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postresuscitation myocardial diastolic dysfunction following prolonged ventricular fibrillation and cardiopulmonary resuscitation.postresuscitation myocardial dysfunction is one of the leading causes of early death after successful resuscitation from sudden death. however, the diastolic characteristics of postresuscitation myocardial dysfunction are not well defined. we therefore investigated the postresuscitation left ventricular diastolic function following prolonged cardiac arrest and subsequent cardiopulmonary resuscitation.200818090365
effects of ventilation with 100% oxygen during early hyperdynamic porcine fecal peritonitis.early goal-directed therapy aims at balancing tissue oxygen delivery and demand. hyperoxia (i.e., pure oxygen breathing) has not been studied in this context, since sepsis increases oxygen radical production, which is believed to be directly related to the oxygen tension. on the other hand, oxygen breathing improved survival in various shock models. therefore, we hypothesized that hyperoxia may be beneficial during early septic shock.200818091553
a new device to simplify flexible endoscopic treatment of zenker's diverticulum.endoscopic treatment of a zenker's diverticulum (zd) is challenged by maintaining a stable position and visual field. an oblique transparent cap and an overtube have been used with success. the porcine hypopharyngeal pouch is similar to a zd. while using the widespread emr device (wemr) in the esophagus, we realized that the design may be advantageous for treatment of a zd.200818155432
radiofrequency hepatic ablation with internally cooled electrodes and hybrid applicators with distant saline infusion using an in vivo porcine model.radiofrequency ablation (rfa) of tumors by means of internally cooled (ice) or multitined expandable electrodes combined with infusion of saline into the tissue may improve results. our aim was to determine the efficacy of a previously optimized hybrid ice system (ice combined with infusion of saline into the tissue at a distance of 2mm) in comparison with a conventional ice cluster electrode in porcine liver in vivo.200818042500
immunoglobulin light chain (igl) genes in zebrafish: genomic configurations and inversional rearrangements between (v(l)-j(l)-c(l)) gene clusters.in mammals, immunoglobulin light chain (igl) are localized to two chromosomal regions (designated kappa and lambda). here we report a genome-wide survey of igl genes in the zebrafish revealing (v(l)-j(l)-c(l)) clusters spanning 5 separate chromosomes. to elucidate igl loci present in the zebrafish genome assembly (zv6), conventional sequence similarity searches and a novel scanning approach based on recombination signal sequence (rss) motifs were applied. rt-pcr with zebrafish cdna was used to c ...200818022691
a lightweight, partially absorbable mesh (ultrapro) for endoscopic hernia repair: experimental biocompatibility results obtained with a porcine model.a meticulous surgical technique, a mesh of adequate dimensions, and use of a mesh with good biocompatibility properties are decisively important for minimizing the development of recurrences after endoscopic hernia repair surgery. mesh "shrinkage" is a function of the mesh's biocompatibility, that is, the properties of the mesh. large-pore, lightweight polypropylene meshes possess the best biocompatibility, and the newly developed meshes meet these requirements.200817963002
indomethacin decreases optic nerve oxygen tension by a mechanism other than cyclo-oxygenase inhibition.we investigated the effect of several non-steroidal anti-inflammatory drugs (nsaids), on the preoptic nerve oxygen tension (onpo2), as indomethacin previously has demonstrated a strong decreasing effect on onpo2. we tested whether these nsaids, like indomethacin, also reduce the increasing effect of dorzolamide on onpo2.200817965100
use of a chitosan-based hemostatic dressing in laparoscopic partial nephrectomy.an external chitosan-based hemostatic bandage has been used to control aggressive bleeding from traumatic injuries. this study was to evaluate the feasibility of using an internal chitosan dressing based on the external platform to control hemorrhage and urinary leakage by sealing off the parenchymal wound following laparoscopic partial nephrectomy (lpn) in a porcine model.200817932955
validation of two commercial real-time rt-pcr kits for rapid and specific diagnosis of classical swine fever virus.two real-time rt-pcr kits, developed by lsi (taqvet csf) and adiagene (adiavet csf), obtained an agreement to be commercialised in france, subject to conditions, defined by the french classical swine fever (csf) national reference laboratory. the producers were asked to introduce an internal control to check the rna extraction efficacy. the different criteria assessed were sensitivity, "pestivirus specificity", reproducibility and ease of handling, using 189 different samples. these samples were ...200817913249
intravenous tezosentan improves gas exchange and hemodynamics in acute lung injury secondary to meconium aspiration.meconium aspiration induces acute lung injury (ali) and subsequent pulmonary arterial hypertension (pah) which may lead to right ventricular failure. increase of endothelin-1, thromboxane-a, and phosphodiesterases are discussed molecular mechanisms. we investigated the intrapulmonary and hemodynamic effects of the intravenous dual endothelin a and b receptor blocker tezosentan and inhalational iloprost in a model of ali due to meconium aspiration.200817898997
cryopreservation of the germplasm of animals used in biological and medical research: importance, impact, status, and future directions.molecular genetics and developmental biology have created thousands of new strains of laboratory animals, including rodents, drosophila, and zebrafish. this process will accelerate. a decreasing fraction can be maintained as breeding colonies; hence, the others will be lost irretrievably unless their germplasm can be cryopreserved. because of the increasingly critical role of cryopreservation, and because of wide differences in the success with which various forms of germplasm can be cryopreserv ...200817901073
alkali-labile sites in sperm cells from sus and ovis species.constitutive alkali-labile sites (alss) have been investigated using a protocol of dna breakage detection-fluorescence in situ hybridization (dbd-fish) in sperm cells from sus domesticus (pig), ovis gmelini musimon (mouflon) and ovis aries (sheep). the results were compared with those obtained using leucocytes from the same species. whole comparative genomic hybridization (w-cgh) showed that most of the constitutive alss in somatic and germ line cells in all species examined were constrained to ...200817651406
histopathological classification of systemic mycobacterium avium complex infections in slaughtered domestic pigs.the aim of this study was to classify the histopathological features of pigs infected with mycobacterium avium complex (mac). we used slaughtered pig organs systemically infected with mac. the results showed granulomatous lesions which were observed predominantly in the digestive organs and regional lymph nodes rather than respiratory organs. the histological picture showed a wide range of granulomatous stages from exudative to fibrotic reactions to the mac infection. eosinophils and giant cells ...200817629560
photoperiod and luteinizing hormone secretion in domestic and wild pigs.during seasonal anoestrus (long-days), oestradiol can effectively inhibit the pulsatile secretion of luteinizing hormone (lh) in sheep. the aim of our trial was to determine whether the same regulatory mechanism exists in the pig. altogether, 20 ovariectomized and oestradiol-implanted gilts (16 domestic pigs, 4 european wild boars) were randomly allocated to two treatment groups. the first group was kept under a short-day light-dark cycle of 8l:16d, and the second group under a long-day light re ...200817250979
a review of viral diseases of the european wild boar: effects of population dynamics and reservoir rôle.there has been a worldwide increase in the number and geographical spread of wild boar populations in recent decades leading to an increase in both the circulation of disease agents and greater contact with domestic animals and humans. diseases affect the population dynamics of wildlife but the effects of most viral diseases on the european wild boar are largely unknown. many viral diseases present in domestic pig populations are also present in wild boars where they can provide a disease reserv ...200817420149
laser-induced thermotherapy for lung tissue--evaluation of two different internally cooled application systems for clinical use.thermal ablation techniques like radiofrequency or laser-induced thermotherapy (litt) are increasingly used to treat tumors of parenchymatous organs. minimal access, parenchymal preservation, and a low complication rate render them suitable for pulmonary tumors as well. their successful clinical application depends on the induction of sufficiently large lesions and a knowledge of the energy parameters required for complete thermal ablation. the aim of this study was to establish a dose-response ...200817599236
current world status of balantidium coli.balantidium coli is a cosmopolitan parasitic-opportunistic pathogen that can be found throughout the world. pigs are its reservoir hosts, and humans become infected through direct or indirect contact with pigs. in rural areas and in some developing countries where pig and human fecal matter contaminates the water supply, there is a greater likelihood that balantidiosis may develop in humans. the infection may be subclinical in humans, as it mostly is in pigs, or may develop as a fulminant infect ...200818854484
serial noninvasive in vivo positron emission tomographic tracking of percutaneously intramyocardially injected autologous porcine mesenchymal stem cells modified for transgene reporter gene expression.porcine bone marrow-derived mesenchymal stem cells (mscs) were stably transfected with a lentiviral vector for transgene expression of the trifusion protein renilla luciferase, red fluorescent protein and herpes simplex truncated thymidine kinase (lv-rl-rfp-ttk; positron emission tomography [pet] reporter gene) for in vivo noninvasive tracking of the intramyocardially delivered msc fate.200819808526
expression, cellular localization and antibody responses of the african swine fever virus genes b602l and k205r.previously, we identified serological immunodeterminants of african swine fever virus (asfv), including pk205r and pb602l, without homologues in the database. pk205r is expressed as a 33-kd protein from 4 h post-infection onward, initially diffusely distributed throughout cells, and subsequently in viral factories. pk205r was not found in purified virus. both pk205r and pb602l are recognised by hyperimmune antisera from domestic pigs and bushpigs at late time points after infection, suggesting t ...200819011730
transvenous intramyocardial cellular delivery increases retention in comparison to intracoronary delivery in a porcine model of acute myocardial infarction.clinical trials using intracoronary (ic) delivery of cells have addressed efficacy but the optimal delivery technique is unknown. our study aimed to determine whether transvenous intramyocardial (tvim) approach was advantageous for cellular retention in ami.200819012733
the adult göttingen minipig as a model for chronic heart failure after myocardial infarction: focus on cardiovascular imaging and regenerative therapies.porcine models have become increasingly popular in cardiovascular research. the standard farm pig rapidly increases in body weight and size, potentially confounding serial measurements of cardiac function and morphology. we developed an adult porcine model that does not show physiologic increases in heart mass during the study period and is suitable for long-term study. we compared adult minipigs with the commonly used adolescent yorkshire swine. myocardial infarction was induced in adult göttin ...200819149414
bama miniature pigs (sus scrofa domestica) as a model for drug evaluation for humans: comparison of in vitro metabolism and in vivo pharmacokinetics of lovastatin.the objective of this study was to demonstrate that bama miniature pigs are a suitable experimental animal model for the evaluation of drugs for man. to this end, in vitro lovastatin metabolism at the minipig liver microsomal level and in vivo pharmacokinetics were studied. results were compared with those obtained from humans. our data indicate that the main metabolites and enzyme kinetic parameters of lovastatin metabolism are similar in pigs and humans. triacetyloleandomycin, a specific inhib ...200819149415
development of a recombinant antigen-based elisa for the sero-detection of porcine lymphotropic herpesviruses.xenotransplantation using pigs as donor species carries a risk for the activation of latent porcine herpesviruses and potential transmission to the human recipient. the porcine lymphotropic herpesviruses (plhv-1, -2, -3) are widespread in domestic pigs and closely related to the human gammaherpesviruses, epstein-barr virus and kaposi sarcoma herpesvirus, causing lymphoproliferative disorders. plhv-1 has been associated with a porcine post-transplantation lymphoproliferative disorder (ptld), affe ...200819152663
expression of vascular endothelial growth factor receptors coincide with blood vessel in-growth and reactive bone remodelling in experimental intervertebral disc degeneration.to analyze immunohistochemically the localization of the vegf receptors in experimental intervertebral disc degeneration tissues in a pig model.200819210865
intraindividual comparison of image quality in mr urography at 1.5 and 3 tesla in an animal model.experimental evaluation of image quality of the upper urinary tract in mr urography (mru) at 1.5 and 3 tesla in a porcine model.200819238642
modulation of porcine (sus scrofa domestica) and pheasant (phasianus colchicus) carbonyl reducing enzymes by anthelmintic therapy with flubendazole.flubendazole (flu) is a widely administered benzimidazole anthelmintic indicated for the control of parasitic diseases in farm animals including pigs and pheasants. this study was designed to test the biotransformation of flu in control animals and animals treated with flu in recommended therapeutic doses. the activities of several pheasant and porcine hepatic and intestinal carbonyl reducing enzymes and their modulation by flu were also studied. twelve adult pheasant hens, approximately 1 year ...200819356067
the amplitude spectrum area correctly predicts improved resuscitation and facilitated defibrillation with head cooling.when systemic hypothermia was maintained before inducing cardiac arrest, the likelihood of successful defibrillation and meaningful survival was increased. when hypothermia is induced during cardiopulmonary resuscitation, mortality is also improved. with the introduction of the amplitude spectrum area as a predictor of the success of electrical defibrillation, we investigated the effect of preferential head cooling initiated coincident with cardiopulmonary resuscitation on amplitude spectrum are ...200820449903
a comparison between head cooling begun during cardiopulmonary resuscitation and surface cooling after resuscitation in a pig model of cardiac arrest.employing transnasal head-cooling in a pig model of prolonged ventricular fibrillation, we compared the effects of 4 hrs of head-cooling started during cardiopulmonary resuscitation with those of 8 hrs of surface-cooling started at 2 hrs after resuscitation on 96-hr survival and neurologic outcomes.200820449906
intra-arrest rapid head cooling improves postresuscitation myocardial function in comparison with delayed postresuscitation surface cooling.to compare resuscitation outcomes and myocardial function among intra-arrest head cooling, delayed surface cooling, and uncooled controls.200820449907
neuroprotective effects of the inhalational anesthetics isoflurane and xenon after cardiac arrest in pigs.neurologic outcome after cardiopulmonary resuscitation from cardiac arrest carries a poor prognosis and treatment options to ameliorate brain damage are limited.200820449916
traditional zootherapeutic studies in india: a review.the present study aims to review the zootherapeutic practices of the different ethnic communities of india. this work is also an attempt to present a list of animals' use for medicinal purposes by different communities of india. data were gathered from 15 published research papers of various authors on zootherapeutic studies in india from 2000 to 2007. approximately 109 animals and their 270 uses are reported in traditional medicine in different parts of india. of these, the highest numbers of a ...200818634551
differentiation of european wild boar (sus scrofa scrofa) and domestic swine (sus scrofa domestica) meats by pcr analysis targeting the mitochondrial d-loop and the nuclear melanocortin receptor 1 (mc1r) genes.this work describes the differentiation of european wild boar (sus scrofa scrofa) and domestic swine (sus scrofa domestica) meats by pcr targeting sequences from two molecular markers: the mitochondrial displacement loop (d-loop) region and the nuclear melanocortin receptor 1 (mc1r) gene. a polymorphic d-loop fragment (~270bp) was amplified and sequenced in a number of wild and domestic sus scrofa meat samples, to find a nucleotide region suitable for pcr-rflp analysis. sequence data showed the ...200822062284
volumetric tracking tool using four-dimensional ct for image guided-radiation therapy.to analyze lung vessel motion automatically, we developed computerized tracking software for multi-slice ct (msct) data. here, we evaluated the tracking accuracy in images of healthy porcine lungs obtained by 256 multi-slice ct (256-msct). a domestic pig was scanned with 256-msct under free breathing conditions. the 256-msct provides 128-mm scan coverage with a 0.5-mm slice thickness in a single rotation (1.0 s/rot). automatic tracking was performed by template matching, where volumes of interes ...200820821162
physiologic compliance in engineered small-diameter arterial constructs based on an elastomeric substrate.compliance mismatch is a significant challenge to long-term patency in small-diameter bypass grafts because it causes intimal hyperplasia and ultimately graft occlusion. current engineered grafts are typically stiff with high burst pressure but low compliance and low elastin expression. we postulated that engineering small arteries on elastomeric scaffolds under dynamic mechanical stimulation would result in strong and compliant arterial constructs. this study compares properties of engineered a ...200919962188
the risk of extrapolation in neuroanatomy: the case of the mammalian vomeronasal system.the sense of smell plays a crucial role in mammalian social and sexual behaviour, identification of food, and detection of predators. nevertheless, mammals vary in their olfactory ability. one reason for this concerns the degree of development of their pars basalis rhinencephali, an anatomical feature that has been considered in classifying this group of animals as macrosmatic, microsmatic or anosmatic. in mammals, different structures are involved in detecting odours: the main olfactory system, ...200919949452
the genome sizes of megabats (chiroptera: pteropodidae) are remarkably constrained.it has long been recognized that bats and birds contain less dna in their genomes than their non-flying relatives. it has been suggested that this relates to the high metabolic demands of powered flight, a notion that is supported by the fact that pterosaurs also appear to have exhibited small genomes. given the long-standing interest in this question, it is surprising that almost no data have been presented regarding genome size diversity among megabats (family pteropodidae). the present study ...200919324635
effect of betaine supplementation on power performance and fatigue.the purpose of this study was to examine the efficacy of 15 days of betaine supplementation on muscle endurance, power performance and rate of fatigue in active college-aged men.200919250531
comparative snp diversity among four eucalyptus species for genes from secondary metabolite biosynthetic pathways.there is little information about the dna sequence variation within and between closely related plant species. the combination of re-sequencing technologies, large-scale dna pools and availability of reference gene sequences allowed the extensive characterisation of single nucleotide polymorphisms (snps) in genes of four biosynthetic pathways leading to the formation of ecologically relevant secondary metabolites in eucalyptus. with this approach the occurrence and patterns of snp variation for ...200919775472
identification of mitochondrial markers for genetic traceability of european wild boars and iberian and duroc pigs.iberian pigs and wild boars are the source of highly priced meat and dry-cured products. iberian maternal origin is mandatory for labeled iberian products, making necessary the authentication of their maternal breed origin. discrimination between wild and domestic pig maternal origin may be useful to distinguish labeled wild boar meat obtained from hunting or farming. in order to detect useful polymorphisms to trace iberian, duroc and wild boar maternal lineages, we herein investigated the compl ...200922444897
epicardial ablation on the beating heart: limited efficacy of a novel, cooled radiofrequency ablation device.objective: to perform a minimally invasive procedure for atrial fibrillation without cardiopulmonary bypass, it is necessary to create transmural lesions on the beating heart. although bipolar radiofrequency clamps can isolate the pulmonary veins, they have difficulty in performing any other left atrial lesions, particularly those of the traditional cox-maze procedure. this study examined the performance of an internally cooled, bipolar radiofrequency device designed for such an application. met ...200922323899
mechanical transmission of vesicular stomatitis new jersey virus by simulium vittatum (diptera: simuliidae) to domestic swine (sus scrofa).biting flies have been suggested as mechanical vectors of vesicular stomatitis new jersey virus (family rhabdoviridae, genus vesiculovirus, vsnjv) in livestock populations during epidemic outbreaks in the western united states. we conducted a proof-of-concept study to determine whether biting flies could mechanically transmit vsnjv to livestock by using a black fly, simulium vittatum zetterstedt (diptera: simuliidae), domestic swine, sus scrofa l., model. black flies mechanically transmitted vsn ...200919960709
sequential activation of ground pads reduces skin heating during radiofrequency ablation: initial in vivo porcine results.radiofrequency (rf) ablation is a common treatment modality for inoperable liver cancer. skin burns below ground pads during rf ablations are increasingly prevalent, hindering the development of higher-power rf generators capable of creating larger ablation zones.200919963820
african swine fever.african swine fever (asf) is a devastating haemorrhagic fever of pigs that causes up to 100% mortality, for which there is no vaccine. it is caused by a unique dna virus that is maintained in an ancient cycle between warthogs and argasid ticks, making it the only known dna arbovirus. asf has a high potential for transboundary spread, and has twice been transported from africa to other continents--europe and subsequently the caribbean and brazil (1957, 1959) and the caucasus (2007). it is also a ...200919967933
comparative animal study on hard tissue integration and bone formation of different nobel biocare implants.dental implantation aims at optimal and long-term hard tissue integration. beside primary stability, loading time and other factors, e.g. the surface of the endosteal part of the implant, is a matter of special importance. in this animal trial, hard tissue integration of two different implant types was studied using radiological, histological and histomorphometric analysis. two different implants with an oxidized surface (tiunite; nobel biocare ab, goteborg, sweden, nobelreplace tapered groovy 4 ...200920400804
brucella suis infection in domestic pigs and wild boar in croatia.between the years 2000 and 2004, 93,107 sera from 1,997 pig herds in 11 regions of croatia were tested for the presence of antibodies against brucellosis. positive results were observed in 67 herds from seven regions (mean individual prevalence: approximately 1%; herd prevalence: 3.4%). the herds from all but two of the infected farms were reared outdoors and thus almost certainly came into contact with wildlife. from 2003 to 2004, 424 sera, which were randomly collected from hunted wild boar (s ...200920462164
comparison of two different forms of bovine-derived hydroxyapatite in sinus augmentation and simultaneous implant placement: an experimental study.the aim of this study was to compare two different forms of bovine-derived hydroxyapatite (ha) in sinus augmentation and simultaneous implant placement.200919885412
concentrations of lipoproteins in the serum of wild boar and lithuanian pig breeds followed by principal component and cluster analyses to demonstrate the differences among genotypes.with the aim of evaluating serum cholesterol and triglyceride concentrations in lithuanian wild boar and lithuanian pig breeds, twelve gilts from three genotype groups were used in the experiment. only the concentration of high-density lipoprotein (hdl) was significantly higher in the serum of wild boar than in serum of domestic pig breeds (p < 0.05). factor loadings of the principal components (pcs) revealed that lipoproteins were positively related in pc1 for wild boars but associations betwee ...200919886266
the robust phylogeny of korean wild boar (sus scrofa coreanus) using partial d-loop sequence of mtdna.in order to elucidate the precise phylogenetic relationships of korean wild boar (sus scrofa coreanus), a partial mtdna d-loop region (1,274 bp, nc_000845 nucleotide positions 16576-1236) was sequenced among 56 korean wild boars. in total, 25 haplotypes were identified and classified into four distinct subgroups (k1 to k4) based on bayesian phylogenetic analysis using markov chain monte carlo methods. an extended analysis, adding 139 wild boars sampled worldwide, confirmed that korean wild boars ...200919855937
remarkable sequence similarity between the dinoflagellate-infecting marine girus and the terrestrial pathogen african swine fever virus.heterocapsa circularisquama dna virus (hcdnav; previously designated as hcv) is a giant virus (girus) with a approximately 356-kbp double-stranded dna (dsdna) genome. hcdnav lytically infects the bivalve-killing marine dinoflagellate h. circularisquama, and currently represents the sole dna virus isolated from dinoflagellates, one of the most abundant protists in marine ecosystems. its morphological features, genome type, and host range previously suggested that hcdnav might be a member of the f ...200919860921
tick-borne encephalitis from eating goat cheese in a mountain region of austria.we report transmission of tick-borne encephalitis virus (tbev) in july 2008 through nonpasteurized goat milk to 6 humans and 4 domestic pigs in an alpine pasture 1,500 m above sea level. this outbreak indicates the emergence of ticks and tbev at increasing altitudes in central europe and the efficiency of oral transmission of tbev.200919861072
characterization of selected nutrients and bacteria from anaerobic swine manure lagoons on sow, nursery, and finisher farms in the mid-south usa.swine (sus scrofa domestica) production in the mid-south usa comprises sow, nursery, and finisher farms. a 2007 packing plant closure started a regional shift from finisher to sow and nursery farms. changes in manure stored in lagoons and land-applied as fertilizer were expected but were unknown because nutrient and bacterial levels had not been characterized by farm type. the objectives of this study were to quantify selected nutrients and bacteria, compare levels by farm types, and project imp ...200919875798
new records of calyptrate dipterans (fanniidae, muscidae and sarcophagidae) associated with the decomposition of domestic pigs in brazil.the calyptrate dipterans are the most important decomposers of human cadavers. knowledge of their species and distribution are of great importance to forensic entomology, especially because of the enormous diversity in brazil. carcasses of domestic pigs (sus scrofa, l) were the experimental models used to attract calyptrates of forensic interest during the winters of 2006 and 2007 and the summers of 2006 and 2008. a total of 24,423 specimens from 44 species were collected (19 muscidae, 2 fanniid ...200919876568
elicitation of broad ctl response against hiv-1 by the dna vaccine encoding artificial multi-component fusion protein multihiv--study in domestic pigs.broad ctl response against hiv-1 is one factor that helps to control the viral replication. we have constructed a dna vaccine that encodes a large artificial fusion protein (multihiv) and shown it to be immunogenic in mice, swine and macaques. inbred mice revealed ctl response only against two epitopes due to limited mhc class i variability. to assess the quality of the ctl response we addressed this question in domestic swine. number of presented epitopes varied between 7 and 14 among the five ...200919879232
role of wild suids in the epidemiology of african swine fever.there is presently no vaccine to combat african swine fever (asf), a viral hemorrhagic fever of domestic pigs that causes up to 100% morbidity and mortality in naive, commercial pig populations. in its endemic setting, asf virus cycles between asymptomatic warthogs and soft ticks, with persistence in exotic locations being ascribed to the almost global distribution of susceptible soft tick and suid hosts. an understanding of the role played by diverse hosts in the epidemiology of this multi-host ...200919915917
animals to heal animals: ethnoveterinary practices in semiarid region, northeastern brazil.animal-based remedies constitute an integral part of traditional medicine and this is true in brazil as well both in rural and urban areas of the country. due to its long history, zootherapy has in fact become an integral part of folk medicine in the country. the use of these natural resources for medical purposes, however, is not restricted to human diseases treatment, being also widely used for the treatment of animal illnesses. ethnoveterinary is a science that involves the popular practical ...200919941663
[dynamics of heterozygosity and its correlation with fitness in a population of domestic pigs].variation of heterozygosity at 11 loci for blood group systems (erythrocyte antigens), occurred during 14 years in the domestic pig population of kemerovskaya breed is described. the two estimates computed for the population examined were represented by expected population heterozygosity, as a measure resistant to stochastic fluctuations, and individual heterozygosity, as a measure with the features of a quantitative trait. our results showed that relative fitness of genotypic classes, formed by ...200919947552
porcine cd18 mediates actinobacillus pleuropneumoniae apxiii species-specific toxicity.actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia, produces apx toxins that are recognized as major virulence factors. recently, we showed that apxiiia-cytotoxic activity specifically targets sus scrofa leukocytes. since both ltxa from aggregatibacter actinomycetem comitans (aggressive periodontitis in humans) and lkta from mannheimia haemolytica (pneumonia in ruminants) share this characteristic, respectively towards human and ruminant leukocytes, and because both ...200919356397
intra-abdominal pressure development after different temporary abdominal closure techniques in a porcine model.decompressive laparotomy followed by temporary abdominal closure (tac) is an established prophylaxis and treatment for abdominal compartment syndrome. the herein presented study aimed at the comparison of volume reserve capacity and development of intra-abdominal hypertension after forced primary abdominal closure and different tac techniques in a porcine model.200919359923
jejunal pouch reconstruction but not preservation of duodenal passage after total gastrectomy reduces plasma cholecystokinin and pancreatic polypeptide long term in pigs.the long-term effects of reconstructions of the gastrointestinal tract after gastrectomy on plasma levels of gastrointestinal hormones that contribute to food intake controls were evaluated.200919368131
total gastrectomy causes a sustained, long-term elevation of somatostatin in plasma, independent of the mode of reconstruction in pigs.the long-term effects of gastrectomy and various reconstructions of the gastrointestinal tract on fasting plasma levels of gastrointestinal hormones known to contribute to the control of gastrointestinal motor function were evaluated in pigs.200919368132
multipolar radiofrequency ablation in controlling hemorrhage from blunt liver trauma.the aim of this study is to investigate the use of multipolar radiofrequency (rf) ablation with internally cooled electrodes to control hemorrhage percutaneously from blunt liver trauma.200919371528
video-assisted lung resection using radiofrequency ablation in a porcine model.the aim of this study was to assess the feasibility, efficacy, and safety of video-assisted lingula resection using radiofrequency ablation (rfa) with internally cooled electrodes in a porcine model.200919376530
distribution of cocaine- and amphetamine-regulated transcript in the hippocampal formation of the guinea pig and domestic pig.this study provides a detailed description concerning the distribution of cocaineand amphetamine-regulated transcript (cart) subunits - cart(61-102) and rhcart(28-116) - in the hippocampal formation (hf) of the guinea pig and domestic pig, focussing on the dentate gyrus (dg) and hippocampus proper (hp). although in both studied species cart-immunoreactive (cart-ir) neuronal somata and processes were present generally in the same layers, some species-specific differences were still found. in the ...200919384826
[(18)f]fluoroacetate is not a functional analogue of [(11)c]acetate in normal physiology.[(11)c]acetate (c-ac) is a general pet tracer of cellular carbon flux and useful for clinical imaging in heart disease as well as prostate cancer and other tumours. c-ac has a high (70%) whole-body extraction fraction, proportional to blood flow in many organs. trapping is related to organ-specific enzymatic activation and formation of [(11)c]-acetyl-coa, the fate of which has been well characterized. due to the logistic challenges with c-ac, 2-[(18)f]fluoroacetate (f-ac) has been proposed as a ...200919387639
effect of recipient breed on delivery rate of cloned miniature pig.the miniature pig is regarded as a better organ donor breed for xenotransplantation than other pig breeds because the size of their organs is similar to that of humans. to improve efficiency of cloned miniature pig production, we analysed the effect of breed difference between donor cells and embryo recipients on pregnancy rate and delivery rate. cloned porcine embryos derived from domestic or miniature pig donor cells were transferred to domestic or miniature recipient pigs. delivery rate was s ...200919393119
occurrence and pathways of manure-borne 17beta-estradiol in vadose zone water.the hormone 17beta-estradiol (e2) can cause endocrine disruption in sensitive species at part per trillion concentrations. the persistence and transport pathways of manure-borne e2 in agricultural soils were determined by comparing its occurrence with the transfer of water and the transport of non-sorbing fluorobenzoic acid (fba) tracers. this comparison was done using capillary wick lysimeters installed 0.61m beneath three corn (zea mays l.) plots that receive swine (sus scrofa domesticus) manu ...200919394677
feral swine contact with domestic swine: a serologic survey and assessment of potential for disease transmission.feral swine (sus scrofa) are present in 38 of the 50 united states, and their populations continue to expand. domestic swine are widely regarded as vulnerable to diseases harbored by feral swine. our objectives were to determine antibody prevalence for selected pathogens in texas feral swine populations and identify contact events between feral and domestic swine. overall prevalence of antibodies against brucellosis and pseudorabies virus was 11% and 30%, respectively. antibodies to porcine repr ...200919395751
influence of sex on the meiotic segregation of a t(13;17) robertsonian translocation: a case study in the pig.comparison of male versus female meiotic segregation patterns for robertsonian translocation (rt) carriers with similar genetic background has rarely been reported in mammalian species.200919403564
porcine hypothalamic aromatase cytochrome p450: isoform characterization, sex-dependent activity, regional expression, and regulation by enzyme inhibition in neonatal boars.domestic pigs have three cyp19 genes encoding functional paralogues of the enzyme aromatase cytochrome p450 (p450arom) that are expressed in the gonads, placenta, and preimplantation blastocyst. all catalyze estrogen synthesis, but the gonadal-type enzyme is unique in also synthesizing a nonaromatizable biopotent testosterone metabolite, 1oh-testosterone (1oh-t). p450arom is expressed in the vertebrate brain, is higher in males than females, but has not been investigated in pigs, to our knowledg ...200919403926
pre-natal stress amplifies the immediate behavioural responses to acute pain in piglets.pre-natal stress (pns) or undernutrition can have numerous effects on an individual's biology throughout their lifetime. some of these effects may be adaptive by allowing individuals to tailor their phenotype to environmental conditions. here we investigated, in the domestic pig sus scrofa, whether one possible consequence of a predicted adverse environment could be altered pain perception. the behavioural response of piglets to the surgical amputation ('docking') of their tail or a sham procedu ...200919411272
relaxin and maternal lactocrine programming of neonatal uterine development.in mammals, including the pig (sus scrofa domesticus), structural patterning and functional programming of uterine tissues involve events that occur shortly after birth. porcine endometrial development between birth (postnatal day 0 [pnd 0]) and pnd 15 is estrogen receptor (er) dependent and estrogen sensitive. the endometrium is relaxin (rlx) receptor (rxfp1) positive and eralpha negative at birth. uterine expression of rxfp1 and eralpha, detectable by pnd 2, increases with age from pnd 0 to 14 ...200919416179
recombinant antigen targets for serodiagnosis of african swine fever.african swine fever (asf) is an infectious and economically important disease of domestic pigs. there is no vaccine, and so reliable diagnosis is essential for control strategies. the performance of four recombinant asf virus (asfv) protein (pk205r, pb602l, p104r, and p54)-based enzyme-linked immunosorbent assays (elisas) was evaluated with european porcine field sera that had been established by office international des epizooties (oie)-approved tests to be asfv negative (n = 119) and asfv posi ...200919420186
cadaveric volatile organic compounds released by decaying pig carcasses (sus domesticus l.) in different biotopes.forensic entomology uses pig carcasses to surrogate human decomposition and to investigate the entomofaunal colonization. insects communicate with their environment through the use of chemical mediators, which in the case of necrophagous insects, may consist in the cadaveric volatile organic compounds (vocs) released by the corpse under decomposition. previous studies have focused on cadaveric vocs released from human corpses. nevertheless, studies on human corpses are restricted for many reason ...200919423246
pure 'natural orifice transluminal endoscopic surgery' for transvaginal nephrectomy in the porcine model.to determine the technical feasibility and reproducibility of pure natural orifice transluminal endoscopic surgery (notes) transvaginal nephrectomy using notes-specific instrumentation, with no transabdominal assistance.200919426194
an integrated rh map of porcine chromosome 10.whole genome radiation hybrid (wg-rh) maps serve as "scaffolds" to significantly improve the orientation of small bacterial artificial chromosome (bac) contigs, order genes within the contigs and assist assembly of a sequence-ready map for virtually any species. here, we report the construction of a porcine: human comparative map for pig (sus scrofa) chromosome 10 (ssc10) using the imnprh2(12,000-rad) porcine wg-rh panel, integrated with the imprh(7000-rad) wg-rh, genetic and bac fingerprinted c ...200919426492
detection of hepatitis e virus in wild boars of rural and urban regions in germany and whole genome characterization of an endemic strain.hepatitis e is an increasingly diagnosed human disease in central europe. besides domestic pigs, in which hepatitis e virus (hev) infection is highly prevalent, wild boars have been identified as a possible source of human infection. in order to assess the distribution of hev in the wild boar population of germany, we tested liver samples originating from different geographical regions for the presence of the hev genome and compared the detected sequences to animal and human hev strains.200919442307
endemic hepatitis e in two nordic countries.antibodies against hepatitis e virus (anti-hev) were found in 248 swedish and danish patients between 1993 and 2007. most patients were symptomatic and tested for anti-hev due to travel abroad. among patients with known country of infection, most were infected in asia, mainly on the indian subcontinent. however, 29 patients were infected in europe, nine of these had hev igm and/or hev rna in serum. in sera from 65 of 141 tested patients hev rna could be detected, and 63 strains could be typed by ...200919442399
recording diffusion responses from contralateral intercostal muscles after stimulus-triggered electromyography: refining a tool for the assessment of thoracic pedicle screw placement in an experimental porcine model.a new stimulus-triggered electromyography (emg) test for detecting stimulus diffusion to contralateral intercostal muscles during thoracic pedicle screw placement was assessed in a porcine model.200919444052
comparison of vascularized osteoperiosteal femur flaps and nonvascularized femur grafts for reconstruction of mandibular defects: an experimental study.the aim of this study was to compare the vascularized osteoperiosteal femur flaps (vffs) and the nonvascularized femur grafts (nvfgs) for reconstruction of the mandibular defects of pigs.200919446201
wound healing and the immune response in swine treated with a hemostatic bandage composed of salmon thrombin and fibrinogen.we investigated the inflammatory response in pigs exposed to salmon fibrinogen/thrombin dressings. animals were exposed to the material in 3 ways: (a) thrombin and fibrinogen were injected intravenously, (b) dual full-thickness skin lesions were surgically created on the dorsal aspect of the swine and treated with the fibrinogen/thrombin bandage and a commercial bandage or (c) a fibrinogen/thrombin bandage was inserted through an abdominal incision into the peritoneal cavity. blood was collected ...200919449202
prevention of vasa vasorum neovascularization attenuates early neointima formation in experimental hypercholesterolemia.vasa vasorum (vv) neovascularization is a key feature of early atherosclerosis and adds substantial endothelial exchange-surface to the coronary vessel wall. thus, it is conceivable that vv neovascularization favors the entry of pro-inflammatory and pro-atherosclerotic blood components into the coronary vessel wall. we sought to investigate the effects of thalidomide (th), a potent anti-angiogenic drug on vasa vasorum (vv) neovascularization, vessel wall inflammation, and neointima formation in ...200919458984
molecular taxonomy, phylogeny and biogeography of nematodes belonging to the trichinella genus.studying parasites of the genus trichinella provides scientists of today many advantages. this is a group of zoonotic nematodes that circulates freely among wildlife hosts with one in particular, trichinella spiralis that is exceptionally well adapted to domestic swine. recent reports suggest that human infections from hunted animals are on the rise worldwide and numerous countries still experience problems with t. spiralis in their domestic food supplies. trichinella is a genus whose members ar ...200919460327
meat juice as diagnostic sample for virological and serological diagnosis of classical swine fever.the objective of this paper was to assess if meat juice is a suitable substrate for virological and serological diagnosis of classical swine fever (csf). fifty-six domestic pigs and 21 wild boars experimentally vaccinated and/or infected as well as 129 field samples from wild boars were involved in this study. meat juice from diaphragm, forequarter and hindquarter was used for investigations. csfv and viral rna were detected in meat juice between days 5 and 21 post infection (pi). animals which ...200919462640
imaging of the midpalatal suture in a porcine model: flat-panel volume computed tomography compared with multislice computed tomography.an investigation was conducted to compare the image quality of prototype flat-panel volume computed tomography (fpvct) and multislice computed tomography (msct) of suture structures.200919464211
comparative analysis of solar radiation-induced cellular damage between ex vivo porcine skin organ culture and in vitro reconstructed human epidermis.reconstructed human epidermis models (rhe) constitute an innovative alternative to study phototoxicity and photoprotection in the cosmetic industry. however, little information is currently available concerning the harmful effects of solar-simulated radiation (ssr) in these in vitro skin models. in this study, the phototoxic effects of a single acute ssr dose of 275 kj m(-2) were evaluated in a validated rhe model (from skinethic), and were compared with those obtained from an ex vivo skin organ ...200919467030
trichuris suis and trichuris trichiura are different nematode species.in this paper, a morphological and biometrical study by optical microscopy and scanning electronic microscopy (sem) of trichuris suis isolated from different hosts (sus scrofa domestica and sus scrofa scrofa) and trichuris trichiura isolated from chimpanzee, has been carried out. our results demonstrate the existence of typical pericloacal papillae in both species. biometrical parameters of t. suis and t. trichiura overlapped but males and females of t. trichiura tended to be shorter and thinner ...200919467214
usefulness of mri to differentiate between temporary and long-term coronary artery occlusion in a minimally invasive model of experimental myocardial infarction.the surgical technique employed to determine an experimental ischemic damage is a major factor in the subsequent process of myocardial scar development. we set out to establish a minimally invasive porcine model of myocardial infarction using cardiac contrast-enhanced magnetic resonance imaging (ce-mri) as the basic diagnostic tool. twenty-seven domestic pigs were randomized to either temporary or permanent occlusion of the left anterior descending artery (lad). temporary occlusion was achieved ...200919472001
receptor activator of nuclear factor kappa b ligand in an experimental intervertebral disc degeneration.this study was designed to clarify the role of the receptor activator of nuclear factor kappa b ligand (rankl) in the process of discus degeneration and spondylarthrosis. it was hypothesized that experimental discus lesion would initiate not only local bone remodelling but also increased osteoclast formation on a location remote to the injury site due to altered spinal biomechanics. it was speculated that these changes in vertebral bone remodelling could be reflected in an increased rankl expres ...200919473572
isolation and fluorescence spectrum of cardiac myosin from pig heart.cardiac myosin is the most abundant and bioactive protein presented in heart tissue. in this work, a simple method was described for preparation of high purity cardiac myosin for further research. with the use of a domestic pig left ventricle as the starting tissue, this method yielded 4.5 mg myosin per gram of wet tissue with a specific activity of 0.293 u/mg. the purification factor was 4.9-fold with an activity recovery of 42.1%. in addition, the investigation of fluorescence spectrum shown t ...200919475499
percutaneous left ventricular assist in ischemic cardiac arrest.ischemic cardiac arrest represents a challenge for optimal emergency revascularization therapy. a percutaneous left ventricular assist device (lvad) may be beneficial.200919242324
epinephrine reduces cerebral perfusion during cardiopulmonary resuscitation.epinephrine has been the primary drug for cardiopulmonary resuscitation (cpr) for more than a century. the therapeutic rationale was to restore threshold levels of myocardial and cerebral blood flows by its alpha1 (alpha1) and alpha2 (alpha2)-adrenergic agonist vasopressor actions. on the basis of coincidental observations on changes in microvascular flow in the cerebral cortex, we hypothesized that epinephrine selectively decreases microvascular flow.200919242339
phase-contrast mri-based elastography technique detects early hypertensive changes in ex vivo porcine aortic wall.to measure the elastic properties of ex vivo porcine aortas in control and hypertensive groups using a phase contrast magnetic resonance imaging (mri)-based elastography technique.200919243040
a stochastic model to quantify the risk of introduction of classical swine fever virus through import of domestic and wild boars.classical swine fever (csf) is a disease of pigs that imposes major hardship on the industry of infected regions. the recent history of csf epidemics suggests that animal movements remain the main source of csf virus (csfv) infection for susceptible populations in europe. this study presents an assessment of the risk of introducing csfv into spain through the importation of live susceptible animals. results suggest that, if prevailing conditions persist, introduction of csfv into spain is likely ...200919243649
isolate designation and characterization of toxoplasma gondii isolates from pigs in the united states.pigs are considered to be the most important meat source of toxoplasma gondii for humans in the united states. in the present study, 168 t. gondii isolates (designated tgpgus15-182) from various sources were genotyped using 10 polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) markers (sag1, sag2, sag3, btub, gra6, c22-8, c29-2, l358, pk1, and apico). genotyping data from an additional 14 isolates collected from t. gondii-infected pigs in maryland were included for ana ...200919245283
the effects of fasting and general anesthesia on serum chemistries in kcg miniature pigs.investigators are obligated to optimize the perioperative care of experimental animals, but little is known about the effects of anesthesia and surgery on serum chemistries in kcg pigs. the objective of this study was to examine the influence of fasting and surgery under general anesthesia on 27 serum chemistries in kcg miniature pigs to improve management. crossbred kcg minipigs were used at a mean of 12.3 mo of age (range, 8.6 to 14.9) and 33.4 kg of body weight (range, 24.0 to 40.2). serum ch ...200919245748
effects of streptozotocin-induced diabetes in domestic pigs with focus on the amino acid metabolism.streptozotocin (stz) given intravenously destroys pancreatic beta cells and is widely used in animal models to mimic type 1 diabetes. the effects of stz on the clinical state of health and metabolism were studied in six high health certified domestic pigs weighing 19+/-1.3 kg at the start of the experiment. a single stz dose of 150 mg/kg of body weight successfully induced hyperglycaemia and alterations in amino acid metabolism. within 9 h after stz administration, the blood glucose values fell ...200919246503
animal evolution during domestication: the domesticated fox as a model.we review the evolution of domestic animals, emphasizing the effect of the earliest steps of domestication on its course. using the first domesticated species, the dog (canis familiaris), for illustration, we describe the evolutionary peculiarities during the historical domestication, such as the high level and wide range of diversity. we suggest that the process of earliest domestication via unconscious and later conscious selection of human-defined behavioral traits may accelerate phenotypic v ...200919260016
cysticercosis of soft tissue.cysticercosis is a systemic illness caused by dissemination of the larval form of the pork tapeworm, taenia solium. a high prevalence has been reported from the developing countries because of the co-existence of poor sanitary conditions and domestic pig raising. human cysticercosis occurs when eggs are ingested via faecal-oral transmission from a tapeworm host. the human then becomes an accidental intermediate host, with development of cysticerci within organs. cysticercosis is a preventable fa ...200919260577
the association between central venous pressure, pneumoperitoneum, and venous carbon dioxide embolism in laparoscopic hepatectomy.laparoscopic hepatectomy (lh) is increasingly used. however, the safety and outcomes of lh have yet to be elucidated. the risk of venous gas embolism is increased during liver parenchymal transection. this risk may be increased with positive pressure carbon dioxide (co(2)) pneumoperitoneum (pp). this may be exacerbated further when low central venous pressure (cvp) anesthesia is used to minimize hemorrhage during liver resection.200919266234
giardia genotypes in pigs in western australia: prevalence and association with diarrhea.little is known of the prevalence of giardia species and genotypes in pre- and post-weaned domestic pigs. in the present study, a total of 297 pig faecal samples were screened for the presence of giardia by pcr and genotyped. an overall prevalence of 31.1% (90/289) (25.8, 36.5 ci) was detected. giardia was detected in 17% (23/123) (11.8-25.6 ci) of pre-weaned piglet faecal samples and 41% (64/156) (33.3-48.7 ci) post-weaned faecal samples analysed. sequence analysis identified assemblage a and e ...200919271283
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