Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| a phase ii clinical trial of mefloquine in brazilian male subjects. | mefloquine was compared with sulfadoxine-pyrimethamine for safety and efficacy in a randomized, double-blind clinical trial in adult males from a malaria-endemic area of brazil. a total of 99 oligosymptomatic and symptomatic volunteers with plasmodium falciparum parasitaemia took part in the trial; 49 were given 1000 mg of mefloquine and the remainder received 1500 mg of sulfadoxine plus 75 mg of pyrimethamine, in a single oral dose.mefloquine was 100% successful in clearing parasitaemia within ... | 1983 | 6360401 |
| epidemiology of seasonal falciparum malaria in an urban area of senegal. | a 15-month longitudinal survey was carried out to examine entomological and parasitological aspects of human malaria transmission in pikine, a city located in the sudan savanna zone on the cap vert peninsula in the west of senegal. the anopheline population was sampled twice weekly indoors by night human bait capture. during the same period, thick and thin blood films were collected from 296 children at 2-month intervals. anopheles arabiensis was the only species responsible for transmission of ... | 1983 | 6360402 |
| preliminary report: iron metabolic changes in plasmodium falciparum malaria. | 1983 | 6360652 | |
| abo blood groups and infections with human malarial parasites in vivo and in vitro. | 1983 | 6360653 | |
| in vitro culture as a source of plasmodium falciparum antigen in micro-elisa in malaria. | 1983 | 6360860 | |
| the role of cell-mediated immune responses in resistance to malaria, with special reference to oxidant stress. | asexual blood forms of malaria parasites are microaerophilic and sensitive to oxidant stress. plasmodium falciparum and some other species of malaria parasites undergo schizogony attached to endothelial cells of postcapillary venules, where oxygen tensions are low. acquired immune responses to all forms of malaria parasites so far investigated are thymus dependent. animals deprived of t lymphocytes do not recover from the infections and cannot be immunized against malaria parasites. in contrast, ... | 1983 | 6100538 |
| polyamine oxidase-mediated intraerythrocytic killing of plasmodium falciparum: evidence against the role of reactive oxygen metabolites. | the polyamines spermine and spermidine, in the presence of polyamine oxidase, were shown to be cytotoxic in vitro to various isolates of plasmodium falciparum. neither polyamines nor polyamine oxidase alone was cytotoxic. this cytotoxicity was manifested by the degeneration of the parasites into crisis forms and by the inhibition of methionine incorporation by the parasites. only 2 to 2.5 h of exposure to the reaction mixture (polyamine oxidase, 100 micrograms/ml; spermine, 1 mm) resulted in par ... | 1984 | 6360897 |
| plasmodium falciparum: protein antigens identified by analysis of serum samples from vaccinated aotus monkeys. | serum samples from aotus trivirgatus subsp. griseimembra monkeys obtained at different stages of a vaccination experiment were analyzed for total antibody titer to plasmodium falciparum and were used for identifying protective antigens of the human malaria parasite. total malarial antibody titers were higher in serum samples from protected monkeys (vaccinated with antigen in an adjuvant) than in those from unprotected monkeys (vaccinated with either antigen or adjuvant only). parasite proteins w ... | 1984 | 6360901 |
| serotyping plasmodium falciparum from acute human infections using monoclonal antibodies. | monoclonal antibodies specific for the schizont and merozoite stages of plasmodium falciparum have been used to demonstrate antigenic differences between parasites obtained from individual patients with acute malaria. parasites from east africa are shown to share some strain-specific antigenic determinants with culture-adapted isolates from africa as well as from asia and papua new guinea. | 1984 | 6200967 |
| antigenic diversity found in isolates of plasmodium falciparum from papua new guinea by using monoclonal antibodies. | monoclonal antibodies were used to demonstrate antigenic diversity in over 100 primary isolates of plasmodium falciparum collected from one area of papua new guinea. the frequencies of several parasite antigens in our sample is calculated. one particular antigen which had previously been shown to be absent in one-third of established isolates collected from several countries appeared ubiquitous in our sample from papua new guinea. a method is presented that can be used to test for associations b ... | 1984 | 6201077 |
| evidence for immunological cross-reaction between sporozoites and blood stages of a human malaria parasite. | malaria parasites (plasmodium spp.) show a complex pattern of development in the mammalian host and many studies support the view that the surface of the sporozoite, injected by the mosquito, has no antigens in common with the erythrocytic stage of development. for example, immunization with the erythrocytic parasites generates antisera with negligible titre by indirect immunofluorescence to the sporozoite surface. although monoclonal antibodies prepared against erythrocytic stages were reported ... | 1984 | 6199678 |
| a high molecular weight antigen in plasmodium falciparum recognized by inhibitory monoclonal antibodies. | inhibitory monoclonal antibodies which bind to some isolates of plasmodium falciparum from papua new guinea, but not from other areas, bound to a 220 kd antigen. by immunofluorescence microscopy this antigen was shown to be located both within the schizont cytoplasm and also within the schizont infected erythrocyte, but external to the schizont itself. even at antibody concentrations which caused greater than 70% inhibition of parasite multiplication, accumulation of schizont stages or aggregate ... | 1984 | 6199717 |
| cloning of malarial genes coding for high molecular weight antigens: isolation of fragments of plasmodium falciparum genes coding for proteins of 145 000 molecular weight. | rna preparations from plasmodium falciparum were shown to direct the cell free synthesis of many high molecular weight proteins, some of which could be immunoprecipitated by sera from humans immune to p. falciparum. among these was an abundant protein with a molecular weight of 145 000. messenger rnas coding for high molecular weight antigens were enriched by size fractionation and used after reverse transcription to identify genomic restriction fragments in southern blots. genomic restriction f ... | 1984 | 6198588 |
| cross reactions between plasmodium falciparum and mammalian tissue antigens detected by monoclonal antibodies. | 1984 | 6202251 | |
| characterisation and translation studies of messenger rna from the human malaria parasite plasmodium falciparum and construction of a cdna library. | rna was isolated from trophozoites, schizonts and mixed populations of plasmodium falciparum. 5% of the total was poly(a+) message, of average length 1.2 kb (10-12 kb maximum) and a poly(a) content of 10%. the mrna fractions could be translated in vitro by reticulocyte lysates supplemented either with exogenous or p. falciparum trna. the patterns from two independent isolates, one cloned (t9-94) and one uncloned (k1) were virtually identical. major translation products from 16-230 kda have been ... | 1984 | 6203036 |
| species- and stage-specific antigens in exoerythrocytic stages of plasmodium falciparum. | numerous exoerythrocytic forms of plasmodium falciparum ( pfeef ) were obtained from the liver of the south american monkey, cebus apella, for analysis of the antigens on this stage. as antigen for the fluorescent assay, 5-micron sections of liver fragments collected on day 5 following sporozoite inoculation and fixed in carnoy's solution or kept in liquid nitrogen were used. two types of fluorescent labeling of the pfeef were identified: diffuse and peripheral. each of 23 sera from individuals ... | 1984 | 6203418 |
| structure of the gene encoding the immunodominant surface antigen on the sporozoite of the human malaria parasite plasmodium falciparum. | the gene for the circumsporozoite (cs) protein of plasmodium falciparum has been cloned and its nucleotide sequence determined. the gene encodes a protein of 412 amino acids as deduced from the nucleotide sequence. the protein contains 41 tandem repeats of a tetrapeptide, 37 of which are asn-ala-asn-pro and four of which are asn-val-asp-pro. monoclonal antibodies against the cs protein of plasmodium falciparum were inhibited from binding to the protein by synthetic peptides of the repeat sequenc ... | 1984 | 6204383 |
| dna cloning of plasmodium falciparum circumsporozoite gene: amino acid sequence of repetitive epitope. | a clone of complementary dna encoding the circumsporozoite (cs) protein of the human malaria parasite plasmodium falciparum has been isolated by screening an escherichia coli complementary dna library with a monoclonal antibody to the cs protein. the dna sequence of the complementary dna insert encodes a four-amino acid sequence: proline-asparagine-alanine-asparagine, tandemly repeated 23 times. the cs beta-lactamase fusion protein specifically binds monoclonal antibodies to the cs protein and i ... | 1984 | 6204384 |
| processing, polymorphism, and biological significance of p190, a major surface antigen of the erythrocytic forms of plasmodium falciparum. | a detailed analysis of p190, a major surface associated protein of plasmodium falciparum erythrocytic stages has been undertaken. we have demonstrated that this protein is recognised by two monoclonal antibodies, one of which recognises a constant feature (2.2) and one a variable feature (7.3). cell free protein synthesis demonstrates that the variable 7.3 epitope is encoded in the structural gene for p190. the 7.3 epitope is only present on late trophozoites and schizonts whilst the 2.2 epitope ... | 1984 | 6205267 |
| histidine-rich protein genes and their transcripts in plasmodium falciparum and p. lophurae. | the presence of histidine-rich protein (hrp) related genes and gene products in plasmodium falciparum was demonstrated using a synthetic pentahistidine-encoding oligonucleotide and a cloned hrp cdna probe prepared from the avian parasite p. lophurae. in northern blotting experiments, two knobby clones of p. falciparum were found to contain a 3500 nucleotide rna species that hybridized with the oligonucleotide and hrp cdna probes. as this component had the expected size for an mrna encoding an 80 ... | 1984 | 6205271 |
| surface proteins of plasmodium falciparum merozoites binding to the erythrocyte receptor, glycophorin. | invasion of erythrocytes by the malarial parasite is a receptor-mediated process. p. falciparum merozoites recognize and bind to erythrocyte surface sialoglycoproteins, glycophorins a and b, and the glycophorins bind to saturable sites on the merozoite surface. the purpose of the present work was to identify a receptor or ligand molecule on the merozoite surface that mediates binding to the erythrocyte. a fraction containing the sialoglycoproteins was coupled to an acrylamide matrix and incubate ... | 1984 | 6206188 |
| evolutionary conservation of histidine-rich protein genes and rnas in malaria parasites. | 1984 | 6206515 | |
| evaluation of a range of antimicrobial agents against the parasitic protozoa, plasmodium falciparum, babesia rodhaini and theileria parva in vitro. | eighteen antimicrobials commonly used in tissue culture were screened in three different protozoan test systems in order to establish their suitability for routine inclusion in protozoal cultivation systems. the human malaria parasite, plasmodium falciparum, was inhibited by more than half the antibiotics tested at concentrations recommended for normal tissue culture use. eight compounds were well tolerated and thus could be used prophylactically to prevent microbial contamination. these antimic ... | 1984 | 6206808 |
| an antigenic determinant which is variant in a population of plasmodium falciparum is present in isolates of plasmodium malariae. | a monoclonal antibody that is directed against an antigenic determinant which is variant in a population of plasmodium falciparum also reacts with plasmodium malariae. | 1984 | 6207738 |
| knob-positive and knob-negative plasmodium falciparum differ in expression of a strain-specific malarial antigen on the surface of infected erythrocytes. | we have investigated the expression of a strain-specific malarial antigen on the surface of erythrocytes infected with knobless (k-) variants of knob-positive (k+) strains of plasmodium falciparum. aotus blood infected with k+ or k- parasites derived from two independent geographical isolates (malayan camp and santa lucia) was surface iodinated by the lactoperoxidase method. infected and uninfected erythrocytes were then separated by a new procedure involving equilibrium density sedimentation on ... | 1984 | 6208311 |
| regulation of the immune response in plasmodium falciparum malaria. iii. proliferative response to antigen in vitro and subset composition of t cells from patients with acute infection or from immune donors. | t cells from patients with acute plasmodium falciparum malaria were investigated for their proliferative responses in vitro to malarial antigen. of 26 patients, 14 had acute and short lived (less than or equal to 8 days) disease episodes, most of them for the first time, while 12 had been ill for more than 8 days at the time of the blood samples were taken. the lymphocytes from the first group gave a weak, and apparently p. falciparum specific proliferation, peaking after 3-4 days, but waning wi ... | 1984 | 6209042 |
| source of serum ferritin in malaria. | 1984 | 6210046 | |
| resistance of plasmodium falciparum. | 1984 | 6210209 | |
| [an additional argument in favor of the protective effect of glucose 6-phosphate dehydrogenase deficiency against malaria?]. | 1984 | 6229750 | |
| prolonged incubation improves the micro-scale in-vitro test for drug sensitivity of plasmodium falciparum. | in the standard micro-scale in-vitro test for detecting resistance of plasmodium falciparum to chloroquine, isolates are incubated for 24-28 h in medium that does not contain serum. the effect of prolonging incubation to 48 h and of adding human ab serum to the culture medium of p falciparum from irian jaya, indonesia was studied. addition of serum was not associated with any advantages or disadvantages. production of schizonts was increased when incubation was prolonged. this facilitated readin ... | 1984 | 6140392 |
| failure of chloroquine-erythromycin and chloroquine-tetracycline combinations in treatment of chloroquine-resistant falciparum malaria in eastern thailand. | in eastern thailand the increasing resistance of plasmodium falciparum to all available antimalarial drugs prompted a reassessment of chloroquine in combination with either erythromycin or tetracycline. both combinations had produced encouraging results in vitro, in experimental animal models, or in preliminary clinical trials. in patients with uncomplicated falciparum malaria presenting with moderate parasitaemia the trial of chloroquine and tetracycline was abandoned after riii resistance with ... | 1984 | 6141378 |
| effectiveness of amodiaquine as treatment for chloroquine-resistant plasmodium falciparum infections in kenya. | studies were conducted in malindi, kenya, to assess the response of plasmodium falciparum to chloroquine and amodiaquine in vivo (by an extended 14-day test) and in vitro (with the rieckmann micro test). in-vivo resistance was demonstrated in 19 of 69 (28%) infections treated with chloroquine, but in only 2 of 60 (3.3%) of those treated with amodiaquine (p less than 0.001). in-vitro resistance to chloroquine was demonstrated in 15 of 23 (65%) tests. in contrast, 22 of the same 23 isolates were s ... | 1984 | 6141423 |
| human lymphocytes transformed with epstein-barr virus produce antibodies against sporozoite stage of plasmodium falciparum. | 1984 | 6142241 | |
| analysis of clinical specimens by hybridisation with probe containing repetitive dna from plasmodium falciparum. a novel approach to malaria diagnosis. | the capacity of a dna probe containing cloned repetitive sequences from plasmodium falciparum to identify malaria-infected blood samples was tested with a spot hybridisation assay. parasitaemia levels of 0.001% could be detected in 50 microliters blood from patients. the probe correctly diagnosed p falciparum infection in patients from different continents and appeared to be specific for p falciparum, since it did not cross-react with three other plasmodium species tested. | 1984 | 6142251 |
| dna probes for malaria diagnosis. | 1984 | 6143111 | |
| glycophorin c and the invasion of red cells by plasmodium falciparum. | 1984 | 6143210 | |
| amodiaquine more effective than chloroquine against plasmodium falciparum malaria on kenya coast. | 1984 | 6143883 | |
| blood levels and in vitro activity of desethylchloroquine against plasmodium falciparum. | 1984 | 6144914 | |
| chloroquine-resistant malaria from angola. | 1984 | 6144960 | |
| a commuter mosquito's second bite? | 1984 | 6145015 | |
| amodiaquine and chloroquine efficacy against plasmodium falciparum in madagascar. | 1984 | 6145017 | |
| identification of circumsporozoite proteins in individual malaria-infected mosquitoes by western blot analysis. | circumsporozoite (cs) proteins of rodent (plasmodium berghei), simian (p. knowlesi), and human (p. falciparum) malaria parasites extracted from dead and dried mosquitoes have been identified by the western blot (immunoblot) technique. dried mosquitoes which were laboratory-reared and infected with plasmodium or freshly dissected sporozoites were triturated in sample reducing buffer and the extracts electrophoresed in a 10% sds-polyacrylamide gel. after transferring the proteins to nitrocellulose ... | 1984 | 6145742 |
| absence of alpha-ketoglutarate dehydrogenase activity and presence of co2-fixing activity in plasmodium falciparum grown in vitro in human erythrocytes. | plasmodium falciparum-infected human erythrocytes grown in vitro do not release 14co2 when incubated in the presence of [1-14c]glutamate, despite the presence of glutamate dehydrogenase, implying the absence of alpha-ketoglutarate dehydrogenase activity and the lack of functional tricarboxylic acid cycle in the human malaria parasite. cultures incubated with [14c]bicarbonate, however, fix co2 into acid-stable metabolites; co2 fixation proceeds linearly for up to two hours after an initial brief ... | 1984 | 6145796 |
| chloroquine-resistant plasmodium falciparum malaria from angola. | 1984 | 6145893 | |
| chloroquine resistant plasmodium falciparum malaria in namibia. | 1984 | 6145958 | |
| in vitro translation of plasmodium falciparum proteins. | rna was prepared from cultured plasmodium falciparum using ribonucleoside-vanadyl ribonuclease inhibitors and assayed in a rabbit reticulocyte, cell-free lysate translation system. discrete proteins, ranging from less than 10,000 to greater than 200,000 daltons, were synthesized. some of these proteins were precipitated by immune serum and were similar to those metabolically synthesized. purified poly [a] mrna was also translated and differences between the fractions which either bind or pass th ... | 1984 | 6147129 |
| wr(a+b-)red blood cells are fully susceptible to invasion by plasmodium falciparum. | 1984 | 6147533 | |
| wrb antigen not required for invasion of human erythrocytes by plasmodium falciparum. | 1984 | 6147534 | |
| chemoprophylaxis of malaria in non-immune residents in dar es salaam, tanzania. | the malaria infection rates in non-immune residents of dar es salaam on various chemoprophylactic regimens were compared with that (37.1%) in those not taking prophylactic antimalarials. among 647 people resident in dar es salaam for 1-6 years the two groups with the lowest infection rates by person-episodes (2.0% and 1.5%) were those taking proguanil 200 mg daily alone or with chloroquine base 300 mg weekly. infection rates (16.9% and 14.0%) were also significantly lower than in the no-prophyla ... | 1984 | 6147692 |
| in-vitro culture of exoerythrocytic form of plasmodium falciparum in adult human hepatocytes. | 1984 | 6148510 | |
| wrb negative erythrocytes are susceptible to invasion by malaria parasites. | 1984 | 6148511 | |
| wright's b determinant, monoclonal antibodies, and plasmodium falciparum merozoite invasion. | 1984 | 6148655 | |
| [current world malariology situation and the problem of the importation of malaria into the ussr]. | 1984 | 6148689 | |
| chloroquine-resistant malaria in pakistan. | 1984 | 6149382 | |
| pyrimidine de novo synthesis during the life cycle of the intraerythrocytic stage of plasmodium falciparum. | the 6 enzymes involved in de novo synthesis of pyrimidines were measured in plasmodium falciparum isolated by saponin lysis from rbc's nonsynchronized and synchronized in vitro cultures. the total activities were found to be dependent on the stage of the p. falciparum cycle. in parasites isolated from synchronized cultures, the highest activities for all enzymes were found at about 27 hr after synchronization in the late trophozoite stage, or just before schizont formation. merozoites and ring f ... | 1984 | 6150076 |
| chloroquine-resistant malaria in india. | 1984 | 6150218 | |
| malaria control in tanzania. | 1984 | 6150219 | |
| randomised comparative study of mefloquine, qinghaosu, and pyrimethamine-sulfadoxine in patients with falciparum malaria. | a prospective trial in 80 patients randomly allocated to four antimalarial treatment regimens--mefloquine plus pyrimethamine-sulfadoxine ('fansidar'); mefloquine plus qinghaosu; mefloquine, fansidar, and qinghaosu; and qinghaosu alone--was carried out on hainan island, china, in patients with chloroquine-resistant falciparum malaria. a radical cure with slight side-effects was obtained with mefloquine plus fansidar; the addition of qinghaosu greatly increased the rate of parasite clearance with ... | 1984 | 6150365 |
| malaria prophylaxis in travellers: the current position. | malaria prevention is a main challenge for physicians, nurses, health officers and tour operators. the attack rate of malaria in travellers is 1-10/10,000 departures, and the case fatality rate of imported malaria is around 0.5/100. travellers should be informed about the risk they are going to take, how to protect against mosquito bites, about the antimalarials they will have to take and what to do when a malaria breakthrough should occur. the 4-aminoquinolines (chloroquine, amodiaquine) remain ... | 1984 | 6150865 |
| plasmodium falciparum transmission in england: entomological and epidemiological data relative to cases in 1983. | 1984 | 6151004 | |
| antigenic variation of bloodstage malaria parasites. | an antigen on the surface of erythrocytes infected with mature asexual malaria parasites has been shown to undergo antigenic variation in two malaria species. plasmodium falciparum-infected erythrocytes from squirrel monkeys express a new antigen that is identified by reactivity with antibody from infected animals in an indirect immunofluorescence assay. cloned p. knowlesi parasites in rhesus monkeys undergo antigenic variation of an erythrocyte surface antigen as defined by antibody-mediated ce ... | 1984 | 6151679 |
| protective antigens of rodent and human bloodstage malaria. | bloodstage malaria parasites are antigenically complex, but individual antigens can be identified and analysed using monoclonal antibodies. two monoclonal antibodies that recognize a 235 000 molecular mass plasmodium yoelii rhoptry protein provide some protection when injected into mice against a challenge infection. the purified rhoptry protein also provides protective immunity against p. yoelii ym when used to vaccinate mice and fulminating infections are converted into self-limiting, reticulo ... | 1984 | 6151681 |
| the expression of plasmodium falciparum bloodstage antigens in escherichia coli. | a library of cdna clones expressing proteins of the asexual blood stages of a papua new guinean isolate of plasmodium falciparum (isolate fcq27/png (fc27] was constructed in the bacteriophage vector lambda gt11-amp3. in an in situ colony immunoassay, human serum was used to identify colonies producing natural immunogens. sera from donors of defined clinical status, or reactive to a defined subset of natural immunogens were used to identify clones of particular interest (for example, clones react ... | 1984 | 6151682 |
| receptors on red cells for plasmodium falciparum and their interaction with merozoites. | the red cell sialoglycoproteins (glycophorins, alpha (a), delta (b) and beta and gamma (c] play a crucial role in the invasion of human red cells by merozoites of plasmodium falciparum. red cells deficient in any of the glycophorins, including beta (also known as glycoconnectin), resist infection by this parasite to varying degrees. these cells and other naturally occurring well-characterized glycophorin variants provide extremely powerful tools to dissect the role of these molecules in invasion ... | 1984 | 6151683 |
| the ring-infected erythrocyte surface antigen (resa) polypeptide of plasmodium falciparum contains two separate blocks of tandem repeats encoding antigenic epitopes that are naturally immunogenic in man. | we showed previously that the ring-infected erythrocyte surface antigen (resa) of plasmodium falciparum contains a repetitive amino acid sequence. we have investigated here the sequence and antigenic relationships between resa from fc27, a papua new guinea isolate, and from nf7, a ghanaian isolate. the complete nucleotide sequences of eight different cdna clones demonstrate that resa from the two strains are closely homologous over the region that can be compared. a series of related eight, four ... | 1984 | 6085696 |
| type-i htlv antibody in urban and rural ghana, west africa. | the prevalence of antibodies against the newly described human t-cell lymphoma virus, type i (htlv-i) in two communities in ghana, west africa, is described. there was no difference by community (urban, 3.6% and rural, 4.0%). prevalence increased with age, being 5.9% among persons greater than 10 years old, but did not differ by sex. there was no difference when data were analysed by housing status or crowding. non-confirmed reactions in the assay system were frequent and correlated with both pr ... | 1984 | 6088402 |
| major surface antigen gene of a human malaria parasite cloned and expressed in bacteria. | the late blood stages of the human malaria parasite, plasmodium falciparum, carry a major surface antigen, p190, of molecular weight (mr) 190,000. this antigenically variable protein is actively processed, first as the parasite matures and again when it is released into the blood stream and invades a new erythrocyte to initiate a cycle of growth. it elicits a strong immune response in man; all tested adult sera from endemic areas have antibodies against this protein. our evidence indicates that ... | 1984 | 6090934 |
| human antisera detect a plasmodium falciparum genomic clone encoding a nonapeptide repeat. | plasmodium falciparum causes malaria infections in its human host. its wide distribution in tropical countries is a major world health problem. before a vaccine can be produced, the identification and characterization of parasite antigens is necessary. this can be achieved by the cloning and subsequent analysis of genes coding for parasite antigens. recently established cdna banks allow the expression of cdna derived from the simian parasite plasmodium knowlesi and p. falciparum in escherichia c ... | 1984 | 6090935 |
| isoenzyme characterization of chloroquine-resistant isolates of plasmodium falciparum from burma. | sixty isolates of plasmodium falciparum collected from hospital in-patients in rangoon and out-patients from villages near rangoon were tested in vitro for chloroquine sensitivity and then cultured to carry out starch gel electrophoresis of the following parasite enzyme: glucose phosphate isomerase (gpi) (ec.5.3.1.9.), nadp-dependent glutamate dehydrogenase (gdh) ec.1.4.1.4), lactate dehydrogenase (ldh) (ec.1.1.1.27) and 6-phosphogluconate dehydrogenase (6pgd) (e.c.1.1.1.44). variations were obs ... | 1984 | 6091301 |
| burkitt's lymphoma: clues to the role of malaria. | 1984 | 6095098 | |
| t-cell control of epstein-barr virus-infected b cells is lost during p. falciparum malaria. | endemic burkitt's lymphoma, a tumour of children in which b lymphocytes are infected with epstein-barr virus (ebv), is common in areas of africa where malaria is holoendemic. the tumour is characterized by chromosome translocations; usually the terminal portion of chromosome 8 containing the c-myc gene is translocated to chromosome 14, near the enhancer of the immunoglobulin heavy-chain locus. less frequent are translocations of chromosome 8 to the kappa light-chain locus of chromosome 2 or to t ... | 1984 | 6095104 |
| malaria antibody levels in patients with nasopharyngeal carcinoma. | the antibody titres to p. falciparum and epstein-barr virus-associated antigens were assayed in 22 patients with npc and 43 controls. all, but one patient had antimalarial titres; 14 had titres greater than 80 and 4 patients greater than 640. compared to controls the mean anti-malarial titre for most age groups were higher in the patients. those patients with high anti-malarial titres also had high iga anti-vca titre, an antibody which has been demonstrated to be diagnostic for npc. the peak ant ... | 1984 | 6095462 |
| a simple method for isolating viable mature parasites of plasmodium falciparum from cultures. | mature asexual parasites from cultures of knobby or knobless clones of p. falciparum containing 5 to 10% late forms were harvested by layering up to 10(9) erythrocytes on 3 ml percoll. the density of the percoll was adjusted to between 1.081 and 1.091 g cm-3, depending on the maturity of the parasites. centrifugation at 1500 g for 10 min produced a sharp band at the interface containing mature parasites with a purity averaging 86%. washed parasites derived from the layer or pellet showed good vi ... | 1984 | 6095494 |
| plasmodium falciparum sensitivity to chloroquine in the buyo region, ivory coast. | as part of a project, supported by the undp/world bank/who special programme for research and training in tropical diseases, for achieving a reduction of mortality due to plasmodium falciparum by means of a primary health care programme, in vivo and in vitro sensitivity testing of p. falciparum to chloroquine was carried out in the buyo region of the ivory coast. blood samples from a total of 595 children aged 2-13 years from 5 villages were screened microscopically and 32 of these children were ... | 1984 | 6096037 |
| etiology of endemic burkitt's lymphoma. | there is a considerable volume of evidence linking epstein-barr virus (ebv) etiologically with burkitt's lymphoma (bl). bl has satisfied the henle-koch criteria. thus bl patients have significantly higher ebv antibody titres than normal or tumour controls. ebv dna and ebv-determined nuclear antigen (ebna) have been demonstrated in a high proportion (greater than 90%) of endemic bl tissues. ebv can transform and immortalize human b-lymphocytes and is known to cause lymphoreticular tumours in new ... | 1984 | 6100285 |
| [plasmodium falciparum and drug resistance]. | 1984 | 6398671 | |
| [4-aminoquinoline-sensitivity of plasmodium falciparum in madagascar. i. study in two regions of the west coast]. | two three weeks field studies were performed in morondava (west coast) and nosy be (north west coast) respectively in january and april 1983. among 110 malarial patients recruited through passive detection, 70 in vivo tests and 37 in vitro tests were performed. in vivo, within 7 days 10 mg/kg chloroquine failed in 8 of the 21 treated patients, 25 mg/kg chloroquine failed in 1/43 and none of these patients treated with 10 mg/kg amodiaquine failed. in vitro, 5 among 37 isolates had chloroquine ic5 ... | 1984 | 6398672 |
| [4-aminoquinoline-sensitivity of plasmodium falciparum in madagascar. ii. study in two regions of the east coast]. | in manakara and foulpointe (east coast of madagascar) 184 malarial patients were selected among 742 patients attending dispensaries. the 7 days in vivo tests was performed in 144 patients. 13/36 failure to alternative (10 mg/kg) and 1/61 failure to standard (25 mg/kg) chloroquine who tests were observed. 10 mg/kg oral amodiaquine failed within 7 days in 5/47 patients. the in vitro semi-microtest was performed with 27 isolates. 2/24 plasmodium falciparum isolates were chloroquino-resistant (ic50 ... | 1984 | 6398673 |
| [4-aminoquinoline-sensitivity of plasmodium falciparum in madagascar. iii. studies in three regions of the interior]. | among 273 malarial patients recruited through passive detection, 177 in vivo tests were performed in andekaleka and ankazobe and 23 in vitro tests in ifanadiana, and ankazobe (inland of madagascar). in vivo, chloroquine failed within 7 days at 10 mg/kg in 28/86 patients, at 25 mg/kg in 7/34 and amodiaquine failed in 5/55 with 10 mg/kg and in 0/14 with 25 mg/kg. in vitro, the semi-microtest revealed resistance to chloroquine of 1/23 isolates (ic50 = 750 nm/1) but high sensitivity to amodiaquine, ... | 1984 | 6398674 |
| [4-aminoquinoline-sensitivity of plasmodium falciparum in madagascar: comparison of results and epidemiological perspectives]. | 1984 | 6398675 | |
| [drug sensitivity of plasmodium falciparum in madagascar. v. study of the in vitro activity of quinine, mefloquine and halofantrine]. | in a nine month fields study in 1983 in madagascar, in vitro sensitivity to others drugs than 4 aminoquinolines were tested for 39 plasmodium falciparum isolates, including 2 chloroquine resistant ones. sensitivity to quinine, mefloquine and halofantrine were recorded from 28, 39 and 11 of these isolates respectively. ic50 with semi-microtest were under 20 nm/1 for mefloquine, under 12 nm/1 for halofantrine and under 560 nm/1 for quinine. all these values are under maximal values observed elsewh ... | 1984 | 6398676 |
| chloroquine resistant plasmodium falciparum in sri lanka. | 1984 | 6398762 | |
| mefloquine-pyrimethamine-sulfadoxine combination delays emergence of mefloquine resistant plasmodium falciparum in continuous culture. | the efficacy of mefloquine against plasmodium falciparum in continuous culture was studied. the development of mefloquine resistance in p. falciparum was significantly inhibited by a combination of mefloquine, pyrimethamine and sulfadoxine. by contrast, more resistant variants were selected in continuous culture without drug pressure or with pyrimethamine-sulfadoxine pressure. the most mefloquine resistant variants were selected by step-wise increases in mefloquine pressure. | 1984 | 6398918 |
| chloroquine resistant plasmodium falciparum malaria in a local kenyan: a case report. | 1984 | 6399023 | |
| a study on the operational correlates of persisting malaria in shankergarh block of district allahabad. | 1984 | 6399059 | |
| a modified field incubator for conducting micro in vitro tests to measure drug susceptibility of plasmodium falciparum. | 1984 | 6399063 | |
| chloroquine-resistant plasmodium falciparum malaria in the netherlands. | 1984 | 6399178 | |
| the treatment of malaria. a dutch consensus. | 1984 | 6399179 | |
| development of malaria vaccines reviewed. | 1984 | 6399180 | |
| chloroquine resistance of plasmodium falciparum in sonapur phc area (assam) detected by micro in vitro and in vivo tests. | 1984 | 6399258 | |
| chloroquine resistant p. falciparum malaria in assam and meghalaya. | 1984 | 6399261 | |
| chemotherapy of malaria and the host-parasite interaction. | 1984 | 6399262 | |
| which way now for malaria control? | 1984 | 6399426 | |
| clinical pattern and therapeutic evaluation of falciparum malaria in tanzanian children. | 1984 | 6399493 | |
| plasmodium falciparum complementary dna clones expressed in escherichia coli encode many distinct antigens. | a library of cdna clones expressing antigens of the asexual blood-stages of plasmodium falciparum (isolate fcq27/png) was constructed in the bacteriophage vector gamma gt11-amp3. clones expressing p. falciparum antigens (as polypeptides fused to beta-galactosidase) were selected by their reactivity in an in situ colony immunoassay with affinity-purified malaria antibodies. a detailed analysis of 78 antigen-positive clones selected from approximately 10,000 recombinant clones has shown them to co ... | 1984 | 6399547 |
| characterisation of monoclonal antibodies against blood forms of plasmodium falciparum by the immunofluorescent test. | 1984 | 6397210 | |
| a review of present antimalaria activities in africa. | in the who african region, malaria still remains a public health problem that constitutes a serious obstacle to development. this is due to the high intensity of transmission and the lack of sufficient resources to apply effective methods of control. however, the recent adoption of the primary health care delivery system offers a new basis for antimalaria strategies, and this has led to renewed hope of (1) reducing mortality and morbidity, (2) minimizing harmful effects on socioeconomic developm ... | 1984 | 6397273 |
| lessons learned from applied field research activities in africa during the malaria eradication era. | the malaria conference in equatorial africa, convened by the world health organization in 1950 in kampala, uganda, was a milestone in the history of modern malaria control activities on the continent of africa. it presented and assessed the available international information on epidemiological aspects of this disease and attempted to coordinate the various methods of research and control of malaria. its two main recommendations were that malaria should be controlled by all available methods, ir ... | 1984 | 6397274 |
| recent applied field research activities carried out in tropical africa. | a review has been undertaken of applied field research in malaria in tropical africa from 1975 onwards, the aim being to show recent trends and to emphasize the needs for further research in support of malaria control.studies are grouped according to whether they relate to parasites, vectors, epidemiology, or control. the first group is concerned mainly with the study of the appearance and development of resistance of plasmodium falciparum to drugs. the second group deals with vector bionomics a ... | 1984 | 6397275 |
| the impact of malaria chemoprophylaxis in africa with special reference to madagascar, cameroon, and senegal. | some past and present experiences in the use of antimalarial drugs, particularly for chemoprophylaxis, are reviewed. the failure in the long term of mass chemoprophylaxis with weekly chloroquine in children in madagascar, cameroon, and senegal is discussed, the reasons for failure being an increasing lack of resources to ensure regular drug distribution and lack of supervision of dosage.the increasing number of reports confirming chloroquine resistance from east africa over the last decade poses ... | 1984 | 6397276 |