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association of infectious mononucleosis with nephrotic syndrome.a patient had infectious mononucleosis (im) associated with transient nephrotic syndrome (ns). a kidney biopsy sample studied by light and electron microscopy demonstrated minimal glomerular lesions. immunofluorescent studies revealed mainly granular mesangial deposits of igm, and to a lesser extent, deposits of igg and of c4 and c3. no epstein-barr virus-related antigen could be detected in the kidney. this, and three other cases reported in the literature, suggest a causal relationship between ...19817013729
identification of an epstein-barr virus nuclear antigen by fluoroimmunoelectrophoresis and radioimmunoelectrophoresis.a 65,000-dalton (65k) antigen found in raji cells by fluoroimmunoelectrophoresis and radioimmunoelectrophoresis has been identified as an epstein-barr virus nuclear antigen (ebna). this identification is based on the following evidence. the 65k antigen is detected in raji cells but not in three epstein-barr virus (-) human b cell lines. it is not detected with ebna (-) sera. the 65k antigen is found predominantly in the nucleus and co-elutes with ebna during partial purification by dna-sepharose ...19817017165
photoreactivation in bacteria and in skin.in many procaryotic and eucaryotic cells, photoreactivating enzyme mediates light-dependent repair of uv-induced damage: the enzyme binds to a pyrimidine dimer in dna, and, on absorption of a photon (300-600 nm), specifically monomerizes the dimer, thus repairing the dna. photoreactivating enzyme has been found in human tissues and human cells in culture; human cells in culture can photoreactivate cellular dimers, and can mediate photoreactivation of herpes (human fibroblasts) and epstein-barr v ...19817019353
adult t-cell leukemia: antigen in an atl cell line and detection of antibodies to the antigen in human sera.indirect immunofluorescence of certain human sera demonstrated an antigen(s) in the cytoplasm of 1--5% of the cells of a t-cell line, mt-1, from a patient with adult t-cell leukemia (atl), which is endemic in southwestern japan. the antigen was not detected in other human lymphoid cell lines, including six t-cell lines, seven b-cell lines, and four non-t non-b cell lines. the antigen did not show cross antigenicity with that of herpesviruses, including epstein--barr virus, herpes simplex virus, ...19817031654
monoclonal antibody against a burkitt lymphoma-associated antigen.a monoclonal antibody, referred to as 38.13, was obtained by fusing murine myeloma cells with lewis rat splenocytes sensitized with daudi cells (human burkitt lymphoma containing epstein--barr virus genome but lacking hla-a, -b, and -c and beta 2-microglobulin molecules at the cell surface). 38.13 antibody was demonstrated to be a rat igm. by complement-dependent microcytotoxicity and indirect immunofluorescence assays, 38.13 antibody was shown to react specifically with cells derived from burki ...19817031655
qualitative abnormalities in insulin binding in a patient with extreme insulin resistance: decreased sensitivity to alterations in temperature and ph.cultured lymphocytes transformed by epstein--barr virus were employed to study insulin receptors from a patient with extreme insulin resistance associated with the syndrome of leprechaunism. with cultured lymphocytes from normal subjects, insulin binding to its receptor is exquisitely sensitive to changes in temperature and ph. in cells from normal subjects, insulin binding was increased by approximately 250% as the temperature was decreased from 37 degrees c to 12 degrees c. in contrast, with c ...19817031672
selective lymphocyte deficiency in seronegative rheumatoid arthritis.prior studies have shown that in vitro infection with the epstein barr virus (ebv) is able to induce igm rheumatoid factor production by normal lymphocytes, with a higher degree of production by seropositive rheumatoid arthritis lymphocytes. the present investigation demonstrates that ebv-infected lymphocytes from patients with seronegative rheumatoid arthritis produce in vitro significantly less igm rheumatoid factor than do normal lymphocytes. the results suggest that the peripheral blood of s ...19816972764
early changes in the glycopeptides of human b-lymphocytes after epstein-barr virus infection in vitro.human b lymphocytes obtained from healthy donors were infected with epstein-barr virus in vitro. from the initiation of infection to the final establishment of a permanent lymphoblastoid cell line, fucosyl glycopeptides of the cell surface were investigated. in order of appearance the following events took place: expression of epstein-barr virus-determined nuclear antigen, mitotic activity of the cells and specific glycopeptide alterations on the cell surface. this specific alteration in glycope ...19816972922
human lymphoid cell lines and glucocorticoids: i. characterization and cytolethal responses of lymphoblastoid, leukaemia and lymphoma lines.using various genotypic and phenotypic markers 20 human lymphoid cell lines have been classified as lymphoblastoid, leukaemia or lymphoma subtypes. each cell line type exhibited characteristic morphological and behavioural properties in suspension culture. whilst most lymphoblastoid and lymphoma cell lines manifested b-cell phenotypes and contained epstein barr virus (ebv) genome, leukaemia lines demonstrated t-cell markers and lacked ebv genome. individual cell lines demonstrated unique isoenzy ...19816973456
[transformation of type t lymphoma into type b lymphoma].a 45-year-old woman with t-cell lymphoma is reported. enlargement of the peripheral lymph nodes was not observed. splenectomy was performed for hypersplenism and during the operation the mesenteric and retroperitoneal lymph nodes were found to be greatly enlarged. three years after the diagnosis of lymphoma malabsorption syndrome developed. after 5 years sudden clinical deterioration appeared, with loss of reaction to effective treatment given as yet. conversion of the predominating lymphocyte s ...19816976055
manifold expression of new cellular genes in human lymphoid neoplasia.previous studies performed in our laboratory have shown that a minor single-stranded dna component (ssdna) isolated from the bulk of double-stranded nuclear dna (ndna) of human, murine, or avian cells consisted mainly of active transcription sites. in the present work, ssdna isolated from human lymphoid cells, either malignant or not, amounted to 1.2-1.4% of the total ndna. this was labeled with 125i and utilized as a probe in rna-driven iterative hybridization and cross-hybridization assays. in ...19816947241
human erythroid cell lines derived from a patient with acute erythremia.three continuous human cell lines, designated kmoe, derived from a patient with acute erythremia (di guglielmo's disease) are reported. the cell lines are the cultures of (1) bone marrow cells, (2) peripheral blood cells, and (3) cells from a tumor developed into an athymic nude mouse after transplantation of the cultured bone marrow cells. cells of all three lines show morphology of immature erythroblast and have i(17q) marker chromosome. they are negative for both philadelphia chromosome and e ...19816949905
[serological diagnosis of virus hepatitis (author's transl)].hepatitis a is diagnosed by the detection of specific igm-antibodies. the virus itself can be found in stool specimens. gammaglobulin is useful in prophylaxis of hepatitis a. in the course of hepatitis a hbs- and hbe-antigen as well as hbc(igm and igg)-, hbs- and hbe-antibodies can be detected. by analysis of this parameters information is obtained about acute or chronic hepatitis as well as about the probability of infectivity of a blood sample. hepatitis non a non b is most important after blo ...19816795832
virological and serological study in children hospitalized for acute hepatitis (detection of hbsag, haag, anti-hav and specific igm-anti-hav).serological methods were used to detect different markers of hepatitis a and b infection in a series of 36 children aged 3.5-13 years with acute viral hepatitis, hospitalized from august 1978 to october 1978. hepatitis a was verified serologically in 30 patients (83.3%) with the demonstration of specific igm-anti-hav. hepatitis b infection was serologically confirmed in only 2 patients (5.5%). after exclusion of hepatitis a and b as well as of hepatitis due to cytomegalovirus, leptospira and eps ...19816796547
sensitization of lymphocytes against pooled allogeneic cells. ii. characterization of effector cells cytotoxic for autologous lymphoblastoid cell lines.stimulation of human lymphocytes in mixed leukocyte culture (mlc) with x-irradiated pooled allogeneic normal cells (poolx) was previously shown to result in generation of effector cells cytotoxic for autologous epstein-barr virus- (ebv) transformed lymphoblastoid cell lines (lcl). this study was undertaken to determine whether lysis of the autologous ebv- transformed lcl cells by pool-stimulated cells is mediated by cytotoxic tc lymphocytes (tc) or natural killer- (nk) like cells, both of which ...19816450239
in vitro induction of human suppressor t cells by a chorionic gonadotropin preparation.human chorionic gonadotropin (hcg) in physiological retroplacental concentration has been shown to possess the capacity of inducing human lymphocytes which are subsequently competent to depress an antibody response induced by purified protein derivative of tuberculin, phytohemagglutinin, lipopolysaccharide and pokeweed mitogen. these suppressor cells inhibited the t cell-dependent mitogen-induced activation of lymphocytes synthesizing igm, igg and iga. no suppression by hcg-induced cells was obs ...19816456339
human post-thymic precursor cells in health and disease. iii. role of the autologous rosette-forming t cells in the generation of spontaneous killer cells in the autologous mixed lymphocyte reaction.adult peripheral blood t lymphocytes activated in autologous mixed lymphocyte reactions (amlr) exerted cytotoxicity on both phytohaemagglutinin-stimulated cells and epstein-barr virus-transformed cells. when autologous rosette-forming t cells were removed from total t lymphocytes, there was no generation of cytotoxicity. re-addition of autologous rosette-forming t cells (tar cells) to a population of t cells depleted of tar cells restored the cytotoxic activity. treatment of tar cells with mitom ...19816458877
eb-virus negative infectious mononucleosis. 19816309972
a search for a characteristic illness in children with serological evidence of viral or toxoplasma infection. 19816309973
the relationship between atypical lymphocytosis and serological tests in infectious mononucleosis. 19816309974
the spectrum of epstein-barr virus disease. 19816283398
preparation of ferritin conjugates and antibodies for the localization and identification of antigens in tissues and cells by electron microscopy.ferritin, due to its high electron density, serves s a good marker in immunoelectron microscopic studies to localize antigens on and in cells and tissues. a step-by-step methodology for the conjugation of antibodies to ferritin is presented. special attention has been placed on the techniques for the purification of ferritin and antibodies, conjugation procedures, and the treatment of specimens with conjugates. methods for obtaining the separation of conjugated antibodies from unconjugated antib ...19816289422
the reliability of iga antibody to epstein-barr virus (ebv) capsid antigen as a test for the diagnosis of nasopharyngeal carcinoma (npc).since patients with nasopharyngeal carcinoma were first reported to have elevated levels of iga antibody to epstein-barr virus (ebv) in their sera, workers in a number of countries have studied the possibility that this assay could be used in the diagnosis and monitoring of patients with this disease. in the united states, a collaborative project involving seven centers has been established to investigate the potential value of iga antibody to ebv viral capsid antigen (vca) as a clinical tool. i ...19816299561
epstein-barr virus-induced malignant lymphoma in a white-lipped marmoset.one of six white-lipped marmosets inoculated with cell-free b95-8 virus developed diffuse malignant lymphoma. epstein-barr virus (ebv) dna was detected in a pathologically enlarged mandibular lymph node by dna-dna hybridization. the affected animal at the time of killing had ebv antibody titers of 1:320 for viral capsid antigen (vca) and 1:20 for early antigen (ea) while all non-diseased animals had less than or equal to 1:80 vca antibody and no detectable ea antibody. this is the first report o ...19816265385
induction of type-c retrovirus by the tumor promotor tpa.the tumor promotor 12-0-tetradecanoyl-phorbol-13-acetate (tpa) induced endogenous murine xenotropic type-c retrovirus from a1-2 cells, derived from the balb/c mouse, as determined by infectious center focus-forming assay on permissive normal rat kidney (nrk) cells. kinetic dose-response studies showed that the number of cells induced to release virus was dependent on tpa concentration and the time of assay following tpa exposure. maximal induction occurred when cells were treated with 80 ng/ml o ...19816265387
human monoclonal antibodies: their technology and application. 19816276640
titers of antibodies to rana in rheumatoid arthritis and normal sera. relationship to epstein-barr virus infection.the disease specificity of antibodies to rheumatoid arthritis nuclear antigen (rana) was examined by comparing anti-rana titers in sera from 100 patients with rheumatoid arthritis (ra) with sera from 93 healthy controls. anti-rana antibodies were found in 86% of the ra sera and 56% of the controls. the higher titers in the ra sera were unrelated to clinical features or to measurements of circulating immune complexes or rheumatoid factors. to study the relationship of these antibodies to previous ...19816275861
hexose transport in epstein-barr virus (ebv) negative lymphoma lines and their ebv converted, virus genome carrying sublines.increased hexose uptake is a marker for viral transformation, as has been shown in non-human fibroblasts transformed by oncogenic viruses. if this phenomenon is a general expression of viral induced transformation it should also apply on different oncogenic virus-cell systems. recently two human ebv-negative lymphoma lines were converted to a stable ebv-positive state by infection with ebv. according to their biochemical and biological properties they enable us to study events associated with eb ...19816275931
atypical lymphadenopathies of the head and neck.atypical lymphadenopathies fail to achieve the morphologic criteria of a malignant neoplasm, but exceed the usual concepts of follicular, lymphoid, or sinus histiocytic hyperplasias. rich cellular proliferations usually composed of prominent histiocytes, or immunoblasts, or both with or without a vascular scaffolding obscure the nodal architecture. toxoplasmosis, infectious mononucleosis, zoster, and vaccination-induced lymphadenopathies are caused by infectious agents, dermatopathic lymphadenit ...19816276076
histiocytic medullary reticulosis re-visited. 19816276269
cyclosporin a abrogates proliferation of t cells and generation of suppressor and cytotoxic t-cell function induced by epstein-barr virus.cyclosporin a (cya) promotes the outgrowth in vitro of epstein-barr-virus(ebv)-infected cells of immune donors. in the present study, the effects of cya on the t-cell responses developed to an in-vitro ebv infection were studied. cyclosporin a, by acting on the responder cells and not on stimulator cells, strongly inhibited the proliferation of t cells normally induced by ebv-infected autologous cells. moreover, t cells from cultures not exposed to cya exerted suppression on both alloantigen-ind ...19816276295
inhibitory effect of retinoids on epstein-barr virus induction in raji cells.induction of epstein-barr virus (ebv) early antigen after treatment with various combinations of croton oil and n-butyrate was markedly inhibited by retinoids 7901, 7902, ro 10-9359 and ro 11-1430. possible administration of retinoids to virus capsid antigen iga antibody-positive individuals in high-risk areas for nasopharyngeal carcinoma to prevent ebv activation and development of this cancer is discussed.19816276324
effect of protease inhibitors on the activation of epstein-barr virus repressed in cultured lymphoid cells.the effects of five microbial and one synthetic protease inhibitors on the activation of epstein-barr virus expression in latency infected lymphoblastoid cells were studied. all protease inhibitors tested suppressed both viral antigen induction by 5-iododeoxyuridine treatment and cell growth in general. among the inhibitors, however, antipain and pepstatin exerted a relatively strong and significant suppressive effect on antigen induction as compared to their inhibitory effect on cell growth.19816276326
epstein-barr virus susceptibility of b cells correlates to the presence of complement receptors but not surface immunoglobulins.nuclear antigen (ebna) inducibility by epstein-barr virus (ebv) was assessed in relation to the presence of complement (c3) receptors and surface immunoglobulins (sig) on leukemic lymphocytes. when erythrocyte-antibody-complement complex(eac)-positive, sig-negative b cells were exposed to ebv, ebna synthesis occurred with a high frequency. in contrast, no significant ebna induction was evident in sig-positive, eac-negative b cells. cells possessing both markers were ebv-infectable, in proportion ...19816276327
severe epstein-barr virus pulmonary involvement.two cases of severe epstein-barr virus (ebv) pulmonary involvement are described. both were documented by ebv-specific serology and other causes of respiratory tract infection were excluded by culture and serology. our first case describes fatal infectious mononucleosis (im) with severe pneumonia in a patient without a definable immunologic disorder. previous cases of pulmonary involvement in im are reviewed.19816276339
use of plaque assays to study thyroglobulin autoantibody synthesis by human peripheral blood lymphocytes.plaque assays have been used to study thyroglobulin autoantibody synthesis and total immunoglobulin production by cultures of peripheral blood lymphocytes from patients with hashimoto's thyroiditis. freshly isolated hashimoto or normal lymphocytes contained small numbers (7-1300) of total igg plaque-forming cells (pfc) and total igm pfc, but specific thyroglobulin antibody pfc was undetectable. after 5-8 days' culture with pokeweek mitogen (pwm), total igg and igm pfc were markedly increased to ...19816276967
characterization of alloantigen-activated human lymphocytes cytotoxic for autologous eb virus-transformed lymphoblastoid cell lines. 19816277059
infectious mononucleosis and epstein-barr virus in japan. ii. cellular aspects. 19816277135
chromosome #14 markers in two epstein-barr virus (ebv)-transformed lymphoblastoid cell lines of normal origin differ from the burkitt lymphoma (bl)-associated 14q+ marker.two among ten epstein-barr virus (ebv)-transformed lymphoblastoid cell lines contained a 14q+ marker in a low frequency of the cells (2 and 9%). by means of "mesome-prosome" analysis of the g-band patterns of these markers; it was established that the additional chromosome segments of these two 14q+ markers came from chromosomes #3 and #5, respectively, and not from chromosome #8 as in the 14q+ marker of burkitt lymphoma. chromosome #8 was not involved at all in any changes in the ten lymphoblas ...19816277469
studies on long-term t-cell-mediated immunity to epstein-barr virus in immunosuppressed renal allograft recipients.peripheral blood lymphocytes from normal seropositive donors and renal transplant recipients on various immunosuppressive regimens have been tested for their ability to mount a cytotoxic response when cultured with autologous eb virus-infected b cells and thereby to cause regression of proliferating b-cell foci. cultures from 10 normal seropositive donors all showed the normal pattern of regression. lymphocytes from patients receiving cyclosporin a therapy with or without prednisolone completely ...19816277797
cell surface glycoprotein patterns of two ebv-negative lines and their ebv converted sublines.cell surface galactosyl-glycoprotein patterns were compared between ramos and bjab, two epstein-barr virus (ebv)-negative burkitt lymphoma lines, and their acutely ebv-superinfected and stably ebv-converted derivatives. a major difference between ramos and its ebv-infected variants was the appearance of a 69,000 mol. wt. galactosyl-glycoprotein (gp69) in the latter. the same phenomenon was found in bjab along with the additional appearance of a 71,000 mol. wt. galactosyl-glycoprotein (gp71) in t ...19816277822
epstein-barr virus receptor expression is correlated to cell cycle phase.epstein-barr virus (ebv) receptor positive cells absorbed fitc-conjugated virions and were subsequently stained for dna content with propidium iodide. these cells were analyzed by flow cytometry to determine the relationship between cell cycle and ebv receptor (ebvr) expression on single cells. ebvr was present throughout the entire cell cycle. the level of expression, however, varied; increasing in g1 and g2/m while remaining approximately constant in s phase. analysis of receptor density in te ...19816278141
[the cytotoxic effect of lymphocytes from patients with nasopharyngeal cancer (npc) against a npc epitheloid cell line (author's transl)]. 19816273085
hodgkin's disease following infectious mononucleosis. a case report. 19816273176
maturation of b-cell differentiation ability and t-cell regulatory function in infancy and childhood. 19816273292
ebv-positive burkitt's lymphoma from algeria, with a three-way rearrangement involving chromosomes 2, 8 and 9.burkitt's lymphoma was diagnosed in male algerian child presenting with abdominal and jaw tumours. viral studies revealed that this non-endemic case was associated with the epstein-barr virus. cytogenetic examination of tumour cells showed a complex three-way rearrangement involving chromosomes 2, 8 and 9. our report indicates that, in burkitt's lymphoma, not only simple but also complex translocation involving chromosome 8 may arise. this finding in a north african case emphasizes the need for ...19816273331
diploid human lymphoblastoid and burkitt lymphoma cell lines: susceptibility to murine nk cells and heterotransplantation to nude mice.human lymphoid cell lines which had been classified on the basis of studies on clonality and morphological, on the basis of studies on clonality and morphological, chromosomal and functional parameters as lymphoblastoid cell line (lcl) of presumed non-neoplastic origin and burkitt lymphoma (bl) lines of proven malignant origin, were tested for susceptibility to natural killer (nk) cells obtained from the spleens of athymic nude mice. the 20 lines included normal diploid lcl and aneuploid bl line ...19816273332
kaposi's sarcoma. iv. detection of cmv dna, cmv rna and cmna in tumor biopsies.in order to determine whether human cytomegalovirus- (cmv) dna homologous sequences as well as cmv-specific rna(s) and antigen(s) exist in tumor biopsies of kaposi's sarcoma (ks) dna-dna reassociation, rna-dna in situ cytohybridization and anticomplement immunofluorescence test (acif) tests were applied. three of 10 dnas extracted from kaposi sarcoma biopsies contained dna sequences homologous to radioactively labelled human cmv dna probe. the amount of cmv dna in these sarcoma tissues was calcu ...19816273333
steroids inhibit tumor promoting agent induced epstein-barr virus early antigens in raji cells.four steroids and one protease inhibitor were evaluated for their effects on 12-o-tetradecanoyl phorbol 13-acetate (tpa)-induced and epstein-barr virus-induced early antigens (ebv-ea) in raji cells. continuous treatment with dexamethasone, prednisolone, hydrocortisone and cortisone inhibited tpa-induced ebv-ea to varying degrees, but the protease inhibitor n-alpha-p-tosyl l-lysine chloromethyl ketone-hcl (tlck) had no significant effect. none of the agents tested inhibited ebv- induced ea. in ad ...19816273335
use of cryopreserved lymphocytes for the indirect leukocyte migration inhibition assay.production of leukocyte migration inhibitory factor (lif) by fresh and cryopreserved lymphocytes from the same donors was detected by indirect leukocyte migration inhibition (lmi) assay. the same results were obtained when fresh and frozen lymphocytes were tested in parallel. this indicates that cryopreservation does not impair the ability of lymphocytes to produce lif.19816273472
epidemiologic, etiologic and immunologic aspects of nasopharyngeal carcinoma [npc]. 19816273487
clinical, virologic, and serologic evidence of epstein-barr virus infection in association with childhood pneumonia.to explore the association of epstein-barr virus infection with childhood pneumonia we studied two patients whose mononucleosis-like illnesses were accompanied by pneumonia; both had virologic and serologic evidence of current or recent ebv infection. we then analyzed the sera of 71 children (age range, 14 months to 9 years) with pulmonary infiltrates for the presence of four classes of antibody to ebv. antibody responses consistent with current or recent eb virus infection were found in 15. two ...19816273517
epstein-barr virus-induced lymphoblastoid cell lines from seropositive adult donors and their hla-antigens.epstein-barr virus (ebv)-induced lymphoblastoid cell lines (lcl) were established from seropositive adult japanese donors. hla-a, -b, -c and -dr antigens expressed on these ebv-lcl cells were identical to those of peripheral blood lymphocytes. there were no extra reactions upon dr typing; the extra reactivity in a, b and c typing was due to contaminating dr antibodies in the typing sera. in one-way mixed lymphocyte reactions with a stimulator : responder ratio between 1 : 10 and 1 : 20, autologo ...19816273617
t cell growth factor from human splenic cell cultures: conditions for its production, and its utilization for maintenance of cytotoxic t cell lines.we prepared the t cell growth factor (tcgf) from human spleen cell cultures stimulated with phytohemagglutinin (pha). various cell culture conditions and agents supporting the active tcgf production of the spleen cells were examined. the highest tcgf activity was obtained in the supernatants under the conditions that 2 x 10(6)/ml spleen cells were stimulated with pha for 48 hr. production of tcgf from spleen cells depended markedly on their individual sources. addition of indomethacin to the cul ...19816273698
[primary epstein-barr virus infection in an aged patient (author's transl)]. 19816274973
epstein-barr virus dna. x. direct repeat within the internal direct repeat of epstein-barr virus dna.the 3,360-base-pair internal direct repeat (ir) in epstein-barr virus dna separates the short and long unique dna domains. ir has a single bamhi site. the juncture between the short unique domain and ir has been mapped by restriction endonucleases and is less than 2,600 nucleotides before the bamhi site in ir. the junction between ir and the long unique domain has been sequenced and is approximately 650 nucleotides after the bamhi site in ir. thus, relative to the start of ir at the juncture wit ...19816275099
expression of epstein-barr viral early antigen in monolayer tissue cultures after transfection with viral dna and dna fragments.antigens associated with the epstein-barr virus (ebv) replicative cycle were found in the nucleus and cytoplasm of human placental, vero, bsc-1, and owl monkey kidney cells transfected with ebv dna prepared from several different strains of virus. the number of antigen-positive nuclei increased when transfection was followed by cell fusion induced by inactivated sendai virus. about 1,200 antigen-positive foci were induced per micrograms of ebv dna. on the basis of their reactivity with various w ...19816275121
cloning overlapping dna fragments from the b95-8 strain of epstein-barr virus reveals a site of homology to the internal repetition.overlapping, sheared dna fragments from the b95-8 strain of epstein-barr virus were cloned in charon 4a. eleven recombinant phages plus one recombinant plasmid contained all of the sequences found in b95-8 virion dna. analysis of recombinant dna molecules revealed a previously undetected site of homology to the internal repetition found in epstein-barr virus dna. this site was adjacent to or at a site which was unstable when the recombinant dna was propagated as phage dna in procaryotic hosts.19816275132
[eb virus receptor test]. 19816275145
[immunologic changes in patients with hodgkin's disease in clinical remission and recurrence]. 19816275237
complement-fixing antibodies to human cytomegalovirus induced early nuclear antigens in mononucleosis.early nuclear antigen (cmna) induced by human cytomegalovirus (hcmv) was extracted from infected human embryonic fibroblast cells and purified by ds dna sephadex chromatography. the purified antigen was added to acid-fixed preparations from human embryonic fibroblasts and these in vitro converted nuclei were exposed to human sera to estimate the antibodies to cmna by anti-complement immunofluorescence staining. serial serum samples were obtained from eight patients suffering from acute hcmv infe ...19816275252
epstein-barr in acute nontraumatic mononeuropathies. 19816275310
the epstein-barr virus: historical, biologic, pathologic and oncologic considerations. 19816275338
ebv infections in brazil. iii -- infections mononucleosis. 19816275484
[seroepidemiology of epstein-barr virus]. 19816275491
[diagnosis of peripheral adenopathy in children]. 19816275518
epstein-barr (eb) virus antibodies in rheumatoid arthritis. 19816275562
a sequential study of human b lymphocyte function from birth to two years of age.the immunoglobulin synthesizing capacity of individual lymphocytes from newborns and infants aged 2, 6, 12 and 24 months respectively has been studied. the technique has been to expose purified lymphocytes to a t lymphocyte dependent (pokeweed mitogen) or a t lymphocyte independent (epstein-barr virus) activator of b lymphocytes. activation has been measured as immunoglobulin secretion of individual b lymphocytes using a hemolytic plaque assay. b lymphocytes from newborns can be made to synthesi ...19816275657
identification of antigenic determinants unique to the surfaces of cells transformed by epstein-barr virus. 19816273741
[leukocyte migration inhibition evoked by epstein barr virus antigen. i. immune response in healthy donors]. 19816273776
an epstein--barr virus early protein induces cell fusion.superinfection of raji cells with epstein--barr virus (ebv) leads to syncytium formation. studies using metabolic inhibitors and amino acid analogues suggest that the fusion-inducing factor belongs to the early group of virus-specified proteins. induction of early ebv protein synthesis in raji cells by using various chemicals also leads to syncytium formation, indicating that the fusion process is not caused by a virion membrane protein introduced into the cells upon infection. the relevance of ...19816273915
quantitative analyses of specific b cells for sheep red blood cell, phosphorylcholine, and hepatitis b surface antigen in human b cell populations by polyclonal transformation with epstein-barr virus.an experimental system was developed to analyse quantitatively specific b cells for various antigens in human b cell populations by polyclonal transformation and immunoglobulin production induced by in vitro infection with epstein-barr virus (ebv). ebv-infected cells were cultured in microtest plates for three weeks and specific antibody activities for sheep red blood cell (srbc) were detected in the culture supernatants by hemolysis and those for phosphorylcholine (pc) and for hepatitis b surfa ...19816274056
lymphoblastoid cell lines, transformed by epstein-barr virus, in the enzymatic study of hereditary lysosomal storage diseases.assay conditions were studied for eight lysosomal enzymes in lymphoblastoid cell lines transformed by epstein-barr virus. the transformed lymphoblastoid cells retained all eight enzyme activities, though the levels sometimes differed from those in the peripheral lymphocytes or granulocytes. the levels of these eight lysosomal enzymes were measured in lymphoblastoid cells from 11 patients with hereditary lysosomal storage diseases--gmi-gangliosidosis, a variant of beta-galactosidase deficiency (s ...19816274059
burkitt's lymphoma in japan: clinicopathological features of twenty-two patients.the clinicopathological features of 22 japanese patients with burkitt's lymphoma were reviewed. there were 14 males and 8 females with a mean age of 9.5 years. jaw tumors dominated the clinical picture at the time of diagnosis with abdominal tumors second in frequency. the central nervous system and bone marrow were involved during the course of the disease in 32% of the patients. the anti-epstein-barr (eb) virus antibody titers in japanese patients were comparable to those found in control grou ...19816274060
host cell reactivation of uv- and x-ray-damaged herpes simplex virus by epstein-barr virus (ebv)-transformed lymphoblastoid cell lines. 19816274084
induction of epstein-barr virus early antigens by intercalating chemicals in b95-8 cells. 19816274090
epstein-barr virus-induced deoxynuclease and the reutilization of host-cell dna degradation products in viral dna replication. 19816274091
epstein-barr virus-induced early polypeptides in raji and nc37 cells activated by diterpene ester tpa in combination with n-butyrate. 19816274093
correlation between mixed lymphocyte cultures and anti-epstein barr virus (ebv) antibodies in hodgkin's disease and non hodgkin lymphoma patients.cellular and humoral immunity was examined in hodgkin's disease and non hodgkin lymphoma (33) patients and compared with a normal group. mixed lymphocyte cultures (mlc) were used as parameter for cell mediated immunity and anti-epstein barr virus (ebv) antibodies for specific humoral immunity. high stimulation indices coincided with low anti-vca and anti-ebna titers in the control group (r = -0.343). this negative correlation was not found in non hodgkin lymphomas and was substantially lower (r ...19816274439
malignant lymphoproliferative diseases induced by epstein-barr virus in immunodeficient patients, including x-linked, cytogenetic, and familial syndromes. 19816274510
anticomplement immunoenzymatic method of detecting epstein-barr nuclear antigen in nasopharyngeal carcinoma cells and normal epithelial cells. 19816274591
studies on the toxicity and binding kinetics of abrin in normal and epstein barr virus-transformed lymphocyte culture-i: experimental results - 1.the effects of treatment with varying doses of abrin, a d-galactose binding lectin, on dna and protein synthesis of normal and epstein barr virus-transformed lymphocytes have been investigated. activation, stimulation and relative toxicity factor indices are studied as well as possible relationships between the dna and protein synthesis rates, as measured by simultaneous 3h-tdr and 14c-leucine uptake.19816274716
studies on toxicity and binding kinetics of abrin in normal and epstein barr virus-transformed lymphocyte culture-i: experimental results - 2.the effects of treatment with varying doses of abrin, a d-galactose binding lectin, on dna and protein synthesis of normal and epstein barr virus-transformed lymphocytes have been investigated. studies of activation and stimulation indices, as well as two new indices; the metabolic self and cross-coupling indices, lead to the prediction that there are three morphologically distinct subpopulations of ebv-transformed lymphocytes with different abrin binding site numbers. this conclusion is support ...19816274717
studies on the toxicity and binding kinetics of abrin in normal and epstein barr virus-transformed lymphocyte culture-i: experimental results - 3.the effects of treatment with varying doses of abrin, a d-galactose binding lectin, on dna and protein synthesis of normal and epstein barr virus-transformed lymphocytes have been investigated. using data on ebv-transformed lymphocyte cell density as a function of time and dose of abrin, one can demonstrate that the mean number of sites bound/ebv-lymphocyte needed to exert a biological influence upon the cell dna synthesis lies between 59,264 and 370,000 sites/cell. using a simple packing model, ...19816274718
some recent trends in studies of human lymphoid cells: b-cells, epstein-barr virus, and transformation. 19816274744
epstein-barr virus: its site of persistence and its role in the development of carcinomas. 19816274745
immunopathology of the x-linked lymphoproliferative syndrome.the immunopathogenesis of 25 kindreds affecting 100 males with the x-linked lymphoproliferative syndrome (xlp) is being studied comprehensively by our registry and laboratory group. xlp is a combined variable immune deficiency with epstein-barr virus (ebv) induced phenotypes of: (1) fatal infectious mononucleosis (im), (2) chronic im progressive to malignant lymphoma, (3) acute im progressive to acquired agammaglobulinemia or (4) malignant lymphoma. cytogenetic studies of peripheral blood lympho ...19816274747
the relative role of viral transformation and specific cytogenetic changes in the development of murine and human lymphomas. 19816274749
induction of epstein-barr virus by an new tumor promoter, teleocidin, compared to induction by tpa.the effect of teleocidin, a new, naturally occurring tumor promoter on induction of epstein-barr virus (ebv), was compared with that of a known tumor promoter, 12-o-tetradecanoyl-phorbol-13-acetate (tpa). early antigen (ea) and/or capsid antigen (vca) of ebv was induced in the ebv genome-carrying cell lines c-6 and p3hr-i cells by teleocidin, its effect being maximal at a concentration of 12.5 ng/ml. the production of infectious ebv from p3-hr-i cells was enhanced by teleocidin maximally at a co ...19816274809
establishment of human cytotoxic t-cell lines specific for epstein-barr virus-transformed autologous cells.ebv-specific cytotoxic t cells (tc) induced in vitro have continuously proliferated in vitro for over 9 months. the long-term maintenance of the tc growth was dependent on periodic supplementation of both irrediated ebv-transformed autologous lymphoblastoid cell line (lcl) cels and conditioned medium. the latter was derived from supernatants of human spleen-cell cultures stimulated with phytohemagglutinin. the cultured tc maintained significant cytotoxic activity to autologous lcl cells but not ...19816274810
association of epstein-barr virus with nasopharyngeal carcinoma in alaskan native patients: serum antibodies and tissue ebna and dna.biopsy specimens from alaskan native patients with nasopharyngeal carcinoma (npc) and from other patients seen on the otolaryngology service were tested for epstein-barr virus-specific dna and nuclear antigen (ebna). serum samples from both groups were tested for various ebv-related antibodies. ebv dna and ebna results were in agreement in 29 of 31 tissue specimens tested by the two methods. ten of 11 biopsies containing npc cells were positive for ebv dna. two npc patients had biopsies that sho ...19816274814
the relationship between tumor promoter binding and epstein-barr virus induction in human lymphoblastoid cell lines.the tumor promoter tpa (12-0-tetradecanoyl-phorbol-13-acetate) efficiently induces the synthesis of epstein-barr virus (ebv) antigens in ebv-genome-harboring human lymphoblastoid cells. we attempted to obtain information on the binding of tpa to cells and on the relationship between tpa-binding and ebv induction by the use of tritiated tpa (3h-tpa). our data show: (1) in the absence of cells tpa can bind to serum completely within 60 minutes. (2) cells can compete for a proportion of serum-bound ...19816274878
incorporation into dna of the base analog 2-aminopurine by the epstein-barr virus-induced dna polymerase in vivo and in vitro.the epstein-barr virus (ebv)-induced intracellular dna polymerase was assayed in vitro for the ability to utilize the mutagenic nucleotide analog 2-aminopurine deoxyribose triphosphate (d2aptp), incorporating it as the corresponding monophosphate into dna or poly[d)(a-t)] template. bacteriophage t4, lymphocyte alpha, and the ebv particle-associated dna polymerases were assayed simultaneously for direct comparison. unlike these three polymerases, which were capable of distinguishing between d2apt ...19816278464
[hepatitis caused by cytomegalovirus].epidemical, clinical, biochemical, cytological and immunological aspects are studied in ten cases of hepatitis occurred endemically in a chirurgical ward between cancerous operated patients. three were hbsag positive. none of the others seven had clinical or serological dates enough to attribute them to a nor b virus hepatitis. the clinical and serological data exclude also epstein-barr virus or toxoplasma aethiology. nevertheless, the objective results of the study have entity enough to classif ...19816278554
cloning of functional human t lymphocytes by limiting dilution: impact of filler cells and interleukin 2 sources on cloning efficiencies.conditions for the cloning of alloactivated human lymphocytes by limiting dilution in the presence of interleukin 2 (il 2) and filler cells have been investigated. cloning efficiencies were extremely high (at least 40%) when il 2 derived from pooled peripheral blood mononuclear cells (pbm), stimulated with phytohaemagglutinin in the presence of irradiated epstein-barr virus-transformed lymphoid cell lines (lcl), was used in combination with irradiated pooled pbm as filler cells. cloning efficien ...19816278580
estimation of b-cells transformed by epstein-barr virus in patients with congenital agammaglobulinemia.in vitro immunoglobulin synthesis was measured in lymphocytes from four patients with congenital agammaglobulinemia (ca gamma) stimulated by two different polyclonal b-cell activators, pokeweed mitogen (pwm) and epstein-barr virus (ebv). in pwm-stimulated cultures, patient t-cells treated with mitomycin c (mmc) were able to help the immunoglobulin (ig) synthesis of normal b-cells. patient b-cell-enriched fraction not containing surface ig positive cells did not produce ig in combination with mmc ...19816278674
dna-repair synthesis in ataxia telangiectasis lymphoblastoid cells.the ability of a number of epstein-barr virus-transformed lymphoblastoid cells from ataxia telangiectasis (at) patients to repair gamma-radiation damage to dna was determined. all of these at cells were previously shown to be hypersensitive to gamma-radiation. two methods were used to determine dna-repair synthesis: isopycnic gradient analysis and a method employing hydroxyurea to inhibit semiconservative dna synthesis. control, at heterozygote and at homozygote cells were demonstrated to have s ...19816278301
an ebv-associated, swedish case of burkitt-type malignant lymphoma.morphological, serological, dna-homology and immunofluorescence studies of a rapidly fatal malignant lymphoma in a 25-year-old sweden male revealed most of the characteristics of an ebv-associated burkitt lymphoma. the various findings are discussed in relation to previously reported observations on endemic (african) and non-endemic burkitt lymphomas.19816278823
prospective study of epstein barr virus (ebv) infection during pregnancy.the serological ebv profile of 2752 pregnant women characterized in most cases a latent ebv carrier state. the pregnancy rarely reactived this latent infection. but mothers with an "active" ebv serology gave birth more frequently than others to still born or defective bodies. in six pregnancies with primary ebv infection, detected early, four presented a pathological delivery. furthermore the follow up of the ebv profile in 719 mothers revealed a significant relation between defective births and ...19816279193
the isolation of the antibody moieties of immune complexes from serum by the pepsin digestion of conglutinin-anti-conglutinin complexes.a technique is described which allows the antibodies of circulating immune complexes to be isolated as their f(ab')2 fragments. the method is based on the precipitation of the complexes by the sequential addition of conglutinin and anti-conglutinin, and the subsequent digestion of these precipitates by pepsin. using this technique it has been possible to show antibodies to epstein-barr (eb) virus antigens in the immune complexes of patients with burkitt's lymphoma and to microbial antigens in tw ...19816279342
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