Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| infectious muscle disease. | infectious muscle diseases have very different aetiologies. the viral myositides are proved by clinical and laboratory evidences in various etiologic settings (influenza a and b, coxsackie and hiv). the bacterial myositis was considered in the near past a tropical disease, but in our days with migration of people from south to north and the endemia of aids it became a problem of the "civilized" world. on the other hand, tuberculous endemia in central-eastern europe, including romania, results in ... | 2006 | 17236294 |
| [biology, species biodiversity and distribution of trichinella nematodes]. | from the time of the discovery of trichinella larvae in 1835 until the middle of the next century it was commonly assumed that all trichinellosis was caused by a single species trichinella spiralis. this species is an intracellular parasite in both a larva and an adult stage. the l1 larvae live in a modified skeletal muscles. the adult worms occupy a membrane-bound portion of columnar epitelium, living as intramulticellular parasite. more than century later t. spiralis have been reported from mo ... | 2006 | 17432238 |
| [methods and tools for parasite differentiation within the genus trichinella]. | this review summarizes the major biological, biochemical and molecular methods which have been developed during last 20 years to distinguish parasites of the genus trichinella. from the time of the discovery of trichinella in 1835 until the 1970, it was assumed that trichinellosis was caused by a single species of parasite, trichinella spiralis. many biological parameters have been compared to differentiate the parasite, such as host specificity, geographical distribution, reproductive abilities ... | 2006 | 17432239 |
| [the influence of the procedure of excretory-secretory l1 trichinella spiralis antigen preparation on the efficiency of an elisa test in pigs]. | trichinellosis is a parasitic zoonosis transmitted to humans by consumption of raw or undercooked meat from animals infected by worms of the trichinella genus. every year seropositive cases are found among the human population and thus trichinellosis still remains an epidemiologically important disease in poland. the usefulness of elisa for anti-t. spiralis igg detection in pigs is still limited by the nature of antigen. the objective in the present study was to compare the usefulness of excreto ... | 2006 | 17432246 |
| the estimation of different elisa procedures for serodiagnosis of human trichinellosis. | the most important confirmative diagnostic test for trichinellosis is the presence of the muscle larvae in a tissue biopsy but this direct method has a low sensitivity of light and moderate infections. the aim of presented study was to compare the usefulness of the results obtained by three elisa procedures for trichinella spp. diagnosis in human outbreaks. | 2006 | 17432247 |
| [influence of free radicals on trichinella spiralis infection in mice]. | the aim of the study was to examine the influence of free radicals: nitric oxide (no), hydrogen peroxide (h202) and superoxide anion (o2-) on trichinella spiralis infection in mice. the studies were performed on two strains of mice: c57bl/6 and balb/c, which differ in immunological response to t. spiralis infection. also the influence of ag--inhibitor of inducible nitric oxide synthase (inos) administered in the first days after t. spiralis infection (1-5 dpi) on the cytotoxic immune response an ... | 2006 | 17432248 |
| trichinellosis in human and animals--new challenge. proceedings of a conference, warsaw, poland,2006. | 2006 | 17511047 | |
| [analyzing the time course of changes in the titers of specific antibodies while immunization of animals with the biological agent egfb with different adjuvants]. | the recombinant echinococcus antigens egf and egb were prepared and purified by gel filtration and ion-exchange chromatography (800 and 780 mg, respectively being obtained). four vaccinating complexes, the major component of which were the recombinant antigens egf and egb, were developed and tested in an experiment on rabbits. the time course of changes in antibody genesis was studied during a single immunization of the animals with the biological agent egfb with different adjuvants for 120 days ... | 2006 | 17290902 |
| [in vivo development of trichinella spiralis in rats]. | the causative agent is trichinella spiralis which lives in the intestines in adult form or in skeletal muscles in larval form. after ingestion of raw or undercooked infected meat that contains larvae of trichinella, these larvae enter the blood and the lymphatic circulation. they usually settle in the skeletal muscles and develop further. this study was conducted in the department of parasitology of the dokuz eylul university, school of medicine. the aim of this experimental study was to develop ... | 2006 | 17309030 |
| [study on genetic approach in the detection of cryptosporidium parvum and giardia lamblia in acquired immunodeficiency syndrome patients]. | to establish genetic method in detecting cryptosporidium parvum and giardia lamblia which often coinfected with aids patients. | 2006 | 17343184 |
| [serum il-12 level in mice infected with trichinella spiralis]. | kunming mice were infected by feeding 150+/-5 larvae of trichinella spiralis, established was also a normal control group. blood was collected from the ophthalmic venous plexus respectively on 7 d, 21 d, 35 d and 49 d after infection and il-12 in the serum was detected by elisa. the level of il-12 in serum decreased in groups of 7 d, 21 d, and 35 d, with a significant difference to the control (p<0.01). however, there was no significant difference between 49d group and control (p>0.05), suggesti ... | 2006 | 17366986 |
| biology and genome of trichinella spiralis. | clade i nematode species in the genus trichinella can cause infections in humans that lead to mortality and serious morbidity. there are currently eight recognized species or genotypes that comprise this genus. the species display diverse biological characteristics, the evolutionary significance of which recently has been extensively clarified. some of that diversity translates into variable importance as zoonotic pathogens, with t. spiralis having the highest significance. trichinellosis has re ... | 2006 | 18050431 |
| evaluation of a fluid versus a powder pepsin formulation to detect trichinella spiralis larvae in meat samples by a digestion technique. | pepsin powder constitutes a health risk, potentially causing severe allergic reactions to those handling the chemical. a fluid pepsin formulation was produced and tested, first in a preliminary study and then in a ring trial encompassing four european national reference laboratories (nrls). the purpose of each trial was to ascertain and compare the action of pepsin powder with that of the pepsin fluid for digesting meat and liberating encapsulated trichinella spiralis larvae for subsequent count ... | 2007 | 18095451 |
| contrast staining of trichinella spiralis larvae in fresh, frozen and formalin preserved muscles. | fresh, frozen-thawed and formalin-preserved muscle samples heavily infected with trichinella larvae were cut into several pieces and stained with giemsa and leishman and the reference haematoxylineosin (h & e) stain. observation under microscope revealed that both muscle larvae and nurse cells in fresh and formalin preserved specimens appeared as purplish blue structures contrasting with the pinkish color of non-infected muscle fibers in both giemsa and leishman stains. these findings were confi ... | 2007 | 18383802 |
| study of the reproductive capacity of trichinella spiralis recovered from experimentally infected mice under-dosed with albendazole or mebendazole. | the reproductive capacity of trichinella spiralis recovered from experimentally infected mice under-dosed with albendazole (alb) or mebendazole (meb) was studied. different groups of male c57/bl mice were infected with 10 +/- 0.5 muscular larvae (ml) per gram of body weight and treated with a single dose by oral (20 mg/kg) of alb, meb or praziquantel (pzq) given at 5th day post infection (dpi), during the intestinal phase of infection. in other group of mice, treatment with the same drugs and do ... | 2007 | 18209715 |
| [prokaryotic expression of trichinella spiralis gene ts21 and identification of the recombinant protein]. | to express the antigen gene ts21 of trichinella spiralis, purify the recombinant protein and test its antigenicity. | 2007 | 18441887 |
| [study on 5s rdna sequence of two isolates of trichinella from guangxi]. | to analyze 5s ribosomal dna (5s rdna) sequences of two trichinella isolates from guangxi. | 2007 | 18038783 |
| [determination of trace elements in hair of mice infected with trichinella spiralis]. | mice were divided into 3 groups: heavy infection group with 80 mice each was fed with 400 muscle larvae of trichinella spiralis, light infection group with 60 mice each was fed by 200 larvae, and uninfected control (60 mice) . the content of cu, zn and fe in the dorsal hair samples was measured in the week of 1, 3, 5, 7, 9, 11, 13 and 15 after infection. results indicated that the content of zn, cu and fe in the two experimental groups reduced considerably in comparison to the control (p < 0.05) ... | 2007 | 18038813 |
| contributions to the study of trichinella spiralis tsl-1 antigens in host immunity. | the observation on different hosts infected with trichinella spiralis that recognized similar muscle larvae (ml) antigens and the fact that different monoclonal antibodies (mab) had a similar reactivity to ml components prompted a proposal to define a useful classification system for these antigens. for this purpose, an international workshop provided a platform for the classification of t. spiralis antigens. ml antigens were classified in eight groups -- trichinella spiralis larvae groups, tsl- ... | 2007 | 18042172 |
| enhancement of bioavailability and anthelmintic efficacy of albendazole by solid dispersion and cyclodextrin complexation techniques. | the objective of this study was to improve the oral bioavailability and therapeutic efficacy of albendazole (abz) employing solid dispersion and cyclodextrin complexation techniques. solid dispersion (dispersion) was prepared using abz and polyvinylpyrrolidone (pvp) polymer (1:1 weight ratio). ternary inclusion complex (ternary complex) was prepared using abz, hydroxypropyl beta-cyclodextrin (hpbetacd) and l-tartaric acid (1:1:1 molar ratio). in rabbits with high gastric acidity (gastric ph appr ... | 2007 | 17867556 |
| time course of neural and contractile disturbances in a rat model of colitis induced by trichinella spiralis. | colitis induced by trichinella spiralis in rat induces alterations in the spontaneous motor pattern displayed by circular colonic muscle [auli, m., fernandez, e., 2005. characterization of functional and morphological changes in a rat model of colitis induced by t. spiralis. digestive diseases and sciences 50(8), 1432-1443]. we examined the temporal relationship between the severity of inflammation and the altered contractility of the underlying circular muscle as well as the role of nanc inhibi ... | 2007 | 17870133 |
| [effect in vitro of albendazole on the kinetics of cytosolic glutathione transferase from the rat liver]. | since the idea of multifunctional mode of action of anthelmintics is considered and in experimental trichinellosis in vivo albendazole seems to act as an allosteric activator of cytosolic gst from mice muscles, in this study a termosensitivity after in vitro incubation with albendazole of purified commercial cytosolic glutathione transferase (gst) from the rat liver was investigated. | 2007 | 17912804 |
| [the usefulness of elisa test for early serological detection of trichinella spp. infection in pigs]. | trichinellosis is a parasitic zoonosis transmitted to humans through consumption of raw or undercooked meat from animals infected with nematodes of the trichinella genus. every year seropositive cases are found among the human population and thus trichinellosis still remains an epidemiologically important disease. the first step of the study was the optimization of a new elisa method enabling an early and specific serological diagnosis of trichinellosis in pigs and wild boars using excretory-sec ... | 2007 | 17912813 |
| [antibodies in trichinosis and duration of their detection in convalescents]. | 2007 | 17912840 | |
| [detection of coproantigens in experimental trichinosis]. | 2007 | 17912841 | |
| the cys-loop ligand-gated ion channel gene family of brugia malayi and trichinella spiralis: a comparison with caenorhabditis elegans. | nematode cys-loop ligand gated ion channels (clgic) mediate neurotransmission and are important targets for anthelmintics in parasitic nematodes. the clgic superfamily in nematodes includes ion channels gated by acetylcholine, gamma-amino butyric acid (gaba), glutamate, glycine and 5-ht. the macrocyclic lactones and the nicotinic agonists are important groups of anthelmintics that target the glutamate gated chloride channels and the nicotinic acetylcholine receptors, respectively. the model orga ... | 2007 | 17952476 |
| [comparative immunochemical analysis of a complete somatic extract, somatic fractionated and excretory-secretory proteins and antigens of t. spiralis larvae]. | the paper deals with the analysis of the protein composition of invasion trichinella larvae, the immunochemical characteristics oftrichinella antigens of varying molecular mass, the development of the optimum procedures for having the preparative quantities of immunogenic, the so-called major trichinella antigens that are the major component for the production of diagnostic and prophylactic agents. twenty nine protein bands were detected in the composite of a complete somatic trichinella extract ... | 2007 | 17657951 |
| [evaluation of the trichellocidal activity of lantirin (imp-17)]. | 2007 | 17663043 | |
| [detection of soluble antigens in muscle tissue in experimental trichinosis]. | 2007 | 17663044 | |
| contrasting effects of trichinella spiralis and trichuris muris antigens on the infection by leishmania infantum in balb/c mice. | the interaction of trichinella spiralis and trichuris muris derived antigens with the infection by leishmania infantum was investigated in balb/c mice. infection with 10(6) promastigotes of l. infantum did not induce relevant serum antibody (igg subclasses), nor cytokine (ifn-gamma, il-4) responses despite that mice could partially control the infection. immunization with t. spiralis activated a moderate igg1 and secondarily an igg2a anti-leishmanial response whereas immunization with t. muris e ... | 2007 | 17679099 |
| use of proficiency samples to assess diagnostic laboratories in france performing a trichinella digestion assay. | routine diagnosis of animal trichinellosis for food safety and trade relies on a method of artificial digestion to free trichinella muscle larvae from meat for subsequent identification by microscopy. as part of a quality control system, the french national reference laboratory (nrl) initiated ring trials to determine the sensitivity of the test performed in the 72 routine diagnostic laboratories in france. a method was devised to obtain calibrated meat samples containing known numbers of capsul ... | 2007 | 17685343 |
| aberrant mucosal mast cell protease expression in the enteric epithelium of nematode-infected mice lacking the integrin alphavbeta6, a transforming growth factor-beta1 activator. | infection of mice with the nematode trichinella spiralis triggers recruitment and differentiation of intraepithelial intestinal mucosal mast cells expressing mouse mast cell protease 1 (mcpt-1), which contributes to expulsion of the parasite. expression of mcpt-1 is transforming growth factor (tgf)-beta1-dependent in vitro. tgf-beta1, which is secreted within tissues as a biologically inactive complex with latency-associated peptide, requires extracellular modification to become functionally act ... | 2007 | 17702893 |
| application of giemsa stain for easy detection of trichinella spiralis muscle larvae. | the application of giemsa technique to stain compressed diaphragm samples obtained from rodents experimentally infected with trichinella spiralis is described. diaphragm samples from rats heavily infected with 20 muscle larvae per gram of body weight (20 ml/gbw) were cut into several pieces and stained with giemsa; on the other hand, whole diaphragms from slightly infected mice (1 ml/gbw) were also stained with giemsa. besides, muscle samples were also stained with giemsa. observation at 10 x ma ... | 2007 | 17374981 |
| larval viability and serological response in horses with long-term trichinella spiralis infection. | the horse is considered an aberrant host for the nematode parasite trichinella spiralis, and many aspects of the biology and epidemiology of trichinella infection in the horse are poorly understood. it has been reported that experimentally-infected horses produce a transient serological response to infection and that muscle larvae are cleared more rapidly than in parasite-adapted hosts such as the pig and humans. however, limited numbers of animals have been studied, and both the longevity of la ... | 2007 | 17386976 |
| trichinella spiralis reinfection: macrophage activity in balb/c mice. | the role of macrophages and their products--nitric oxide (no) and superoxide anion (o(2) (-))--were examined in balb/c mice reinfected with trichinella spiralis. mice were infected twice with 400 t. spiralis larvae; the secondary infection was performed 60 days after the primary one. adult t. spiralis in intestines were detected from 5 to 20 days postprimary infection (dpi), and postreinfection (dpri), but the intensity of worm expulsion was greater and quicker after the reinfection. the highest ... | 2007 | 17393182 |
| concurrent infection with trichinella spiralis and other helminths in pigs. | the aim of this study was to investigate possible influence of different helmintosis in the development of trichinella spiralis in experimental infected pigs. forty-two iberian pigs were allocated to six groups. three groups were single inoculated with ascaris suum, metastrongylus apri or t. spiralis, respectively. two groups were co-infected with t. spiralis and a. suum or t. spiralis and m. apri, respectively, while the last group included uninfected control pigs. clinical signs were only obse ... | 2007 | 17400390 |
| trichinella spiralis-outbreak in the slovak republic. | trichinellosis is a zoonosis caused by nematode worms of the genus trichinella and acquired through the consumption of raw or undercooked meat or meat products that harbour parasite larvae. the present report was designed to evaluate the course and circumstances surrounding the trichinellosis outbreak that occurred due to consumption of pork from a backyard pig in the southwest area of slovakia in 2001. | 2007 | 17401712 |
| viability and infectivity of trichinella spiralis muscle larvae in frozen horse tissue. | many aspects of the biology and epidemiology of trichinella infection in the horse are poorly understood, including survival of trichinella spp in horse muscle. in this study, we have assessed the freeze tolerance of t. spiralis in horse meat stored at 5, -5, and -18 degrees c for 1 day to 24 weeks. results demonstrate a steady reduction in the number of live ml recovered from the cold stored meat samples. on day 1, recovery of live larvae had been reduced by 18.6%, 50.1%, and 37.2%, and by 4 we ... | 2007 | 17418492 |
| epidemiology, histopathology, and muscle distribution of trichinella t9 in feral raccoons (procyon lotor) and wildlife of japan. | the prevalences of trichinella t9 in trapped raccoons (procyon lotor) and several other potential mammalian reservoirs in hokkaido, wakayama, and nagasaki prefectures were investigated. muscle samples were collected from 2003 to 2006 from 1,080 raccoons, 113 raccoon dogs including 2 species (nyctereutes procyonoides albus and n. p. viverrinus), 41 wild boars (sus scrofa leucomystax), 14 martens (martes melampus), 10 badgers (meles meles), 5 siberian weasels (martes sibirica coreana), 7 mink (mus ... | 2007 | 17310398 |
| irritable bowel syndrome in persons who acquired trichinellosis. | the postinfectious irritable bowel syndrome (pi-ibs) frequently follows bacterial infections. since people suffering from pi-ibs and trichinella spiralis-infected mice develop similar findings, this animal model has been successfully used for pi-ibs studies; however, ibs has never been studied in humans after trichinellosis. the aim of this study was to evaluate the ibs symptoms in people who had acquired trichinellosis. | 2007 | 17313500 |
| binding and repression of translation of the cognate mrna by trichinella spiralis thymidylate synthase differ from the corresponding interactions of the human enzyme. | 2007 | 17326266 | |
| carrier-specificity of a phosphorylcholine-binding antibody requires the presence of the constant domains and is not dependent on the unique vh49 glycine or vh30 threonine residues. | bacterially-produced antibody fragments, such as single-chain fv (scfv) which comprises the variable regions of the light (vl) and heavy (vh) chains joined together by a short flexible linker, are useful as diagnostic and therapeutic agents. we previously constructed a scfv fragment from a hybridoma antibody (mab2) but it unexpectedly lacked the unique carrier specificity of the native antibody. thus, it bound indiscriminately to various phosphorylcholine (pc)-associated antigens, whereas the hy ... | 2007 | 17331532 |
| dendritic cell expression of the transcription factor t-bet regulates mast cell progenitor homing to mucosal tissue. | the transcription factor t-bet was identified in cd4(+) t cells, and it controls interferon gamma production and t helper type 1 cell differentiation. t-bet is expressed in certain other leukocytes, and we recently showed (lord, g.m., r.m. rao, h. choe, b.m. sullivan, a.h. lichtman, f.w. luscinskas, and l.h. glimcher. 2005. blood. 106:3432-3439) that it regulates t cell trafficking. we examined whether t-bet influences homing of mast cell progenitors (mcp) to peripheral tissues. surprisingly, we ... | 2007 | 17296784 |
| prolactin-like hormone in the nematode trichinella spiralis larvae. | expression of prolactin (prl) or prolactin-like hormone has been reported in invertebrates. we investigated the larval phase of trichinella spiralis: (a) to express 23 kda prl, (b) to define its localization and (c) to test its possible biological activity. immunostaining in isolated larvae demonstrated positive material to 23 kda prl by all along the stichosome, specifically in the stichocytes. homogenized immunoblot larvae showed a 23 kda protein band. to assess prl release and its biological ... | 2007 | 17300781 |
| cyclic amp signaling contributes to neural plasticity and hyperexcitability in ah sensory neurons following intestinal trichinella spiralis-induced inflammation. | trichinella spiralis infection causes hyperexcitability in enteric after-hyperpolarising (ah) sensory neurons that is mimicked by neural, immune or inflammatory mediators known to stimulate adenylyl cyclase (ac)/cyclic 3',5'-adenosine monophosphate (camp) signaling. the hypothesis was tested that ongoing modulation and sustained amplification in the ac/camp/phosphorylated camp related element binding protrein (pcreb) signaling pathway contributes to hyperexcitability and neuronal plasticity in g ... | 2007 | 17307183 |
| clinical, haematological, biochemical and economic impacts of trichinella spiralis infection in pigs. | the purpose of this work was to assess the clinical, haematological and biochemical responses of pigs experimentally inoculated with trichinella spiralis. groups of three pigs were inoculated per os with 100, 500 and 5000 t. spiralis muscle larvae, two pigs were used as control. clinical evaluation of disease in pigs included daily examination, rectal temperature measurements and cardiac and respiration rates. haematological studies included: hematocrit (%), hemoglobin (g/dl), and white cell, ne ... | 2007 | 17543458 |
| il-10 regulates movement of intestinally derived cd4+ t cells to the liver. | diseases that affect the intestine may have hepatic manifestations, but the mechanisms involved in establishing hepatic disease secondarily remain poorly understood. we previously reported that il-10 knockout (ko) mice developed severe necrotizing hepatitis following oral infection with trichinella spiralis. in this study, we used this model of intestinal inflammation to further examine the role of il-10 in regulating hepatic injury. hepatic damage was induced by migrating newborn larvae. by del ... | 2007 | 17548634 |
| immunization against trichinellosis using microwaved larvae of trichinela spiralis. | the present study evaluated the effect of microwave irradiation on infective larvae of trichinella spiralis (t. spiralis) by the ultrastructure changes of the microwaved larvae (ml) using scanning electron microscope (sem). the ml tested the ability to immunize mice against a challenge infection with t. spiralis. for the optimal dose and the best route of immunization inducing protection against challenge infection, two doses were used; 300 & 600 ml as one or two-dose regimen, each dose was give ... | 2007 | 17580572 |
| [an epidemic of trichinellosis in autumn 2004 in slavonski brod]. | trichinellosis is a worldwide zoonotic disease caused by a nematode trichinella sp. cases of trichinella spiralis infection in slavonski brod were investigated. a total of 64 trichinellosis cases were notified to the institute of public health, brod-posavska county between october and november 2004. there were 64 patients, 32 of them female, age range 3-67, mean 35 years. the mean incubation period was 3 (range 1-5) weeks. the epidemic started in october and terminated 39 days later. the patient ... | 2007 | 17585481 |
| synthesis and antitrichinellosis activity of some bis(benzimidazol-2-yl)amines. | novel bis(benzimidazol-2-yl)amines were synthesized using two methods and studied for antitrichinellosis activity. dft calculations were performed in order to determine the geometry of molecules. all derivatives of 2-aminobenzimidazole exhibited higher activity in vitro against trichinella spiralis larvae in regard to the activity of albendazole, moreover compounds 4f-i manifested antitrichinellosis effect, which surpassed five times the activity of albendazole. the in vivo screening of intestin ... | 2007 | 17600722 |
| human trichinellosis from laos. | 2007 | 17617851 | |
| [effect of different adjuvant formulations on the induced protection of mice immunized with recombinant protein ts87 of trichinella spiralis]. | to study the effect of 6 formulations of adjuvants on immunoprotection in mice induced by the recombinant ts87 protein (rts87) of trichinella spiralis. | 2007 | 17633818 |
| seroprevalence of trichinella spiralis and toxoplasma gondii in pigs from different housing systems in the netherlands. | prevalences of parasitic infections in pigs from different housing systems may vary, due to their contact with the environment, and this might have consequences for food safety. in this study, 40 organic, 9 free-range and 24 intensive farms were selected and a total of 845 serum samples were tested for antibodies specific for toxoplasma and trichinella using elisa assays. the overall seroprevalence of toxoplasma in the total number of 845 serum samples tested is 2.6%, ranging from 0.38% in inten ... | 2007 | 17646053 |
| identification of stage-specifically expressed genes of trichinella spiralis by suppression subtractive hybridization. | summarynewborn larvae (nbl) and adult (ad) stage-specifically expressed genes or members of gene families of trichinella spiralis were identified by suppression subtractive hybridization (ssh). six cdna clones were identified as nbl stage-specific, including 1 member of the t. spiralis gene family encoding glutamic acid-rich proteins, 2 clones encoding novel serine proteases, 2 closely related clones encoding proteins that are members of a deoxyribonuclease ii (dnase ii)-like family and 1 clone ... | 2007 | 17475093 |
| [about two cases of trichinellosis caused by trichinella britovi]. | trichinellosis is a cosmopolitan zoonosis due to a nematode threadworm, trichinella, essentially trichinella spiralis. human cases mostly appeared sporadically, sometimes endemically, related with consumption of larva stinking meat. we report two cases of trichinellosis, including a myocarditis, caused by trichinella britovi after consumption of frozen wild boar meat. | 2007 | 17502306 |
| inhibition of autoimmune type 1 diabetes by gastrointestinal helminth infection. | gastrointestinal nematode infections are prevalent worldwide and are potent inducers of t helper 2 responses with the capacity to modulate the immune response to heterologous antigens. parasitic helminth infection has even been shown to modulate the immune response associated with autoimmune diseases. nonobese diabetic (nod) mice provide a model for studying human autoimmune diabetes; as in humans, the development of diabetes in nod mice has been linked to the loss of self-tolerance to beta cell ... | 2007 | 17043101 |
| cytokine modulation of muscarinic receptors in the murine intestine. | the extent to which gut motility and smooth muscle contractility are altered by intestinal inflammation depends on the nature of the underlying immune activation. the muscarinic receptor on smooth muscle plays a critical role in mediating acetylcholine-driven motor function. we examined the ability of cytokines to influence muscarinic receptor characteristics on intestinal longitudinal muscle and related the findings to studies on carbachol-induced contraction. cells were isolated from longitudi ... | 2007 | 17431212 |
| the role of il-4, il-13 and il-4ralpha in the development of protective and pathological responses to trichinella spiralis. | t helper type 2 (th2) responses have been shown to be important in protective responses to gastrointestinal (gi) helminth infections and in the development of the intestinal pathology accompanying expulsion of the parasite. different inbred mouse strains have been shown to develop different cytokine profiles following infection with gi helminths with increased resistance observed in those strains where th2 cytokines predominate. the aim of this study was to determine the role of il-4, il-13 and ... | 2007 | 17241396 |
| thermally induced and developmentally regulated expression of a small heat shock protein in trichinella spiralis. | a cdna encoding a small heat shock protein of trichinella spiralis, ts-shsp, was cloned and expressed and is herein characterized. this cdna encoded a predicted protein of 165 amino acids, which had a high sequence identity in alpha crystallin domain with various small heat shock proteins of other organisms. a western blot analysis indicated that anti-ts-shsp recombinant antibody recognized the protein of adults and larvae migrating at about 19 kda. an in situ localization study showed the prote ... | 2007 | 17268805 |
| detection of coproantibodies and faecal immune complexes in human trichinellosis. | trichinella spiralis is the nematode causative agent of trichinellosis, an intestinal and tissular parasitosis. even though an early diagnosis during the intestinal phase is essential to limit the infection in humans, to date, there are no available tests to achieve this goal. based on the immune response generated by the host's intestinal mucosa, the aim of this work was to develop elisas to assess the presence of coproantigens (cags), coproantibodies (cabs) and faecal immune complexes in stool ... | 2007 | 17156581 |
| pro-oxidant effects of mebendazole in albino rats experimentally infected with trichinella spiralis. | trichinellosis treated with mebendazole often leads to complications in the course of the disease in humans and animals as a result of intoxication and hyper-sensitization of an organism due to the massive destruction of parasites. this study was conducted to research mebendazole incidence on lipid peroxidation processes (lpp) in rats' blood in trichinella spiralis-infected and parasite-free albino rats. the research was conducted to evaluate erythrocyte superoxide dismutase (sod) activity and m ... | 2007 | 17186274 |
| coordinated control of immunity to muscle stage trichinella spiralis by il-10, regulatory t cells, and tgf-beta. | we previously demonstrated that il-10 is critical in the control of acute inflammation during development of trichinella spiralis in the muscle. in this study, we use gene-targeted knockout mice, adoptive transfer of specific t cell populations, and in vivo ab treatments to determine the mechanisms by which inflammation is controlled and effector t cell responses are moderated during muscle infection. we report that cd4(+)cd25(-) effector t cells, rather than cd4(+)cd25(+) regulatory t cells, su ... | 2007 | 17202367 |
| secretion and processing of a novel multi-domain cystatin-like protein by intracellular stages of trichinella spiralis. | the excretory-secretory (es) proteins of nematode parasites are of major interest as they function at the host-parasite interface and are likely to have roles crucial for successful parasitism. furthermore, the es proteins of intracellular nematodes such as trichinella spiralis may also function to regulate gene expression in the host cell. in a recent proteomic analysis we identified a novel secreted cystatin-like protein from t. spiralis l1 muscle larva. here we show that the protein, mcd-1 (m ... | 2007 | 17081632 |
| protein profile analysis from advanced third-stage larvae (advl3) and adult worms of gnathostoma binucleatum (nematoda: spirurida). | proteins from crude extracts of advanced third-stage larvae and adult gnathostoma binucleatum nematode worms showed protein profiles in sds-page analysis similar to echinococcus granulosus, trichinella spiralis, dipylidium caninum, ancylostoma caninum, ascaris lumbricoides and toxocara canis. the immunoblot analysis of the human serum infected or suspected to be infected with g. binucleatum using the total larvae extract recognized the 40, 60, 80 and 115 kda proteins and using the total adult wo ... | 2007 | 17096141 |
| isolation of a 14 kda antigen from taenia solium cyst fluid by hplc and its evaluation in enzyme linked immunosorbent assay for diagnosis of porcine cysticercosis. | a fraction with a major band of 14kda was obtained from crude cyst fluid of taenia solium cysticerci by 2-step chromatography. a first fraction isolated by gel filtration (sephacryl s-300 high resolution) was purified using an anion exchange column (mono q hr 5/5) on high performance liquid chromatography. evaluation of the analytic sensitivity of this fraction (f3) was carried out in an antibody enzyme linked immunosorbent assay (ab-elisa-f3) using serum samples from pigs experimentally infecte ... | 2007 | 17101161 |
| early pulmonary response in rats infected with trichinella spiralis. | the migratory stage of trichinella spiralis, the newborn larva, travels along the pulmonary microvascular system on its way to the striated muscle cells. in the present study, an important inflammatory reaction was observed on days 5 and 14 post-infection (p.i.) in the lungs of infected rats. this inflammation was characterized by a th2 cell phenotype of hyperplastic bronchus-associated lymphoid tissue and by goblet cell hyperplasia. among the inflammatory cells were eosinophils and mast cells s ... | 2007 | 17040611 |
| trichinella spiralis: strong antibody response to a 49 kda newborn larva antigen in infected rats. | in this work, we analyzed the kinetics of anti-trichinella spiralis newborn larva (nbl) antibodies (ab) and the antigenic recognition pattern of nbl proteins and its dose effects. wistar rats were infected with 0, 700, 2000, 4000 and 8000 muscle larvae (ml) and bled at different time intervals up to day 31 post infection (p.i.). ab production was higher with 2000 ml dose and decreased with 8000, 4000 and 700 ml. abs were not detected until day 10, peaked on day 14 for the 2000 ml dose and on day ... | 2007 | 16989813 |
| comparative analysis of the excretory-secretory proteome of the muscle larva of trichinella pseudospiralis and trichinella spiralis. | the nematodes trichinella spiralis and trichinella pseudospiralis are both intracellular parasites of skeletal muscle cells and induce profound alterations in the host cell resulting in a re-alignment of muscle-specific gene expression. while t. spiralis induces the production of a collagen capsule surrounding the host-parasite complex, t. pseudospiralis exists in a non-encapsulated form and is also characterised by suppression of the host inflammatory response in the muscle. these observed diff ... | 2007 | 17007860 |
| molecular epidemiology of trichinella spp. in three baltic countries: lithuania, latvia, and estonia. | meat of domestic pigs and wild boars has been the significant source of emerged human trichinellosis in lithuania, latvia, and estonia over the past two decades. however, there is very little known on the occurrence of trichinella spp. in main wildlife reservoirs and its transmission in domestic and sylvatic cycles in these countries. the present study demonstrated considerably higher endemicity of trichinella spp. in main sylvatic reservoirs (28.9-42% in foxes (vulpes vulpes) and raccoon dogs ( ... | 2007 | 17013647 |
| evaluation of baculovirus-derived recombinant 53-kda protein of trichinella spiralis for detection of trichinella-specific antibodies in domestic pigs by elisa. | the complete gene encoding the 53-kda protein derived from trichinella spiralis was cloned and expressed using a baculovirus-based system. characterization of a purified fusion protein consisting of the 53-kda protein and the glutathione s-transferase protein showed unspecific reactivity with swine pre-immune serum in both enzyme-linked immunosorbent assay (elisa) and western blot analysis. subsequently, a purified c-terminal 6xhis-tagged 53-kda protein was used in an elisa. the evaluation of th ... | 2007 | 17016726 |
| evaluation of effects of albendazole on the kinetics of cytosolic glutathione transferase in skeletal muscles during experimental trichinellosis in mice. | in this study, the effect of trichinellosis as well as the effect of albendazole treatment on the kinetics of substrate saturation of cytosolic glutathione transferase (gst) in the skeletal muscles from infected mice was examined because the idea of multifunctional mode of action of anthelmintic drugs was considered. our results pointed out to the influence of trichinellosis and albendazole treatment on the quaternary structure of gst in mouse muscles. a double reciprocal plot of gst saturation ... | 2007 | 17024361 |
| swine infection with trichinella spiralis: comparative analysis of the mucosal intestinal and systemic immune responses. | the immune protective response developed by swine against trichinella spiralis is not yet fully understood, particularly at the mucosal level. this study aimed to characterise intestinal immunity to t. spiralis by comparison with the systemic response in specifically pathogen-free pigs. for this purpose, the kinetics of cytokine and antibody production were assessed in the intestinal mucosa and serum of swine infected with t. spiralis for up to 60 days post-infection (dpi). an ex vivo model of j ... | 2007 | 16962244 |
| nematodes as host resistance models for detection of immunotoxicity. | greater susceptibility to infection is a hallmark of compromised immune function in humans and animals, and is often considered the benchmark against which the predictive value of immune function tests are compared. the focus of this paper is resistance to infection with the parasitic nematode trichinella spiralis as a model of host resistance. topics include overviews of parasite biology, host immune responses that limit infection and methods used to evaluate the host response to infection. det ... | 2007 | 16938464 |
| trichinella spiralis: characterization of phage-displayed specific epitopes and their protective immunity in balb/c mice. | trichinellosis is a global zoonosis mainly caused by trichinella spiralis. we have previously reported that a novel ts87 gene from the cdna library of adult t. spiralis was cloned and expressed in a prokaryotic expression system. vaccination with recombinant ts87 protein (rts87) induced a muscle larvae burden reduction in balb/c mice by 29% in response to t. spiralis infection. in the present study, we screened a random phage-displayed peptide library using monoclonal antibody 5a3 which recogniz ... | 2008 | 17707815 |
| intestinal secretory and absorptive function in trichinella spiralis mouse model of postinfective gut dysfunction: role of bile acids. | observations showing that bile acid malabsorption is frequent in irritable bowel syndrome (ibs) suggest that alterations in bile acid-induced secretion and absorption could contribute to ibs-associated diarrhoea. the secretory response to bile acids, fluid transport and bile absorption was examined in intestinal tissues from a trichinella spiralis mouse model of postinfectious gut dysfunction in vitro. changes in the protein expression of apical sodium-dependent bile acid transporter (asbt) were ... | 2008 | 17675326 |
| trichinella spiralis induces de novo expression of group ivc phospholipase a2 in the intestinal epithelium. | phospholipase a2 (pla2) enzymes play a central role in the initiation, propagation and resolution of inflammation. here, we describe de novo expression of group ivc pla2 (pla2g4c) within the intestinal epithelium of trichinella spiralis parasitised mice. this mouse mast cell protease-1 sensitive, calcium-independent pla2 is not detectable in the jejunal epithelium of uninfected mice but becomes highly expressed within the epithelial compartment within days of nematode establishment. we propose t ... | 2008 | 18001740 |
| identification and characterization of a full-length cdna encoding paramyosin of trichinella spiralis. | a full-length cdna encoding trichinella spiralis paramyosin (ts-pmy) was cloned by immunoscreening a cdna library of the adult t. spiralis worm. ts-pmy cdna consists of 2655bp that encode 885 amino acids. the recombinant protein (rts-pmy) was expressed and purified by ni-affinity chromatography. western blot analysis showed that rts-pmy could be recognized by sera from t. spiralis-infected humans, swine, rabbits, and mice. immunolocalization demonstrated that ts-pmy was abundant on the surface o ... | 2008 | 18021743 |
| identification, molecular characterisation and differential expression of caveolin-1 in trichinella spiralis maturing oocytes and embryos. | caveolins are integral membrane proteins implicated in cholesterol homeostasis and transport, endocytosis mechanisms and regulation of signal transduction in differentiated cells. in this work a caveolin-1 gene from the nematode trichinella spiralis (ts-cav-1) was cloned and identified as an adult-specific antigen. for this, a cdna library of t. spiralis 3-day-old adult worms was screened using a stage-specific cdna-labelled probe. one positive clone contained a cdna insert of 1427-bp and a full ... | 2008 | 17803998 |
| chronic urticaria and angioedema with concomitant eosinophilic vasculitis due to trichinella infection. | 2008 | 18176764 | |
| a major trichinellosis outbreak suggesting a high endemicity of trichinella infection in northern laos. | trichinellosis is an important and under-recognized food-borne zoonosis in southeast asia. after 30 years of no reports, a small outbreak was described in central lao pdr (laos) in 2003. here we report a large outbreak of at least 650 estimated patients in udomxay (northern laos) in june 2005. trichinella elisa assays on serum from 133 patients and western blot assays on 16 patients were positive in 67.6% and 81.2%, respectively. no deaths were recorded. consumption of uncooked or fermented pork ... | 2008 | 18187783 |
| immunoparasitological evaluation of trichinella spiralis infection during human pregnancy: a small case series. | the clinical background, vertical transmission of trichinella spiralis and helminthotoxic activity of sera were evaluated in four pregnant women infected at different gestation times. parasitological and immunological parameters were analyzed. patients presented clinical symptoms and a seroconversion pattern typical of trichinella infection, independently of the gestation trimester. all patients, including one patient treated with mebendazole, gave birth to healthy infants at full term. studies ... | 2008 | 18455208 |
| mixed infection, trichinella spiralis and trichinella britovi, in a wild boar hunted in the province of cáceres (spain). | the etiological agents of human trichinellosis are distributed worldwide in domestic and wild animals. in spain, two morphologically indistinguishable trichinella species have been described--trichinella spiralis and trichinella britovi--that are perpetuated in both domestic and sylvatic cycles. the present work reports a double natural infection involving these species in a wild boar killed by hunters in the province of cáceres, spain. after artificial digestion of the boar's muscles, nine larv ... | 2008 | 18466901 |
| the complete mitochondrial genomes for three toxocara species of human and animal health significance. | studying mitochondrial (mt) genomics has important implications for various fundamental areas, including mt biochemistry, physiology and molecular biology. in addition, mt genome sequences have provided useful markers for investigating population genetic structures, systematics and phylogenetics of organisms. toxocara canis, toxocara cati and toxocara malaysiensis cause significant health problems in animals and humans. although they are of importance in human and animal health, no information o ... | 2008 | 18482460 |
| physiological changes in the gastrointestinal tract and host protective immunity: learning from the mouse-trichinella spiralis model. | infection and inflammation in the gastrointestinal (gi) tract induces a number of changes in the gi physiology of the host. experimental infections with parasites represent valuable models to study the structural and physiological changes in the gi tract. this review addresses research on the interface between the immune system and gi physiology, dealing specifically with 2 major components of intestinal physiology, namely mucin production and muscle function in relation to host defence, primari ... | 2008 | 18501042 |
| candidate genes responsible for common and different pathology of infected muscle tissues between trichinella spiralis and t. pseudospiralis infection. | the gene expression profiles were compared between trichinella spiralis- and t. pseudospiralis-infected muscle tissues by means of a cdna microarray. out of 30,000 genes, the expressions of 55 genes were up-regulated in both t. spiralis and t. pseudospiralis infections, 24 genes were down-regulated in both trichinella infections, 30 genes were up-regulated only in t. spiralis infection, 23 genes were down-regulated only in t. spiralis infection, 25 genes were up-regulated only in t. pseudospiral ... | 2008 | 18501667 |
| neuromuscular changes in a rat model of colitis. | intracolonic administration of trichinella spiralis larvae in rats causes colitis with features similar to ulcerative colitis, notably with inflammation predominantly limited to the colonic mucosa. our aim was to characterize the functional and neurochemical changes occurring within the myenteric (mp) and submucosal plexuses (smp) during t. spiralis-induced colitis. infected rats had decreased body weight, altered stool consistency and elevated myeloperoxidase activity, 6 and 14 days post-infect ... | 2008 | 18534920 |
| expressed sequence tags of trichinella spiralis muscle stage larvae. | in order to obtain greater insight into the relevant genomic expression patterns of trichinella spiralis, 992 expressed sequence tags (ests) were collected from a cdna library of t. spiralis muscle stage larvae and assembled into 60 clusters and 385 singletons. of them, 445 (44.7%) ests were annotated to their homologous genes, and small fractions were matched to known genes of nematodes. the annotated ests were classified into 25 eukaryotic orthologous groups (kog). cytochrome c oxidase (34 clo ... | 2008 | 18552539 |
| genome evolution in caenorhabditis. | since the completion of the caenorhabditis elegans genome sequence 10 years ago, efforts of the large community of c. elegans geneticists have resulted in a high-quality annotation of the structures and sequence relatedness of nearly all the protein encoding and rna genes. based on increasingly accurate gene counts in other species, it now appears that c. elegans has more functional genes than most insects and approximately the same number as most mammals. in the last few years, draft genome seq ... | 2008 | 18573804 |
| advances in the sequencing of the genome of the adenophorean nematode trichinella spiralis. | the adenophorean nematodes are evolutionarily distant from other species in the phylum nematoda. interspecific comparisons of predicted proteins have supported such an ancient divergence. accordingly, trichinella spiralis represents a basal nematode representative for genome sequencing focused on gaining a deeper insight into the evolutionary biology of nematodes. in addition, molecular characteristics that are conserved across the phylum could be of great value for control strategies with broad ... | 2008 | 18598573 |
| swine trichinella infection and geographic information system tools. | pastured pigs are vulnerable to trichinella spiralis infection through exposure to wild reservoir hosts. to evaluate the potential impact of the expanding production of pork from pasture-raised pigs, we mapped locations of t. spiralis occurrence and pastured-pig farms in the united states. twenty-eight farms were located within 50 km of previous infection. | 2008 | 18598633 |
| characterization of rat bone marrow dendritic cells initially primed by trichinella spiralis antigens. | pathogen-derived products have the capacity to induce maturation of bone marrow-derived dendritic cells (bmdcs)into populations of effectors cells that polarize th cells toward th1 or th2 phenotype via different mechanisms. since those mechanisms are not entirely clear for helminths, and almost completely unknown for trichinella spiralis(ts), we started an investigation of the effects of ts antigens (four different antigens isolated from all three life-cycle stages of parasite)on maturation of b ... | 2008 | 18627506 |
| nurse cell of trichinella spp. as a model of long-term cell cycle arrest. | nurse cell (nc), formed from skeletal muscle cells upon infection with parasitic nematode trichina, presents a rare system of long-term suspension in the cell cycle. signaling pathways and general biological functions of trichinella spiralis nc, inferred from network analysis of competitive expression microarray data (nc vs. c2c12 myoblasts and myotubes), performed in ingenuity pathways analysis (ipa) software and confirmed by real-time pcr, are presented. assuming 4n dna content in nc nuclei, i ... | 2008 | 18635964 |
| [diagnosis and treatment of trichinellosis]. | lack of specific symptoms and signs makes clinical diagnosis of trichinellosis difficult. epidemiological information is important, such as a history of ingesting raw or undercooked meat. an outbreak can be traced to a group of people dining together. usual manifestations include abdominal pain or diarrhea with general discomfort in the enteric stage, and fever, eyelid or facial edema, muscle pain in acute stage. complications, such as myocarditis, pneumonia, encephalitis, may develop in severe ... | 2008 | 18637587 |
| [minimum dose of trichinella spiralis muscle larvae to infect mice]. | seventy male mice (kunming strain) were randomly divided into 7 groups (10 mice per group), each mouse was orally inoculated with 30, 25, 20, 15, 10, 5 or 3 muscle stage larvae of trichinella spiralis, respectively. all infected mice were sacrificed 6 weeks post-inoculation, number of larvae per gram (lpg) of diaphragm were counted by compression method (trichinelloscopy), the carcass was digested by artificial digestion method and lpg was counted. the larval detection rate by trichinelloscopy a ... | 2008 | 18637592 |
| detection of trichinella spiralis, t. britovi and t. pseudospiralis in muscle tissue with real-time pcr. | infections caused by trichinella species occur throughout the world in many wild and domestic animals resulting in trichinellosis in men. in europe, domestic pigs are predominantly infected by three trichinella species: t. spiralis, t. britovi and t. pseudospiralis. present methods for detection of trichinella spp. (compressorium method, artificial digestion) do not always sufficiently recognize trichinella larvae and these techniques are labor-intensive, time consuming and do not differentiate ... | 2008 | 18639594 |
| towards a risk-based surveillance for trichinella spp. in danish pig production. | increasing demands for cost-effectiveness in surveillance for human health hazards from animal origins can be met by introducing risk-based principles. this e.g. implies targeting subpopulations with higher risk of infection compared to the whole population. furthermore, historical data from surveillance can be collated and used to assess future risk of infection. to demonstrate the effectiveness of combining these two approaches, we used a model called "discounting historical evidence". it depe ... | 2008 | 18649961 |
| molecular evidence for a novel encapsulated genotype of trichinella from patagonia, argentina. | at present, trichinella spiralis is the only species of this genus reported from south america. herein, we detail a molecular analysis of a new encapsulated isolate of muscle larvae of trichinella, found in a mountain lion (puma concolor) coming from the patagonia, argentina. we studied three dna regions previously probed to be useful for the identification of all eleven recognized trichinella genotypes: expansion segment 5 (es5), cytochrome c-oxidase subunit i (coi) and 5s ribosomal dna interge ... | 2008 | 18650017 |
| afferent hypersensitivity in a mouse model of post-inflammatory gut dysfunction: role of altered serotonin metabolism. | visceral hypersensitivity is an important clinical feature associated with irritable bowel syndrome which in some patients has been linked to prior infection. here we employ an animal model in which transient infection leads to persistent gut dysfunction to investigate the role of altered 5-ht metabolism upon afferent mechanosensensitivity in the post-infected gut. jejunal segments isolated from trichinella spiralis-infected mice were used to assess 5-ht metabolism whilst afferent activity in t. ... | 2008 | 18653657 |
| spatial distribution of trichinella britovi, t. pseudospiralis and t. spiralis in red foxes (vulpes vulpes) in hungary. | the red fox (vulpes vulpes) is considered one of the main reservoir of trichinella spp. in europe. as limited information on trichinella infection in wildlife of hungary is available, 2116 red foxes, representing more than 3% of the estimated fox population of the country, were screened to detect trichinella larvae by a digestion method. trichinella larvae from the 35 positive foxes were identified by a multiplex pcr as trichinella britovi (30 isolates, 85.7%), trichinella spiralis (4 isolates, ... | 2008 | 18657367 |
| the effectors responsible for gastrointestinal nematode parasites, trichinella spiralis, expulsion in rats. | helper t (th2) cells type 2 have a central role in host protective responses to gastrointestinal nematode parasite, trichinella spiralis infection, but the actual effector mechanisms involved in parasite expulsion are still uncertain. recent evidences suggest that mast cell recruitment and activation may associate with parasite elimination from host intestines in mice. on the other hand, ige production may induce defensive responses to primary infection with the helminth in rats. the differences ... | 2008 | 18677623 |