Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [histological, histoenzymatic and biochemical study of the liver and small intestine of rats during short periods of starvation]. | the structure and the activity of acid phosphatase, beta-glucuronidase, alkaline phosphatase and atp-ase in the liver and small intestine of rats receiving for 20 days a one-time, fixed at a certain time (2 o'clock) feeding was studied morphologically in dynamics in 2, 6, 24 and 48 hours after the last feeding. furthermore, parallel with this the activity of acid phosphatase, beta-glucuronidase, alpha-glucosidase and beta-acetylglucosaminidase was determined in homogenates by biochemical methods ... | 1975 | 128202 |
| the effect of hormone induced stress upon the extent of alkylation of rat liver nucleic acids by n-methyl-n-nitrosourea. | an examination was made of the effect of treatment with methylating agents of varying carcinogenic potency and with stress inducing hormones upon dna synthesis in the resting liver of the rat. with the methylating agents an early stimulation of dna synthesis was observed, but this was depressed below the control levels at later times and with higher doses; hormone administration also resulted in a depression of dna synthesis but, without any initial stimulation at the dosage employed. under cond ... | 1975 | 128216 |
| bacteriophage p13234mo-mediated intra- and intergroup transduction of antibiotic resistance among streptococci. | numerous streptococcal strains representing serogroups a, c, and g were tested for transducibility to antibiotic resistance by the temperate group a streptococcal phage p13234mo. the resistance markers carried by group a donor strains included the erl1 factor determining inducible resistance to erythromycin, lincomycin and streptogramin-b type antibiotics as well as chromosomally located mutations for streptomycin and spectinomycin resistance. erl1, presumably of plasmid nature, was transduced t ... | 1975 | 128266 |
| brain and spinal cord lesions in pigs inoculated with swine vesicular disease (ukg strain) virus and coxsackievirus b5. | pigs inoculated intravenously with swine vesicular disease virus (ukg strain), those inoculated with coxsackievirus b5, and other pigs exposed by pen contact to the same viruses developed diffuse encephalomyelitis. perivascular cuffing, with lymphocytes and formation of neuroglia cell foci, were most prominent in telencephalon, diencephalon, and mesencephalon. encephalitis was of mild to severe intensity. severity of lesions was more extensive and severe in the pigs exposed to swine vesicular di ... | 1975 | 128305 |
| (+)-catechin effects on x-rays irradiated skin of rats. | the effect of (+)-catechin on the skin of rats previously irradiated was a net decrease of amps content and a partial recovery of the insoluble collagen-loss due to x-rays. | 1975 | 128328 |
| editorial: subunits of myosin. relations to atpase activity and mechanical function of muscle. | under certain conditions the specific atpase activity of myosin of a given muscle can be altered. the cause of this alteration can only lie in the myosin molecule itself. to produce an enzymatic activity of myosin, an interaction between their light and heavy chains is necessary. however, the specific activity appears to be determined mainly by light chains. hence, one ought also to look for a basis of the changed activity in changes of the subunits of myosin. there are strong indications that t ... | 1975 | 128346 |
| [effect of glycerol on monovalent cation interactions with (na+-k+)-atpase of human solubilized erythrocyte membranes]. | 1975 | 130942 | |
| computerized axial tomography in syringomyelia. | the acta-scanner, a device for computerized axial tomography, permits cross-sectional radiographic study of the entire human body, including the spine. in the acta-scan, the spinal cord appears as a roundish formation surrounded by the less dense subarachnoidal cerebrospinal fluid. the spines of 18 patients with verified (nine cases) or suspected (nine cases) syringomyelia were studied by acta-scanning. in seven of the verified and in four of the nonverified cases, some evidence of cord cavitati ... | 1975 | 162787 |
| thermodynamics of complex formation between nicotinamide adenine dinucleotide and pig skeletal muscle lactate dehydrogenase. | formation of the binary complex between the reduced coenzyme nicotinamide adenine dinucleotide (nadh) and pig skeletal muscle lactate dehydrogenase (ldh, ec 1.1.1.27) has been investigated by calorimetric and equilibrium dialysis techniques in 0.2 m potassium phosphate buffer (ph 7.0) at various temperatures. analysis of thermal titration curves at two temperatures (25 and 31.5 degrees) shows that the experimental enthalpy data can be rationalized assuming four independent and equivalent binding ... | 1975 | 162832 |
| septic and crystalline joint disease. a simultaneous occurrence. | bacteria and crystals were simultaneously recovered from the synovial fluid of two patients with acutely inflamed joints. the bacteria were initially identified on gram stain and subsequently grown on supplemented peptone broth, and the crystals were readily identified by polarizing microscopy. although cause-and-effect relationship between these two types of arthritis cannot be established, the need for thorough examination of synovial fluid is evident. | 1975 | 162966 |
| autoradiographic techniques for localizing steroid hormones. | 1975 | 162975 | |
| the tecumseh study of respiratory illness. viii. acute infection in chronic respiratory disease and comparison groups. | individuals with chronic lung disease and their families were selected from the tecumsch community along with similarly selected families as comparison groups and studied for 1-year periods. occurence of acute respiratory illness was ascertained weekly by telephone and calculated as an annual rate. persons with chronic bronchitis not only experienced more acute lower respiratory illness than healthy comparison subjects, but total illness rates were somewhat higher as well. infection rates were d ... | 1975 | 163065 |
| modifications in soluble protein kinase and cyclic-amp binding capacity of developing rat brain. | 1975 | 163291 | |
| studies of polyoma virus dna: cleavage map of the polyoma virus genome. | a small-plaque polyoma virus, mpc-1, was isolated from a mouse plasmacytoma. the dna of this polyoma virus was cleaved with a restriction enzyme from haemophilus influenzae (hin d), and the molecular weights of the limit products were analyzed by electrophoresis and electron microscopy. the fragments produced by this enzyme have been ordered by analysis of partial digest products. a physical map of the polyoma virus genome was then constructed. | 1975 | 163343 |
| posttranscriptional selection of simian virus 40-specific rna. | analysis of the viral-specific rna in simian virus 40(sv40)-infected monkey kidney cells indicated the extensive transcription of both dna strands. these symmetrically transcribed sequences were localized in the nucleus of infected cells, whereas only the "true" early and late sv40 transcripts were found in the cytoplasm. these results suggest that selective posttranscriptional degradation and/or transport occurs after transcription of the viral dna. on the basis of hybridization experiments wit ... | 1975 | 163366 |
| ornithine decarboxylase activity: control by cyclic nucleotides. | both exposure to cold and administration of aminophylline result in rapid increases in cyclic adenosine monophosphate (cyclic amp) in the adrenal medulla and adrenal cortex. these increases are followed by dramatic increases in ornithine decarboxylase activity is due to new enzyme systhesis. the data suggest that the decarboxylase activity is regulated by an increase in cyclic amp. | 1975 | 163486 |
| sites in the polyoma genome cleaved by restriction endonuclease hindii. | 1975 | 163542 | |
| microbial assimilation of hydrocarbons. i. the fine-structure of a hydrocarbon oxidizing acinetobacter sp. | 1. the fine-structure analysis of the hydrocarbon oxidizing microorganism, acinetobacter sp., demonstrated a cytoplasmic modification resulting from growth on paraffinic and olefinic hydrocarbons. 2. intracytoplasmic hydrocarbon inclusions were documented by electron microscopy with chemical identifications obtained by gas chromatography and x-ray diffraction. 3. these results demonstrate the ability of a microorganism to accumulate hydrocarbon substrates intracellularly which, in turn, indicate ... | 1975 | 163624 |
| activation of 3',5'-cyclic adenosine monophosphate phosphodiesterase by calcium ion and a protein activator. | 3',5'-camp phosphodiesterase was partially purified from bovine cerebral cortex. a heat-stable activating factor was separated from the enzyme by chromatography on deae-cellulose. the enzyme in crude ammonium sulfate fractions was stimulated by 5 mm cacl2. this stimulation was reversed by the calcium chelator egta. the main phosphodiesterase peak obtained by deae-cellulose chromatography was not stimulated by ca2+. upon addition of column effluent containing a heat stable factor, ca2+ activation ... | 1975 | 163641 |
| cellulase location in cellvibrio fulvus. | the location of cellulase in c. fulvus depends on the carbon source for growth and the age of the culture. when cells were grown on glucose or cellobiose all cmc-hydrolyzing enzyme was cell-bound but only part of the activity was located on the cell surface. treatment of cells with edta, lysozyme, and detergents and subsequent fractionation experiments showed that cellulase was also located in the periplasm and bound to a membrane fraction. growth on cellulose gave cell-free cellulase active aga ... | 1975 | 163668 |
| antigenic relationship between medullary thymocytes and a subpopulation of peripheral t cells in the rat: description of a masked antigen. | 1975 | 163704 | |
| recovery of b lymphocyte responsiveness after complete radioactive antigen suicide, and the affinity of antibody made after incomplete suicide. | 1975 | 163705 | |
| rat sertoli cells: a rapid method for obtaining viable cells. | a method is described for obtaining populations of viable sertoli cells from rat testes. minced whole testes from rats of 15 to 29 days of age are sequentially treated with collagenase and pancreatin. the resulting suspension of cells is sedimented through a sucrose density gradient. preparations are produced consisting of from 60% to 82% sertoli cells, an enrichment of 2 to 5 times the proportion of sertoli cells in whole testes of these ages. the preparations are free of interstitial cells, ar ... | 1975 | 163729 |
| inhibition by iodide of the activation of the thyroid cyclic 3',5'-amp system. | the action of iodide on the cyclic amp system of dog thyroid slices has been studied. iodide inhibits the enhancement of cyclic amp accumulation in the presence of tsh. such an effect is also observed in horse, beef and sheep thyroid slices, but not in dog kidney slices stimulated by parathyroid hormone or in rat parotid slices stimulated by isoproterenol. the effect in dog thyroid slices is suppressed by 1mm naclo4, 1mm methimazole and 1mm propylthiouracil. similar data have been obtained for p ... | 1975 | 163738 |
| experimental intracerebral movement of electron microscopic tracers of various molecular sizes. | the authors demonstrate that, while the intracerebral movement of proteins and other relatively large molecules is restricted by tight junctions of endothelia and epithelia of the brain, calcium ion penetrates these barriers and moves between the blood and csf cavities by extracellular pathways. mechanisms underlying csf formation are considered on the basis of this anatomical continuity of the vascular, extracellular, and csf compartments of the brain for very small molecules. | 1975 | 163897 |
| nongenetic complementation of group v temperature-sensitive mutants of vesicular stomatitis virus by uv-irradiated virus. | cells infected with temperature-sensitive (ts) mutants of complementation group v of vesicular stomatitis virus (vsv) give an enhanced yield at nonpermissive temperature when co-infected or superinfected with uv-irradiated virus. virions produced in these mixed infections are temperature sensitive and do not complement ts v45. rescue of group v mutants is multiplicity dependent. it can occur in the presence of cycloheximide; kinetics of rescue are similar in the absence or in the presence of the ... | 1975 | 163919 |
| anti-inflammatory steroids and collagen metabolism: glucocorticoid-mediated decrease of prolyl hydroxylase. | 1975 | 163951 | |
| summary and discussion of future applications of vaccination and immunotherapy. | 1975 | 163956 | |
| letter: eb-virus-seropostivie person is susceptible to infectious mononucleosis. | 1975 | 163963 | |
| assembly of viruses. | 1975 | 164048 | |
| [change in the glycolytic and glucose-6-phosphate dehydrogenase activity in experimental cirrhosis of the liver]. | development of cirrhosis of liver tissue did not influence the intensity of glycolysis, with glucose as a substrate, in supernatant fraction of liver homogenate in chronic intoxication with ccl4. in preparations of cirrhotic liver, as compared with liver from the intact animals, more distinct activation of glycolysis was caused by addition of atp and nad at the stage of 3-week intoxication and also by addition of hexokinase, glyceraldehydephosphate dehydrogenase and lactate dehydrogenase at the ... | 1975 | 164085 |
| an effect of glucagon on 3', 5'-cyclic amp phosphodiesterase activity in isolated rat hepatocytes. | 1975 | 164185 | |
| spin label and lanthanide binding sites on glyceraldehyde-3-phosphate dehydrogenase. | the electron spin resonance spectrum of rabbit muscle d-glyceraldehyde-3-phosphate dehydrogenase spin-labelled with 4-(2-iodoacetamido)-2,2,6,6-tetramethylpiperidinooxyl has two components. one component is due to a spin label highly immobilized on the enzyme surface and the other to a nitroxyl group able to tumble more rapidly. the spin-labelled enzyme is inactive. selective modification of the active site cysteine residue (149) and determinations of total sulphydryl content implicate this resi ... | 1975 | 164224 |
| responses of electrical potential, potassium levels, and oxidative metabolic activity of the cerebral neocortex of cats. | we measured simultaneously the oxidative metabolic activity, monitored as the tissue fluorescence attribute to intramitochondrial nadh, the extracellular potassium level with ion-selective microelectrodes, and the focal extracellular electrical potential, of one site in intact cerebral cortex of cats. when the cerebral was stimulated by trains of repeated electric pulses applied either directly to its surface or to an afferent pathway, the corrected cortical fluorescence (f-r) declined indicatin ... | 1975 | 164265 |
| sero-immunity to poliovirus in children and young women: england 1972-4. | a total of 292 children's sera and 706 antenatal sera from different parts of england were tested for the presence of poliovirus neutralizing antibodies. little vairation was found between different areas and types of community, but a lower porportion of the 5-14 year old children had antibody than younger children and young adult women. the proportion of the young population with antibody, and the current acceptance rates for oral poliovirus vaccine are barely satisfactory. | 1975 | 164503 |
| the dopamine vascular receptor. | 1975 | 164871 | |
| pharmacological reversal of cholestasis-associated decrease in hepatic cytochrome p-450. | 1975 | 164874 | |
| purification of the sex steroid binding protein from human serum. | the sex steroid binding protein from human pregnancy serum was purified to homogeneity by affinity chromatography and preparative polyacrylamide gel electrophoresis. the selective adsorbants were prepared by coupling [3h]-5alpha-dihydrotestosterone 17beta-hemisuccinate to 3,3'-diaminodipropylamine-agarose, poly(lys-dlala)-agarose, and albumin-agarose. the most effective adsorbant purifying for the binding protein was 5alpha-dihydrotestosterone 17beta-hemisuccinyl-3,3'-diaminodipropylamine-agaros ... | 1975 | 164886 |
| studies on interaction between histone v (f2c) and deoxyribonucleic acids. | histone v (2fc) from chick erythroctes was used in the study of its interaction with dna from various sources. complexes between this histone and dna were formed using the procedure of continuous nacl gradient dialysis in urea. two physical methods, namely thermal denaturation and circular dichroism (cd), were used as analytical tools. thermal denaturation of nucleohistone v with chick or calf thymus dna shows three melting bands: band i at 45-50 degrees corresponds to free base pairs; band ii a ... | 1975 | 164887 |
| the assay and isolation of dna rings using an atp-dependent endonuclease. | the atp-dependent endonuclease from hemophilus influenzae is relatively inactive on closed or open dna rings, yet rapidly hydrolyzes single- or double-chained linear dna. this enzyme in combination with an exonuclease (exo vii) has been shown to spare various circular dna molecules including those having single-chain regions of significant length. however, rings containing single-chained regions are broken at a rate depending on the length of these regions. by admixing a linear dna of alternate ... | 1975 | 164888 |
| cyclic adenosine 3':5'-monophosphate and inhibition of deoxyribonucleic acid synthesis in vitro. | results are presented which suggest that inhibition of tritiated thymidine incorporation into the deoxyribonucleic acid of bone marrow in vitro by high concentrations of cyclic adenosine 3':5'-monophosphate is pharmacological in nature, and thus apparently unrelated to the physiological actions of camp. | 1975 | 165140 |
| isolation and characterization of mutants of haemophilus influenzae deficient in an adenosine 5'-triphosphate-dependent deoxyribonuclease activity. | by a direct assay approach, mutants of haemophilus influenzae rd that are deficient in adenosine 5'-triphosphate-dependent deoxyribonuclease activity (add-) were isolated and characterized. a large proportion (50 to 90%) of the cells in cultures of these mutants failed to produce visible colonies when plated. an extensive analysis of the recombination proficiency of these strains revealed that the transformation frequency (transformants per competent cell) in the mutants was similar to that foun ... | 1975 | 165169 |
| mutants of neurospora deficient in nicotinamide adenine dinucleotide (phosphate) glycohydrolase. | a new screening technique has been developed for the rapid identification of neurospora crassa mutants that are deficient in nicotinamide adenine dinucleotide glycohydrolase (nadase) and nicotinamide adenine dinucleotide phosphate glycohydrolase (nadpase) activities. using this procedure, five single-gene mutants were isolated whose singular difference from wild type appeared to be the absence of nad(p)ase (ec 3.2.2.6). all five mutants were found to be genetically allelic and did not complement ... | 1975 | 165174 |
| colchicine resistance in mammalian cell lines. | colchicine-resistant variants derived from mouse and syrian hamster lines are described. the resistant cells do not appear to be true mutants, since they appear at a high frequency, unaffected by treatment with ethyl methyl sulphonate, and are unstable in the absence of the drug. they are cross-resistant to other drugs, show a reduced rate of binding of colchicine in monolayer, and give extracts with colchicine-binding properties identical to those of the wild type. thus the resistance is due to ... | 1975 | 165207 |
| the effect of melatonin on steroidogenesis by the human ovary in vitro. | melatonin stimulated steroidogenesis in two compartments of the human ovary in vitro. in a corpus luteum of the menstrual cycle, melatonin increased progesterone synthesis in a dose related manner. both serotonin and n-acetyl serotonin had no effect on progesterone synthesis. in the ovarian stroma, melatonin stimulated the incorporation of acetate-1-14-c into androstenedione. binding of radioactive hcg by the corpus luteum was unaffected by melatonin. no specific binding of radioactive melatonin ... | 1975 | 165215 |
| carcinogenicity of 5-nitrofurans and related compounds with amino-heterocyclic substituents. | carcinogenicity of eight 5-nitrofurans with heterocyclic substituents at the 2-position of the furan ring was investigated by feeding the chemicals to sprague-dawley female rats. n-[5-(5-nitro-2-furyl)-1,3,4-thiadiazol-2-yl]acetamide induced in 30 rats the highest incidence of tumors with the greatest number of tissues involved: forestomach squamous cell tumors (22), kidney pelvis transitional cell carcinomas (15), pulmonary alveolar cell carcinomas (16), hemangioendothelialsarcomas (20) of the ... | 1975 | 165300 |
| oncogenic transformation of murine lymphoid cells by in vitro infection with abelson leukemia virus. | spleen cell cultures stimulated to dna synthesis by antigen or mitogen were infected with abelson virus, a c-type rna virus inducing nonthymic lymphomas in mice. after 3 days the cells were transferred to mice and caused 100 percent incidence of lymphomas in as few as 29 days. that a number of the tumors were of donor origin, as shown by female karyotypes in recipient male mice, indicated that cells infected by virus in vitro were transformed. the process depended upon both virus and stimulation ... | 1975 | 165315 |
| the last conversation with dr. earl w. sutherland, jr: the feedback regulation of cyclic nucleotides. | 1975 | 165349 | |
| letter: petechiae: due to adenovirus type 7 infection or to acetylsalicylic acid therapy? | 1975 | 165451 | |
| the inhibitory effects of mgcl2 on the inactivation kinetics of poliovirus by urea. | by analyzing the inhibitory effects of mg-2+ on the three-stage urea inactivation curve of poliovirus, it was concluded that urea inactivates poliovirus via a two-step reaction as follows: urea initially converts the native virus into an intermediate state which is still infectious but is now highly sensitive to inactivation. this reaction is unaffected by mg-2+ and is reversible. in the subsequent reaction, the sensitized virus is either irreversibly inactivated by urea or reversibly stablized ... | 1975 | 165542 |
| the measurement of choline kinase activity in rat brain: the problem of alternate pathways of atp metabolism. | 1975 | 165749 | |
| the occurrence of the pyruvate dehydrogenase complex and the pyruvate-ferredoxin oxidoreductase in blue-green bacteria. | 1975 | 165997 | |
| uptake and binding of labeled corticosterone by the salt gland of the duck (anas platyrhynchos). | 1975 | 166016 | |
| effects of 5-fluorouracil on exocrine glands. iii. fine structure of brunner's glands of rats. | effects of a pyrimidine analogue, 5-fluorouracil (fur), have been studied by electron microscopy and by electron microscopic cytochemical techniques. previous studies have demonstrated that rats show serious gastrointestinal disturbances 5 days after 3 daily injections of fur (50 mg/kg). the present investigation demonstrates that brunner's glands under the same conditions suffer certain cytological changes involving the golgi apparatus, where a notable reduction in the number of golgi stacks is ... | 1975 | 166061 |
| effects of trigeminal tractotomy and of carbamazepine on single trigeminal sensory neurons in cats. | 1975 | 166097 | |
| pharmacology and therapeutics of bone. | 1975 | 166098 | |
| the influence of sodium, potassium and lanthanum on amino acid release from spinal-medullary synaptosomes. | 1975 | 166144 | |
| cyclic amp metabolism and nucleic acid content in the lymphocytes of the thymus, spleen, and lymph nodes of protein-deficient rats. | the concentration of cyclic amp (camp) and its metabolites (5'-amp and adenosine) as well as the adenyl cyclase, camp phosphodiesterase, and 5'-nucleotidase activities were determined in lymphocytes of thymus, spleen, and lymph nodes of control and protein-deficient rats. the values of these parameters, when expressed as per milligran dna and as per 10-8 cells, but not always when expressed as per milligran protein, were much lower in the thymus as compared with the spleen and the lymph nodes in ... | 1975 | 166150 |
| isolation of a glycogen synthase i kinase that is independent of adenosine 3':5'-monophosphate. | three protein kinases (atp:protein phosphotransferase, ec 2.7.1.37) were detected when the soluble fraction of rabbit kidney medulla was chromatographed on deae-cellulose with a linear nac1 gradient. the first two kinases eluted (peak 1 and peak ii) were cyclic-amp-dependent, wheras peak iii was cyclic-amp-independent. a procedure was developed to separate the catalytic subunit of peak ii cyclic-amp-dependent protein kinase (representing the bulk of the histone kinase activity) from peak iii pro ... | 1975 | 166380 |
| a new method of diagnosing myocardial contusion in man by radionuclide imaging. | a new method of diagnosing myocardial contusion was studied in 8 patients in whom the injured mycardium was visualized as an abnormal area of increased activity in the region of the heart one hour after intravenous injection of 10 mci of 99m-tc-sn-polyphosphate or pyrophosphate. serum enzymes in these patients were elevated, but electrocardiograms were nonspecific for myocardial injury. it is hoped that this new technique of imaging the injured myocardium will provide specific and confirmatory d ... | 1975 | 166410 |
| effect of citrate and aminotriazole on matrical plates induced in hepatic microbodies. | feeding of acetylsalicylic acid (asa), clofibrate and dimethrin induces formation of matrical plates in heptaic microbodies. administration of sodium citrate prevents formation of matrical plates in rats fed asa, but not in rats treated with clofibrate or dimethrin. propionate has a similar effect on rats fed asa but not those fed clofibrate. feeding of aminotriazole prevents formation of matrical plates in rats fed asa without inhibiting the proliferation of microbodies. sodium citrate and amin ... | 1975 | 166481 |
| sequences of oligonucleotides prepared from tobacco mosaic virus ribonucleic acid. | 1975 | 166485 | |
| tandem repetition of the origin of dna replication in defective polyoma virus dna's. | 1975 | 166489 | |
| a rapid purification method of restriction endonucleases from haemophilus strains. | a simple and rapid method of purification of restriction endonucleases from different haemophilus strains is presented. by this method highly purified and stable enzymes can be obtained. separation of different restriction activities present in the same strain is possible. this method was so far successfully used with haemophilus influenzae, haemophilus parainfluenzae and haemophilus aegyptius strains. the main advantages over previously published procedures reside in the simplication of certain ... | 1975 | 166680 |
| inhibition by somatostatin of glucose induced 3':5'-monophsophate (cyclic amp) accumulation and insulin release in isolated pancreatic islets of the rat. | 1975 | 166881 | |
| the physicochemical properties of the cytoplasmic androgen receptor in the kidneys of normal, carrier female (tfm/+) and androgen-insensitive (tfm/y) mice. | the physicochemical properties of the androgen-receptor complex in mouse kidney were determined. the sedimentation coefficient, 7.9s and stokes radius of 82a, are compatible with an asymmetric protein [frictional ratio f/fo 1.98; axial ratio, assuming a prolate ellipsoid, 20] having a molecular weight of 270,000 daltons. the kidney receptor is a relatively acidic protein of esoelectric point (pi) 4.8 and readily precipitable with protamine sulfate or ammonium sulfate. studies with protein-specif ... | 1975 | 168054 |
| anatomy of herpes simplex virus dna: strain differences and heterogeneity in the locations of restriction endonuclease cleavage sites. | digestion of herpes simplex virus dna by the hiniii or eco ri restriction endonucleases yielded 11 to 15 fragments with molecular weights between 1 x 10(6) and 28 x10(6). the electrophoretic profiles obtained in 0.3% agarose gels with dna fragments from none different strains of herpes simplex virus type 1 could be readily differentiated from the patterns exhibited by the corresponding fragments from four separate strains of type 2 virus; however, with each serotype, the laboratory strains diffe ... | 1975 | 168574 |
| simian virus 40 dna directs synthesis of authentic viral polypeptides in a linked transcription-translation cell-free system. | a linked cell-free system has been developed which is capable of transcribing and translating mamalian viral dna, and its characteristics and requirements are outlined. in this system, simian virus 40 (sv40) dna form i (supercoiled) directed the synthesis of discrete polypeptides up to 85,000 daltons in size. one of these products was indistingusihable from authentic major virus capsid protein vpi, as judged by mobility on sodium dodecyl sulfate/polyacrylamide gels, antibody predipitation, and p ... | 1975 | 168582 |
| antibody levels to parainfluenza, rubella, measles, and influenza a virus in children with polymyositis. | the cf and hi antibody titers to rubella and measles viruses, the cf titers to influenxa a, and the hi titers to parainfluenza 1, 2, and 3 were carried out on the sera of 20 patients with childhood polymyositis and their matched controls. the titers for measles, parainfluenxa 1, and influenza a were slightly higher for patients than for controls. the control group had antibody titers to rubella and parainfluenza 2 and 3 higher than or similar to those of patients. strong patterns or significant ... | 1975 | 168906 |
| an unusual beta-cell adenoma. | an unusual case of insulinoma in a 51-year-old man demonstrates the difficulties in diagnosing and treating hypoglycemia. from the many laboratory tests and procedures performed, the only findings typical of insulinoma were inappropriate values of plasma immunoreactive insulin in relation to the corresponding values of blood glucose, and these were sporadic. a small beta-cell adenoma in the pancreatic head was the cause of the hypoglycemia. | 1975 | 168953 |
| phenotypically mixed vesicular stomatitis virus particles produced in human tumor cell lines. | 1975 | 169045 | |
| effect of some phosphodiesterase inhibitors on two different preparations of adenosine 3',5'-monophosphate phosphodiesterase. | 1975 | 169139 | |
| denaturation map of polyoma dna. | a denaturation map of polyoma dna cleaved by eco r1 to form linear molecules was established by electron microscopy. partial denaturation, under the same conditions, of fragments obtained by haemophilus influenzae restriction enzymes allowed us to align the denaturation map with the already established physical map of polyoma dna (griffin et al., 1974). | 1975 | 169394 |
| sinusitis of the maxillary antrum. | twenty-four adults with clinical evidence of sinusitis were studied by 65 needle punctures of the maxillary antrum. fourteen of 15 sinuses with normal transillumination and 19 of 26 that were dull had normal aspirates, whereas 24 of 24 that were opaque had abnormal aspirates (p less than 0.001). marked mucosal thickening as determined radiologically (water's view) was associated with abnormal aspirates whereas lesser mucosal thickening was not (p less than 0.001). in acute sinusitis, there was a ... | 1975 | 169466 |
| functional consequences of modifying highly reactive arginyl residues of fructose 1,6-bisphosphatase. loss of monovalent cation activation. | modification of pig kidney fructose 1,6-bisphosphatase with 2,3-butanedione (in the presence of amp) results in the loss of activation of the enzyme by monovalent cations. under these conditions about 8 arginyl residues per mole of enzyme were modified. no other residues were modified. no loss of monovalent cation activation occurs when modification with 2,3-butanedione is carried out in the presence of amp plus the substrate fructose 1,6-bisphosphate and 3.2 less arginyl residues were modified. ... | 1975 | 169892 |
| oestrogen receptors in chick oviduct. characterization and subcellular distribution. | macromolecular components with properties of oestrogen receptors have been identified in the 0.5 m kcl nuclear soluble, the nuclear insoluble and the cytosol fractions of laying hen and immature (2--4 weeks, untreated by hormone) chicken oviduct. 7n the 0.5 m kcl extract of laying hen oviduct nuclei, a receptor, of protein nature according to the effects of enzymic treatments, has been identified. it exhibits high affinity for oestradiol with an apparent equilibrium association constant ka = 4 - ... | 1975 | 170115 |
| effect of endogenous and exogenous murine leukemia virus infection on immunologic response. | 1975 | 170118 | |
| current molecular insights into the mechanism of estrogen action. | 1975 | 170147 | |
| operator-promoter functions in the threonine operon of escherichia coli. | the prophage curing properties of secondary-site lysogens of coliphage lambda have been studied. the site of integration in the original lysogen (l79) is within the ooerator-promoter region of the thr operon. as a result, expression of the thr enzymes is reduced, and the strain is a leaky threonine auxotroph. heat pulse curing of strain l79 and a thr+ lysogenic revertant (l79-20) showed that heat pulse curing of both lysogens was int and xis dependent and occurred by correct excisions of the pro ... | 1975 | 170244 |
| role for deoxyribonucleic acid ligase in deoxyribonucleic acid polymerase i-dependent repair synthesis in toluene-treated escherichia coli. | in a toluene-treated mutant of escherichia coli k-12 having a temperature-sensitive, conditionally lethal mutation in the structural gene for deoxyribonucleic acid (dna) ligase, an extensive dna repair synthesis occurred in x-irradiated cells at the nonpermissive temperature, 42 c. at the permissive temperature, 30 c, nearly normal semiconservative synthesis and limited repair synthesis were observed when dna ligase was activated by the addition of nicotinamide adenine dinucleotide. | 1975 | 170252 |
| new satellitism test for isolation and identification of haemophilus influenzae and haemophilus parainfluenzae in sputum. | a new satellitism test designed to facilitate the isolation and identification of haemophilus influenzae and haemophilus parainfluenzae is described. in the basal medium, nicotinamide adenine dinucleotide is incorporated at a concentration of 0.2 mug per ml, an amount adequate for h. influenzae but not for h. parainfluenzae. two disks are placed on the surface of the medium, one disk being impregnated with 60 mug of hemin and the other with 15 mug of nicotinamide adenine dinucleotide. under thes ... | 1975 | 170304 |
| homology and relationship between the genomes of papovaviruses, bk virus and simian virus 40. | a number of hybridization techniques have been used to assess the homology between the genomes of bk virus (bkv) and simian virus 40 (sv40). a noncontiguous set of homologous sequences has been localized primarily within the late region of the sv40 genome, and these sequences presumably account for the cross-reaction between v-antigens of the two viruses. the reason for the relatively strong crossreaction between sv40 and bkv t-antigens is still unclear. the sequence homology and similarity in g ... | 1975 | 170606 |
| [ultrastructure of human hepatocytes in early prenatal ontogenesis]. | the structure of hepatic cells was studied in 40 human embryos and fetuses from 4 to 16 weeks of intrauterine development. depending on the differentiation degree of organells and their amount in the hepatic cell of the embryo and fetus three types of hepatic cells are distinguished: slightly differentiated, transitional and differentiated cells. in the course of early prenatal ontogenesis the amount of slightly differentiated hepatic cells diminishes and the amount of transitional and different ... | 1975 | 170891 |
| mode of action of antirheumatic drugs on the cyclic 3',5'-amp regulated glycosaminoglycan secretion in fibroblasts. | the effect non-steroidal anti-inflammatory drugs on formation and release of glycosaminoglycans (gag) and cyclic 3',5'-amp levels was studied in embryonic mouse fibroblasts. the results were compared and correlated with the action of these drugs on cyclic 3',5'-amp-dependent as well as independent protein kinase obtained from bovine diaphragm. 1. phenylbutazone dose-dependently decreased cyclic 3',5'-amp levels and gag secretion both in unstimulated and pge1 stimulated cells. 2. indometacin decr ... | 1975 | 171589 |
| mapping the transcription site of the sv40-specific late 16 s mrna. | this paper describes the purification of polysomal rna from sv40-lytically infected cv1 (monkey) cells and separation of the two distinct classes of sv40-specific mrna sedimenting at 16 s and 19 s. these classes have been hybridized with the whole sv40 dna genome as well as with the sv40 hind fragments. the results have permitted the mapping of sv40-specific late 16 s mrna from approximately 0.945 to 0.175 map units. | 1975 | 171628 |
| location of histones on simian virus 40 dna. | the physical location of histone molecules in a simian virus 40 dna-histone complex isolated from purified virions was examined using site-specific restriction endonucleases. the complex contains four host histone species but lacks histone f1. histones prevent complete cleavage of sv40 dna by two restriction enzymes, hindiii and ecori. from the pattern of dna fragments resulting from cleavage of the histone-dna complex by the hindiii endonuclease, which makes six breaks on purified sv 40 dna, we ... | 1975 | 171647 |
| adenosine, amp, cyclic amp, theophylline and the action and production of erythropoietin. | erythropoiesis, as measured by the uptake of 59fe into plethoric mice, is stimulated by adenosine, amp, cyclic amp, and dibutyryl cyclic amp, but not by cytidine, its nucleotides or cyclic gmp. this stimulation is erythropoietin dependent, because it is prevented by anti-erythropoietin. theophylline neither stimulates erythropoiesis nor potentiates the action of erythropoietin on bone marrow cells in plethoric mice. theophylline does potentiate the production of erythropoietin in rats following ... | 1975 | 171682 |
| [reaction of free radicals in gamma-irradiated dna in the presence of oxygen]. | 1975 | 171702 | |
| studies on human serum high-density lipoproteins (hdl). iv. isolation of lipoprotein families after incubation of hdl. | the high-density lipoproteins (hdl) of human serum appear to be unstable and easily exposed to chemical changes during isolation. in earlier studies we have isolated and purified hdl subfractions either in the presence of an sh-blocking agent, dtnb, or in the cold. by both procedures reproducible lipoprotein subfractions could be recovered by hydroxyl apatite column chromatography at the elution steps 0.03-0.05 mol/l (subfraction ii) and 0.05-0.15 mol/l phosphate buffer (subfraction iii). the pr ... | 1975 | 171762 |
| location of the small restriction fragments, hind-l, hind-m and hpa-e, on the simian virus 40 genome. | 1975 | 171849 | |
| adult cytomegalovirus inclusion retino-uveitis. | a 27-year-old male renal transplant recipient acquired cytomegalovirus (cmv) infection of both eyes. this was characterized early by a distinctive necrotic retinitis with discrete advancing edges. a large exudative retinal detachment and hypopyon developed in one eye, and cultures from the anterior chamber aspirate grew cmv. cytomegalovirus was also isolated from the urine and throat; after serial negative base-line titers, cmv antibody titer became positive. at autopsy cmv was isolated from lun ... | 1975 | 171958 |
| on the aetiopathogenesis of rheumatoid arthritis. | 1975 | 172002 | |
| adaptive alterations in lactobacillus casei. gluconeogenesis in cells grown on ribose. | 1975 | 172026 | |
| [electron microscopy studies on proliferation of foot-and-mouth disease virus in cell cultures. i. morphologic changes in cell nucleus]. | the first morphological indication of fmd infection of a cell culture was in the nucleus. components of nucleoli became segregated and were finally present only as remnants. it was not possible to distinguish different stages of segregation, as in the case of entero-virus infections, because of the rapidity of fmd virus proliferation. following changes in nucleoli there was margination of chromatin. particularly striking was an increase in interchromatin granules. changes in the nuclear membrane ... | 1975 | 172041 |
| sodium periodate modification of factor viii procoagulant activity. | chemical treatment of human factor viii by sodium periodate resulted in increased levels of procoagulant activity when measured by the two-stage assay. increased levels of factor-viii activity were observed following periodate treatment of factor-vii concentrates and normal, haemophilic and von willebrand's disease plasmas. however, sodium periodate treatment of haemophilic plasmas which were completely deficient in factor-viii activity did not cause any reduction in the clotting times of two-st ... | 1975 | 172113 |
| on the nature of the iron sulfur cluster in a deuterated algal ferredoxin. | a protonated and a completely deuterated two-iron algal ferredoxin from synechococcus lividus have been studied by optical, electron paramagnetic resonance, electron-nuclear double resonance, proton magnetic resonance and mossbauer spectroscopies; temperature dependent magnetic susceptibility measurements are reported as well. these studies have confirmed the electron localized model of the active center in the two-iron ferredoxins, as previously deduced from studies of spinach ferredoxin, have ... | 1975 | 172131 |
| dependence of gonadotropin-induced steroidogenesis upon rna and protein synthesis in the interstitial cells of the rat testis. | the effects of inhibitors of rna and protein synthesis upon gonadotropic stimulation of testosterone and cyclic amp production by the leydig cellwere investigated in vitro with enzyme-dispersed interstitial cells of the rat testis. the testosterone response to human chorionic gonadotropin was abolished by cycloheximide and puromycin, and was markedly reduced by actinomycin d and cordycepin. during 3-h time studies, cycloheximide caused complete inhibition of subsequent steroid production when a ... | 1975 | 172138 |
| distribution of ferritin-conjugated lectins on sialidase-treated membranes of human erythrocytes. | the labeling of sialidase-treated, human erythrocyte membranes with ferritin-conjugates of four plant lectins, concanavalin a, ricinus communis hemagglutinin, bauhinia purpurea hemagglutinin and arachis hypogoea hemagglutinin, is reported. among these ferritin-conjugated lectins, ferritin-conjugated concanavalin a and ferritin-conjugated r. communis hemagglutinin were found in clusters on the sialidase-treated membranes, whereas ferritin-conjugated b. purpurea hemagglutinin and ferritin-conjugat ... | 1975 | 172150 |
| diseases of the central nervous system. meningitis and encephalitis. | 1975 | 172185 | |
| a comparative study on the heat stability of triosephosphate isomerase in psychrophilic, psychrotrophic, and mesophilic clostridia. | the temperature stability of triosephosphate isomerase (ec 5.3.1.1) in the cell-free extracts of psychrophilic, psychrotrophic, and mesophilic clostridium species has been investigated. even though this enzyme was found to be heat labile in the psychrophilic isolates, no detectable loss in activity was evident when cell-free extracts were heated for 1/2 h at the maximum temperature of growth for the organisms. two organisms, each with a maximum growth temperature of 23 degrees c, showed differen ... | 1975 | 172205 |