Publications

the isolation of the antibody moieties of immune complexes from serum by the pepsin digestion of conglutinin-anti-conglutinin complexes.	a technique is described which allows the antibodies of circulating immune complexes to be isolated as their f(ab')2 fragments. the method is based on the precipitation of the complexes by the sequential addition of conglutinin and anti-conglutinin, and the subsequent digestion of these precipitates by pepsin. using this technique it has been possible to show antibodies to epstein-barr (eb) virus antigens in the immune complexes of patients with burkitt's lymphoma and to microbial antigens in tw ...	1981	6279342
a t-cell line derived from normal human cord leukocytes by co-culturing with human leukemic t-cells.	a unique t-cell line, mt-2, was established from normal human cord leukocytes of a male infant by co-culturing with leukemic t-cells from a female patient with adult t-cell leukemia. mt-2 cells expressed receptors for sheep erythrocytes and complement and were reactive with anti-t-cell and anti-ia sera. they were negative for fc receptors, surface immunoglobulin, and epstein-barr virus nuclear antigen. chromosomally, the mt-2 line was male and most cells were shown to have a normal diploid karyo ...	1981	6281119
acyclovir - a review of the preclinical and early clinical data of a new antiherpes drug.	a review of the literature on acyclovir (zovirax), a new antiherpes drug, with particular activity against herpes simplex virus types i and ii and also against varicella-zoster, epstein-barr, cytomegalo and herpes b viruses, is presented. the article deals with 'in vitro' and 'in vivo' efficacy in animals, animal toxicity, latency and resistance, the mechanism of action and early clinical experience.	1981	6280605
[hepatitis in infectious mononucleosis].		1981	6280643
[hepatitis caused by known viruses (other than a and b viruses)].		1981	6279919
[serological investigation of epstein-barr virus antibodies and heterophile antibodies of childhood in kumamoto prefecture (author's transl)].		1981	6279939
[the nasopharyngeal carcinoma and epstein-barr virus (ebv) specific iga (author's transl)].		1981	6280123
the epstein-barr virus and human lymphoproliferative disorders.		1981	6280236
antibody pattern to human cytomegalovirus in patients with adenocarcinoma of the colon.	the role of human cytomegalovirus (cmv) was examined according to serological patterns in 37 patients with adenocarcinoma of the colon (acc). the sera were examined for the presence of igg antibodies by the immunoperoxidase antibody to membrane antigens (ipama) method and by the complement-fixation (cf) test. antibody determinations were also performed by the ipama method for three other members of the herpesvirus group: epstein-barr virus (ebv), herpes simplex virus (hsv) and varicella-zoster ...	1981	6282775
hla antigens in adults negative for antibody to epstein-barr virus (ebv).	evidence for a genetic component in susceptibility to epstein-barr virus (ebv) infection was sought by comparing hla a and b phenotypes of ebv antibody-negative scottish medical students and randomly chosen antibody-positive controls. no statistically significant differences were observed, but three antigens, (a10, a29 and b15), were relatively underrepresented in the ebv seronegative group; findings which agree with data previously reported from a similar study in los angeles. a strong associat ...	1981	6283690
histological types of nasopharyngeal carcinoma as compared to ebv serology.	one hundred and thirteen cases of nasopharyngeal carcinoma (npc) were classified histologically according to the who-, french and cologne systems. the various histological tumor types were then correlated with data on epstein-barr virus (ebv) serology (ea, vca, ebna, cf, iga-vca). the results showed non-keratinizing carcinomas with lymphoid infiltration, and undifferentiated carcinomas to be associated with significantly elevated anti-ebv titers. these two tumor types can be easily grouped toget ...	1981	6285799
hodgkin's disease and infectious mononucleosis: is there a causal association?		1981	6286056
[viral infections of the hematopoietic system: epstein-barr-virus and other less characterized viral infections].		1981	6289545
[lymph node lesions caused by virus infections].		1981	6289546
[lymphoepithelial carcinoma of the nasopharynx: a special tumor?].		1981	6289547
[the use of nucleic acid hybridization exemplified by epstein-barr virus-associated diseases].		1981	6289561
[on the viral etiology of bell's palsy (an enzyme-linked immunosorbent assay study) (author's transl)].	in a prospective study paired sera of 14 patients suffering from bell's palsy were examined for antibodies against varicella-zoster virus (vzv), herpes-simplex virus (hsv), cytomegalovirus (cmv) and epstein-barr virus (ebv). for determination of antibodies against vzv, hsv and cmv an enzyme-linked immunosorbent assay was carried out. indirect immunofluorescence was carried out to determine antibodies against ebv. serum samples were taken within 5 days of bell's palsy having been diagnosed and we ...	1981	6285096
epstein-barr virus, clinical symptomatology and immunodeficiency in non-hodgkin's lymphoma patients.		1981	6285121
enhancement of outgrowth of eb virus-transformed cells from normal human peripheral blood by a tumor promoter, tpa.	effect of 12-0-tetradecanoyl-phorbol-13-acetate (tpa) on the establishment of lymphoblastoid cell lines (lcl) following spontaneous tranformation of b cells by epstein-barr virus (ebv) from peripheral lymphocytes of healthy ebv-seropositive adults was examined. in the lymphocyte culture with tpa (0.5 ng/ml), the frequency of establishment of lcl increased and the time required for appearance of transformation decreased. a larger number of ebv-positive cells in the peripheral blood were detected ...	1981	6270011
ebv infection of mitogen-stimulated human b lymphocytes.	b lymphocytes from human blood were treated with the mitogen protein a (staph, aureus), which induces dna synthesis in, as well as differeniation of, the b lymphocytes. the cells were subsequently exposed to epstein-barr virus (ebv). ebv-infected and mitogen-stimulated cells were detected simultaneously, by using a combination of immunofluorescence (for ebna) and autoradiography (for dna-synthesis). in the initial phase (1-2 days) after addition of the mitogen, stimulated cells were as susceptib ...	1981	6270012
difference in viral binding between two epstein-barr virus substrains to a spectrum of receptor-positive target cells.	radio-labelled epstein-barr virus (ebv) was utilized in a direct binding assay to detect the presence of ebv receptors. the sensitivity of this method was affirmed by the detection of ebv-receptors on three ev-carrying cell lines that have previously been reported as receptor negative. two laboratory substrains of ebv, derived from the cell lines b95-8 and p3hr-i (designated b and p virus respectively), were tested in the binding assay. the main repcptor prototype adsorbed both viral strains wit ...	1981	6270014
mechanisms of enhancement of epstein-barr virus-induced transformation of peripheral blood lymphocytes by a tumor promoter, tpa, with special reference to lowering of cytotoxicity of t cells.	the mechanism of the previously observed enhancement of epstein-barr virus (ebv)-induced cell transformation of human lymphocytes by 12-0-tetradecanoyl-phorbol-13-acetate (tpa) was studied. a concentration of tpa (0.5 ng/ml) was used. in cultures of human cord blood lymphocytes infected with ebv in the presence of tpa, a larger number of ebv-associated nuclear antigen (ebna)-positive and/or dna synthesizing cells was observed than in the absence of tpa. in the virus-infected lymphocyte cultures ...	1981	6270019
the effect of infection on t lymphocyte subpopulations: a preliminary report.	the effect of cytomegalovirus (cmv) infection on t-lymphocyte subpopulations (employing the monoclonal antibody okt4 to delineate helper cells and okt8 for suppressor-cytotoxic cells) was studied in 10 normal individuals with cmv mononucleosis and four renal transplant patients with symptomatic primary cmv disease. in both clinical settings, acute cmv infection is associated with a reversal of the normal helper/suppressor-cytotoxic t cell ratio, with both a relative and absolute decrease in t he ...	1981	6270027
immunofluorescent staining of nuclear antigen in lymphoid cells transformed by herpesvirus papio (hvp).	an improved fixation method for antigen detection in lymphoblastoid cells is described. herpesvirus papio nuclear antigen (hupna) could be stained in several transformed lymphoid cell lines by anti-complement immunofluorescence (acif). antibody to hupna was detected in many human sera containing antibodies to epstein-barr virus capsid and nuclear antigen (ebna). rheumatoid arthritis sera showed a high incidence of both anti-ebna and anti-hupna antibodies.	1981	6270210
the detection of epstein-barr virus receptors utilizing radiolabelled virus.	epstein-barr virus (ebv) was labelled with 3h-thymidine and purified about 1000-fold from the culture medium by ultracentrifugation on 5 to 30% dextran gradients. the presence of the virus was monitored by radioactivity and epstein-barr virus-determined nuclear antigen (ebna) induction in sensitive indicator cells (ramos). peaks for both activities occurred in the 17 to 18% dextran fractions. unlabelled virus recovered in the peak fraction was labelled with 125i. both thymidine and 125i-labelled ...	1981	6270245
the spontaneous and induced synthesis of epstein-barr virus antigens in raji cells immobilized on surface coated with anti-lymphocyte globulin.	immobilization of raji cells on surface coated with anti-lymphocyte globulin (alg) at low cell densities lead to the synthesis of epstein-barr virus (ebv) early antigen (ea) in up to 5% of the cells. at higher cell densities the percentage of antigen-positive cells decreased and at confluency no antigen synthesis was observed. addition of iododeoxyuridine (idurd) to low density cultures increased the expression of ea to 20%, whereas in confluent cultures the cells could not be induced to synthes ...	1981	6270256
epstein-barr virus-induced proteins. iv. characterization of an ebv-associated phosphopolypeptide.	cells of the epstein--barr virus (ebv) non-producing lines nc37 and raji were induced by the tumour promoter tpa and were labelled with 32p. the analysis by immunoprecipitation with human vca+ea+ sera revealed that a major phosphopolypeptide with a mol. wt. of 58 000 was specifically precipitated. in addition, a phosphopolypeptide of the same size was the dominant phosphopolypeptide detected in ebv-producing b95-8 and p3hr-1 cells as well as in p3hr-1-superinfected nc37 cells. this phosphopolype ...	1981	6270267
coexistence of rheumatoid arthritis in married couples: a search for etiological factors.	epstein-barr virus (ebv) and the genetic marker hla-dr4 both appear to play a role in the etiology of rheumatoid arthritis (ra). antibodies to viral capsid antigen and the early antigen complex of the eb virus as well as the hla phenotype were studied in 6 families. both spouses had classical or definite ra but none of their offspring, all adult, had any evidence of ra. at least 1 spouse of each couple had dr4 and this phenotype was found in 4 of the 8 offspring. all parents and their offspring ...	1981	6270332
sequence complexity of circular epstein-bar virus dna in transformed cells.	a simplified procedure, based on several methods previously used to isolate circular dna molecules from bacteria, was derived for the preparation of covalently closed circular viral dna molecules from large quantities of lymphocytes transformed by epstein-barr virus. the protocol can be applied both to virus nonproducer lines and to lines containing cells activated to virus production. sufficient amounts o highly purified viral dna of intracellular origin were obtained from b95-8 and raji cells ...	1981	6270367
defective ebv-specific suppressor t-cell function in rheumatoid arthritis.	several lines of evidence, including high antibody titers to epstein-barr virus (ebv)-associated antigens and rapid transformation of b cells into lymphoblastoid cells lines, suggest an association between ebv and rheumatoid arthritis. when lymphocytes from normal immune donors were infected with ebv in culture, they produced an exponentially increasing number of immunoglobulin-secreting cells for eight to 10 days. thereafter, there was a marked late suppression of their response, mediated by im ...	1981	6270556
deficiency of t helper cells in transient hypogammaglobulinemia of infancy.	we studied 17 patients with transient hypogammaglobulinemia of infancy to define the immunologic defect responsible for this disorder. the number of circulating b cells in these patients was normal, as was the ability of the b cells to synthesize immunoglobulins when stimulated with epstein-barr virus, a direct b-cell activator. however, the capacity of the b cells to synthesize igg in response to pokeweed mitogen, a t-cell-dependent b-cell activator, was depressed. experiments with cultured lym ...	1981	6270560
establishment and characterization of permanent cell lines from patients with acute and hairy cell leukemia.	four cell lines were established from peripheral blood of patients with leukemia. all lines express the epstein--barr virus nuclear antigen (ebna) and therefore should be classified as lymphoblastoid cell lines. however, one of the lines uhkt-5 established from a patient with acute myelomonocytic leukemia has some features not typical for lymphoblastoid cell lines. the cells resemble to macrophages with the phagocytic ability for yeasts, strong alpha-naphthylacetate esterase positivity in phagoc ...	1981	6270579
epstein-barr virus infection and neoplasia.	once acquired, epstein-barr virus (ebv), a latent virus, remains in the body for what appears to be the lifetime of the human host. circumstantial data suggest ebv is involved in clinical disease including malignancies far more often than previously recognized. a serologic test for early antigen (ea) is more specific for diagnosing active ebv disease than the monospot or heterophile test. a case study of active epstein-barr infection is reported showing persistently elevated early antigen titers ...	1981	6270614
the immune system in slow disease due to persistent submicrobial (viral) infection.		1981	6270625
analysis of restriction endonuclease fragments of intracellular epstein-barr virus dna and isoenzymes indicate a common origin of the raji, nc-37, and f-265 human lymphoid cell lines.		1981	6270894
[burkitt's lymphoma].		1981	6271024
etiological implications on kaposi's sarcoma.	in attempts to encompass multiple events responsible for the development of cancer in man, with a particular interest to the possible involvement of viruses, multidisciplinary studies have been conducted on ks during 1971-1979. there is accumulating evidence associating this malignancy with cmv. african ks patients have high antibody titers to cmv, but they are not significantly different from controls of the same regions (ag-ab complexes?). a specific serologic association of european and ameri ...	1981	6271042
different populations of rheumatoid factor idiotypes induced by two polyclonal b cell activators, pokeweed mitogen and epstein--barr virus.		1981	6271428
variant translocation in a non endemic case of burkitt's lymphoma: t (8;22) in an epstein--barr virus negative tumour and in a derived cell line.		1981	6271555
cytotoxic t cell recognition of epstein-barr virus-infected b cells. ii. blocking studies with monoclonal antibodies to hla determinants.	monoclonal antibodies specifically binding common determinants on all hla-a, b anc c antigen molecules blocked the lysis of eb virus-transformed target cells by eb virus-specific cytotoxic t cells reactivated in vitro. blocking was mediated through binding of the antibodies to the target rather than to the effector cells and was maximal (75 to 85% inhibition of lysis) at saturating antibody concentrations. a similar blocking effect was also shown by a monoclonal antibody binding to beta 2-microg ...	1981	6271557
a microassay for quantitatively detecting the epstein-barr virus receptor on single cells utilizing flow cytometry.	a quantitative microassay for detecting and analyzing the epstein-barr virus receptor (ebvr) utilizing fluorescein-conjugated virions is presented. the test is virus substrain-specific. both the b95-8 and p3hr-1 strains were labelled and adsorbed to a variety of targets. relative binding of virus was assessed by flow cytometry, the results being directly comparable with those obtained by earlier methods. cell size and cellular dna content were measured simultaneously with virus binding, thus ena ...	1981	6271799
impaired regulation of epstein-barr virus-induced lymphocyte proliferation in rheumatoid arthritis is due to a t cell defect.	the rate of outgrowth of ebv-infected b lymphocytes is regulated by normal t lymphocytes. removal of t cells from normal whole lymphoid populations (pbm) markedly shortens the outgrowth time of the remaining b lymphocytes. there is little difference in the much more rapid outgrowth of rheumatoid pbm after the removal of t cells, which suggests that ra lymphoid cells are unable to regulate this process. to determine whether ra t cells are defective, or ebv-infected ra b cells are unresponsive to ...	1981	6271870
ig biosynthesis in a human pre-b cell line.	ig synthesis was investigated in a human epstein-barr virus transformed pre-b-like lymphoblastoid line, josh 4. translation of total extracted cellular rna in a wheat germ cellfree system showed that the transcripts for both micrometer (the heavy chain of membrane igm) and micros (the heavy chain of secreted igm) were present in approximately equal proportions. pulse-labeling of cells with 35s-methionine in culture followed by immunoprecipitation and endo-n-acetylglucosaminidase digestion of the ...	1981	6271874
abnormal lymphocyte subsets of x-linked lymphoproliferative syndrome.		1981	6271875
the regulated expression of epstein-barr virus. iii. proteins specified by ebv during the lytic cycle.	the experiments show that 30 virus-induced or virus-specified proteins were synthesized in raji cells after superinfection with epstein-barr virus (ebv) derived from p3hr1 cells. using a combination of pulse labelling, application of cycloheximide blocks at different times post-infection, treatment with amino acid analogues and inhibition of dna synthesis it was shown that three groups of proteins appear in raji cells after superinfection; the synthesis of the proteins in any one group appears t ...	1981	6271909
oncogenic viruses.		1981	6271940
on the dynamics of abrin binding to receptor sites in normal and epstein barr virus transformed lymphocyte cell cultures.	the effects of treatment with varying doses of abrin, a d-galactose binding lectin, on dna an protein synthesis of normal and epstein barr virus transformed lymphocytes has been investigated. activation, stimulation, and relative toxicity factor indices are studied, as well as possible relationships between dna and protein synthesis rates, as measured by simultaneous tritiated thymidine (3h-tdr) and 14c-leucine uptake. studies of the two new indices, the metabolic self and cross coupling indices ...	1981	6271962
analysis of the transformation of human lymphocytes by epstein-barr virus iii. induction of early events by p3hr-1 strain without subsequent immortalization.	t-cell depleted human cord blood lymphocytes were exposed to p3hr-1 strain of epstein-barr virus (ebv) and simultaneous observations of immunofluorescence, cellular morphology, and autoradiography were carried out in individual cells. soon after infection, nuclear antigen (ebna) synthesis, blastogenesis, and dna synthesis occurred, as was previously observed b95-8 strain ebv infection. although mitosis followed with characteristic cell aggregate formation, the cell proliferation was temporary an ...	1981	6272076
retinoid-induced growth inhibition of herpesvirus-transformed marmoset lymphoblastoid cell lines.	the in vitro proliferation of herpesvirus-transformed marmoset lymphoblastoid cell lines (lcl) was inhibited by retinoic acid and retinyl acetate. both herpesvirus-ateles-and herpesvirus-saimiri-transformed lcl with t-cell characteristics and epstein-barr-transformed lcl with b-cell markers were more sensitive to retinoic acid than to retinyl acetate. inhibition of lcl proliferation was dependent on retinoid concentration and became apparent only after 3-4 days of exposure. in vitro assays for m ...	1981	6272171
a new view of the etiology of nasopharyngeal carcinoma.		1981	6272265
[seroepidemiologic studies in lymphogranulomatosis].		1981	6272267
pleiotropic expression of epstein--barr virus dna in human epithelial cells.	we have attempted to establish a system that can be used to study the association of epstein--barr virus (ebv) with epithelial cells. attempts were made to transfect human carcinoma cells with ebv dna. successful transfection was confirmed by the expression of ebv-specific early antigen (ea), virus capsid antigen, and the presence of virus dna. the transfecting preparation contained a mixture of ebv and cellular dna extracted from two producer cell lines, p3hr-1 and ag-876. our data suggest that ...	1981	6272321
transfusion transmitted diseases.		1981	6272359
serological follow-up of children with infectious mononucleosis caused by epstein-barr virus.	ninety children, most under 6 years of age, 73 of whom were suffering from a disease suspect of infectious mononucleosis, were examined serologically for ebv infection. acute ebv infection was demonstrated in 40 cases. these children were followed up until the end of the second year by examining serum samples taken every month of every third month for anti-vca-igm, anti-vca-igg, anti-vca-iga anti-ebna, and for the specific antibodies to the components d and r of the early antigen. heterophil ant ...	1981	6272540
ocular infectious mononucleosis manifested as parinaud's oculoglandular syndrome.		1981	6272577
characterization of the epstein-barr virion-associated dna polymerase as isolated from superinfected and drug-stimulated cells.	we reported previously that epstein-barr (eb) virions and detergent-treated nucleocapsids co-purified with significant amounts of dna polymerase activity that did not resemble other known host or viral polymerases. we report here that this species of dna polymerase activity is present at early times after infection in lymphocytes abortively lytically infected (superinfected) with eb virus. however, studies with [35s]methionine labeling suggest de novo synthesis of enzyme has not occurred. conver ...	1981	6272863
somatic cell hybrid analyses of hematopoietic differentiation.	a differentiated cell expresses an entire set of specialized features. somatic cell hybridization provides a method to examine control of gene regulation. we studied the expression of tissue-specific features in hybrids between human promyelocytes (hl-60) and human burkitt's lymphoma cells (p3hr-1). two hybrid lines, hp-1 and hp-2, and 18 hybrid clones were established and confirmed by karyotype, isozyme, and surface antigen analyses. the hybrids extinguished the 10 myeloid (hl-60) features that ...	1981	6272904
epstein-barr virus antibodies in patients with ataxia-telangiectasia and other immunodeficiency diseases.	an unusual antibody response to the epstein-barr virus (ebv) has been noted in patients with ataxia-telangiectasia. of a group of 16 such patients 8 were found to have antibodies in their serum to the ebv viral capsid antigen (vca), and 4 of them also had antibodies to the ebv early antigen (ea); antibodies to the nuclear antigen (ebna), however, were absent in 3 of the 8. the antibody pattern persisted for more than 2 years in the patients available for follow-up study. in comparison, of 24 pat ...	1981	6272957
a short-term in vitro assay for promoter substances using human lymphoblastoid cells latently infected with epstein-barr virus.	we designed a short-term in vitro assay for detecting tumor promoters, utilizing the activation of epstein-barr virus (ebv) expression in ebv genome-carrying human lymphoblastoid cells. this system is composed of ebv-non-producer raji cells as the indicator, n-butyrate as the ebv-inducer, and the test substance. after addition of the latter 2 components to the culture medium, the cells are cultivated for 48 h at 37 degrees c and the ratio of ebv early antigen (ea)-expressing cells was assessed u ...	1981	6272961
enhancement of epstein-barr virus-induced transformation of human lymphocytes by teleocidin.		1981	6272962
introduction to epstein-barr virus and lymphoproliferative diseases in immunodeficient individuals.		1981	6272963
t-cell response to b-cells and epstein-barr virus antigens in infectious mononucleosis.	after epstein-barr virus (ebv) infection in vivo, b-cells with latent virus infection persist indefinitely through life. these cells grow in vitro on explanation and can be established as immortal b-cell lines. to reconcile the unlimited growth potential in vitro with the maintenance of a low proportion of b-cells infected by ebv in vivo, a strict in vivo control mechanism has to be postulated. certain aspects of this control are apparent when the primary infection is followed by infectious mono ...	1981	6272964
long-term t-cell-mediated immunity to epstein-barr virus.		1981	6272965
epstein-barr virus-specific serology in immunologically compromised individuals.	since b-lymphocytes are targets and a continuing habitat of epstein-barr virus (ebv) and the cell-mediated immune system becomes secondarily involved, one may anticipate that primary and persistent ebv infections in immunologically compromised individuals take unusual courses. depending on the immunological defect, the clinical, hematological, and serological responses to primary ebv infections may be more or less pronounced than in immunologically competent patients. infectious mononucleosis ha ...	1981	6272966
documentation of epstein-barr virus infection in immunodeficient patients with life-threatening lymphoproliferative diseases by clinical, virological, and immunopathological studies.	multiple methods, pedigree analysis, clinical evaluation, and epstein-barr virus (ebv)-specific serology, ebv dna hybridization of tissues to probe for viral genome, staining of touch imprints for ebv nuclear-associated antigen, establishment of spontaneous infected b-cell lines from peripheral blood or tissues, examination of peripheral blood smears, and hematopathology studies, were used to study seven patients with the x-linked lymphoproliferative syndrome and seven additional patients with l ...	1981	6272967
documentation of epstein-barr virus infection in immunodeficient patients with life-threatening lymphoproliferative diseases by epstein-barr virus complementary rna/dna and viral dna/dna hybridization.	tissues from patients thought to have epstein-barr virus (ebv)-induced lymphoproliferative diseases were probed for ebv genomes using 2 independent hybridization techniques. tissues from six patients with the x-linked lymphoproliferative syndrome, all five renal allograft recipients with immunoblastic sarcoma, and eight patients with diverse types of immunodeficiency and lymphoproliferative diseases such as fatal infectious mononucleosis or malignant lymphoma associated with antecedent immunodef ...	1981	6272968
epstein-barr virus in a malignant lymphoproliferative disorder of b-cells occurring after thymic epithelial transplantation for combined immunodeficiency.	a fatal disseminated polyclonal malignant lymphoproliferative disorder of b-cells (immunoblastic sarcoma) developed shortly after a second thymic epithelial peritoneal implant in a 5-yr-old girl with combined immunodeficiency. the immunodeficiency was characterized by low t-cell numbers and function, very low levels of thymic hormone, dysgammaglobulinemia, and an inability to mount a primary antibody or cell-mediated response to new antigens. at necropsy, the thymus fulfilled morphological crite ...	1981	6272969
diverse familial malignant tumors and epstein-barr virus.	continued monitoring of a family for new malignant tumors has revealed diverse immunological and neoplastic disorders during a 15-year period. in 1966, the proband developed lymphoma. in 1975, his antibody titers to epstein-barr virus (ebv) became elevated, and again, he developed a malignant lymphoma. he also had borderline hypo-immunoglobulin a, died of glioblastoma multiforme in 1977, and at autopsy, had adenomatous colonic polyps. his eldest brother has normal immunoglobulin levels, but deve ...	1981	6272970
clinical spectrum of lymphoproliferative disorders in renal transplant recipients and evidence for the role of epstein-barr virus.	six renal transplant recipients with abnormal lymphoproliferative disorders were studied in an attempt to define their clinical features and the role of epstein-barr virus (ebv) in their pathogenesis. patients were either teenage (three) or in the sixth decade (three). the younger patients presented an average of 3 months after transplantation with fever, sore throat, and lymphadenopathy; had been markedly immunosuppressed; frequently had preceding or concomitant cytomegalovirus infections; and ...	1981	6272971
fatal epstein-barr virus infection in a child with acute lymphoblastic leukemia in remission.	a 9-year-old white boy developed a fatal primary epstein-barr virus (ebv) infection while receiving chemotherapy for acute lymphoblastic leukemia in remission. histopathological findings at the height of the proliferative phase of the illness were compatible with a virally induced hemophagocytic syndrome. the infection spontaneously converted to complete aplasia of the bone marrow and lymph nodes. serological studies disclosed that the patient had no antibodies to ebv prior to the infection, but ...	1981	6272972
cell-mediated immune reactions in three patients with malignant lymphoproliferative diseases in remission and abnormally high epstein-barr virus antibody titers.	two patients with hodgkin's disease in remission and one chronic lymphatic leukemia patient with extraordinarily high anti-epstein-barr virus (ebv) (viral capsid antigen) antibody titers (greater than 10,000) were selected to study a spectrum of cell-mediated immune responses, including natural killer, interferon-boosted killer, antibody-dependent lymphocytotoxicity, and t-cell-mediated reactions. the purpose was to compare these reactions in patients with immunosuppression and a high ebv load w ...	1981	6272973
summary: symposium on epstein-barr virus-induced lymphoproliferative diseases in immunodeficient patients.		1981	6272974
g1-phase arrest of cultured human leukemic t-cells induced by deoxyadenosine.	cultured human t-cell leukemia lymphocytes have enhanced sensitivity to growth inhibition by deoxyadenosine. we have used flow cytometry to investigate the mechanism of deoxyadenosine toxicity in cultured t-leukemic cells. comparative studies on deoxyadenosine-resistant epstein-barr virus-transformed b-lymphocyte cell lines were also performed. after exposure of t-cells to low concentrations of deoxyadenosine (3 microm), in the presence of an adenosine deaminase inhibitor (erythro-9-[3-(2-hydrox ...	1981	6272990
inhibitory effect of 1-beta-d-arabinofuranosylthymine on synthesis of epstein-barr virus.	the effect of 1-beta-d-arabinofuranosylthymine (ara-t) on cell growth and synthesis of epstein-barr virus (ebv) in human lymphoblastoid cell lines was determined. the growth of p3hr-1 cells was not inhibited by 1 microgram of the drug per ml; however, infectious virus production was strongly inhibited and was accompanied by decreased expression of early antigen (ea) and viral capsid antigen (vca). the ability of 12-o-tetradecanoylphorbol-13-acetate or n-butyric acid to induce synthesis of vca, b ...	1981	6268944
production of infectious epstein--barr virus in mouse lymphocytes.		1981	6268991
[epstein-barr virus infection in the development of human tumors].	the epstein-barr virus (ebv) is the only infectious agent for which a close association with human malignant tumors has been clearly demonstrated. these tumors are one type of nasopharyngeal carcinoma which is frequent in parts of east asia and the burkitt lymphoma which predominantly occurs in parts of africa and new guinea. nonetheless, the ebv is the causative agent of infectious mononucleosis (im), a benign, self-limiting lymphoproliferative disease of adolescents. the major difference betwe ...	1981	6268993
molecular cloning of the complete epstein-barr virus genome as a set of overlapping restriction endonuclease fragments.	a complete collection of fragments of epstein-barr virus dna, obtained by cleavage with restriction endonuclease eco ri, has been cloned. fourteen different internal fragments of the virus genome, derived from linear virion dna of the b95-8 strain, and sequences corresponding to the terminal regions of virion dna, derived from intracellular circular ebv dna isolated from 895-8 cells, were cloned. sizes of fragments were determined by agarose gel electrophoresis and their sum leads to an estimate ...	1981	6269068
cyclosporin a and steroid therapy in sixty-six cadaver kidney recipients.	from nine to 18 months ago, 66 patients were given 67 randomly matched cadaveric kidneys with cyclosporin a and steroid therapy. nine of the recipients were undergoing retransplantation. the over-all kidney survival rate to date has been 77.6 per cent, and 78.8 per cent of the recipients are dialysis-free. the patient mortality in this learning phase was 13.3 per cent. nephrotoxicity, hepatotoxicity and other side-effects of cyclosporin a could usually be dealt with by dosage adjustments, making ...	1981	6269238
mechanism of inhibition of epstein-barr virus replication by phosphonoformic acid.		1981	6269292
immune deficiency predisposing to epstein-barr virus-induced lymphoproliferative diseases: the x-linked lymphoproliferative syndrome as a model.		1981	6269372
characterization of purified epstein--barr virus induced deoxyribonucleic acid polymerase: nucleotide turnover, processiveness, and phosphonoacetic acid sensitivity.	the epstein--barr (eb) virus induced dna polymerase has been further purified and characterized with respect to nucleotide turnover activity, processiveness of synthesis, and interaction with phosphonoacetic acid (paa). the polymerase as purified through denatured dna--cellulose chromatography was inseparable from a labile nuclease activity associated with an equally labile dna-dependent nucleotide turnover function. the eb virus induced dna polymerase even in the absence of detectable nuclease ...	1981	6269579
in vitro blastogenic responses of peripheral lymphocytes from marmosets to phytohemagglutinin and to autologous lymphocytes transformed by epstein-barr virus.		1981	6269800
epstein-barr virus and human disease: immune responses determine the clinical and pathologic expression.		1981	6269987
atypical lymphocytosis in children.	a complete blood count performed during the evaluation of a febrile or traumatized child may show the presence of atypical lymphocytosis. although atypical lymphocytes occur in several conditions, they are most closely associated with infectious mononucleosis (im). we initiated a study to determine if the presence in children of atypical lymphocytosis without the triad of splenomegaly, pharyngitis, and adenopathy is a manifestation of im. heterophil antibody (ha) and epstein-barr virus (ebv) tit ...	1981	6266286
effect of novobiocin on dna synthesis and structure in human lymphoblastoid cells.		1981	6266406
separation and properties of molecular forms of alpha-galactosidase and alpha-n-acetylgalactosaminidase from blood lymphocytes and lymphoid cell lines transformed by epstein-barr virus.		1981	6266491
epstein-barr virus co-reconstituted with sendai virus envelopes infects epstein-barr virus-receptor negative cells.	epstein-barr virus (ebv) was co-reconstituted with sendai virus envelopes. the reconstituted "hybrid' virus could bind and penetrate into ebv-receptor negative cells. using this approach, t-cell-derived human and mouse leukemia cells, human t-lymphocytes and mouse spleen cells were successfully infected as judged by the induction of ebv-determined antigens and stimulation of dna synthesis. the t-cell-derived human leukemia line molt-4, that can absorb ebv but without virus penetration, could be ...	1981	6266500
chromosomes and causation of human cancer and leukemia. xlv. chromosome patterns in stimulated lymphocytes of chronic lymphocytic leukemia.	in the present study the b-cell mitogens from epstein-barr virus (ebv) and lipopolysaccharide-b from e. coli (lps) were used to stimulate the blood cells of 27 patients with chronic lymphocytic leukemia (cll) to undergo mitosis. in 14 cll cases ebv was used as the only mitotic stimulant and in 5 cases both ebv and lps. in 8 cases neither ebv nor lps caused mitotic cells to appear. in 9 cases the cells were characterized by abnormal clones, including 5 cases with +12 (including 1 case with a poss ...	1981	6266649
combined effect of the extracts from croton tiglium, euphorbia lathyris or euphorbia tirucalli and n-butyrate on epstein-barr virus expression in human lymphoblastoid p3hr-1 and raji cells.	the combined usage of n-butyrate and 12-o-tetradecanoylphorbol-13-acetate (tpa) or the oily extracts from croton tiglium, euphorbia lathyris or euphorbia tirucalli exerted a marked effect on induction of epstein-barr virus (ebv)-associated early (ea) and viral capsid (vca) antigens in ebv genome-carrying human lymphoblastoid cell lines. in producer p3hr-1 cells, the enhancing effect of the 2 components was additive both for ea and vca, while in non-producer raji cells, a synergistic increase of ...	1981	6266651
[levels of epstein-barr virus antibodies in the serum of children with acute lymphoblastic leukemia].		1981	6266677
detection of anti-platelet antibodies in patients with idiopathic thrombocytopenic purpura (itp) and in patients with rubella and herpes group viral infections.	a highly sensitive enzyme-linked immunosorbent assay (elisa) technique was observed for serological detection of antibodies against platelets. a covalently bound to sepharose cl-4b was used to enrich sera in the igg3 subclass of antibodies. the elisa procedure as applied to detect anti-platelet antibodies in patients with herpes or rubella viral infections and in patients with idiopathic thrombocytopenic purpura (itp). twenty-eight sera from 13 thrombocytopenic patients showed high levels of ant ...	1981	6266723
fc and c3 receptor patterns on two ebv- negative burkitt lymphoma lines during acute exposure to ebv (p3hr-1 substrain).		1981	6266834
restriction of foetal calf serum-induced cytotoxicity by human t-cells.		1981	6266949
physiologic concentrations of normal human plasma lipoproteins inhibit the immortalization of peripheral b lymphocytes by the epstein-barr virus.	epstein-barr virus (ebv)-induced immortalization of adult human b lymphocytes is suppressed by physiologic concentrations of human plasma lipoproteins. several inhibitory mechanisms appear to be operative. first, low density lipoproteins (ldl) directly reduce the ability of ebv to transform human b cells. second, ldl as well as intermediate and very low density lipoproteins modulate early inductive events rendering the b cell refractory to transforming signals from ebv. third, ldl also selective ...	1981	6267100
induction of complement receptor expression in cell lines derived from human undifferentiated lymphomas. ii. characterization of the induced complement receptors and demonstration of the simultaneous induction of ebv receptor.	we have studied the specificity of complement receptors induced by theophylline in 2 cell lines derived from undifferentiated lymphomas, one of burkitt's type, and compared it to that of complement receptors in other cell types. both c3b and c3d receptors were induced. the induced c3b receptor differed from the c3b receptor of mature normal lymphocytes, polymorphonuclear leukocytes and the cells of a nodular lymphoma in 2 respects. firstly, it bound c3b much less avidly (by a factor of several h ...	1981	6267130
induction of epstein-barr virus early antigen, virus capsid antigen and virus dna synthesis by mitomycin c.	the effect of mitomycin c was tested on epstein-barr virus (ebv) antigen and virus dna synthesis in producer and non-producer cell lines. we found that producer, but not non-producer cell lines, are inducible for early antigen and virus capsid antigen synthesis. although host dna synthesis is effectively inhibited by over 99% in the presence of mitomycin c, virus dna synthesis in the producer lines is unaffected and induced in parallel with the virus antigens.	1981	6267175
the epstein-barr virus (ebv) in the pathogenesis of posttransplant lymphoma.		1981	6267753
early dna-binding polypeptides of epstein-barr virus.		1981	6267784
protein kinase activity associated with epstein-barr virus-determined nuclear antigen.		1981	6267796
leukocyte response in childhood infectious mononucleosis. caused by epstein-barr virus.	the usual leukocyte response in infectious mononucleosis (im) has been established in adults but not in children. previously, the diagnosis of im often was difficult in childhood due to the lack of detectable heterophil antibody response in many patients. using epstein-barr virus-specific serology, we have documented im in 45 patients between 1 and 15 years of age. the mean and ranges for the total leukocytes, percent lymphocytes, and percent atypical lymphocytes are presented. these do not vary ...	1981	6267933