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gastroenteritis causing critical limb ischaemia.six days after admission to hospital with salmonella gastroenteritis, this patient presented with a critically ischaemic leg, having developed an iliac occlusion, and a subcutaneous salmonella abscess in the anterior compartment of the leg. critical limb ischaemia and abscess formation can be added to infective aortic aneurysm as vascular complications of salmonella gastroenteritis.19969015921
[the pathogenetic and clinico-diagnostic significance of changes in leukocyte migration activity in patients with shigellosis and salmonellosis].the peripheral blood leukocyte migration activity (lma) was studied in 134 acute dysentery patients and 129 salmonellosis patients by using the in vitro screening assay of cell migration (sacm) from microcultures, stimulated with lps antigens from a number of enteric pathogens. at the acute period of the disease (days 1-5) in the greater part of the patients (80-90%) the specific acceleration of lma, later followed by its inhibition, was registered in the presence of the pronounced intoxication ...19969027172
[salmonellosis in moscow: its epidemiological characteristics and the prevention tasks].zooanthroponotic salmonella infections in moscow are characterized, on the whole, by the same epidemiological characteristics as in other areas of russia. despite the pronounced polyetiological character of these infections, only a few salmonella serovars were found to dominate in their dynamics for many years. the 1970s were characterized by a growth in morbidity due to a wide spread of s.typhimurium among children of early age in hospitals. this situation was stabilized by 1985, but since 1986 ...19969027175
[epidemiological data on salmonellosis due to salmonella enteritidis in some areas of the russian federation].a rise in morbidity caused by s. enteritidis at individual territories of the russian federation in the second half of 1980s was due to the consumption of insufficiently heated infected chicken eggs and the nonobservance of sanitary and hygienic rules in the preparation of food from chicken meat. the spread of s. enteritidis in the russian federation occurred mainly at the territories supplied with incubator eggs from the same poultry-breeding enterprise. s. enteritidis strains isolated from inf ...19969027177
salmonella enteritidis outbreak in thailand: study by random amplified polymorphic dna (rapd) analysis.an outbreak of salmonella enteritidis in thailand was reported in 1990. the majority of isolates were found in chicken and human throughout the country. the continuation of a high rate of spreading which is presently continuing prompted us to investigate possible clonal involvement in the outbreak. one hundred and twenty five isolates of s. enteritidis which were isolated between 1990-1993 were clonally identified by the technique of random amplified polymorphic dna (rapd) analysis. eight profil ...19969031413
[osteomyelitis by salmonella enteritidis and sickle cell hemoglobinopathy. report of a case and review of the literature].salmonella osteomyelitis is an uncommon disease, usually associated with sickle cell anemia and other hemoglobinopathies, as well as with other disease states. in this case, osteomyelites was apparently caused by hematogenous spread of an enteric infection by salmonella enteritidis. bone involvement, in vertebral bodies, was resolved after prolonged clinical treatment with antibiotics. we discuss the pathogeny and compare the findings of four case with others related in literature.19969035499
[antibiotic resistance in salmonella enterica: an increasing problem].to determine the evolution of the frequencies of salmonella enterica serotypes and their resistance to antimicrobial agents.19969035708
plasmid characterization and pulsed-field electrophoretic analysis demonstrate that ampicillin-resistant strains of salmonella enteritidis phage type 6a are derived from salm. enteritidis phage type 4.plasmid incompatibility studies have demonstrated that strains of salmonella enteritidis phage type (pt) 6a resistant to ampicillin possess a 36 megadalton incompatibility group (inc) x plasmid coding for resistance to ampicillin which is capable of converting strains of salm. enteritidis belonging to pts 1 and 4 to pt 6a, and pt 8 to pt 13. however, pulsed-field gel electrophoresis (pfge) has demonstrated that all clinical isolates of pt 6a have a characteristic xbai pulsed-field profile which ...19968972088
a rat model of infection by salmonella typhimurium or salm. enteritidis.salmonellosis in the rat has many similarities with the disease in humans, with the ileum thought to be the main site of colonization/invasion in both species. thus, the rat may be a useful way to study the mechanism of infection by these pathogenic bacteria. a series of infection trials carried out with hooded lister rats showed that a salmonella infection persisted for an extended period of time and that salmonellae bind to the small intestinal epithelium as early as 4 h after intragastric int ...19968972092
intestinal colonization and organ invasion in chicks experimentally infected with salmonella enteritidis phage type 4 and other phage types isolated from poultry in the united states.phage type 4 salmonella enteritidis strains have only recently been isolated from poultry and humans in the united states, although this phage type predominates in many other countries. the present study assessed the ability of s. enteritidis isolates of various phage types found in the united states (including phage type 4) to colonize the intestinal tract and invade to reach internal organs in experimentally infected chicks. groups of 5-day-old single-comb white leghorn chicks were inoculated ...19968980817
an epidemiological study of salmonella enteritidis by pulsed-field gel electrophoresis (pfge): several pfge patterns observed in isolates from a food poisoning outbreak.an epidemiological analysis of salmonella enteritidis from a food poisoning was done using pulsed-field gel electrophoresis (pfge) of blni- or xbai-digested fragments of chromosomal dna of isolates. s. enteritidis isolates obtained from 19 patients had identical pfge patterns. therefore, a strain giving the same pattern was considered to be the causative agent of this outbreak. in addition, four isolates that had different blni-digested pfge patterns were obtained from three patients, suggesting ...19968985943
antagonistic effects of intestinal lactobacillus isolates on pathogens of chicken.twelve lactobacillus strains isolated from chicken intestine, which demonstrated a strong and moderate capacity to adhere to the ileal epithelial cells in vitro, were used to investigate their inhibitory ability against five strains of salmonella, i.e. salmonella enteritidis 935/79, salm. pullorum, salm. typhimurium, salm. blockley and salm. enteritidis 94/448, and three serotypes of escherichia coli, viz. e. coli o1:k1, o2:k1 and o78:k80. the results showed that all the 12 lactobacillus isolate ...19968987444
survival of salmonella enteritidis pt4 and salm. typhimurium swindon in aerosols.small particle aerosols of plate-grown salmonella enteritidis and salm. typhimurium were generated and maintained within a rotating drum at 75% relative humidity and 24 degrees c for 2 h. plate-grown organisms were found to be more aerosol-stable than broth-grown organisms. differences were observed between the two species; plate-grown salm. typhimurium retained 100% viability after 2 h compared to approximately 70% for plate-grown salm. enteritidis. a large proportion of cells of both serotypes ...19968987450
growth/survival of salmonella enteritidis on fresh poultry and fish stored under vacuum or modified atmosphere.the effect of vacuum and modified atmosphere packaging on the growth/survival of salmonella enteritidis on fresh poultry and fish (boops boops) is described. salmonella enteritidis survived but did not grow significantly in all samples (poultry or fish) at 3 degrees c. at 10 degrees c the numbers of salm. enteritidis increases rapidly in vacuum-packed samples and in samples flushed with 100% n2, 20% co2/80% o2 of both types of proteinaceous food. growth was also evident in fish and poultry flush ...19968987452
cross-infection of chicks by airborne transmission of salmonella enteritidis pt4.airborne cross-infection by salmonella enteritidis pt4 strains was demonstrated between sets of orally infected 1-d-old chicks and identical uninfected control chicks. low numbers of salm. enteritidis were detected in the air of the rooms housing the chicks. sentinel mice within the rooms did not become infected. this study demonstrates that low levels of airborne salm. enteritidis are a potential source of cross-infection in poultry houses.19968987717
risk factors for outbreaks of infectious intestinal disease linked to domestic catering.the epidemiology of general outbreaks of infectious intestinal disease associated with domestic catering for large numbers is described and compared with foodborne outbreaks in other settings. from 1 january 1992 to 31 december 1994, the phls communicable disease surveillance centre identified 101 foodborne general outbreaks of infectious intestinal disease associated with domestic catering in england and wales (16% of all foodborne outbreaks). salmonella species were associated with 77 of the 1 ...19968990573
marshmallows cause an outbreak of infection with salmonella enteritidis phage type 4.thirty-six cases of salmonella enteritidis phage type (pt) 4 infection, mainly in children, were notified in one local authority in the two weeks beginning on 23 october 1995. twenty-four cases and 42 controls nominated by cases were included in a case control study, which showed a significant association between illness and the consumption of marshmallow confectionery from a bakery. s. enteritidis pt4 was isolated from samples of the marshmallow, and all isolates had the same plasmid and pulsed ...19968990574
food poisoning among clients of a meals on wheels service.sixty-one out of 105 elderly people who received a meal from a meals on wheels service developed a diarrhoeal illness. twenty-seven of the 61 were admitted to hospital and three died. epidemiological evidence suggested that food poisoning was associated with eating strawberry mousse made with raw eggs. salmonella enteritidis phage type 4 was identified as the causative organism.19968990575
[septic and other forms of salmonellosis]. 19968992102
decision criteria for vaccination against salmonella in poultry. 19968996890
the mechanism of bactericidal action of normal human serum against salmonella rods.forty salmonella strains sensitive to the bactericidal action of serum were investigated. all these strains were susceptible to complement activated by the classical pathway though in part (60%) of these strains the presence of lysozyme was necessary for killing. s. typhimurium rods were susceptible to only one mechanism of the action of bactericidal factors. on the contrary, s. enteritidis strains were sensitive to three various mechanisms of bactericidal action of serum. next eight forms of sa ...19968997694
phage types and plasmid profiles of salmonella enteritidis strains.112 strains of salmonella enteritidis were tested according to phage typing method and plasmid profile analysis. 66 strains were classified as pt4, 29 strains as pt6 and 17 strains as pt10. the strains were differentiated into 17 groups according to plasmid profiles.19968997698
acute hematogenous osteomyelitis of a closed fracture with chronic superinfection.acute hematogenous salmonella osteomyelitis is rare among immunocompetent adults. in this study, the authors reported an unusual case of salmonella enteriditis osteomyelitis of the humerus complicated by methicillin-resistant staphylococcus aureus superinfection and eventual chronic osteomyelitis in an immunocompetent host. resection of the humeral head and a significant portion of the humeral shaft coupled with numerous surgical debridements and intravenously administered antibiotics led to res ...19968998882
[immunization with potential salmonella enteritidis mutants-- 2. investigations on the attenuation and immunogenicity for mice and young hens].adenine-amino acid auxotrophic salmonella enteritidis double mutants were investigated for their in vivo stability, attenuation und immunogenicity using nmri-mice and spf-chicks. all mutants used were stable in vivo and attenuated. two mutants were selected for immunization experiments in chickens according to their protective ability after a single intraperitoneal immunization in nmri-mice using a homologous and heterologous challenge. after two oral immunizations and homologous oral challenge ...19968999767
[sanitation of biowaste compost by using salmonella enteritidis as a pathogen indicator and survival of salmonella in seepage water].the following investigations show the frequency of the occurrence of salmonella in biowastes. salmonellae, belonging to the serotypes salmonella enteritidis, infantis and agona were detected in 50% of the investigated biowaste samples. in order to control the reliability of compositing techniques on eliminating pathogens, salmonella enteritidis was used as a pathogen indicator, which was brought into a container composting process and investigated on it's decline. although the input indicator ba ...19968999779
screening of in vitro antibacterial activity from syzygium guineense (willd) hydrosoluble dry extract.diarrhoea is one of the most important causes of infant mortality in the world. as modern drugs are expensive or unavailable in developing countries, many people use traditional medicines in africa for the treatment of several diseases. in our study, we investigated the antibacterial activity of syzygium guineense extract in order to assess its activity on some bacterial strains involved in diarrhoeal diseases and to justify its use. the aqueous dry extract was prepared by decoction followed by ...19969008903
variations of invasive salmonella infection in elderly people.four cases of elderly people with differing forms of invasive salmonella infection are presented. vulnerability factors and patterns of presentation are discussed.19969039721
the first salmonella enteritidis phage type 1 infection of a commercial layer flock in finland.the first salmonella enteritidis phage type (pt) 1 infection in a commercial layer flock of 2700 birds in finland occurred in 1995. all the birds were ordered to be killed, the eggs to be destroyed and access to the layer house was denied in order to prevent spread of the infection. ninety one commercial layers, 61 replacement pullets and 1062 eggs were collected for the analyses. the total infection level of the flock was 8%, concentrated on the 2 older age groups. s. enteritidis pt1 was isolat ...19969050280
[epidemiology of gastroenteritis by salmonella (1983-1994)].the incidence of salmonella enteritidis infection has been study in order to determine their epidemic spread from 1983 to 1994, and the impact of the measures introduced to attempt its control.19969053003
characterisation of monoclonal antibodies specific to sef 21 fimbriae of salmonella enteritidis and their reactivity with other salmonellae and enterobacteria.a panel of monoclonal antibodies (mabs) specific to type 1 (sef 2) fimbriae of s. enteritidis was produced using crude and hplc purified preparations of sef 21 fimbriae. sixteen mabs were selected by indirect elisa using both purified sef 21 antigen and whole cells of s. enteritidis. eight mabs were confirmed by immunoprecipitation assay to react specifically with sef 21 fimbriae. these mabs were further characterised for their reactivity patterns by the "whole cell" elisa and latex agglutinatio ...19969054118
[immunization with potential salmonella enteritidis mutants--1. production and in vitro characterization].production and in vitro characterization of potential vaccine strains are the first steps leading to an efficient salmonella enteritidis oral live vaccine for homologous immunization of poultry. the paper presents the results of the production of adenine-amino acid auxotrophic mutants using n-methyl-n'-nitro-n-nitrosoguanidine mutagenesis. the mutant strains were characterized using the following properties: auxotrophy, stability of mutation, reversion rate, generation time, metabolic properties ...19969054331
[the enterosorption treatment of patients with acute intestinal infections and chronic colitis with diarrhea].a total of 234 patients with acute intestinal infections (aii) and chronic colitis presenting with diarrhoea were examined. in an acute phase of aii induced by conditionally pathogenic flora, salmonellae and shigellae, as well as in exacerbation of chronic colitis, blood aggregability appears to be on the increase, with the ability of erythrocytes to deformation getting worse. in rectal mucosa hemodynamic disorders are common, manifested by decrease in pulse blood filling, rate of bloodflow pred ...19969072235
[a small outbreak of salmonellosis due to salmonella enteritidis]. 19969103064
comparative study of the protective effect against salmonella colonisation in newly hatched spf chickens using live, attenuated salmonella vaccine strains, wild-type salmonella strains or a competitive exclusion product.there is a need to prevent intestinal colonisation by salmonella enteritidis and s. typhimurium in newly hatched chicks. treatment with an undefined bacterial flora is not acceptable to regulatory agencies in some countries because of the potential risk of transmitting pathogens. a defined culture with a potency and stability equivalent to those of an undefined culture has not yet been developed. since attenuated salmonella vaccine strains could possess the colonisation characteristics but not t ...19979105931
effect of feed withdrawal on the incidence of salmonella in the crops and ceca of market age broiler chickens.previous research regarding salmonella contamination in poultry has focused predominantly on cecal and intestinal contamination. recently, the crop has been implicated as an important source of carcass contamination within the processing plant. in the present study, broiler chickens were orally challenged with 1 x 10(8) cfu s. enteritidis at 6 wk of age. at 7 wk of age, birds were randomly divided into two groups consisting of full access to feed, or total feed withdrawal, 18 h prior to sample c ...19979106896
impact of infecting dose on severity of disease in an outbreak of food-borne salmonella enteritidis.we conducted a cross-sectional study 72 h after a private dinner party, at which 33/59 participants (attack rate 56%) became infected with salmonella enteritidis, phage type 6. all were interviewed and stool samples were obtained from 55 persons (93%). only 2/33 cases were asymptomatic (6%). the median incubation period was 21 h. 14 of the cases (42%) were admitted to hospital. a significant association was found between infection and 1 of the egg-containing dishes (kabab). cross-contamination t ...19979112296
salmonella enteritidis colonization in turkey poults.an experimental trial on the colonization of two salmonella enteritidis strains (phage types 4 and 8) originating from meat turkey flocks, were carried out. three-day old poults were inoculated orally with approximately 10(6) cfu/bird (one group with pt8 and the other with pt4). clinical signs were not observed in any of the groups. the total reisolation rates from cloacal swabs in birds inoculated with pt4 was higher than from birds inoculated with pt8. the inoculated strains could also be dete ...19979112801
enterotoxin and cytotoxin production by salmonella enteritidis strains isolated from gastroenteritis outbreaks.seventy-six salmonella enteritidis, three salmonella virchow and one salmonella bradenrup strains were screened for enterotoxigenicity by using the chinese hamster ovary (cho), y1 adrenal, vero and hela cell tests. all the strains gave positive reactions for enterotoxin production, except one, and the relative sensitivity to the toxin exhibited by the different cell lines was evaluated. an enterotoxic activity has been identified in sonicated extracts of salm. enteritidis. this enterotoxin was p ...19979113874
rapid detection of salmonella enterica with primers specific for irob.the irob gene of salmonella enterica is absent from the chromosome of the related organism escherichia coli. we determined the distribution of this gene among 150 bacterial isolates, representing 51 serotypes of different salmonella species and subspecies and 8 other bacterial species which are frequent contaminants during routine enrichment procedures by southern hybridization. an irob-specific dna probe detected homologous sequences in all strains of s. enterica, including serotypes of s. ente ...19979114411
effects of eicosapentaenoic and gamma-linolenic acid on lung permeability and alveolar macrophage eicosanoid synthesis in endotoxic rats.proinflammatory eicosanoids (cyclooxgenase and lipoxygenase metabolites of arachidonic acid) released by alveolar macrophages play an important role in endotoxin-induced acute lung injury. we investigated the effect of prefeeding rats for 21 days with enteral diets that provided the anti-inflammatory fatty acids, eicosapentaenoic acid and gamma-linolenic acid (derived from fish oil and borage oil, respectively), as compared with an n-6 fatty acid-enriched diet (corn oil) on the following: a) lun ...19979118672
effects of inhibition of nitric oxide synthase by aminoguanidine in acute endotoxemia.nitric oxide (no) has been implicated in the pathogenesis of the circulatory dysfunction of endotoxin shock. we investigated the effect of aminoguanidine (ag), an inhibitor of nitric oxide synthase (nos) that is more selective for the inducible nos, on the circulatory and inflammatory sequelae after administration of a bolus (10 mg/kg iv) of lipopolysaccharide (lps) (salmonella enteritidis). rats receiving lps + vehicle (lps + veh) exhibited a 73% decrease in mean arterial blood pressure (mabp) ...19979124447
in vivo production and function of il-12 p40 homodimers.the bioactivity of il-12 is mediated by heterodimers of disulfide-linked p35 and p40 protein subunits. homodimeric p40 competes with heterodimer for binding to the high affinity il-12r and inhibits il-12 bioactivity in vitro. however, the production and significance of p40 homodimer as a cytokine antagonist in vivo have not been determined. in these studies, we observed increased amounts of both il-12 p40 monomer and homodimer in the serum of c57bl/6 mice following injection of 300 microg of sal ...19979127002
correlation of susceptibility and resistance of twenty-five bacterial strains by analysis of mic database of cephalosporins and oxacephalosporins.mic database of 1,407 cephalosporins and oxacephalosporins was utilized to characterize 25 bacterial strains including gram-positive and gram-negative bacteria. mic values were converted to activity rank indices, and distribution patterns of these indices were compared among the bacterial strains. salmonella enteritidis. no. 11 was the most susceptible bacterium, and the activity against this strain was ascribed to the binding of beta-lactams to the pbps without any significant barrier to the ap ...19979127196
salmonella enteritidis in an endometriotic ovarian cyst.isolated salmonella infections are rare. in view of the rarity, we present a case of a 16-year-old patient with an endometriotic ovarian cyst where salmonella enteritidis was isolated from aspirated material from the cyst. we include a review of the world literature on ovarian salmonella infection from 1966 to june 1996.19979134404
the cross-contamination and survival of salmonella enteritidis pt4 on sterile and non-sterile foodstuffs.the ability of two strains of salmonella enteritidis pt4 to cross-contaminate from inoculated egg droplets on surfaces onto melon or beef (sterile or non-sterile) was investigated. when the foods were placed on these surfaces where egg droplets were still wet, cross-contamination occurred within 1 s onto every piece of food. it took at least 1 min for all the food pieces to be contaminated when egg droplets had been allowed to dry. both strains were capable of rapid growth on melon and beef (ste ...19979134772
[the effect of hiv infection on high incidence of heart diseases in kinshasa (zaire). echocardiographic study].invasion of the heart by hiv has become a clinical problem over the last decade. the objective of the present study was to systematically detect the excess hiv-related cardiac lesions in kinshasa by performing echocardiography. the study population consisted of 166 hiv-infected patients and 166 hiv-seronegative patients with heart disease (control group). 69% of patients were at stage a of hiv infection and 31% were at stage aids c3 according to cdc 1993 criteria. a higher incidence of echocardi ...19979137674
salmonella osteomyelitis in sickle cell disease children in the east end of london.five cases of salmonella osteomyelitis are presented in children with sickle cell disease living in the east end of london. four of these had involvement of the long bones and one (an infant) presented with hand-foot syndrome. four required surgical drainage of pus and all required lengthy therapy with antibiotics. factors contributing to the increased susceptibility of these patients to invasive disease with salmonella sp. are discussed as are the clinical features, surgical and antibiotic mana ...19979138136
induction of flagellation and a novel agar-penetrating flagellar structure in salmonella enterica grown on solid media: possible consequences for serological identification.salmonella enterica grown on solid medium containing iron, thiosulfate and 100 mm hexoses and amino acids underwent cell surface differentiation involving increased flagellation (electrophoretic isotypes 60, 54 and 50 kda), conversion from rough to smooth lipopolysaccharide, and assembly of a matrix that penetrated 1.4% agar. flagellation was also induced in the avian pathogen s. enterica var pullorum, which is diagnostically defined as aflagellate. induction correlated closely with a simple col ...19979141658
homologous regions of the salmonella enteritidis virulence plasmid and the chromosome of salmonella typhi encode thiol: disulphide oxidoreductases belonging to the dsba thioredoxin family.the nucleotide sequence relatedness between the chromosome of salmonella typhi and the virulence plasmid of salmonella enteritidis was investigated using short dna probes of < 2 kb covering the whole virulence plasmid sequence. only one homologous region was detected. this region was subsequently cloned and partially sequenced. sequences closely related to the pefl gene and the orfs orf7, orf8 and orf9, which are located downstream of the fimbrial pef operon of the salmonella typhimurium virulen ...19979141703
a virulent isolate of salmonella enteritidis produces a salmonella typhi-like lipopolysaccharide.the lipopolysaccharide (lps) of salmonella enteritidis has been implicated as a virulence factor of this organism. therefore, the lps from a stable virulent isolate, se6-e21, was compared with that from an avirulent isolate, se6-e5. the lpss were extracted, and the high-molecular-weight (hmw) lps was separated from the low-molecular-weight (lmw) lps for both isolates. both the hmw and lmw lpss were characterized by glycosyl composition and linkage analyses. immunochemical characterization was pe ...19979079895
genomic analyses of salmonella enteritidis phage type 4 strains from austria and phage type 8 strains from the united states.forty illness associated phage-type (pt) 4 and pt 8 strains of salmonella enteritidis were analyzed by the pulsed-field technique of clamped homogeneous electric fields (chef) electrophoresis. using noti and xbai, the 40 strains were subdivided by each enzyme into seven restriction endonuclease digestion profiles (redp). the 35 pt 4 isolates from austria were subdivided into six noti and five xbai redp, while the five pt 8 isolates from the united states displayed a single noti and two xbai redp ...19979084111
occurrence of salmonella enterica serotype enteritidis phage types in the slovak republic.phage typing of 741 isolates of salmonella enterica serotype enteritidis from the slovak republic in 1995 has been carried out using the scheme of ward and colleagues (1987). 202 strains (51 isolated from food) were from 9 outbreaks, 536 isolates were from sporadic cases and 3 isolates were from nosocomial infections of new-born babies. 704 isolates (95%) from all sources were typeable and belonged to 7 different phage types (pts). pt8 was the phage type most frequently identified (72.6%). other ...19979085013
effect of inv mutations on salmonella virulence and colonization in 1-day-old white leghorn chicks.invasion of salmonella into the cells of the intestinal epithelium is an important step in the infection process. this initial invasion is followed by colonization of other organs throughout the body. in an attempt to better understand this process, we moved defined mutations in several genes of the inv locus into salmonella typhimurium uk-1 and two strains of salmonella enteritidis. these mutant strains were evaluated for their oral and intraperitoneal virulence as determined by 50% lethal dose ...19979087319
destruction of salmonella enteritidis in poultry feed by combination of heat and propionic acid.a factorial laboratory experiment was conducted to assess the effects of heating times of 0, 20, 40, and 80 sec at 160 f and propionic acid concentrations of 0, 0.1%, and 0.2% on reduction of salmonella enteritidis in poultry feed with approximately 15% moisture. the results showed that after 80 sec heating time an approximately 10,000-fold reduction in living salmonella was obtained in the samples with 0.2% propionic acid. survival in the 0.2% acid group was 2 log10 lower than in the 0.1% and c ...19979087320
the effects of induced molting on the severity of acute intestinal inflammation caused by salmonella enteritidis.this study describes and compares early inflammation caused by salmonella enteritidis in molted and nonmolted hens. adult white leghorn chickens were orally infected with salmonella enteritidis 4 days after feed removal. at 2, 4, 8, 10, 24, 48, 72, and 96 hr after infection, the hens were euthanatized, and the duodenum, jejunum, ileum, cecum, and colon were evaluated by light microscopy. two trials were conducted, and in both trials inflammation occurred more frequently and was significantly gre ...19979087328
efficacy of two avian salmonella-immune lymphokines against liver invasion in chickens by salmonella serovars with different o-group antigens.newly hatched chicks are susceptible to organ invasion by various serovars of salmonella. we have previously shown that intraperitoneal administration of salmonella enteritidis-immune lymphokine (seilk) increased resistance to organ invasion by s. enteritidis (se) in day-of-hatch chicks. in the present study, we determined that salmonella-immune lymphokines can provide protection against organ invasion by serovars that are unrelated to the original serovar used as an immunogen. immune lymphokine ...19979087335
applying tests for specific yolk antibodies to predict contamination by salmonella enteritidis in eggs from experimentally infected laying hens.detecting salmonella enteritidis contamination in eggs has become the cornerstone of many programs for reducing egg-borne disease transmission, but egg culturing is time consuming and laborious. preliminary screening tests are thus generally applied to minimize the number of flocks from which eggs must be cultured. the usefulness of such tests is directly proportional to both their detection sensitivity and their ability to predict the likelihood of egg contamination. in the present study, sampl ...19979087337
antibacterial activity of formic and propionic acids in the diet of hens on salmonellas in the crop.1. the inclusion of formic and propionic acids in the form of bio-add to the food of hens made no difference to the ph of the intestinal tract, but resulted in higher concentrations of these acids in the contents of the crop and gizzard. 2. organic acids in the crop contents were bactericidal for salmonella serotype enteritidis pt4 in vitro, and also caused sub-lethal damage because fewer cells were recovered on selective salmonella media (brilliant green phenol red agar) than on non-selective m ...19979088614
on-farm monitoring of mouse-invasive salmonella enterica serovar enteritidis and a model for its association with the production of contaminated eggs.mice (mus musculus) captured in henhouses were assessed for the presence of salmonellae in spleens. of 621 and 526 spleens cultured during the first and second years of collection, 25.0 and 17.9%, respectively, were positive for salmonella enterica serovar enteritidis. contaminated eggs were cultured from nine houses during the first year of sampling, and for eight of these houses, serovar enteritidis was recovered from the spleens of mice. rank sum statistical analysis of positive mouse spleens ...19979097453
genetic map of the virulence plasmid of salmonella enteritidis and nucleotide sequence of its replicons.partial sequencing of a genomic library of the virulence plasmid of salmonella enteritidis has been used to localize in the restriction map of this plasmid the genetic loci already described in other salmonella plasmids. the comparison of the vestigial tra region with the corresponding genes in the f plasmid allowed us to define the extent of the deletions that the s. enteritidis plasmid should have suffered. the putative replicons of the plasmid, repb and repc, were isolated and both proved to ...19979099859
evaluation of an aroa mutant salmonella typhimurium vaccine in chickens using modified semisolid rappaport vassiliadis medium to monitor faecal shedding.in groups of chickens vaccinated orally or intramuscularly with a live aroa mutant salmonella typhimurium vaccine strain and then experimentally inoculated with 10(8) cfu of wild type s. typhimurium or 10(9) cfu of s. enteritidis, faecal shedding of the vaccine and wild type strains was monitored by the buffered peptone water-modified semisolid rappaport vassiliadis medium method, which detected less than 10(2) cfu per gram of faeces. the vaccine strain was shed in the faeces for up to 26 days. ...19979100326
septicemia caused by salmonella infection: an overlooked complication of sickle cell disease.to describe the characteristics of salmonella infections in sickle cell disease and to compare the features of osteomyelitis and those with bacteremia/septicemia without obvious bone changes. to search for risk factors for osteomyelitis, and to draw attention to the frequency and significance of salmonella bacteremia/septicemia.19979063414
transient radiological and colonoscopic features of inflammatory bowel disease in a patient with severe salmonella gastroenteritis.salmonella is the most commonly reported cause of food-borne outbreaks of gastroenteritis. we report a case of a severe and toxic form of enteritis caused by salmonella enteritidis. findings of colonoscopy, an upper g1 tract series, and small bowel follow-through were consistent with those of inflammatory bowel disease, but the enteritis was self-limited, and the patient recovered after supportive treatment only and has remained well.19979040224
yield from stool testing of pediatric inpatients.to quantify the yield from stool testing in pediatric inpatients and to identify criteria to test stool more deliberately without sacrificing diagnostic sensitivity.19979041868
novel transferable beta-lactam resistance with cephalosporinase characteristics in salmonella enteritidis.an isolate of salmonella enteritidis was found to produce a plasmid-encoded beta-lactamase (dha-1) that conferred resistance to extended-spectrum cephalosporins and cephamycins. the substrate and inhibition profiles of this enzyme resemble a class c beta-lactamase. this is the first report of a plasmid-mediated cephalosporinase of this class in the salmonella genus.19979044032
role of sefa subunit protein of sef14 fimbriae in the pathogenesis of salmonella enterica serovar enteritidis.in this study, the role of the sefa subunit protein of sef14 fimbriae in the pathogenesis of salmonella enterica serovar enteritidis was investigated. this was accomplished by mutating the sefa gene in the chromosome of two strains of s. enterica serovar enteritidis by allelic exchange with a copy that has been inactivated by interruption with a nonpolar kanamycin resistance (apha-3) cassette. the effect of this mutation on the ability of the s. enterica serovar enteritidis strains to colonize t ...19979009334
detection of the induction of salmonella enterotoxin gene expression by contact with epithelial cells with rt-pcr.all strains of salmonella enterica investigated were found to carry the salmonella enterotoxin gene (stn) as determined by pcr and hybridization studies. however, when using cho-k1 cells for testing the toxicity of the strains, not all strains showed a toxic effect (cell elongation) on the cells or did so only at a low level. the cultivation of salmonella in contact with epithelial cells (iec-6) led to an increase in the production of toxin. the stn gene expression was detectable with the help o ...19979011037
d values of salmonella enteritidis isolates and quality attributes of shell eggs and liquid whole eggs treated with irradiation.irradiation sensitivity of five salmonella enteritidis isolates inoculated either on the surface or inside of whole shell eggs were determined. the shell eggs were irradiated at doses of 0, 0.5, 1.0, and 1.5 kgy. a minimal dose of 0.5 kgy was sufficient to eliminate all the isolates from the surface of whole eggs; however, the same isolates were more resistant to irradiation when present inside the eggs. the atcc 13076 isolate was significantly more sensitive to irradiation, with a d value of 0. ...19979037706
salmonella spondylitis.we report a case of salmonella spondylitis in an adolescent without sickle cell disease or any history of salmonella gastroenteritis. the infecting organism (cultured from material aspirated from the bone lesion) was salmonella enterica serovar newport. with nonoperative treatment, evolution was favorable.19979039673
osteomyelitis of the spine due to salmonella infection--conservative treatment with quinolone: a case report.although osteomyelitis due to salmonella infection is known to be associated with sickle cell anemia, various hemoglobinopathies and immune suppressive states, it may also occur in normal hosts. a 16-year-old chinese boy without sickle cell disease or any other condition that would compromise the immune system had osteomyelitis of the lumbar spine caused by salmonella enteritidis. the condition was treated conservatively with ciprofloxacin (quinolone group). this may be the first reported case i ...19979030084
analysis of whole-cell fatty acid profiles of verotoxigenic escherichia coli and salmonella enteritidis with the microbial identification system.differentiation of strains within bacterial species, based on gas chromatographic analysis of whole-cell fatty acid profiles, was assessed with 115 strains of verotoxigenic escherichia coli and 315 strains of salmonella enteritidis. fatty acid-based subgroups within each of the two species were generated. variability of fatty acid profiles observed in repeat preparations from the same strain approached that observed between subgroups, limiting the usefulness of using fatty acid profiles to subgr ...19979023953
correlation of conversion of salmonella enterica serovar enteritidis phage type 1, 4, or 6 to phage type 7 with loss of lipopolysaccharide.studies of pairs of salmonella enterica serovar enteritidis isolates from different poultry flocks showed that phage type (pt) 7 may be derived from pt 1, 4, and 6. the conversion appeared to be associated with loss of lipopolysaccharide. it is concluded that pt 7 may be of little value as an epidemiological marker of s. enterica serovar enteritidis.19978968942
superficial salmonella abscesses in two siblings with sickle cell diseases.salmonella infection in sickle cell disease patients is generally well-known but presentation as superficial abscess is relatively uncommon. two sisters aged 4 1/2 and 6 years presented with superficial subcutaneous abscesses that were caused by the same strain of salmonella enteriditis group c. despite in vitro susceptibility with a mic of 0.03 microgram/ml and an adequate dosage of ciprofloxacin there was a relapse with widespread dissemination of the same organism in the younger sister who su ...19979329291
bacteriological monitoring of salmonella enteritidis carrier birds after decontamination using enrofloxacin, competitive exclusion and movement of birds.two hundred and forty, four-week-old laying birds naturally infected with salmonella enteritidis pt33 (pasteur institute phage typing system) were randomly divided twice (before and during the treatments) to obtain four separately housed groups of 60 birds and to study the efficacy of three decontamination treatments: enrofloxacin either with or without the movement of birds to a clean area, and enrofloxacin combined with movement of birds and a competitive exclusion treatment. the control group ...19979330473
[salmonellosis in 1995]. 19979333866
crucial role of interleukin-10/interleukin-12 balance in the regulation of the type 2 t helper cytokine response in reactive arthritis.to investigate whether a predominant type 1 t helper (th1) or th2 cytokine pattern is present in the joints of patients with reactive arthritis (rea), and whether the cytokine pattern can be modulated by cytokines or anticytokines.19979336412
pharmacodynamic parameters of ofloxacin, tobramycin and ceftriaxone and their effect on the biological properties of salmonellae.the postantibiotic effect and postantibiotic sub-mic effect of ofloxacin (cas 82419-36-1), tobramycin (cas 32986-56-4) and ceftriaxone (cas 73384-59-5) on two salmonella serotypes (s. typhimurium and s. enteritidis) were studied. the influence of postantibiotic effect and postantibiotic sub-mic effect of the antibiotics on prophage induction of the lysogenic s. typhimurium strain and on congo red binding by both serovars as indicator of their invasive ability was examined. the postantibiotic eff ...19979342422
immunopharmacological activity of polysaccharide from the pericarb of mangosteen garcinia: phagocytic intracellular killing activities.polysaccharides from the pericarbs of mangosteen, garcinia mangostana linn., was obtained by treating the dried ground pericarbs with hot water followed by ethanol precipitation (m fraction). the extract was fractionated by anion exchange chromatography on a deae-cellulose column as mde1-5 fractions. the fractions of mde3 and mde4 composed of mainly d-galacturonic acid and a small amount of neutral sugar (l-arabinose as the major one and l-rhamnose and d-galactose as the minor ones) were studied ...19979347663
immersion heat treatments for inactivation of salmonella enteritidis with intact eggs.the effects of water-bath immersion heat treatments on the inactivation of salmonella enteritidis within intact shell eggs were evaluated. six pooled strains of salm. enteritidis (ca 3 x 10(8) cfu, inoculated near the centre of the yolk) were completely inactivated within 50-57.5 min at a bath temperature of 58 degrees c and within 65-75 min at 57 degrees c (an 8.4 to 8.5-d process per egg). following the initial 24 to 35-min come-up period, semilogarithmic survivor curves obtained at 58 and 57 ...19979351225
novel selective and non-selective optical detection of microorganisms.a new instrument, capable of detecting metabolic changes due to microbiological activity, is described. optical changes in growth media are monitored in a semi-fluid zone that separates the liquid medium containing the sample. data demonstrate that common media can be utilized in conjunction with this rapid automated technology. nutrient broth with the ph dye indicator. bromocresol purple was suitable for total counts. selective media containing dyes were utilized to assess the presence or absen ...19979351264
transferable resistance to cefotaxime, ceftazidime, and aztreonam and production of extended-spectrum beta-lactamase in a strain of salmonella enteritidis. 19979352270
distribution of salmonella contamination in ten animal feedmills.detailed sampling of spillage and dust from milling equipment was carried out in nine animal feedmills, three of which were sampled twice. the salmonella isolation rate ranged from 1.1% to 41.7% of the samples and the most contaminated mills were those where the inside of the cooling systems for pellet or mash had been colonised by salmonella. a wide range of salmonella serotypes were isolated which included salmonella typhimurium and s. enteritidis. limited sampling every two weeks for an 18-mo ...19979355251
invasion of chicken reproductive tissues and forming eggs is not unique to salmonella enteritidis.experiments were conducted in which salmonella enteritidis phage type 8, phage type 2, and rdnc (reaction does not conform) or three isolates of salmonella typhimurium of diverse origin were fed to adult laying hens to determine if s. enteritidis has a selective advantage over s. typhimurium, which is now rarely isolated from chicken eggs, in its capacity to invade reproductive tissues. the results revealed that s. enteritidis and s. typhimurium may be equal in their potential to colonize the ti ...19979356697
differences in frequency, level, and duration of cecal carriage between four outbred chicken lines infected orally with salmonella enteritidis.four chicken lines, l2, b13, pa12 (egg-type), and y11 (meat-type), were tested for experimental carrier state of salmonella enteritidis (se) in two identical trials. after oral inoculation of se at 1 wk of age with 5 x 10(4) se colony-forming units (cfu), 10 chickens per line were necropsied weekly for 6 wk and then every 8 or 15 days until the 12th week postinoculation (pi). liver, spleen, ovary, and ceca were examined for level of se colonization. numbers of positive livers and spleens and lev ...19979356700
genotypic evaluation of salmonella enteritidis isolates of known phage types by arbitrarily primed polymerase chain reaction.salmonella enteritidis isolates of known phage types 8, 13a, and 14b were inoculated separately into a group of 14-wk-old specific-pathogen-free chickens. s. enteritidis isolates from pre- and postinoculum were analyzed with the use of arbitrarily primed polymerase chain reaction to determine any genotypic changes after reisolation from the chickens. the preinoculum isolates and the isolates of s. enteritidis recovered at 5, 10, 20, and 35 days postinoculation from the inoculated chickens with v ...19979356723
pcr amplification of ribosomal dna spacer regions of salmonella enteritidis isolates.polymerase chain reaction (pcr) was used to amplify ribosomal dna spacer regions from nine salmonella enteritidis field isolates. unique products of 480 and 660 bp were obtained from the isolates. pcr product (480bp) was then cloned into puc18 vector by blunt end ligation.19979357177
importance of the serovar-specific plasmid for virulence of salmonella strains in calves.to evaluate the influence of serovar-specific plasmids on salmonella virulence in calves, experiments were performed involving infection, by the oral route, with mixtures of strains containing equal counts of a plasmid-carrying and a plasmid-free strain of the same serovar. the concentration ratio between the plasmid-carrying and the plasmid-free strain which had developed in the organs of the infected animals was used for a comparative evaluation of virulence and pathogenetic behaviour of the s ...19979361383
maximizing the usefulness of food microbiology research.funding for food microbiology research often follows disease outbreaks: botulism from vacuum-packed white-fish chubs, listeriosis from soft cheeses, or illness due to salmonella enteritidis or escherichia coli. as a consequence of research, detection, identification, and subtyping methods improve, and more is learned about pathogenicity and virulence. research also explores the organisms' capacity to multiply or survive in food and to be killed by established or novel processes. however, rarely ...19979366606
salmonella lung involvement in patients with hiv infection.to determine the frequency, clinical features, and outcome of lung involvement in hiv-infected patients having nontyphoid strains of salmonella bacteremia.19979367457
fatality due to severe salmonella enteritis associated with acute renal failure and septicemia.a 66-year-old man was hospitalized for abdominal pain and diarrhea of more than 10 times a day. he had been under regular medication with prednisolone for rheumatoid arthritis. on admission, laboratory data and clinical examination indicated inflammation, dehydration, acute renal failure with a high level of serum musculogenic enzyme (creatine kinase), and ileus. salmonella enteritidis was isolated from his fecal and blood samples. the patient died within 24 hours after admission, and autopsy sh ...19979372342
dietary calcium inhibits the intestinal colonization and translocation of salmonella in rats.dietary calcium decreases the cytotoxicity of intestinal contents and intestinal epitheliolysis by precipitating cytotoxic surfactants such as bile acids. a decreased luminal cytotoxicity might not only strengthen the barrier function of the gut mucosa but also reinforce the protective, endogenous microflora. we hypothesized, therefore, that dietary calcium increases the resistance to intestinal infections.19979247475
a novel model of bacterially-induced acute anterior uveitis in rats and the lack of effect from hla-b27 expression.humans with the major histocompatibility antigen b27 (hla-b27) are especially at risk for developing rheumatic disorders such as ankylosing spondylitis and reiter's syndrome. acute anterior uveitis (aau) often occurs in association with these diseases or in hla b27 positive individuals without joint disease.19979250003
sef17 fimbriae are essential for the convoluted colonial morphology of salmonella enteritidis.salmonella enteritidis isolated from poultry infections generated a convoluted colonial morphology after 48 h growth on colonisation factor antigen (cfa) agar at 25 degrees c. a mutant s. enteritidis defective for the elaboration of the sef17 fimbrial antigen, in which the agf gene cluster was inactivated by insertion of an ampicillin resistance gene cassette, and other wild-type s. enteritidis transduced to this genotype failed to produce convoluted colonies. however, growth of sef17- mutants a ...19979252570
increase in multiple antibiotic resistance in nontyphoidal salmonellas from humans in england and wales: a comparison of data for 1994 and 1996.the incidence of multiple drug resistance (to four or more antimicrobials) in salmonellas from humans in england and wales in 1996 has been compared with corresponding data for 1994. for salmonella enteritidis multiple resistance has remained rare, although a high proportion of isolates of phage type 6a have shown resistance to ampicillin. for s. typhimurium multiple resistance has continued to increase, with 81% of isolates now multiresistant. of particular importance in s. typhimurium has been ...19979270996
salmonella enterica var typhimurium and salmonella enterica var enteritidis express type 1 fimbriae in the rat in vivo.in a series of experiments rats were dosed with purified type 1 fimbriae from salmonella enterica var enteritidis or with fimbriated cultures of either s. enterica var typhimurium or s. enterica var enteritidis. paraffin-wax embedded histological sections of jejunal and ileal tissue were taken and stained by the streptavidin biotin complex (sabc) staining technique for the detection of salmonella and type 1 fimbriae. on oral infection with enteritidis and typhimurium both bacteria were shown to ...19979271169
inhibition of in vitro growth of enteropathogens by new lactobacillus isolates of human intestinal origin.three human lactobacillus strains, coded b21060, b21070 and b21190, have recently been isolated. the strains show a series of features (acid and bile resistance, adhesion to various types of mucosal cell) which make them particularly promising for the preparation of probiotic products. in the present study, the ability of the strains to inhibit the growth of pathogens in coculture was investigated. lactobacilli were incubated simultaneously or after one overnight growth with enterotoxigenic esch ...19979271875
in vivo treatment with endotoxin increases rat pulmonary vascular contractility despite nos induction.pulmonary hypertension is a feature of clinical and experimental acute lung injury. nitric oxide (no) synthesis is increased in hyporesponsive systemic and pulmonary conductance arteries after endotoxin (lps) injection in the rat. we examined the effects of no synthase (nos) induction by lps on vascular reactivity of the isolated perfused rat lung (ipl) using the selective inducible (inos) inhibitor aminoguanidine (ag). baseline pulmonary artery pressures (ppa) were higher in the lps compared wi ...19979279254
salmonellosis. 19979282347
epidemiology of foodborne diseases: a worldwide review.acute foodborne disease infections and intoxications are much more of a concern to governments and the food industry today than a few decades ago. some of the factors that have led to this include the identification of new agents that have caused life-threatening conditions; the finding that traditional agents are being associated with foods that were of no concern previously: an increasing number of large outbreaks being reported; the impact of foodborne disease on children, the aging populatio ...19979282385
using laboratory-based surveillance data for prevention: an algorithm for detecting salmonella outbreaks.by applying cumulative sums (cusum), a quality control method commonly used in manufacturing, we constructed a process for detecting unusual clusters among reported laboratory isolates of disease-causing organisms. we developed a computer algorithm based on minimal adjustments to the cusum method, which cumulates sums of the differences between frequencies of isolates and their expected means; we used the algorithm to identify outbreaks of salmonella enteritidis isolates reported in 1993. by com ...19979284390
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