Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| aminergic neurotransmitter and water content changes in rats after transient forebrain ischemia. | we have studied changes of cerebral monoamine metabolism and water content, during recirculation following global transient ischemia (20 min) using the four-vessel occlusion model in rats. levels of monoamines and their metabolites were determined in cortex, striatum, hippocampus, and hypothalamus. water content was evaluated by weight and by the analysis of t1 and t2 relaxation times in 1h-nuclear magnetic resonance. norepinephine levels decreased; 3,4-dihydroxyphenylethylamine, 3,4-dihydroxyph ... | 1986 | 2430056 |
| the origin of cells in the glomerular crescent investigated by the use of monoclonal antibodies. | a study was made of the cells forming the crescents in human crescentic glomerulonephritis. the investigation was performed using a panel of antibodies with immunoperoxidase techniques in formalin fixed, paraffin embedded renal biopsy material. some of the cells of glomerular crescents were found to contain cytokeratin intermediate filaments, as did some of the cells of the normal parietal epithelium of bowman's capsule. leucocytes were also found in crescents, often in the outer part, and their ... | 1986 | 2430880 |
| [use of immunospecific components labelled with beta-lactamase and horseradish peroxidase for immunoenzyme analysis]. | a system for enzyme immunoassay with the use of beta-lactamase as an enzyme marker is described. similar sensitivity of the enzyme immunoassays with the use of beta-lactamase and horse-raddish peroxidase as enzyme markers was shown. the data on decrease of the antigen binding capacity of the antibodies in the conjugates with beta-lactamase are presented. it is suggested that such a decrease may be associated with glutaric dialdehyde conjugation. the use of the schemes for enzyme immunoassay with ... | 1986 | 2434023 |
| assessment of ischemia reperfusion injury in skeletal muscle by macromolecular clearance. | qualitative changes in skeletal muscle injury after ischemia are well known; however, quantitative assessments have not been well documented. we have determined microvascular permeability changes by measuring the clearance of fluorescein-labeled dextran of mw 150,000 (fitc-dextran-150). the cremaster muscle of anesthetized rats was fashioned as a single layer, splayed on a lucite chamber and suffused with bicarbonate buffer solution at 35 degrees c. clearance is the product of suffusion rate tim ... | 1987 | 2438477 |
| antiviral treatment of chronic type b hepatitis. | 1987 | 2441635 | |
| discrimination of hepatocyte-stimulating activity from human recombinant tumor necrosis factor alpha. | the involvement of tumor necrosis factor alpha (tnf alpha) in the regulation of acute-phase protein synthesis is currently under discussion. in this study the effect of human recombinant tnf alpha on the regulation of the 4 acute-phase proteins alpha 2-macroglobulin, albumin, alpha 1-proteinase inhibitor and alpha 1-acute-phase globulin was investigated in rat hepatocyte primary cultures. no changes in synthesis of any of the 4 proteins were observed. however, an acute-phase response similar to ... | 1987 | 2441999 |
| [levels of fraction i and vw antigens in cultures of the plague microbe grown on yeast-casein, yeast-hottinger broth and yeast-sunflower oil cake media]. | the content of fraction 1 and vw-antigens in y. pestis cultures grown in different media (yeast-casein medium, yeast medium with hottinger digest, and yeast medium with sunflower-seed protein) was studied over the course of their growth by means of the antibody neutralization and microprecipitation in agar tests. the media under study were not inferior to the casein sulfuric hydrolysate-based medium used for control in their capacity for ensuring the synthesis of vw-antigens. the maximum accumul ... | 1987 | 2442925 |
| translation framing code and frame-monitoring mechanism as suggested by the analysis of mrna and 16 s rrna nucleotide sequences. | protein coding sequences carry an additional message in the form of a universal three-base periodical pattern (g-non-g-n)n, which is expressed as a strong preference for guanines in the first positions of the codons in mrna and lack of guanines in the second positions. this periodicity appears immediately after the initiation codon and is maintained along the mrna as far as the termination triplet, where it disappears abruptly. known cases of ribosome slippage during translation (leaky frameshif ... | 1987 | 2443708 |
| a new look at uterine muscle contraction. | recent progress in our understanding of uterine smooth muscle contraction is reviewed. we no longer believe that actin-myosin interaction in the myometrium occurs through activation of the thin filament; but it is triggered by calcium-dependent phosphorylation of myosin in the thick filament. calcium is now thought to originate from both extracellular and intracellular sources. calcium can enter the cell through either a voltage- or a hormone-controlled calcium channel. the intracellular source ... | 1987 | 2446500 |
| reaction of tryptophanyl-trna synthetase from beef pancreas with periodate-oxidized atp. | tryptophanyl-trna synthetase from beef pancreas reacts with periodate-oxidized atp according to biphasic kinetics. a rapid phase involves two groups of the protein, presumably lysine side-chains. the slow phase corresponds to the reaction of a larger number of groups. the time-course of the partial losses of the atp-ppi isotopic exchange and of the aminoacylation activities of the enzyme follow the labelling of the two fast-reacting groups. however, the ability of the enzyme to form a bis(trypto ... | 1987 | 2820730 |
| antibodies to polymerized human serum albumin in acute and chronic liver disease. | since antibodies to polyalbumin have been noted to occur in patients with hepatitis or cirrhosis, we investigated sera from 219 patients with a variety of acute and chronic liver diseases with and without hbsag using an elisa. the prevalence of circulating polyalbumin antibodies was shown to be significantly increased over that of healthy controls (6.7%) in patients with autoimmune chronic active hepatitis (67%), acute viral hepatitis (41%) and fulminant hepatic necrosis (38%), but such antibodi ... | 1987 | 2820857 |
| antibodies to polymerized human serum albumin in acute and chronic liver disease. | since antibodies to polyalbumin have been noted to occur in patients with hepatitis or cirrhosis, we investigated sera from 219 patients with a variety of acute and chronic liver diseases with and without hbsag using an elisa. the prevalence of circulating polyalbumin antibodies was shown to be significantly increased over that of healthy controls (6.7%) in patients with autoimmune chronic active hepatitis (67%), acute viral hepatitis (41%) and fulminant hepatic necrosis (38%), but such antibodi ... | 1987 | 2820857 |
| [wiedemann-beckwith syndrome: clinical characteristics, constitutional chromosome abnormalities and tumor incidence]. | the observation of constitutional chromosome abnormalities in a one and half year old boy with wiedemann-beckwith syndrome (wbs) and hepatoblastoma prompted us to review the currently available literature dealing with this subject. in approximately 500 published cases with complete and incomplete forms of wbs, 42 malignant and 9 benign neoplasms have been described. malignant tumors, in particular, mainly consisted of nephroblastomas and adrenocortical carcinomas. the patient presented in this p ... | 1987 | 2821320 |
| evidence for histidyl and carboxy groups at the active site of the human placental na+-h+ exchanger. | the na+-h+ exchanger of the human placental brush-border membrane was inhibited by pretreatment of the membrane vesicles with a histidyl-group-specific reagent, diethyl pyrocarbonate and with a carboxy-group-specific reagent, n-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline. in both cases the inhibition was irreversible and non-competitive in nature. but, if the membrane vesicles were treated with these reagents in the presence of amiloride, cimetidine or clonidine, there was no inhibition. since ... | 1987 | 2822022 |
| evidence for histidyl and carboxy groups at the active site of the human placental na+-h+ exchanger. | the na+-h+ exchanger of the human placental brush-border membrane was inhibited by pretreatment of the membrane vesicles with a histidyl-group-specific reagent, diethyl pyrocarbonate and with a carboxy-group-specific reagent, n-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline. in both cases the inhibition was irreversible and non-competitive in nature. but, if the membrane vesicles were treated with these reagents in the presence of amiloride, cimetidine or clonidine, there was no inhibition. since ... | 1987 | 2822022 |
| studies on the immunoglobulin v kappa locus in human lymphoid cell lines. | dna digests of 16 human lymphoid cell lines were studied in blot hybridization experiments with probes from v kappa genes and their immediate neighborhood as well as with single or low-copy probes from intergenic regions. the patterns were compared with those of placenta dna digests in which the kappa genes are in the germline configuration. the differences of patterns which were detected with the first type of hybridization probes can be attributed to v kappa--j kappa rearrangements or to restr ... | 1987 | 2822060 |
| studies on the immunoglobulin v kappa locus in human lymphoid cell lines. | dna digests of 16 human lymphoid cell lines were studied in blot hybridization experiments with probes from v kappa genes and their immediate neighborhood as well as with single or low-copy probes from intergenic regions. the patterns were compared with those of placenta dna digests in which the kappa genes are in the germline configuration. the differences of patterns which were detected with the first type of hybridization probes can be attributed to v kappa--j kappa rearrangements or to restr ... | 1987 | 2822060 |
| repeated sequences of 3-kb dna fragment of a plasmid from yersinia enterocolitica 09 in plasmids from y. pestis and y. pseudotuberculosis. | 1987 | 2822355 | |
| mobilization of the vwa plasmid of yersinia pestis by f-containing strains of escherichia coli. | 1987 | 2822356 | |
| virological implications of the use of primates in xenotransplantation. | 1987 | 2823433 | |
| virological implications of the use of primates in xenotransplantation. | 1987 | 2823433 | |
| [evaluation of the modulating action of yersinia pestis adenylate cyclase on guinea pig peritoneal leukocytes using chemiluminescence]. | the biological action of y. pestis adenylate cyclase on peritoneal leukocytes of guinea pigs has been studied by means of chemiluminescence. y. pestis adenylate cyclase is supposed to contribute to the "oxidation" explosion of phagocytes in plague. | 1987 | 2823508 |
| [cyclic nucleotides in the dynamic development of shock caused by the murine toxin of yersinia pestis]. | the authors have studied the effect of y. pestis "mouse" toxin (ld50), injected intravenously to rats, on camp and cgmp content in the tissues of different organs (the lungs, liver, heart, spleen, kidneys, small intestine) and in the blood in the course of the development of toxinfection shock. the effect of y. pestis "mouse" toxin on cyclic nucleotide content in the organs of experimental animals is determined by the sum of oppositely directed effects produced by the thermostable and thermolabi ... | 1987 | 2823509 |
| near-infrared spectrophotometric monitoring of haemoglobin and cytochrome a, a3 in situ. | 1987 | 2823549 | |
| near-infrared spectrophotometric monitoring of haemoglobin and cytochrome a, a3 in situ. | 1987 | 2823549 | |
| spontaneously regressive canine cutaneous histiocytoma: a counterpart of human regressing atypical histiocytosis? | 1987 | 2823685 | |
| experimental transmission and pathogenesis of immunodeficiency syndrome in cats. | we describe the identification, experimental transmission, and pathogenesis of a naturally occurring powerfully immunosuppressive isolate of feline leukemia virus (designated here as felv-faids) which induces fatal acquired immunodeficiency syndrome (aids) in 100% (25 of 25) of persistently viremic experimentally infected specific pathogen-free (spf) cats after predictable survival periods ranging from less than 3 months (acute immunodeficiency syndrome) to greater than one year (chronic immunod ... | 1987 | 2823940 |
| experimental transmission and pathogenesis of immunodeficiency syndrome in cats. | we describe the identification, experimental transmission, and pathogenesis of a naturally occurring powerfully immunosuppressive isolate of feline leukemia virus (designated here as felv-faids) which induces fatal acquired immunodeficiency syndrome (aids) in 100% (25 of 25) of persistently viremic experimentally infected specific pathogen-free (spf) cats after predictable survival periods ranging from less than 3 months (acute immunodeficiency syndrome) to greater than one year (chronic immunod ... | 1987 | 2823940 |
| comparative investigation of pmn leucocyte antimicrobial potential in animals of different species susceptibility to the plague agent. | the present work was aimed at a comparative study of oxygen-dependent metabolism (om) and myeloperoxidase (mpo) activities as well as the content of bactericidal cationic proteins (bcp) in polymorphonuclear leucocytes (pmnl) from mammals and birds with different susceptibility to plague (mice, guinea pigs, rats, great and miday gerbils, rabbits, dogs, pigeons and man). the experimental finding of an association between infectious susceptibility and functioning of the major pmnl bactericidal syst ... | 1987 | 2824603 |
| chronic administration of sertraline, a selective serotonin uptake inhibitor, decreased the density of beta-adrenergic receptors in rat frontoparietal cortex. | sertraline, a potent and selective inhibitor of serotonin uptake, was chronically administered to laboratory rats. using in vitro receptor autoradiographic techniques, we found that the binding of [3h]dihydroalprenolol ([3h]dha) was reduced in cortex layers iv-vi. results of a saturation experiment indicated that the reduction in cortex layer iv was due to a change in number but not affinity of beta-adrenergic receptors. | 1987 | 2825912 |
| chronic administration of sertraline, a selective serotonin uptake inhibitor, decreased the density of beta-adrenergic receptors in rat frontoparietal cortex. | sertraline, a potent and selective inhibitor of serotonin uptake, was chronically administered to laboratory rats. using in vitro receptor autoradiographic techniques, we found that the binding of [3h]dihydroalprenolol ([3h]dha) was reduced in cortex layers iv-vi. results of a saturation experiment indicated that the reduction in cortex layer iv was due to a change in number but not affinity of beta-adrenergic receptors. | 1987 | 2825912 |
| effects of caffeine and cyclic adenosine 3',5'-monophosphate on adenosine triphosphate-dependent calcium uptake by lysed brain synaptosomes. | the adenosine triphosphate-dependent calcium uptake by endoplasmic reticulum elements of lysed synaptosomes from rat brain cortex was studied. caffeine exhibited a biphasic effect on this calcium uptake activity: concentrations of 1, 2, 5, 10 or 30 mm caffeine stimulated calcium uptake by 62, 111, 73, 88 and 60% respectively, whereas calcium uptake was inhibited by 55% at a 60-mm concentration of caffeine. calcium release from endoplasmic reticulum elements of lysed brain synaptosomes was stimul ... | 1987 | 2825917 |
| protein kinase c inhibitors eliminate hippocampal long-term potentiation. | recent findings suggest that protein kinase c (pkc) regulates the persistence of long-term potentiation (ltp). to test the hypothesis that pkc inhibition would decrease persistence of potentiation we applied pkc inhibitors (mellitin, polymyxin b, h-7) by micropressure ejection to the intact hippocampus either before or after ltp induction. when inhibitor was given 15 min before ltp, initial potentiation was unaffected, yet responses decayed to baseline levels by 60 min after the onset of potenti ... | 1987 | 2825923 |
| agonist-dependent phosphorylation of the alpha 2-adrenergic receptor by the beta-adrenergic receptor kinase. | desensitization of the beta-adrenergic receptor, a receptor which is coupled to the stimulation of adenylate cyclase, may be regulated via phosphorylation by a unique protein kinase. this recently discovered enzyme, known as the beta-adrenergic receptor kinase, only phosphorylates the agonist-occupied form of the beta-adrenergic receptor. to assess whether receptors coupled to the inhibition of adenylate cyclase might also be substrates, we examined the effects of beta-adrenergic receptor kinase ... | 1987 | 2826414 |
| mucosal antibody response of calves after oral and intrabronchial administration of rotavirus. | 1987 | 2827448 | |
| [incompatibility of the cad plasmid from yersinia pestis with plasmids of the incfi group]. | the relation of yersinia pestis calcium dependence plasmid (pcad) to known inc fi (f'lac, r386, pox38) and incfv (f0lac) plasmids has been studied. evidence that plasmid pcad of yersinia pestis belongs to fi incompatibility group is presented. | 1987 | 2828935 |
| galanin-immunoreactive neurons in the guinea-pig small intestine: their projections and relationships to other enteric neurons. | galanin immunoreactivity was observed in nerve cell bodies and nerve fibres, but not in enteroendocrine cells, in the small intestine of the guinea-pig. nerve terminals were found in the myenteric plexus, in the circular muscle, in submucous ganglia, around submucous arterioles, and in the mucosa. lesion studies showed that all terminals were intrinsic to the intestine; those in myenteric ganglia arose from cell bodies in more orally placed ganglia. myenteric nerve cells were also the source of ... | 1987 | 2446770 |
| rapid staining of campylobacter pyloridis. | 1987 | 2447004 | |
| the golgi methods applied to the human and rat subfornical organ. | 1987 | 2448966 | |
| cytomegalovirus infection in fifty-two renal transplant recipients. | 1987 | 2856277 | |
| proteolysis of v antigen from yersinia pestis. | lcr-plasmids of yersiniae are known to mediate a unique low calcium response characterised by restriction of growth in vitro with induction of putative virulence factors including yersiniae outer membrane-peptides (yops) and v antigen (lcr+). a medium was developed that permitted expression of high yields of v by yersinia pestis kim in large fermenter vessels. immunoblots of specific precipitates prepared by prior molecular sieving showed that native unaggregated v exists as a monomeric 37,000 d ... | 1987 | 2853272 |
| enhancement of contractile responses to partial alpha-adrenoceptor agonists during warming in rat aorta. | we examined the effects of warming on the contractile responses to full and partial alpha-adrenoceptor agonists in rat aorta. the contractions elicited by norepinephrine and methoxamine were not affected during warming (40 degrees c, 42 degrees c), whereas those induced by clonidine and st 587 were significantly enhanced. kcl-induced contractions of rat aorta were not affected by warming. the dissociation constants of clonidine and st 587 at 40 degrees c were not different from those at 37 degre ... | 1987 | 2895427 |
| development of aids, hiv seroconversion, and potential co-factors for t4 cell loss in a cohort of intravenous drug users. | a cohort of 334 intravenous (iv) drug users from new york city drug treatment programs were followed over a mean 9-month period. among the 165 who were seropositive at enlistment, four developed clinical aids, for an annual rate of 3%. elevated iga was a significant predictor of developing aids. among 72 subjects who were initially seronegative and who were re-interviewed, four were seropositive at follow-up, for a seroconversion rate of 7% per year among seronegatives. among seropositive subjec ... | 1987 | 2896511 |
| human papillomavirus dna in bronchial squamous cell carcinomas. | 1987 | 2880009 | |
| avaii polymorphism in the human ldl receptor gene. | 1987 | 2881256 | |
| region-specific expression of two mouse homeo box genes. | mammalian homeo box genes have been identified on the basis of sequence homology to drosophila homeotic and segmentation genes. these studies examine the distribution of transcripts from two mouse homeo box genes, hox-2.1 and hox-3.1, throughout the latter third of prenatal development. transcripts from these genes are regionally localized along the rostro-caudal axis of the developing central nervous system, yielding expression patterns very similar to patterns of drosophila homeotic gene expre ... | 1987 | 2881353 |
| glutathione metabolizing enzyme activities in human thyroid. | glutathione peroxidases, glutathione transferase, glutathione reductase and gamma-glutamyl transpeptidase activities were analyzed in human thyroid tissues obtained from 17 patients undergoing resectional surgery because of a malignancy. it was deduced, from measurements of glutathione peroxidase activity with both h202 and cumene hydroperoxide, that thyroid contains only the selenium enzyme. the absence of selenium independent glutathione peroxidase activity in thyroid was confirmed with gel fi ... | 1987 | 2883074 |
| false negative result by the wellcozyme anti-hiv assay in testing an hiv positive haemophiliac. | 1987 | 2883508 | |
| [integrative nature of yersinia pestis virulence]. | 1987 | 2883791 | |
| polyester film as an advantageous tool for light and ultrastructural examination of single cells infected with viruses. | the use of marked transparent polyester film as a support of cell monolayer, smears or sections (cryostat or paraffin), makes possible to examine the same cell preselected in the light microscope by electron microscope (em). consequently, this technique enables to use histological or immunohistological investigations (using immunofluorescence or peroxidase (px) labelling) by light microscopy before examination in em. as example we used herpes simplex virus (hsv) type 1-infected cells grown on th ... | 1987 | 2883863 |
| a new subtype ("brezová") of tribec orbivirus (kemerovo group) isolated from ixodes ricinus males in czechoslovakia. | 1987 | 2883865 | |
| novel approaches towards characterization of the high-affinity nucleotide binding sites on mitochondrial f1-atpase by the fluorescence probes 3'-o-(1-naphthoyl)adenosine di- and triphosphate. | the fluorescence properties of 3'-o-(1-naphthoyl)adenosine di- and triphosphates (termed n-adp and n-atp, respectively) were investigated in detail. of special importance for the use of these analogues as environmental probes is their high quantum yield (0.58 in water) and the polarity dependence of shape and wavelength position of the emission spectrum. upon binding of n-adp and n-atp to mitochondrial f1-atpase, the fluorescence intensity is markedly decreased, due to polarity changes and 'grou ... | 1987 | 2883993 |
| cytoplasmic calcium elevation in hippocampal granule cell induced by perforant path stimulation and l-glutamate application. | calcium-dependent fluorescence of a ca2+ indicator (fura-2) loaded in the slice of guinea pig hippocampus was measured by a microscope/video-camera/photometry system. tetanic stimulation of the perforant path (pp) or application of l-glutamate caused increment of the fluorescence from the dendritic and somatic layers of the granule cells in the dentate gyrus. magnitude of the increment depended on the frequency and intensity of the pp-stimulation or on the dose of l-glutamate. 2-aminophosphonova ... | 1987 | 2884013 |
| two distinct mechanisms for regulation of gamma-glutamyl transpeptidase in cultured rat hepatocytes by glucocorticoid-like steroids. | adult rat hepatocytes maintained in primary monolayer culture with defined medium were used to characterise two effects of glucocorticoid-like steroids in regulating gamma-glutamyltranspeptidase (ggt). low concentrations of glucocorticoids alone had little effect on ggt but synergistically enhanced induction of the enzyme by liver tumor-promoting xenobiotics such as 1,1,1-trichloro-2,2-bis-(4-chlorophenyl)-ethane (ddt) and hexachlorocyclohexane. the enhancing effect appears to be mediated by the ... | 1987 | 2885003 |
| [the spasmolytic activity of propiverine and some of its structural analogs]. | the spasmolytic activity of propiverine and its corresponding methyl ether analogue was investigated in the isolated ileum and urinary bladder of the rat. it could be demonstrated that the antispasmodic properties of propiverine were, in contrast to its methyl ether analogue, not only based on a blockade of muscarinic receptors but were also a consequence of nonspecific papaverine-like effects. investigations with three putative metabolites showed that the alcohol derivative o-desalkylpropiverin ... | 1987 | 2885006 |
| falciparum malaria probably acquired from infected skin-cut. | 1987 | 2885675 | |
| polymorphism of photopigments in the squirrel monkey: a sixth phenotype. | we describe here a trichromatic type of squirrel monkey that resembles old world monkeys in having two well-separated photopigments in the red-green part of the spectrum; the cones of this phenotype have peak sensitivities close to 430, 536 and 564 nm. the existence of such animals is predicted by a genetic model that postulates three alleles for a single locus on the x-chromosome of the squirrel monkey. the three alleles correspond to three different photopigments in the red-green spectral rang ... | 1987 | 2888125 |
| on the rate of f1-atpase turnover during atp hydrolysis by the single catalytic site. evidence that hydrolysis with a slow rate of product release does not occur at the alternating active site. | under conditions of molar excess of enzyme, isolated f1-atpase from beef heart mitochondria catalyses atp hydrolysis biphasically. the rate constants for product release are approximately 10(-1) and 10(-4)-10(-3) s-1, respectively. the slow phase of atp hydrolysis is insensitive to edta. [gamma-32p]atp splitting in the slow phase cannot be chased from the enzyme during several catalytic turnovers. it follows from these results that the slow single-site hydrolysis of atp (kcat approximately 10(-4 ... | 1987 | 2888690 |
| genetic study of piliation in escherichia coli: implications for understanding microbe-host interactions at the molecular level. | 1987 | 2892189 | |
| loss of heterozygosity on chromosomes 3, 13, and 17 in small-cell carcinoma and on chromosome 3 in adenocarcinoma of the lung. | by a molecular genetic approach using polymorphic dna markers that detect allelic deletion of specific chromosomal regions, we analyzed for possible loss of chromosomal heterozygosity in five different histological types of lung cancers obtained from 47 patients. in small-cell carcinomas, the incidence of allelic deletions at three different chromosomal loci was extremely high; loss of heterozygosity was detected on chromosomes 3p in 7 of 7 patients (100%), 13q in 10 of 11 patients (91%), and 17 ... | 1987 | 2892196 |
| [the physiological effects of beta-adrenergic receptor stimulant on the rabbit lacrimal gland]. | 1987 | 2894752 | |
| [studies on the hypothalamus-hypophyseal corticoliberin system. iii. effect of long-term administration of corticoliberin on serum levels of acth, tsh and thyroxine in male rats]. | 1987 | 2842125 | |
| the effect of selective electrical stimulation of non-myelinated vagal fibres on heart rate in the rabbit. | the bradycardia evoked by electrical stimulation of the peripheral cut end of the rabbit vagus nerve is mediated by both myelinated and non-myelinated fibres. the purpose of this study was to assess the effects of non-myelinated fibres on heart rate in the rabbit using selective electrical stimulation techniques. in 8 rabbits selective activation of non-myelinated fibres using reversed polarity triangular shaped pulses (10 hz, 20 s), resulted in a slowly developing fall in heart rate of 24.1 +/- ... | 1987 | 2897392 |
| workers' education. | 1987 | 2953594 | |
| workers' education. | 1987 | 2953594 | |
| the effects of selective dopamine receptor agonists and antagonists on body temperature in rats. | body temperature was measured at repeated time intervals following the administration of various dopamine agonists and antagonists. the d-1 and d-2 receptor agonist, apomorphine, produced dose-dependent hypothermia. this effect was inhibited by the d-2 receptor antagonist, spiroperidol. stimulation of d-2 receptor by ly171555 produced dose-dependent hypothermia, which was attenuated by pretreatment with spiroperidol and not altered by the d-1 receptor antagonist sch23390. the d-1 receptor agonis ... | 1987 | 2951265 |
| purification and immunological studies of selenoprotein a of the clostridial glycine reductase complex. | one of the essential catalytic components of the clostridial glycine reductase complex is a selenium-containing protein, selenoprotein a. an improved method for the purification of selenoprotein a has been developed that yields milligram quantities of the protein in a few relatively simple steps. ferredoxin and rubredoxin can be recovered in pure form as by-products of the procedure. the high resolving capabilities of an anion exchange high pressure liquid chromatography step were exploited in t ... | 1987 | 2951374 |
| neurosecretion of human hypothalamic immunoreactive beta-endorphin: in vitro regulation by dopamine. | an in vitro perifusion system was used to investigate immunoreactive beta-endorphin (beta-end-i) release from adult human hypothalami in response to dopamine (da) and the da receptor antagonist haloperidol (hal). administration of a 1 microm pulse of da consistently elicited a mean (+/- se) 88 +/- 9% increase (p less than 0.05, n = 5) in beta-end-i release, whereas 1 microm hal had no effect. administration of 1 microm da during three perifusions in which 1 microm hal was added to the medium fai ... | 1987 | 2951610 |
| partial transformation of mouse fibroblastic and epithelial cell lines with the v-myc oncogene. | to investigate the role of the myc gene in mammalian cell transformation, plasmid constructs containing the v-myc oncogene and a co-selectable g418 resistance marker were introduced into both mouse fibroblasts (nih-3t3) and bladder epithelial cells (bbn3 and bbn7). after transfection or microinjection of dna, no transformed foci could be detected on confluent monolayers but, when the cells were cultured under conditions in which individual cells were allowed to grow and form colonies, morphologi ... | 1987 | 3023119 |
| the insulin receptor tyrosyl kinase phosphorylates holomeric forms of the guanine nucleotide regulatory proteins gi and go. | an affinity purified human insulin receptor preparation was shown to phosphorylate the alpha- and beta-subunits of the guanine nucleotide-regulatory proteins gi and go, derived from bovine brain. the presence of insulin stimulated the rate of their phosphorylation some 2-fold. the presence of gi and go did not affect the degree of autophosphorylation of the beta-subunit of the insulin receptor. under conditions known to cause the dissociation of gi and go into their constituent subunits then pho ... | 1987 | 3028864 |
| the insulin receptor tyrosyl kinase phosphorylates holomeric forms of the guanine nucleotide regulatory proteins gi and go. | an affinity purified human insulin receptor preparation was shown to phosphorylate the alpha- and beta-subunits of the guanine nucleotide-regulatory proteins gi and go, derived from bovine brain. the presence of insulin stimulated the rate of their phosphorylation some 2-fold. the presence of gi and go did not affect the degree of autophosphorylation of the beta-subunit of the insulin receptor. under conditions known to cause the dissociation of gi and go into their constituent subunits then pho ... | 1987 | 3028864 |
| on the fidelity of dna replication: herpes dna polymerase and its associated exonuclease. | procaryotic dna polymerases contain an associated 3'----5' exonuclease activity which provides a proofreading function and contributes substantially to replication fidelity. dna polymerases of the eucaryotic herpes-type viruses contain similar associated exonuclease activities. we have investigated the fidelity of polymerases purified from wild type herpes simplex virus, as well as from mutator and antimutator strains. on synthetic templates, the herpes enzymes show greater relative exonuclease ... | 1987 | 3029700 |
| cdna cloning of human myeloperoxidase: decrease in myeloperoxidase mrna upon induction of hl-60 cells. | myeloperoxidase (mpo), the most abundant neutrophil protein, is a bacteriocidal component of the primary granules and a critical marker in distinguishing acute myelogenous leukemia from acute lymphoid leukemia. a cdna clone for human mpo was isolated by immunologic screening of human hematopoietic lambda gt11 expression vector libraries with specific anti-mpo antibody. the identity of the cdna clone was confirmed by finding that epitope-selected antibody against this clone recognizes purified mp ... | 1987 | 3031662 |
| cdna cloning of human myeloperoxidase: decrease in myeloperoxidase mrna upon induction of hl-60 cells. | myeloperoxidase (mpo), the most abundant neutrophil protein, is a bacteriocidal component of the primary granules and a critical marker in distinguishing acute myelogenous leukemia from acute lymphoid leukemia. a cdna clone for human mpo was isolated by immunologic screening of human hematopoietic lambda gt11 expression vector libraries with specific anti-mpo antibody. the identity of the cdna clone was confirmed by finding that epitope-selected antibody against this clone recognizes purified mp ... | 1987 | 3031662 |
| membrane conductance and potassium permeability of the rat lens. | the membrane potential, electrical impedance and 86rb+ efflux rate constants were measured in the rat lens perifused at 35 degrees c. the membrane conductance was obtained from the difference between the magnitude of the impedance at low (less than 0.1 hz) and high (greater than 100 hz) frequencies. values of the rubidium permeability coefficients (prb) were obtained from the rate constant and potential data. the values for the membrane potential and conductance in control solution (5 mm potassi ... | 1987 | 3031720 |
| membrane conductance and potassium permeability of the rat lens. | the membrane potential, electrical impedance and 86rb+ efflux rate constants were measured in the rat lens perifused at 35 degrees c. the membrane conductance was obtained from the difference between the magnitude of the impedance at low (less than 0.1 hz) and high (greater than 100 hz) frequencies. values of the rubidium permeability coefficients (prb) were obtained from the rate constant and potential data. the values for the membrane potential and conductance in control solution (5 mm potassi ... | 1987 | 3031720 |
| [detection of an inhibitor of the causative agent of plague active against strains of the plague microbe from an homologous focus. the effect of lithium chloride on detecting the inhibitor]. | a nutrient medium containing 0.7 per cent of lithium chloride was tested and yersinia pestis inhibitor was detected in 83.3 per cent of the strains on the growth tufts of 8 indicator strains isolated from a homologous focus. lowering the lithium chloride concentration in the medium up to 0.6 per cent promoted detection of the inhibitor in a larger number of the tested strains (93.3 per cent). but even on this medium none of the indicator strains formed overall growth tufts. the subcultures of th ... | 1987 | 3032085 |
| [effect of cholera enterotoxin on carbohydrate metabolism of the liver and small intestine mucosa in the rabbit]. | changes in carbohydrate metabolism were studied in the isolated intestinal loops of rabbits during secretory diarrhea, induced by cholera enterotoxin. glucose synthesis level in the small intestinal mucosa and liver was measured by isotope technique, using l-alanine as a precursor. intestinal gluconeogenesis, calculated per mg of protein, appeared to be twice higher than in the liver of fasting rabbits. cholera enterotoxin administration enhanced gluconeogenesis in the liver by 60%, as compared ... | 1987 | 3032299 |
| calmodulin in porcine malignant hyperpyrexia. | the anaesthetic complication malignant hyperpyrexia (mh) is due to an elevation of the myoplasmic ca2+ concentration. examination of calmodulin isolated from mh susceptible swine suggests that the disorder in calcium regulation in mh is not due to an abnormality in calmodulin. | 1987 | 3032704 |
| the mechanisms of antiviral immunity induced by a vaccinia virus recombinant expressing herpes simplex virus type 1 glycoprotein d: clearance of local infection. | we have shown that immunization of mice with a vaccinia virus recombinant expressing glycoprotein d of herpes simplex virus (hsv)-1 will induce a variety of l3t4+ t cell responses. these included a hsv-specific delayed-type hypersensitivity response, t cell help for the induction of antiviral antibodies, and the ability to eliminate a challenge dose of hsv from the pinna. this protection against a subcutaneous virus challenge was not mediated by the delayed-type hypersensitivity response because ... | 1987 | 3033075 |
| more exact quantification of interleukin-2 production by addition of anti-tac monoclonal antibody to cultures of stimulated lymphocytes. | human t-cells produce interleukin-2 (il-2) in response to in vitro stimulation with mitogens and antigens. precise measurement of il-2 production in vitro is hampered by binding of il-2 to il-2 receptors on activated t-cells which results in il-2 consumption. in an attempt to prevent il-2 consumption, we supplemented the cultures with anti-tac, a monoclonal antibody that functionally blocks the human lymphocyte receptor for il-2. addition of anti-tac to cultures of mitogen-stimulated peripheral ... | 1987 | 3033083 |
| detection of antigen to herpes simplex virus in cerebrospinal fluid from patients with herpes simplex encephalitis. | cerebrospinal fluid (csf) specimens were obtained from patients with presumed herpes simplex encephalitis who underwent brain biopsy for diagnostic confirmation. coded csf specimens were fixed on nitrocellulose filter paper and probed with a pool of monoclonal antibodies directed against four herpes simplex virus glycoproteins (gb, gc, gd, and ge). herpes simplex virus antigen was detected in 35 of 40 specimens obtained from 26 biopsy-positive patients. in contrast, only three of 25 specimens fr ... | 1987 | 3033092 |
| effects of inhibitors of the cytoplasmic structures and functions on the early phase of infection of cultured cells with simian virus 40. | to obtain information about cytoplasmic structures and functions involving the entry of simian virus 40 virions into cells, we examined whether the inhibitors that affect the functions and/or structure of lysosomes, cell membrane, and cytoskeletons inhibit expression of nuclear t antigen in the sv40-inoculated rat 3y1 and monkey cv-1 cells. chloroquine, methylamine, and butylamine did not inhibit t-antigen expression, suggesting that lysosomal acidification is not required for establishment of i ... | 1987 | 3033894 |
| maturation antigen of the mouse sperm flagellum: ii. origin from holocrine cells of the distal caput epididymis. | during epididymal transit, the mouse sperm flagellum acquires a surface glycoprotein (sma4) from epididymal fluid that functions as a sperm antiagglutinin. to determine the origin of this molecule, testes and epididymides of male mice were sectioned for light microscopy and stained with wheat germ agglutinin (wga)-peroxidase, a probe that has been used previously to examine the biology of sma4. wga reactivity was localized to the cytoplasm in a small population of cells in the distal caput epidi ... | 1987 | 3034104 |
| metabolism of nicotinamide mononucleotide in beef liver. | nicotinamide mononucleotide (nmn) is not only an intermediate for the biosynthesis but also a degradation product of pyridine cofactors in animal tissues. among the animal tissues tested, the highest nmn catabolizing activity was detected in beef liver (5.6 mumol/min/g tissue). this activity was 16 times higher than the nad hydrolysis catalyzed by the liver nad glycohydrolase. as a result of enzymatic analysis of the nmn splitting process, two types of enzyme responsible for this catabolism were ... | 1987 | 3034159 |
| the effect of cycloleucine on sfv a7(74) infection in mice. | cycloleucine (cl), a non-metabolizable amino acid analogue, was found to reduce thymus and spleen weights in semliki forest virus (sfv) strain a7(74) infected and control mice. the maximum effects were seen when three daily doses of cl were given to mice 24 h after an sfv a7(74) infection. in these mice thymus atrophy led to abolition of thymus dependent immune responses and changes in the pathological features of the viral infection--the most striking feature being prevention of demyelination. ... | 1987 | 3034321 |
| effects of procaine on pharmaco-mechanical coupling mechanisms activated by acetylcholine in smooth muscle cells of porcine coronary artery. | the action of procaine on pharmaco-mechanical coupling activated by application of acetylcholine (ach) was investigated using collagenase-treated dispersed intact and skinned smooth muscle cells and intact muscle tissues of the porcine coronary artery. ach reduced stored 45ca2+, and this action was prevented by procaine in intact dispersed cells. the maximum reduction in the level of stored 45ca induced by caffeine (25 mm) or inositol 1,4,5-trisphosphate (insp3; 3 microm) was also prevented by p ... | 1987 | 3034448 |
| open-chest cardiac massage after closed-chest compression in a canine model: when to intervene. | open-chest cardiac massage appears beneficial in improving hemodynamics during resuscitation efforts and in improving resuscitation success. the time between cardiac arrest and the initiation of open-chest cardiac massage is crucial. it would appear that if initiation of open chest cardiac massage is delayed for more than 20 min from the onset of cardiac arrest, little or no successful outcome can be expected. further techniques for assessing the adequacy of closed-chest compression cpr are need ... | 1987 | 3035670 |
| procollagenase activator produced by rabbit uterine cervical fibroblasts. | culture medium from rabbit uterine cervical fibroblasts contained a procollagenase and a neutral proproteinase which acts as a procollagenase activator. these two proenzymes have been purified by a combination of ion-exchange, affinity and gel chromatographies. the purified neutral proproteinase showed mr 60,000 with sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. this neutral proproteinase was activated by trypsin, 4-aminophenylmercuric acetate (apma) and plasmin, and the active spe ... | 1987 | 3036065 |
| a factor discriminating between the wild-type and a mutant polyomavirus enhancer. | enhancers increase the frequency of transcription initiation from linked promoter elements, most probably as a result of the binding of specific proteins to the enhancer. the polyomavirus early region is expressed in differentiated mouse cells but not in undifferentiated embryonal carcinoma (ec) cells. this host range is a function of the enhancer because polyomavirus mutants selected for growth in ec cells have mutations in the enhancer and the host range is reproduced in transfection assays us ... | 1987 | 3037380 |
| the pharmacokinetics of proguanil in human subjects following a single oral dose. | the pharmacokinetics of orally administered 200-mg dose of proguanil in volunteers, one african and one caucasian, is described. the drug was rapidly absorbed reaching a peak concentration in the blood within 3 h, and declining slowly thereafter to give a terminal phase elimination half life of 11.20 +/- 4.10 h and a systemic clearance of 1.270 +/- 0.020 l/h/kg. the small apparent volume of distribution shows that the drug is confined mainly to the blood and is not extensively bound to tissues; ... | 1987 | 3037875 |
| cloning in escherichia coli of the gene specifying the dna-entry nuclease of bacillus subtilis. | with the aim of cloning genes involved in transformation of bacillus subtilis, a set of transformation-deficient mutants was isolated by means of insertional mutagenesis with plasmid phv60 (vosman et al., 1986). analysis of these mutants showed that those mapping in the aroi region lacked the dna-entry nuclease activity. plasmid phv60 derivatives, containing flanking chromosomal dna fragments, were isolated from these mutants and were used to screen a library of b. subtilis chromosomal dna in ph ... | 1987 | 3038682 |
| cloning in escherichia coli of the gene specifying the dna-entry nuclease of bacillus subtilis. | with the aim of cloning genes involved in transformation of bacillus subtilis, a set of transformation-deficient mutants was isolated by means of insertional mutagenesis with plasmid phv60 (vosman et al., 1986). analysis of these mutants showed that those mapping in the aroi region lacked the dna-entry nuclease activity. plasmid phv60 derivatives, containing flanking chromosomal dna fragments, were isolated from these mutants and were used to screen a library of b. subtilis chromosomal dna in ph ... | 1987 | 3038682 |
| antagonist effects of the enantiomers of 3-ppp towards alpha 1-adrenoceptors coupled to inositol phospholipid breakdown in the rat cerebral cortex. | the effects of the two enantiomers of 3-ppp upon alpha 1-adrenergic and muscarinic receptors coupled to the inositol phospholipid (pi) breakdown response have been investigated. 3-ppp(-) and 3-ppp(+) were found to antagonize the noradrenaline (10 microm)-stimulated pi breakdown in rat cerebral cortical miniprisms with ic50 values of 18 and 61 microm, respectively. the dopamine receptor antagonists haloperidol and raclopride were also antagonists, with ic50 values of 0.4 and 25 microm, respective ... | 1987 | 3039482 |
| fulminant leukemic polyradiculoneuropathy in a case of b-cell prolymphocytic leukemia. a clinicopathologic report. | a 52 year old man with a 10-month history of b-cell prolymphocytic leukemia (pll) died of an apparent acute fulminant polyradiculoneuropathy, a condition generally attributed to paraneoplastic complication. the pathologic examination disclosed diffuse leukemic infiltrations of the peripheral nervous system. it is suggested that this particularly aggressive form of b-cell chronic prolymphocytic leukemia presented a constellation of features that promoted the invasion of the peripheral nervous sys ... | 1987 | 3040217 |
| analysis of transfer genes and gene products within the trab-trac region of the escherichia coli fertility factor, f. | a series of plasmids that carry overlapping segments of f dna encoding the genes in the trab-trac interval was constructed, and a restriction enzyme map of the region was derived. plasmids carrying deletions that had been introduced at an hpai site within this interval were also isolated. the ability of these plasmids to complement transfer of f lac plasmids carrying mutations in trab, trav, and traw, and trac was analyzed. the protein products of the plasmids were labeled in uv-irradiated cells ... | 1987 | 3040671 |
| [effect of bacterial toxins on microcirculation (experimental research)]. | histological and biomicroscopic methods were used to study the effects of toxic lemic and meningococcal microbial fractions on mesenteric blood microcirculation in rats. aggregation ability of red blood cells and thrombocytes was investigated. the sublethal dose of meningococcal toxins was shown to induce marked impairment in the blood microcirculation 30 minutes after intravenous administration. the lethal dose of lemic toxins manifests itself later (in 2-4 hours). meningococcal toxins are char ... | 1987 | 3105520 |
| [transport of basic amino acids into yersinia pestis cells]. | the transport of the basic amino-acids into the plague microbe cells has been shown to proceed by means of two transfer systems, the common transfer system and the system specific for lysine. besides that, arginine can be transported into the plague microbe cells by the histidine transport system. the vaccine strain ev niieg was used as a model to study the amino-acid transport systems. | 1987 | 3106795 |
| effects of neonatal estrogenization on rat bone development: a histomorphometric study. | the effects of a single dose of 500 micrograms of estradiol benzoate, administered on the first day of life, on rat bone development have been histomorphometrically studied at 15 days of age. estrogenized animals presented decreased total tibial length (16.55 +/- 0.50 vs. 17.84 +/- 0.73 mm, p less than 0.05) and increased thickness of the cartilage growth plate (528.92 +/- 13.30 vs. 382.77 +/- 37.85 micron, p less than 0.01). this increase was mostly due to the presence of a wider (p less than 0 ... | 1987 | 3107771 |
| reflex regulation of atrioventricular conduction. | we evaluated the time course of baroreflex modulation of atrioventricular (av) nodal conduction in anesthetized dogs (n = 28). beat-by-beat changes in heart rate (hr) and av interval (avi) evoked by transient alterations in arterial pressure (ap) were recorded in the intact state, after vagotomy, and following stellectomy. under each experimental condition, alterations in ap induced parallel changes in hr and avi with maximum hr and avi responses occurring simultaneously. in three animals, ap al ... | 1987 | 3109256 |