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[investigation of antibacterial activity of sertralin].sertralin is a psychotropic drug which acts by inhibiting the selective serotonin re-uptake in the synaptic area. previous studies have shown that some antidepressant agents have antibacterial activity. the aim of this study was to investigate the in vitro antibacterial activity of sertralin. a total of 224 bacterial strains isolated from clinical specimens together with standard control strains were included to the study. the antibacterial activity of sertralin was determined by microdilution m ...200920084919
synthesis and antibacterial assessment of n-[4-(4-substituted phenyl)-1,3-thiazol-2-yl]-1,3,5-triazin-2-amine.in a wide search program towards new and efficient antibacterial agents, we assessed the extent to which physicochemical properties can be exploited to promote antibacterial activity associated with a series of substituted s-triazine. the synthesized compounds (1a-12b) were subsequently screened for their in vitro antibacterial activity against three gram positive (bacillus subtilis, bacillus cereus, staphylococcus aureus) and three gram-negative microorganism (salmonella typhi, escherichia coli ...200920177467
clinical characteristics and predictors of mortality in patients with enterobacter aerogenes bacteremia.enterobacter aerogenes is increasingly encountered in nosocomial infections. this study aimed to clarify the clinical characteristics and to identify the predictors of mortality in patients with e. aerogenes bacteremia.200919949757
trends in antimicrobial susceptibility of gram-negative bacteria isolated from blood in jakarta from 2002 to 2008.this study examined the susceptibility of gram-negative bacteria in the bloodstream to antimicrobials with the aim of providing information relevant to the guidance of therapy.200920061679
infection of brindley sacral anterior root stimulator by pseudomonas aeruginosa requiring removal of the implant: long-term deleterious effects on bowel and urinary bladder function in a spinal cord injury patient with tetraplegia: a case report.we report infection of brindley sacral anterior root stimulator in a spinal cord injury patient, who ultimately required removal of the implant. the consequences of failed implantation were severe constipation, and loss of reflex penile erection and bladder emptying.200920062610
significance of infection markers and microbiological findings during tissue processing of cryopreserved arterial homografts for the early postoperative course.to evaluate homograft implantation for the urgent treatment of vascular infections on the basis of the course of infection using microbiological findings in perioperatively obtained specimens and during homograft processing.200919998257
bacteriological evaluation of pre-cut fruits sold in kano metropolis, kano state, nigeria.one hundred and fifty (150) pre-cut fruit samples comprising of pineapples (50), paw-paw (50) and watermelon (50) at the point of stand retail outlets were tested by standard bacteriological methods to determine bacterial contamination of the fruits. out of these 150 examined 136 (90.67%) were contaminated with bacteria. the bacterial distribution were; escherichia coli 69 (46.00%), staphylococcus aureus 29 (19.33%), salmonella species 13 (8.67%), proteus species 18 (12.00%), enterobacter aeroge ...200920000065
antibacterial activity of leaves of cadaba trifoliata (roxb.) wt. & arn.antibacterial activity of aqueous and ethanol leaf extracts of cadaba trifoliata was evaluated by cup plate method against bacterial strains such as staphylococcus aureus, bacillus subtilis, acinetobacter, enterobacter aerogenes, erwinia and escherichia coli. the ethanol extract of the leaves demonstrated a high degree of activity against all the tested bacterial strains except erwinia and acinetobacter, whereas the aqueous extract of the leaves showed moderate activity against e. coli, b. subti ...200920336203
screening of antibacterial potentials of some medicinal plants from melghat forest in india.cyperus rotundus, caesalpinia bonducella, tinospora cordifolia, gardenia gummifera, ailanthus excelsa, acacia arabica, embelia ribes and ventilago maderspatana from melghat forest were screened for their antibacterial potential against escherichia coli, staphylococcus aureus, klebsiella pneumoniae, proteus vulgaris, salmonella typhi, shigella flexneri, salmonella paratyphi, salmonella typhimurium, pseudomonas aeruginosa, enterobacter aerogenes by disc diffusion method. out of these medicinal pla ...200920448847
determination of antibacterial and antioxidant potential of some medicinal plants from saurashtra region, india.many plants used in saurashtra folk medicine have been reported to exhibit high antibacterial and antioxidant activities. in the present study, some parts of five plants, guazuma ulmifolia l., manilkara zapota l., melia azedarach l., syzygium cumini l. and wrightia tomentosa r.& s., were evaluated for their antibacterial activity, total phenol content, flavonoid content, 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity and phytochemical analysis, using successive extraction by cold ...200920502546
strategies for acquiring the phospholipid metabolite inositol in pathogenic bacteria, fungi and protozoa: making it and taking it.myo-inositol (inositol) is an essential nutrient that is used for building phosphatidylinositol and its derivatives in eukaryotes and even in some eubacteria such as the mycobacteria. as a consequence, fungal, protozoan and mycobacterial pathogens must be able to acquire inositol in order to proliferate and cause infection in their hosts. there are two primary mechanisms for acquiring inositol. one is to synthesize inositol from glucose 6-phosphate using two sequentially acting enzymes: inositol ...200919383710
detection of plasmid-mediated quinolone resistance genes in clinical isolates of enterobacter spp. in spain.we have studied by pcr and dna sequencing the presence of the qnra, qnrb, qnrs, aac(6')-ib-cr, qepa, inti1, and iscr1 genes in 200 clinical isolates of enterobacter cloacae (n = 153) and e. aerogenes (n = 47) consecutively collected between january 2004 and october 2005 in two hospitals located in santander (northern spain) and seville (southern spain). mutations in the quinolone resistance-determining region of gyra and parc also were investigated in organisms containing plasmid-mediated quinol ...200919386836
biological activity of alkaloids from solanum dulcamara l.alkaloids are well known for their antimicrobial activity. though all natural alkaloids come from plants, not all plants produce alkaloids. plants of the solanaceae family are known for their high alkaloid content. alkaloids are found in all plant parts like roots, stems, leaves, flowers, fruits and seeds. in the present study, those plant parts of solanum dulcamara were selected which have been reported to produce a high content of a specific alkaloid: solanine (from unripe fruits), solasodine ...200919418354
how beta-lactam antibiotics enter bacteria: a dialogue with the porins.multi-drug resistant (mdr) infections have become a major concern in hospitals worldwide. this study investigates membrane translocation, which is the first step required for drug action on internal bacterial targets. beta-lactams, a major antibiotic class, use porins to pass through the outer membrane barrier of gram-negative bacteria. clinical reports have linked the mdr phenotype to altered membrane permeability including porin modification and efflux pump expression.200919434239
incidence of multiple antibiotic resistant gram-negative bacteria isolated from surface and underground water sources in south western region of nigeria.in most rural and urban settlements, particularly in nigeria, wells, spring, streams or rivers and lakes serves as major sources of water supply for drinking and other domestic purposes. unfortunately, many of the available water sources are not potable without some form of treatment which is seldom available in most settings. the use of untreated surface water sources for drinking and for domestic purposes remains a major threat to public health as these could serve as reservoirs the for transf ...200919474486
copper uptake by pseudomonas aeruginosa isolated from infected burn patients.pseudomonas aeruginosa was isolated from infected burn patients and characterized by standard biochemical tests. the in vitro copper uptake was compared between this isolated pathogenic strain and two non-pathogenic control strains of gram positive bacteria bacillus thuringiensis strain israelis as well as gram negative bacteria enterobacter aerogenes. maximum copper uptake of 470 ppm/g biomass was obtained by p. aeruginosa strain, while the control strains b. thuringiensis and enterobacter aero ...200919484296
role of metal resistant plant growth promoting bacteria in ameliorating fly ash to the growth of brassica juncea.in this study, we have shown that the plant growth promoting bacterial strain nbri k24 and strain nbri k3 from fly ash (fa) contaminated soil reduce the toxicity of ni and cr in brassica juncea (indian mustard) and promote plant growth under pot culture experiments. isolated strains nbri k24 and nbri k3 were characterized based on the 16s rdna sequencing and identified as enterobacter aerogenes and rahnella aquatilis respectively. both the strains were siderophore producing and found capable of ...200919487076
influence of microbial growth on the redox potential of fermented cucumbers.commonly, ph measurements are used during the production of fermented cucumbers to indirectly monitor growth of lactic acid bacteria (lab) and acid production. redox potential (e(h)) measurements, which are determined by the potential of an electron to reduce an acceptor, could serve as an alternative tool to monitor the progress of fermentation allowing the detection of the metabolic activity and/or growth of lab and other microorganisms. pasteurized and inoculated jars of cucumbers were observ ...200919490331
sepsis resulting from enterobacter aerogenes resistant to carbapenems after liver transplantation.sepsis due to enterobacter aerogenes (e. aerogenes) is rare after liver transplantation but is also a serious infection that may cause liver abscess. the purpose of this case report is to relate an unusual presentation of liver transplantation to show how successive treatment can be an appropriate option in septic patients after liver transplantation.200919502176
expression of nad+-dependent formate dehydrogenase in enterobacter aerogenes and its involvement in anaerobic metabolism and h2 production.an expression system for nad(+)-dependent formate dehydrogenase gene (fdh1), from candida boidinii, was constructed and cloned into enterobacter aerogenes iam1183. with the fdh1 expression, the total h(2) yield was attributed to a decrease in activity of the lactate pathway and an increase of the formate pathway flux due to the nadh regeneration. analysis of the redox state balance and ethanol-to-acetate ratio in the fdhl-expressed strain showed that increased reducing power arose from the recon ...200919533026
[mechanisms of bioelectricity generation in enterobacter aerogenes-based microbial fuel cells].microbial fuel cells (mfcs) using hydrogen-producing bacteria (hpb) could utilize a large number of substrates to generate power. however, the coulombic efficiency is limited by the fact that only suspended cells are used as biocatalyst in anodic medium. mfcs using fe (iii)-reducing bacteria have high energy recovery efficiency, but can only utilize some simple organic matters. in this study, enterobacter aerogenes xm02, a hydrogen-producing strain with fe(iii)-reducing activity, was selected as ...200919545032
investigation and control of an outbreak of enterobacter aerogenes bloodstream infection in a neonatal intensive care unit in fiji.ten neonates developed blood stream infection with extended-spectrum beta-lactamase-producing enterobacter aerogenes in a neonatal intensive care unit in fiji. the source of the outbreak was traced to a bag of contaminated normal saline in the ward, which was used for multiple patients. all isolates recovered from patients were indistinguishable from the bacteria recovered from the normal saline by pulsed-field gel electrophoresis. the outbreak was controlled using simple infection control pract ...200919552517
synthesis, nematicidal and antimicrobial properties of bis-[4-methoxy-3-[3-(4-fluorophenyl)-6-(4-methylphenyl)-2(aryl)-tetrahydro-2h-pyrazolo[3,4-d]thiazol-5-yl]phenyl]methanes.a new series of bis-[4-methoxy-3-[3-(4-fluorophenyl)-6-(4-methylphenyl)-2-(aryl)-3,3a,5,6-tetrahydro-2h-pyrazolo[3,4-d][1,3]thiazol-5-yl]phenyl]methanes 6a-r was synthesized by the reaction of arylidine derivative of methylene-bis-thiazolidinones 5a-c with aryl/alkyl hydrazines. chemical structures of all the new compounds were established by ir, (1)h-nmr, (13)c-nmr, ms and elemental data. the compounds 6a-r were evaluated for their nematicidal activity against ditylenchus myceliophagus and caen ...200919571412
combining capillary electrophoresis with laser-induced fluorescence detection for the analysis of escherichia coli lysates.with the goal of improving water quality control, in this study we combined ce-lif for the analysis of escherichia coli lysates under discontinuous conditions. prior to injecting a large-volume protein sample, a plug of 0.2% w/v sds was injected into the capillary filled with 2.0 m tris-borate (tb) solution (ph 10.3). during the courses of the process stacking, proteins migrated against the electroosmotic flow (eof) and entered a 1.7% w/v poly(ethylene oxide) solution that has been prepared in 2 ...200919621366
failure of the microscan walkaway system to detect heteroresistance to carbapenems in a patient with enterobacter aerogenes bacteremia.we report the failure of the automated microscan walkaway system to detect carbapenem heteroresistance in enterobacter aerogenes. carbapenem resistance has become an increasing concern in recent years, and robust surveillance is required to prevent dissemination of resistant strains. reliance on automated systems may delay the detection of emerging resistance.200919641071
structural flexibility enhances the reactivity of the bioremediator glycerophosphodiesterase by fine-tuning its mechanism of hydrolysis.the glycerophosphodiesterase from enterobacter aerogenes (gpdq) belongs to the family of binuclear metallohydrolases and has attracted recent attention due to its potential in bioremediation. formation of a catalytically competent binuclear center is promoted by the substrate (hadler et al. j. am. chem. soc. 2008, 130, 14129). using the paramagnetic properties of mn(ii), we estimated the k(d) values for the metal ions in the alpha and beta sites to be 29 and 344 microm, respectively, in the abse ...200919653693
huge symbiotic organs in giant scale insects of the genus drosicha (coccoidea: monophlebidae) harbor flavobacterial and enterobacterial endosymbionts.giant scale insects (drosicha: coccoldea: monophlebidae) were investigated for their symbiotic organs and bacterial endosymbionts. two types of bacterial 16s rrna gene sequences, flavobacterial and enterobacterial, were consistently detected in d. corpulenta and d. pinicola. the former sequences formed a compact clade in the bacteroidetes, allied to the symbionts of cushion and armored scales. the latter sequences formed a robust clade in the gamma-proteobacteria, allied to enteric bacteria like ...200919663639
antibacterial activity of ethanol extracts of seaweeds against fish bacterial pathogens.crude extracts were prepared using the solvent ethanol from the seaweeds viz., gracilaria edulis, calorpha peltada and hydroclothres sp. and screening for their antibacterial activity against six bacterial pathogens were also carried out. the test bacterial strains were escherichia coli, enterobacter aerogenes, staphylococcus aureus, pseudomonas aeruginosa, streptococcus faecalis and bacillus cereus. ethanol extract of gracilaria edulis inhibited growth of all the test organisms except bacillus ...200919673167
synthesis of n-tetra-o-acetyl-beta-d-glucopyranosyl-n'-(4',6'-diarylpyrimidin-2'-yl)thioureas.some 2-amino-4,6-diarylpyrimidines 2 have been prepared from substituted benzylideneacetophenones and guanidine hydrochloride in the presence of alkali by conventional heating in alcoholic medium and microwave heating in solvent-free conditions. n-(2,3,4,6-tetra-o-acetyl-beta-d-glucopyranosyl)-n'-(4',6'-diarylpyrimidin-2'-yl)thioureas 4 have been synthesized by reaction of per-o-acetylated glucopyranosyl isothiocyanate 1 and substituted 2-amino-4,6-diarylpyrimidines 2. two different methods have ...200919804880
chemical composition and antimicrobial activity of the extracts of kefe cumin (laser trilobum l.) fruits from different regions.structural properties (using gas chromatographic analyses: gas chromatography and gas chromatography-mass spectrometry) of some compounds (pure chemicals, essential oils, extracts, etc.) and systematic investigation of antimicrobial activities (with agar dilution and agar diffusion disc methods) were determined in the fruits of kefe cumin (laser trilobum l.) obtained from different regions (germany, india, and turkey). the main components were established as limonene (41.03-72.24%) and perillald ...200919817640
tonb-dependent transporters and their occurrence in cyanobacteria.different iron transport systems evolved in gram-negative bacteria during evolution. most of the transport systems depend on outer membrane localized tonb-dependent transporters (tbdts), a periplasma-facing tonb protein and a plasma membrane localized machinery (exbbd). so far, iron chelators (siderophores), oligosaccharides and polypeptides have been identified as substrates of tbdts. for iron transport, three uptake systems are defined: the lactoferrin/transferrin binding proteins, the porphyr ...200919821963
the in-vitro antimicrobial activity of some medicinal plants against beta-lactam-resistant bacteria.in effort to identify novel bacterial agents, this study was initiated to evaluate the antimicrobial properties of 17 crude extracts from 12 medicinal plants against beta-lactam-resistant bacteria.200919858568
assessment of microbiological quality of sachet-packaged drinking water in western nigeria and its public health significance.to assess the microbiological quality of sachet-packaged drinking water in western nigeria and its impact on public health.200919880150
magnetic circular dichroism study of a dicobalt(ii) complex with mixed 5- and 6-coordination: a spectroscopic model for dicobalt(ii) hydrolases.the magnetic circular dichroism (mcd) study of [co(2)(mu-oh)(mu-ph(4)dba)(tmeda)(2)(otf)], in which ph(4)dba is the dinucleating bis(carboxylate) ligand dibenzofuran-4,6-bis(diphenylacetate) and tmeda is n,n,n',n'-tetramethylethylenediamine, is presented. this complex serves as an excellent spectroscopic model for a number of dicobalt(ii) enzymes and proteins that have both the mu-hydroxo, mu-carboxylato bridging and asymmetric 6- and 5-coordination. the low-temperature mcd spectrum of the model ...200919691327
genetic structure and distribution of the colibactin genomic island among members of the family enterobacteriaceae.a genomic island encoding the biosynthesis and secretion pathway of putative hybrid nonribosomal peptide-polyketide colibactin has been recently described in escherichia coli. colibactin acts as a cyclomodulin and blocks the eukaryotic cell cycle. the origin and prevalence of the colibactin island among enterobacteria are unknown. we therefore screened 1,565 isolates of different genera and species related to the enterobacteriaceae by pcr for the presence of this dna element. the island was dete ...200919720753
use of boronic acid disk tests to detect extended- spectrum beta-lactamases in clinical isolates of kpc carbapenemase-possessing enterobacteriaceae.we evaluated boronic acid (ba)-based methods for their ability to detect extended-spectrum beta-lactamases (esbls) among clinical isolates of kpc-producing members of the enterobacteriaceae family. a total of 155 isolates of klebsiella pneumoniae (n = 141), escherichia coli (n = 6), enterobacter aerogenes (n = 6), and klebsiella oxytoca (n = 2) genotypically confirmed to be kpc producers were analyzed. as many as 118 isolates harbored esbls (103 harbored shv-type esbls, 13 harbored ctx-m-type es ...200919726597
heparin/heparan sulfate 6-o-sulfatase from flavobacterium heparinum: integrated structural and biochemical investigation of enzyme active site and substrate specificity.heparin and heparan sulfate glycosaminoglycans (hsgags) comprise a chemically heterogeneous class of sulfated polysaccharides. the development of structure-activity relationships for this class of polysaccharides requires the identification and characterization of degrading enzymes with defined substrate specificity and enzymatic activity. toward this end, we report here the molecular cloning and extensive structure-function analysis of a 6-o-sulfatase from the gram-negative bacterium flavobacte ...200919726671
heparin/heparan sulfate n-sulfamidase from flavobacterium heparinum: structural and biochemical investigation of catalytic nitrogen-sulfur bond cleavage.sulfated polysaccharides such as heparin and heparan sulfate glycosaminoglycans (hsgags) are chemically and structurally heterogeneous biopolymers that that function as key regulators of numerous biological functions. the elucidation of hsgag fine structure is fundamental to understanding their functional diversity, and this is facilitated by the use of select degrading enzymes of defined substrate specificity. our previous studies have reported the cloning, characterization, recombinant express ...200919726673
chemical composition, antifungal and antibacterial activity of the essential oil of chamaecyparis nootkatensis from spain.the chemical composition of the oil of chamaecyparis nootkatensis (d. don) spach. has been analysed by gas chromatography (gc) and gas chromatography coupled to mass spectrometry (gc-ms). a total of 34 compounds were identified representing more than 90% of the total oil. the oil was richer in monoterpenes than in sesquiterpenes, the major constituents being limonene (53.2%), 6-3-carene (21.0%) and a-pinene (12.2%). the antibacterial and antifungal activities of the oil were also tested against ...200919731613
extended-spectrum beta-lactamases among enterobacteriaceae isolated in a public hospital in brazil.extended-spectrum beta-lactamases (esbl) in enterobacteria are recognized worldwide as a great hospital problem. in this study, 127 esbl-producing enterobacteriaceae isolated in one year from inpatients and outpatients at a public teaching hospital at são paulo, brazil, were submitted to analysis by pcr with specific primers for bla shv, bla tem and bla ctx-m genes. from the 127 isolates, 96 (75.6%) klebsiella pneumoniae, 12 (9.3%) escherichia coli, 8 (6.2%) morganella morganii, 3 (2.3%) proteus ...200919739000
biological properties of the chilean native moss sphagnum magellanicum.an ethanol extract prepared from the gametophyte chilean native moss sphagnum magellanicum was dried out, weighed and dissolved in distilled water. this extract was then assayed for its antibacterial activity against the g(-) bacteria azotobacter vinelandii, erwinia carotovora subsp. carotovora, enterobacter aerogenes, escherichia coli, pseudomonas aeruginosa, salmonella typhi, vibrio cholerae, and the g(+) bacteria staphylococcus aureus subsp. aureus, and streptococcus type beta. the growth of ...200919746269
a genome-scale metabolic model of arabidopsis and some of its properties.we describe the construction and analysis of a genome-scale metabolic model of arabidopsis (arabidopsis thaliana) primarily derived from the annotations in the aracyc database. we used techniques based on linear programming to demonstrate the following: (1) that the model is capable of producing biomass components (amino acids, nucleotides, lipid, starch, and cellulose) in the proportions observed experimentally in a heterotrophic suspension culture; (2) that approximately only 15% of the availa ...200919755544
isolation of salmonella and shigella from fish harvested from the winam gulf of lake victoria, kenya.human infections caused by pathogens transmitted from fish are quite common. the aim of this study was to isolate enteric pathogenic bacteria from fish that might be transmitted to humans after the handling or consumption of such fish.200919755738
[isolation and identification of degradation bacteria enterobacter aerogenes for pyrethriods pesticide residues and its degradation characteristics].by incubation experiment, the bacterial strain labeled as m6r9 was isolated from the tame sludge in water course of pesticide factory of hangzhou, and was identified as enterobacter aerogenes, which had highly efficient degradation for bifenthrin, fenpropathrin and cypermethrin. by investigating the physiological characteristics of the strain, the results show that the bacterium is a gram-negative aerobe bacilli, size is (0.8-1.9) microm x (0.5-1.0) microm, and is capable of utilizing bifenthrin ...200919799315
role of intracellular polysaccharide in persistence of streptococcus mutans.intracellular polysaccharide (ips) is accumulated by streptococcus mutans when the bacteria are grown in excess sugar and can contribute toward the cariogenicity of s. mutans. here we show that inactivation of the glga gene (smu1536), encoding a putative glycogen synthase, prevented accumulation of ips. ips is important for the persistence of s. mutans grown in batch culture with excess glucose and then starved of glucose. the ips was largely used up within 1 day of glucose starvation, and yet s ...200919801415
bacterial contamination in drinking water: a case study in rural areas of northern rajasthan, india.the objective of this study was to determine the bacterial contaminations in drinking water samples collected form some rural habitations of northern rajasthan, india. a total of ten bacterial species: escherichia coli, pseudomonas aeruginosa, enterobacter aerogenes, klebsiella sp, proteus vulgaris, alcaligenes faecalis, bacillus cereus, staphylococcus aureus, streptococcus lactis and micrococcus luteum were identified form drinking water samples. the bacteria belonging to the family enterobacte ...200919023670
absorption, distribution, metabolism, and excretion of 2,2-bis(bromomethyl)-1,3-propanediol in male fischer-344 rats.2,2-bis(bromomethyl)-1,3-propanediol (bmp) is a brominated flame retardant, previously shown to be a multisite carcinogen in experimental animals. studies were performed to characterize the dispositional and metabolic fate of bmp after oral or intravenous administration to male fischer-344 rats. after a single oral administration of [(14)c]bmp (10 or 100 mg/kg) >80% of the low dose and 48% of the high dose were excreted by 12 h in the urine predominantly as a glucuronide metabolite. after repeat ...200919029203
absorption, distribution, metabolism, and excretion of 2,2-bis(bromomethyl)-1,3-propanediol in male fischer-344 rats.2,2-bis(bromomethyl)-1,3-propanediol (bmp) is a brominated flame retardant, previously shown to be a multisite carcinogen in experimental animals. studies were performed to characterize the dispositional and metabolic fate of bmp after oral or intravenous administration to male fischer-344 rats. after a single oral administration of [(14)c]bmp (10 or 100 mg/kg) >80% of the low dose and 48% of the high dose were excreted by 12 h in the urine predominantly as a glucuronide metabolite. after repeat ...200919029203
microbiological and toxicological effects of perla black bean (phaseolus vulgaris l.) extracts: in vitro and in vivo studies.we investigated the microbiological and toxicological effects of three perla black bean extracts on the growth and culture of selected pathogenic microorganisms, the toxicity over vero cell lines and an in vivo rat model. three different solvents were used to obtain perla black bean extracts. all three perla black bean extracts were tested for antibacterial and antiparasitic activity and further analysed for intrinsic cytotoxicity (ic(50)). methanol perla black bean extract was used for acute to ...200919053992
purine utilization by klebsiella oxytoca m5al: genes for ring-oxidizing and -opening enzymes.the enterobacterium klebsiella oxytoca uses a variety of inorganic and organic nitrogen sources, including purines, nitrogen-rich compounds that are widespread in the biosphere. we have identified a 23-gene cluster that encodes the enzymes for utilizing purines as the sole nitrogen source. growth and complementation tests with insertion mutants, combined with sequence comparisons, reveal functions for the products of these genes. here, we report our characterization of 12 genes, one encoding gua ...200919060149
purine utilization by klebsiella oxytoca m5al: genes for ring-oxidizing and -opening enzymes.the enterobacterium klebsiella oxytoca uses a variety of inorganic and organic nitrogen sources, including purines, nitrogen-rich compounds that are widespread in the biosphere. we have identified a 23-gene cluster that encodes the enzymes for utilizing purines as the sole nitrogen source. growth and complementation tests with insertion mutants, combined with sequence comparisons, reveal functions for the products of these genes. here, we report our characterization of 12 genes, one encoding gua ...200919060149
ampc beta-lactamases.summary: ampc beta-lactamases are clinically important cephalosporinases encoded on the chromosomes of many of the enterobacteriaceae and a few other organisms, where they mediate resistance to cephalothin, cefazolin, cefoxitin, most penicillins, and beta-lactamase inhibitor-beta-lactam combinations. in many bacteria, ampc enzymes are inducible and can be expressed at high levels by mutation. overexpression confers resistance to broad-spectrum cephalosporins including cefotaxime, ceftazidime, an ...200919136439
enzymatic transesterification of jatropha oil.transesterification of jatropha oil was carried out in t-butanol solvent using immobilized lipase from enterobacter aerogenes. the presence of t-butanol significantly reduced the negative effects caused by both methanol and glycerol. the effects of various reaction parameters on transesterification of jatropha oil were studied.200919144158
occurrence of efflux mechanism and cephalosporinase variant in a population of enterobacter aerogenes and klebsiella pneumoniae isolates producing extended-spectrum beta-lactamases.we investigated the occurrence of multidrug resistance in 44 enterobacter aerogenes and klebsiella pneumoniae clinical isolates. efflux was involved in resistance in e. aerogenes isolates more frequently than in k. pneumoniae isolates (100 versus 38% of isolates) and was associated with the expression of phenylalanine arginine beta-naphthylamide-susceptible active efflux. acra-tolc overproduction in e. aerogenes isolates was noted. an analysis of four e. aerogenes isolates for which cefepime mic ...200919164140
blood culture confirmed bacterial sepsis in neonates in a north indian tertiary care center: changes over the last decade.the spectrum of organisms causing sepsis is different in developing countries. data on the recent trends of organisms causing sepsis are limited. this study was conducted in a tertiary care neonatal unit in northern india. all inborn babies with blood-culture-positive sepsis from 1995 to 2006 were divided into two epochs, viz. 1995 to 1998 (epoch i) and 2001 to 2006 (epoch ii). organisms were grouped into early (<72 h) and late onset (> or =72 h) sepsis groups. the overall incidence of sepsis, t ...200919168958
antimicrobial activity of some thiourea derivatives and their nickel and copper complexes.five thiourea derivative ligands and their ni(2+) and cu(2+) complexes have been synthesized. the compounds were screened for their in vitro anti-bacterial activity using gram-positive bacteria (two different standard strains of staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, streptococcus pyogenes, bacillus cereus) and gram-negative bacteria (esherichia coli, pseudomonas aeruginosa, enterobacter cloacae, proteus vulgaris, enterobacter aerogenes) and in vitro anti-yeast ...200919169199
isothiocyanate profile and selective antibacterial activity of root, stem, and leaf extracts derived from raphanus sativus l.acetone and hexane extracts derived from the root, stem, and leaf of raphanus sativus were investigated for their antibacterial activity against foodborne and resistant pathogens, such as bacillus subtilis, staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, salmonella typhimurium, enterobacter aerogenes, enterobacter cloacae, and escherichia coli. total and individual isothiocyanate (itc) components and their relationship with the antibacterial activity of r. sativus were ...200919182965
identification of [14c]fluasterone metabolites in urine and feces collected from dogs after subcutaneous and oral administration of [14c]fluasterone.the objective of this research was the identification of the metabolic profile of fluasterone, a synthetic derivative of dehydroepiandrosterone, in dogs treated orally or subcutaneously with [4-(14)c]fluasterone. separation and characterization techniques used to identify the principal metabolites of fluasterone in urine and feces included high-performance liquid chromatography (hplc), liquid scintillation spectrometry, hplc/tandem mass spectrometry, and nmr. in urine, the majority of the radioa ...200919196848
accumulation of plasmid-mediated fluoroquinolone resistance genes, qepa and qnrs1, in enterobacter aerogenes co-producing rmtb and class a beta-lactamase lap-1.a new plasmid-mediated fluoroquinolone efflux pump gene, qepa, is known to be associated with the rmtb gene, which confers high-level resistance to aminoglycosides. we investigated the qepa gene in 573 ampc-producing enterobacteriaceae including one citrobacter freundii known to harbor rmtb. of them, two clonally unrelated e. aerogenes harbored qepa. both isolates co-harbored rmtb, qnrs1, qepa, and bla(lap-1) on an incfi type plasmid. the qepa was flanked by two copies of is26 containing iscr3c, ...200919201742
molecular mechanisms disrupting porin expression in ertapenem-resistant klebsiella and enterobacter spp. clinical isolates from the uk.the aim of this study was to investigate relatedness and molecular mechanism(s) of ertapenem resistance in clinical isolates of klebsiella spp. (n = 28) and enterobacter spp. (n = 27) referred from multiple hospitals to the uk national reference laboratory.200919233898
role of tolc in klebsiella oxytoca resistance to antibiotics.the gram-negative human pathogen klebsiella oxytoca is often resistant to several antibiotics such as fluoroquinolones, erythromycin, tetracycline, chloramphenicol and others. the aim of this study was to look at the mechanisms leading to this resistance and particularly the role of tolc and efflux mechanisms in determining resistance.200919240073
optimization of the enzymatic hydrolysis and analysis of plasma conjugated gamma-cehc and sulfated long-chain carboxychromanols, metabolites of vitamin e.natural forms of vitamin e are metabolized by omega-hydroxylation and beta-oxidation of the hydrophobic side chain to generate urinary-excreted 2-(beta-carboxyethyl)-6-hydroxychroman (cehc) and cehc conjugates (sulfate, glucuronide, or glucoside). we recently showed that sulfated long-chain carboxychromanols, the conjugated intermediate beta-oxidation products, are formed from tocopherols and tocotrienols in human cells and in rats. cehc conjugates have been quantified after being converted to i ...200919250920
geraniol restores antibiotic activities against multidrug-resistant isolates from gram-negative species.the essential oil of helichrysum italicum significantly reduces the multidrug resistance of enterobacter aerogenes, escherichia coli, pseudomonas aeruginosa, and acinetobacter baumannii. combinations of the two most active fractions of the essential oil with each other or with phenylalanine arginine beta-naphthylamide yield synergistic activity. geraniol, a component of one fraction, significantly increased the efficacy of beta-lactams, quinolones, and chloramphenicol.200919258278
synthesis, biological evaluation, mechanism of action and quantitative structure-activity relationship studies of chalcones as antibacterial agents.forty-eight chalcone analogs were synthesized and their in vitro antibacterial activity against staphylococcus aureus ncim 5021, bacillus subtilis ncim 2718, phaseolus vulgaris ncim 2813, escherichia coli ncim 2931, salmonella typhi 2501 and enterobacter aerogenes ncim 5139 were evaluated by microdilution broth assay. quantitative structure-activity relationships were developed for all the cases (r(2) = 0.68-0.79; r(2)(adj) = 0.58-0.78; q(2) = 0.51-0.68; f = 13.02-61.51). size, polarizability, e ...200919291103
novel ctx-m {beta}-lactamase genotype distribution and spread into multiple species of enterobacteriaceae in changsha, southern china.the aim of this study was to undertake a survey of the occurrence of ctx-m and shv extended-spectrum beta-lactamase (esbl) genotypes in enterobacteriaceae from hunan province, china.200919297379
effects of an alternative cefepime dosing strategy in pulmonary and bloodstream infections caused by enterobacter spp, citrobacter freundii, and pseudomonas aeruginosa: a single-center, open-label, prospective, observational study.various dosing strategies for cefepime have been developed in an effort to maximize pharmacodynamic exposure of this agent against gram-negative infections. an assessment of cefepime dosing strategies is warranted given recent reports of poorer treatment outcomes associated with cefepime compared with other antibiotics, particularly in patients infected with gram-negative organisms with elevated mics.200919302902
regulation of glycerol metabolism in enterobacter aerogenes nbrc12010 under electrochemical conditions.enterobacter aerogenes nbrc12010 was able to ferment glycerol to ethanol and hydrogen gas. fermentation of glycerol ceased in the stationary phase of growth, and it was activated by electrochemical reactions using thionine as an electron transfer mediator from bacterial cells to an electrode. using resting cells of e. aerogenes nbrc12010 in only citrate buffer solution, the cells did not consume glycerol at all, but they could metabolize glucose. these results suggest that the regulation of glyc ...200919352646
the bioremediator glycerophosphodiesterase employs a non-processive mechanism for hydrolysis.glycerophosphodiesterase (gpdq) from enterobacter aerogenes is a binuclear metallohydrolase that catalyzes the breakdown of a broad range of phosphate ester substrates, and it is of interest for its potential application in the destruction of organophosphate nerve agents and pesticides. the reaction mechanism of gpdq has been proposed to involve a nucleophilic attack by a terminally bound hydroxide molecule. the hydroxide species bridging the two metal ions is suggested to activate the nucleophi ...201019923005
relationship between structure and antibacterial activity of lipophilic n-acyldiamines.we report in this work the preparation and antibacterial evaluation of a series of n-monoacylated diamines against six gram-positive and 11 gram-negative bacteria. the results obtained showed the existence of relationship between lipophilicity and antibacterial activity of the tested compounds. the best results were obtained against gram-positive bacteria for compounds having a 10-12 carbons alkyl chain. compound 4e was the most active against microccus lentus (mic=2 microg/ml), staphylococcus a ...201019942397
alteration of hydrogen metabolism of ldh-deleted enterobacter aerogenes by overexpression of nad+-dependent formate dehydrogenase.the nad+-dependent formate dehydrogenase fdh1 gene (fdh1), cloned from candida boidinii, was expressed in the ldh-deleted mutant of enterobacter aerogenes iam1183 strain. the plasmid of pcom10 driven by the palkb promoter was used to construct the fdh1 expression system and thus introduce a new dihydronicotinamide adenine dinucleotide (nadh) regeneration pathway from formate in the ldh-deleted mutant. the knockout of nadh-consuming lactate pathway affected the whole cellular metabolism, and the ...201019830418
growth and gaseous emissions of pure and mixed small intestinal bacterial cultures: effects of bile and vancomycin.simultaneous cultivations in anaerobiosis, aerobiosis and with microaerobic gas mixture were used to clarify the bile (oxgall) effects on the pure and mixed cultures of enterobacterial strains in simulations in portable microbe enrichment unit (pmeu) linked with chempro100i((r)) gas detector. the effects of vancomycin were evaluated in aerobic cultures. growth and metabolic activity of cultures were also followed by measuring sugar consumption, ph alterations, and colony counts on bd chromagar o ...201019682874
isolation of bacteriocin-like substances producing bacteria from finished cattle-manure compost and activity evaluation against some food-borne pathogenic and spoilage bacteria.a finished compost sample was examined for bacteriocin-like substance production against five pathogenic bacteria: salmonella typhimurium ef 85-9, escherichia coli o157:h7 atcc 43888, enterococcus faecalis jcm 8726, staphylococcus aureus jcm 2151, and yersinia enterocolitica jcm 7577. at the preliminary detection of bacterial strains exhibiting antimicrobial activity from the compost sample, thirteen strains could be isolated. screening of the inhibitory activity was done using agar-well diffusi ...201020513963
mutagenesis of klebsiella aerogenes ureg to probe nickel binding and interactions with other urease-related proteins.ureg is a gtpase required for assembly of the nickel-containing active site of urease. herein, a strep-tagged klebsiella aerogenes ureg (ureg(str)) and selected site-directed variants of ureg(str) were constructed for studying the in vivo effects on urease activation in recombinant escherichia coli cells, characterizing properties of the purified proteins, and analysis of in vivo and in vitro protein-protein interactions. whereas the strep tag had no effect on ureg's ability to activate urease, ...201020533838
coliform accumulation in amphibalanus amphitrite (darwin, 1854) (cirripedia) and its use as an organic pollution bioindicator in the estuarine area of recife, pernambuco, brazil.samples of water and barnacles amphibalanus amphitrite were collected from recife, brazil, to assess if it accumulates total (tc) and thermotolerant coliforms (ttc) related with sewage pollution. the most probable number (mpn) values and the standard procedures for examination of shellfish were used. comparatively with the water samples, the highest coliform values came from the barnacles, with tc values ranging from < 3.0 x 10(3) to > 2.4 x 10(6) mpn.g-1, and ttc ranging from > 2.4 x 10(3) to 2 ...201020549061
bacterial coinfection and antimicrobial resistance in patients with paranasal sinus fungus balls.the aim of this study was to investigate the incidence of bacterial coinfection and antimicrobial resistance in cases of fungus balls of the paranasal sinuses.201020583740
the filter-feeding ciliates colpidium striatum and tetrahymena pyriformis display selective feeding behaviours in the presence of mixed, equally-sized, bacterial prey.this study examined whether two ciliates could discriminate between equally-sized bacterial prey in mixture and if so, how selectivity might benefit the ciliate population. live klebsiella aerogenes, k. ozaenae and escherichia coli, expressing different coloured fluorescent proteins, were cultured in such a way as to provide populations containing equally-sized cells (to prevent size-selective grazing taking place) and these prey were fed to each ciliate in 50:50 mixtures. colpidium striatum sel ...201020471910
quinazoline derivatives are efficient chemosensitizers of antibiotic activity in enterobacter aerogenes, klebsiella pneumoniae and pseudomonas aeruginosa resistant strains.amongst the three series of quinazoline derivatives synthesised and studied in this work, some molecules increase the antibiotic susceptibility of gram-negative bacteria presenting multidrug-resistant phenotypes. n-alkyl compounds induced an increase in the activity of chloramphenicol, nalidixic acid and sparfloxacin, which are substrates of the acrab-tolc and mexab-oprm efflux pumps in clinical isolates. these molecules are able to increase the intracellular concentration of chloramphenicol in ...201020494558
a nac for regulating metabolism: the nitrogen assimilation control protein (nac) from klebsiella pneumoniae.the nitrogen assimilation control protein (nac) is a lysr-type transcriptional regulator (lttr) that is made under conditions of nitrogen-limited growth. nac's synthesis is entirely dependent on phosphorylated ntrc from the two-component ntr system and requires the unusual sigma factor σ54 for transcription of the nac gene. nac activates the transcription of σ70-dependent genes whose products provide the cell with ammonia or glutamate. nac represses genes whose products use ammonia and also repr ...201020675498
[cloning of enterobacter aerogenes fh1a gene and overexpression of hydrogen production].we amplified and overexpressed the fhl activator (fh1a) in e. aerogenes atcc13408 to enhance hydrogen production.201020687337
physiological characterisation of the efflux pump system of antibiotic-susceptible and multidrug-resistant enterobacter aerogenes.enterobacter aerogenes predominates amongst enterobacteriaceae species that are increasingly reported as producers of extended-spectrum beta-lactamases. although this mechanism of resistance to beta-lactams is important, other mechanisms bestowing a multidrug-resistant (mdr) phenotype in this species are now well documented. amongst these mechanisms is the overexpression of efflux pumps that extrude structurally unrelated antibiotics prior to their reaching their targets. interestingly, although ...201020688487
identification and characterization of a phospholipase a2 from the venom of the saw-scaled viper: novel bactericidal and membrane damaging activities.phospholipase a(2) (pla(2)), a common toxic component of snake venom, has been implicated in various pharmacological effects. in this study, a basic myotoxic pla(2), named ectx-i was isolated from echis carinatus snake venom by using gel filtration on superdex g-75, and reverse phase hplc on c18 and c8 sepharose columns. pla(2), ectx-i was 13,861.72 molecular weight as estimated by maldi-tof (15 kd by sds-page), and consisted of 121 amino acid residues cross-linked by seven disulfide bonds. the ...201020723574
synergistic interaction of phenylpropanoids with antibiotics against bacteria.phenylpropanoids constitute a large part of our daily diet and there is a possibility that they might interact with synthetic drugs. the present work was aimed at studying the interaction of seven phenylpropanoids (cinnamic, p-coumaric, caffeic, chlorogenic, ferulic, 3,4-dimethoxycinnamic and 2,4,5-trimethoxycinnamic acid) with five antibiotics (amikacin, ampicillin, ciprofloxacin, erythromycin and vancomycin) against gram-negative (escherichia coli, enterobacter aerogenes and pseudomonas aerugi ...201020724513
in vitro ciprofloxacin resistance profiles among gram-negative bacteria isolated from clinical specimens in a teaching hospital.to evaluate the trends of ciprofloxacin resistance pattern in commonly isolated gram-negative bacteria over time in a saudi arabian teaching hospital.201020726190
microbial transformation of albendazole.screening scale studies were performed to biotransform anthelmintic drug albendazole by using twelve bacterial strains representing six genera and five actinomycetes cultures. among the cultures studied, bacillus subtilis mtcc 619, escherichia coli mtcc 118 and klebsiella pneumoniae mtcc 109 could transform albendazole to one metabolite whereas, enterobacter aerogenes ncim 2695, klebsiella aerogenes ncim 2258, pseudomonas aeruginosa ncim 2074 and streptomyces griseus ncim 2622 could transform al ...201020726341
genetics and regulation of the major enzymes of alanine synthesis in escherichia coli.genetic analysis of alanine synthesis in the model genetic organism escherichia coli has implicated avta, the still uncharacterized alaa and alab genes, and probably other genes. we identified alaa as yfbq. we then transferred mutations in several transaminase genes into a yfbq mutant and isolated a mutant that required alanine for optimal growth. for cells grown with carbon sources other than pyruvate, the major alanine-synthesizing transaminases are avta, yfbq (alaa), and yfdz (which we design ...201020729367
high-dose ciprofloxacin for serious gram-negative infection in an obese, critically ill patient receiving continuous venovenous hemodiafiltration.to describe the pharmacokinetic profile and clinical outcome associated with high-dose ciprofloxacin therapy in a patient with the triad of extreme obesity, multiple organ failure, and deep-seated infection.201020736424
isolation of inducible amp c beta-lactamase producing enterobacter aerogenes from a diabetic foot ulcer. 201021033310
trends in production of extended-spectrum beta-lactamases among enterobacteriaceae of clinical interest: results of a nationwide survey in belgian hospitals.to assess the frequency and diversity of extended-spectrum β-lactamases (esbls) in enterobacteriaceae isolates in belgium.201021036771
raman spectroscopy of xylitol uptake and metabolism in gram-positive and gram-negative bacteria.visible-wavelength raman spectroscopy was used to investigate the uptake and metabolism of the five-carbon sugar alcohol xylitol by gram-positive viridans group streptococcus and the two extensively used strains of gram-negative escherichia coli, e. coli c and e. coli k-12. e. coli c, but not e. coli k-12, contains a complete xylitol operon, and the viridans group streptococcus contains an incomplete xylitol operon used to metabolize the xylitol. raman spectra from xylitol-exposed viridans group ...201021037297
raman spectroscopy of xylitol uptake and metabolism in gram-positive and gram-negative bacteria.visible-wavelength raman spectroscopy was used to investigate the uptake and metabolism of the five-carbon sugar alcohol xylitol by gram-positive viridans group streptococcus and the two extensively used strains of gram-negative escherichia coli, e. coli c and e. coli k-12. e. coli c, but not e. coli k-12, contains a complete xylitol operon, and the viridans group streptococcus contains an incomplete xylitol operon used to metabolize the xylitol. raman spectra from xylitol-exposed viridans group ...201021037297
antibacterial activity of the latex of argemone ochroleuca sweet.to investigate the antibacterial effect of the crude latex of argemone ochroleuca (a. ochroleuca) as antibacterial potential against a range of human pathogenic bacteria.201021063649
virulence properties and integron-associated antibiotic resistance of klebsiella mobilis strains isolated from clinical specimens.this study examined klebsiella mobilis isolates cultured from clinical specimens for virulence-associated properties and antibiotic resistance. the strains produced a number of siderophores, including enterobactin, aerobactin and yersiniabactin. all isolates were able to adhere to and invade epithelial cells. they had cytotoxic activity, which caused destruction of human laryngeal epithelial hep-2 cells and evoked lysis of murine macrophage j774 cells. analyses of hep-2 and j774 cellular morphol ...201021088128
sers-based sandwich immunoassay using antibody coated magnetic nanoparticles for escherichia coli enumeration.a method combining immunomagnetic separation (ims) and surface-enhanced raman scattering (sers) was developed to enumerate escherichia coli (e. coli). gold-coated magnetic spherical nanoparticles were prepared by immobilizing biotin-labeled anti-e. coli antibodies onto avidin-coated magnetic nanoparticles and used in the separation and concentration of the e. coli cells. raman labels have been constructed using rod shaped gold nanoparticles coated with 5,5-dithiobis-(2-nitrobenzoic acid) (dtnb) ...201021125089
comparison of (gtg)5-oligonucleotide and ribosomal intergenic transcribed spacer (its)-pcr for molecular typing of klebsiella isolates.molecular typing of klebsiella species has become important for monitoring dissemination of β-lactamase-producers in hospital environments. the present study was designed to evaluate poly-trinucleotide (gtg)(5)- and rdna intergenic transcribed spacer (its)-pcr fingerprint analysis for typing of klebsiella pneumoniae and klebsiella oxytoca isolates. multiple displacement amplified dna derived from 19 k. pneumoniae (some with an esbl-phenotype), 35 k. oxytoca isolates, five k. pneumoniae, two k. o ...201021129413
prevalence of microorganisms of hygienic interest in an organized abattoir in mumbai, india.the magnitude of food-borne illnesses in india is unknown because of lack of surveillance networks. monitoring the prevalence of food-borne pathogens and indicators of contamination in primary production at abattoirs is imperative for creating a data bank and for effective control of such pathogens before they enter the food chain.201020818094
phytoremediation of mercury in pristine and crude oil contaminated soils: contributions of rhizobacteria and their host plants to mercury removal.the rhizospheric soils of three tested legume crops: broad beans (vicia faba), beans (phaseolus vulgaris) and pea (pisum sativum), and two nonlegume crops: cucumber (cucumis sativus) and tomato, (lycopersicon esculentum) contained considerable numbers (the magnitude of 10(5)g(-1) soil) of bacteria with the combined potential for hydrocarbon-utilization and mercury-resistance. sequencing of the 16s rrna coding genes of rhizobacteria associated with broad beans revealed that they were affiliated t ...201020833430
hexavalent chromium reduction by bacterial consortia and pure strains from an alkaline industrial effluent.to characterize the bacterial consortia and isolates selected for their role in hexavalent chromium removal by adsorption and reduction.201020854455
novel chalcones and 1,3,5-triphenyl-2-pyrazoline derivatives as antibacterial agents.novel sixteen chalcones and thirteen 1,3,5-triphenyl-2-pyrazolines were synthesized and characterized using ft-ir, hr-mass, nmr (¹h-nmr, ¹³c-nmr, 135 dept, ¹h-¹h cosy and ¹h and ¹³c cosy) and xrd. these compounds were evaluated for their antibacterial activity against six micro-organisms, namely bacillus subtilis ncim 2718, staphylococcus aureus ncim 5021, salmonella typhi ncim 2501, enterobacter aerogenes ncim 5139, pseudomonas aeruginosa ncim 5029, and proteus vulgaris ncim 2813 by twofold dil ...201020925692
aerobic growth at nanomolar oxygen concentrations.molecular oxygen (o(2)) is the second most abundant gas in the earth's atmosphere, but in many natural environments, its concentration is reduced to low or even undetectable levels. although low-oxygen-adapted organisms define the ecology of low-oxygen environments, their capabilities are not fully known. these capabilities also provide a framework for reconstructing a critical period in the history of life, because low, but not negligible, atmospheric oxygen levels could have persisted before t ...201020974919
an outbreak of klebsiella pneumoniae and enterobacter aerogenes bacteremia after interventional pain management procedures, new york city, 2008.in october 2008, an investigation was conducted into a cluster of gram-negative bloodstream infections after invasive pain management procedures at an outpatient facility to identify additional cases and determine the source of illness.201020975462
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