Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| differential susceptibility of aeromonads and coliforms to cefsulodin. | volumn 62, no. 6, p. 1885, abstract, line 3: "for 102 of 129 aeromonas isolates" should read "for 102 of 130 aeromonas isolates." page 1887, column 1, lines 16 and 17: "and for 13 of 21 coliform isolates" should read "and for 13 of 94 coliform isolates." [this corrects the article on p. 1885 in vol. 62.]. | 1996 | 16535434 |
| chronic colitis after aeromonas hydrophilia infection. | 1996 | 17429262 | |
| effects of modified atmosphere and vacuum packaging on the growth of spoilage and inoculated pathogenic bacteria on fresh poultry. | fresh chicken breast meats inoculated with yersinia enterocolitica and aeromonas hydrophila were packaged in glass jars either containing different compositions of modified atmospheres (ma) (100% co2; 80% co2/20% n2), or in vacuo or containing air, and were stored at 3 +/- 1 degrees c and 8 +/- 1 degrees c. the changes in gas composition as well as y. enterocolitica, a. hydrophila, total aerobic bacterial, total psychotropic, lactobacilli and enterobacteriaceae counts were determined after 0, 1, ... | 1996 | 9123970 |
| behaviour of aeromonas spp. after animal passage. | 1996 | 9070410 | |
| detección de escherichia coli toxigénica (lt) mediante la reacción en cadena de la polimerasa [the detection of toxigenic escherichia coli (lt) by the polymerase chain reaction]. | in this paper it is described the detection enteroxigenic escherichia coli lt (+). this method is based on the amplification of a dna fragment of 400 pairs of bases by polymerase chain reaction (prc). the oligonucleotides were designed by the authors and the characteristic patterns were observed when the samples were submitted to an electrophoresis in an agarose gel at 2%. the pcr had positive results with the strains of escherichia coli 0:149 k; 88 (lt+) collection and with 20 strains isolated ... | 1996 | 9805043 |
| [aeromonas hydrophila pneumonia associated with a traffic accident. report of a case]. | the case of a patient who was driving a car after getting drunk is presented. his car turned over and he fell into an irrigation canal, and, as a result, he suffered from an incomplete drowning syndrome. he was admitted in the intensive care unit with acute inflammatory pneumonia and a strain of aeromonas hydrophila was isolated in blood. the patient's evolution was favorable. it is the first report on a case like this in our country. | 1996 | 9768270 |
| survey on the distribution of vibrionaceae at the seaport areas in taiwan, 1991-1994. | a monthly survey on the distribution of human-pathogenic vibrionaceae of the seawater from five principal harbors in taiwan was conducted by national quarantine service from july, 1991 to february, 1994. of the total 1,167 vibrionaceae isolates, strains of vibrio alginolyticus (449 strains) were the most frequently isolated, followed by vibrio parahaemolyticus (262) , aeromonas hydrophila (153), vibrio cholerae non-o1 (86), and vibrio vulnificus (67). none of vibrio cholerae o1 was isolated. the ... | 1996 | 10592802 |
| in-vitro acquisition of iron from the iron---desferrioxamine complex by aeromonas hydrophila. | 1996 | 11866778 | |
| breast feeding may protect children against campylobacter jejuni associated diarrhoea. | to investigate the bacterial etiology of infant diarrhea in karachi, pakistan, 180 rectal swab samples collected from diarrhea patients under 5 years of age and 30 samples from age- and sex-matched healthy children were analyzed. enteropathogenic organisms were isolated from 70 case samples (38.9%). the two most common etiologic agents were campylobacter jejuni (19.4%) and aeromonas hydrophilia (6.1%). salmonella, shigella, and yersina appear to have a limited incidence in this age group. no ... | 1996 | 12346517 |
| the effect of temperature and water quality on antibody response to aeromonas salmonicida in sunshine bass (morone chrysops x morone saxatilis). | the kinetics of the antibody response to aeromonas salmonicida were determined in sunshine bass (hybrid striped bass: female morone chrysops x male morone saxatilis) acclimated to 10, 18, 24, 29 degrees c, and to 0.15 mg 1(-1) un-ionized ammonia and 200 mg 1(-1) nitrate levels. temperature and water quality factors affected the immune response of sunshine bass. temperatures of 10 degrees c and 18 degrees c decreased the magnitude and delayed the time of the antibody response to a. salmonicida. t ... | 1996 | 9157680 |
| the aeromonas metallo-beta-lactamases: genetics, enzymology, and contribution to drug resistance. | aeromonads are environmental microorganisms that can be responsible for both human and animal infections. individual aeromonas strains can produce up to three different, inducible, chromosomally encoded beta-lactamases, including a group 1 molecular class c cephalosporinase, a group 2d molecular class d penicillinase, and a group 3 molecular class b metallo-beta-lactamase, which contribute to beta-lactam resistance in members of this genus. among these enzymes, the metallo-beta-lactamases are cl ... | 1996 | 9158767 |
| overproduction and purification of the aeromonas hydrophila cpha metallo-beta-lactamase expressed in escherichia coli. | the aeromonas hydrophila cpha metallo-beta-lactamase was overexpressed in a soluble secreted form in escherichia coli using a t7 rna polymerase-based expression system, and a simple protocol based on a single cation-exchange chromatographic step was developed, which is suitable for rapid purification of the overexpressed enzyme from e. coli lysates. a yield of up to 30 micrograms of purified enzyme per milliliter of culture was obtained. the purified enzyme preparation showed properties identica ... | 1996 | 9158768 |
| diffusion of carbapenems through the outer membrane of enterobacteriaceae and correlation of their activities with their periplasmic concentrations. | scarce information is available on the real mechanism by which carbapenemes penetrate in enterobacteriaceae, although a considerable amount of evidence suggests that in many species of this family the lack of certain outer membrane proteins is associated with the acquisition of resistance to these antibiotics. the existance of specific pathways for the carbapenems has never been demonstrated, although at times it has been postulated in both wild and mutant strains, on the basis of evident discor ... | 1996 | 9158772 |
| enzyme kinetics and biochemical analysis of imis, the metallo-beta-lactamase from aeromonas sobria 163a. | the metallo-beta-lactamase from aeromonas sobria 163a, imis, was isolated in a two stage purification procedure using protein affinity columns. enzyme kinetics show that imis hydrolyses the carbapenems but displays poor activity against other beta-lactams. imis possesses the narrowest spectrum of activity of the group 3 enzymes that have been analysed. sequencing of the 40 n-terminal amino acids show this region to be identical to that of the cpha metallo-beta-lactamase from aeromonas hydrophila ... | 1996 | 9182099 |
| production of haemolysin and enterotoxin by aeromonas jandaei and aeromonas trota strains after animal passage. | five aeromonas jandaei and 12 aeromonas trota isolates were tested for the production of haemolysin and enterotoxin, and the correlation between these two properties. the majority (10 isolates) of the strains produced beta-haemolysis. the titres of haemolytic activity for both species were 8-64 hu/ml. in the initial ileal loop test, only two (a. trota) of the 17 isolates produced enterotoxin. one each of these 2 a. trota strains was beta-haemolytic and non-haemolytic. the remaining isolates of a ... | 1996 | 9203791 |
| antidiarrhoeal activities of ocimum gratissimum (lamiaceae). | the antidiarrhoeal activities of leaf extracts of ocimum gratissimum were investigated by disc diffusion and tube dilution methods. the extracts were active against aeromonas sobria, escherichia coli, plesiomonas shigelloides, salmonella typhi, and shigella dysenteriae. the leaf extracts were most active against s. dysenteriae and least active against s. typhi. the sensitivity of the organisms measured in terms of zone of inhibition ranged from 8.00 to 19.50 mm. the minimum inhibitory concentrat ... | 1996 | 9203793 |
| [purification and characterization of s-layer protein from aeromonas hydrophila]. | the regular surface protein array (s-layer) present on aeromonas hydrophila j-1 was composed of a single species of protein of apparent molecular weight 51500. this protein was extracted from whole cells by treatment with 0.2 mol/l glycine hydrocholoide (ph4.0). the protein was purified by sephadex g-200 gel filtration chromatography and an ion exchange chromatography on deae-cellulose. amino acid composition analysis showed that the protein contained 36.8% hydrophobic amino acids. but the prote ... | 1996 | 9206273 |
| metabolism of proteins and glycoproteins in tumour bearing mice treated with aeromonas l-asparaginase. | l-asparaginase, isolated in our laboratory, from aeromonas had been found to be antileukaemic. in the present study, changes in the levels of proteins and glycoproteins in leukaemic mice and under treatment with aeromonas l-asparaginase have been compared. levels of protein bound hexose, fucose and sialic acid which were increased during leukaemia attained normal levels when treated with l-asparaginase. the increased blood urea level declined significantly during enzyme therapy. effects of l-asp ... | 1996 | 9219441 |
| bacterial diarrhea in laos, a region where cholera was endemic. | there were cholera outbreaks in lao pdr since 1993. two provinces were epidemic areas of cholera in 1993. however the area of cholera occurrence increased to 7 provinces through 1994. then a bacterial survey had done for the purpose of public health improvement. epec was markedly isolated from the hospital in vientiane. in apparent infection of salmonella and non-01 vibrio cholerae with soldiers stationed in the island in mekong river belong to vientiane were pointed out. the outbreak of diarrhe ... | 1996 | 9253874 |
| measurement of virulence of aeromonads using a suckling mouse model of infection. | virulent and avirulent strains of aeromonas spp. were identified and virulence quantified using an animal model. virulence was measured by determining a 50% lethal dose (ld50) 43 h after oral administration of live bacteria. the ld50 of virulent aeromonas isolates ranged from log10 7.53 (mean) organisms to log10 8.88 (mean). some isolates were avirulent in this model. detection of cytotoxic activity in culture supernatants correlated with virulence (fisher exact test, p = 0.0029). there was no c ... | 1996 | 8908484 |
| an aeromonas salmonicida gene required for the establishment of infection in rainbow trout (oncorhynchus mykiss). | the asob gene of aeromonas salmonicida is located approximately 9 kb downstream of the structural gene (vapa) for the surface layer (a-layer). the nucleotide sequence of asob was determined and found to encode a putative polytopic cytoplasmic membrane protein which exhibited homology to a number of bacterial transport proteins. allele exchange mutagenesis of asob resulted in a mutant (a449-d) which was avirulent when administered by bath immersion. however, when administered by intraperitoneal i ... | 1996 | 8917088 |
| cloning, characterization and expression of the reca gene of aeromonas salmonicida. | the aeromonas salmonicida a449 reca gene has been cloned, sequenced and expressed in vitro. the predicted amino acid sequence of a. salmonicida reca was determined and, when compared to other reca, was found to possess a number of domains identical to those characterized in escherichia coli reca. the a. salmonicida reca was mobilized into an e. coli reca mutant strain and was shown to allow increased survival in the presence of the chemical mutagen mms and after ultraviolet (uv) irradiation. the ... | 1996 | 8917089 |
| a biochemical protocol for the differentiation of current genomospecies of aeromonas. | a data file consisting of 40 biochemical and physiological tests for the differentiation of aeromonads was constructed based upon existing published data from various geographical locations and laboratories. the data file covers all the current genomospecies (or hybridisation groups) of aeromonas and is therefore applicable to bacteriologists from a wide variety of fields. the protocol derived from the data file was adapted for use with a semi-interactive computer identification program. this pr ... | 1996 | 8837366 |
| [gastroenteritis due to aeromonas in pediatrics]. | in recent years the incidence of gastroenteritis due to aeromonas spp has increased significantly. the purpose of this study was to investigate the etiology and clinical manifestations of this disease. | 1996 | 8849096 |
| differentiation of aeromonas genomospecies using random amplified polymorphic dna polymerase chain reaction (rapd-pcr). | random amplified polymorphic dna polymerase chain reaction (rapd-pcr) was used to investigate the differentiation of the genus aeromonas at the genomospecies level. of 20 primers evaluated, six produced profiles which contained multiple bands capable of differentiating the genomospecies. these six primers were also used in rapd-pcr analyses of clinical and environmental isolates of the different genomospecies. in most cases, each strain gave a unique fingerprint, illustrating genetic heterogenei ... | 1996 | 8849642 |
| a live (delta aroa) aeromonas salmonicida vaccine for furunculosis preferentially stimulates t-cell responses relative to b-cell responses in rainbow trout (oncorhynchus mykiss). | we have previously described (l. m. vaughan, p. r. smith, and t. j. foster, infect. immun. 61:2172-2181, 1993) the construction of a kanamycin-resistant aromatic-dependent mutant of aeromonas salmonicida, the causative agent of furunculosis, and its use as a live vaccine. here we describe the construction of an unmarked aroa deletion mutant and examine the nature and magnitude of immune responses in rainbow trout (oncorhynchus mykiss) to this vaccine strain. good proliferative and antibody respo ... | 1996 | 8751940 |
| evaluation of the dna fingerprinting method aflp as an new tool in bacterial taxonomy. | we investigated the usefulness of a novel dna fingerprinting technique, aflp, which is based on the selective amplification of genomic restriction fragments by pcr, to differentiate bacterial strains at the subgeneric level. in totals, 147 bacterial strains were subjected to aflp fingerprinting: 36 xanthomonas strains, including 23 pathovars of xanthomonas axonopodis and six pathovars of xanthomonas vasicola, one strain of stenotrophomonas, 90 genotypically characterized strains comprising all 1 ... | 1996 | 8757752 |
| application of antimicrobial-producing lactic acid bacteria to control pathogens in ready-to-use vegetables. | five psychrotrophic strains of lactic acid bacteria (lactobacillus casei, lact. plantarum and pediococcus spp.) were isolated from 22 samples of commercial salads. these strains were shown to inhibit aeromonas hydrophila, listeria monocytogenes, salmonella typhimurium and staphylococcus aureus on mrs agar, in salads and in juice prepared from vegetable salads. lactobacillus casei impclc34 was most effective in reducing total mesophilic bacteria and the coliform group; aer. hydrophila, salm. typh ... | 1996 | 8760320 |
| antimicrobial susceptibility and plasmid-mediated streptomycin resistance of plesiomonas shigelloides isolated from blue crab. | five plesiomonas shigelloides strains isolated from retail or wild-catch louisiana blue crabs (callinectes sapidus) were examined for resistance to selected antibiotics and presence of plasmids. three agar media, macconkey, salmonella-shigella and a modification of inositol brilliant green bile salt (ibb), were evaluated for isolation of p. shigelloides. of these, ibb was most satisfactory for distinguishing between p. shigelloides and the related species aeromonas hydrophila. isolates were susc ... | 1996 | 8760329 |
| platelet activating factor mediates cardiopulmonary dysfunction during graded bacteremic shock. | to determine whether or not platelet activating factor (paf) is a necessary mediator of cardiovascular dysfunction during graded bacteremia, and to identify paf interactions with eicosanoids and tumor necrosis factor-alpha (tnf-alpha). | 1996 | 8760539 |
| [rapid detection of salmonella with mucap reagent]. | the 4-methylumbelliferyl-caprylate (mucap) was applied for the identification of salmonella, it's specification, sensitity, and availability were reported. 65 strains of standard salmonella, 48 strains of salmonella isolated from foods growing on plates of he, dhl, ss and maconkey agar have been tested with mucap. all of them were identified as mucap positive; whereas, among the non-salmonella tested only pseudamonas spp., aeromonas hydrophilia and plesiomonas shigelloides were shown as positive ... | 1996 | 8762473 |
| species and serovars of enteropathogenic agents associated with acute diarrheal disease in rosario, argentina. | we report the most frequent species and serovars of enteropathogenic organisms in rosario from 1985 to 1993. enteropathogenic escherichia coli was the most prevalent agent affecting 144/570 (25.2%) children; 0111 represented 41.8%, 055: 13.6%, 0119: 12.7%. among enterotoxigenic e. coli (etec) the most frequent were etec-st 0128:h21 and 0153:h45. shigella spp were isolated in 8.8%; s. flexneri: 7%, principally type 2 (59.5%); s. sonnei: 1.6%, and s. dysenteriae type 2: 0.2%. campylobacter spp wer ... | 1996 | 8762632 |
| atypical aeromonas salmonicida isolated from diseased turbot (scophtalmus maximus l.). | atypical aeromonas salmonicida were isolated from 3 outbreaks of disease among farmed turbot (scophthalmus maximus l.) in 3 different farms, 1 from norway (n1) and 2 from denmark (dk1 and dk2). in all 3 cases, the incidence of disease and mortality was high and the main characteristic pathological finding was skin ulcers and septicaemia. the isolated bacteria were subjected to a thorough phenotypic and genotypic examination and comparison in the laboratory. all 3 isolates belonged to a. salmonic ... | 1996 | 8767692 |
| survival potential of aeromonas hydrophila in freshwaters and nutrient-poor waters in comparison with other bacteria. | the survival of a genetically-marked aeromonas hydrophila strain was studied in water microcosms using viable counts. aeromonas hydrophila awwx1 was shown to survive without decline in viable counts for at least 10 d in three of four filtered-autoclaved freshwaters (surface water and groundwater) and in all examined filtered-autoclaved nutrient-poor waters (bottled spring water, milli-q and tap water). however, in the unfiltered waters, a rapid decrease in viable counts of aer. hydrophila awwx1 ... | 1996 | 8852674 |
| pulsed-field gel electrophoresis as an epidemiological tool for clonal identification of aeromonas hydrophila. | pulsed-field gel electrophoresis (pfge) was used to characterize aeromonas hydrophila strains isolated from a cluster of hospital-acquired infections that occurred over approximately 1 month in a french hospital. five isolates from patients and 10 isolates from the water supply were characterized by biotyping and antibiotic susceptibility patterns and compared with 10 epidemiologically unrelated strains isolated from patients and rivers, by pfge of digests of chromosomal dna. five environmental ... | 1996 | 8852675 |
| hlya hemolysin of vibrio cholerae o1 biotype e1 tor. identification of the hemolytic complex and evidence for the formation of anion-selective ion-permeable channels. | hemolysin (hlya) was concentrated from supernatants of different vibrio cholerae o1 biotype e1 tor strains by ammonium sulfate precipitation. the concentration of the toxin in the supernatants and in the precipitates was quantified using its hemolytic activity. the toxin formed a high molecular-mass band (about 220 kda) on sds/page while the toxin monomer had a molecular mass of 60 kda when it was heated. the addition of the e1 tor hemolysin oligomers, but not that of the monomers, to the aqueou ... | 1996 | 8856066 |
| hazard identification in swine slaughter with respect to foodborne bacteria. | swine slaughter is an open process with many opportunities for the contamination of the pork carcass with potentially pathogenic bacteria; however, it does not contain any point where hazards are completely eliminated. data on the prevalence of various pathogenic bacteria (aeromonas hydrophila, campylobacter coli/jejuni, listeria monocytogenes, salmonella spp., staphylococcus aureus and yersinia enterocolitica) in pigs, their growth and survival characteristics and ability to become established ... | 1996 | 8856371 |
| diarrheal and environmental isolates of aeromonas spp. produce a toxin similar to shiga-like toxin 1. | diarrheal and environmental isolates of 39 strains of aeromonas spp. were studied for detection of virulence factors. although these 39 strains did not produce either heat-labile or heat-stable enterotoxins, culture filtrates of 31 strains produced cytopathic effects on vero cells. among these, culture filtrates of three strains of aeromonas hydrophila and one strain of aeromonas caviae could be neutralized by escherichia coli o157:h7 shiga-like toxin 1 antiserum. a single band of plasmid dna of ... | 1996 | 8857271 |
| structural studies of the o-antigenic polysaccharide from an aeromonas caviae strain. | the structure of the o-antigenic polysaccharide from a strain of aeromonas caviae, isolated from the stools of a patient with diarrhoea, has been investigated. sugar analysis, methylation analyses, and a uronic acid degradation together with nmr spectroscopy were the principal methods used. the sequence of the sugar residues could be determined by noesy and hmbc experiments. it is concluded that the polysaccharide is composed of pentasaccharide repeating units with the following structure: [sequ ... | 1996 | 8864229 |
| comparative analysis of the cya locus in enterobacteria and related gram-negative facultative anaerobes. | comparison of the cya loci (cya codes for adenylyl cyclase (ac)) from a variety of phylogenetically divergent facultative anaerobic gram-negative bacteria reveals conserved sequence features. the entire locus structure in enterobacteria is preserved, including two major promoters (a conserved cya strong promoter, p2, and a divergent promoter for a heme biosynthetic operon, hemcd) present in the upstream region of the cya gene. the region between hemc and cya is much longer in proteus mirabilis t ... | 1996 | 8874804 |
| cloning and detection of the hemolysin gene of vibrio anguillarum. | a 5 kb dna fragment encoding a hemolysin was cloned from the fish pathogenic bacterium vibrio anguillarum using the cosmid vector charomid9-36. an open reading frame of the hemolysin gene (vah1) was 2253 bp and corresponded to a protein of 751 amino acid residues. the deduced amino acid sequence of the vah1 gene and the previously reported vibrio cholerae ei tor hemolysin, v. vulnificus cytolysin-hemolysin, aeromonas hydrophila ahh1 hemolysin, and a. salmonicida ash4 hemolysin showed a significa ... | 1996 | 8878014 |
| thermal and biological treatments to control psychrotrophic pathogens. | over the past decade, advances in egg processing technologies have permitted commercial production of ultrapasteurized liquid whole egg (lwe) products with a shelf-life of greater than 10 wk at 4 c. the inactivation and control of psychrotrophic pathogens such as listeria monocytogenes and aeromonas hydrophila in extended shelf-life lwe and conventionally pasteurized egg products is an ongoing food safety concern. this manuscript reports on the common features of these two psychrotrophic pathoge ... | 1996 | 8878273 |
| use of the fluorescent dye picogreen for quantification of pcr products after agarose gel electrophoresis. | 1996 | 8879566 | |
| changes in microbial populations on fresh cut spinach. | the microbial populations found on fresh-cut spinach leaves that were stored in gas permeable bags at 10 degrees c for 12 days were examined and identified. the microorganisms consisted of mesophilic aerobic bacteria, psychrotrophic bacteria, pseudomonadaceae, enterobacteriaceae, micrococcaceae, lactic acid bacteria and yeasts. populations of mesophiles, psychrotrophs, pseudomonadaceae and enterobacteriaceae increased sharply during the storage period. the initial populations were 10(7), 10(6), ... | 1996 | 8880301 |
| incidence and toxigenicity of aeromonas hydrophila in seafood. | three selective media, oxoid aeromonas agar (oa), blood ampicillin agar (ba) and starch ampicillin agar (sa) were used to evaluate the presence of aeromonas hydrophila in 66 samples of oyster, shrimp, fish and surimi products. oyster had the highest incidence, with 50% positive, whilst no a. hydrophila was found in the surimi. of the three selective media, ba displayed the highest recovery rate of a. hydrophila from seafood. forty-eight isolates from this survey were tested for their capability ... | 1996 | 8880302 |
| sparfloxacin worldwide in vitro literature: isolate data available through 1994. | sparfloxacin is a piperazinyl, cyclopropyl-fluoroquinolone with broad-spectrum antibacterial activity. compared to other quinolones, sparfloxacin displays improved activity against a variety of pathogens including staphylococcus, streptococcus, enterococcus, chlamydia, mycoplasma, ureaplasma, and mycobacteria species. other susceptible organism group include haemophilus, legionella, moraxella, neisseria, aeromonas, acinetobacter, bordetella, brucella, campylobacter, gardnerella, and helicobacter ... | 1996 | 8882890 |
| the cardiopulmonary, eicosanoid, and tissue microanatomic effects of fluconazole during graded bacteremia. | imidazole compounds have been shown to be beneficial in systemic sepsis and inflammation. the purpose of this study was to delineate the effects of fluconazole on systemic hemodynamics and on microanatomy of the heart, lung, liver, and kidney parenchyma of swine during graded bacteremia. eighteen adult swine were studied in three groups: 1), anesthesia control; 2), septic control (aeromonas hydrophila, 10(9)/ml, infused i.v. for 4 h); 3) fluconazole (fluconazole, 30 mg/kg i.v., followed by a. hy ... | 1996 | 8885087 |
| expression of aeromonas caviae bla genes in escherichia coli. | an isolate of aeromonas caviae 035 carried a 55.5 kb self-transferable plasmid. transfer of the plasmid to escherichia coli k12 resulted in the expression of a tem-like beta-lactamase that was not expressed in parental a. caviae. the bla gene sequence was detectable by dna hybridization and pcr amplification of the plasmid when extracted from parental a. caviae or from e. coli k12 transconjugants. sequence analysis of the bla genes revealed in each case an 858 bp open reading frame, differing fr ... | 1996 | 8889718 |
| holy water--a risk factor for hospital-acquired infection. | a case of hospital-acquired infection due to acinetobacter baumanii in a burns patient after exposure to holy water is described. in order to assess the infection risk, 13 samples of holy water were cultured for bacteria, (including legionellae) and yeasts. viable bacterial counts ranged from 1 center dot 3 x 10(3)-3 center dot 8 x 10(8) cfu/l (mean 3 center dot 1 x 10(7) cfu/l). a wide range of bacterial species was isolated including pseudomonas aeruginosa, enterobacter spp. escherichia coli a ... | 1996 | 8904372 |
| aetiological role of aeromonas and plesiomonas species in human diarrhoeal illness in nigeria. | 1996 | 8771938 | |
| a fresh water pathogen and its pedal manifestations. | 1996 | 8775178 | |
| production of extracellular proteases and amylases by some acidophilic and alkalophilic bacteria. | twelve bacteria were isolated from two effluent sources of shaw-wallace gelatins, jabalpur. six bacteria from dicalcium phosphate plant effluent (ph-5) and six from main drain of the factory (ph-10) were isolated. two facultatively acidophilic and two facultatively alkalophilic bacteria were selected and tentatively identified as plesiomonas shigelloides, aeromonas hydrophilla, klebsiella pneumoniae and staphylococcus saprophyticus respectively. acidic amylases were produced in higher amounts on ... | 1996 | 8781033 |
| expression of the chitinase iii gene of aeromonas sp. no. 10s-24 in escherichia coli. | the chitinase iii gene of aeromonas sp. no. 10s-24 was expressed in escherichia coli. production of chitinase iii by e. coli was 3-fold higher than that of chitinases in the culture supernatant of aeromonas sp. the enzyme from e. coli was purified and characterized. the molecular weight of the chitinase iii from e. coli was estimated by sds-polyacrylamide gel electrophoresis (sds-page) to be 55,000. this agreed with the calculated molecular weight of the mature chitinase iii (54,111). however, i ... | 1996 | 8782417 |
| evaluation of chromagar orientation for differentiation and presumptive identification of gram-negative bacilli and enterococcus species. | a new chromogenic plate medium, chromagar orientation, was evaluated for use in the differentiation and presumptive identification of gram-negative bacilli and enterococcus species by a multipoint inoculation (replicator) technique. in this study, 1,404 gram-negative bacilli and 74 enterococcal isolates were tested on chromagar orientation. six control american type culture collection strains were also included with the testing to ensure quality control of the media. of the escherichia coli isol ... | 1996 | 8784591 |
| differential susceptibility of aeromonads and coliforms to cefsulodin. | cefsulodin was evaluated as a potential selective agent for aeromonads. resistance of aeromonas and coliform isolates was determined by using a standard disk diffusion technique. a total of 119 aeromonas and 78 coliform strains were isolated. for 102 of 130 [corrected] aeromonas isolates (environmental and reference strains), the mic of cefsulodin was < 8 micrograms/ml. results of mic tests by the agar dilution method showed that a concentration of cefsulodin of 10 micrograms/ml or less inhibite ... | 1996 | 8787387 |
| optical single-channel analysis of the aerolysin pore in erythrocyte membranes. | scanning microphotolysis (scamp), a recently developed photobleaching technique, was used to analyze the transport of two small organic anions and one inorganic cation through single pores formed in human erythrocyte membranes by the channel-forming toxin aerolysin secreted by aeromonas species. the transport rate constants of erythrocyte ghosts carrying a single aerolysin pore were determined to be (1.83 +/- 0.43) x 10(-3) s-1 for lucifer yellow, (0.33 +/- 0.10) x 10(-3) s-1 for carboxyfluoresc ... | 1996 | 8789089 |
| prevention of suicide phenomenon in aeromonads. | clinical isolates of aeromonas (13 aeromonas caviae), 8 aeromonas hydrophila, 3 aeromonas spp., and 2 aeromonas media recovered from diarrheal feces of children were submitted to the suicide phenomenon test and investigated at intervals of 24 h for up to 120 h. the same isolates were also stimulated by repeated passages in broth for ten days before the test. after the bacterial stimulus, a decreases in the number of aeromonads with suicidal capacity was observed. the suicide phenomenon was expre ... | 1996 | 8793406 |
| inactivation of aeromonas hydrophila by fe(ii)-related-radical generation in oxidizing groundwaters. | the survival of aeromonas hydrophila awwx1 in filter-sterilized phreatic groundwaters was studied by using viable counts. aeromonas counts rapidly decreased 2 to 3 log units in oxidizing raw groundwaters from snellegem and beernem, belgium (snellegem-raw and beernem-raw, respectively), containing high concentrations of fe2+ (460 to 1,070 microm). the rapid decline in viable counts of aeromonas cells in the oxidizing raw groundwater of snellegem was prevented by the addition of an fe2+ chelator ( ... | 1996 | 8795217 |
| aeromonas hydrophila isolated from food and drinking water: hemagglutination, hemolysis, and cytotoxicity for a human intestinal cell line (ht-29). | aeromonas hydrophila isolated from food and drinking water was tested for pathogenicity by studying its hemolysis, hemagglutination, and cytotoxicity. hemolysis, tested on erythrocytes from six different species, was more frequently seen with water isolates (64%) than with food isolates (48%). hemagglutination was more frequently encountered with food isolates (92%) than with water isolates (73%). cytotoxicity, evaluated on seven cell lines, was frequently observed with food isolates (92%) and w ... | 1996 | 8795237 |
| differential media for quantitative recovery of waterborne aeromonas hydrophila. | because of the ubiquity of aeromonas spp., their prevalence in drinking water, and the increasing number of reports on aeromonas sp.-related infections, a standard method for routine and quantitative recovery had to be defined. on the basis of a survey of 10 media for recovery analysis and subsequent differentiation assays in mixed cultures, we conclude that ampicillin-dextrin agar performed the best for the recovery of aeromonas spp. in drinking water and the differentiation by simple criteria ... | 1996 | 8795251 |
| fulminant pneumonia and sepsis due to aeromonas hydrophila in an alcohol abuser. | a 69-year-old alcoholic man with pneumonia and sepsis due to aeromonas hydrophila is presented. he died of suffocation by a copious amount of hemoptysis six hours after his first symptoms of abdominal pain, diarrhea and dyspnea. aeromonas hydrophila was isolated from blood and bronchial secretion. a fulminant form of pneumonia could develop in patients with predisposing underlying conditions such as alcoholism with chronic hepatitis and diabetes mellitus. aeromonas hydrophila pneumonia may be ch ... | 1996 | 8797058 |
| multiple regulation of carp (cyprinus carpio l.) macrophages and neutrophilic granulocytes by serum factors: influence of infection with atypical aeromonas salmonicida. | normal carp serum contains inhibitory and stimulatory factors for macrophage and neutrophilic granulocyte respiratory burst activity. as stimulatory factors were only effective in combination with phorbol myristate actetate (pma) activation, it is concluded that they are probably linked to protein kinase c activation. both the stimulatory and inhibitory factors are heat stable. macrophage- and neutrophilic granulocyte-enriched cell fractions from the pronephros of carp had high respiratory burst ... | 1996 | 8797288 |
| characterization and ion channel activities of novel antibacterial proteins from the skin mucosa of carp (cyprinus carpio). | a detergent-solubilized fraction of skin mucus of carp (cyprinus carpio) induced ion channels after reconstitution into planar lipid bilayers. a differential extraction using a non-ionic detergent followed by electrophoretic separation led to the isolation of two hydrophobic 31-kda and 27-kda proteins. in contrast to the 27-kda protein, which was glycosylated, the 31-kda did not bind to concanavalin a. the reconstitution of these proteins into a planar lipid bilayer restored the ionophore behavi ... | 1996 | 8797847 |
| ecological relationship between aeromonas and vibrio spp. and planktonic copepods in the coastal marine environment in southern italy. | the colonisation of planktonic copepod integument by bacteria belonging to the family of vibrionaceae is a well described phenomenon. in this study, besides reporting on the occurrence of vibrionaceae and other enteropathogens, we further report on the bacterial attachment to the estuarine copepod acartia margalefi in a faecal polluted coastal lagoon near naples, southern italy. in addition, we also performed a laboratory experiment to study the ability of 7 bacterial strains (vibrio cholerae no ... | 1996 | 8800550 |
| survival of cells and dna of aeromonas salmonicida released into aquatic microcosms. | the survival of the bacterial fish pathogen aeromonas salmonicida, and persistence of its dna, were monitored in aquatic microcosms using selective culture and most probable number pcr. bacterial cells and naked dna were released into natural non-sterile microcosms consisting of lake sediment overlayered with lake water. two different types of surface sediment were used. one was sandy in character, taken from the shoreline whilst the other was a littoral loamy surface mud. inoculated cells and n ... | 1996 | 8810058 |
| the role of the capsular polysaccharide of aeromonas salmonicida in the adherence and invasion of fish cell lines. | the ability of several aeromonas salmonicida strains grown under different conditions (capsulated and non-capsulated) to adhere to and invade two fish cell lines was compared. the level of adherence was slightly higher when the strains were grown under conditions promoting capsule formation than when the same strains were grown under conditions which did not promote capsule formation. however, the most significant difference among the wild-type strains grown under conditions promoting capsule fo ... | 1996 | 8810501 |
| differentiation of thermolysins and serralysins by monoclonal antibodies. | two monoclonal antibodies (mabs) to a 36-kda extracellular metalloprotease (pscp) from burkholderia (pseudomonas) cepacia were found to react with thermolysin, pseudomonas aeruginosa elastase, alkaline protease (apr) and lasa, serratia marcescens protease (smp), aeromonas hydrophila protease (ahp), and both the lethal factor (lf) and protective antigen (pa) of bacillus anthracis on immunoblots. the mabs were capable of neutralising the proteolytic activity of thermolysin, p. aeruginosa elastase ... | 1996 | 8810950 |
| association of aeromonas hydrophila and vibrio alginolyticus with larval mortalities of scallop (argopecten purpuratus) | a bacteriological study was carried out in a hatchery of argopecten purpuratus located in northern chile which had been affected by severe larval mortalities. the phenotypic characterization of the bacterial strains revealed that vibrio alginolyticus was the predominant species isolated in the majority of samples taken from the different units of the hatchery (microalgae, swimming larvae, seawater of larval culture tanks, and a reservoir tank of 50-μm filtered seawater). however, the bacterial p ... | 1996 | 8812601 |
| further studies on biochemical characteristics and serologic properties of the genus aeromonas. | we characterized a collection of 268 aeromonas isolates from diverse sources (clinical, animal, and environmental sources) for their species and serogroup designations. overall, 97% of these strains could be identified to the genomospecies level by using an expanded battery of biochemical tests. members of the aeromonas hydrophila complex (a. hydrophila, hg2, and a. salmonicida), a group that has previously been difficult to separate biochemically, could easily be distinguished from one another ... | 1996 | 8818884 |
| cloning of an aeromonas hydrophila type iv pilus biogenesis gene cluster: complementation of pilus assembly functions and characterization of a type iv leader peptidase/n-methyltransferase required for extracellular protein secretion. | aeromonas hydrophila secretes several extracellular proteins that are associated with virulence including an enterotoxin, a protease, and the hole-forming toxin, aerolysin. these degradative enzymes and toxins are exported by a conserved pathway found in many gram-negative bacteria. in pseudomonas aeruginosa this export pathway and type iv pilus biogenesis are dependent on the product of the pild gene. pild is a bifunctional enzyme that processes components of the extracellular secretory pathway ... | 1996 | 8820654 |
| [bacteriological studies of travellar's diarrhoea. 5) analysis of enteropathogenic bacteria at osaka airport quarantine station from january 1992 through september 3rd, 1994]. | during the last 2 years and 8 months before the closure of osaka airport quarantine station (from jan. 1992 to sep. 3, 1994), a total of 7,421,909 overseas travellers were quarantined. 15,919 reported themselves of suffering from diarrhoea. bacteriological examination of a total of 6,031 individuals' stools were performed. 1) various enteropathogenic bacteria were isolated from 31.2% of the stools examined. isolated species were as follows: plesiomonas shigelloides, 1,127 cases (59.9%); vibrio p ... | 1996 | 8822051 |
| [in vitro antibacterial activity of pazufloxacin (pzfx) against clinical isolates from infectious enteritis]. | antibacterial activity of pazufloxacin (pzfx) against 65 strains of shigella spp., 13 strains of salmonella spp., 7 strains of escherichia coli, 4 strains of vibrio parahaemolyticus, 4 strains of v. cholerae o1, 2 strains of aeromonas spp., 4 strains of plesionomas shigelloides and 3 strains of v. cholerae non-o1 isolated from patients of infectious enteritis and carriers was compared with that of ofloxacin (oflx), ciprofloxacin (cpfx), tosufloxacin (tflx) and nalidixic acid (na). the mic90 of p ... | 1996 | 8822053 |
| synergistic iron reduction and citrate dissimilation by shewanella alga and aeromonas veronii | two bacterial isolates from great bay estuary, new hampshire, in co-culture carried out anaerobic dissimilation of citric acid with fe(iii) as the terminal electron acceptor. neither isolate oxidized citrate with fe(iii) anaerobically in axenic culture. the fe(iii) reducer, shewanella alga strain bry, did not grow anaerobically with citrate as an energy source. the citrate utilizer, aeromonas veronii, did not reduce iron axenically with a variety of electron donors including citrate. the onset o ... | 1996 | 8824150 |
| aerolysin--the ins and outs of a model channel-forming toxin. | aerolysin is one of a large group of bacterial proteins that can kill target cells by forming discrete channels in their plasma membranes. the toxin has many properties in common with the porins of the gram-negative bacterial outer membrane, including an extensive amount of beta-structure, a high proportion of hydrophilic amino acid side-chains and no hydrophobic stretches in the primary structure. it also oligomerizes to produce an insertion-competent state. aerolysin is secreted as a dimer by ... | 1996 | 8825766 |
| characterization of a type iv bundle-forming pilus (sfp) from a gastroenteritis-associated strain of aeromonas veronii biovar sobria. | the colonization mechanisms of enteropathogenic aeromonas strains are poorly characterized, but recent studies indicate that some filamentous structures are intestinal adhesins. this study describes the purification and characterization of a long, flexible pilus from a gastroenteritis-associated strain of aeromonas veronii biovar sobria. sds-page analysis (various conditions) of pili preparations yielded a pilin protein band of approximately 21 kda. its n-terminal amino acid sequence was unambig ... | 1996 | 8827704 |
| application of a modified disc diffusion technique to antimicrobial susceptibility testing of vibrio anguillarum and aeromonas salmonicida clinical isolates. | two techniques for antimicrobial susceptibility testing of vibrio anguillarum and aeromonas salmonicida strains were compared. the first method was the reference test that determines minimal inhibitory concentrations (mic); the second was a modified diffusion test that measures the inhibitory concentrations in diffusion (icd) by carrying out the diffusion test with five discs of differing contents. icd measurement was not applicable for the susceptibility testing of oxytetracycline and sulfadime ... | 1996 | 8828130 |
| gram negative bacteria and contact lens induced acute red eye. | two patients using hydrogel contact lenses on a daily wear schedule slept overnight with the lenses and woke up with a contact lens induced acute red eye (clare). the contact lenses recovered aseptically at the time of the event grew significant colonies of pseudomonas aeruginosa and aeromonas hydrophila in patient a and pseudomonas aeruginosa and serratia liquefaciens from patient b. similar organisms from the contact lenses were recovered from the lens case and lens care solutions of patient b ... | 1996 | 8828303 |
| prolyl aminopeptidase is also present in enterobacteriaceae: cloning and sequencing of the hafnia alvei enzyme-gene and characterization of the expressed enzyme. | the hafnia alvei prolyl aminopeptidase gene (hpap) was cloned and sequenced, the expressed enzyme (hpap) was purified to homogeneity and thoroughly characterized. an open reading frame of 1,281 bp was found to code for the enzyme, resulting in a protein of 427 amino acids with a molecular weight of 48,577. hpap resembles the aeromonas sobria enzyme, having 45% identity and the same distinctive properties with respect to size and substrate specificities. both enzyme show similar chromatographic b ... | 1996 | 8830041 |
| the role of the o-antigen lipopolysaccharide on the colonization in vivo of the germfree chicken gut by aeromonas hydrophila serogroup o:34. | we compared the ability of different aeromonas hydrophila strains from serogroup o:34 grown at different temperatures to colonize in vivo the germfree chicken gut. we found a good colonization when the strains were grown at 20 degrees c but not when they were grown at 37 degrees c. we previously described that these strains were able to form the o-antigen lipopolysaccharide (lps) when they grow at low temperature but not at high temperature. we also obtained by transposon mutagenesis mutants onl ... | 1996 | 8831828 |
| using a new inbred fish model and cultured fish tissue cells to study aeromonas hydrophila and yersinia ruckeri pathogenesis. | an inbred strain of the southern platyfish, xiphophorus maculatus, was used as a host for aeromonas hydrophila and yersinia ruckeri infections. the infections were initiated by holding the platyfish in inoculation baths containing dilutions of virulent a. hydrophila or y. ruckeri strains. inoculating the platyfish in this manner resulted in a dose-dependent mortality over a range of bacterial input from 10(5) to 10(8) a. hydrophila and 10(6) to 10(8) y. ruckeri/ml. clinical manifestations of a. ... | 1996 | 8722100 |
| prevalence and serovar distribution of salmonella in fresh and frozen meat from captive nile crocodiles (crocodylus niloticus). | salmonella was isolated from 6 of 20 (30.0%) samples of fresh meat, and from 28 of 140 (20.0%) samples of frozen meat processed for human consumption from captive nile crocodiles (crocodylus niloticus) in zimbabwe. salmonella enterica isolates showed a serovar distribution of 41.2% (14/34) subsp. enterica, 11.8% (4/34) subsp. salamae and 41.2% (14/34) subsp. diarizonae. analyses of fresh meat samples yielded aeromonas (a. hydrophila) in 18 of 20 samples (90%), and a mean aerobic plate count (apc ... | 1996 | 8722192 |
| development of aeromonas hydrophila bacteremia in a patient recovering from cholera. | 1996 | 8722957 | |
| cloning and nucleotide sequence of the dna gyrase gyra gene from the fish pathogen aeromonas salmonicida. | the dna gyrase gyra gene from the fish pathogen aeromonas salmonicida 2148/89 was cloned, and the nucleotide sequence was determined. an open reading frame of 2,766 nucleotides was identified and was found to encode a protein of 922 amino acids with a calculated molecular mass of 101.1 kda. the derived amino acid sequence shared a high degree of identity with other dna gyrase a proteins, in particular, with other gram-negative gyra sequences. when the amino acid sequence of a. salmonicida gyra w ... | 1996 | 8723452 |
| increasing antibiotic resistance in clinical isolates of aeromonas strains in taiwan. | a total of 234 clinical isolates of aeromonas, primarily a. hydrophila, were collected for the present study. most were isolates from blood. by the agar dilution method, more than 90% of the aeromonas strains were found to be susceptible to moxalactam, ceftazidime, cefepime, aztreonam, imipenem, amikacin, and fluoroquinolones, but they were more resistant to tetracycline, trimethoprim-sulfamethoxazole, some extended-spectrum cephalosporins, and aminoglycosides than strains from the united states ... | 1996 | 8723478 |
| exploring the use of the medicinal leech: a clinical risk-benefit analysis. | the medicinal leech, hirudo medicinalis, has been used with increasing frequency for salvage of compromised pedicled flaps and microvascular free-tissue transfers. the data pertaining to 18 cases in which flap salvage with leeches was attempted were reviewed and contrasted with the data from 108 published cases from other centers. a further analysis isolated and examined 19 cases in which infection, secondary to leech utilization, supervened. results suggested that successful salvage of tissue w ... | 1996 | 8726336 |
| bacterial aminopeptidases: properties and functions. | aminopeptidases are exopeptidases that selectively release n-terminal amino acid residues from polypeptides and proteins. bacteria display several aminopeptidasic activities which may be localised in the cytoplasm, on membranes, associated with the cell envelope or secreted into the extracellular media. studies on the bacterial aminopeptide system have been carried out over the past three decades and are significant in fundamental and biotechnological domains. at present, about one hundred bacte ... | 1996 | 8703509 |
| utilization of transferrin and salmon serum as sources of iron by typical and atypical strains of aeromonas salmonicida. | the ability of typical and atypical strains of aeromonas salmonicida to utilize non-haem sources of protein-bound iron was evaluated. (i) in a plate bioassay, the suppression of growth imposed on typical and atypical a. salmonicida by addition of the high-affinity iron chelator ethylenediamine-di(o- hydroxyphenylacetic acid) (edda) to the growth medium was reversed by the addition of 30% or 90% iron-saturated bovine or human transferrin (tf) or lactoferrin (lf) to the growth medium. (ii) the mec ... | 1996 | 8704995 |
| microbiological quality & incidence of organisms of public health importance in food & water in ludhiana. | bacteriological analysis of 713 samples of various types of foods and related articles and potable water samples from different places in ludhiana, punjab was carried out. the highest counts ranging from 2.5 x 10(6)-7.5 x 10(8) organisms/g were observed in raw vegetables and fruits, followed by 3 x 10(6)-9.8 x 10(7)/ml, 8.3 x 10(4)-8.9 x 10(7)/g and 1 x 10(3)-6.7 x 10(7)/g in fruit juice, milk and its products, and salty/non milk snacks respectively. fresh chapati, dal, rice, cooked vegetables a ... | 1996 | 8707360 |
| colonization of streptomycin-treated mice by aeromonas species. | streptomycin-treated adult mice were investigated as a possible model for studying the enteropathogenicity of aeromonas species. c57bl mice pre-treated with streptomycin (5.0 g/l drinking water, 48 hours) received a single intragastric dose (10(10) bacteria /10.5 ml) of one of six well-characterized, toxin-producing, human diarrhoeal isolates of a. veronii biovar sobria (n = 3) or a. hydrophila (n = 3). their faeces were examined for aeromonas for 10 days post-challenge. all strains colonized th ... | 1996 | 8708329 |
| combined effect of gamma-irradiation and conventional cooking on aeromonas hydrophila in meatball. | irradiation combined with a conventional cooking procedure was applied to meatball and the effects on bacterial load and inoculated aeromonas hydrophila were determined. meatball samples were irradiated by using a 60co source at the dose levels of 0, 0.30, 0.75, 1.50, 2.50 kgy and cold stored at 4 +/- 1 degrees c for 7 days. bacterial load and the count of a. hydrophila decreased when the irradiation dose level increased. a minimum inhibition effect was found at the dose of 0.30 kgy. irradiation ... | 1996 | 8717095 |
| aeromonas bacteraemia in burn patients. | human aeromonas infection is uncommon and is usually associated with immunosuppression, chronic disease or trauma in an aquatic setting. burn injury may induce a state of immunosuppression, making the thermally injured patient a suitable host for aeromonas infection. we reviewed the experience of one burn centre with this pathogen. retrospective examination of blood culture results from 8151 patients admitted between 1959 and 1994 disclosed eight patients with clinically relevant aeromonas hydro ... | 1996 | 8719317 |
| cloning of a cluster of chitinase genes from aeromonas sp. no. 10s-24. | a gene encoding chitinases from aeromonas sp. no. 10s-24 was cloned into escherichia coli dh5 alpha using puc19, and its nucleotides were sequenced. the chitinase gene was clustered in orfs (open reading frame) 1 to 4, in a 8-kb fragment of dna. orf-1 consisted of 1608 bp encoding 535 amino acid residues, and orf-2 consisted of 1425 bp encoding 474 amino acid residues. orf-3 was 1617 bp long and encodes a protein consisting of 538 amino acids. orf-4 encodes 287 amino acids of the n-terminal regi ... | 1996 | 8605248 |
| identification of the catalytic triad of the lipase/acyltransferase from aeromonas hydrophila. | aeromonas hydrophila secretes a lipolytic enzyme that has several properties in common with the mammalian enzyme lecithin-cholesterol acyltransferase. we have recently shown that it is a member of a newly described group of proteins that contain five similar blocks of sequence arranged in the same order in their primary structures (c. upton and j. t. buckley, trends biochem. sci. 233:178-179, 1995). assuming that, like other lipases, these enzymes have a ser-asp-his catalytic triad, we used thes ... | 1996 | 8606184 |
| characterisation of the heptameric pore-forming complex of the aeromonas toxin aerolysin using maldi-tof mass spectrometry. | aerolysin, a virulence factor secreted by aeromonas hydrophila, is representative of a group of beta-sheet toxins that must form stable homooligomers in order to be able to insert into biological membranes and generate channels. electron microscopy and image analysis of two-dimensional membrane crystals had previously revealed a structure with 7-fold symmetry suggesting that aerolysin forms heptameric oligomers [wilmsen et al. (1992) embo j. 11, 2457-2463]. however, this unusual molecularity of ... | 1996 | 8617369 |
| aeromonas salmonicida possesses two genes encoding homologs of the major outer membrane protein, ompa. | two homologs of the outer membrane protein ompa were identified in aeromonas salmonicida by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and amino-terminal sequence analyses. an a. salmonicida genomic dna library was constructed by using lambda gem-11 and recombinant phage carrying both genes ompai and ompaii) selected by immunoscreening. a 5.0-kb bamhi fragment containing the two genes in tandem was subcloned in pbluescript and used for further subcloning and seque ... | 1996 | 8626290 |
| fatal aeromonas hydrophila bacteremia in a hemodialysis patient treated with deferoxamine. | a 49-year-old woman undergoing long-term hemodialysis and treated with deferoxamine (dfo) 1.5 g twice weekly for aluminum bone disease developed fever and bilateral calf pain caused by myonecrosis with gas gangrene. she had a rapidly fatal outcome. the cultures of blood and aspirates from both calf muscles demonstrated aeromonas hydrophila. no obvious entry point could be traced. the in vitro growth of the patient's strain was found to be stimulated by the deferoxamine-iron complex in an iron-de ... | 1996 | 8629637 |
| biochemical characterisation, enteropathogenicity and antimicrobial resistance plasmids of clinical and environmental aeromonas isolates. | one hundred and eight strains of aeromonas from clinical and environmental samples were speciated. seven species were identified, the most prevalent of which was a. hydrophila. experimental studies in an animal model with 36 representative strains of different species revealed that all strains could cause significant fluid accumulation in rabbit ileal loops. of 107 strains showing single or multiple antimicrobial resistance, the highest incidence of resistance was shown for beta-lactam antibioti ... | 1996 | 8636960 |
| behaviour of yersinia enterocolitica and aeromonas hydrophila in skim milk during fermentation by various lactobacilli. | in this study, behaviour of yersinia enterocolitica and aeromonas hydrophila in skim milk during fermentation by various lactobacillus sp. were determined. ph values of the skim milk samples were also examined during fermentation. the amount of produced lactic acid and diacetyl/acetoin productions of the lactobacillus sp. were estimated. antimicrobial effects of the lactobacilli on y. enterocolitica and a. hydrophila were also determined by an agar diffusion method. while y. enterocolitica was n ... | 1996 | 8638435 |
| the species composition of oligotrophic bacterial communities of lake balaton--a numerical analysis. | from among the 565 aerobic and facultatively anaerobic, oligotrophic bacterial isolated obtained from the open water region of the balatonfüred-bay, 58 representative strains were selected for detailed taxonomic-physiological studies and numerical analyses as well as simultaneous comparisons with authentic bacterial strains isolated earlier by langó (1987) from different water habitat of the lake balaton. on the basis of 186 ceded differential diagnostic characteristics involved in these analyse ... | 1996 | 9147724 |
| application of a colorimetric dna-dna hybridization method for identification of aeromonas genomic species, isolated from diarrheal stools. | the colorimetric dna-dna hybridization method for the identification of 18 strains of aeromonas spp. isolated from human stools was used. bacterial isolates were also examined by phenotypic characteristics. on the basis of biochemical tests 13 strains were included in phenogroup a. caviae and 5 strains in a. sobria. identification to the species level was obtained by colorimetric hybridization method. dna-dna similarity values showed that isolates of a. caviae group belong to hybridization group ... | 1996 | 9131804 |