t1 lipase was mainly caused by a double-flap movement mechanism. the double flaps were constitut ... | 2010 | 20443585 |
| physicochemical characteristics of a thermostable gellan lyase from geobacillus stearothermophilus 98. | a purified thermostable gellan lyase, produced by a thermophilic bacterium, geobacillus stearothermophilus 98, was characterized in relation to its physicochemical properties. the gellan lyase was established to have a molecular weight of 216 kda, defined by capillary gel electrophoresis. amino acid analysis revealed high quantities of lys, his, ala, val, ile, glx, and pro residues. the circular dichroism revealed 45% beta-structure and practically lack of a-spiral domains. kinetic studies showe ... | 2010 | 20469643 |
| thermostable l-arabinose isomerase from bacillus stearothermophilus iam 11001 for d-tagatose production: gene cloning, purification and characterisation. | d-tagatose, as one of the rare sugars, has been found to be a natural and safe low-calorie sweetener in food products and is classified as a gras substance. l-arabinose isomerase (l-ai, ec 5.3.1.4), catalysing the isomerisations of l-arabinose and d-galactose to l-ribulose and d-tagatose respectively, is considered to be the most promising enzyme for the production of d-tagatose. | 2010 | 20474051 |
| improved yields of cyclic nigerosylnigerose from starch by pretreatment with a thermostable branching enzyme. | cyclic nigerosylnigerose (cnn) is produced enzymatically from starch by the combined action of 6-alpha-glucosyltransferase and 3-alpha-isomaltosyltransferase. in our previous study, alpha-1,6-branching chains found in the structure of amylopectin and glycogen were shown to be favorable for cnn formation by the two enzymes. therefore, we examined whether the introduction of alpha-1,6-branch points into starch using the action of branching enzyme (be) could improve the yield of cnn from starch. th ... | 2010 | 20226381 |
| substrate specificity and biochemical properties of m3.bstf5i dna methyltransferase from the bstf5i restriction-modification system. | optimal conditions for dna methylation by the m3.bstf5i enzyme from bacillus stearothermophilus and kinetic parameters of lambda phage dna modification and that of a number of oligonucleotide substrates are established. comparison of m1.bstf5i and m3.bstf5i kinetic parameters revealed that with similar temperature optima and affinity for dna, m3.bstf5i has nearly fourfold lower turnover number (0.24 min(-1)) and modifies the hemimethylated recognition site with lower efficiency under optimal con ... | 2010 | 20331425 |
| structural study of carboxylesterase from hyperthermophilic bacteria geobacillus stearothermophilus by molecular dynamics simulation. | carboxylesterases are ubiquitous enzymes with important physiological, industrial and medical applications such as synthesis and hydrolysis of stereo specific compounds, including the metabolic processing of drugs, and antimicrobial agents. here, we have performed molecular dynamics simulations of carboxylesterase from hyperthermophilic bacterium geobacillus stearothermophilus (gsest) for 10ns each at five different temperatures namely at 300k, 343k, 373k, 473k and 500k. profiles of root mean sq ... | 2010 | 20347362 |
| preparation of carboxylated magnetic particles for the efficient immobilization of c-terminally lysine-tagged bacillus stearothermophilus aminopeptidase ii. | this article reports the synthesis and use of surface-modified iron oxide particles for the simultaneous purification and immobilization of bacillus stearothermophilus aminopeptidase ii (bsapii) tagged c-terminally with either tri- or nona-lysines (bsapii-lys(3/9)). the carboxylated magnetic particles were prepared by the simple co-precipitation of fe(3+)/fe(2+) in aqueous medium and then subsequently modified with adipic acid. transmission electron microscopy (tem) micrographs showed that the c ... | 2010 | 20373125 |
| enzymatic synthesis of glycosylated puerarin using maltogenic amylase from bacillus stearothermophilus expressed in bacillus subtilis. | the maltogenic amylase from bacillus stearothermophilus (bsma) is a valuable biocatalyst that has been used to transglycosylate natural glycosides to improve solubility. to ensure safety, bsma was produced in bacillus subtilis, using new shuttle vector-based expression vectors. the transglycosylation of puerarin was also conducted with crude bsma and analyzed. | 2010 | 20393999 |
| comparison of the effectiveness of sterilizing endodontic files by 4 different methods: an in vitro study. | sterilization is the best method to counter the threats of microorganisms. the purpose of sterilization in the field of health care is to prevent the spread of infectious diseases. in dentistry, it primarily relates to processing reusable instruments to prevent cross-infection. the aim of this study was to investigate the efficacy of 4 methods of sterilizing endodontic instruments: autoclaving, carbon dioxide laser sterilization, chemical sterilization (with glutaraldehyde) and glass-bead steril ... | 2010 | 20215664 |
| studying subunit-subunit interactions in a bacterial abc transporterby in vitro assembly. | the thermostable arginine abc transporter of geobacillus stearothermophilus consists of a solute binding protein, artj; a transmembrane subunit, artm; and a nucleotide-binding subunit, artp. an artm/his(6)-artp complex was functionally assembled from separately purified subunits as demonstrated by assaying stimulation of its atpase activity by arginine-loaded artj in proteoliposomes. studying in vitro assembly with variants carrying mutations in the conserved q loop and/or the eaa loop of artp a ... | 2010 | 20226162 |
| geobacillus sp., a thermophilic soil bacterium producing volatile antibiotics. | geobacillus, a bacterial genus, is represented by over 25 species of gram-positive isolates from various man-made and natural thermophilic areas around the world. an isolate of this genus (m-7) has been acquired from a thermal area near yellowstone national park, mt and partially characterized. the cells of this organism are globose (ca. 0.5 mu diameter), and they are covered in a matrix capsule which gives rise to elongate multicelled bacilliform structures (ranging from 3 to 12 mum) as seen by ... | 2010 | 20091406 |
| alkaline ph-dependent differential unfolding characteristics of mesophilic and thermophilic homologs of dimeric serine hydroxymethyltransferase. | environmental variables such as ph can significantly influence the folding and stability of a protein molecule. in the present investigation, we compared the alkaline ph-induced unfolding of two homologous serine hydroxymethyltransferase from mesophilic bacillus subtilis (bsshmt) and thermophilic bacillus stearothermophilus (bstshmt) using various biophysical techniques. the thermophilic enzyme bstshmt was found to be more resistant to alkaline denaturation compared to its mesophilic counterpart ... | 2010 | 20152942 |
| the second extracellular loop of pore-forming subunits of atp-binding cassette transporters for basic amino acids plays a crucial role in interaction with the cognate solute binding protein(s). | in the thermophile geobacillus stearothermophilus, the uptake of basic amino acids is mediated by an abc transporter composed of the substrate binding protein (receptor) artj and a homodimer each of the pore-forming subunit, artm, and the nucleotide-binding subunit, artp. we recently identified two putative binding sites in artj that might interact with the art(mp)(2) complex, thereby initiating the transport cycle (a. vahedi-faridi et al., j. mol. biol. 375:448-459, 2008). here we investigated ... | 2010 | 20154136 |
| characterization of a broad-range aldehyde dehydrogenase involved in alkane degradation in geobacillus thermodenitrificans ng80-2. | an aldehyde dehydrogenase (aldh) involved in alkane degradation in crude oil-degrading geobacillus thermodenitrificans ng80-2 was characterized in vitro. the aldh was expressed heterologously in escherichia coli and purified as a his-tagged homotetrameric protein with a subunit of 57 kda based on sds-page and native-page analysis. the purified aldh-oxidized alkyl aldehydes ranging from formaldehyde (cā) to eicosanoic aldehyde (cāā) with the highest activity on cā. it also oxidized several aromat ... | 2010 | 20171064 |
| the structure of dinb from geobacillus stearothermophilus: a representative of a unique four-helix-bundle superfamily. | the crystal structure of the dinb gene product from geobacillus stearothermophilus (gsdinb) is reported at 2.5 a resolution. the dinb gene is one of the dna-damage-induced genes and the corresponding protein, dinb, is the founding member of a pfam family with no known function. the protein contains a four-helix up-down-down-up bundle that has previously been described in the literature in three disparate proteins: the enzyme mdmpi (mycothiol-dependent maleylpyruvate isomerase), yfit and ttha0303 ... | 2010 | 20208147 |
| refolding of the non-specific neutral protease from bacillus stearothermophilus proceeds via an autoproteolytically sensitive intermediate. | a very thermostable variant of the thermolysin-like protease from bacillus stearothermophilus (g8c/n60c) was previously created by introduction of a disulfide bond into the cysteine-free pseudo-wild type variant (pwt) and thus fixing the unfolding region 56-69. in the present paper, we show that g8c/n60c and pwt can be reactivated from the completely unfolded states, accessible at >or=7.5m guanidine hydrochloride, and analyze the kinetics of folding, autoproteolytic degradation and aggregation. ... | 2010 | 20096501 |
| structural basis of substrate binding in wsaf, a rhamnosyltransferase from geobacillus stearothermophilus. | carbohydrate polymers are medically and industrially important. the s-layer of many gram-positive organisms comprises protein and carbohydrate polymers and forms an almost paracrystalline array on the cell surface. not only is this array important for the bacteria but it has potential application in the manufacture of commercially important polysaccharides and glycoconjugates as well. the s-layer glycoprotein glycan from geobacillus stearothermophilus nrs 2004/3a is mainly composed of repeating ... | 2010 | 20097205 |
| a mas nmr study of the bacterial abc transporter artmp. | atp-binding cassette (abc) transport systems facilitate the translocation of substances, like amino acids, across cell membranes energised by atp hydrolysis. this work describes first structural studies on the abc transporter artmp from geobacillus stearothermophilus in native lipid environment by magic-angle spinning nmr spectroscopy. the 2d crystals of artmp and 3d crystals of isolated artp were prepared in different nucleotide-bound or -unbound states. from selectively (13)c,(15)n-labelled ar ... | 2010 | 20099290 |
| magnetic circular dichroism spectroscopic characterization of the nos-like protein from geobacillus stearothermophilus (gsnos). | nitric oxide synthase (nos) catalyzes the nadph- and o(2)-dependent oxidation of l-arginine (l-arg) to nitric oxide (no) and citrulline via an n(g)-hydroxy-l-arginine (nha) intermediate. mammalian noss have been studied quite extensively; other eukaryotes and some prokaryotes appear to express nos-like proteins comparable to the oxygenase domain of mammalian noss. in this study, a recombinant nos-like protein from the thermostable bacterium geobacillus stearothermophilus (gsnos) has been charact ... | 2010 | 20110129 |
| biosorption of heavy metals from industrial waste water by geobacillus thermodenitrificans. | the metal binding capacity of the thermophilic bacteria geobacillus thermodenitrificans isolated from damodar river, india was assessed using synthetic metal solutions and industrial waste water. biosorption preference of dead biomass of g. thermodenitrificans for the synthetic metal solutions was in the following order fe(+3)>cr(+3)>co(+2)>cu(+2)>zn(+2)>cd(+2)>ag(+)>pb(+2). it reduced the concentration of fe(+3) (91.31%), cr(+3) (80.80%), co(+2) (79.71%), cu(+2) (57.14%), zn(+2) (55.14%), cd(+2 ... | 2010 | 19864059 |
| identifying assembly-inhibiting and assembly-tolerant sites in the sbsb s-layer protein from geobacillus stearothermophilus. | surface layer (s-layer) proteins self-assemble into two-dimensional crystalline lattices that cover the cell wall of all archaea and many bacteria. we have generated assembly-negative protein variants of high solubility that will facilitate high-resolution structure determination. assembly-negative versions of the s-layer protein sbsb from geobacillus stearothermophilus pv72/p2 were obtained using an insertion mutagenesis screen. the haemagglutinin epitope tag was inserted at 23 amino acid posit ... | 2010 | 19836402 |
| residue tyr224 is critical for the thermostability of geobacillus sp. rd-2 lipase. | a thermophilic lipase (lipgrd) from geobacillus sp. rd-2, isolated from a hot spring in yunnan, china, was cloned and over-expressed in escherichia coli. the function of the conserved residue, tyr224, near the presumed temperature switch site was analyzed by site-directed saturation mutagenesis. the activity of the wild type lipgrd was optimal at 55 degrees c and ph 7.5, but that from mutant y224c was optimally active at 35 degrees c, whereas y224p lipase was optimally active at 65 degrees c. fu ... | 2010 | 19763406 |
| a new xylanase from thermoacidophilic alicyclobacillus sp. a4 with broad-range ph activity and ph stability. | we have identified a highly ph-adaptable and stable xylanase (xyna4) from the thermoacidophilic alicyclobacillus sp. a4, a strain that was isolated from a hot spring in yunnan province, china. the gene (xyna4) that encodes this xylanase was cloned, sequenced, and expressed in escherichia coli. it encodes a 338-residue polypeptide with a calculated molecular mass of 42.5 kda. the deduced amino acid sequence is most similar to (53% identity) an endo-1,4-beta-xylanase from geobacillus stearothermop ... | 2010 | 19916085 |
| ribosomal interaction of bacillus stearothermophilus translation initiation factor if2: characterization of the active sites. | infb-encoded translation initiation factor if2 contains a non-conserved n-terminal domain and two conserved domains (g and c) constituted by three (g1, g2 and g3) and two (c1 and c2) sub-domains. here, we show that: (i) bacillus stearothermophilus if2 complements in vivo an escherichia coli infb null mutation and (ii) the n-domain of b. stearothermophilus if2, like that of e. coli if2, provides a strong yet dispensable interaction with 30 s and 50 s subunits in spite of the lack of any size, seq ... | 2010 | 19917289 |
| endo- and exo-inulinases: enzyme-substrate interaction and rational immobilization. | three-dimensional models of exoinulinase from bacillus stearothermophilus and endoinulinase from aspergillus niger were built up by means of homology modeling. the crystal structure of exoinulinase from aspergillus awamori was used as a template, which is the sole structure of inulinase resolved so far. docking and molecular dynamics simulations were performed to investigate the differences between the two inulinases in terms of substrate selectivity. the analysis of the structural differences b ... | 2010 | 19941325 |
| purification and characterization of a thermostable phytate resistant alpha-amylase from geobacillus sp. lh8. | a thermophilic and amylolytic bacterium (lh8) was isolated from the hot spring of larijan in iran at 65 degrees c. identification of strain lh8 by 16s rdna sequence analysis showed that lh8 strain belongs to the geobacillus sp. with 99% sequence similarity with the 16s rdna of geobacillus thermodenitrificans. a new alpha-amylase (ga) was extracted from this strain and purified by ion-exchange chromatography. sds-page showed a single band with an apparent molecular mass of 52kda. the optimum temp ... | 2010 | 19874846 |
| characterization of thermostable fmn-dependent nadh azoreductase from the moderate thermophile geobacillus stearothermophilus. | the gene encoding an fmn-dependent nadh azoreductase, azrg, from thermophilic geobacillus stearothermophilus was cloned and functionally expressed in recombinant escherichia coli. purified recombinant azrg is a homodimer of 23 kda and bore fmn as a flavin cofactor. the optimal temperature of azrg was 85 degrees c for the degradation of methyl red (mr). azrg remained active for 1 h at 65 degrees c and for 1 month at 30 degrees c, demonstrating both superior thermostability and long-term stability ... | 2010 | 19997911 |
| evaluation of the vitek2 bcl card for identification of bacillus species and other aerobic endosporeformers. | to evaluate the performance of the vitek2 bacillus identification card (bcl) for the identification of aerobic endospore-forming bacteria, using fresh isolates and reference strains. | 2010 | 19943885 |
| absorption, steady-state fluorescence, fluorescence lifetime, and 2d self-assembly properties of engineered fluorescent s-layer fusion proteins of geobacillus stearothermophilus nrs 2004/3a. | s-layer fusion protein technology was used to design four different fluorescent fusion proteins with three different gfp mutants and the red fluorescent protein mrfp1. their absorption spectra, steady-state fluorescence, and fluorescence lifetime were investigated as a function of ph. it was found that fluorescence intensities and lifetime of the gfp mutant s-layer fusion proteins decreased about 50% between ph 6 and ph 5. the spectral properties of the red s-layer fusion protein were minimally ... | 2010 | 19954211 |
| on the importance of the small domain in the thermostability of thermoalkalophilic lipases from l1 and t1: insights from molecular dynamics simulation. | an all-atom level md simulation in explicit solvent at high temperature is a powerful technique to increase our knowledge about the structurally important regions modulating thermal stability in thermenzymes. in this respect, two large-sized thermoalkalophilic enzymes from bacillus stearothermophilus l1 (l1 lipase) and geobacillus zalihae strain t1 (t1 lipase) are well-established representatives. in this paper, comparative results from temperature-induced md simulations of both model systems at ... | 2010 | 19958281 |
| modification of progesterone and testosterone by a food-borne thermophile geobacillus kaustophilus. | the present work was carried out to study structural modification of steroids by geobacillus kaustophilus, a bacterial thermophile present in milk and the environment. incubation of progesterone and testosterone with g. kaustophilus at 65 degrees c resulted in oxygenated steroid nuclei. the oxygenation of the steroid molecule was stereo specific. seven metabolites of progesterone horizontal line 6beta/6alpha-hydroxytestosterone, 20-hydroxyprogesterone, 6beta-/6alpha-20-dihydroxyprogesterone, 5al ... | 2010 | 19961354 |
| dry thermal resistance of bacillus anthracis (sterne) spores and spores of other bacillus species: implications for biological agent destruction via waste incineration. | to obtain needed data on the dry thermal resistance of bacillus anthracis spores and other bacillus species for waste incinerator applications. | 2010 | 20015207 |
| proteomic analysis of interactions between a deep-sea thermophilic bacteriophage and its host at high temperature. | the virus-host interaction is essential to understanding the role that viruses play in ecological and geochemical processes in deep-sea vent ecosystems. virus-induced changes in cellular gene expression and host physiology have been studied extensively. however, the molecular mechanism of interaction between a bacteriophage and its host at high temperature remains poorly understood. in the present study, the virus-induced gene expression profile of geobacillus sp. e263, a thermophile isolated fr ... | 2010 | 20015994 |
| effectiveness of inoculation with isolated geobacillus strains in the thermophilic stage of vegetable waste composting. | an inoculum containing two amylolytic and three cellulolytic thermophilic bacteria, isolated from a preceding compost pile and identified as geobacillus species by 16s rrna gene sequencing, was applied to a mixture of market waste, rice straw and cow dung (5:1:0.2) so that the initial cell density was 2 x 10(8) colony forming unit (cfu) per gram dry weight at 55 degrees celsius. the inoculation increased the total cell count particularly in the thermophilic stage as determined by flow cytometry. ... | 2010 | 20036532 |
| roles of rubisco and the rubisco-like protein in 5-methylthioadenosine metabolism in the nonsulfur purple bacterium rhodospirillum rubrum. | ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco) catalyzes the assimilation of atmospheric co(2) into organic matter and is thus central to the existence of life on earth. the beginning of the 2000s was marked by the discovery of a new family of proteins, the rubisco-like proteins (rlps), which are structural homologs of rubisco. rlps are unable to catalyze co(2) fixation. the rlps from chlorobaculum tepidum, bacillus subtilis, geobacillus kaustophilus, and microcystis aeruginosa have b ... | 2010 | 20038587 |
| heterogeneous properties of individual molecules of beta-galactosidase from the thermophilic bacteria geobacillus stearothermophilus. | single enzyme molecule assays were performed on beta-galactosidase from the thermophilic bacteria geobacillus stearothermophilus using a capillary electrophoresis-based continuous flow assay and the substrate ddao-beta-d: -galactoside. the enzyme was found to be heterogeneous with respect to catalytic rate, electrophoretic mobility and activation energy of catalysis. catalytic rate was also found to vary over time for individual molecules at elevated temperature. comparison with beta-galactosida ... | 2010 | 20049517 |