Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [the unstable carotid stenosis: definition and biological processes]. | the occurrence of cerebral or retinal ischemic symptoms ipsilateral to high-grade internal carotid artery (ica) stenosis indicates a status of instability with a substantial risk for future major stroke. additionally, the detection of microembolic signals downstream of ica stenosis is predictive for future cerebral ischemia in asymptomatic and symptomatic patients. there is substantial evidence that in unstable ica stenosis plaque rupture and thrombus formation are the most frequent pathoanatomi ... | 2003 | 12736700 |
| effective macrophage redox defense against chlamydia pneumoniae depends on l-type ca2+ channel activation. | macrophage immune capability depends on their efficient redox potential expressed in the effective release of reactive oxygen species (ros) and nitric oxide. in this study the effect of the activation of a specialized ca(2+) channel on macrophage redox function during chlamydia pneumoniae infection was explored. c. pneumoniae exhibited a profound and sustained ca(2+) influx capacity, with evidence of activity attributable to their lipopolysaccharide (clps) content. also the organism showed an ad ... | 2003 | 12736823 |
| [late t-lymphocyte and monocyte activation in coronary restenosis. evidence for a persistent inflammatory/immune mechanism?]. | this study was made to determine if restenosis after percutaneous coronary angioplasty is associated with acute or chronic inflammatory/immunologic activity, and explored possible relationships with latent infection. | 2003 | 12737784 |
| production of chlamydia pneumoniae proteins in bacillus subtilis and their use in characterizing immune responses in the experimental infection model. | due to intracellular growth requirements, large-scale cultures of chlamydiae and purification of its proteins are difficult and laborious. to overcome these problems we produced chlamydial proteins in a heterologous host, bacillus subtilis, a gram-positive nonpathogenic bacterium. the genes of chlamydia pneumoniae major outer membrane protein (momp), the cysteine-rich outer membrane protein (omp2), and the heat shock protein (hsp60) were amplified by pcr, and the pcr products were cloned into ex ... | 2003 | 12738633 |
| failure to detect chlamydia pneumoniae by cell culture and polymerase chain reaction in major arteries of 93 patients with atherosclerosis. | to detect chlamydia pneumoniae in punch specimens of the aortic wall of 61 patients undergoing coronary-aortic bypass graft, and carotid atheromas of 32 patients undergoing carotid endarterectomy, cell culture (hep-2 cells) and two polymerase chain reaction assays in two different laboratories were used. all cultures and polymerase chain reaction tests for chlamydia pneumoniae were negative. further studies are required to explore the complex relationship between chlamydia pneumoniae and atheros ... | 2003 | 12739106 |
| community-acquired pneumonia in southeast asia: the microbial differences between ambulatory and hospitalized patients. | to determine microbial agents causing community-acquired pneumonia (cap) in southeast asia. | 2003 | 12740268 |
| t helper type 1 lymphocytes drive inflammation in human atherosclerotic lesions. | atherosclerotic lesions are infiltrated by macrophages and t lymphocytes, potentially reactive to pathogens. we studied in vivo activated t lymphocytes that infiltrate atherosclerotic plaques of helicobacter pylori-infected patients with or without anti-chlamydia pneumoniae antibodies. in all atherosclerotic lesions, t helper type 1 (th1) cells were predominant. c. pneumoniae-specific t cells were detected only in the plaques of anti-c. pneumoniae seropositive patients, whereas h. pylori-specifi ... | 2003 | 12740434 |
| lack of association between chlamydia pneumoniae seropositivity and aortic atherosclerotic plaques: a population-based transesophageal echocardiographic study. | the objective of this study was to examine the relationship between chlamydia pneumoniae seropositivity and aortic atherosclerotic plaques in the general population. | 2003 | 12742286 |
| monitoring intracellular replication of chlamydophila (chlamydia) pneumoniae in cell cultures and comparing clinical samples by real-time pcr. | strains of chlamydophila pneumoniae may be associated with respiratory disease or atherosclerosis. two real-time quantitative pcr assays targeting the species-specific genes cpn0278 and argr were developed to compare the in vitro growth of respiratory strains ar39 and k6 with that of atherosclerotic strain a03 and to quantify c. pneumoniae in clinical samples. a third real-time pcr assay was designed to assess contamination with mycoplasma spp. the assays targeting c. pneumoniae detected dna con ... | 2003 | 12742318 |
| synergistic effect of persistent chlamydia pneumoniae infection, autoimmunity, and inflammation on coronary risk. | given the role of chronic infections, autoimmunity, and inflammation in atherosclerosis, we studied the joint effect of chronic chlamydia pneumoniae infection, persistently elevated human heat-shock protein 60 (hhsp60) antibodies, and c-reactive protein (crp) on coronary risk. | 2003 | 12743003 |
| dendritic cells co-localize with activated cd4+ t cells in giant cell arteritis. | giant cell arteritis (gca) is a vasculitis predominantly affecting medium- and large-sized arteries. recent data show the co-localization of dendritic cells and chlamydia pneumoniae in vascular biopsies from gca patients. here we define the topographical relation of dendritic cells and these activated t-cells to determine the antigen presenting cell in gca, and to examine several auxiliary biochemical and genetic aspects relating to the role of bacteria such as c. pneumoniae in eliciting gca. | 2003 | 12747272 |
| higher prevalence of chlamydia pneumoniae seropositivity in finnish twins compared with co-twins living in sweden: relationships with markers of subclinical atherosclerosis. | mortality rates from coronary heart disease are lower in swedish men and among finnish migrants who have lived in sweden for over 20 years than in men living in finland. sero-epidemiological studies, investigations of atheromatous plaque specimens, in vitro animal models and anti-chlamydial antibiotic trials have given support to the hypothesis that chlamydia pneumoniae (cpn) has a role in atherosclerosis. we investigated whether men with a similar genetic background but living permanently in fi ... | 2003 | 12747799 |
| egr-1, a major link between infection and atherosclerosis? | 2003 | 12750307 | |
| failure to detect chlamydia pneumoniae dna in cerebral aneurysmal sac tissue with two different polymerase chain reaction methods. | chlamydia pneumoniae (c pneumoniae) is a common cause of a usually mild, community acquired pneumonia. this organism, however, can spread from the respiratory tract into other parts of the body and has been detected in up to 70% of atheromatous lesions in blood vessels. although the exact mechanism of the c pneumoniae contribution to the pathogenesis of atherosclerosis remains unknown, prophylactic antibiotic trials are planned for people at high risk for coronary disease. | 2003 | 12754346 |
| chlamydia pneumoniae dna in patients with symptomatic carotid atherosclerotic disease. | we evaluated the correlation between the presence of chlamydia pneumoniae in atherosclerotic carotid plaques, lymph nodes, and peripheral blood mononuclear cells (pbmcs), and symptomatic carotid atherosclerotic disease. | 2003 | 12756349 |
| hit-hit and hit-run: viruses in the playing field of multiple sclerosis. | viruses have been major players in the search for the cause of multiple sclerosis (ms). in support of the viral theory is the predominance of cd8+ t cells and class-i major histocompatibility complex in lesions, the powerful therapeutic effects of beta interferons, the ease of inducing demyelination in experimental models following virus challenge, and the documented examples of several human demyelinating diseases conclusively demonstrated to be of viral origin. we propose two hypotheses of how ... | 2003 | 12760396 |
| role of infections in atherogenesis. | 2003 | 12760584 | |
| chlamydia pneumoniae resists antibiotics in lymphocytes. | chlamydia pneumoniae infection of lymphocytes in blood has been well documented, and it is apparent that control of this pathogen in these cells may be critical in the development of chronic inflammatory diseases associated with infection by this bacterium. the activity of antibiotics against c. pneumoniae in lymphocytes was assessed in this study by utilizing an in vitro infection model with lymphoid cells. the results obtained indicated that although all of the antibiotics tested showed remark ... | 2003 | 12760877 |
| involvement of nicotinic acetylcholine receptors in controlling chlamydia pneumoniae growth in epithelial hep-2 cells. | nicotinic acetylcholine receptors (nachrs) play an essential role in neurotransmission. recent studies have indicated that nachrs may be involved in the regulation of some bacterial infections through immunological mechanisms in macrophages. however, the regulation of infection with chlamydia pneumoniae, which is a ubiquitous pneumonia-causing bacterium, by an nachr-mediated mechanism is still unclear. in the present study, it was found that stimulation of nachrs with ligands such as nicotine an ... | 2003 | 12761154 |
| induction of oral tolerance as treatment or prevention of chronic diseases associated with chlamydia pneumoniae infection--hypothesis. | chlamydia pneumoniae infection is associated with chronic diseases such as asthma, reactive arthritis, and atherosclerosis. several investigations and experimental results indicate an excessive immune response to heat shock protein (hsp) 60 as a possible common pathogenetic link between chlamydia pneumoniae infection and the associated chronic diseases. | 2003 | 12761463 |
| effect of short-term treatment with azithromycin on recurrent ischaemic events in patients with acute coronary syndrome in the azithromycin in acute coronary syndrome (azacs) trial: a randomised controlled trial. | there is serological and epidemiological evidence of an association between chlamydia pneumoniae infection and coronary artery disease. results of previous smaller studies have indicated a reduction of recurrent ischaemic events in patients with acute coronary syndrome when given macrolide antibiotics. we aimed to assess whether short-term treatment with the macrolide antibiotic azithromycin reduces recurrent ischaemic events in patients admitted for unstable angina or myocardial infarction. | 2003 | 12642046 |
| cloning, expression and purification of three chaperonin 60 homologues. | the chaperonin 60 (cpn60) proteins have, in addition to their well-known functions of protein folding and protection, a range of intercellular signalling activities. as part of a study to investigate the biological activity of the cpn60 proteins, particularly from pathogenic organisms, we have cloned and expressed three cpn60 proteins from homo sapiens, helicobacter pylori and chlamydia pneumoniae. the cpn60 proteins were purified to apparent homogeneity using a combination of nickel column affi ... | 2003 | 12651007 |
| chlamydia pneumoniae and atherosclerosis. | chlamydia pneumoniae is a human respiratory pathogen that causes acute respiratory disease. multiple studies have associated c. pneumoniae with cardiovascular disease including seroepidemiologic studies, direct detection of the organism within the lesion, and isolation of the organism from atheromatous tissue. the most critical question to be answered by researchers in the field is whether c. pneumoniae plays a role in atherogenesis. this review summarizes in vitro studies, results in animal mod ... | 2003 | 12652454 |
| in vitro activity of a new antibiotic, nvp-pdf386 (vrc4887), against chlamydia pneumoniae. | the in vitro activity of nvp-pdf386 (vrc4887), a novel new peptide deformylase inhibitor, and those of levofloxacin and clarithromycin were tested against 21 isolates of chlamydia pneumoniae. the mic at which 90% of the isolates were inhibited and the minimal bactericidal concentration at which 90% of the isolates were killed by nvp-pdf386 for all isolates of c. pneumoniae were 0.008 micro g/ml (range, 0.008 to 0.015 micro g/ml) compared to 0.25 and 0.06 micro g/ml for levofloxacin and clarithro ... | 2003 | 12654690 |
| microbiological efficacy of abt-773 (cethromycin) for the treatment of community-acquired pneumonia due to chlamydia pneumoniae. | nasopharyngeal specimens for culture of chlamydia pneumoniae were obtained from patients with community-acquired pneumonia enrolled in a randomized study comparing the novel ketolide antibiotic abt-773 at a dose of 150 mg once a day to 150 mg twice a day, by mouth for 10 days. c. pneumoniae was eradicated from the nasopharynx of 10 of 10 (100%) microbiologically evaluable patients. mics and mbcs for 13 isolates of c. pneumoniae from 12 patients obtained before and after therapy were performed ag ... | 2003 | 12654764 |
| [no detection of chlamydia pneumoniae in normal and atherosclerotic femoral arteries by polymerase chain reaction (pcr)--an autopsy study]. | since saikku's investigations in 1988 a direct relation between atherosclerosis and infection with chlamydia pneumoniae has been suspected. the aim of the present study was to examine histologically normal and atherosclerotic femoral arteries of autopsy cases and to correlate histological findings with the presence of chlamydia pneumoniae which was tested with nested polymerase chain reaction. the study included all of the femoral arteries (of which 16 male, 15 female, aged between 50 and 88 yea ... | 2003 | 12658470 |
| antibodies against chlamydia pneumoniae in stable angina and interleukin-6 levels. | 2003 | 12659992 | |
| meta-analysis of sero-epidemiologic studies of the relation between chlamydia pneumoniae and atherosclerosis: does study design influence results? | the results of sero-epidemiologic studies of the relation between chlamydia pneumoniae and atherogenesis vary considerably. a meta-analysis was performed to calculate the overall odds ratio (or) and to identify potential sources of variability. | 2003 | 12660662 |
| chlamydia pneumoniae infection of alveolar macrophages: a model. | chlamydia (chlamydophila) pneumoniae is a common respiratory pathogen, and it seems likely that alveolar macrophages may have an important role in infection with this bacterium. in the present study, we examined the usefulness of a continuous cell line of murine alveolar macrophages, designated "mh-s," as an in vitro c. pneumoniae infection model. infection of mh-s cells with c. pneumoniae resulted in the development of typical inclusion bodies in the cells, similar to that seen in primary alveo ... | 2003 | 12660925 |
| recruitment of chlamydia pneumoniae-infected macrophages to the carotid artery wall in noninfected, nonatherosclerotic mice. | monocyte recruitment into the subendothelium is a crucial step in atherogenesis. chlamydia pneumoniae resides in circulating monocytes and in the atherosclerotic vascular wall. however, the role of c pneumoniae for monocyte recruitment is unknown. the aim of this study was to examine the impact of c pneumoniae on monocyte adhesion and migration. | 2003 | 12663369 |
| chlamydia pneumoniae, but not bartonella quintana, is associated with coronary heart disease: results of a french case-control study. | serologic cross-reactivity has been demonstrated between bartonella quintana and chlamydia pneumoniae. therefore, the association between antibodies to c. pneumoniae and coronary heart disease (chd) as described in the literature may be due to antibodies cross-reacting with b. quintana. to investigate this hypothesis, we evaluated, in a case-control study, the prevalence of c. pneumoniae and b. quintana antibodies among 296 cases with angiographically significant artery lesions and 170 controls ... | 2003 | 12667243 |
| a cluster of cases of severe acute respiratory syndrome in hong kong. | information on the clinical features of the severe acute respiratory syndrome (sars) will be of value to physicians caring for patients suspected of having this disorder. | 2003 | 12671062 |
| chlamydia pneumoniae affect surfactant trafficking and secretion due to changes of type ii cell cytoskeleton. | understanding the surfactant dysfunction by gram-negative bacteria pulmonary infection, the intracellular fate of chlamydia pneumoniae (cpn), its interaction with uptake, recycling, and secretion of surfactant and with the cytoskeleton of type ii pneumocytes was investigated. bacteria colocalized with surfactant protein (sp)-a-mediated endocytosed lipid and early endosomes (eea1- and rab5-positive) after 3 and 6 h of infection. no specific contact with late endosomes (rab7- and m6pr-positive), l ... | 2003 | 12676805 |
| a family with abdominal aortic aneurysms. | this investigation focused on 7 siblings to 2 brothers with abdominal aortic aneurysm (aaa), with respect to aaa, chlamydia pneumoniae (cp) serology, serum cholesterol, and smoking habits. five male and 4 female siblings were included. all siblings underwent ultrasonography, and surgical specimens from the aorta were prepared for immunohistochemical (ihc) analysis. blood was obtained from all living siblings and serum cholesterol level was analyzed. serologic analysis was done by microimmunofluo ... | 2003 | 12678192 |
| in vitro antibiotic activity against chlamydia pneumoniae clinical isolates. | 2003 | 12678424 | |
| absence of association between infectious agents and endothelial function in healthy young men. | although several studies have reported correlations between infections and coronary artery disease, associations with endothelial dysfunction, its precursor, have not been established. this study assessed whether infection with chlamydia pneumoniae (cp), cytomegalovirus (cmv), epstein-barr virus (ebv), or helicobacter pylori (hp) is associated with decreased endothelial function. | 2003 | 12681997 |
| distribution of chlamydia pneumoniae dna in atherosclerotic carotid arteries: significance for sampling procedures. | despite extensive efforts to confirm a direct association between chlamydia pneumoniae and atherosclerosis, different laboratories continue to report a large variability in detection rates. in this study, we analyzed multiple sections from atherosclerotic carotid arteries from 10 endartectomy patients to determine the location of c. pneumoniae dna and the number of sections of the plaque required for analysis to obtain a 95% confidence of detecting the bacterium. a sensitive nested pcr assay det ... | 2003 | 12682129 |
| genome sequence of chlamydophila caviae (chlamydia psittaci gpic): examining the role of niche-specific genes in the evolution of the chlamydiaceae. | the genome of chlamydophila caviae (formerly chlamydia psittaci, gpic isolate) (1 173 390 nt with a plasmid of 7966 nt) was determined, representing the fourth species with a complete genome sequence from the chlamydiaceae family of obligate intracellular bacterial pathogens. of 1009 annotated genes, 798 were conserved in all three other completed chlamydiaceae genomes. the c.caviae genome contains 68 genes that lack orthologs in any other completed chlamydial genomes, including tryptophan and t ... | 2003 | 12682364 |
| chlamydia pneumoniae antibodies and endothelial function as assessed by flow-mediated dilation in patients with chest pain with and without ischemic heart disease. | 2003 | 12686343 | |
| detection of chlamydia pneumoniae in peripheral blood mononuclear cells: correlation with inflammation and atherosclerosis in haemodialysis patients. | chlamydia pneumoniae has been implicated as an inflammatory agent in atherosclerosis. clinical studies in this field have yielded conflicting results, which may have resulted from a lack of standardization for c.pneumoniae detection. we attempted to accurately estimate c.pneumoniae prevalence and to examine whether c.pneumoniae is associated with atherosclerosis and inflammation in haemodialysis (hd) patients. to do this, we assessed c.pneumoniae presence by a combination of methods and correlat ... | 2003 | 12686665 |
| development of a multiplex real-time quantitative pcr assay to detect chlamydia pneumoniae, legionella pneumophila and mycoplasma pneumoniae in respiratory tract secretions. | atypical pathogens such as chlamydia pneumoniae, legionella pneumophila and mycoplasma pneumoniae are an important cause of community-acquired pneumonia. the available detection methods (culture and serology) either lack sensitivity or give only a retrospective diagnosis. in order to improve their detection and quantification in respiratory samples, a real-time multiplex pcr, performed in two separate reactions, was developed for these three pathogens. the comparison of multiplex real-time and c ... | 2003 | 12614979 |
| chlamydia pneumoniae induces neointima formation in coronary arteries of normal pigs. | we evaluated the role of intracoronary, intrapulmonary and macrophage-mediated delivery of c. pneumoniae (cp) on coronary lesion formation. | 2003 | 12618245 |
| chlamydial lps antibodies, intima-media thickness and ischemic events in patients with established atherosclerosis. | chlamydia pneumoniae has been associated with cardiovascular disease. however, studies on the presence of chlamydial antibodies and intima-media thickness (imt) or future ischemic events are inconclusive. we examined the relation between circulating antibodies to chlamydial lipopolysaccharide (clps ab), imt and the occurrence of ischemic events during follow-up in patients with manifest atherosclerotic disease. | 2003 | 12618269 |
| limited association of chlamydia pneumoniae detection with coronary atherosclerosis. | the association of chlamydia pneumoniae (c. pneumoniae) detection with atherosclerosis has been controversial because of recent conflicting results. in order to assess how and to what extent c. pneumoniae detection contributes to atherosclerosis, the association between immunohistochemical detection of c. pneumoniae antigen, intimal lesions, and the intimal thickening ratio was examined in 1674 left anterior descending coronary arterial segments from 100 autopsied japanese patients being free fr ... | 2003 | 12618271 |
| [an atypical infectious paraplegia]. | spinal cord abscess is an unusual cause of progressive paraplegia. we report the case of a 75-year-old man who presented chlamydia pneumoniae bronchopulmonary infection with secondary spinal cord localization. little clinical improvement was observed with antibiotic therapy, but the neuroradiological course was favorable. | 2003 | 12618658 |
| etiology of acute lower respiratory tract infection. | to identify pathogens responsible for acute severe lower respiratory tract infection (alrti) in under five children by non-invasive methods. | 2003 | 12619950 |
| differential expression of genes encoding membrane proteins between acute and continuous chlamydia pneumoniae infections. | chlamydia pneumoniae is associated with several chronic human diseases, including chronic obstructive pulmonary disease and atherosclerotic cardiovascular disease. during chronic disease, organisms are believed to exist in a persistent phase that is not well understood at the genetic level. long-term in vitro continuous infections are spontaneously persistent and are less susceptible than in vitro acute infections to treatment with antibiotics, and are therefore particularly relevant as an in vi ... | 2003 | 12620380 |
| infectious agents and multiple sclerosis--are chlamydia pneumoniae and human herpes virus 6 involved? | a good deal of evidence suggests an infectious component in the development of multiple sclerosis (ms) and, to date, some 20 bacteria and viruses have been associated with the disease. recent independent sets of studies have implicated the respiratory bacterium chlamydia pneumoniae and human herpes virus 6 (hhv-6) in the pathogenesis of ms. however, as is the case for essentially all earlier microbial associations, experimental evidence linking either this bacterium or this virus to ms is equivo ... | 2003 | 12620637 |
| molecular identification and antibody testing of chlamydophila pneumoniae in a subgroup of patients with hiv-associated dementia complex. preliminary results. | chlamydophila pneumoniae dna was investigated by polymerase chain reaction (pcr), in cerebrospinal fluid (csf) specimens from patients suffering from hiv-1-associated dementia complex (hadc). four (17.3%) cases of c. pneumoniae infection were identified among 23 hadc individuals with dna amplification of major outer membrane protein (momp) gene and 16s rrna gene sequences. sequence analysis revealed significant homologies with c. pneumoniae compared to chlamydia trachomatis and chlamydia psittac ... | 2003 | 12620657 |
| chlamydia pneumoniae infection in adult patients with persistent cough. | chlamydia pneumoniae is a frequent causative agent of acute respiratory disease. to assess whether c. pneumoniae plays a role in persistent cough, the prevalence of c. pneumoniae infection in adult patients with persistent cough was investigated. nasopharyngeal swabs and serology samples from 366 adult patients with a persistent cough lasting in excess of 2 weeks and 106 control subjects were analysed for bacterial isolation and by pcr. c. pneumoniae was isolated from two patients and from none ... | 2003 | 12621093 |
| combined role of the lewis antigenic system, chlamydia pneumoniae, and c-reactive protein in unstable angina. | the goal of this study was to assess the prognostic role of the lewis antigenic system, chlamydia pneumoniae (cp) seropositivity (cp+), and c-reactive protein (crp) levels in unstable angina (ua). | 2003 | 12598063 |
| chlamydia pneumoniae induces tissue factor expression in mouse macrophages via activation of egr-1 and the mek-erk1/2 pathway. | recent studies have suggested that infection with chlamydia pneumoniae (c pneumoniae) may contribute to the instability of atherosclerotic plaques and thrombosis and is associated with acute coronary events. tissue factor (tf), a potent prothrombotic molecule, is expressed by macrophages and other cell types within atherosclerotic lesions and plays an essential role in thrombus formation after plaque rupture. therefore the effects of c pneumoniae on induction of tf expression in macrophages were ... | 2003 | 12600889 |
| impact of chlamydia pneumoniae infection on survival rate after heart transplantation. | as chlamydia pneumoniae (cp), a common cause of respiratory infection, is of vasotropic character, chronic infection may be associated with the development of coronary disease, although there have been few reports on the impact of cp infection on the post-orthotopic heart transplantation (oht) survival rate. | 2003 | 12601289 |
| c-reactive protein, helicobacter pylori, chlamydia pneumoniae, cytomegalovirus and risk for myocardial infarction. | c-reactive protein (crp), chlamydia pneumonia, helicobacter pylori, and cytomegalovirus (cmv) have each been associated with atherosclerosis. we assessed how infection and crp related to risk for subsequent myocardial infarction (mi). | 2003 | 12604160 |
| in vitro activities of rifamycin derivatives abi-1648 (rifalazil, krm-1648), abi-1657, and abi-1131 against chlamydia trachomatis and recent clinical isolates of chlamydia pneumoniae. | abi-1648 (rifalazil) is a semisynthetic rifamycin with potent bactericidal activity against intracellular respiratory bacteria, including mycobacterium tuberculosis, and a long half-life (approximately 60 h) and thus can be administered once weekly. we therefore tested the in vitro activities of abi-1648, its derivatives abi-1657 and abi-1131, azithromycin, and levofloxacin against 10 strains of chlamydia trachomatis and 10 recent clinical isolates of chlamydia pneumoniae. the mics at which 90% ... | 2003 | 12604555 |
| chlamydia pneumoniae and multiple sclerosis: the latest etiologic candidate. | 2003 | 12606874 | |
| infection with chlamydia pneumoniae and risk of multiple sclerosis. | chlamydia pneumoniae (cpn) has been proposed as a possible etiologic agent for multiple sclerosis (ms), but results of previous studies are conflicting. | 2003 | 12606878 |
| measurement of igg antibodies to chlamydia trachomatis by commercial enzyme immunoassays and immunofluorescence in sera from pregnant women and patients with infertility, pelvic inflammatory disease, ectopic pregnancy, and laboratory diagnosed chlamydia psittaci/chlamydia pneumoniae infection. | screening for chlamydia trachomatis specific antibodies is valuable in diagnosing asymptomatic pelvic inflammatory disease (pid) and tubal damage following repeated episodes of pid. the assays in current use are unsuitable for screening large numbers of samples so there is a need to develop more suitable assays. | 2003 | 12610104 |
| infections preceding early arthritis in southern sweden: a prospective population-based study. | to detect evidence of infections preceding early arthritis in southern sweden and to compare the clinical outcome of remission during a 6-month followup for patients with and without signs of prior infection. | 2003 | 12610801 |
| multiple infections and subsequent cardiovascular events in the heart outcomes prevention evaluation (hope) study. | limited prospective epidemiological data are available on the relation between exposure to chlamydia pneumoniae, helicobacter pylori, cytomegalovirus (cmv), and hepatitis a virus (hav), individually or as a total pathogen score, and human cardiovascular (cv) disease. | 2003 | 12538424 |
| induction of proinflammatory cytokines in human lung epithelial cells during chlamydia pneumoniae infection. | chlamydia pneumoniae is an obligate intracellular human pathogen that causes acute respiratory diseases such as pneumonia and bronchitis. previous studies have established that c. pneumoniae can induce cytokines in mouse and/or human cells, but little information is available on the cytokine response of respiratory epithelial cells, a first line of infection. in this study, heparin treatment of c. pneumoniae significantly reduced its ability to induce interleukin 8 (il-8) and tumor necrosis fact ... | 2003 | 12540537 |
| methodologies and cell lines used for antimicrobial susceptibility testing of chlamydia spp. | in vitro susceptibility testing was performed on strains of chlamydia trachomatis, chlamydia pneumoniae, and chlamydia psittaci under various conditions, including the cell line utilized, the time between infection and the addition of an antimicrobial, the concentration of inoculum, and the effect of multiple passage on the minimal chlamydicidal concentrations for the antibiotics doxycycline, azithromycin, erythromycin, ofloxacin, and tetracycline. with macrolides, the mic varied depending upon ... | 2003 | 12543671 |
| chlamydia pneumoniae and acute chest syndrome in patients with sickle cell disease. | purpose few studies address the association of chlamydia pneumoniae infection with pulmonary disease and outcome in patients with underlying pathology such as sickle cell disease (scd). scd patients are susceptible to the pulmonary disorder known as acute chest syndrome (acs), where the etiology remains ill defined. the purpose of this study was to analyze the clinical course and outcome of c. pneumoniae-associated acs among scd patients as part of the national acute chest syndrome study. | 2003 | 12544773 |
| qsyp peptide sequence is selected from phage display libraries by bovine igg contaminants in monoclonal antibody preparations. | a consensus peptide sequence, qsyp, appears as an artifact during the mapping of monoclonal antibodies (mabs) using a random peptide phage display library. phage bearing this qsyp sequence were independently selected by four different laboratories screening separate mab preparations with the same phage library. in each case, the qsyp sequence was selected in addition to a consensus sequence specific to the mab. phage that displayed the qsyp sequence were not bound by the mab of interest, but rat ... | 2003 | 12545550 |
| cell-to-cell contact of human monocytes with infected arterial smooth-muscle cells enhances growth of chlamydia pneumoniae. | chlamydia pneumoniae can infect arterial cells. it has been shown that coculture of human monocytes (u937) and endothelial cells promotes infection of c. pneumoniae in endothelial cells and that the enhancement was mediated by a soluble factor (insulin-like growth factor 2) secreted by monocytes. in this study, it is shown that coculture of monocytes with c. pneumoniae enhances infection of c. pneumoniae in arterial smooth-muscle cells 5.3-fold at a monocyte-to-smooth-muscle cell ratio of 5. how ... | 2003 | 12552427 |
| role of viruses and atypical bacteria in exacerbations of asthma in hospitalized children: a prospective study in the nord-pas de calais region (france). | we studied the role of viruses and atypical bacteria in children hospitalized with exacerbated asthma by a prospective study of children with acute asthma admitted to the department of pediatrics in lille, and to 15 hospitals in the nord-pas de calais region, from october 1, 1998-june 30, 1999. we included children aged 2-16 years with active asthma, defined as three or more recurrent episodes of reversible wheezing. the severity of asthma and of asthmatic exacerbations was recorded. immunofluor ... | 2003 | 12526066 |
| different heat shock protein 60 species share pro-inflammatory activity but not binding sites on macrophages. | in a study of seven different hsp60 species, we found that all mammalian and microbial proteins shared the property of eliciting an inflammatory response in mouse macrophages. in all cases, tnfalpha production was induced by 0.1 microm concentrations of hsp60. however, the different hsp60 preparations did not compete for the same binding site. the binding of fluorescence-labeled human hsp60 was inhibited by excess unlabeled human, rat or mouse hsp60, but not hamster, escherichia coli, chlamydia ... | 2003 | 12505167 |
| measurement of chlamydia pneumoniae-specific immunoglobulin a (iga) antibodies by the microimmunofluorescence (mif) method: comparison of seven fluorescein-labeled anti-human iga conjugates in an in-house mif test using one commercial mif and one enzyme immunoassay kit. | for the serological diagnosis of acute chlamydia pneumoniae infection, the microimmunofluorescence (mif) test is the most commonly used method and also the "gold standard" for the measurement of immunoglobulin g (igg) and igm antibodies. the role of iga antibodies in diagnosis has not been established. commercially available fluorescein-labeled anti-human iga conjugates have not been systematically compared to each other, and this situation may cause considerable variations in iga results. there ... | 2003 | 12522032 |
| characterization of the humoral immune response to chlamydia outer membrane protein 2 in chlamydial infection. | detection of antibodies to an outer membrane protein 2 (omp2) by enzyme-linked immunosorbent assay (elisa) by using either the chlamydia trachomatis- or the chlamydia pneumoniae-specific protein was investigated. omp2 is an immunodominant antigen giving rise to antibody responses in humans infected with different c. trachomatis serovars (a to c and d to k) or with c. pneumoniae, which could be detected by omp2 elisa. omp2 elisa is not species specific, but antibody titers were usually higher on ... | 2003 | 12522047 |
| development potential of rifalazil. | rifalazil represents a new generation of ansamycins that contain a unique four-ring structure. originally rifalazil was developed as a therapeutic agent to replace rifampin as part of a multiple drug regimen in the treatment of tuberculosis. as a result of its superior antimicrobial activity and high intracellular levels, rifalazil has potential to treat indications caused by the intracellular pathogen, chlamydia trachomatis, which causes non-gonococcal urethritis and cervicitis, often leading t ... | 2003 | 12556219 |
| a review of macrolide treatment of atherosclerosis and abdominal aortic aneurysms. | seroepidemiological studies have shown an association between chlamydia pneumoniae and atherosclerosis, the risk of acute myocardial infarction and abdominal aortic aneurysms (aaa). several studies have detected c. pneumoniae in atherosclerotic lesions from coronary and carotid arteries, in aaa, and in sclerotic aortic valves. however, culturing of c. pneumoniae is difficult and has seldomly succeeded from atherosclerotic lesions. thus, the pathogenicity is unknown, and the significance of detec ... | 2003 | 12570733 |
| increased prevalence of chlamydophila pneumoniae but not epstein-barr antibodies in essential hypertensives. | conflicting data exist regarding the relationship between chlamydophila pneunoniae (c. pneumoniae) and hypertension. in this study, both c. pneumoniae igg and iga titres and epstein-barr virus antibody levels were measured in 146 sustained hypertensives defined by 24 h ambulatory blood pressure monitoring (abpm) and 54 normotensives. c. pneumoniae antibodies were measured by microimmunofluorescence test. igg > or = 80 and iga > or = 40 were defined as elevated antibody titres. epstein-barr antib ... | 2003 | 12571613 |
| dead bugs don't mutate: susceptibility issues in the emergence of bacterial resistance. | the global emergence of antibacterial resistance among common and atypical respiratory pathogens in the last decade necessitates the strategic application of antibacterial agents. the use of bactericidal rather than bacteriostatic agents as first-line therapy is recommended because the eradication of microorganisms serves to curtail, although not avoid, the development of bacterial resistance. bactericidal activity is achieved with specific classes of antimicrobial agents as well as by combinati ... | 2003 | 12533275 |
| hyperhomocysteinemia is linked with c. pneumoniae igg seropositivity in coronary artery disease and peripheral arterial occlusive disease. | 2003 | 12535757 | |
| lipoprotein(a), chlamydia pneumoniae, leptin and tissue plasminogen activator as risk markers for valvular aortic stenosis. | the aim of the present study was to identify risk markers for the development of valvular aortic stenosis (as). lipoprotein(a) (lp(a)) and chlamydia pneumoniae igg antibody titres in plasma and in circulating immune complexes as well as leptin and tissue plasminogen activator (t-pa) in plasma were studied. | 2003 | 12573277 |
| comparison of a new quantitative ompa-based real-time pcr taqman assay for detection of chlamydia pneumoniae dna in respiratory specimens with four conventional pcr assays. | chlamydia pneumoniae, an important respiratory pathogen, is difficult to culture, and detection rates by conventional pcrs vary considerably. a new quantitative ompa-based real-time pcr assay based on taqman technology for detection of c. pneumoniae in respiratory samples is described, and its performance in terms of sensitivity and reproducibility is compared with those of four published conventional pcrs (one single-step pcr targeting a cloned psti fragment; two nested pcrs, one targeting the ... | 2003 | 12574252 |
| seroprevalence of antibodies to chlamydia pneumoniae in women with preeclampsia. | preeclampsia shares many risk factors and pathophysiologic features with coronary heart disease. we studied whether, like atherosclerosis, preeclampsia is related to seroprevalence of immunoglobulin (ig) g antibodies to chlamydia pneumoniae. | 2003 | 12576242 |
| persistence of chlamydia pneumoniae in degenerative aortic valve stenosis indicated by heat shock protein 60 homologues. | based on the concept of chronic persistent infections with chlamydia pneumoniae among variable stressors for aortic valve degeneration, the study aim was to assess the presence of chlamydial heat shock protein (chsp) 60 and its human homologue (hhsp60) in diseased valvular tissue. | 2003 | 12578339 |
| coinfection of laboratory rats with mycoplasma pulmonis and chlamydia pneumoniae. | routine examinations of conventional outbred wistar rats in our laboratory showed increased serum levels of alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, and urea. electron microscopy and specific reactions showed c. pneumoniae and m. pulmonis in lung, liver, spleen, heart, and kidney sections. we could not exclude the fact that other infectious microorganisms detected through routine health surveillance affected the wistar rat colony; however, we have not identifie ... | 2003 | 12580575 |
| rapid and standardized detection of chlamydia pneumoniae using lightcycler real-time fluorescence pcr. | in order to meet the need of many microbiological laboratories for a standardized system for detecting chlamydia pneumoniae in respiratory specimens, a hybridization probe-based lightcycler (roche diagnostics, germany) pcr assay was developed. the assay's analytical sensitivity and specificity were evaluated according to the recommendations of the centers for disease control and prevention (usa) and laboratory centre for disease control (canada). seventy-four bacterial species other than chlamyd ... | 2003 | 12582746 |
| chlamydia pneumoniae infection promotes the transmigration of monocytes through human brain endothelial cells. | we have investigated the effects of chlamydia pneumoniae on human brain endothelial cells (hbmecs) and human monocytes as a mechanism for breaching the blood-brain barrier (bbb) in alzheimer's disease (ad). hbmecs and peripheral blood monocytes may be key components in controlling the entry of c. pneumoniae into the human brain. our results indicate that c. pneumoniae infects blood vessels and monocytes in ad brain tissues compared with normal brain tissue. c. pneumoniae infection stimulates tra ... | 2003 | 12584732 |
| extraction of chlamydia pneumoniae dna from vascular tissue for use in pcr: an evaluation of four procedures. | the objective of this study was to compare four procedures for chlamydia pneumoniae dna extraction from vascular tissue. the nuclisens kit, the qiaamp tissue dna minikit, buffer-saturated phenol and the geneclean ii kit were evaluated, based on the yield of recovered dna, using pcr to detect c. pneumoniae in vascular tissue. the qiaamp tissue procedure had the highest detection level (0.004 inclusion-forming units/sample). all methods, except nuclisens (70 min), had a short handling time (30-40 ... | 2003 | 12588334 |
| chlamydia pneumoniae in elderly patients with stroke (c-peps): a case-control study on the seroprevalence of chlamydia pneumoniae in elderly patients with acute cerebrovascular disease. | multiple studies have suggested an association between chlamydia pneumoniae infection and atherosclerotic vascular disease. we investigated whether serological markers of c. pneumoniae infection were associated with acute stroke or transient ischaemic attack (tia), exclusively in elderly patients. | 2003 | 12499705 |
| role of igg-seropositivity to chlamydia pneumoniae in early thrombotic events after coronary stent placement. | since infection of endothelial or smooth muscle cells with chlamydia pneumoniae increased expression of tissue factor and plasminogen activator inhibitor i (pai-1), c. pneumoniae might be involved in triggering acute thrombotic events in patients with coronary artery disease. therefore, we explored a potential relationship between igg-seropositivity to c. pneumoniae and early thrombotic events after coronary stent placement. | 2003 | 12482564 |
| serum procalcitonin concentrations in bacterial pneumonia in children: a negative result in primary healthcare settings. | a microbe-specific diagnosis in community-acquired pneumonia (cap) is difficult in children, and studies on nonspecific chest radiographic and host response markers have been inconsistent. serum procalcitonin (pct) is a newly recognized, promising marker for differentiating between bacterial and viral infections. serum pct was measured by a luminometric assay in 190 children with cap diagnosed in the primary healthcare setting during a population-based study in a geographically defined populatio ... | 2003 | 12461740 |
| chlamydial infections of the cardiovascular system. | this paper presents a review on cardiovascular diseases which can be caused by chlamydial infection with the emphasis in the recent development in association between chlamydia pneumoniae and cardiovascular disease. the review includes seroepidemiologic observations; the discovery of c. pneumoniae in atheromatous plaques; in vivo studies using animal models indicating that c. pneumoniae is a co-risk factor of hyperlipidemia for atherosclerosis; in vitro studies demonstrating putative mechanisms ... | 2003 | 12456307 |
| atypical pathogen infection in adults with acute exacerbation of bronchial asthma. | in a serologically based prospective study, acute infections with four atypical pathogens were determined in 100 adults hospitalized for acute exacerbation of bronchial asthma, and compared with the corresponding rate in a matched control group. paired sera were tested using immunofluorescence or enzyme immunoassay methods to establish the serologic diagnosis. in 18 patients (18%), there was evidence of acute infection with mycoplasma pneumoniae, compared with 3% in the control group (p = 0.0006 ... | 2003 | 12426232 |
| [studies on the infection status of seven species mycoplasma, three species of chlamydia, neisseria gonorrhoeae and garderella vaginalis in 76 patients with sexual transmitted diseases]. | to study the infectious status of seven species of mycoplasma, three species of chlamydia, neisseria gonorrhoeae and garderella vaginalis in the 76 male sexual transmitted disease (std) patients in yangzhou city. | 2004 | 15132870 |
| inflammation, infection, and aortic valve sclerosis; insights from the olmsted county (minnesota) population. | atherosclerosis-related mechanisms, including inflammation and possibly infection, are likely to be involved in the pathogenesis of calcific aortic valve disease. the purpose of this study was to examine whether systemic inflammatory markers and chlamydia pneumoniae seropositivity are associated with aortic valve sclerosis (avs) in a sample of the general population. transesophageal echocardiography was performed in 381 subjects (median age: 67 years, range: 51-101; 52% men), a sample of the adu ... | 2004 | 15136064 |
| interlaboratory reliability of microimmunofluorescence test for measurement of chlamydia pneumoniae-specific immunoglobulin a and g antibody titers. | to evaluate the reliability of chlamydia pneumoniae-specific immunoglobulin g (igg) and iga antibody titers as measured by the microimmunofluorescence (mif) test, we compared results from 392 individuals using a standard mif protocol at two academic laboratories. the kappas for dichotomous titers (>/=16 versus <16) were 0.39 for iga and 0.53 for igg. measurement error likely attenuates c. pneumoniae-disease associations; the magnitude of attenuation can be estimated from results of studies such ... | 2004 | 15138190 |
| chlamydia pneumoniae infection in young stroke patients: a case--control study. | retrospective and cross-sectional studies have suggested that both bacterial and viral infections may be risk factors for atherosclerosis, ischemic stroke and acute coronary events. the correlation between chlamydia pneumoniae and atherosclerosis remains a source of controversy. our case-control study is aimed at evaluating the frequency of c. pneumoniae infection in a cohort of young adults with recent cerebrovascular disease and in particular etiologic stroke subtypes. chlamydia pneumoniae igg ... | 2004 | 15142225 |
| is there a role for chlamydia pneumoniae infection in systemic lupus erythematosus and in the associated atherosclerotic cardiovascular disease? | to search for molecular evidence of chlamydial infection in systemic lupus erythematosus (sle) subjects and to assess if there is an association of this infectious agent with coronary artery calcification (cac), a marker of total atherosclerotic burden. | 2004 | 15144130 |
| effects of glucocorticoids on the respiratory burst of chlamydia-primed thp-1 cells. | we previously observed that the respiratory burst of human monocytes (thp-1 cell line) triggered by phorbol myristate acetate was strongly enhanced by a priming of the cells by chlamydia pneumoniae [biochem. biophys. res. commun. 287 (2001) 781]. we describe here the modifications of the responses of chlamydia-primed thp-1 cells to hydrocortisone (hct) and methylprednisolone (mpl). hct and mpl inhibited the production of the cytokines tnfalpha and il-8. but hct, which inhibited the respiratory b ... | 2004 | 15147963 |
| is chlamydia associated with alzheimer's? | 2004 | 15149617 | |
| [chlamydia pneumoniae as a pathogenetic risk factor in the development of arteriosclerosis and its complications]. | the literature data and the results of the authors' experiments allow one to consider chlamydia pneumoniae as one of the leading risk factor of development of atherosclerosis and ischemic heart disease. features of atherosclerosis morphogenesis in the presence of chlamydia are described. data, showing similarity of cell reactions, characterizing immune inflammation in mldl deposit in arterial wall and obligate parasites are described. synergism in the action of mldl and chlamydial infection may ... | 2004 | 15154386 |
| prevalence of chlamydia pneumoniae infection in squamous cell carcinoma of the head and neck. | on the basis of epidemiological data, an association between chlamydia pneumoniae (cp) infection and head and neck cancer might be suggested. the aim of the present study was to detect cp-dna within tumour tissue specimens by a two-step polymerase chain reaction. investigation was planned on the fleming's procedure for early termination when initial results were extreme. so, after ten consecutive patients, only one tumour contained cp-dna. hence the prevalence could be regarded as inferior to 60 ... | 2004 | 15154648 |
| gemifloxacin: a new fluoroquinolone. | gemifloxacin is a dual targeted fluoroquinolone with potent in vitro activity against gram-positive, -negative and atypical human pathogens--pathogens considered to be important causes of community-acquired respiratory tract infections. gemifloxacin demonstrates impressive minimal inhibitory concentrations (mic 90 ) values against clinical isolates of streptococcus pneumoniae, haemophilus influenzae, moraxella catarrhalis, chlamydia pneumoniae and legionella spp., with mic 90 values reported to ... | 2004 | 15155113 |
| in vitro activities of garenoxacin and levofloxacin against chlamydia pneumoniae are not affected by presence of mycoplasma dna. | we studied 20 chlamydia pneumoniae isolates obtained from respiratory sites and atheroma tissue of patients from various geographic areas to determine the susceptibilities of these isolates to a new des-fluoroquinolone, garenoxacin, and to levofloxacin. in addition, we assessed the cultures with these isolates by pcr for the presence or absence of mycoplasma sp. dna. both the mic at which 90% of isolates are inhibited (mic(90)) and the minimal bactericidal concentration at which 90% of isolates ... | 2004 | 15155203 |
| intracellular multiplication of legionella species and the influence of amoebae on their intracellular growth in human monocytes: mono mac 6 cells and acanthamoeba castellanii as suitable in vitro models. | legionellae are important etiological agents of pneumonia. legionella pneumophila (predominantly serogroup 1) is detected in most cases of legionellosis; other species only occasionally cause infections, predominantly in immunocompromized patients. aquiferous technical systems are the primary source of infection (air-conditioning systems, refrigerators, showers, whirlpools, springs, taps, moisturizing equipment, medical nebulizers, and swimming pools). legionellae are present in the water in the ... | 2004 | 15156026 |
| mycoplasma pneumoniae and asthma in children. | the aim of this prospective study of a population of children (age, 2-15 years) hospitalized for severe asthma was to test them for acute infection due to mycoplasma pneumoniae and acute infection due to chlamydia pneumoniae. of 119 patients with previously diagnosed asthma, acute m. pneumoniae infection was found in 24 (20%) and c. pneumoniae infection was found in 4 (3.4%) of the patients during the current exacerbation. of 51 patients experiencing their first asthma attack, acute m. pneumonia ... | 2004 | 15156467 |